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1. Dry anaerobic digestion of organic waste: A review of operational parameters and their impact on process performance

Author:
Rocamora, Ildefonso; Wagland, Stuart T.; Villa, Raffaella; Simpson, Edmon W.; Fernández, Oliver; Bajón-Fernández, Yadira
Source:
Bioresource technology 2020 v.299 pp. 122681
ISSN:
0960-8524
Subject:
inoculum,   etc   ; anaerobic digestion; bulking agents; feedstocks; organic wastes; total solids;     Show all 6 Subjects
Abstract:
... Dry digestion is a suitable technology for treating organic wastes with varying composition such as the organic fraction of municipal solids waste. Yet, there is a need for further research to overcome some of the disadvantages associated with the high total solids content of the process. Optimisation of inoculum to substrate ratio, feedstock composition and size, liquid recirculation, bed compact ...
DOI:
10.1016/j.biortech.2019.122681

2. Removal of metronidazole from aqueous media by C. vulgaris

Author:
Hena, Sufia; Gutierrez, Leo; Croué, Jean-Philippe
Source:
Journal of hazardous materials 2020 v.384 pp. 121400
ISSN:
0304-3894
Subject:
inoculum,   etc   ; acute toxicity; adsorption; antibiotics; biomass production; metronidazole; zeta potential;     Show all 7 Subjects
Abstract:
... This current study investigated the removal of metronidazole from aqueous media by C. vulgaris. Two different initial sizes of inoculum (0.05 and 0.5 g L⁻¹) were tested for a wide concentration range of metronidazole (1–50 μM). The effect of metronidazole concentrations on biomass production was studied for 20 days. The exopolymeric substances (EPS) were quantified and correlated with the removal ...
DOI:
10.1016/j.jhazmat.2019.121400

3. Addition of electron shuttling compounds and different pH conditions for hydrogen production by a heat-treated sludge

Author:
Del Angel-Acosta, Yair A.; Alvarez, Luis H.; Garcia-Reyes, Refugio Bernardo; Garza-González, María Teresa; Carrillo-Reyes, Julián
Source:
Biocatalysis and agricultural biotechnology 2020 v.23 pp. 101507
ISSN:
1878-8181
Subject:
inoculum,   etc   ; fermentation; heat treatment; hydrogen production; oxidation; pH; sludge; sulfonates;     Show all 8 Subjects
Abstract:
... In the present work, the effect of anthraquinone-2-sulfonate (AQS) and its reduced form anthrahydroxyquinone-2-sulfonate (AH₂QS), and different pH values were evaluated during a dark fermentation process using heat-treated anaerobic granular sludge for hydrogen production. The highest maximum hydrogen production (1.99 mmol) was obtained by adding the reduced electron shuttling compound (AH₂QS) at ...
DOI:
10.1016/j.bcab.2020.101507

4. Conjugative transfer of Megaplasmids pND6–1 and pND6–2 enhancing naphthalene degradation in aqueous environment: characterization and bioaugmentation prospects

Author:
Wang, Shan; Li, Shanshan; Du, Dan; Wang, Dan; Yan, Wei
Source:
Applied microbiology and biotechnology 2020 v.104 no.2 pp. 861-871
ISSN:
0175-7598
Subject:
inoculum,   etc   ; Pseudomonas putida; bioaugmentation; genes; gentamicin; naphthalene; plasmids;     Show all 7 Subjects
Abstract:
... INTRODUCTION: Naphthalene catabolic strain Pseudomonas putida ND6 harbors two megaplasmids pND6–1 and pND6–2; naphthalene-degrading associated genes are located on pND6–1, while plasmid pND6–2 possesses potential coding genes for conjugative transfer. METHODS: In this study, the recombinant ND6 in which pND6–1 and pND6–2 were labeled with gentamicin and kanamycin-encoding genes, respectively, was ...
DOI:
10.1007/s00253-019-10273-8

