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1. Biomimetic synthesis of galantamine via laccase/TEMPO mediated oxidative coupling

Author:
Zippilli, Claudio; Botta, Lorenzo; Bizzarri, Bruno Mattia; Baratto, Maria Camilla; Pogni, Rebecca; Saladino, Raffaele
Source:
RSC advances 2020 v.10 no.18 pp. 10897-10903
ISSN:
2046-2069
Subject:
laccase,   etc   ; biomimetic synthesis; ferricyanides;     Show all 3 Subjects
Abstract:
... Laccase-mediated intramolecular oxidative radical coupling of N-formyl-2-bromo-O-methylnorbelladine afforded a novel and isolable spirocyclohexadienonic intermediate of galantamine. High yield and conversion of substrate were obtained in the presence of the redox mediator 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). This laccase procedure, with an overall yield of 34%, represents a scalable and e ...
DOI:
10.1039/d0ra00935k

2. Recent development in the application of immobilized oxidative enzymes for bioremediation of hazardous micropollutants – A review

Author:
Shakerian, Farid; Zhao, Jing; Li, Shao-Ping
Source:
Chemosphere 2020 v.239 pp. 124716
ISSN:
0045-6535
Subject:
laccase,   etc   ; bioremediation; peroxidase; pollutants; prices;     Show all 5 Subjects
Abstract:
... During the past several years, abundant progresses has been made in the development of immobilized oxidative enzymes with focus on finding new support materials, improving the immobilization methods and their applications. Nowadays, immobilized oxidative enzymes are broadly accepted as a green way to face the challenge of high amounts of micropollutants in nature. Among all oxidative enzymes, lacc ...
DOI:
10.1016/j.chemosphere.2019.124716

3. Lectin-Modified Cryogels for Laccase Immobilization: a Decolorization Study

Author:
Bayraktaroğlu, Melis; Husein, İbrahim; Aktaş Uygun, Deniz; Uygun, Murat
Source:
Water, air, and soil pollution 2020 v.231 no.2 pp. 31
ISSN:
0049-6979
Subject:
laccase,   etc   ; concanavalin A; cryogels; decolorization; dyes;     Show all 5 Subjects
Abstract:
... In this presented work, poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [p(HEMA-GMA)] cryogels were prepared by using the radicalic cryopolymerization technique, and then modified with lectin protein ConA. These ConA functionalized cryogels were used for the immobilization of laccase, and enzyme loading was found to be as 7.1 mg/g cryogel. Finally, these laccase immobilized cryogels were s ...
DOI:
10.1007/s11270-020-4395-3

4. Reactivity of p-Coumaroyl Groups in Lignin upon Laccase and Laccase/HBT Treatments

Author:
Hilgers, Roelant; Kabel, Mirjam A.; Vincken, Jean-Paul
Source:
ACS sustainable chemistry & engineering 2020 v.8 no.23 pp. 8723-8731
ISSN:
2168-0485
Subject:
laccase,   etc   ; grasses; lignin; models; moieties; nuclear magnetic resonance spectroscopy; oxidation;     Show all 7 Subjects
Abstract:
... Laccase–mediator systems (LMS) are potential green tools for oxidative degradation and modification of lignin. Although LMS convert both phenolic and nonphenolic lignin structures, phenolic structures are more prone to react. Remarkably, in a previous study on laccase/HBT treatment of grasses, we observed the accumulation of p-coumaroyl moieties in residual lignin, even though such groups are free ...
DOI:
10.1021/acssuschemeng.0c02376

