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You searched for: Keyword "hemagglutination" Remove constraint Keyword: "hemagglutination"
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1. Application of frozen and stored glutaraldehyde-fixed turkey red blood cells for hemagglutination and hemagglutination inhibition assays for the detection and identification of influenza viruses

Author:
Sadhana S. Kode; Shailesh D. Pawar; Deeksha S. Tare; Jayati Mullick
Source:
Journal of virological methods 2021 v.289 pp. 114046
ISSN:
0166-0934
Subject:
hemagglutination,   etc   ; animal ethics; avian influenza; birds; glutaraldehyde; humans; laboratory diagnosis; public health;     Show all 8 Subjects
Abstract:
... Hemagglutination (HA) and hemagglutination inhibition (HI) assays are conventionally used for the detection and identification of influenza viruses, using red blood cells (RBCs) from mammalian and avian sources. However, there could be limitations for availability of fresh RBCs due to situations such as pandemics, public health emergencies, outbreaks in avian species, lack of animal facilities ...
DOI:
10.1016/j.jviromet.2020.114046

2. Assessment of rat polyclonal antisera’s suitability in hemagglutination inhibition assay for influenza surveillance and antigenic mapping

Author:
D.M. Danilenko; M.Y. Eropkin; V.N. Leonenko; N.I. Konovalova; P.A. Petrova; A.I. Zheltukhina; A.D. Vassilieva
Source:
Journal of virological methods 2021 v.293 pp. 114170
ISSN:
0166-0934
Subject:
hemagglutination,   etc   ; Orthomyxoviridae; ferrets; hemagglutination inhibition test; human influenza; monitoring; rats; statistical analysis;     Show all 8 Subjects
Abstract:
... This paper presents comparative hemagglutination inhibition (HI) assay data obtained using ferret or rat antisera to analyze influenza viruses. The results indicate that rat antisera can be successfully applied both for identification and for antigenic analysis of human influenza A and B viruses. Data gained with rat antisera were comparable to those obtained with ferret antisera. In-depth ...
DOI:
10.1016/j.jviromet.2021.114170

3. Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain

Author:
J.L. Arnal; A. Fernández; A.I. Vela; C. Sanz; J.F. Fernández-Garyzábal; D. Cid
Source:
Veterinary microbiology 2021 v.258 pp. 109121
ISSN:
0378-1135
Subject:
hemagglutination,   etc   ; Mannheimia haemolytica; cattle; goats; microbiology; quantitative polymerase chain reaction; respiratory tract diseases; serotypes; sheep; Spain;     Show all 10 Subjects
Abstract:
... This study compares the utility of a commercially available multiplex q-PCR assay for serotyping A1, A2, and A6 M. haemolytica serotypes with indirect hemagglutination, for determining the relative distribution of M. haemolytica capsular types associated with respiratory disorders in cattle, sheep, and goats. For the 129 isolates analyzed, both q-PCR and IHA assays exhibited nearly complete ...
DOI:
10.1016/j.vetmic.2021.109121

4. Formal estimation of the seropositivity cut‐off of the hemagglutination inhibition assay in field diagnosis of influenza D virus in cattle and estimation of the associated true prevalence in Morocco

Author:
Claude Saegerman; Elias Salem; Hicham Ait Lbacha; Said Alali; Zaid Zouagui; Gilles Meyer; Mariette F. Ducatez
Source:
Transboundary and emerging diseases 2021 v.68 no.3 pp. 1392-1399
ISSN:
1865-1674
Subject:
hemagglutination,   etc   ; RNA viruses; bovine respiratory disease; cattle; diagnostic sensitivity; hemagglutination inhibition test; influenza; mutation rate; seroprevalence; viruses; zoonoses; Morocco;     Show all 12 Subjects
Abstract:
... The influenza D virus (IDV) was discovered less than ten years ago. Increased interest in this virus is due to its nature (RNA virus with high mutation rate), its worldwide circulation in livestock species, its probable role in bovine respiratory disease and its zoonotic potential. Until currently, the establishment of positivity cut‐off of the hemagglutination inhibition (HI) assay ...
DOI:
10.1111/tbed.13805

