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1. Viroplasms: Assembly and Functions of Rotavirus Replication Factories

Author:
Guido Papa; Alexander Borodavka; Ulrich Desselberger
Source:
Viruses 2021 v.13 no.7 pp. -
ISSN:
1999-4915
Subject:
viral inclusion bodies,   etc   ; Rotavirus; droplets; genome; reverse genetics; separation; virion; virus replication;     Show all 8 Subjects
Abstract:
... Viroplasms are cytoplasmic, membraneless structures assembled in rotavirus (RV)-infected cells, which are intricately involved in viral replication. Two virus-encoded, non-structural proteins, NSP2 and NSP5, are the main drivers of viroplasm formation. The structures (as far as is known) and functions of these proteins are described. Recent studies using plasmid-only-based reverse genetics have si ...
DOI:
10.3390/v13071349

2. Lipid metabolism is involved in the association of rotavirus viroplasms with endoplasmic reticulum membranes

Author:
José L. Martínez; Catherine Eichwald; Elisabeth M. Schraner; Susana López; Carlos F. Arias
Source:
Virology 2022 v.569 pp. 29-36
ISSN:
0042-6822
Subject:
viral inclusion bodies,   etc   ; Rotavirus; biogenesis; endoplasmic reticulum; lipid metabolism; lipids; virology; viruses;     Show all 8 Subjects
Abstract:
... Rotavirus (RV) replication occurs in cytoplasmic membrane-less, electron-dense inclusions termed viroplasms, composed of viral and cellular elements. These inclusions have been shown to colocalize with components of the lipid droplets (LDs), unique organelles that play an essential role in lipid metabolism. Given the robust LDs-viroplasm association, LDs have been proposed to serve as a scaffold f ...
DOI:
10.1016/j.virol.2022.02.005

3. Plasmid-based reverse genetics for probing phosphorylation-dependent viroplasm formation in rotaviruses

Author:
Jeanette M. Criglar; Sue E. Crawford; Mary K. Estes
Source:
Virus research 2021 v.291 pp. 198193
ISSN:
0168-1702
Subject:
viral inclusion bodies,   etc   ; Rotavirus; lipids; mixed infection; mutants; mutation; phosphorylation; research; reverse genetics; serine; virus replication; viruses;     Show all 12 Subjects
Abstract:
... Rotavirus (RV) replication occurs in cytoplasmic compartments, known as viroplasms, that are composed of viral and cellular proteins. Viroplasm formation requires RV nonstructural proteins NSP2 and NSP5 and cellular lipid droplets (LDs); however, the mechanisms required for viroplasm assembly remain largely unknown. We previously identified two conformationally-distinct forms of NSP2 (dNSP2, vNSP2 ...
DOI:
10.1016/j.virusres.2020.198193

4. Silencing suppressors of rice black‐streaked dwarf virus and rice stripe virus hijack the 26S proteasome of Laodelphax striatellus to facilitate virus accumulation and transmission

Author:
Ying Li; Lifei Zhu; Jiaqi Gao; Haoran Ma; Changyuan Li; Yunzhi Song; Xiaoping Zhu; Changxiang Zhu
Source:
Pest management science 2022 v.78 no.7 pp. 2940-2951
ISSN:
1526-498X
Subject:
viral inclusion bodies,   etc   ; Laodelphax striatellus; Rice black streaked dwarf virus; Rice stripe tenuivirus; pest management; proteasome endopeptidase complex; rice; ubiquitination; virus transmission; viruses;     Show all 10 Subjects
Abstract:
... BACKGROUND: Rice black‐streaked dwarf virus (RBSDV) is transmitted by small brown planthopper (Laodelphax striatellus [L. striatellus]) and causes devastating disease in rice. P9‐1 has silencing suppression activity and is the key protein for viroplasm formation in RBSDV‐infected plants and insects; however, its exact function is poorly understood. RESULTS: In this study, the P9‐1 of RBSDV interac ...
DOI:
10.1002/ps.6918

