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1. Paper-based point-of-care immunoassays: Recent advances and emerging trends

Author:
Li, Fei; You, Minli; Li, Shaoxiong; Hu, Jie; Liu, Chang; Gong, Yan; Yang, Huayuan; Xu, Feng
Source:
Biotechnology advances 2020 v.39 pp. 107442
ISSN:
0734-9750
Subject:
immunoassays,   etc   ; commercialization; hospitals; point-of-care systems;     Show all 4 Subjects
Abstract:
... Immunoassay has been routinely used in hospitals and central labs. Nowadays, to further meet the requirement of widespread applications of immunoassays, it is urgently needed to produce a simplified, rapid and low-cost immunoassay to perform tests on site. To this end, paper-based point-of-care (POC) immunoassays have attracted intensive interests in recent years. In this paper, we present a compr ...
DOI:
10.1016/j.biotechadv.2019.107442

2. Validation of the ADVIA Centaur® XP system for the determination of insulin and its application

Author:
Xu, Yu-Ying; Xu, Su-Mei; Li, Xiao-Min; Li, Dai; Yan, Juan; Xu, Ping-Sheng
Source:
Analytical biochemistry 2020 v.591 pp. 113567
ISSN:
0003-2697
Subject:
immunoassays,   etc   ; blood serum; chemiluminescence; humans; insulin; pharmacokinetics;     Show all 6 Subjects
Abstract:
... In this study, a direct chemiluminescent immunoassay for the determination of human serum insulin levels using the ADVIA Centaur® XP system was validated. Dilution recovery, linearity, precision, sensitivity, between analyzer variation, reference interval and stability were analyzed. The linear range of the insulin assay was from 0.64 to 277.27 mU/L. Intra- and inter-assay coefficients of variatio ...
DOI:
10.1016/j.ab.2019.113567

3. Fabrication of chemiluminescence resonance energy transfer platform based on nanomaterial and its application in optical sensing, biological imaging and photodynamic therapy

Author:
Chen, Jia; Qiu, Hongdeng; Zhao, Shulin
Source:
Trends in analytical chemistry 2020 v.122 pp. 115747
ISSN:
0165-9936
Subject:
immunoassays,   etc   ; bioimaging; chemiluminescence; energy transfer; nanomaterials; photochemotherapy;     Show all 6 Subjects
Abstract:
... Chemiluminescence resonance energy transfer (CRET), which involves nonradiative energy transfer from a chemiluminescent donor to a suitable acceptor molecule without the use of an external light source, has drawn considerable attention in diverse fields. This review thoroughly summarizes the latest progress (from 2014 to 2019) of CRET based on nanomaterial in many aspects including small molecule ...
DOI:
10.1016/j.trac.2019.115747

4. Measurement of cholecystokinin in plasma with reference to nutrition related obesity studies

Author:
Rehfeld, Jens F.
Source:
Nutrition research 2020 v.76 pp. 1-8
ISSN:
0271-5317
Subject:
immunoassays,   etc   ; blood proteins; cholecystokinin; digestive system; gastrins; nutrition; obesity; satiety;     Show all 8 Subjects
Abstract:
... This review describes the premises for accurate measurement of the gut hormone and satiety factor cholecystokinin (CCK) in circulation. Such a description is useful for nutrition and obesity research in which CCK in its satiety role has evoked considerable interest during the last decades. The background for the review is two sorts of considerations or concerns. First, CCK is a complex peptide sys ...
DOI:
10.1016/j.nutres.2020.01.003

