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1. The advanced paraffin-section preparation technique based on multiple cumulus-oocyte complexes rather than ovaries in ovine

Author:
Namei, Erge; Sun, Wei; Pan, Deng; Zhao, Yufen; Yang, Bingxue; Weng, Yu; Du, Chenguang; Li, Haijun; Yu, Boyang; Subudeng, Gerile
Source:
Reproductive Biology 2021 v.21 no.1 pp. 100473
ISSN:
1642-431X
Subject:
immunohistochemistry,   etc   ; oocytes; protein synthesis; sheep;     Show all 4 Subjects
Abstract:
... Immunohistochemical staining is the important method for the identification of protein expression in mammal ovaries, in particular in the follicles with the potential to develop into cumulus-oocyte complexes (COCs), which are able to support oocyte maturation regardless of in vivo or in vitro. Here, we reported an advanced immunohistochemical method based on an artificial structure gathering multi ...
DOI:
10.1016/j.repbio.2020.100473

2. Antlerogenic stem cells extract accelerate chronic wound healing: a preliminary study

Author:
Kmiecik, Janusz; Kulus, Michał Jerzy; Popiel, Jarosław; Cekiera, Agnieszka; Cegielski, Marek
Source:
BMC complementary medicine and therapies 2021 v.21 no.1 pp. 158
ISSN:
2662-7671
Subject:
immunohistochemistry,   etc   ; alternative medicine; phototherapy; statistics; wound treatment;     Show all 5 Subjects
Abstract:
... BACKGROUND: Chronic wounds constitute a significant medical and social problem. Chronic wound treatment may be supported by various techniques, such as negative pressure therapy, phototherapy or stem cells therapy, yet most of those supporting therapies need more evidence to be used for standard wound care. Current study covers the use of sonicated Antlerogenic Stem Cells (ASC) extract on chronic ...
DOI:
10.1186/s12906-021-03336-9

3. Immunohistochemical Screening of HER2 in Canine Carcinomas: A Preliminary Study

Author:
Brunetti, Barbara; Bacci, Barbara; Sarli, Giuseppe; Pancioni, Elisa; Muscatello, Luisa Vera
Source:
Animals 2021 v.11 no.4
ISSN:
2076-2615
Subject:
immunohistochemistry,   etc   ; carcinogenesis; dogs; humans; intestines; therapeutics;     Show all 6 Subjects
Abstract:
... HER2 overexpression has been reported in various human and canine tumours. The aim of this study is to investigate the expression of HER2 protein in different histotypes of canine carcinomas in order to identify potential tumours that could benefit from the HER2-targeted therapy. Eighty-two (82) canine carcinomas (squamous cell, gastro-intestinal, rectal, pulmonary, prostatic, urothelial, and ovar ...
DOI:
10.3390/ani11041006

4. Histological and Immunohistochemical Evaluation of Phosphorylated Mechanistic Target of Rapamycin in Canine Skin Tumours

Author:
Mathew, Raimon; Sajitha, Indira S.; Balakrishnan-Nair, Dhanush K.; Nair, Sudheesh S.; Kariyil, Bibu J.; Abraham, Mammen J.
Source:
Journal of comparative pathology 2021 v.184 pp. 60-64
ISSN:
0021-9975
Subject:
immunohistochemistry,   etc   ; dogs; epithelium; humans; metabolism; rapamycin; therapeutics;     Show all 7 Subjects
Abstract:
... The mechanistic target of rapamycin (mTOR) plays an important role in cellular functions, including growth and metabolism. Recently, mTOR and the activated phosphorylated form of mTOR (p-mTOR) have been reported as potential prognostic markers in many human tumours. However, there are few studies on its activation in canine tumours. We investigated the expression of p-mTOR in 17 canine skin tumour ...
DOI:
10.1016/j.jcpa.2021.02.001

