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1. A digital data interpretation method for hemagglutination inhibition assay by using a plate reader

Author:
Zhao, Wei; Baldwin, Elizabeth; Cameron, Randall
Source:
Analytical biochemistry 2019
ISSN:
0003-2697
Subject:
hemagglutination; hemagglutination inhibition test,   etc   ; chickens; digital database; erythrocytes; galectins; inhibitory concentration 50;     Show all 7 Subjects
Abstract:
... Hemagglutination inhibition (HAI) assay is a simple method quantifying relative binding activities of glycan-lectin interactions. Currently, interpretation of HAI data remains a manual task depending on visual observation. In this study we developed a digital data reading method for HAI assay by using the area scanning function of a microplate reader. This was based on galectin-3-induced hemagglut ...
DOI:
10.1016/j.ab.2019.02.016

2. Investigation on side-product formation during the synthesis of a lactoferrin-derived lactam-bridged cyclic peptide

Author:
Scala, Maria Carmina; Spensiero, Antonia; Pepe, Giacomo; Bertamino, Alessia; Carotenuto, Alfonso; Grieco, Paolo; Novellino, Ettore; Gomez-Monterrey, Isabel M.; Campiglia, Pietro; Sala, Marina
Source:
Amino acids 2018 v.50 no.10 pp. 1367-1375
ISSN:
0939-4451
Subject:
hemagglutination,   etc   ; Orthomyxoviridae; aspartic acid; cysteine; lactams; lactoferrin;     Show all 6 Subjects
Abstract:
... Bovine lactoferrin C-lobe is able to prevent both influenza virus hemagglutination and cell infection. In particular, it was demonstrated that the fragment ⁴¹⁸SKHSSLDCVLRP⁴²⁹ is a potent antiviral peptide. Therefore, we tried to increase the stability of this fragment through side-chain lactam cyclization of the peptide, S[KHSSLD]CVLRP (1). However, classic strategy involving solid-supported cycli ...
DOI:
10.1007/s00726-018-2612-9

3. Evaluation of the Ph. Eur. anti-A and anti-B assay on a pipetting robot in combination with a camera guided reading mode

Author:
Hausammann, G.J.; Bellac, C.L.; Sänger, M.; Gilgen, M.
Source:
Biologicals 2017 v.47 pp. 76-80
ISSN:
1045-1056
Subject:
hemagglutination,   etc   ; automation; cameras; immunoglobulins; traceability;     Show all 5 Subjects
Abstract:
... The main objective of this study was the standardization of the direct anti-A, anti-B haemagglutination assay for immunoglobulin products in microtitre plates and gelcards by automation on a liquid handling robot. In addition, the evaluation of the pipetted microtitre plates with a computer-controlled camera was investigated and these results were related to titres from visual live reading.The tit ...
DOI:
10.1016/j.biologicals.2017.03.004

4. Immunogenicity and safety of a quadrivalent inactivated influenza vaccine in children 6–59 months of age: A phase 3, randomized, noninferiority study

Author:
Statler, Victoria A.; Albano, Frank R.; Airey, Jolanta; Sawlwin, Daphne C.; Graves Jones, Alison; Matassa, Vince; Heijnen, Esther; Edelman, Jonathan; Marshall, Gary S.
Source:
Vaccine 2019 v.37 no.2 pp. 343-351
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; children; detergents; fever; geometry; immunogenicity; influenza; influenza vaccines; lipid content; seizures; seroconversion; United States;     Show all 12 Subjects
Abstract:
... In the Southern Hemisphere 2010 influenza season, Seqirus’ split-virion, trivalent inactivated influenza vaccine was associated with increased reports of fevers and febrile reactions in young children. A staged clinical development program of a quadrivalent vaccine (Seqirus IIV4 [S-IIV4]; Afluria® Quadrivalent/Afluria Quad™/Afluria Tetra™), wherein each vaccine strain is split using a higher deter ...
DOI:
10.1016/j.vaccine.2018.07.036

