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immunoassays, etc ; European Union; catalysts; catalytic activity; human health; mobile telephones; mycotoxins; Show all 7 Subjects
Abstract:
... Sensitive, on-site and multiple detection of mycotoxins is a vital early-warning tool to minimize food losses and protect human health and the environment. Although paper-based lateral flow immunoassay (LFIA) has been extensively applied in mycotoxins monitoring, low-cost, portable, ultrasensitive and quantitative detection is still a formidable challenge. Herein, a series of Fe-N-C single-atom na ...
immunoassays, etc ; biotechnology; detection limit; food safety; genetically modified organisms; markets; plasmids; rapid methods; Show all 8 Subjects
Abstract:
... Advanced molecular biotechnology has been developed to provide favorable technical support for genetically modified (GM) crop detection. For sensitive on-site detection, a Cas12a-based immunoassay strip was self-prepared and evaluated in this study. The strip was applied to analyze transgenic screening elements P-CaMV 35S and T-NOS by combination with RPA and Cas12a cleavage reaction. The assay ca ...
immunoassays, etc ; detection limit; fluorescence; food chemistry; furaltadone; immunoaffinity chromatography; metabolites; mica; nanogold; nanoparticles; nitrofurantoin; Show all 11 Subjects
Abstract:
... Nitrofurans such as furaltadone and nitrofurantoin are a type of synthetic broad-spectrum antibiotics. Various fluorescent nanomaterials have been used as labeling materials in immunochromatographic assays (ICAs) for nitrofurans detection. However, previous fluorescent nanomaterials can undergo aggregation-caused quenching, leading to a decrease in the detection sensitivity. In this study, we deve ...
immunoassays, etc ; algorithms; automation; clenbuterol; color; colorimetry; computer vision; food safety; lean meat; lighting; mobile telephones; ractopamine; Show all 12 Subjects
Abstract:
... Ractopamine (RAC) and clenbuterol (CLE) are feed additives with adverse effects of consuming too much to food safety. It is necessary to develop an efficient and accurate colorimetric analysis method for immune-based detection of RAC and CLE. Traditional human-vision-based colorimetric analysis for lateral flow immunoassay (LFIA) is non-quantifiable and low-in-automation, while container-based and ...
immunoassays, etc ; acetochlor; atrazine; catalytic activity; colorimetry; crop production; food chemistry; fruits; high performance liquid chromatography; juices; vegetables; voltammetry; Show all 12 Subjects
Abstract:
... Herbicides atrazine and acetochlor are used in crop production. Because of environmental and health hazards with respective maximum contamination levels of 3 and 20 ng/mL, quantifying these herbicides is important when considering presence in foods and vegetables. We utilized two Pd@Pt nanoparticle-amplified immunoassays, a colorimetric Pd@Pt nanoparticle-linked immunosorbent assay (NLISA) and dif ...
... To determine gentamicin residues in animal tissues, a monoclonal antibody (Mab) was produced and a sensitive indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) was developed. At first, gentamicin was conjugated with bovine serum albumin as immunogens which were used to immunize BALB/c mice. Then, an anti-gentamicin Mab was prepared by hybridoma technology. Finally, a sensitive icCL ...
... Improving detection sensitivity is still a major research emphasis for lateral flow immunoassay (LFIA). Increasing the binding efficiency and stability of the probe is an achievable and effective solution. In this work, we developed a highly sensitive lateral flow immunoassay for clenbuterol detection by using bismuth sulfide nanoparticle (Bi₂S₃) nanoparticles (NPs) as a novel marker. Here, Bi₂S₃ ...
immunoassays, etc ; antibodies; chlorpyrifos; detection limit; dielectric spectroscopy; electrochemistry; food chemistry; food matrix; glyphosate; high fat foods; low fat foods; pesticide residues; portable equipment; Show all 13 Subjects
Abstract:
... On-field detection of pesticide residue in complex-food matrices is a challenge when it comes to analytical detection involving multistep extraction and purification. In this study, we test the feasibility of an electrochemical portable device for detection of spiked pesticides Glyphosate (Glyp) and Chlorpyrifos (Chlp) in low-fat and high-fat food matrix. The immunoassay based two-plex sensing pla ...
