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1. Enzymatic upgrading of heteroxylans for added-value chemicals and polymers

Author:
Thu V Vuong; Emma R Master
Source:
Current opinion in biotechnology 2022 v.73 pp. 51-60
ISSN:
0958-1669
Subject:
laccase,   etc   ; biotechnology; drugs; esterases; peroxidases; polymers; xylan;     Show all 7 Subjects
Abstract:
... Xylan is one of the most abundant, natural polysaccharides, and much recent interest focuses on upgrading heteroxylan to make use of its unique structures and chemistries. Significant progress has been made in the discovery and application of novel enzymes for debranching and modifying heteroxylans. Debranching enzymes include acetylxylan esterases, α-l-arabinofuranosidases and α-d-glucuronidases ...
DOI:
10.1016/j.copbio.2021.07.001

2. Preparation optimization and stability comparison study of alkali-modified biochar immobilized laccase under multi-immobilization methods

Author:
Zhaobo Wang; Dajun Ren; Hongyan Yu; Shuqin Zhang; Xiaoqing Zhang; Wangsheng Chen
Source:
Biochemical engineering journal 2022 v.181 pp. 108401
ISSN:
1369-703X
Subject:
laccase,   etc   ; adsorption; biochar; immobilized enzymes; pH; temperature;     Show all 6 Subjects
Abstract:
... Alkali modified biochar has attracted many interests as a potential enzyme immobilization carrier. In this paper, the preparation of alkali-modified immobilized laccases under multi-immobilization methods (adsorption: A/lac@A-MB; adsorption-crosslinking: A-C/lac@A-MB; covalent bonding: CB/lac@A-MB) were optimized via Design-Expert for the first time, and the stability of alkali-modified immobilize ...
DOI:
10.1016/j.bej.2022.108401

3. Genomic Organization of Streptomyces flavotricini NGL1 and Streptomyces erythrochromogenes HMS4 Reveals Differential Plant Beneficial Attributes and Laccase Production Capabilities

Author:
Richa Salwan; Randhir Kaur; Vivek Sharma
Source:
Molecular biotechnology 2022 v.64 no.4 pp. 447-462
ISSN:
1073-6085
Subject:
laccase,   etc   ; Streptomyces flavotricini; biotechnology; genomics; nucleotide sequences; remediation;     Show all 6 Subjects
Abstract:
... The genus Streptomyces has been explored in industrial sectors due to its endurance to environmental stresses, the production of a plethora of biomolecules, the biological remediation of soils, and alleviating plant stresses. The whole genome of NGL1 and HMS4 was sequenced due to the specific laccase activity against 2,6-dimethoxyphenol (2,6-DMP) and differential plant beneficial attributes. The d ...
DOI:
10.1007/s12033-021-00424-6

4. 3D printed PLA enzyme microreactors: Characterization and application for the modification of bioactive compounds

Author:
Elena Gkantzou; Anastasia Skonta; Aliki Tsakni; Angeliki Polydera; Dimitrios Moschovas; Konstantinos Spyrou; Apostolos Avgeropoulos; Dimitrios Gournis; Dimitra Houhoula; Haralambos Stamatis
Source:
Journal of biotechnology 2022 v.350 pp. 75-85
ISSN:
0168-1656
Subject:
laccase,   etc   ; Coriolus versicolor; biotechnology; biotransformation; chitosan; three-dimensional printing;     Show all 6 Subjects
Abstract:
... Process sustainability of biocatalytic processes is significantly empowered with the use of continuous-flow technologies that offer high productivity, minimal wastes and low volumetric consumption. Combining microreactor design with 3D printing technology can broaden the engineering potentials. This work proposes a protocol to modify the surface of 3D-printed PLA scaffolds, based on chitosan depos ...
DOI:
10.1016/j.jbiotec.2022.04.005

5. Superfine grinding pretreatment enhances emulsifying, gel properties and in vitro digestibility of laccase-treated α-Lactalbumin

