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You searched for: Subject "immunoassays" Remove constraint Subject: "immunoassays"
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1. Fe-N-C single-atom nanozyme for ultrasensitive, on-site and multiplex detection of mycotoxins using lateral flow immunoassay

Author:
Xinfa Cai; Fei Ma; Jun Jiang; Xianglong Yang; Zhaowei Zhang; Zelang Jian; Meijuan Liang; Peiwu Li; Li Yu
Source:
Journal of hazardous materials 2023 v.441 pp. 129853
ISSN:
0304-3894
Subject:
immunoassays,   etc   ; European Union; catalysts; catalytic activity; human health; mobile telephones; mycotoxins;     Show all 7 Subjects
Abstract:
... Sensitive, on-site and multiple detection of mycotoxins is a vital early-warning tool to minimize food losses and protect human health and the environment. Although paper-based lateral flow immunoassay (LFIA) has been extensively applied in mycotoxins monitoring, low-cost, portable, ultrasensitive and quantitative detection is still a formidable challenge. Herein, a series of Fe-N-C single-atom na ...
DOI:
10.1016/j.jhazmat.2022.129853

2. An ultra-sensitive test strip combining with RPA and CRISPR/Cas12a system for the rapid detection of GM crops

Author:
Jinbin Wang; Yu Wang; Hua Liu; Xiuwen Hu; Minghao Zhang; Xiaofeng Liu; Hailong Ye; Haijuan Zeng
Source:
Food control 2023 v.144 pp. 109383
ISSN:
0956-7135
Subject:
immunoassays,   etc   ; biotechnology; detection limit; food safety; genetically modified organisms; markets; plasmids; rapid methods;     Show all 8 Subjects
Abstract:
... Advanced molecular biotechnology has been developed to provide favorable technical support for genetically modified (GM) crop detection. For sensitive on-site detection, a Cas12a-based immunoassay strip was self-prepared and evaluated in this study. The strip was applied to analyze transgenic screening elements P-CaMV 35S and T-NOS by combination with RPA and Cas12a cleavage reaction. The assay ca ...
DOI:
10.1016/j.foodcont.2022.109383

3. Dual-color aggregation-induced emission nanoparticles for simultaneous lateral flow immunoassay of nitrofuran metabolites in aquatic products

Author:
Xiuying Liu; Fei Xia; Shaoen Zhang; Yuanyuan Cheng; Ling Fan; Si Kang; Xue Gao; Xiaofei Sun; Jianrong Li; Xuepeng Li; Lijie Zhu
Source:
Food chemistry 2023 v.402 pp. 134235
ISSN:
0308-8146
Subject:
immunoassays,   etc   ; detection limit; fluorescence; food chemistry; furaltadone; immunoaffinity chromatography; metabolites; mica; nanogold; nanoparticles; nitrofurantoin;     Show all 11 Subjects
Abstract:
... Nitrofurans such as furaltadone and nitrofurantoin are a type of synthetic broad-spectrum antibiotics. Various fluorescent nanomaterials have been used as labeling materials in immunochromatographic assays (ICAs) for nitrofurans detection. However, previous fluorescent nanomaterials can undergo aggregation-caused quenching, leading to a decrease in the detection sensitivity. In this study, we deve ...
DOI:
10.1016/j.foodchem.2022.134235

4. A customizable automated container-free multi-strip detection and line recognition system for colorimetric analysis with lateral flow immunoassay for lean meat powder based on machine vision and smartphone

Author:
Guanao Zhao; Sijie Liu; Guo Li; Wentai Fang; Yangjun Liao; Rui Li; Longsheng Fu; Jianlong Wang
Source:
Talanta 2023 v.253 pp. 123925
ISSN:
0039-9140
Subject:
immunoassays,   etc   ; algorithms; automation; clenbuterol; color; colorimetry; computer vision; food safety; lean meat; lighting; mobile telephones; ractopamine;     Show all 12 Subjects
Abstract:
... Ractopamine (RAC) and clenbuterol (CLE) are feed additives with adverse effects of consuming too much to food safety. It is necessary to develop an efficient and accurate colorimetric analysis method for immune-based detection of RAC and CLE. Traditional human-vision-based colorimetric analysis for lateral flow immunoassay (LFIA) is non-quantifiable and low-in-automation, while container-based and ...
DOI:
10.1016/j.talanta.2022.123925