5. Evidence of influenza infection in dogs and cats in central Chile

Author:
Pedro Jimenez-Bluhm; Alejandra Sepulveda; Cecilia Baumberger; Francisca Di Pillo; Soledad Ruiz; Carla Salazar; Victor Marambio; Fernanda Berrios; Pablo Galdames; Alicia Amaro; David Tapia; Bridgett Sharp; Pamela Freiden; Victoria Meliopoulos; Stacey Schultz-Cherry; Christopher Hamilton-West
Source:
Preventive veterinary medicine 2021 v.191 pp. 105349
ISSN:
0167-5877
Subject:
hemagglutination,   etc   ; Influenza A virus; birds; blood; disease transmission; dogs; hemagglutination inhibition test; horses; influenza; monitoring; pandemic; regression analysis; Asia; Chile; North America;     Show all 15 Subjects
Abstract:
... were collected for influenza A virus detection by RT-qPCR, NP-ELISA, and hemagglutination inhibition assay. Logistic regression models were performed to assess the association between NP-ELISA-positivity and variables including sex and animal origin. The percentage of ELISA-positive samples was 43.5 % (95 % CI: 37.0–50.1) and 23.3 % (95 % CI: 10.6–42.7) for dogs and cats, respectively. No association ...
DOI:
10.1016/j.prevetmed.2021.105349

6. Safety and immunogenicity of high doses of quadrivalent influenza vaccine in children 6 months through <18 years of age: A randomized controlled phase II dose-finding trial

Author:
Lee-Jah Chang; Evan J. Anderson; Robert Jeanfreau; Ying He; Bryony Hicks; Anju Shrestha; Aseem Pandey; Victoria Landolfi; Iris DeBruijn; for the QHD04 Study Group
Source:
Vaccine 2021 v.39 no.11 pp. 1572-1582
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; antibodies; confidence interval; hemagglutinins; immunogenicity; influenza vaccines; Canada;     Show all 7 Subjects
Abstract:
... higher for IIV4-HD than IIV4-SD, although most solicited reactions were grade 1 or 2. Hemagglutination inhibition (HAI) and seroneutralization antibody titers were measured 28–35 days after each dose. Geometric mean HAI titers increased with increasing hemagglutinin dose, especially in children 6 months through < 3 years. For IIV4-HD 60 µg, in participants 6 months through < 18 years of age ...
DOI:
10.1016/j.vaccine.2021.02.014

7. A rapid, point-of-care red blood cell agglutination assay detecting antibodies against SARS-CoV-2

Author:
Robert L. Kruse; Yuting Huang; Heather Smetana; Eric A. Gehrie; Timothy K. Amukele; Aaron A.R. Tobian; Heba H. Mostafa; Zack Z. Wang
Source:
Biochemical and biophysical research communications 2021 v.553 pp. 165-171
ISSN:
0006-291X
Subject:
hemagglutination,   etc   ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibodies; blood serum; epitopes; erythrocytes; hemagglutination tests; hospitals; mammals; morbidity; mortality; patients; point-of-care systems; research;     Show all 15 Subjects
Abstract:
... , were designed to bind red blood cells (RBCs) via a single-chain variable fragment (scFv), thereby displaying the receptor-binding domain (RBD) of SARS-CoV-2 spike protein on the surface of RBCs. Mixing mammalian-derived RBD-2E8 and B6–CH1-RBD with convalescent COVID-19 patient serum and RBCs led to visible hemagglutination, indicating the presence of antibodies against SARS-CoV-2 RBD. B6–CH1-RBD made ...
DOI:
10.1016/j.bbrc.2021.03.016

8. Comparative study on the structures of intra- and extra-cellular polysaccharides from Penicillium oxalicum and their inhibitory effects on galectins

Author:
Siying Zhang; Zhonghui Qiao; Zihan Zhao; Jiao Guo; Kaiwen Lu; Kevin H. Mayo; Yifa Zhou
Source:
International journal of biological macromolecules 2021 v.181 pp. 793-800
ISSN:
0141-8130
Subject:
hemagglutination,   etc   ; Penicillium oxalicum; comparative study; galactomannans; galactose; galectins; methylation; mycelium;     Show all 8 Subjects
Abstract:
... , there have been no reports on the fine structure of EPS. Our results of galectin-mediated hemagglutination demonstrate that IPS exhibits greater inhibitory effects on five galectins compared with EPS. In addition, we find that Galf, a five-membered ring form of galactose, can also inhibit galectins. IPS may provide a new source of galectin inhibitors. These results increase our understanding of structure ...
DOI:
10.1016/j.ijbiomac.2021.04.042