5. Safety and immunogenicity of inactivated monovalent influenza A/H1N1 vaccine candidate manufactured in Vietnam

Author:
Thang, Ho Vinh; Huong, Vu Minh; Victor, John C.; Van, Cao Bao; Nga, Nguyen Tuyet; Be, Le Van; Cuong, Nguyen Phu; Tsvetnitsky, Vadim; Neuzil, Kathleen M.; Power, Maureen; Flores, Jorge
Source:
Vaccine 2018 v.36 no.46 pp. 6918-6925
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; adults; fever; immunogenicity; influenza; influenza vaccines; Vietnam;     Show all 7 Subjects
Abstract:
... We tested a new A/H1N1 inactivated influenza vaccine (IIV) manufactured by Institute of Vaccines and Medical Biologics (IVAC), Vietnam in 48 adults in a Phase 1, double-blinded, randomized, placebo-controlled trial. Two doses of unadjuvanted vaccine or placebo were administered three weeks apart. The vaccine was well tolerated with only transient mild local reactions and low-grade fever in a small ...
DOI:
10.1016/j.vaccine.2018.10.013

6. Actinobacillus pleuropneumoniae serotypes in Hungary

Author:
Sárközi, Rita; Makrai, László; Fodor, László
Source:
Acta veterinaria Hungarica 2018 v.66 no.3 pp. 343-349
ISSN:
1588-2705
Subject:
hemagglutination tests,   etc   ; Actinobacillus pleuropneumoniae; biotypes; herds; lungs; serotypes; swine; Hungary;     Show all 8 Subjects
Abstract:
... A total of 255 Actinobacillus pleuropneumoniae isolates were collected from 634 lung samples representing 70 swine herds in Hungary between January 2012 and June 2016. On the basis of the indirect haemagglutination test 77 independent strains were included in the evaluation after the elimination of duplicate or multiple serotypes from the same herd. In the case of 7 herds strains of two different ...
DOI:
10.1556/004.2018.031

7. Novel hemagglutinin-binding sulfated oligofucosides and their effect on influenza virus infection

Author:
Kosono, Shuhei; Kasai, Akihiro; Komaba, Sumika; Matsubara, Teruhiko; Sato, Toshinori; Takahashi, Daisuke; Toshima, Kazunobu
Source:
Chemical communications 2018 v.54 no.54 pp. 7467-7470
ISSN:
1364-548X
Subject:
hemagglutination,   etc   ; Orthomyxoviridae; chemical reactions; fucoidan; hemagglutinins; ligands; moieties;     Show all 7 Subjects
Abstract:
... Among a series of chemically synthesized fucoidan derivatives (1–9), 5 was found for the first time to bind to influenza virus hemagglutinins (HAs) and inhibit hemagglutination activity. In addition, a designed C₃-symmetric tripodal ligand 10, synthesized with three sulfated oligofucoside moieties of 5, exhibited much greater hemagglutination inhibition activity than 5. A plaque assay using MDCK c ...
DOI:
10.1039/c8cc03865a

8. Development of a Hemagglutination Inhibition Assay for Duck Tembusu Virus

Author:
Wang, Xiaolei; Yang, Zhiyuan; Wang, Xiuqing; Duan, Huijuan; Liu, Lixin; Cheng, Huimin; Yang, Chenghuai; Hou, Lidan; Pan, Jie; Zhao, Jicheng; Liu, Yuehuan; Lin, Jian
Source:
Avian diseases 2019 v.63 no.2 pp. 298-301
ISSN:
0005-2086
Subject:
hemagglutination; hemagglutination inhibition test,   etc   ; Anatid herpesvirus 1; Duck hepatitis A virus; Influenza A virus; Newcastle disease virus; Tembusu virus; antibodies; antigens; blood serum; brain; ducks; egg drop syndrome; mice; neonates; neutralization tests; vaccines; viruses;     Show all 18 Subjects
Abstract:
... The HB strain of duck Tembusu virus (DTMUV) propagated in the brains of newborn mice was used to prepare antigens for use in the hemagglutination inhibition (HI) test. Results showed that such prepared antigens are highly specific to the serum samples derived from DTMUV-infected animals. No spurious hemagglutination reactions against serum samples specific to avian influenza virus H5, H7, H9 subty ...
DOI:
10.1637/11954-082018-Reg.1

9. An indirect comparison meta-analysis of AS03 and MF59 adjuvants in pandemic influenza A(H1N1)pdm09 vaccines

Author:
Hauser, Michael I.; Muscatello, David J.; Soh, Annabel C.Y.; Dwyer, Dominic E.; Turner, Robin M.
Source:
Vaccine 2019 v.37 no.31 pp. 4246-4255
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; adjuvants; adults; antigens; children; databases; hemagglutinins; immunogenicity; influenza; influenza vaccines; meta-analysis; pain; pandemic; randomized clinical trials; risk; seroconversion;     Show all 16 Subjects
Abstract:
... Although oil-in-water adjuvants improve pandemic influenza vaccine efficacy, AS03 versus MF59 adjuvant comparisons in A(H1N1)pdm09 pandemic vaccines are lacking.We conducted an indirect-comparison meta-analysis extracting published data from randomised controlled trials in literature databases (01/01/2009–09/09/2018), evaluating immunogenicity and safety of AS03- or MF59-adjuvanted vaccines. We co ...
DOI:
10.1016/j.vaccine.2019.06.039