5. IGF1 inclusion bodies: A QbD based process approach for efficient USP as well as early DSP unit operations

Author:
Karl F.J. Metzger; Wolfgang Padutsch; Alexander Pekarsky; Julian Kopp; Alexei M. Voloshin; Harald Kühnel; Michael Maurer
Source:
Journal of biotechnology 2020 v.312 pp. 23-34
ISSN:
0168-1656
Subject:
inclusion bodies,   etc   ; Escherichia coli; anion exchange; chromatography; filtration; pH; protein synthesis; recombinant proteins;     Show all 8 Subjects
Abstract:
... E. coli is an attractive host organism for strong recombinant protein expression. It expresses products either as soluble protein or as inclusion bodies (IB). IBs are insoluble, mostly inactive aggregates. However, recent progress enabled the efficient refolding back into their bioactive structure. Targeted IB production processes have been designed based on their characteristic features such as h ...
DOI:
10.1016/j.jbiotec.2020.02.014

6. Prokaryotic expression of a hub protein of white spot syndrome virus with vaccine potential

Author:
Yongzhong Lu; Linyue Cheng; Fang Wang
Source:
Aquaculture research 2020 v.51 no.9 pp. 3533-3539
ISSN:
1355-557X
Subject:
inclusion bodies,   etc   ; Escherichia coli; White spot syndrome virus; proteins; thioredoxins; vaccines; Arctic region;     Show all 7 Subjects
Abstract:
... Envelope proteins of white spot syndrome virus (WSSV) play an important role in viral entry as well as in triggering host defences. To date, some main envelope proteins such as VP28, VP24 and VP19 have been expressed heterologously and proved effective in WSSV prevention. However, VP62, an envelope protein with hub function as well as better antigenicity, has not been focused on. In an attempt to ...
DOI:
10.1111/are.14690

7. Physico-Chemical Characterization of Permutated Variants of Chaperone GroEL Apical Domain

Author:
M. S. Yurkova; V. A. Zenin; G. S. Nagibina; B. S. Melnik; A. N. Fedorov
Source:
Applied biochemistry and microbiology 2019 v.55 no.6 pp. 588-595
ISSN:
0003-6838
Subject:
inclusion bodies,   etc   ; biosynthesis; hydrophobicity; recombinant proteins;     Show all 4 Subjects
Abstract:
... At biosynthetic production of hydrophobic recombinant proteins there is often a problem of their aggregation in so-called inclusion bodies, after which these proteins are difficult to renature. Obtaining such proteins in soluble forms is a formidable obstacle on the way of using them. One of possible solutions for this problem is the creation of fusion constructs with a leading protein. Previously ...
DOI:
10.1134/S0003683819130027

8. The impact of proteostasis dysfunction secondary to environmental and genetic causes on neurodegenerative diseases progression and potential therapeutic intervention

Author:
Abdelmagid M. Elmatboly; Ahmed M. Sherif; Dalia A. Deeb; Amira Benmelouka; May N. Bin-Jumah; Lotfi Aleya; Mohamed M. Abdel-Daim
Source:
Environmental science and pollution research international 2020 v.27 no.11 pp. 11461-11483
ISSN:
0944-1344
Subject:
inclusion bodies,   etc   ; Alzheimer disease; Parkinson disease; cell death; environmental factors; homeostasis; neurons; proteins; proteome; therapeutics;     Show all 10 Subjects
Abstract:
... Aggregation of particular proteins in the form of inclusion bodies or plaques followed by neuronal death is a hallmark of neurodegenerative proteopathies such as primary Parkinsonism, Alzheimer’s disease, Lou Gehrig’s disease, and Huntington’s chorea. Complex polygenic and environmental factors implicated in these proteopathies. Accumulation of proteins in these disorders indicates a substantial d ...
DOI:
10.1007/s11356-020-07914-1

9. Purification of viral neuraminidase from inclusion bodies produced by recombinant Escherichia coli

Author:
Sabina Lipničanová; Daniela Chmelová; Andrej Godány; Miroslav Ondrejovič; Stanislav Miertuš
Source:
Journal of biotechnology 2020 v.316 pp. 27-34
ISSN:
0168-1656
Subject:
inclusion bodies,   etc   ; Escherichia coli; Orthomyxoviridae; antiviral agents; arginine; enzymatic reactions; fetuins; glycerol; pH; sialidase; solubilization; urea; washing;     Show all 13 Subjects
Abstract:
... Neuraminidase (NA) is one of the targets for the development of new antivirals against the influenza virus. The recombinant Escherichia coli cells, namely the strains BL21(DE3)pLysS and ArcticExpress(DE3) were used to produce the influenza virus neuraminidase. Although the different conditions of induction were tested, the accumulation of over-expressed NA in insoluble fraction occurred independen ...
DOI:
10.1016/j.jbiotec.2020.04.005