9. Preparation and evaluation of virus-like particle vaccine against H3N8 subtype equine influenza

Author:
Pengju Zhang; Yixue Sun; Chengcheng Tan; Mengmeng Ling; Xintao Li; Weixia Wang; Yanlong Cong
Source:
Microbial pathogenesis 2021 v.157 pp. 104885
ISSN:
0882-4010
Subject:
hemagglutination,   etc   ; Influenza A virus; Western blotting; antiserum; equine influenza; horses; immunization; insects; pathogenesis; virus-like particle vaccines; viruses;     Show all 11 Subjects
Abstract:
... To prevent and control H3N8 subtype equine influenza, we prepared virus-like particles (VLPs) comprising the HA, NA and M1 proteins of H3N8 equine influenza virus (EIV) through the insect cell-baculovirus expression system. The results of Western blot and hemagglutination analyses demonstrated that the constructed VLPs comprising HA, NA and M1 proteins have good hemagglutination activity ...
DOI:
10.1016/j.micpath.2021.104885

10. A Review on the Methods Used for the Detection and Diagnosis of Rabbit Hemorrhagic Disease Virus (RHDV)

Author:
Joana Abrantes; Ana M. Lopes
Source:
Microorganisms 2021 v.9 no.5 pp. -
ISSN:
2076-2607
Subject:
hemagglutination tests,   etc   ; Oryctolagus cuniculus; Rabbit hemorrhagic disease virus; cell culture; histology; rabbits; viruses;     Show all 7 Subjects
Abstract:
... from simpler tests initially used, such as the hemagglutination test, to the more recent and sophisticated high-throughput sequencing, along with an overview of their potential and their limitations. ...
DOI:
10.3390/microorganisms9050972

11. Development of a Luminex microbead-based serotyping assay for Glaesserella parasuis

Author:
Sarah Yee; Joanne Meers; Christopher R. Parke; Tamsin S. Barnes; P.J. Blackall; Conny Turni
Source:
Journal of microbiological methods 2021 v.182 pp. 106159
ISSN:
0167-7012
Subject:
hemagglutination,   etc   ; electrophoresis; gels; hybridization; microbeads; polymerase chain reaction; serotypes; virulence;     Show all 8 Subjects
Abstract:
... Glaesserella parasuis consists of 15 serovars with some of them highly virulent and some of them avirulent. As killed vaccines do not provide crossprotection across serovars, serotyping is of importance. Serotyping, previously done by gel diffusion, is now done by multiplex PCR followed by electrophoresis. Accurately differentiating 15 serovars by electrophoresis is problematic. To overcome this p ...
DOI:
10.1016/j.mimet.2021.106159

12. Quantification of lectins in Synsepalum dulcificum and comparison with reference foods

Author:
Adrián Menéndez-Rey; Raquel González-Martos; Peng Ye; Josefa Quiroz-Troncoso; Nicolás Alegría-Aravena; Marta Sánchez-Díez; Ceferino Maestu-Unturbe; Loan Bensadon-Naeder; Carmen Ramírez-Castillejo
Source:
Food chemistry 2021 v.352 pp. 129341
ISSN:
0308-8146
Subject:
hemagglutination,   etc   ; Synsepalum dulcificum; blood; diet; food chemistry; fruits; humans; lectins; physical activity; pinto beans;     Show all 10 Subjects
Abstract:
... A healthy life means a balance between physical activity and a diet rich in fruits and vegetables, however, some plant-based foods can have certain adverse effects due to the presence of anti-nutritional factors, such as lectins, capable of binding molecules and preventing their normal assimilation. The level of lectins in Synsepalum dulcificum fruit was determined by hemagglutination assays ...
DOI:
10.1016/j.foodchem.2021.129341

13. Efficacy of two vaccines against recent emergent antigenic variants of clade 2.3.2.1a highly pathogenic avian influenza viruses in Bangladesh