5. Simultaneous detection of two herbicides in fruits and vegetables with nanoparticle-linked immunosorbent and lateral flow immunoassays

Author:
Eunice Y. Kwon; Xiaofan Ruan; Fei Yu; Yuehe Lin; Dan Du; Bernard J. Van Wie
Source:
Food chemistry 2023 v.399 pp. 133955
ISSN:
0308-8146
Subject:
immunoassays,   etc   ; acetochlor; atrazine; catalytic activity; colorimetry; crop production; food chemistry; fruits; high performance liquid chromatography; juices; vegetables; voltammetry;     Show all 12 Subjects
Abstract:
... Herbicides atrazine and acetochlor are used in crop production. Because of environmental and health hazards with respective maximum contamination levels of 3 and 20 ng/mL, quantifying these herbicides is important when considering presence in foods and vegetables. We utilized two Pd@Pt nanoparticle-amplified immunoassays, a colorimetric Pd@Pt nanoparticle-linked immunosorbent assay (NLISA) and dif ...
DOI:
10.1016/j.foodchem.2022.133955

6. Production of high affinity monoclonal antibody and development of indirect competitive chemiluminescence enzyme immunoassay for gentamicin residue in animal tissues

Author:
Peng Dai; Yan Zhang; Yanping Hong; Jianhua Xiong; Huaying Du; Luying Duan; Dan Wang; Yubo Wang; Weijie Deng; Bruce D. Hammock; Wuying Yang
Source:
Food chemistry 2023 v.400 pp. 134067
ISSN:
0308-8146
Subject:
enzyme immunoassays,   etc   ; antigens; bovine serum albumin; chemiluminescence; cross reaction; detection limit; fish; food chemistry; gentamicin; hybridomas; monoclonal antibodies; pork;     Show all 12 Subjects
Abstract:
... To determine gentamicin residues in animal tissues, a monoclonal antibody (Mab) was produced and a sensitive indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) was developed. At first, gentamicin was conjugated with bovine serum albumin as immunogens which were used to immunize BALB/c mice. Then, an anti-gentamicin Mab was prepared by hybridoma technology. Finally, a sensitive icCL ...
DOI:
10.1016/j.foodchem.2022.134067

7. A lateral flow immunoassay based on chemisorbed probes in virtue of hydrogen bond receptors on the Bi2S3 NPs

Author:
Huilan Hu; Yanli Tian; Xuechi Yin; Jing Ren; Lihong Su; Jingke Xu; Conghui Jia; Jianlong Wang; Daohong Zhang
Source:
Food chemistry 2023 v.401 pp. 134133
ISSN:
0308-8146
Subject:
immunoassays,   etc   ; beef; bismuth; clenbuterol; detection limit; food chemistry; food safety; hydrogen; hydrogen bonding; milk; monoclonal antibodies; nanoparticles; pork; sulfides;     Show all 14 Subjects
Abstract:
... Improving detection sensitivity is still a major research emphasis for lateral flow immunoassay (LFIA). Increasing the binding efficiency and stability of the probe is an achievable and effective solution. In this work, we developed a highly sensitive lateral flow immunoassay for clenbuterol detection by using bismuth sulfide nanoparticle (Bi₂S₃) nanoparticles (NPs) as a novel marker. Here, Bi₂S₃ ...
DOI:
10.1016/j.foodchem.2022.134133

8. Pesticide analytical screening system (PASS): A novel electrochemical system for multiplex screening of glyphosate and chlorpyrifos in high-fat and low-fat food matrices

