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... Fish cell lines are important tools in many biological studies. Here, we established a new Japanese flounder brain (JFB) cell line from the brain tissue of the Japanese flounder (Paralichthys olivaceus), which was successfully sub-cultured for over 120 passages, and consisted predominantly of fibroblast-like cells. Sequencing of the actin gene confirmed that JFB cells were derived from the Japanes ...
... A feeding experiment was carried out to compare the effects of dietary synthetic astaxanthin and Haematococcus pluvialis on growth, antioxidant capacity, immune response and hepato-morphology of Oncorhynchus mykiss under cage culture with flowing freshwater. Three isonitrogenous and isolipidic diets were formulated with or without synthetic astaxanthin and H. pluvialis and were administered to O. ...
interleukin-8, etc ; Ctenopharyngodon idella; Proteobacteria; community structure; exposure duration; fish; freshwater; homeostasis; human health; immune response; inflammation; interleukin-10; interleukin-6; intestinal microorganisms; intestinal mucosa; microbial communities; nanoplastics; oxidative stress; pollution; polystyrenes; toxicity; Show all 21 Subjects
Abstract:
... Nanoplastics (NPs) are emerging environmental contaminants with alarming ecological and human health concerns due to their unique physicochemical properties. This study elucidates the impacts of environmentally relevant concentrations (ERCs, 300 and 3000 ng/L) and sublethal concentrations (SLCs, 300 and 3000 μg/L) of polystyrene nanoplastics (PS-NPs, size 80 nm) on the intestinal immune response a ...
interleukin-8, etc ; Artemisia vulgaris; Oriental traditional medicine; acupuncture; asparagine; aspartic acid; blood; cell proliferation; glucose; interleukin-17; interleukin-23; medical treatment; metabolism; metabolites; metabolomics; models; nuclear magnetic resonance spectroscopy; psoriasis; taurine; triacylglycerols; valine; wool; Show all 22 Subjects
Abstract:
... Moxibustion is a traditional medical intervention of traditional Chinese medicine. It refers to the direct or indirect application of ignited moxa wool made of mugwort leaves to acupuncture points or other specific parts of the body for either treating or preventing diseases. Moxibustion has been proven to be effective in treating skin lesions of psoriasis. This study was performed to elucidate mo ...
... As a traditional medicine, seeds of Ginkgo biloba L. (Gbs) have been used to treat cough or asthma for a long time. It is commonly used in clinic for lung diseases. However, its mechanism of lung protection is not completely clear. This research was designed to explore the protective effects of Gbs on antioxidant and inflammation during the chronic obstructive pulmonary disease (COPD) pathological ...
interleukin-8, etc ; blood; humans; interleukin-4; macrophages; phosphorylation; Show all 6 Subjects
Abstract:
... JAK/STAT pathway plays a well-known role in macrophage polarization, but other signaling routes may also be involved. The aim of this study was to identify new signaling pathways and repolarize macrophages by selected protein kinase inhibitors. HL-60 derived macrophages were chosen as model cells and human blood macrophages were used for comparison. M1 and M2 polarization of HL60 derived and human ...
interleukin-8, etc ; Tachysurus fulvidraco; gene expression; innate immunity; liver; pathogens; Show all 6 Subjects
Abstract:
... Toll-Like Receptors (TLRs) are important pattern recognition receptors, playing critical roles in the early innate immune response to defensing against pathogen invasion. In this study, we found both soluble form TLR5 (pfTLR5S) and membrane form TLR5 (pfTLR5M) in yellow catfish Pelteobagrus fulvidraco. The open reading frames (ORFs) of pfTLR5M and pfTLR5S genes were 2655 bp and 1947 bp in length, ...
interleukin-8, etc ; antagonists; cholesterol; cryo-electron microscopy; crystal structure; neutrophils; Show all 6 Subjects
Abstract:
... The C‐X‐C motif chemokine CXCL8 (interleukin‐8, IL‐8) and its receptor chemokine receptor 2 (CXCR2) mediate neutrophil migration during cell development and inflammatory responses and thus are related to numerous inflammatory diseases and cancers. We have determined the cryo‐electron microscopy structure of CXCL8 bound CXCR2 coupled to Gᵢ protein, as well as the crystal structure of inactive CXCR2 ...
Snider Alexandria P.; Mccarthy Kacie L.; Wright-Johnson Elane C.; Ondrak Jeffrey D.; Oliver William T.; Dahlen Carl R.; Cushman Robert A.; Crouse Matthew Scott
interleukin-8, etc ; inflammation; seminal plasma; sperm concentration; spermatogenesis; spermatozoa; yearlings; Show all 7 Subjects
Abstract:
... We hypothesized that yearling bulls selected for a 28-d breeding season would have reduced sperm concentrations and morphology, and have increased seminal plasma concentrations of pro-inflammatory cytokine interleukin-8(IL-8). Yearling bulls were selected based on a breeding soundness examination (BSE) at approximately 415 d of age and contained at least 750 million sperm in the ejaculate, with 12 ...
interleukin-8, etc ; Andrographis paniculata; death; food science; intragastric administration; pharmacodynamics; rodents; therapeutics; toxicity; Show all 9 Subjects
Abstract:
... Andrographis paniculata (Burm.f.) Nees (AP) is widely used in most Asian and some Western countries. However, its main effects and underlying pharmacological mechanism have not been thoroughly characterized, and its safety has not been sufficiently investigated. The present study aimed to predict and visualize the potential targets and pathways, clarify the main pharmacological effects, and invest ...