5. Standardized protocol for determination of biohydrogen potential

Author:
Juárez, Oscar Aguilar; Carrillo-Reyes, Julián; Buitrón, Germán; Moreno-Andrade, Iván; Tapia-Rodríguez, Aida Cecilia; Palomo-Briones, Rodolfo; Razo-Flores, Elías; Aguilar-Juárez, Oscar; Arreola-Vargas, Jorge; Bernet, Nicolas; Braga, Adriana Ferreira Maluf; Braga, Lucia; Castelló, Elena; Chatellard, Lucile; Etchebehere, Claudia; Fuentes, Laura; León-Becerril, Elizabeth; Méndez-Acosta, Hugo Oscar; Ruiz-Filippi, Gonzalo; Tapia-Venegas, Estela; Trably, Eric; Wenzel, Jorge; Zaiat, Marcelo
Source:
MethodsX 2020 v.7 pp. 100754
ISSN:
2215-0161
Subject:
inoculum,   etc   ; biogas; biohydrogen; equipment; gas production (biological); hydrogen production; pH;     Show all 7 Subjects
Abstract:
... Biohydrogen production potential (BHP) depends on several factors like inoculum source, substrate, pH, among many others. Batch assays are the most common strategy to evaluate such parameters, where the comparison is a challenging task due to the different procedures used. The present method introduces the first internationally validated protocol, evaluated by 8 independent laboratories from 5 dif ...
DOI:
10.1016/j.mex.2019.11.027
PubMed:
32021817
PubMed Central:
PMC6993000

6. Elucidating the effect of microbial inoculum and ferric chloride as additives on the removal of antibiotic resistance genes from chicken manure during aerobic composting

Author:
Guo, Honghong; Gu, Jie; Wang, Xiaojuan; Nasir, Mubasher; Yu, Jing; Lei, Liusheng; Wang, Qianzhi
Source:
Bioresource technology 2020 v.309 pp. 122802
ISSN:
0960-8524
Subject:
inoculum,   etc   ; Firmicutes; additives; antibiotic resistance genes; composting; composts; ferric chloride; microbial activity; poultry manure; prediction;     Show all 10 Subjects
Abstract:
... This experiment investigated the effect of adding a microbial inoculum (M) and ferric chloride (F) on the fate of antibiotic resistance genes (ARGs) during chicken manure composting. Adding M and F improved the microbial activity in the compost and facilitated the removal of ARGs, whereas the combined treatment achieved the best results, especially in reducing the enrichment of sul resistance gene ...
DOI:
10.1016/j.biortech.2020.122802

7. Granular sludge is a preferable inoculum for the biochemical methane potential assay for two complex substrates

Author:
Posmanik, Roy; Kim, Andrew H.; Labatut, Rodrigo A.; Usack, Joseph G.; Angenent, Largus T.
Source:
Bioresource technology 2020 v.309 pp. 123359
ISSN:
0960-8524
Subject:
inoculum,   etc   ; acclimation; anaerobic digesters; biodegradability; biogas; biomass; methane; organic wastes; sludge;     Show all 9 Subjects
Abstract:
... The biochemical methane potential (BMP) assay is a standard method for characterizing biomethane potential and anaerobic biodegradability organic waste streams. Therefore, the BMP protocol must be standardized to reliably compare these parameters for various substrates. Here, the effect of inoculum selection on biomethane potential was investigated through BMP tests using two different substrates ...
DOI:
10.1016/j.biortech.2020.123359

8. Impact of non-thermal dielectric barrier discharge plasma on Staphylococcus aureus and Bacillus cereus and quality of dried blackmouth angler (Lophiomus setigerus)

Author:
Choi, Man-Seok; Jeon, Eun Bi; Kim, Ji Yoon; Choi, Eun Ha; Lim, Jun Sup; Choi, Jinsung; Park, Shin Young
Source:
Journal of food engineering 2020 v.278 pp. 109952
ISSN:
0260-8774
Subject:
inoculum,   etc   ; Bacillus cereus; Staphylococcus aureus; color; fillets; fisheries; flavor; food production; meat; pH; texture; thiobarbituric acid;     Show all 12 Subjects
Abstract:
... This study investigated the effects of dielectric barrier discharge (DBD) plasma against Staphylococcus aureus and Bacillus cereus (initial inoculum of 8–9 log₁₀ CFU/mL) on the surface of dried blackmouth angler, and the physicochemical [pH, Hunter colors, thiobarbituric acid reactive (TBA)] and sensorial quality on the dried blackmouth angler. Reductions in S. aureus and B. cereus counts on the f ...
DOI:
10.1016/j.jfoodeng.2020.109952