Author:
Durgasha C. Poudyal; Vikram Narayanan Dhamu; Manish Samson; Sriram Muthukumar; Shalini Prasad
Source:
Food chemistry 2023 v.400 pp. 134075
ISSN:
0308-8146
Subject:
immunoassays,   etc   ; antibodies; chlorpyrifos; detection limit; dielectric spectroscopy; electrochemistry; food chemistry; food matrix; glyphosate; high fat foods; low fat foods; pesticide residues; portable equipment;     Show all 13 Subjects
Abstract:
... On-field detection of pesticide residue in complex-food matrices is a challenge when it comes to analytical detection involving multistep extraction and purification. In this study, we test the feasibility of an electrochemical portable device for detection of spiked pesticides Glyphosate (Glyp) and Chlorpyrifos (Chlp) in low-fat and high-fat food matrix. The immunoassay based two-plex sensing pla ...
DOI:
10.1016/j.foodchem.2022.134075

9. Application of Antibody and Immunoassay for Food Safety

Author:
Hongtao Lei; Zhanhui Wang; Sergei A. Eremin; Zhiwei Liu
Source:
Foods 2022 v.11 no.6 pp. -
ISSN:
2304-8158
Subject:
immunoassays,   etc   ; antibodies; food safety;     Show all 3 Subjects
Abstract:
... This Special Issue of Foods, Application of Antibody and Immunoassay for Food Safety, contains ten papers that were refereed and selected in accordance with the usual editorial standards of the journal [...] ...
DOI:
10.3390/foods11060826

10. Enhanced electrochemiluminescence at microgel-functionalized beads

Author:
Dongni Han; Bertrand Goudeau; Véronique Lapeyre; Valérie Ravaine; Dechen Jiang; Danjun Fang; Neso Sojic
Source:
Biosensors & bioelectronics 2022 v.216 pp. 114640
ISSN:
0956-5663
Subject:
immunoassays,   etc   ; biomarkers; biosensors; electrochemiluminescence; microgels; microscopy;     Show all 6 Subjects
Abstract:
... Bead-based assays are successfully combined with electrochemiluminescence (ECL) technology for detection of a wide range of biomarkers. Herein, we demonstrate a novel approach to enhance the ECL signal by decorating micrometric beads with [Ru(bpy)₃]²⁺-grafted microgels (diameter ∼100 nm). Rapid and stable light emission was spatially resolved at the level of single functionalized beads. An enhance ...
DOI:
10.1016/j.bios.2022.114640

11. Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

Author:
Poomraphie Nuntawong; Waraporn Putalun; Hiroyuki Tanaka; Satoshi Morimoto; Seiichi Sakamoto
Source:
Natural medicines 2022 v.76 no.3 pp. 521-545
ISSN:
1340-3443
Subject:
immunoassays,   etc   ; adulterants; industry; secondary metabolites; toxicity;     Show all 5 Subjects
Abstract:
... Phytoproducts are involved in various fields of industry. Small-molecule (Mw < 900 Da) organic compounds can be used to indicate the quality of plant samples in the perspective of efficacy by measuring the necessary secondary metabolites and in the perspective of safety by measuring the adulterant level of toxic compounds. The development of reliable detection methods for these compounds in such a ...
DOI:
10.1007/s11418-022-01605-6

12. Free thyroxine measurement by analogue immunoassay and equilibrium dialysis in dogs with non-thyroidal illness

Author:
Michael Bennaim; Robert E. Shiel; Helen Evans; Carmel T. Mooney
Source:
Research in veterinary science 2022 v.147 pp. 37-43
ISSN:
0034-5288
Subject:
immunoassays,   etc   ; dialysis; hypothyroidism; research; thyroxine; veterinary medicine;     Show all 6 Subjects
Abstract:
... Measurement of free T4 by analogue immunoassay (fT4a) is popular but its ability to differentiate hypothyroidism from non-thyroidal illness (NTI) is unclear. The aims were to assess fT4a concentrations in dogs with NTI and to explore diagnostic agreement with total T4 and free T4 measured by equilibrium dialysis (fT4d). fT4a was measured in dogs classified with mild, moderate and severe NTI. Total ...
DOI:
10.1016/j.rvsc.2022.03.016