5. UV-Laser Interference Lithography for Local Functionalization of Plasmonic Nanostructures with Responsive Hydrogel

Author:
Quilis, Nestor Gisbert; Hageneder, Simone; Fossati, Stefan; Auer, Simone K.; Venugopalan, Priyamvada; Bozdogan, Anil; Petri, Christian; Moreno-Cencerrado, Alberto; Toca-Herrera, Jose Luis; Jonas, Ulrich; Dostalek, Jakub
Source:
Journal of physical chemistry 2020 v.124 no.5 pp. 3297-3305
ISSN:
1932-7455
Subject:
immunoassays,   etc   ; benzophenones; biochemical compounds; biosensors; fluorescence; hydrogels; ligands; moieties; nanogold;     Show all 9 Subjects
Abstract:
... A novel approach to local functionalization of plasmonic hotspots at gold nanoparticles with biofunctional moieties is reported. It relies on photocrosslinking and attachment of a responsive hydrogel binding matrix by the use of a UV interference field. A thermoresponsive poly(N-isopropylacrylamide)-based (pNIPAAm) hydrogel with photocrosslinkable benzophenone groups and carboxylic groups for its ...
DOI:
10.1021/acs.jpcc.9b11059
PubMed:
32089762
PubMed Central:
PMC7032879

6. High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) Immunosensor for detection of sulfathiazole in honey

Author:
Cervera-Chiner, Lourdes; Jiménez, Yolanda; Montoya, Ángel; Juan-Borrás, Marisol; Pascual, Nuria; Arnau, Antonio; Escriche, Isabel
Source:
Food control 2020 v.115 pp. 107296
ISSN:
0956-7135
Subject:
immunoassays,   etc   ; antibiotics; detection limit; guidelines; honey; immunosensors; monoclonal antibodies; quartz crystal microbalance; sulfathiazole;     Show all 9 Subjects
Abstract:
... In this study, a piezoelectric immunosensor based on High Fundamental Frequency Quartz Crystal Microbalance (HFF-QCMD) technology was developed for detection of sulfathiazole in honey. The biorecognition was based on a competitive immunoassay in the conjugate-coated format, using monoclonal antibodies as specific immunoreagents. The quantification of sulfathiazole was performed by building the cor ...
DOI:
10.1016/j.foodcont.2020.107296

7. Polydopamine nanospheres as high-affinity signal tag towards lateral flow immunoassay for sensitive furazolidone detection

Author:
Liu, Sijie; Dou, Leina; Yao, Xiaolin; Zhang, Wentao; Zhao, Bingxin; Wang, Zonghan; Ji, Yanwei; Sun, Jing; Xu, Baocheng; Zhang, Daohong; Wang, Jianlong
Source:
Food chemistry 2020 v.315 pp. 126310
ISSN:
0308-8146
Subject:
immunoassays,   etc   ; adsorption; biosensors; carcinogenicity; chemical species; detection limit; furazolidone; humans; metabolites; monitoring; mutagens; nanospheres;     Show all 12 Subjects
Abstract:
... Currently, the low sensitivity and poor binding stability of detection probe prepared via electrostatic adsorption have become the dilemmas of colloidal gold-based lateral flow immunoassays (Au-LFIAs). In this connection, polydopamine nanospheres (PDA NPs) with an eminent covalent connectivity property were introduced as a promising substitute to improve the stability of probe and sensitivity of L ...
DOI:
10.1016/j.foodchem.2020.126310

8. Comparison of a novel chemiluminescence immunoassay with the passive agglutination method for the diagnosis of Mycoplasma pneumoniae infection in children

Author:
He, Shiyi; Yang, Maochun; Wu, Xiaoning; Cai, Guoping; Jiang, Kongmei; Xie, Li
Source:
Journal of microbiological methods 2020 v.173 pp. 105921
ISSN:
0167-7012
Subject:
chemiluminescence immunoassays,   etc   ; Mycoplasma pneumoniae; agglutination; antibodies; blood serum; children; pneumonia; rapid methods; regression analysis; risk factors;     Show all 10 Subjects
Abstract:
... The purpose of this study was to evaluate a newly developed chemiluminescence immunoassay (CLIA) and compare it to passive agglutination (PA) for the diagnosis of pneumonia caused by Mycoplasma pneumoniae in children.A total of 291 children suspected of M. pneumoniae infections were enrolled. Serum samples were obtained from routine diagnostic requests, and specific antibodies were simultaneously ...
DOI:
10.1016/j.mimet.2020.105921