10. Efficient expression of chondroitinase ABC I for specific disaccharides detection of chondroitin sulfate

Author:
Xingyu Lu; Qian Zhong; Jian Liu; Fulin Yang; Chenghui Lu; Huan Xiong; Sha Li; Yibo Zhu; Lingtian Wu
Source:
International journal of biological macromolecules 2020 v.143 pp. 41-48
ISSN:
0141-8130
Subject:
inclusion bodies,   etc   ; Escherichia coli; chondroitin sulfate; culture flasks; disaccharides; enzymes; fermenters; medicine; response surface methodology; signal peptide;     Show all 10 Subjects
Abstract:
... Chondroitinase ABC I (ChSase ABC I) is a key enzyme of chondroitin sulfate (CS) degradation and widely used for CS detection in the medicine filed. However, the recombinant ChSase ABC I was weakly expressed in Escherichia coli because the forms of it were mostly inclusion bodies. In this study, a signal peptide (pelB) was used for the soluble form expression of ChSase ABC I in E. coli. Then the cu ...
DOI:
10.1016/j.ijbiomac.2019.11.215

11. The Screening of Refolding Conditions and Obtainment of the Recombinant Antistaphylococcal Endolysin LysKCA in Active Form from E. coli Inclusion Bodies

Author:
A. V. Žydziecki; S. G. Golenchenko; U. A. Prakulevich; M. V. Sholukh
Source:
Applied biochemistry and microbiology 2020 v.56 no.1 pp. 44-50
ISSN:
0003-6838
Subject:
inclusion bodies,   etc   ; Escherichia coli; active sites; buffers; catalytic activity; endolysin; enzyme activity; pH; phosphates; redox potential; screening; sucrose; temperature;     Show all 13 Subjects
Abstract:
... Step-by-step screening of the main characteristics of refolding buffer is proposed to obtain recombinant antistaphylococcal endolysin LysK containing two catalytic domains, CHAP and amidase-2, in active form from E. coli inclusion bodies. The optimal pH, temperature, redox potential of the refolding buffer, as well as the optimal protein concentration and type of antiaggregation compound were dete ...
DOI:
10.1134/S0003683820010160

12. Detailed small-scale characterization and scale-up of active YFP inclusion body production with Escherichia coli induced by a tetrameric coiled coil domain

Author:
Robin Lamm; Vera D. Jäger; Benedikt Heyman; Christoph Berg; Christin Cürten; Ulrich Krauss; Karl-Erich Jaeger; Jochen Büchs
Source:
Journal of bioscience and bioengineering 2020 v.129 no.6 pp. 730-740
ISSN:
1389-1723
Subject:
inclusion bodies,   etc   ; Escherichia coli; acetates; batch fermentation; biomass; culture flasks; fluorescence; heterologous gene expression; monitoring; oxygen; pH; surface proteins; temperature;     Show all 13 Subjects
Abstract:
... During heterologous protein production with Escherichia coli, the formation of inclusion bodies (IBs) is often a major drawback as these aggregated proteins are usually inactive. However, different strategies for the generation of IBs consisting of catalytically active proteins have recently been described. In this study, the archaeal tetrameric coiled-coil domain of the cell-surface protein tetra ...
DOI:
10.1016/j.jbiosc.2020.02.003

