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1. Infectious in vitro transcripts from a cDNA clone of a Japanese gentian isolate of Sikte waterborne virus, which shows host-specific low-temperature-dependent replication

Author:
Fujisaki, Koki; Tateda, Chika; Abe, Yoshiko; Dominguez, John Jewish A.; Iwai, Mari; Obara, Kazue; Nakamura, Taiki; Iwadate, Yasuya; Kaido, Masanori; Mise, Kazuyuki
Source:
Archives of virology 2021 v.166 no.7 pp. 1991-1997
ISSN:
0304-8608
Subject:
inoculum,   etc   ; Tombusvirus; genomics; host specificity; virology; viruses;     Show all 6 Subjects
Abstract:
... Tombusviruses have been identified in several crops, including gentian virus A (GeVA) in Japanese gentian. In this study, we isolated another tombusvirus, Sikte waterborne virus strain C1 (SWBV-C1), from Japanese gentian. Although SWBV-C1 and GeVA are not closely related, SWBV-C1, like GeVA, showed host-specific low-temperature-dependent replication in gentian and arabidopsis. The use of in vitro ...
DOI:
10.1007/s00705-021-05074-2

2. A microbiome engineering framework to evaluate rhizobial symbionts of legumes

Author:
Quides, Kenjiro W.; Atamian, Hagop S.
Source:
Plant and soil 2021 v.463 no.1-2 pp. 631-642
ISSN:
0032-079X
Subject:
inoculum,   etc   ; biofertilizers; legumes; microbiome; soil; symbionts; symbiosis;     Show all 7 Subjects
Abstract:
... BACKGROUND: For well over a century, rhizobia have been recognized as effective biofertilizer options for legume crops. This has led to the widespread use of rhizobial inoculants in agricultural systems, but a recurring issue has emerged: applied rhizobia struggle to provide growth benefits to legume crops. This has largely been attributed to the presence of soil rhizobia and has been termed the ‘ ...
DOI:
10.1007/s11104-021-04892-2

3. Towards a Digital Bioprocess Replica: Computational Approaches in Biopharmaceutical Development and Manufacturing

Author:
Smiatek, Jens; Jung, Alexander; Bluhmki, Erich
Source:
Trends in biotechnology 2020 v.38 no.10 pp. 1141-1153
ISSN:
0167-7799
Subject:
inoculum,   etc   ; biopharmaceuticals; bioprocessing; biotechnology;     Show all 4 Subjects
Abstract:
... Quantitative unit operation models for the optimization and refinement of modern late-stage biopharmaceutical drug manufacturing processes have recently attracted increasing attention. The supplementary benefits of these models include increased process robustness and control in combination with a more stringent design of the bioprocess due to a reduced number of exploratory experiments. In additi ...
DOI:
10.1016/j.tibtech.2020.05.008

4. Quantitative resistance to witches’ broom disease in progenies of different sources of resistance

Author:
Pereira, Alezania Silva; Lopes, Uilson Vanderlei; Pires, José Luís; Barreto, Mariana Araujo; Pereira dos Santos, Lindolfo; Gonzaga, Renato Santos; Souza da Silva, Lorena; Jesus de Andrade, Franco; Solís Bonilla, José Luis; Gramacho, Karina Peres
Source:
Crop protection 2021 v.146 pp. 105678
ISSN:
0261-2194
Subject:
inoculum,   etc   ; Moniliophthora perniciosa; brooms; flowers; pathogens; plant protection; Brazil;     Show all 7 Subjects
Abstract:
... Genetic control using distinct resistant sources stands out as the best approach to control witches' broom disease (WBD) of cacao. Under a scenario of continuous and high inoculum pressure, in Bahia, Brazil, Moniliophthora perniciosa has evolved towards the Scavina's sources (the primary source of resistance used in cacao breeding programs). Therefore, the objectives of the present study were to m ...
DOI:
10.1016/j.cropro.2021.105678