5. Immunohistochemical study of dissociation and association of adherens junctions in splenic sinus endothelial cells

Author:
Uehara, Kiyoko; Uehara, Akira
Source:
Cell and tissue research 2021 v.384 no.1 pp. 25-33
ISSN:
0302-766X
Subject:
immunohistochemistry,   etc   ; dissociation; electron microscopy; research; sphingosine;     Show all 5 Subjects
Abstract:
... It is not yet clear whether cellular junctions between splenic sinus endothelial cells are open or closed. In order to clarify this, immunolocalization of thrombomodulin (TM), endothelial protein C receptor (EPCR), protease-activated receptor 1 (PAR1), sphingosine 1-phosphate receptor 1 (S1P₁), β-catenin phosphorylated at Try142 (β-catenin Y142) and β-catenin phosphorylated at Try654 (β-catenin Y6 ...
DOI:
10.1007/s00441-021-03426-7

6. Pathological and Immunohistochemical Characterization of Thyroid Neoplasms in Cats

Author:
Argenta, Fernando F.; de Mello, Lauren S.; Slaviero, Mônica; Cony, Fernanda G.; Bandinelli, Marcele B.; Pavarini, Saulo P.; Driemeier, David; Sonne, Luciana
Source:
Journal of comparative pathology 2021 v.184 pp. 44-55
ISSN:
0021-9975
Subject:
immunohistochemistry,   etc   ; carcinoma; genes; thyroglobulin; transcription factors; Brazil;     Show all 6 Subjects
Abstract:
... There are few pathological and immunohistochemical descriptions of thyroid neoplasms in cats. We investigated neoplasms of the thyroid in 31 cats (average age 15.6 years) in Southern Brazil. Most (96.8%) of these cases were classified as follicular adenomas and were predominantly unilateral and multinodular. Histologically, macrofollicular adenomas were the most common type observed. Carcinomas re ...
DOI:
10.1016/j.jcpa.2021.01.013

7. p53, ER, and Ki67 Expression in Canine Mammary Carcinomas and Correlation With Pathological Variables and Prognosis

Author:
Brunetti, Barbara; Bacci, Barbara; Angeli, Cristian; Benazzi, Cinzia; Muscatello, Luisa Vera
Source:
Veterinary pathology 2021 v.58 no.2 pp. 325-331
ISSN:
1544-2217
Subject:
immunohistochemistry,   etc   ; animal pathology; dogs; estrogen receptors; prognosis;     Show all 5 Subjects
Abstract:
... Using immunohistochemistry, 170 canine mammary carcinomas were evaluated for p53, ER (estrogen receptor), and Ki67. Of the 170 tumors, 89 were grade I (52.3%), 36 were grade II (21.2%), and 45 were grade III (26.4%). Eight cases (0.5%) were positive for p53 and 69/170 cases (40.5%) were positive for ER. Ki67 values were 24 ± 18% (mean ± SD). Using a cutoff value of 33.3% Ki67-positive neoplastic n ...
DOI:
10.1177/0300985820973462

8. Expression of Semaphorin 3A in Malignant and Normal Bladder Tissue: Immunohistochemistry Staining and Morphometric Evaluation

Author:
Bejar, Ilan; Rubinstein, Jacob; Bejar, Jacob; Sabo, Edmond; Sheffer, Hilla K; Bahouth, Zaher; Halachmi, Sarel; Vadasz, Zahava
Source:
Biology 2021 v.10 no.2
ISSN:
2079-7737
Subject:
immunohistochemistry,   etc   ; bladder; carcinoma; morphometry; mucosa; muscles; urine;     Show all 7 Subjects
Abstract:
... Introduction: Our previous studies showed elevated levels of Semaphorin3a (Sema3A) in the urine of patients with urothelial cancer compared to healthy patients. The aim of this study was to analyze the extent of Sema3A expression in normal and malignant urothelial tissue using immune-staining microscopic and morphometric analysis. Materials and Methods: Fifty-seven paraffin-embedded bladder sample ...
DOI:
10.3390/biology10020109