... This Special Issue of Foods, Application of Antibody and Immunoassay for Food Safety, contains ten papers that were refereed and selected in accordance with the usual editorial standards of the journal [...] ...
immunoassays, etc ; biomarkers; biosensors; electrochemiluminescence; microgels; microscopy; Show all 6 Subjects
Abstract:
... Bead-based assays are successfully combined with electrochemiluminescence (ECL) technology for detection of a wide range of biomarkers. Herein, we demonstrate a novel approach to enhance the ECL signal by decorating micrometric beads with [Ru(bpy)₃]²⁺-grafted microgels (diameter ∼100 nm). Rapid and stable light emission was spatially resolved at the level of single functionalized beads. An enhance ...
immunoassays, etc ; adulterants; industry; secondary metabolites; toxicity; Show all 5 Subjects
Abstract:
... Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a ...
immunoassays, etc ; dialysis; hypothyroidism; research; thyroxine; veterinary medicine; Show all 6 Subjects
Abstract:
... Measurement of free T4 by analogue immunoassay (fT4a) is popular but its ability to differentiate hypothyroidism from non-thyroidal illness (NTI) is unclear. The aims were to assess fT4a concentrations in dogs with NTI and to explore diagnostic agreement with total T4 and free T4 measured by equilibrium dialysis (fT4d). fT4a was measured in dogs classified with mild, moderate and severe NTI. Total ...
immunoassays, etc ; analytical chemistry; biomarkers; immunohistochemistry; mass spectrometry; Show all 5 Subjects
Abstract:
... Immunoassay is one of the most important clinical techniques for disease/pathological diagnosis. Mass spectrometry (MS) has been a popular and powerful readout technique for immunoassays, generating the mass spectrometric immunoassays (MSIAs) with unbeatable channels for multiplexed detection. The sensitivity of MSIAs has been greatly improved with the development of mass labels from element label ...
immunoassays, etc ; analytical chemistry; biomarkers; ionization; mass spectrometry; Show all 5 Subjects
Abstract:
... The sensitive and accurate determination of macromolecular biomarkers is in high demand for clinical diagnosis. Mass spectrometric immunoassay (MSIA) combines the advantages of immunoassay with mass spectrometry (MS) detection, which offers excellent specificity and sensitivity in bioanalysis and attracts more and more research attentions. Compared with the direct MS analysis, mass tag-based MSIA ...
immunoassays, etc ; bioassays; carbendazim; chemiluminescence; cobalt; detection limit; Show all 6 Subjects
Abstract:
... Single-atom catalysts (SACs) have been applied in various fields as they display extremely high utilization efficiency of catalytic sites. A majority of SACs prepared by high-temperature calcination suffer from poor water dispersion and lose of labelling groups. Herein cobalt SACs (CSACs) were synthesized with a solvothermal method by adopting hybridized MOFs Fe₂O₃/MIL-100(Fe) as the carriers to l ...
immunoassays, etc ; Cryptosporidium parvum; cryptosporidiosis; diarrhea; farms; industrialization; China; Show all 7 Subjects
Abstract:
... Cryptosporidium parvum is a major zoonotic pathogen responsible for outbreaks of severe diarrhoea in humans and calves. Almost all investigations of cryptosporidiosis outbreaks caused by C. parvum have focused on its IIa subtype family in industrialized nations. From December 2018 to April 2019, approximately 200 neonatal calves on a large cattle farm in Hebei Province, China, were diagnosed with ...
immunoassays, etc ; dose response; energy transfer; luminescence; nanoparticles; therapeutics; Show all 6 Subjects
Abstract:
... A homogeneous particle-based immunoassay using upconverting nanoparticles (UCNPs) has been developed for multiplexing potency analysis of two different therapeutic monoclonal antibodies (mAbs) in a fixed-combination formulation.The UCNP, considered as the best donor lumiphore for luminescence resonance energy transfer (LRET), offers long lasting excitation state and increased signal-to-noise (S/N) ...
immunoassays, etc ; adverse effects; biosensors; detection limit; surface area; Show all 5 Subjects
Abstract:
... A lateral flow immunoassay (LFA) technique for sensitive and multiplexed on-site detection of bacteria remains a challenge. Here, we develop a bi-channel surface-enhanced Raman scattering (SERS)-based LFA by using three-dimensional membrane-like SERS tags as nanostickers (named GO@Au/Ag) for direct and ultrasensitive analysis of multiple pathogens in a single test. The grafting of numerous Ag sate ...
immunoassays, etc ; aldehydes; bacterial infections; biomarkers; fluorescence; rapid methods; Show all 6 Subjects
Abstract:
... A fast and highly sensitive amplified luminescent proximity homogeneous assay (AlphaLISA) method was developed for quantitation of plasma heparin-binding protein levels. In this study, a method directly coupling donor and acceptor beads modified with aldehyde groups to anti-HBP antibodies was proposed, which can effectively simplify the steps and shorten the reaction time to achieve faster detecti ...
chemiluminescence immunoassays, etc ; adsorption; biomarkers; drug therapy; inflammation; phycoerythrin; Show all 6 Subjects
Abstract:
... Quantified inflammatory biomarkers are effective clinical strategy for correct and reasonable drug treatment. In the study, a triple lateral flow immunoassay (triple LFIA) had firstly been developed for specific and simultaneous detection of three pivotal inflammatory biomarkers (PCT, CRP and SAA) via biotin-streptavidin-phycoerythrin signal amplification system in one strip. The developed triple ...