interleukin-8, etc ; ambient temperature; analytical chemistry; biomarkers; cold storage; humans; shelf life; thermal stability; Show all 8 Subjects
Abstract:
... Lateral flow assays (LFAs) widely deployed for on-site diagnosis have predominantly utilized antibodies as recognition molecules. Antibodies with limited thermal stability deteriorate the performance of the LFA over time. Herein, we demonstrate a stable and robust LFA by utilizing thermally stable peptide-based 12–14 kDa affimers as recognition molecules, in lieu of conventional protein-based anti ...
interleukin-8, etc ; atopic dermatitis; dogs; ears; humans; hyperplasia; interleukin-10; mange; otitis externa; pathogenesis; Show all 10 Subjects
Abstract:
... Cutaneous cytokines and chemokines are involved in the pathogenesis of human and canine atopic dermatitis. The aim of the present study was to discriminate cytokine expression in the ear canals of atopic dogs with otitis, dogs with non-allergic inflammatory otitis (otodectic mange) and healthy non-atopic dogs. The ear canals of nine atopic dogs suffering from non-infected otitis externa (n = 14 ea ...
interleukin-8, etc ; DNA repair; acetylation; acetyltransferases; genes; lysine; methylation; phosphorylation; research; sirtuins; transcription (genetics); Show all 11 Subjects
Abstract:
... The non-POU domain-containing octamer-binding protein (NONO, also referred to as p54nrb) is a multifunctional nuclear protein engaging in transcriptional regulation, mRNA splicing, nuclear retention of defective RNA, and DNA repair. Emerging evidence has demonstrated that p54nrb is subjected to various posttranslational modifications, including phosphorylation and methylation, which may be importa ...
interleukin-8, etc ; DNA; fish; fluorescence; giant cells; hybridization; tetracycline; viral genome; virus replication; viruses; Show all 10 Subjects
Abstract:
... Visualization of the herpesvirus genomes during lytic replication and latency is mainly achieved by fluorescence in situ hybridization (FISH). Unfortunately, this technique cannot be used for the real-time detection of viral genome in living cells. To facilitate the visualization of the Marek’s disease virus (MDV) genome during all stages of the virus lifecycle, we took advantage of the well-estab ...
interleukin-8, etc ; citrulline; dose response; gene expression; hypothalamus; hypothermia; interleukin-10; neurons; nitric oxide; ultrastructure; Show all 10 Subjects
Abstract:
... The mechanism that mediates L-citrulline (L-Cit) hypothermia is poorly understood, and the involvement of nitric oxide signaling has not been fully elucidated. Therefore, this study aimed to determine L-Cit's influence on body temperature and to ascertain the central and peripheral mechanisms associated with this response. Chicks responded to intracerebroventricular (ICV) injection of L-Cit with h ...
Evi C. van Oostrom; Kiki EW Mulder; Marjolijn CE Verheul; Pauline A. Hendriksen; Suzan Thijssen; Aletta D. Kraneveld; Berber Vlieg-Boerstra; Johan Garssen; Joris C. Verster
interleukin-8, etc ; C-reactive protein; biomarkers; cross-sectional studies; healthy diet; immune system; questionnaires; saliva; Show all 8 Subjects
Abstract:
... The aim of this cross-sectional study was to investigate the association between a healthier diet, perceived immune fitness, and biomarkers of the immune system. N = 108 participants (31 men and 77 women), 18–30 years old, completed a questionnaire, comprising the Healthy Diet Scale (HDS) and a 1-item scale assessing perceived immune fitness. In addition, saliva samples were collected and C-reacti ...
interleukin-8, etc ; Escherichia coli; Western blotting; cortisol; endometrium; fluorescent antibody technique; inflammation; interleukin-6; lipopolysaccharides; phosphorylation; Show all 10 Subjects
Abstract:
... Bovine uterine infection is commonly caused by Escherichia coli (E. coli). Elevated concentrations of plasma cortisol have been reported in postpartum cows. However, the direct role of cortisol in the inflammatory response of bovine endometrial stromal cells (BESCs) remains unclear. Therefore, the aim of the study was to explore the regulatory effect of cortisol on lipopolysaccharide (LPS)-induced ...
interleukin-8, etc ; Western blotting; biotransformation; caffeic acid; inflammation; interleukin-1beta; phosphorylation; tryptophan; tumor necrosis factor-alpha; Show all 9 Subjects
Abstract:
... Currently, there is limited information available about cell-permeability and anti-cytokine activity of javamide-I/-II esters in monocyte/macrophage-like cells. Therefore, the aim of this study was to investigate their cell-permeability and anti-cytokine activity in the cells. The uptake of javamide-I/-II and esters was studied in THP-1 cells and PBMCs. Also, kinetic and inhibition studies were co ...
interleukin-8, etc ; Porcine epidemic diarrhea virus; apoptosis; diarrhea; dose response; industry; interleukin-6; intestines; microbiology; therapeutics; Show all 10 Subjects
Abstract:
... Porcine epidemic diarrhea virus (PEDV) is one of the main pathogens causing severe diarrhea in piglets. Infection of the host induces apoptosis, causing huge economic losses to the pig industry. At present, the preventive and therapeutic effects of commercial vaccines are not satisfactory, and it is necessary to develop new anti-PEDV drugs. In this study, we screened the PEDV-inhibiting drug Buddl ...
interleukin-8, etc ; chi-square distribution; interleukin-10; interleukin-6; meta-analysis; pancreatitis; risk; systematic review; therapeutics; Show all 9 Subjects
Abstract:
... It is widely accepted that inflammatory responses play a key role in acute pancreatitis (AP). We conducted a systematic review and meta-analysis to determine the effect of QingYi decoction on inflammatory markers.The PubMed, EMBASE, Cochrane, CNKI, CBM, and WANFGANG databases were searched for randomized controlled trials published before December 2019. Thirty-nine eligible studies were included i ...