9. Innovative start-up strategies for single-stage deammonification with conventional activated sludge: Results of a pilot-scale demonstration

Author:
Choi, Daehee; Lee, Chulwoo; Jung, Jinyoung
Source:
Bioresource technology 2020 v.309 pp. 123423
ISSN:
0960-8524
Subject:
inoculum,   etc   ; activated sludge; anaerobic ammonium oxidation; batch systems; nitrifying bacteria; nitrogen;     Show all 6 Subjects
Abstract:
... This study investigated innovative start-up strategies of a pilot-scale sequencing batch reactor (SBR) for single-stage deammonification using activated sludge as the sole inoculum source. In 24 m³ aerobic oxidizing bacteria cultivation plant, nitrogen loss efficiency was suggested to be an indicator for determining duration of cultivation. In 12 m³ ANAMMOX (ANaerobic AMMonium OXidation) cultivati ...
DOI:
10.1016/j.biortech.2020.123423

10. Elemental sulfur-based autotrophic denitrification in stoichiometric S0/N ratio: Calibration and validation of a kinetic model

Author:
Huiliñir, C.; Acosta, L.; Yanez, D.; Montalvo, S.; Esposito, G.; Retamales, G.; Levicán, G.; Guerrero, L.
Source:
Bioresource technology 2020 v.307 pp. 123229
ISSN:
0960-8524
Subject:
inoculum,   etc   ; batch systems; denitrification; hydrolysis; kinetics; models; nitrate nitrogen; nitrates; poultry; stoichiometry; wastewater treatment;     Show all 11 Subjects
Abstract:
... The inclusion of S⁰ hydrolysis in a kinetic model of autotrophic denitrification has been recently proposed; however the model has not been calibrated or validated yet. Thus, a new methodology was developed and applied to calibrate and validate this kinetic model for the first time. An inoculum adapted from a poultry wastewater treatment plant at stoichiometric S⁰/NO₃⁻ ratio was used. The model wa ...
DOI:
10.1016/j.biortech.2020.123229

11. Continuous thermophilic hydrogen production from an enzymatic hydrolysate of agave bagasse: Inoculum origin, homoacetogenesis and microbial community analysis

Author:
Montiel-Corona, Virginia; Palomo-Briones, Rodolfo; Razo-Flores, Elías
Source:
Bioresource technology 2020 v.306 pp. 123087
ISSN:
0960-8524
Subject:
inoculum,   etc   ; acclimation; bagasse; hydrogen; hydrogen production; hydrolysates; lignocellulose; microbial communities; volatile fatty acids;     Show all 9 Subjects
Abstract:
... In this research, the performance of two thermophilic inocula of different origin on continuous hydrogen production from an enzymatic hydrolysate of agave bagasse were compared; one of them was obtained from a thermophilic reactor and the second one was taken from a mesophilic reactor and acclimated to thermophilic conditions. The acclimation process in one-step quickly established a high-performa ...
DOI:
10.1016/j.biortech.2020.123087

12. Optimization of the Ames RAMOS test allows for a reproducible high-throughput mutagenicity test

Author:
Kauffmann, Kira; Werner, Felix; Deitert, Alexander; Finklenburg, Julian; Brendt, Julia; Schiwy, Andreas; Hollert, Henner; Büchs, Jochen
Source:
The Science of the total environment 2020 v.717 pp. 137168
ISSN:
0048-9697
Subject:
inoculum,   etc   ; Ames test; auxotrophs; developmental stages; histidine; monitoring; mutagenicity; mutagens; temperature;     Show all 9 Subjects
Abstract:
... The Ames test is one of the most widely used mutagenicity tests. It employs histidine auxotrophic bacteria, which can mutate back to histidine prototrophy and, thus, grow on a histidine deficient medium. These mutants develop predominantly after adding a mutagenic compound during an initial growth phase on 1 mg/L histidine. In the established test systems, an endpoint determination is performed to ...
DOI:
10.1016/j.scitotenv.2020.137168