Author:
Jung-Hoon Kwon; Miria Ferreira Criado; Lindsay Killmaster; Md Zulfekar Ali; Mohammad Giasuddin; Mohammed A. Samad; Md Rezaul Karim; Eric Brum; Md Zakiul Hasan; Dong-Hun Lee; Erica Spackman; David E. Swayne
Source:
Vaccine 2021 v.39 no.21 pp. 2824-2832
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; amino acids; avian influenza; chickens; crows; epitopes; morbidity; mortality; viruses; Bangladesh;     Show all 10 Subjects
Abstract:
... antigenic sites (A-E) and analyzing hemagglutination inhibition (HI) titers with reference antisera. The RE-6 and rHVT-H5 vaccines both conferred 80–100% clinical protection (i.e. reduced morbidity and mortality) against the three challenge viruses with no significant differences in protection. In addition, both vaccines significantly decreased viral shedding from infected chickens as compared ...
DOI:
10.1016/j.vaccine.2021.04.022

14. Human galectin-16 has a pseudo ligand binding site and plays a role in regulating c-Rel-mediated lymphocyte activity

Author:
Yunlong Si; Yuan Yao; Gabriela Jaramillo Ayala; Xumin Li; Qiuyu Han; Wenlu Zhang; Xuejiao Xu; Guihua Tai; Kevin H. Mayo; Yifa Zhou; Jiyong Su
Source:
Biochimica et biophysica acta 2021 v.1865 no.1 pp. 129755
ISSN:
0304-4165
Subject:
hemagglutination,   etc   ; arginine; asparagine; crystallography; erythrocytes; galectins; gel chromatography; humans; lactose; ligands; prototypes;     Show all 11 Subjects
Abstract:
... The structure of human galectin-16 (Gal-16) has yet to be solved, and its function has remained elusive.X-ray crystallography was used to determine the atomic structures of Gal-16 and two of its mutants. The Gal-16 oligomer state was investigated by gel filtration, its hemagglutination activity was determined along with its ability to bind lactose using ITC. The cellular distribution of EGFP ...
DOI:
10.1016/j.bbagen.2020.129755

15. Effect of Anadenanthera colubrina protease inhibitors as an antiinflamatory mediator

Author:
Gracimério J Guarneire; Oswaldo Cardoso Junior; Nerilson Marques Lima; Emanuel Aguilar Santos; Caio Aguiar Schulze; Wellington Pereira Silva; João Pedro Oliveira Batista; Gabriela de Paula Carli; Sandra Br Castro; Caio Cesar S Alves; Alessandra P Carli
Source:
Natural product research 2021 v.35 no.10 pp. 1690-1695
ISSN:
1478-6427
Subject:
hemagglutination,   etc   ; Anadenanthera colubrina; anti-inflammatory activity; cell viability; cytotoxicity; dexamethasone; enzyme activity; polyacrylamide gel electrophoresis; research; trypsin; zeta potential;     Show all 11 Subjects
Abstract:
... ) and in vivo anti-inflammatory potential, inhibition rate of trypsin and hemagglutination activity from protein extract. The results revealed the presence of bands at 14, 21 and 30 kDa in SDS-PAGE, the RP-HPLC-PDA analysis showed peaks at 12, 13, 28 and 40 minutes and MALDI-TOF/MS showed peaks with 3.4, 4.7, 5.6, 9.4 and 11.2 kDa. The protein extracts presented enzymatic activity inhibition of trypsin (IC ...
DOI:
10.1080/14786419.2019.1624962

16. Cationic polymer-modified Alhagi honey polysaccharide PLGA nanoparticles as an adjuvant to induce strong and long-lasting immune responses

Author:
Adelijiang Wusiman; Wenming Jiang; Lin Yu; Tianyu Zhu; Jin He; Zhenguang Liu; Ruonan Bo; Jiaguo Liu; Deyun Wang
Source:
International journal of biological macromolecules 2021 v.177 pp. 370-382
ISSN:
0141-8130
Subject:
hemagglutination,   etc   ; Alhagi; adjuvants; antigens; bioavailability; cytokines; honey; lymph; lymph nodes; polymers; polysaccharides; spleen; vaccines;     Show all 13 Subjects
Abstract:
... -encapsulated PLGA nanoparticles (AHPP) to build positively charged antigen carriers. Among them, H5N1-loaded PEI-AHPP formulation could induce highest hemagglutination inhibition (HI) titer, IgG-subtype, and cytokines, activated dendritic cells (DCs) in lymph nodes, and CD3e⁺CD4⁺ and CD3e⁺CD8a⁺ T cells in the spleen of immunized mice. PEI-AHPP could stimulate DCs to highly express MHCI and MHCII molecules ...
DOI:
10.1016/j.ijbiomac.2021.02.130