Author:
Jialun Hu; Ling Ma; Xianqi Liu; Hongyu Li; Minghan Zhang; Zhanmei Jiang; Juncai Hou
Source:
Lebensmittel-Wissenschaft + Technologie 2022 v.157 pp. 113082
ISSN:
0023-6438
Subject:
laccase,   etc   ; crosslinking; gel strength; gels; hydrophobicity; intestines; molecular weight;     Show all 7 Subjects
Abstract:
... The main purpose of this study was to confirm the impact of superfine grinding treatment on physicochemical, functional properties and in vitro digestibility of laccase-treated α-Lactalbumin (α-Lac). With the grinding time ranging from 0 to 12 h, the high molecular weight polymers, relative dityrosine and surface hydrophobicity of laccase-treated α-Lac increased significantly (P ＜ 0.05). After sup ...
DOI:
10.1016/j.lwt.2022.113082

6. Photothermal catalytic degradation of textile dyes by laccase immobilized on Fe3O4@SiO2 nanoparticles

Author:
Qingpeng Zhu; Jiaojiao Song; Zhaofei Liu; Konglin Wu; Xiangzi Li; Zhiming Chen; Huan Pang
Source:
Journal of colloid and interface science 2022 pp. -
ISSN:
0021-9797
Subject:
laccase,   etc   ; alizarin; cost effectiveness; fabrics; lighting; malachite green; pH; wastewater treatment;     Show all 8 Subjects
Abstract:
... Efficient and convenient degradation of industrial organic pollutants are of great importance for green chemical schemes and cost-effectiveness. This work introduced the immobilization of laccase on Fe₃O₄@SiO₂ nanoparticles (Fe₃O₄@SiO₂-laccase) for photothermal catalytic degradation of textile dyes. The Fe₃O₄@SiO₂-laccase presented excellent reusability, improved thermal and pH stabilities, and re ...
DOI:
10.1016/j.jcis.2022.05.083

7. Conversion of lignin-derived 3-methoxycatechol to the natural product purpurogallin using bacterial P450 GcoAB and laccase CueO

Author:
Shun Zhang; Xiaofeng Wu; Yi Xiao
Source:
Applied microbiology and biotechnology 2022 v.106 no.2 pp. 593-603
ISSN:
0175-7598
Subject:
laccase,   etc   ; Escherichia coli; Quercus; antioxidants; biosynthesis; biotechnology; economic feasibility; lignin; microbiology; pyrogallol;     Show all 10 Subjects
Abstract:
... Purpurogallin is a natural benzotropolone extracted from Quercus spp, which has antioxidant, anticancer, and anti-inflammatory properties. Purpurogallin is typically synthesized from pyrogallol using enzymatic or metal catalysts, neither economically feasible nor environmentally friendly. 3-Methoxycatechol (3-MC) is a lignin-derived renewable chemical with the potential to be a substrate for the b ...
DOI:
10.1007/s00253-021-11738-5

8. Interactions between biofiller-modified polymeric composites and wood-rotting fungi in terms of their biotechnological applications

Author:
Beata Podkościelna; Anna Matuszewska; Dawid Stefaniuk; Marta Ruminowicz-Stefaniuk; Beata Ciołek; Magdalena Jaszek
Source:
Industrial crops and products 2022 pp. 115125
ISSN:
0926-6690
Subject:
laccase,   etc   ; Cerrena unicolor; Pycnoporus; biocompatible materials; bisphenol A; crosslinking; glass; lignin; polymerization; proteinases;     Show all 10 Subjects
Abstract:
... The synthesis and characterization of polymeric cross-linked composites derived from bisphenol A glycerolate diacrylate (BPA.GDA) and N-vinyl-2-2pyrrolidone (NVP) are presented. Commercially available kraft lignin with different weight % (0, 5, and 20) was applied as an environmentally friendly filler. The composites were made by bulk polymerization using a photoinitiator (2-dimethoxy-2-phenylacet ...
DOI:
10.1016/j.indcrop.2022.115125