5. CO Biomethanation with Different Anaerobic Granular Sludges

Author:
Zhang, Zhenwen; Ding, Chao; Wang, Luyang; Jiang, Bingyi; Söderlind, Ulf; Zhang, Wennan; Yu, Chunjiang
Source:
Waste and biomass valorization 2021 v.12 no.7 pp. 3913-3925
ISSN:
1877-2641
Subject:
inoculum,   etc   ; biogasification; biomass; juices; methane production; sludge; synthesis gas; temperature;     Show all 8 Subjects
Abstract:
... Biomethanation of the syngas from biomass gasification provides an alternative method for production of biofuel and chemicals. CO in syngas plays a key role in biomethanation of syngas, as it is both a substrate and an inhibitor of certain methanogenesis processes. In this study, CO biomethanation by using a mixture of N₂ and CO as the gas substrate, was investigated with the help of 5 adapted ana ...
DOI:
10.1007/s12649-020-01285-x

6. Low virus diversity and spread in wild Capsicum spp. accessions from Ecuador under natural inoculum pressure

Author:
Vélez-Olmedo, Jefferson B.; Quiñonez, Liliana Corozo; Vélez-Zambrano, Sergio M.; Monteros-Altamirano, Álvaro; De Oliveira, Athos S.; Resende, Renato O.
Source:
Archives of virology 2021 v.166 no.5 pp. 1447-1453
ISSN:
0304-8608
Subject:
inoculum,   etc   ; ; Capsicum; RNA; cultivars; pepper; virology; viruses; wild plants; Ecuador;     Show all 10 Subjects
Abstract:
... Challenging wild plant accessions with pathogens is an initial approach for finding resistance genes for breeding programs. Viruses can be transmitted artificially by mechanical or arthropod-borne inoculation, but these experimental assays do not always reproduce natural conditions in the field. In this study, 56 wild Capsicum spp. accessions from Ecuador that were under natural inoculum pressure ...
DOI:
10.1007/s00705-021-05027-9

7. Mass production of γ-Aminobutyric acid by semi-continuous fermentation using ceramic support by Lactobacillus brevis RK03

Author:
Hsueh, Yi-Huang; Yang, Jun-Huang; Ou, Shih-Fu; Chen, Shyi-Tien; Kuo, Jen-Min; Wu, Chien-Hui
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.140 pp. 110640
ISSN:
0023-6438
Subject:
inoculum,   etc   ; Lactobacillus brevis; ceramics; continuous fermentation; fermenters; monosodium glutamate; pH;     Show all 7 Subjects
Abstract:
... We evaluated γ-aminobutyric acid (GABA) production by Lactobacillus brevis RK03 immobilized fermentation using three types of porous ceramic materials, All Clean (AC), Ocean Free (OF), and Power Material (PM). Almost same surface areas of the three porous ceramic materials were used to perform batch cultures with a modified GM broth and 500 mM monosodium glutamate (MSG). PM produced the highest GA ...
DOI:
10.1016/j.lwt.2020.110640

8. Fitting of the In Vitro Gas Production Technique to the Study of High Concentrate Diets

Author:
Amanzougarene, Zahia; Fondevila, Manuel
Source:
Animals 2020 v.10 no.10
ISSN:
2076-2615
Subject:
inoculum,   etc   ; bicarbonates; fermentation; pH; saliva;     Show all 5 Subjects
Abstract:
... In vitro rumen fermentation systems are often adapted to forage feeding conditions, with pH values ranging in a range close to neutrality (between 6.5 and 7.0). Several attempts using different buffers have been made to control incubation pH in order to evaluate microbial fermentation under conditions simulating high concentrate feeding, but results have not been completely successful because of r ...
DOI:
10.3390/ani10101935