5. Beyond natural laccases: extension of their potential applications by protein engineering

Author:
Stanzione, Ilaria; Pezzella, Cinzia; Giardina, Paola; Sannia, Giovanni; Piscitelli, Alessandra
Source:
Applied microbiology and biotechnology 2020 v.104 no.3 pp. 915-924
ISSN:
0175-7598
Subject:
laccase,   etc   ; biocatalysts; industrial applications; mutagenesis; protein engineering; proteins; screening;     Show all 7 Subjects
Abstract:
... Laccases bring exciting promises into the green industries, and the development of enzymes with improved properties is further raising their exploitation potential. Molecular engineering methods to build highly efficient catalysts both through rational and random mutagenesis were extensively applied. Moreover, computational approaches are becoming always more reliable in aiding proper design of ef ...
DOI:
10.1007/s00253-019-10147-z

6. Laccase: addressing the ambivalence associated with the calculation of enzyme activity

Author:
Agrawal, Komal; Verma, Pradeep
Source:
3 Biotech 2019 v.9 no.10 pp. 365
ISSN:
2190-572X
Subject:
laccase,   etc   ; enzyme activity; guaiacol; oxygen;     Show all 4 Subjects
Abstract:
... Laccase (benzenediol: oxygen oxidoreductase) a unique multi-copper oxidase enzyme has been studied rigorously since its identification. However, there is ambivalence associated with various aspects of laccase, e.g., assay conditions and calculations. Our aim was to minimize its ambivalence, thus, total of five formulas (F1–F5) were used to determine laccase activity of white and blue laccase. In c ...
DOI:
10.1007/s13205-019-1895-1
PubMed:
31588389
PubMed Central:
PMC6754822

7. Can laccase-assisted processing conditions influence the structure of reaction products?

Author:
Su, Jing; Fu, Jiajia; Silva, Carla; Cavaco-Paulo, Artur
Source:
Trends in biotechnology 2019
ISSN:
0167-7799
Subject:
laccase,   etc   ; active sites; catechol; geometry;     Show all 4 Subjects
Abstract:
... Laccase is a promiscuous enzyme known to catalyse a wide range of phenolic substrates. Several poly(catechol) products can be obtained when processing modified enzymes or different reactors are used. The differentiated reaction products may be attributed to changes on the enzyme active site geometry induced by different environmental reaction settings. ...
DOI:
10.1016/j.tibtech.2019.03.006

8. Removal of trace organic contaminants by enzymatic membrane bioreactors: Role of membrane retention and biodegradation

Author:
Asif, Muhammad Bilal; Hou, Jingwei; Price, William E.; Chen, Vicki; Hai, Faisal I.
Source:
Journal of membrane science 2020 v.611 pp. 118345
ISSN:
0376-7388
Subject:
laccase,   etc   ; adsorption; artificial membranes; biodegradation; fouling; hydrophobicity; membrane bioreactors; nanofiltration; ultrafiltration;     Show all 9 Subjects
Abstract:
... Performance of an enzymatic membrane bioreactor (EMBR) equipped with either an ultrafiltration (UF) or a nanofiltration (NF) membrane was explored for the degradation of a set of 29 chemically diverse trace organic contaminants (TrOCs). The NF membrane provided effective retention (90-99%) of TrOCs within the NF-EMBR. On the other hand, partial retention of charged and significantly hydrophobic (l ...
DOI:
10.1016/j.memsci.2020.118345

9. Comparative evaluation of laccase mediated oxidized and unoxidized lignin of sugarcane bagasse for the synthesis of lignin-based formaldehyde resin

Author:
Raj, Akhil; Devendra, Leena P.; Sukumaran, Rajeev K.
Source:
Industrial crops and products 2020 v.150 pp. 112385
ISSN:
0926-6690
Subject:
laccase,   etc   ; acidolysis; alkaline hydrolysis; biorefining; formaldehyde; isolation techniques; lignin; lignocellulose; oxidation; physicochemical properties; sugarcane bagasse;     Show all 11 Subjects
Abstract:
... Pretreatment using dilute acid is one of the strategies employed in a lignocellulosic biorefinery. The current study deals with the isolation, characterization, and oxidation of lignin from the enzymatic residue of acid pre-treated sugarcane bagasse. The study also focused on evaluating the reactivity of Laccase mediated oxidized lignin with the non-oxidized one for the preparation of lignin-based ...
DOI:
10.1016/j.indcrop.2020.112385