9. Printing toner used as carrier for immobilization of laccase

Author:
Xijie Li; Juzheng Wang; Diyi Feng; Han Huang; Yuefeng Deng; Chengli Yang; Jie Ouyang; Dali Li; Ruofu Shi
Source:
Biocatalysis and agricultural biotechnology 2022 v.39 pp. 102272
ISSN:
1878-8181
Subject:
laccase,   etc   ; Agaricus bisporus; agricultural biotechnology; biocatalysis; chitosan; glutaraldehyde; immobilized enzymes; magnetism; pH; temperature;     Show all 10 Subjects
Abstract:
... The search for seeking novel immobilized enzyme carriers is a hot topic in the field of enzyme engineering. As a kind of common office consumable, magnetic printing toner has been terribly wasted, leading to potential harm to the environment. We try to use printing toner as carrier for enzyme immobilization to realize waste reuse. In this paper, the laccase extracted from Agaricus bisporus was suc ...
DOI:
10.1016/j.bcab.2021.102272

10. Decolorization and detoxification of synthetic dye compounds by laccase immobilized in vault nanoparticles

Author:
Yifan Gao; Meng Wang; Kshitija Shah; Shashank Singh Kalra; Leonard H. Rome; Shaily Mahendra
Source:
Bioresource technology 2022 v.351 pp. 127040
ISSN:
0960-8524
Subject:
laccase,   etc   ; acid orange 7; algae; anthraquinones; artificial colors; chlorophyll; decolorization; encapsulation; insects; pH; toxicity;     Show all 11 Subjects
Abstract:
... This study presents an eco-friendly and efficient technology, using immobilized enzymes, vault-encapsulated laccases (vlaccase), for decolorization and detoxification of dyes. Vault encapsulation remarkably improved the performance of laccase at industrially relevant conditions, including neutral to alkaline pH and relatively high temperatures. Two representative anthraquinone and azo dyes, Reacti ...
DOI:
10.1016/j.biortech.2022.127040

11. Impact of laccase-induced protein cross-linking on the in vitro starch digestion of black highland barley noodles

Author:
Jia-Xuan Fan; Xiao-Na Guo; Ke-Xue Zhu
Source:
Food hydrocolloids 2022 v.124 pp. 107298
ISSN:
0268-005X
Subject:
laccase,   etc   ; barley; barley flour; crosslinking; digestion; glycemic index; hydrocolloids; hydrolysis; noodles; sodium dodecyl sulfate;     Show all 10 Subjects
Abstract:
... The aim of this work is to evaluate the influence of laccase (LAC) on protein cross-linking and the in vitro starch digestion of black barley noodles (BHBN). In addition, the mechanisms underlying the impacts of protein cross-linking induced by LAC on the starch digestion were further elucidated. When the addition of LAC from 0% to 0.4%, in vitro starch digestion curves showed that the rate of in ...
DOI:
10.1016/j.foodhyd.2021.107298

12. Characterization of covalent, feruloylated polysaccharide gels by pulsed field gradient-stimulated echo (PFG-STE)-NMR

Author:
Florian Junker; Karin Michalski; Gisela Guthausen; Mirko Bunzel
Source:
Carbohydrate polymers 2021 v.267 pp. 118232
ISSN:
0144-8617
Subject:
laccase,   etc   ; glucose; viscoelasticity;     Show all 3 Subjects
Abstract:
... Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties. Here, the applicability of PFG-STE-NMR on feruloylated gels was proven, and relationships to rheological data were studied. Arabinoxylans and pectins were extracted from by-products using varying ...
DOI:
10.1016/j.carbpol.2021.118232

13. In silico and in vitro studies on the inhibition of laccase activity by Ellagic acid: Implications in drug designing for the treatment of Cryptococcal infections