9. Anaerobic mono-digestion of sugarcane trash and bagasse with and without pretreatment

Author:
Paulose, Paulose; Kaparaju, Prasad
Source:
Industrial crops and products 2021 v.170 pp. 113712
ISSN:
0926-6690
Subject:
inoculum,   etc   ; catalysts; methane; methane production; stainless steel; steam; sugarcane bagasse; sugarcane trash; sulfuric acid;     Show all 9 Subjects
Abstract:
... The effect of organic loading on process performance and methane yields during anaerobic mono-digestion of sugarcane trash (ST) and sugarcane bagasse (SB) with and without dilute sulphuric acid impregnated steam explosion was investigated. At first, biochemical methane potential (BMP) of ST and SB was performed at 37 °C in batch assays with an inoculum to substrate (ISR) ratio of 2 on VS basis. Me ...
DOI:
10.1016/j.indcrop.2021.113712

10. Enhancing the in-situ enrichment of anammox bacteria in aerobic granules to achieve high-rate CANON at low temperatures

Author:
Liu, Wenru; Wang, Qian; Shen, Yaoliang; Yang, Dianhai
Source:
Chemosphere 2021 v.278 pp. 130395
ISSN:
0045-6535
Subject:
inoculum,   etc   ; Chloroflexi; ammonium; anaerobic ammonium oxidation; dissolved oxygen; nitrites; total nitrogen; wastewater; wastewater treatment;     Show all 9 Subjects
Abstract:
... In this study, a high-rate CANON (Complete Autotrophic Nitrogen-removal Over Nitrite) process was started up successfully by enhancing the in-situ enrichment of anammox bacteria in aerobic granules at conditions relevant for mainstream wastewater treatment. Firstly, to provide nitrite for anammox bacteria growth efficient nitrite-oxidizing bacteria (NOB) repression was rapidly achieved and stably ...
DOI:
10.1016/j.chemosphere.2021.130395

11. Scrap Iron Filings assisted nitrate and phosphate removal in low C/N waters using mixed microbial culture

Author:
Narayanasamydamodaran, Sanjena; Zuo, Jian’e; Ren, Haiteng; Kumar, Nawnit
Source:
Frontiers of environmental science & engineering 2021 v.15 no.4 pp. 66
ISSN:
2095-2201
Subject:
inoculum,   etc   ; adsorbents; byproducts; denitrification; dissolved oxygen; microbial culture; nitrate reduction; nitrates; phosphates;     Show all 9 Subjects
Abstract:
... This study focuses on identifying the factors under which mixed microbial seeds assist bio-chemical denitrification when Scrap Iron Filings (SIF) are used as electron donors and adsorbents in low C/N ratio waters. Batch studies were conducted in abiotic and biotic reactors containing fresh and aged SIF under different dissolved oxygen concentrations with [Formula: see text] and/or PO₄³⁻ influent(s ...
DOI:
10.1007/s11783-020-1358-2

12. Automation of high CHO cell density seed intensification via online control of the cell specific perfusion rate and its impact on the N-stage inoculum quality

Author:
Schulze, Markus; Lemke, Johannes; Pollard, David; Wijffels, Rene H.; Matuszczyk, Jens; Martens, Dirk E.
Source:
Journal of biotechnology 2021 v.335 pp. 65-75
ISSN:
0168-1656
Subject:
inoculum,   etc   ; apoptosis; automation; biomass; biotechnology; cell growth; cell viability; industry; physiological state;     Show all 9 Subjects
Abstract:
... Current CHO cell production processes require an optimized space-time-yield. Process intensification can support achieving this by enhancing the productivity and improving facility utilization. The use of perfusion at the last stage of the seed train (N-1) for high cell density inoculation of the fed-batch N-stage production culture is a relatively new approach with few industry applicable example ...
DOI:
10.1016/j.jbiotec.2021.06.011