13. Preliminary evaluation of the anaerobic biodegradability of three biobased materials used for the production of disposable plastics

Author:
Calabro’, P.S.; Folino, A.; Fazzino, F.; Komilis, D.
Source:
Journal of hazardous materials 2020 v.390 pp. 121653
ISSN:
0304-3894
Subject:
inoculum,   etc   ; bags; biobased products; biodegradability; markets; methane; methane production; models; pollution; polyethylene; sodium hydroxide; temperature; wines;     Show all 13 Subjects
Abstract:
... Biodegradable plastics have been introduced to the market to substitute “traditional”, non-biodegradable, petro-based plastics to alleviate plastic pollution. Biochemical methane potential tests were carried out on compostable bags made of MaterBi®, biodegradable bottle wine corks and cellulosic plates to examine the anaerobic biodegradability of those materials. The impact of four factors: type o ...
DOI:
10.1016/j.jhazmat.2019.121653

14. Isolation, enhanced growth, and degradation characterization of a strain marine petroleum degrading bacteria

Author:
Yang, Yuping; Liu, Yingying; Li, Nana; Shi, Ke; Xue, Jianliang; Gao, Yu; Xiao, Xinfeng
Source:
Environmental technology & innovation 2020 v.18 pp. 100796
ISSN:
2352-1864
Subject:
inoculum,   etc   ; Halomonas; adsorption; bacteria; biodegradation; biomass; growth factors; pH; petroleum; salt concentration; variance;     Show all 11 Subjects
Abstract:
... In order to systematically explore the growth and biodegradation of marine petroleum degrading bacteria, the growth conditions of a strain marine petroleum degrading bacteria (HDMP2) were optimized. The degradation pathway of diesel by the bacteria was studied. The results showed that the strain of HDMP2 was Halomonas sp.nmyj-1. The main growth factors affecting HDMP2 were rotation rate, inoculum ...
DOI:
10.1016/j.eti.2020.100796

15. Techno-economic analysis for extracellular-polymeric substances (EPS) production using activated sludge fortified with crude glycerol as substrate and its application in leachate treatment

Author:
Kumar, Lalit R.; Zhang, Xiaolei; Kaur, Rajwinder; Yellapu, Sravan K.; Tyagi, R.D.; Drogui, Patrick
Source:
Bioresource technology 2020 v.303 pp. 122954
ISSN:
0960-8524
Subject:
inoculum,   etc   ; activated sludge; centrifugation; economic analysis; fermentation; fermenters; glycerol; leachates; prices;     Show all 9 Subjects
Abstract:
... Economic assessment of bio-flocculant production process has been carried out by SuperPro Designer where extracellular-polymeric substances (EPS) were produced using activated sludge fortified with crude glycerol in fermenter followed by centrifugation. Considering EPS concentration of 60 g/L in production fermenter at 96 h, the unit production cost for slime EPS was estimated to be $ 0.95/L. The ...
DOI:
10.1016/j.biortech.2020.122954

16. Validation of a screening method for the detection of colistin-resistant E. coli containing mcr-1 in feral swine feces

Author:
Chandler, Jeffrey C.; Franklin, Alan B.; Bevins, Sarah N.; Bentler, Kevin T.; Bonnedahl, Jonas; Ahlstrom, Christina A.; Bisha, Bledar; Shriner, Susan A.
Source:
Journal of microbiological methods 2020 v.172 pp. 105892
ISSN:
0167-7012
Subject:
inoculum,   etc   ; Escherichia coli; colistin; feces; feral animals; quantitative polymerase chain reaction; screening; swine; vancomycin;     Show all 9 Subjects
Abstract:
... A method was developed and validated for the detection of colistin-resistant Escherichia coli containing mcr-1 in the feces of feral swine. Following optimization of an enrichment method using EC broth supplemented with colistin (1 μg/mL) and vancomycin (8 μg/mL), aliquots derived from 100 feral swine fecal samples were spiked with of one of five different mcr-1 positive E. coli strains (between 1 ...
DOI:
10.1016/j.mimet.2020.105892