Author:
Faizul Azam; Masood Alam Khan; Arif Khan; Shifa Ahmad; Syeda Fauzia Farheen Zofair; Hina Younus
Source:
International journal of biological macromolecules 2022 v.209 pp. 642-654
ISSN:
0141-8130
Subject:
laccase,   etc   ; Cryptococcus neoformans; computer simulation; drug resistance; drugs; ellagic acid; macrophages; molecular dynamics; thermodynamics; yeasts;     Show all 10 Subjects
Abstract:
... In recent years, the increased frequency of drug-resistant strains of Cryptococcus neoformans has depleted our antifungal armory. In the present study, we investigated the inhibitory potential of ellagic acid (EA) against C. neoformans laccase through in silico and in vitro studies. For the first time, a homology modelling was established to model laccase and modelled protein served as a receptor ...
DOI:
10.1016/j.ijbiomac.2022.04.060

14. The production of laccases by white-rot fungi under solid-state fermentation conditions

Author:
Daniela Chmelová; Barbora Legerská; Jana Kunstová; Miroslav Ondrejovič; Stanislav Miertuš
Source:
World journal of microbiology & biotechnology 2022 v.38 no.2 pp. 21
ISSN:
0959-3993
Subject:
laccase,   etc   ; bioreactors; biorefining; culture media; inoculum; lignocellulose; pollution; solid state fermentation; submerged fermentation; wastes; wood;     Show all 11 Subjects
Abstract:
... Laccases (E.C. 1.10.3.2) produced by white-rot fungi (WRF) can be widely used, but the high cost prevents their use in large-scale industrial processes. Finding a solution to the problem could involve laccase production by solid-state fermentation (SSF) simulating the natural growth conditions for WRF. SSF offers several advantages over conventional submerged fermentation (SmF), such as higher eff ...
DOI:
10.1007/s11274-021-03207-y

15. Biodegradation and Detoxification of Malachite Green Dye by Extracellular Laccase Expressed from Fusarium oxysporum

Author:
Le Thi Kim Thoa; Trinh Thi Phuong Thao; Nguyen Bao Hung; Kuan Shiong Khoo; Hoang Tan Quang; Tran Thuy Lan; Vu Duc Hoang; Seung-Moon Park; Chien Wei Ooi; Pau Loke Show; Nguyen Duc Huy
Source:
Waste and biomass valorization 2022 v.13 no.5 pp. 2511-2518
ISSN:
1877-2641
Subject:
laccase,   etc   ; Fusarium oxysporum; artificial colors; biodegradation; biomass; cations; enzymatic treatment; fungi; malachite green; temperature; toxicity; vanillin;     Show all 12 Subjects
Abstract:
... The present study evaluated the potential of biodegrading the toxic synthetic dye malachite green (MG) by an enzymatic approach. Extracellular laccase derived a fungal strain Fusarium oxysporum HUIB02 was used in the direct biodegradation of MG. Time-course study showed that the crude laccase successfully removed ≥ 80% MG after 20 h of treatment. The presence of Cu²⁺ ions enhanced MG degradation, ...
DOI:
10.1007/s12649-022-01692-2

16. Characterization of a pericarp browning related LACCASE 14-4 from longan fruit with a focus on (epi)catechin oxidative polymerization

Author:
Bin Liu; Qiunan Zhu; Xianfang Zhou; Xuelian Zhang; Zhaoxia Dang; Shuxing Liang; Ganting Li; Zhaoqi Zhang; Fang Fang; Xuequn Pang
Source:
Postharvest biology and technology 2022 v.185 pp. 111802
ISSN:
0925-5214
Subject:
laccase,   etc   ; amino acid sequences; catechin; catechol oxidase; endoplasmic reticulum; hybridization; longans; mesocarp; polymerization; procyanidins; technology; tobacco;     Show all 12 Subjects
Abstract:
... Polyphenol oxidase (PPO) has long been considered as the key enzyme responsible for the pericarp browning of longan fruit after harvest. However, due to the overlapping substrate ranges of PPOs and laccases, the contribution of laccases in longan pericarp browning is unclear. In this study, we found that the contents of flavan-3-ols and procyanidin A2/B2 in longan pericarp decreased during brownin ...
DOI:
10.1016/j.postharvbio.2021.111802

17. Combination strategy of laccase pretreatment and rhamnolipid addition enhance ethanol production in simultaneous saccharification and fermentation of corn stover