13. Bacterial Inclusion Bodies: A Treasure Trove of Bioactive Proteins

Author:
Priyank Singhvi; Ankit Saneja; Sudeepa Srichandan; Amulya K. Panda
Source:
Trends in biotechnology 2020 v.38 no.5 pp. 474-486
ISSN:
0167-7799
Subject:
inclusion bodies,   etc   ; biotechnology; purification methods; recombinant proteins; solubilization; structure-activity relationships;     Show all 6 Subjects
Abstract:
... Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving tremendous attention for many diverse applications in the areas of industrial and medical biotechnology. Understanding the structure–function relationship of protein in IBs has recently created new possibilities in developing innovative isolation, solubilization, refolding, and purification processes for high-thro ...
DOI:
10.1016/j.tibtech.2019.12.011

14. Enhancement of piscine orthoreovirus-2 DNA vaccine potency by linkage of antigen gene to a trigger factor gene or signal peptide genes

Author:
Tomomasa Matsuyama; Tomokazu Takano; Miho Honjo; Takumi Kikuta; Akatsuki Nawata; Akira Kumagai; Ryo Honda; Takamitsu Sakai; Jun Kurita; Sachiko Terashima; Masatoshi Yamazaki; Yuta Matsuura; Yuko Nishizawa; Chihaya Nakayasu
Source:
Aquaculture 2021 v.533 pp. 736163
ISSN:
0044-8486
Subject:
inclusion bodies,   etc   ; Oncorhynchus kisutch; anemia; antibodies; antigens; antiserum; blood serum; cell lines; erythrocytes; etiological agents; fish; genes; genotype; muscles; necrosis; protective effect; recombinant vaccines; signal peptide; vaccination; vaccine development; viral growth; viral load; viral nonstructural proteins; virus replication; Japan;     Show all 25 Subjects
Abstract:
... Coho salmon (Oncorhynchus kisutch) is a Pacific salmon species that is commercially farmed in Japan, and is susceptible to erythrocyte inclusion body syndrome (EIBS), which is characterized by anemia, erythrocyte inclusion bodies, and cardiovascular muscle necrosis. At present, no vaccine for this disease is available. Piscine orthoreovirus-2 (PRV-2) is the causative agent of EIBS in Japan, and is ...
DOI:
10.1016/j.aquaculture.2020.736163

15. A novel Actinidia cytorhabdovirus characterized using genomic and viral protein interaction features

Author:
Yanxiang Wang; Guoping Wang; Jianyu Bai; Yongle Zhang; Ying Wang; Shaohua Wen; Liu Li; Zuokun Yang; Ni Hong
Source:
Molecular plant pathology 2021 v.22 no.10 pp. 1271-1287
ISSN:
1464-6722
Subject:
viral inclusion bodies,   etc   ; Actinidia chinensis; Cytorhabdovirus; Nicotiana benthamiana; fluorescence; genome; genomics; insect viruses; kiwifruit; microtubules; nucleotides; phylogeny; plant pathology; viruses; China;     Show all 15 Subjects
Abstract:
... A novel cytorhabdovirus, tentatively named Actinidia virus D (AcVD), was identified from kiwifruit (Actinidia chinensis) in China using high‐throughput sequencing technology. The genome of AcVD consists of 13,589 nucleotides and is organized into seven open reading frames (ORFs) in its antisense strand, coding for proteins in the order N‐P‐P3‐M‐G‐P6‐L. The ORFs were flanked by a 3′ leader sequence ...
DOI:
10.1111/mpp.13110

16. HBc-based virus-like particle assembly from inclusion bodies using 2-methyl-2, 4-pentanediol

Author:
Yao Zhang; Shuang Yin; Bingyang Zhang; Jingxiu Bi; Yongdong Liu; Zhiguo Su
Source:
Process biochemistry 2020 v.89 pp. 233-237
ISSN:
1359-5113
Subject:
inclusion bodies,   etc   ; Escherichia coli; hepatitis B antigens; protein subunits; recombinant fusion proteins; recombinant vaccines; sodium dodecyl sulfate; solubilization; surfactants; virus-like particles; viruses;     Show all 11 Subjects
Abstract:
... Hepatitis B virus core antigen (HBc) has recently been used as carriers to develop recombinant vaccines. However, not virus-like particles (VLPs) but inactive inclusion bodies are often formed for the chimeric proteins when expressed in Escherichia coli. A novel method for in vitro assembly of chimeric HBc-MAGE3 II from inclusion bodies to VLPs was established in this study. The method utilized 2- ...
DOI:
10.1016/j.procbio.2019.10.031