10. An effective in-situ method for laccase immobilization: Excellent activity, effective antibiotic removal rate and low potential ecological risk for degradation products

Author:
Zhang, Chengyu; You, Shengping; Zhang, Jiaxing; Qi, Wei; Su, Rongxin; He, Zhimin
Source:
Bioresource technology 2020 v.308 pp. 123271
ISSN:
0960-8524
Subject:
laccase,   etc   ; Bacillus subtilis; Escherichia coli; ampicillin; antibacterial properties; biomineralization; ecotoxicology; risk; tetracycline;     Show all 9 Subjects
Abstract:
... In this study, we used a simple in-situ biomineralization method to immobilize Bacillus subtilis (B. subtilis)-derived laccase into the copper-Trimesic acid framework (Cu-BTC), and the synthesized Laccase@Cu-BTC particles were used to degrade tetracycline and ampicillin. Compared with free laccase, the Laccase@Cu-BTC showed 16.5-fold of activity recovery, higher thermo-tolerant performance, more e ...
DOI:
10.1016/j.biortech.2020.123271

11. Amperometric biosensor based on coupling aminated laccase to functionalized carbon nanotubes for phenolics detection

Author:
Othman, Abdelmageed M.; Wollenberger, Ulla
Source:
International journal of biological macromolecules 2020 v.153 pp. 855-864
ISSN:
0141-8130
Subject:
laccase,   etc   ; Coriolus; adsorption; biosensors; carbon electrodes; carbon nanotubes; catalytic activity; catechol; electrochemistry; storage time;     Show all 10 Subjects
Abstract:
... A biosensor for phenolic compounds based on a chemically modified laccase from Coriolus hirsuta immobilized on functionalized screen-printed carbon electrodes (SPCEs) was achieved. Different enzyme modifications and immobilization strategies were analyzed. The electrochemical response of the immobilized laccase on SPCEs modified with carboxyl functionalized multi-walled carbon nanotubes (COOH–MWCN ...
DOI:
10.1016/j.ijbiomac.2020.03.049

12. Laccases 2 & 3 as biomarkers of Botrytis cinerea infection in sweet white wines

Author:
Ployon, S.; Attina, A.; Vialaret, J.; Walker, A.S.; Hirtz, C.; Saucier, C.
Source:
Food chemistry 2020 v.315 pp. 126233
ISSN:
0308-8146
Subject:
laccase,   etc   ; Botrytis cinerea; biomarkers; food contamination; fungi; liquid chromatography; proteins; proteomics; small fruits; white wines;     Show all 10 Subjects
Abstract:
... Botrytized sweet wines are made with berries infected by the fungus Botrytis cinerea. The aim of this study was to identify biomarkers of B. cinerea infection in sweet wines with a focus on laccases which are exocellular oxidase enzymes produced by this fungus during fruit contamination. Total proteins from six commercial sweet wines, including three naturally botrytized wines and three non-botryt ...
DOI:
10.1016/j.foodchem.2020.126233

13. Lignin side chain region participates in Cd detoxification related to the cultivar-dependent Cd accumulation in Brassica chinensis L

Author:
Wang, Xue-song; Fu, Hui-ling; Gong, Fei-yue; Zhang, Yan; He, Chun-tao; Yang, Zhong-yi
Source:
Journal of hazardous materials 2020 v.392 pp. 122264
ISSN:
0304-3894
Subject:
laccase,   etc   ; Brassica rapa subsp. chinensis; cadmium; cell walls; cultivars; gene expression regulation; genes; lignin; lignin content;     Show all 9 Subjects
Abstract:
... To investigate the effect of lignin in the cultivar-dependent Cd detoxification of Brassica chinensis L., Cd and lignin contents, lignin composition and laccase genes expressions in low-Cd-accumulating (LAJK) and high-Cd-accumulating (HAJS) cultivars grown under control (CK) and 25 μM Cd-treatment were determined. The results showed that lignin combined about 14 % of total Cd in both LAJK and HAJS ...
DOI:
10.1016/j.jhazmat.2020.122264