Author:
Huanan Li; Wenjing Xiao; Jiashu Liu; Qiuping Ran; Rui Wang; Cairong Yu; Xinyuan Zhang; Huiting Song; Zhengbing Jiang
Source:
Bioresource technology 2022 v.345 pp. 126414
ISSN:
0960-8524
Subject:
laccase,   etc   ; Saccharomyces cerevisiae; biosurfactants; corn stover; ethanol; ethanol production; fermentation; glucose; polysorbates; rhamnolipids; saccharification; saponins; tea;     Show all 13 Subjects
Abstract:
... The effects of laccase pretreatment and surfactant addition in the simultaneous saccharification and fermentation (SSF) of corn stover by engineered Saccharomyces cerevisiae were studied. Surfactants Tween-80, tea saponin and rhamnolipid improved ethanol production in SSF, among which the biosurfactant rhamnolipid reached the highest ethanol yield. At the 6 d in SSF, the ethanol content of additio ...
DOI:
10.1016/j.biortech.2021.126414

18. Improvement of laccase activity by silencing PacC in Ganoderma lucidum

Author:
Jing Zhu; Shuqi Song; Lindan Lian; Liang Shi; Ang Ren; Mingwen Zhao
Source:
World journal of microbiology & biotechnology 2022 v.38 no.2 pp. 32
ISSN:
0959-3993
Subject:
laccase,   etc   ; Ganoderma lucidum; acidification; biomass; biotechnology; fermentation; lignocellulose; nucleotide sequences; pH; signal peptide; white-rot fungi;     Show all 11 Subjects
Abstract:
... Ganoderma lucidum is a representative white-rot fungus that has great potential to degrade lignocellulose biomass. Laccase is recognized as a class of the most important lignin-degrading enzymes in G. lucidum. However, the comprehensive regulatory mechanisms of laccase are still lacking. Based on the genome sequence of G. lucidum, 15 laccase genes were identified and their encoding proteins were a ...
DOI:
10.1007/s11274-021-03216-x

19. Optimization of gluten-free bread production with low aflatoxin level based on quinoa flour containing xanthan gum and laccase enzyme

Author:
Ghodsieh Alizadeh-Bahaabadi; Leila Lakzadeh; Hamid Forootanfar; Hamid-Reza Akhavan
Source:
International journal of biological macromolecules 2022 v.200 pp. 61-76
ISSN:
0141-8130
Subject:
laccase,   etc   ; aflatoxin B1; cohesion; color; experimental design; gluten-free bread; hardness; quinoa flour; texture; xanthan gum;     Show all 10 Subjects
Abstract:
... This study was aimed to develop a new cereal-based product using quinoa flour, xanthan gum, and laccase and also to evaluate their effects on the quality characteristics of gluten-free bread (GFB). Experimental design method was applied for optimization of gluten-free formulation. The effects of three variables of quinoa flour (0–50%), laccase activity (0–2 U/g flour), and xanthan gum (0–0.5%) on ...
DOI:
10.1016/j.ijbiomac.2021.12.091

20. Potential of Halophilic Penicillium chrysogenum Isolated from Algerian Saline Soil to Produce Laccase on Olive Oil Wastes

Author:
Zeyneb Boucherit; Sigrid Flahaut; Brahim Djoudi; Toma-Nardjes Mouas; Aicha Mechakra; Souad Ameddah
Source:
Current microbiology 2022 v.79 no.6 pp. 178
ISSN:
0343-8651
Subject:
laccase,   etc   ; Penicillium chrysogenum; fungi; olive mill wastewater; olive oil; olive pomace; olives; pH; saline soils; tannase;     Show all 10 Subjects
Abstract:
... Enzymes from halophilic fungi offer interesting biotechnological applications, which lead us to explore novel producing strains. 23 fungi were isolated from Algerian saline soil. Among the three strains presenting laccase activities, one exhibited the high decolourising capacity of olive mill wastewaters. Identification showed that the efficient isolate GS15 belongs to Penicillium chrysogenum. Thi ...
DOI:
10.1007/s00284-022-02868-8