17. Induction of spawning in Pacific white shrimp Litopenaeus vannamei (Boone, 1931) by injection of its molt inhibiting hormone isoform II produced in E. coli

Author:
Mayte González; Jesús Luis Betancourt; Tania Rodríguez‐Ramos; Mario Pablo Estrada; Yamila Carpio; Laida Ramos
Source:
Aquaculture research 2020 v.51 no.8 pp. 3100-3108
ISSN:
1355-557X
Subject:
inclusion bodies,   etc   ; Escherichia coli; Litopenaeus vannamei; complementary DNA; females; genetic vectors; molting; neuropeptides; reverse transcriptase polymerase chain reaction; shrimp; shrimp culture; spawning; vitellogenesis;     Show all 13 Subjects
Abstract:
... The optimization of reproductive parameters in shrimp farming continues to be a challenge for most producing countries. Although the crustacean neuropeptides have been studied extensively in the last two decades, the functions of most of these neuropeptides remained putative. Among them, molt‐inhibiting hormone isoform II (MIH II) has shown an important role in vitellogenesis. In this study, the c ...
DOI:
10.1111/are.14644

18. More than just a phase: the search for membraneless organelles in the bacterial cytoplasm

Author:
Elio A. Abbondanzieri; Anne S. Meyer
Source:
Current genetics 2019 v.65 no.3 pp. 691-694
ISSN:
0172-8083
Subject:
inclusion bodies,   etc   ; bacteria; cell nucleolus; cytoplasmic granules; eukaryotic cells; granules; microstructure; organelles; ribonucleoproteins;     Show all 9 Subjects
Abstract:
... The bacterial cytoplasm, once thought to be a relatively undifferentiated reaction medium, has now been recognized to have extensive microstructure. This microstructure includes bacterial microcompartments, inclusion bodies, granules, and even some membrane-bound vesicles. Several recent papers suggest that bacteria may also organize their cytoplasm using an additional mechanism: phase-separated m ...
DOI:
10.1007/s00294-018-00927-x

19. Soluble recombinant pyruvate oxidase production in Escherichia coli can be enhanced and inclusion bodies minimized by avoiding pH stress

Author:
Jianguo Zhang; Junwen Lu; Erzheng Su
Source:
Journal of chemical technology and biotechnology 2019 v.94 no.8 pp. 2661-2670
ISSN:
0268-2575
Subject:
inclusion bodies,   etc   ; Escherichia coli; carbon; culture flasks; dissolved oxygen; glycerol; pH; pyruvate oxidase;     Show all 8 Subjects
Abstract:
... BACKGROUND: Recombinant pyruvate oxidase (pod) is in high demand owing to its broad application in biochemical analysis and low yield from wild microbial strains. Recombinant Escherichia coli harboring pET28a‐pod is an efficient producer of pod, facilitating its production in high yield. Optimizing cultivation using shake flasks is necessary for high‐level soluble pod production. RESULTS: During r ...
DOI:
10.1002/jctb.6075

20. Reverse transcription polymerase chain reaction (RT-PCR) detection for Australian Cherax reovirus from redclaw crayfish (Cherax quadricarinatus)

Author:
Orachun Hayakijkosol; Wansadaj Jaroenram; Leigh Owens; Jennifer Elliman
Source:
Aquaculture 2021 v.530 pp. 735881
ISSN:
0044-8486
Subject:
viral inclusion bodies,   etc   ; Cherax quadricarinatus; Reoviridae; amino acids; crayfish; cytoplasm; detection limit; diagnostic techniques; fish; genome; hosts; pathogens; reverse transcriptase polymerase chain reaction; shrimp;     Show all 14 Subjects
Abstract:
... Reoviruses have been isolated from many aquatic animals including fish and crustaceans. Viral inclusion bodies of reovirus have been found in the cytoplasm of the hepatopancreatic cells of redclaw crayfish (Cherax quadricarinatus). In the past, reverse transcription polymerase chain reaction (RT-PCR) designed from across other hosts was attempted to detect reovirus in redclaw crayfish but no speci ...
DOI:
10.1016/j.aquaculture.2020.735881