13. Highly sensitive and multiplexed mass spectrometric immunoassay techniques and clinical applications

Author:
Shuting Xu; Huwei Liu; Yu Bai
Source:
Analytical and bioanalytical chemistry 2022 v.414 no.18 pp. 5121-5138
ISSN:
1618-2642
Subject:
immunoassays,   etc   ; analytical chemistry; biomarkers; immunohistochemistry; mass spectrometry;     Show all 5 Subjects
Abstract:
... Immunoassay is one of the most important clinical techniques for disease/pathological diagnosis. Mass spectrometry (MS) has been a popular and powerful readout technique for immunoassays, generating the mass spectrometric immunoassays (MSIAs) with unbeatable channels for multiplexed detection. The sensitivity of MSIAs has been greatly improved with the development of mass labels from element label ...
DOI:
10.1007/s00216-022-03945-4

14. Mass tag-based mass spectrometric immunoassay and its bioanalysis applications

Author:
Mingxia Liu; Daiyu Miao; Shaojie Qin; Huwei Liu; Yu Bai
Source:
Trends in analytical chemistry 2022 pp. 116745
ISSN:
0165-9936
Subject:
immunoassays,   etc   ; analytical chemistry; biomarkers; ionization; mass spectrometry;     Show all 5 Subjects
Abstract:
... The sensitive and accurate determination of macromolecular biomarkers is in high demand for clinical diagnosis. Mass spectrometric immunoassay (MSIA) combines the advantages of immunoassay with mass spectrometry (MS) detection, which offers excellent specificity and sensitivity in bioanalysis and attracts more and more research attentions. Compared with the direct MS analysis, mass tag-based MSIA ...
DOI:
10.1016/j.trac.2022.116745

15. Water dispersible cobalt single-atom catalysts with efficient Chemiluminescence enhancement for sensitive bioassay

Author:
Shuai Luo; Jiaqi Gao; Ying Chen; Hui Ouyang; Lin Wang; Zhifeng Fu
Source:
Talanta 2022 v.250 pp. 123732
ISSN:
0039-9140
Subject:
immunoassays,   etc   ; bioassays; carbendazim; chemiluminescence; cobalt; detection limit;     Show all 6 Subjects
Abstract:
... Single-atom catalysts (SACs) have been applied in various fields as they display extremely high utilization efficiency of catalytic sites. A majority of SACs prepared by high-temperature calcination suffer from poor water dispersion and lose of labelling groups. Herein cobalt SACs (CSACs) were synthesized with a solvothermal method by adopting hybridized MOFs Fe₂O₃/MIL-100(Fe) as the carriers to l ...
DOI:
10.1016/j.talanta.2022.123732

16. Cryptosporidiosis outbreak caused by Cryptosporidium parvum subtype IIdA20G1 in neonatal calves

Author:
Zhenjie Zhang; Dongle Su; Xinan Meng; Ruobing Liang; Weijian Wang; Na Li; Yaqiong Guo; Aizhen Guo; Shujing Li; Zengyuan Zhao; Lihua Xiao; Yaoyu Feng
Source:
Transboundary and emerging diseases 2022 v.69 no.2 pp. 278-285
ISSN:
1865-1674
Subject:
immunoassays,   etc   ; Cryptosporidium parvum; cryptosporidiosis; diarrhea; farms; industrialization; China;     Show all 7 Subjects
Abstract:
... Cryptosporidium parvum is a major zoonotic pathogen responsible for outbreaks of severe diarrhoea in humans and calves. Almost all investigations of cryptosporidiosis outbreaks caused by C. parvum have focused on its IIa subtype family in industrialized nations. From December 2018 to April 2019, approximately 200 neonatal calves on a large cattle farm in Hebei Province, China, were diagnosed with ...
DOI:
10.1111/tbed.13976