10. Seroprevalence and risk factors of Toxoplasma gondii infection in domestic raccoon dogs in four provinces in northern China

Author:
Wang, Xin; Qin, Si-Yuan; Liu, Zheng-Liang; Zhang, Xiao-Tian; Cui, Dong-Yang; Li, Jing-Hao; Liu, Yan; Zhao, Quan; Ni, Hong-Bo
Source:
Microbial pathogenesis 2019 v.128 pp. 136-138
ISSN:
0882-4010
Subject:
hemagglutination tests,   etc   ; Nyctereutes procyonoides; Toxoplasma gondii; animals; blood serum; dog diseases; females; males; risk factors; seroprevalence; statistical analysis; China;     Show all 12 Subjects
Abstract:
... Toxoplasma gondii is an obligate intracellular apicomplexan parasite that can infect almost all homoiothermal animals, including domestic raccoon dogs (Nyctereutes procyonoides). However, related reports on T. gondii infection in domestic raccoon dogs were limited in China. Therefore, a serological investigation was undertaken to investigate the seroprevalence and risk factors for T. gondii infect ...
DOI:
10.1016/j.micpath.2018.12.049

11. Distinct molecular evolution of influenza H3N2 strains in the 2016/17 season and its implications for vaccine effectiveness

Author:
Kim, Jin Il; Park, Sehee; Chu, Hyuk; Lee, Ilseob; Bae, Joon-Yong; Yoo, Kirim; Kim, Juwon; Lee, Joo-Yeon; Lee, Namju; Kim, Miseon; Kim, Jun-Sub; Hong, Kyung Wook; Jun, Kyung Ran; Lee, Jeong Nyeo; Kim, Kisoon; Park, Man-Seong
Source:
Molecular phylogenetics and evolution 2019 v.131 pp. 29-34
ISSN:
1055-7903
Subject:
hemagglutination inhibition test,   etc   ; Orthomyxoviridae; antiserum; disease outbreaks; hemagglutinins; influenza; lysine; mutation; pathogens; patients; phenotype; phylogeny; sialidase; vaccines; viruses; Korean Peninsula;     Show all 16 Subjects
Abstract:
... Influenza virus is a respiratory pathogen that causes seasonal epidemics by resulting in a considerable number of influenza-like illness (ILI) patients. During the 2016/17 season, ILI rates increased unusually earlier and higher than previous seasons in Korea, and most viral isolates were subtyped as H3N2 strains. Notably, the hemagglutinin (HA) of most Korean H3N2 strains retained newly introduce ...
DOI:
10.1016/j.ympev.2018.10.042

12. Production performance, immune response and carcass traits of broiler chickens fed diet incorporated with Kappaphycus alvarezii

Author:
Qadri, S. S. N.; Biswas, A.; Mandal, A. B.; Kumawat, M.; Saxena, R.; Nasir, A. M.
Source:
Journal of applied phycology 2019 v.31 no.1 pp. 753-760
ISSN:
0921-8971
Subject:
hemagglutination,   etc   ; Kappaphycus alvarezii; body weight changes; broiler chickens; carcass characteristics; cell-mediated immunity; chicks; experimental diets; feed conversion; feed intake;     Show all 10 Subjects
Abstract:
... This study evaluates the production performance, immune responses and carcass traits in broiler chickens (0 to 42 days) fed diet with incorporated Kappaphycus alvarezii (AF-KWP). Day-old chicks (280) were randomly distributed into 35 groups with 8 chicks in each group (7 dietary treatments × 5 replicates). Seven experimental diets were formulated by adding 0 (control—T₁), 0.25 AF-KWP (T₂), 0.50 AF ...
DOI:
10.1007/s10811-018-1498-y

13. A comparison of viral microneutralization and haemagglutination inhibition assays as measures of seasonal inactivated influenza vaccine immunogenicity in the first year after reduced intensity conditioning, lymphocyte depleted allogeneic haematopoietic stem cell transplant