9. An Immunohistochemical Study of the PTEN/AKT Pathway Involvement in Canine and Feline Mammary Tumors

Author:
Asproni, Pietro; Millanta, Francesca; Ressel, Lorenzo; Podestà, Fabio; Parisi, Francesca; Vannozzi, Iacopo; Poli, Alessandro
Source:
Animals 2021 v.11 no.2
ISSN:
2076-2615
Subject:
immunohistochemistry,   etc   ; cats; dogs; mice; oncogenes; thymoma;     Show all 6 Subjects
Abstract:
... Phosphatase and tensin homolog deleted on chromosome10 (PTEN), phospho-v-Akt murine thymoma viral oncogene homolog (AKT), and the Rapamycin-Insensitive Companion of mTOR (Rictor) expression was investigated by immunohistochemistry in 10 canine mammary adenomas (CMAs), 40 canine mammary carcinomas (CMCs), and 30 feline mammary carcinomas (FMCs). All the CMAs, 25 of 40 CMCs (63%) and 7 of 30 FMCs (2 ...
DOI:
10.3390/ani11020365

10. Olfactory subsystems associated with the necklace glomeruli in rodents

Author:
Zimmerman, Arthur D.; Munger, Steven D.
Source:
Cell and tissue research 2021 v.383 no.1 pp. 549-557
ISSN:
0302-766X
Subject:
immunohistochemistry,   etc   ; epithelium; ganglia; olfactory bulb; research; rodents;     Show all 6 Subjects
Abstract:
... The necklace glomeruli are a loosely defined group of glomeruli encircling the caudal main olfactory bulb in rodents. Initially defined by the expression of various immunohistochemical markers, they are now better understood in the context of the specialized chemosensory neurons of the main olfactory epithelium and Grueneberg ganglion that innervate them. It has become clear that the necklace regi ...
DOI:
10.1007/s00441-020-03388-2

11. Urinary vanillylmandelic acid:creatinine ratio in dogs with pheochromocytoma

Author:
Soler Arias, E.A.; Trigo, R.H.; Miceli, D.D.; Vidal, P.N.; Hernandez Blanco, M.F.; Castillo, V.A.
Source:
Domestic animal endocrinology 2021 v.74 pp. 106559
ISSN:
0739-7240
Subject:
immunohistochemistry,   etc   ; Cushing syndrome; creatinine; domestic animals; endocrinology;     Show all 5 Subjects
Abstract:
... Pheochromocytoma diagnosis in dogs is challenging because biochemical tests are not always available. In humans, urinary vanillylmandelic acid (VMA) is part of a pheochromocytoma biochemical diagnostic profile, whereas its diagnostic accuracy is currently unknown in dogs with pheochromocytoma. Prospectively, VMA was determined by HPLC and expressed as the ratio with respect to urinary creatinine ( ...
DOI:
10.1016/j.domaniend.2020.106559

12. Resveratrol-mediated neurorestoration after cerebral ischemic injury - Sonic Hedgehog signaling pathway

Author:
Yu, Pingping; Wang, Li; Tang, Fanren; Guo, Shuang; Liao, Hongyan; Fan, Cengceng; Yang, Qin
Source:
Life sciences 2021 v.280 pp. 119715
ISSN:
0024-3205
Subject:
immunohistochemistry,   etc   ; apoptosis; astrocytes; cyclopamine; neurites; oligodendroglia; resveratrol; synaptogenesis;     Show all 8 Subjects
Abstract:
... Resveratrol pretreatment can decrease ischemic cerebral injury and enhance proliferation of neural stem cells via mediation of Sonic Hedgehog signaling. However, it is relatively little known about whether neurorestorative effects of resveratrol are mediated by Shh signaling in ischemic cerebral injury. The present study tests whether the Shh signaling pathway mediates resveratrol to promote neuro ...
DOI:
10.1016/j.lfs.2021.119715

13. TRAF2 gene silencing induces proliferation and represses apoptosis of nucleus pulposus cells in rats with intervertebral disc degeneration