9. The reference method influence on the sensitivity of the Clostridium difficile enzyme immunoassays: A meta analysis

Author:
Martins, João Paulo; Felgueiras, Miguel; Santos, Rui
Source:
Journal of microbiological methods 2020 v.173 pp. 105912
ISSN:
0167-7012
Subject:
enzyme immunoassays,   etc   ; Clostridium difficile; algorithms; diarrhea; gene amplification; meta-analysis; screening; toxins;     Show all 8 Subjects
Abstract:
... The use of enzyme immunoassays to screen for toxins A and B produced by Clostridium difficile is a common procedure in algorithms designed for its detection. Moreover, the absence of a unique test capable of providing reliable results at low cost motivates a great discussion about which algorithm is the best. Thus, several studies have evaluated the performance of these enzyme immunoassays. Howeve ...
DOI:
10.1016/j.mimet.2020.105912

10. Psychosomatic conditions in university students related to melatonin biological rhythm and cortisol morning levels

Author:
Nagane, Mitsuo; Oyama, Yoshinori; Suge, Rie; Watanabe, Shu-Ichi
Source:
Biological rhythm research 2020 v.51 no.4 pp. 543-551
ISSN:
1744-4179
Subject:
enzyme immunoassays,   etc   ; anxiety; biological rhythms; college students; cortisol; fluorescence; lifestyle; melatonin; youth;     Show all 9 Subjects
Abstract:
... The purpose of this pilot study was to explore the lifestyle of today’s youth by identifying characteristics of melatonin and cortisol that affect biological rhythms in university students. We investigated the relationships between morning cortisol levels and psychosomatic conditions to examine the effects of biological rhythm on psychosomatic conditions and daytime sleepiness. To analyse biologic ...
DOI:
10.1080/09291016.2018.1543636

11. Small size nanoparticles—Co3O4 based lateral flow immunoassay biosensor for highly sensitive and rapid detection of furazolidone

Author:
Su, Lihong; Wang, Lulu; Yao, Xiaolin; Yin, Xuechi; Zhang, Han; Zhao, Man; Liu, Sijie; Wang, Zonghan; Wang, Jianlong; Zhang, Daohong
Source:
Talanta 2020 v.211 pp. 120729
ISSN:
0039-9140
Subject:
immunoassays,   etc   ; biosensors; cobalt oxide; color; detection limit; furazolidone; metabolites; nanogold; nanoparticles; probability; rapid methods;     Show all 11 Subjects
Abstract:
... Lateral flow immunoassay (LFIA) biosensor is a paper-based tool and widely utilized in various fields. Here, we developed a novel LFIA biosensor by introducing Co₃O₄ nanoparticles (NPs) as signal labels for highly sensitive detection of 3-amino-2-oxazolidinone (AOZ), a metabolite of furazolidone. The characteristic brown color of Co₃O₄ NPs enabled AOZ to be visually detected by the LFIA. Significa ...
DOI:
10.1016/j.talanta.2020.120729

12. High-throughput chemiluminescence immunoassay based on Co2+/hemin synergistic catalysis for sensitive detection tetrabromobisphenol A bis(2-hydroxyethyl) ether in the environments

Author:
Gu, Lantian; Zou, Yanmin; Li, Yanshen; Zeng, Kun; Zhu, Nuanfei; Zhu, Fang; Gyimah, Eric; Yakubu, Salome; Meng, Hui; Zhang, Zhen
Source:
The Science of the total environment 2020 v.714 pp. 136880
ISSN:
0048-9697
Subject:
chemiluminescence immunoassays,   etc   ; antibodies; catalytic activity; chemiluminescence; cobalt; detection limit; enzyme-linked immunosorbent assay; soil sampling; China;     Show all 9 Subjects
Abstract:
... Here, a novel chemiluminescence (CL) immunoassay was fabricated for sensitive determination of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE), one of typical tetrabromobisphenol A derivatives. At the indirectly competitive method, the synthesized PS@hemin@Co²⁺ was labelled by secondary antibody (Ab₂) instead of common natural enzymes, which showed excellent catalysis towards the deco ...
DOI:
10.1016/j.scitotenv.2020.136880