Author:
Miller, Paul D.E; de Silva, Thushan I.; Leonard, Hayley; Anthias, Chloe; Hoschler, Katja; Goddard, Kathryn; Peggs, Karl; Madrigal, Alejandro; Snowden, John A.
Source:
Vaccine 2019 v.37 no.3 pp. 452-457
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; at-risk population; geometry; hematopoietic stem cells; immunogenicity; immunosuppression; influenza vaccines; lymphocyte count; patients; seroconversion; vaccination;     Show all 11 Subjects
Abstract:
... Traditionally, immune response to influenza vaccines has been measured using the haemagglutination inhibition (HAI) assay. A broader repertoire of techniques including the sensitive viral microneutralization (VMN) assay is now recommended by the European Medicines Agency (EMA). Comparing HAI and VMN, we determined immune response to a trivalent 2015–2016 seasonal inactivated influenza vaccine (SII ...
DOI:
10.1016/j.vaccine.2018.11.061

14. Modulatory potential on immune system of a new alkaloid isolated from Coccinea cordifolia root

Author:
Deb Roy, Saumendu; Dash, Suvakanta; Dasgupta, Ramesh Kumari; Chakraborty, Jashabir
Source:
International journal of biological macromolecules 2019 v.121 pp. 643-649
ISSN:
0141-8130
Subject:
hemagglutination,   etc   ; Fourier transform infrared spectroscopy; alkaloids; carbon; chromatography; drugs; immune system; levamisole; models; nuclear magnetic resonance spectroscopy; spectral analysis;     Show all 11 Subjects
Abstract:
... In this study, a new alkaloid was isolated from methanolic extract of Coccinea cordifolia root using flash chromatography and was subjected to spectral analysis like 1H NMR, 13C NMR, FTIR analysis and GCMS for confirming the structure of the isolated alkaloid. The isolated alkaloid was also subjected to immunomodulatory assays to verify its role in the claims against the plant. It was found that t ...
DOI:
10.1016/j.ijbiomac.2018.10.104

15. Phytochemical information and pharmacological activities of Okra (Abelmoschus esculentus): A literature‐based review

Author:
Islam, Muhammad Torequl
Source:
Phytotherapy research 2019 v.33 no.1 pp. 72-80
ISSN:
0951-418X
Subject:
hemagglutination,   etc   ; Abelmoschus esculentus; antioxidant activity; bioactive compounds; chemical constituents of plants; databases; fruits; humans; medicinal properties; nutrients; okra; seeds; trypsin;     Show all 13 Subjects
Abstract:
... This review aimed at summarizing phytochemical reports and biological activities of Abelmoschus esculentus L. from the database sources. For this, an up‐to‐date search was made in the PubMed, Science Direct, MedLine, Scopus, and Google Scholar. The findings suggest that A. esculentus contains various nutrients and important phytochemicals. It possesses a number of important biological activities, ...
DOI:
10.1002/ptr.6212

16. Canine trypanosomiasis in an endemic Costa Rican community: Demonstration of the active infection cycle

Author:
Bonilla, Marta C.; Castro-Vásquez, Ruth M.; Herrero-Acosta, Marco Vinicio; Urbina-Villalobos, Andrea; Dolz, Gaby
Source:
Veterinary Parasitology: Regional Studies and Reports 2019 v.17 pp. 100307
ISSN:
2405-9390
Subject:
hemagglutination,   etc   ; Triatominae; cross-sectional studies; dogs; dry season; fluorescent antibody technique; light microscopy; longitudinal studies; polymerase chain reaction; seroconversion; seroprevalence; trypanosomiasis; Costa Rica;     Show all 13 Subjects
Abstract:
... A cross-sectional study was conducted to determine the prevalence of canine trypanosomiasis in an endemic community of Costa Rica. The indirect hemagglutination and indirect immunofluorescence assay yielded positive results in 6.4% (20/314) of canine samples analyzed; polymerase chain reaction (PCR) and light microscopy yielded positive results in one dog. Subsequently, a longitudinal study was ca ...
DOI:
10.1016/j.vprsr.2019.100307

17. A computationally designed H5 antigen shows immunological breadth of coverage and protects against drifting avian strains