17. Jackdaw nestlings rapidly increase innate immune function during the nestling phase but no evidence for a trade-off with growth

Author:
Christian Aastrup; Arne Hegemann
Source:
Developmental and comparative immunology 2021 v.117 pp. 103967
ISSN:
0145-305X
Subject:
hemagglutination,   etc   ; Corvus monedula; adulthood; adults; body size; body weight; haptoglobins; hemolysis; immune system; innate immunity;     Show all 10 Subjects
Abstract:
... life in fast-growing animals such as free-living passerines. We studied development of innate baseline immune function in nestlings of free-living jackdaws Corvus monedula. We measured four immune parameters (hemolysis, hemagglutination, bacterial-killing capacity, haptoglobin concentration) and structural body size (body mass, wing length, tarsus length) at day 12 and day 29 post-hatching. We found ...
DOI:
10.1016/j.dci.2020.103967

18. Immunoglobulin isotype compositions of ABO specific antibodies are dependent on the individual patient blood group and blood group specificity: Results from a healthy donor cohort

Author:
Sunil Daga; Shimon Hussain; Nithya Krishnan; David Lowe; Manjit Braitch; Prashanth Patel; Andrew Bentall; Simon Ball; Daniel A. Mitchell; Robert Higgins; Ian Skidmore; Daniel Zehnder; David Briggs
Source:
Journal of immunological methods 2021 v.494 pp. 113053
ISSN:
0022-1759
Subject:
hemagglutination,   etc   ; antibodies; antibody detection; blood; blood groups; flow cytometry; isotopes; patients;     Show all 8 Subjects
Abstract:
... Antibodies specific for the blood group ABO system antigens are of clinical significance and immunological interest. Routine clinical methods typically employ direct or indirect haemagglutination methods to measure IgM and IgG, respectively. We have developed a simple, single tube method to quantify IgM, IgG, and IgA specific for A and B antigens in order to improve accuracy and reproducibility, a ...
DOI:
10.1016/j.jim.2021.113053

19. Validity of urine-CCA cassette test and indirect haem-agglutination assay (IHA) in the detection of schistosomiasis-mansoni infection relative to microscopic examination

Author:
Enas A. El Saftawy
Source:
Journal of parasitic diseases 2021 v.45 no.1 pp. 285-292
ISSN:
0971-7196
Subject:
hemagglutination,   etc   ; Schistosoma mansoni; adults; antigens; blood serum; immunoaffinity chromatography; microscopy; parasites; schistosomiasis mansoni; urine;     Show all 10 Subjects
Abstract:
... Several immunodiagnostic assays have been commercially presented over the last years as easy diagnostic methods for schistosomiasis using serum or urine samples. The performance of immunochromatographic test (ICT) and indirect hemagglutination assay (IHA) was validated in the identification of active schistosomiasis infection. Detection of circulating cathodic antigen (CCA) of the parasite ...
DOI:
10.1007/s12639-020-01303-8

20. Active-controlled phase III study of an egg-cultivated quadrivalent inactivated split-virion influenza vaccine (GC3110A) in healthy Korean children aged 6–35 months

Author:
Ui Yoon Choi; Ki Hwan Kim; Kyung Yil Lee; Jong-Hyun Kim; Chun Soo Kim; Byung Wook Eun; Hwang Min Kim; Dong Ho Kim; Song Eun Song; Dae Sun Jo; Jin Lee; Sang Hyuk Ma; Kwang Nam Kim; Jin Han Kang
Source:
Vaccine 2021 v.39 no.15 pp. 2103-2109
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; clinical trials; confidence interval; immunogenicity; influenza; influenza vaccines; safety assessment; seroconversion; Korean Peninsula;     Show all 9 Subjects
Abstract:
... wherein we compared the immunogenicity and safety of GC3110A with those of a licensed control TIV. Immunogenicity was evaluated by measuring hemagglutination inhibition titers. The 200 participants enrolled in Part 2 were randomized in a 4:1 ratio to receive GC3110A or control TIV. The study vaccine group met both primary (i.e., the lower limit of 95% confidence interval [CI] of the seroconversion rate ...
DOI:
10.1016/j.vaccine.2021.03.005