17. Dry batch anaerobic digestion of food waste in a box-type reactor system: Inoculum preparation and reactor performance

Author:
Rico, Carlos; Montes, Jesús A.; Lobo, Amaya
Source:
Journal of cleaner production 2020 v.251 pp. 119751
ISSN:
0959-6526
Subject:
inoculum,   etc   ; acclimation; anaerobic digestion; biogas; feedstocks; food waste; methane; methane production; slurries; volatile fatty acids;     Show all 10 Subjects
Abstract:
... A box-type reactor system with liquid inoculum has been studied for the dry anaerobic digestion of food waste. The food waste was processed without any pre-treatment to remove physical impurities, neither water addition to dilute and slurry the feedstock. The experiment was carried out with inoculum to substrate ratios of 1:1 (w/w) and 0.08:1 (VS basis). Previous acclimation of liquid inoculum enh ...
DOI:
10.1016/j.jclepro.2019.119751

18. Inactivation of Salmonella in cherry tomato stem scars and quality preservation by pulsed light treatment and antimicrobial wash

Author:
Leng, Juncai; Mukhopadhyay, Sudarsan; Sokorai, Kimberly; Ukuku, Dike O.; Fan, Xuetong; Olanya, Modesto; Juneja, Vijay
Source:
Food control 2020 v.110 pp. 107005
ISSN:
0956-7135
Subject:
inoculum,   etc   ; Salmonella enterica; Solanum lycopersicum; cherry tomatoes; firmness; pathogens; sanitizers; sensory properties; serotypes; texture;     Show all 10 Subjects
Abstract:
... The objective of this study was to evaluate the effectiveness of pulsed light (PL) treatment, a novel antimicrobial (LAPEN) wash and combinations thereof in inactivating Salmonella on stem scars of cherry tomato. The treatment effects on background microbiota and sensory quality was also investigated while in storage for 21 days at 10 °C. Three serotypes of Salmonella enterica were chosen to prepa ...
DOI:
10.1016/j.foodcont.2019.107005

19. Sugars altered fungal community composition and caused high network complexity in a Fusarium wilt pathogen-infested soil

Author:
Ren, Gaidi; Meng, Tianzhu; Ma, Yan
Source:
Biology and fertility of soils 2020 v.56 no.3 pp. 395-409
ISSN:
0178-2762
Subject:
inoculum,   etc   ; Fusarium wilt; added sugars; biomass; community structure; fungal communities; fungi; pathogens; root exudates; soil;     Show all 10 Subjects
Abstract:
... Despite the quantitative dominance of sugars within root exudates and their ecological importance in regulating plant disease development, it is not well understood how specific sugars influence the fate of fungal pathogens, the fungal community composition and, in particular, the fungal interactions in soil. In this study, a microcosm incubation experiment was conducted by adding four low-molecul ...
DOI:
10.1007/s00374-019-01424-0

20. Biodegradability and disintegration of multilayer starch films with electrospun PCL fibres encapsulating carvacrol

Author:
Tampau, Alina; González-Martínez, Chelo; Chiralt, Amparo
Source:
Polymer degradation and stability 2020 v.173 pp. 109100
ISSN:
0141-3910
Subject:
inoculum,   etc   ; biodegradability; biodegradation; carbon dioxide; carvacrol; composting; composts; encapsulation; lactones; starch; thermogravimetry; thermoplastics;     Show all 12 Subjects
Abstract:
... The biodegradation and disintegration of thermoplastic starch multilayers containing carvacrol(CA)-loaded poly-(ε-caprolactone) electrospun mats were evaluated under thermophilic composting conditions for 45 and 84 days, respectively, and compared with non-loaded carvacrol films and pure starch films. Sample mass loss, thermogravimetric and visual analyses were performed throughout the disintegrat ...
DOI:
10.1016/j.polymdegradstab.2020.109100