Author:
Gu, Mingyong; Zhou, Weijie; Chen, Jianxin; Zhao, Yihui; Xie, Chen; Zhou, Zhenyu
Source:
Life sciences 2021 v.279 pp. 119670
ISSN:
0024-3205
Subject:
immunohistochemistry,   etc   ; Western blotting; animal models; apoptosis; genes; intervertebral disks; phenotype;     Show all 7 Subjects
Abstract:
... We aim to research the role of TRAF2 silencing in regulating proliferation and apoptosis of nucleus pulposus cells (NIPCs) in rats with intervertebral disc degeneration (IDD) through mediating NF-κB signaling pathway.Degenerative disc nucleus pulposus tissues and normal nucleus pulposus tissues were collected to compare the positive expression of TRAF2 protein by immunohistochemistry, and to compa ...
DOI:
10.1016/j.lfs.2021.119670

14. G protein gamma 7 suppresses progression of lung adenocarcinoma by inhibiting E2F transcription factor 1

Author:
Zheng, Hongyu; Tian, Hui; Yu, Xuejuan; Ren, Peng; Yang, Qiuan
Source:
International journal of biological macromolecules 2021 v.182 pp. 858-865
ISSN:
0141-8130
Subject:
immunohistochemistry,   etc   ; Western blotting; adenocarcinoma; carcinogenesis; cell proliferation; lungs; transcription factors;     Show all 7 Subjects
Abstract:
... G protein gamma 7 (GNG7) has been found to be aberrantly expressed in some kinds of malignant tumors. In this study, we mainly discuss the antitumor role of it in lung adenocarcinoma (LUAD) cells. Protein levels of GNG7 in LUAD tissues were measured by western blot and immunohistochemical analysis. Cell proliferation, invasion and migration were detected by CCK-8 assay, 5-ethynyl-2′-deoxyuridine ( ...
DOI:
10.1016/j.ijbiomac.2021.04.082

15. Immunohistochemical expression and prognostic significance of MAGE-A in canine oral malignant melanoma

Author:
Guillén, Alexandra; Stiborova, Katerina; Ressel, Lorenzo; Blackwood, Laura; Finotello, Riccardo; Amores-Fuster, Isabel; Jama, Nimo; Killick, David
Source:
Research in veterinary science 2021 v.137 pp. 226-234
ISSN:
0034-5288
Subject:
immunohistochemistry,   etc   ; antigens; dogs; melanoma; prognosis; research; somatic cells; veterinary medicine;     Show all 8 Subjects
Abstract:
... Canine oral malignant melanoma (COMM) is considered a chemo-resistant cancer with a poor long-term prognosis. The melanoma-associated antigen A (MAGE-A) genes, which belong to the cancer-testis antigen family, are expressed in several different canine cancers but not in normal somatic tissue. This study evaluates the expression of MAGE-A proteins and their prognostic role in COMM.The study was con ...
DOI:
10.1016/j.rvsc.2021.05.009

16. Targeted RNA sequencing of adrenal zones using immunohistochemistry-guided capture of formalin-fixed paraffin-embedded tissue

Author:
Baker, Jessica E.; Plaska, Samuel W.; Qin, Zhaoping; Liu, Chia-Jen; Rege, Juilee; Rainey, William E.; Udager, Aaron M.
Source:
Molecular and cellular endocrinology 2021 v.530 pp. 111296
ISSN:
0303-7207
Subject:
immunohistochemistry,   etc   ; RNA; adrenal glands; endocrinology; gene expression regulation; transcriptomics;     Show all 6 Subjects
Abstract:
... Adequate access to fresh or frozen normal adrenal tissue has been a primary limitation to the enhanced characterization of the adrenal zones via RNA sequencing (RNAseq). Herein, we describe the application of targeted RNAseq to formalin-fixed paraffin-embedded (FFPE) normal adrenal gland specimens. Immunohistochemistry (IHC) was used to visualize and guide the capture of the adrenocortical zones a ...
DOI:
10.1016/j.mce.2021.111296

17. Tocotrienols Ameliorate Neurodegeneration and Motor Deficits in the 6-OHDA-Induced Rat Model of Parkinsonism: Behavioural and Immunohistochemistry Analysis