13. Quantum dots for Förster Resonance Energy Transfer (FRET)

Author:
Cardoso Dos Santos, Marcelina; Algar, W. Russ; Medintz, Igor L.; Hildebrandt, Niko
Source:
Trends in analytical chemistry 2020 v.125 pp. 115819
ISSN:
0165-9936
Subject:
immunoassays,   etc   ; energy transfer; fluorescence; image analysis; nucleic acids; photostability; quantum dots; spectroscopy;     Show all 8 Subjects
Abstract:
... The analysis of biomolecular interactions using quantum dots (QDs) as both FRET donors and acceptors has become an established technique in the life sciences. This development has been driven by the unique properties of QDs, which include large surfaces for the attachment of biomolecules, high brightness and photostability, strong and spectrally broad absorption, and color-tunability via QD size, ...
DOI:
10.1016/j.trac.2020.115819

14. Fast immunoassay for microfluidic western blotting by direct deposition of reagents onto capture membrane

Author:
Arvin, Natalie E.; Dawod, Mohamed; Lamb, Don T.; Anderson, Jon P.; Furtaw, Michael D.; Kennedy, Robert T.
Source:
Analytical methods 2020 v.12 no.12 pp. 1606-1616
ISSN:
1759-9679
Subject:
immunoassays,   etc   ; Western blotting; antibodies; automation; detection limit; electrophoresis; microchip technology; tubulin;     Show all 8 Subjects
Abstract:
... Western blotting is a widely used protein assay platform, but the technique requires long analysis times and multiple manual steps. Microfluidic systems are currently being explored for increased automation and reduction of analysis times, sample volumes, and reagent consumption for western blots. Previous work has demonstrated that proteins separated by microchip electrophoresis can be captured o ...
DOI:
10.1039/d0ay00207k

15. Functional nanomaterials based immunological detection of aflatoxin B₁: a review

Author:
Zha, Y.-H.; Zhou, Y.
Source:
World mycotoxin journal 2020 v.13 no.2 pp. 151-162
ISSN:
1875-0796
Subject:
immunoassays,   etc   ; aflatoxin B1; carcinogenicity; cost effectiveness; food safety; mutagens; nanomaterials; teratogenicity;     Show all 8 Subjects
Abstract:
... Aflatoxin B₁ (AFB₁) is highly carcinogenic, mutagenic and teratogenic. Accordingly, sensitive, rapid and cost-effective techniques for detection of AFB₁ is in urgent demand for food safety and the health of consumers. In this review, we report the current state of immunoassay formats and development, mainly based on nanomaterials for determination of AFB₁. Following an introduction of the field, t ...
DOI:
10.3920/WMJ2019.2504

16. Research progress on chemiluminescence immunoassay combined with novel technologies

Author:
Xiao, Qin; Xu, Chunxia
Source:
Trends in analytical chemistry 2020 v.124 pp. 115780
ISSN:
0165-9936
Subject:
chemiluminescence immunoassays,   etc   ; antibodies; chemiluminescence; diagnostic techniques; drug evaluation; environmental monitoring; nanomaterials; oligonucleotides;     Show all 8 Subjects
Abstract:
... Chemiluminescence immunoassay (CLIA) has been greatly developed in the past several decades due to its good sensitivity and specificity. Nowadays, CLIA has been highly improved with novel nanomaterials and newly-developed technologies. The advancement of CLIA combined with relevant technologies are reviewed in the paper, including enhanced chemiluminescent, antibody preparation, aptamer selection, ...
DOI:
10.1016/j.trac.2019.115780

17. Ultrasensitive Visualization of Virus via Explosive Catalysis of an Enzyme Muster Triggering Gold Nano-aggregate Disassembly