13. Bioaugmentation strategy to enhance polycyclic aromatic hydrocarbons anaerobic biodegradation in contaminated soils

Author:
Ferraro, Alberto; Massini, Giulia; Miritana, Valentina Mazzurco; Panico, Antonio; Pontoni, Ludovico; Race, Marco; Rosa, Silvia; Signorini, Antonella; Fabbricino, Massimiliano; Pirozzi, Francesco
Source:
Chemosphere 2021 v.275 pp. 130091
ISSN:
0045-6535
Subject:
inoculum,   etc   ; anaerobic digestion; bioaugmentation; biodegradation; biomass; community structure; microbial communities; naphthalene; polycyclic aromatic hydrocarbons; wheat straw;     Show all 10 Subjects
Abstract:
... This paper proposes an innovative bioaugmentation approach for the remediation of polycyclic aromatic hydrocarbon (PAH) contaminated soils, based on a novel habitat-based strategy. This approach was tested using two inocula (i-24 and i-96) previously enriched through an anaerobic digestion process on wheat straw. It relies on the application of allochthonous microorganisms characterized by specifi ...
DOI:
10.1016/j.chemosphere.2021.130091

14. Consecutive alternating administration as an effective anti-coxsackievirus B3 in vivo treatment scheme

Author:
Stoyanova, Adelina; Galabov, Angel S.
Source:
Archives of virology 2021 v.166 no.7 pp. 1869-1875
ISSN:
0304-8608
Subject:
inoculum,   etc   ; antiviral agents; capsid; drug resistance; drug therapy; guanidines; neonates; progeny; virology; viruses;     Show all 10 Subjects
Abstract:
... Although chemotherapy is generally indicated for treatment of enterovirus infections, antivirals are currently not used in clinical practice. The use of monotherapy is the main reason for this unfavourable state. This is related to the fact that enterovirus progeny consist of innumerable quasispecies, allowing the virus to develop drug resistance quickly. Here, we present a consecutive alternating ...
DOI:
10.1007/s00705-021-05057-3

15. Optimal conditions for high solid co-digestion of organic fraction of municipal solid wastes in a leach-bed reactor

Author:
Nikulina, Nadiia; Uslu, Seda; Lemmer, Andreas; Azbar, Nuri; Oechsner, Hans
Source:
Bioresource technology 2021 v.331 pp. 125023
ISSN:
0960-8524
Subject:
inoculum,   etc   ; ammonia; anaerobic digestion; liquids; methane production; municipal solid waste; pH; poultry manure; temperature; Middle East;     Show all 10 Subjects
Abstract:
... Anaerobic co-digestion of organic fraction of municipal solid waste with solid content greater than 20% and chicken manure was investigated using leach-bed reactors in the framework of Middle East and North African countries. The objectives of the experiments were to determine the optimal ratio of organic fraction, chicken manure and solid inoculum, to compare temperature conditions and usage of l ...
DOI:
10.1016/j.biortech.2021.125023

16. Clonal resistance of rubber tree to Colletotrichum spp.

Author:
Antonio, Gabriel Leonardi; Scaloppi Junior, Erivaldo José; Fisher, Ivan Herman; Furtado, Edson Luis; Firmino, Ana Carolina
Source:
Forest pathology 2021 v.51 no.3 pp. e12685
ISSN:
1437-4781
Subject:
inoculum,   etc   ; Hevea brasiliensis; anthracnose; forests; fungi; gardens; leaf area; leaves; mycelium; rubber; Brazil;     Show all 11 Subjects
Abstract:
... The use of resistant clones of rubber trees could constitute a strategy for managing anthracnose disease caused by Colletotrichum. To examine this possibility, three isolates of Colletotrichum CH93 (C. fructicola) and CH 102 and CH 130 (C. acutatum) were used as inoculum for 20 rubber clones planted in Brazil. These evaluations took place in both a laboratory and in a clonal garden. For inoculatio ...
DOI:
10.1111/efp.12685

17. Development of an experimental model of septic knee arthritis in rats through intra-articular inoculation of Staphylococcus aureus