Author:
Kumari, Mangala; Ramdas, Premdass; Radhakrishnan, Ammu Kutty; Kutty, Methil Kannan; Haleagrahara, Nagaraja
Source:
Nutrients 2021 v.13 no.5
ISSN:
2072-6643
Subject:
immunohistochemistry,   etc   ; Parkinson disease; animal models; brain; inflammation; males; neurons; tocotrienols;     Show all 8 Subjects
Abstract:
... Parkinson’s disease (PD) is a debilitating neurodegenerative disease, which progresses over time, causing pathological depigmentation of the substantia nigra (SN) in the midbrain due to loss of dopaminergic neurons. Emerging studies revealed the promising effects of some nutrient compounds in reducing the risk of PD. One such nutrient compound that possess neuroprotective effects and prevents neur ...
DOI:
10.3390/nu13051583

18. Identification of MCM family as potential therapeutic and prognostic targets for hepatocellular carcinoma based on bioinformatics and experiments

Author:
Lei, Yu; Wang, Shuhui; Liu, Jingmei; Yan, Wei; Han, Ping; Tian, Dean
Source:
Life sciences 2021 v.272 pp. 119227
ISSN:
0024-3205
Subject:
immunohistochemistry,   etc   ; DNA replication; bioinformatics; drugs; hepatoma; prognosis; protein synthesis; therapeutics;     Show all 8 Subjects
Abstract:
... The minichromosome maintenance (MCM) complex is highly conserved, which has drawn increasing attention on physiology and pathology process. However, the role of MCM in hepatocellular carcinoma (HCC) remains largely unclear. We aimed to conduct systematic analysis of expression patterns, prognostic values and potential functions of nine MCM genes in HCC, thus identifying their role in HCC.In our st ...
DOI:
10.1016/j.lfs.2021.119227

19. Native α-Synuclein, 3-Nitrotyrosine Proteins, and Patterns of Nitro-α-Synuclein-Immunoreactive Inclusions in Saliva and Submandibulary Gland in Parkinson’s Disease

Author:
Fernández-Espejo, Emilio; Rodríguez de Fonseca, Fernando; Suárez, Juan; Tolosa, Eduardo; Vilas, Dolores; Aldecoa, Iban; Berenguer, Joan; Damas-Hermoso, Fátima
Source:
Antioxidants 2021 v.10 no.5
ISSN:
2076-3921
Subject:
immunohistochemistry,   etc   ; biomarkers; biopsy; blood serum; cytoplasm; nervous system; saliva;     Show all 7 Subjects
Abstract:
... Background. Salivary α-synuclein (aSyn) and its nitrated form, or 3-nitrotyrosine-α-synuclein (3-NT-αSyn), hold promise as biomarkers for idiopathic Parkinson’s disease (IPD). Nitrative stress that is characterized by an excess of 3-nitrotyrosine proteins (3-NT-proteins) has been proposed as a pathogenic mechanism in IPD. The objective is to study the pathological role of native αSyn, 3-NT-αSyn, a ...
DOI:
10.3390/antiox10050715

20. Phosphorylated Tau protein in the myenteric plexus of the ileum and colon of normothermic rats and during synthetic torpor

Author:
Chiocchetti, R; Hitrec, T; Giancola, F; Sadeghinezhad, J; Squarcio, F; Galiazzo, G; Piscitiello, E; De Silva, M; Cerri, M; Amici, R; Luppi, M
Source:
Cell and tissue research 2021 v.384 no.2 pp. 287-299
ISSN:
0302-766X
Subject:
immunohistochemistry,   etc   ; brain; colon; hibernation; homeostasis; ileum; neurons; phenotype; rats; research;     Show all 10 Subjects
Abstract:
... Tau protein is of primary importance for neuronal homeostasis and when hyperphosphorylated (PP-Tau), it tends to aggregate in neurofibrillary tangles, as is the case with tauopathies, a class of neurodegenerative disorders. Reversible PP-Tau accumulation occurs in the brain of hibernating rodents and it was recently observed in rats (a non-hibernator) during synthetic torpor (ST), a pharmacologica ...
DOI:
10.1007/s00441-020-03328-0