Author:
Xiong, Ling-Hong; Huang, Suibin; Huang, Yalan; Yin, Feng; Yang, Fan; Zhang, Qian; Cheng, Jinquan; Zhang, Renli; He, Xuewen
Source:
ACS applied materials & interfaces 2020 v.12 no.11 pp. 12525-12532
ISSN:
1944-8252
Subject:
immunoassays,   etc   ; Enterovirus A; catalytic activity; crosslinking; detection limit; human health; nanogold; peptides; viruses;     Show all 9 Subjects
Abstract:
... Sensitive and accurate diagnosis of viral infection is important for human health and social safety. Herein, by means of explosive catalysis from an enzyme muster, a powerful naked-eye readout platform has been successfully constructed for ultrasensitive immunoassay of viral entities. Liposomes were used to encapsulate multiple enzymes into an active unit. In addition, its triggered rupture could ...
DOI:
10.1021/acsami.9b23247

18. Developments in mycotoxin analysis: an update for 2018-19

Author:
Tittlemier, S.A.; Cramer, B.; Dall’Asta, C.; Iha, M.H.; Lattanzio, V.M.T.; Maragos, C.; Solfrizzo, M.; Stranska, M.; Stroka, J.; Sumarah, M.
Source:
World mycotoxin journal 2020 v.13 no.1 pp. 3-24
ISSN:
1875-0796
Subject:
immunoassays,   etc   ; Alternaria; aflatoxins; analytical methods; ergot alkaloids; fumonisins; ochratoxins; patulin; trichothecenes; zearalenone;     Show all 10 Subjects
Abstract:
... This review summarises developments on the analysis of various matrices for mycotoxins that have been published in the period from mid-2018 to mid-2019. Analytical methods to determine aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxins, patulin, trichothecenes, and zearalenone are covered in individual sections. Advances in sampling strategies are also discussed in a dedicated ...
DOI:
10.3920/WMJ2019.2535

19. Quantum dot nanoconjugates for immuno-detection of circulating cell-free miRNAs

Author:
Shandilya, Ruchita; Sobolev, Aleksander M.; Bunkar, Neha; Bhargava, Arpit; Goryacheva, Irina Yu; Mishra, Pradyumna Kumar
Source:
Talanta 2020 v.208 pp. 120486
ISSN:
0039-9140
Subject:
immunoassays,   etc   ; antibodies; electrophoresis; flow cytometry; fluorescence; fluorometry; gene expression regulation; microRNA; proteins; quantum dots;     Show all 10 Subjects
Abstract:
... Argonaute protein (AGO2) bound circulating cell-free miRNAs (ccf-miRs), in the recent years, has attracted great attention due to their differential abundance in biological fluids. In the present work, a selective and technically uncomplicated quantum dot (QD) nanoconjugate has been fabricated combining the specific affinity of the antibody and fluorescent property of QDs for the precise immuno-de ...
DOI:
10.1016/j.talanta.2019.120486

20. Intrinsic “Turn-On” Aptasensor Detection of Ochratoxin A Using Energy-Transfer Fluorescence

Author:
Armstrong-Price, Delaney E.; Deore, Prashant S.; Manderville, Richard A.
Source:
Journal of agricultural and food chemistry 2020 v.68 no.7 pp. 2249-2255
ISSN:
1520-5118
Subject:
immunoassays,   etc   ; aptasensors; detection limit; energy transfer; fluorescence; humans; instrumentation; ochratoxin A; oligonucleotides; red wines;     Show all 10 Subjects
Abstract:
... Ochratoxin A (OTA) is an intrinsically fluorescent phenolic mycotoxin that contaminates a wide range of food products and is a serious health threat to animals and humans. An OTA binding aptamer (OTABA) that folds into an antiparallel G-quadruplex (GQ) in the absence and presence of target OTA has been incorporated into a vast variety of aptasensor platforms for OTA detection. The development of a ...
DOI:
10.1021/acs.jafc.9b07391