14. ATP-responsive laccase@ZIF-90 as a signal amplification platform to achieve indirect highly sensitive online detection of ATP in rat brain

Author:
Zhang, Wang; Wang, Chao; Peng, Meihong; Ren, Guoyuan; Li, Kai; Lin, Yuqing
Source:
Chemical communications 2020 v.56 no.47 pp. 6436-6439
ISSN:
1364-548X
Subject:
laccase,   etc   ; adenosine triphosphate; brain; catalytic activity; chemical reactions; electrochemistry; encapsulation; enzyme activity; imidazole; monitoring; rats;     Show all 11 Subjects
Abstract:
... A novel electrochemical online system for indirect, highly sensitive and selective online monitoring of ATP in the cerebral microdialysate is presented based on the particular reaction of ATP with zeolitic imidazole framework-90 (ZIF-90) encapsulated laccase microcrystals (laccase@ZIF-90) and the natural catalytic activity of laccase. ...
DOI:
10.1039/d0cc02021d

15. Removal of bisphenol A in aqueous solution using magnetic cross-linked laccase aggregates from Trametes hirsuta

Author:
Sadeghzadeh, Sadegh; Ghobadi Nejad, Zahra; Ghasemi, Shahnaz; Khafaji, Mona; Borghei, Seyed Mehdi
Source:
Bioresource technology 2020 v.306 pp. 123169
ISSN:
0960-8524
Subject:
laccase,   etc   ; Coriolus hirsutus; aqueous solutions; bisphenol A; coprecipitation; crosslinking; immobilized enzymes; iron oxides; nanoparticles; pollutants;     Show all 10 Subjects
Abstract:
... Enzymatic removal of Bisphenol A (BPA), acknowledged as an environmentally friendly approach, is a promising method to deal with hard degradable contaminants. However, the application of “enzymatic treatment” has been limited due to lower operational stability and practical difficulties associated with recovery and recycling. Enzyme immobilization is an innovative approach which circumvents these ...
DOI:
10.1016/j.biortech.2020.123169

16. Development of a method to measure laccase activity on methoxyphenolic food ingredients and isomers

Author:
Manzano-Nicolas, Jesus; Marin-Iniesta, Fulgencio; Taboada-Rodriguez, Amaury; Garcia-Canovas, Francisco; Tudela-Serrano, Jose; Muñoz-Muñoz, Jose Luis
Source:
International journal of biological macromolecules 2020 v.151 pp. 1099-1107
ISSN:
0141-8130
Subject:
laccase,   etc   ; Coriolus versicolor; ascorbic acid; catalytic activity; enzyme activity; free radicals; guaiacol; ingredients; isomers; oxidation;     Show all 10 Subjects
Abstract:
... We studied the laccase-catalysed oxygenation of methoxyphenolic food ingredients, such as 2-methoxyphenol (guaiacol) and 2,6-dimethoxyphenol (syringol), isomers such as 3- and 4-methoxyphenol, and 2,3-, 3,4- and 3,5-dimethoxyphenol. These methoxyphenolic substrates generate unstable free radicals, which leads to the erroneous determination of steady state rates. The addition of small quantities of ...
DOI:
10.1016/j.ijbiomac.2019.10.152