17. Development of a multiplexing potency assay using upconverting nanoparticles-based luminescence resonance energy transfer

Author:
Jin-Hee Han; Timothy Toner; Rico Gunawan
Source:
Journal of immunological methods 2022 v.510 pp. 113364
ISSN:
0022-1759
Subject:
immunoassays,   etc   ; dose response; energy transfer; luminescence; nanoparticles; therapeutics;     Show all 6 Subjects
Abstract:
... A homogeneous particle-based immunoassay using upconverting nanoparticles (UCNPs) has been developed for multiplexing potency analysis of two different therapeutic monoclonal antibodies (mAbs) in a fixed-combination formulation.The UCNP, considered as the best donor lumiphore for luminescence resonance energy transfer (LRET), offers long lasting excitation state and increased signal-to-noise (S/N) ...
DOI:
10.1016/j.jim.2022.113364

18. Ultrasensitive and multiplex detection of four pathogenic bacteria on a bi-channel lateral flow immunoassay strip with three-dimensional membrane-like SERS nanostickers

Author:
Chongwen Wang; Chaoguang Wang; Jiaxuan Li; Zhijie Tu; Bing Gu; Shengqi Wang
Source:
Biosensors & bioelectronics 2022 v.214 pp. 114525
ISSN:
0956-5663
Subject:
immunoassays,   etc   ; adverse effects; biosensors; detection limit; surface area;     Show all 5 Subjects
Abstract:
... A lateral flow immunoassay (LFA) technique for sensitive and multiplexed on-site detection of bacteria remains a challenge. Here, we develop a bi-channel surface-enhanced Raman scattering (SERS)-based LFA by using three-dimensional membrane-like SERS tags as nanostickers (named GO@Au/Ag) for direct and ultrasensitive analysis of multiple pathogens in a single test. The grafting of numerous Ag sate ...
DOI:
10.1016/j.bios.2022.114525

19. Development and application of amplified luminescent proximity homogeneous assay for quantitation of heparin-binding protein

Author:
Zhongyi Xiang; Zhibin Zhang; Xindong Chen; Lele Zhang; Xiumei Zhou; Yuan Qin; Xueqin Zhao; Yigang Wang; Biao Huang; Huqiang Tang
Source:
Analytical biochemistry 2022 v.657 pp. 114906
ISSN:
0003-2697
Subject:
immunoassays,   etc   ; aldehydes; bacterial infections; biomarkers; fluorescence; rapid methods;     Show all 6 Subjects
Abstract:
... A fast and highly sensitive amplified luminescent proximity homogeneous assay (AlphaLISA) method was developed for quantitation of plasma heparin-binding protein levels. In this study, a method directly coupling donor and acceptor beads modified with aldehyde groups to anti-HBP antibodies was proposed, which can effectively simplify the steps and shorten the reaction time to achieve faster detecti ...
DOI:
10.1016/j.ab.2022.114906

20. Triple quantitative detection of three inflammatory biomarkers with a biotin-streptavidin-phycoerythrin based lateral flow immunoassay

Author:
Xiao-Ming Wang; Shan Li; Lin-Hai Li; Jian-Xun Song; Yan-Hua Lu; Zhi-Wei Zhou; Lei Zhang
Source:
Analytical biochemistry 2022 v.657 pp. 114915
ISSN:
0003-2697
Subject:
chemiluminescence immunoassays,   etc   ; adsorption; biomarkers; drug therapy; inflammation; phycoerythrin;     Show all 6 Subjects
Abstract:
... Quantified inflammatory biomarkers are effective clinical strategy for correct and reasonable drug treatment. In the study, a triple lateral flow immunoassay (triple LFIA) had firstly been developed for specific and simultaneous detection of three pivotal inflammatory biomarkers (PCT, CRP and SAA) via biotin-streptavidin-phycoerythrin signal amplification system in one strip. The developed triple ...
DOI:
10.1016/j.ab.2022.114915