Author:
Ross, Ted M.; DiNapoli, Joshua; Giel-Moloney, Maryann; Bloom, Chalise E.; Bertran, Kateri; Balzli, Charles; Strugnell, Tod; Sá e Silva, Mariana; Mebatsion, Teshome; Bublot, Michel; Swayne, David E.; Kleanthous, Harry
Source:
Vaccine 2019 v.37 no.17 pp. 2369-2376
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; Influenza A virus; antibodies; antigens; chickens; ducks; genes; hemagglutinins; humans; influenza vaccines; pandemic; phylogeny; swans; virus-like particle vaccines; viruses;     Show all 15 Subjects
Abstract:
... Since the first identification of the H5N1 Goose/Guangdong lineage in 1996, this highly pathogenic avian influenza virus has spread worldwide, becoming endemic in domestic poultry. Sporadic transmission to humans has raised concerns of a potential pandemic and underscores the need for a broad cross-protective influenza vaccine. Here, we tested our previously described methodology, termed Computati ...
Handle:
10113/6338104
DOI:
10.1016/j.vaccine.2019.03.018

18. Seasonal differences in baseline innate immune function are better explained by environment than annual cycle stage in a year‐round breeding tropical songbird

Author:
Nwaogu, Chima J.; Cresswell, Will; Versteegh, Maaike A.; Tieleman, B. Irene
Source:
The journal of animal ecology 2019 v.88 no.4 pp. 537-553
ISSN:
0021-8790
Subject:
hemagglutination,   etc   ; Pycnonotus; antigens; breeding; dry season; environmental factors; females; haptoglobins; hemolysis; innate immunity; life history; molting; nitric oxide; rain; risk; seasonal variation; songbirds; wet season;     Show all 18 Subjects
Abstract:
... Seasonal variation in innate immunity is often attributed to either temporal environmental variation or to life‐history trade‐offs that arise from specific annual cycle stages but decoupling them is difficult in natural populations. Here, we effectively decouple seasonal environmental variation from annual cycle stage effects by exploiting cross‐seasonal breeding and moult in the tropical Common B ...
DOI:
10.1111/1365-2656.12948

19. Purification and biophysical characterization of a mannose/N-acetyl-d-glucosamine-specific lectin from Machaerium acutifolium and its effect on inhibition of orofacial pain via TRPV1 receptor

Author:
Santos, Ana L.E.; Leite, Gerlânia O.; Carneiro, Romulo F.; Roma, Renato R.; Santos, Valdenice F.; Santos, Maria H.C.; Pereira, Raquel O.; Silva, Rafael C.; Nagano, Celso S.; Sampaio, Alexandre H.; Rocha, Bruno A.M.; Delatorre, Plinio; Campos, Adriana R.; Teixeira, Claudener S.
Source:
Archives of biochemistry and biophysics 2019 v.664 pp. 149-156
ISSN:
0003-9861
Subject:
hemagglutination,   etc   ; Machaerium; ammonium sulfate; analgesic effect; capsaicin; erythrocytes; glycoproteins; ion channels; ion exchange chromatography; lectins; mannose; molecular weight; nociception; pH; pain; proteinases; rabbits; seeds; temperature;     Show all 19 Subjects
Abstract:
... A new mannose/N-acetyl-dglucosamine-specific lectin, named MaL, was purified from seeds of Machaerium acutifolium by precipitation with ammonium sulfate, followed by affinity and ion-exchange chromatography. MaL haemagglutinates either native rabbit erythrocytes or those treated with proteolytic enzymes. MaL is highly stable by the ability to maintain its haemagglutinating activity after exposure ...
DOI:
10.1016/j.abb.2019.02.009

20. Inactivated or damaged? Comparing the effect of inactivation methods on influenza virions to optimize vaccine production

Author:
Herrera-Rodriguez, José; Signorazzi, Aurora; Holtrop, Marijke; de Vries-Idema, Jacqueline; Huckriede, Anke
Source:
Vaccine 2019 v.37 no.12 pp. 1630-1637
ISSN:
0264-410X
Subject:
hemagglutination,   etc   ; Orthomyxoviridae; Toll-like receptor 7; binding capacity; cell lines; formaldehyde; hemagglutinins; inactivated vaccines; influenza; influenza vaccines; pathogenicity; temperature; virion; viruses;     Show all 14 Subjects
Abstract:
... The vast majority of commercially available inactivated influenza vaccines are produced from egg-grown or cell-grown live influenza virus. The first step in the production process is virus inactivation with β-propiolactone (BPL) or formaldehyde (FA). Recommendations for production of inactivated vaccines merely define the maximal concentration for both reagents, leaving the optimization of the pro ...
DOI:
10.1016/j.vaccine.2019.01.086