Author:
Achaerandio-de Nova, Ainara; Gómez-Juárez Sango, Mónica; Escudero-Jiménez, Ángel; Losa-Palacios, Sergio; Berenguer-Romero, María Dolores; Gerónimo-Pardo, Manuel
Source:
Laboratory animals 2021 v.55 no.3 pp. 270-280
ISSN:
1758-1117
Subject:
inoculum,   etc   ; Staphylococcus aureus; animal models; animal welfare; arthritis; bacteremia; histology; laboratories; synovial fluid;     Show all 9 Subjects
Abstract:
... Haematogenous models of septic arthritis have some inherent disadvantages, such as the manifestation of arthritis relies on chance, the size of the inoculum is unknown and the number of animals to be studied cannot be reduced because the animals cannot serve as their own controls. This study aimed to develop a rat model of knee septic arthritis by injecting a known inoculum of Staphylococcus aureu ...
DOI:
10.1177/0023677220978678

18. Equivalency of peroxyacetic acid to chlorine as a shell egg sanitizing rinse

Author:
Jones, Deana R.; Garcia, Javier S.; Gast, Richard K.; Ward, Garrett E.
Source:
Poultry science 2021 v.100 no.6 pp. 101069
ISSN:
0032-5791
Subject:
inoculum,   etc   ; Enterobacter cloacae; Salmonella Typhimurium; USDA; chlorine; eggs; indicator species; peracetic acid; poultry science; sanitation; sanitizers;     Show all 11 Subjects
Abstract:
... In the United States, all shell eggs processed under the USDA Agricultural Marketing Service voluntary grading standards must receive a shell sanitizing rinse of 100–200 ppm chlorine or its equivalent after leaving the washing process. A study was conducted to determine the concentration of peroxyacetic acid (PAA) which would be equivalent to 100–200 ppm chlorine (Cl) in reducing target organisms ...
DOI:
10.1016/j.psj.2021.101069

19. Field efficacy of Metarhizium acridum (Hypocreales: Clavicipitaceae) in the control of Bactrocera dorsalis (Diptera: Tephritidae) in citrus orchards in Senegal

Author:
Faye, Papa Djibril; Bal, Amadou Bocar; Ndiaye, Ndeye Meymouna; Diop, Fama; Sangaré, Yaye Konaré; Haddad, Charles; Coly, Emile Victor; Dieng, Elhadji Omar; Niassy, Saliou
Source:
International journal of tropical insect science 2021 v.41 no.2 pp. 1185-1195
ISSN:
1742-7592
Subject:
inoculum,   etc   ; ; Bactrocera dorsalis; Citrus; Metarhizium; insects; methyl eugenol; plant damage; Senegal;     Show all 9 Subjects
Abstract:
... The effectiveness of the “attract and kill” approach for the management of Bactrocera dorsalis in citrus orchards using autoinoculation strategy was evaluated in three locations in Senegal (Sindia, Sébikhotane and Ndoyene), between 2016 and 2018. Attractant Contaminant Traps (ACT) were treated with 0.3 g of Metarhizium acridum, and methyl eugenol was then deployed at densities of 25, 50 and 100 AC ...
DOI:
10.1007/s42690-020-00306-5

20. Hyperparasitism by Sphaerellopsis macroconidialis may lower over‐wintering survival of Uromycladium acaciae

Author:
Fraser, Stuart; McTaggart, Alistair R.; Roux, Jolanda; Wingfield, Mike J.
Source:
Forest pathology 2021 v.51 no.3 pp. e12691
ISSN:
1437-4781
Subject:
inoculum,   etc   ; Acacia mearnsii; Uromycladium; conidiomata; forests; hyperparasitism; internal transcribed spacers; phylogeny; population dynamics; South Africa;     Show all 10 Subjects
Abstract:
... Uromycladium acaciae has caused an epidemic of wattle rust on Acacia mearnsii in southern Africa since 2013. In April 2016, conidiomata of a species of Sphaerellopsis were observed parasitizing telia of U. acaciae on foliar samples collected from three plantations in Mpumalanga, South Africa. An isolate was identified as Sphaerellopsis macroconidialis based on a phylogenetic species hypothesis of ...
DOI:
10.1111/efp.12691