17. Ciprofloxacin removal via sequential electro-oxidation and enzymatic oxidation

Author:
Cuprys, Agnieszka; Thomson, Paisley; Ouarda, Yassine; Suresh, Gayatri; Rouissi, Tarek; Kaur Brar, Satinder; Drogui, Patrick; Surampalli, Rao Y.
Source:
Journal of hazardous materials 2020 v.389 pp. 121890
ISSN:
0304-3894
Subject:
laccase,   etc   ; Coriolus versicolor; anodes; antibiotics; antimicrobial properties; ciprofloxacin; oxidation; oxides; wastewater;     Show all 9 Subjects
Abstract:
... The combination of electro-oxidation and enzymatic oxidation was tested to evaluate the potency of this system to remove ciprofloxacin (CIP), a fluoroquinolone antibiotic, from water. For the electro-oxidation boron-doped diamond (BDD) and mixed metal oxides anodes were tested, at three current densities (4.42, 17.7 and 35.4 A/cm²). BDD anode at 35.4 A/cm² exhibited the highest removal efficiency ...
DOI:
10.1016/j.jhazmat.2019.121890

18. Laccase-conjugated thiolated chitosan-Fe3O4 hybrid composite for biocatalytic degradation of organic dyes

Author:
Ulu, Ahmet; Birhanli, Emre; Boran, Filiz; Köytepe, Süleyman; Yesilada, Ozfer; Ateş, Burhan
Source:
International journal of biological macromolecules 2020 v.150 pp. 871-884
ISSN:
0141-8130
Subject:
laccase,   etc   ; catalytic activity; chitosan; coprecipitation; decolorization; dyes; enzyme stability; fabrics; iron oxides; magnetism; pH; temperature;     Show all 12 Subjects
Abstract:
... In this study, a novel immobilization support for laccase was developed to enhance enzyme stability, efficiency and reusability. Firstly, Fe₃O₄ magnetic particles were synthesized and modified by the co-precipitation route using thiolated chitosan (TCS). The support was characterized using several methods. Afterward, laccase was attached to the surface of functional support. The biochemical proper ...
DOI:
10.1016/j.ijbiomac.2020.02.006

19. Biodegradation of Tetracycline Antibiotic by Laccase Biocatalyst Immobilized on Chitosan-Tripolyphosphate Beads

Author:
Jeong, D.; Choi, K.-Y.
Source:
Applied biochemistry and microbiology 2020 v.56 no.3 pp. 306-312
ISSN:
0003-6838
Subject:
laccase,   etc   ; biocatalysts; biodegradation; chitosan; ferulic acid; metabolites; organic matter; tetracycline; thermal stability; triazoles; tripolyphosphates; vanillin;     Show all 12 Subjects
Abstract:
... In this study, biodegradation of tetracycline, which is one of the most commonly used antibiotics, was investigated. In order to convert tetracycline into inactive oxidative metabolites, laccase immobilized onto rigid tripolyphosphate-treated chitosan beads was utilized. For immobilization, chitosan surfaces were activated with glutaraldehyde-linker reagents after tripolyphosphate treatment. The g ...
DOI:
10.1134/S0003683820030047

20. Construction of the Heterologous Laccase Producer Aspergillus nidulans lac№4 (argB–) and Its Application for the Progesterone Transformation

Author:
Savinova, O. S.; Chulkin, A. M.; Savinova, T. S.; Vavilova, E. A.; Vasina, D. V.; Solyev, P. N.; Fedorova, T. V.
Source:
Applied biochemistry and microbiology 2020 v.56 no.3 pp. 321-328
ISSN:
0003-6838
Subject:
laccase,   etc   ; Aspergillus nidulans; Coriolus hirsutus; adsorption; biotransformation; catalytic activity; mycelium; progesterone; solid phase extraction; sorbents; steroids;     Show all 11 Subjects
Abstract:
... A novel Aspergillus nidulans lac№4 (argB–) strain, a producer of recombinant laccase A from basidiomycete Trametes hirsuta 072, was obtained. This strain was used for the biocatalytic transformation of progesterone (PG). The major biotransformation products are 11α-hydroxy-PG, 11α-acetoxy-PG and 6β, 11α-dihydroxy-PG. Macronet MN-200 sorbent was used to study the adsorption kinetics of PG, and the ...
DOI:
10.1134/S0003683820030114