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laccase, etc ; biotechnology; drugs; esterases; peroxidases; polymers; xylan; Show all 7 Subjects
Abstract:
... Xylan is one of the most abundant, natural polysaccharides, and much recent interest focuses on upgrading heteroxylan to make use of its unique structures and chemistries. Significant progress has been made in the discovery and application of novel enzymes for debranching and modifying heteroxylans. Debranching enzymes include acetylxylan esterases, α-l-arabinofuranosidases and α-d-glucuronidases ...
laccase, etc ; adsorption; biochar; immobilized enzymes; pH; temperature; Show all 6 Subjects
Abstract:
... Alkali modified biochar has attracted many interests as a potential enzyme immobilization carrier. In this paper, the preparation of alkali-modified immobilized laccases under multi-immobilization methods (adsorption: A/lac@A-MB; adsorption-crosslinking: A-C/lac@A-MB; covalent bonding: CB/lac@A-MB) were optimized via Design-Expert for the first time, and the stability of alkali-modified immobilize ...
laccase, etc ; Streptomyces flavotricini; biotechnology; genomics; nucleotide sequences; remediation; Show all 6 Subjects
Abstract:
... The genus Streptomyces has been explored in industrial sectors due to its endurance to environmental stresses, the production of a plethora of biomolecules, the biological remediation of soils, and alleviating plant stresses. The whole genome of NGL1 and HMS4 was sequenced due to the specific laccase activity against 2,6-dimethoxyphenol (2,6-DMP) and differential plant beneficial attributes. The d ...
laccase, etc ; Coriolus versicolor; biotechnology; biotransformation; chitosan; three-dimensional printing; Show all 6 Subjects
Abstract:
... Process sustainability of biocatalytic processes is significantly empowered with the use of continuous-flow technologies that offer high productivity, minimal wastes and low volumetric consumption. Combining microreactor design with 3D printing technology can broaden the engineering potentials. This work proposes a protocol to modify the surface of 3D-printed PLA scaffolds, based on chitosan depos ...
laccase, etc ; crosslinking; gel strength; gels; hydrophobicity; intestines; molecular weight; Show all 7 Subjects
Abstract:
... The main purpose of this study was to confirm the impact of superfine grinding treatment on physicochemical, functional properties and in vitro digestibility of laccase-treated α-Lactalbumin (α-Lac). With the grinding time ranging from 0 to 12 h, the high molecular weight polymers, relative dityrosine and surface hydrophobicity of laccase-treated α-Lac increased significantly (P < 0.05). After sup ...
laccase, etc ; alizarin; cost effectiveness; fabrics; lighting; malachite green; pH; wastewater treatment; Show all 8 Subjects
Abstract:
... Efficient and convenient degradation of industrial organic pollutants are of great importance for green chemical schemes and cost-effectiveness. This work introduced the immobilization of laccase on Fe₃O₄@SiO₂ nanoparticles (Fe₃O₄@SiO₂-laccase) for photothermal catalytic degradation of textile dyes. The Fe₃O₄@SiO₂-laccase presented excellent reusability, improved thermal and pH stabilities, and re ...
laccase, etc ; Escherichia coli; Quercus; antioxidants; biosynthesis; biotechnology; economic feasibility; lignin; microbiology; pyrogallol; Show all 10 Subjects
Abstract:
... Purpurogallin is a natural benzotropolone extracted from Quercus spp, which has antioxidant, anticancer, and anti-inflammatory properties. Purpurogallin is typically synthesized from pyrogallol using enzymatic or metal catalysts, neither economically feasible nor environmentally friendly. 3-Methoxycatechol (3-MC) is a lignin-derived renewable chemical with the potential to be a substrate for the b ...
laccase, etc ; Cerrena unicolor; Pycnoporus; biocompatible materials; bisphenol A; crosslinking; glass; lignin; polymerization; proteinases; Show all 10 Subjects
Abstract:
... The synthesis and characterization of polymeric cross-linked composites derived from bisphenol A glycerolate diacrylate (BPA.GDA) and N-vinyl-2-2pyrrolidone (NVP) are presented. Commercially available kraft lignin with different weight % (0, 5, and 20) was applied as an environmentally friendly filler. The composites were made by bulk polymerization using a photoinitiator (2-dimethoxy-2-phenylacet ...
laccase, etc ; Agaricus bisporus; agricultural biotechnology; biocatalysis; chitosan; glutaraldehyde; immobilized enzymes; magnetism; pH; temperature; Show all 10 Subjects
Abstract:
... The search for seeking novel immobilized enzyme carriers is a hot topic in the field of enzyme engineering. As a kind of common office consumable, magnetic printing toner has been terribly wasted, leading to potential harm to the environment. We try to use printing toner as carrier for enzyme immobilization to realize waste reuse. In this paper, the laccase extracted from Agaricus bisporus was suc ...
laccase, etc ; acid orange 7; algae; anthraquinones; artificial colors; chlorophyll; decolorization; encapsulation; insects; pH; toxicity; Show all 11 Subjects
Abstract:
... This study presents an eco-friendly and efficient technology, using immobilized enzymes, vault-encapsulated laccases (vlaccase), for decolorization and detoxification of dyes. Vault encapsulation remarkably improved the performance of laccase at industrially relevant conditions, including neutral to alkaline pH and relatively high temperatures. Two representative anthraquinone and azo dyes, Reacti ...
laccase, etc ; barley; barley flour; crosslinking; digestion; glycemic index; hydrocolloids; hydrolysis; noodles; sodium dodecyl sulfate; Show all 10 Subjects
Abstract:
... The aim of this work is to evaluate the influence of laccase (LAC) on protein cross-linking and the in vitro starch digestion of black barley noodles (BHBN). In addition, the mechanisms underlying the impacts of protein cross-linking induced by LAC on the starch digestion were further elucidated. When the addition of LAC from 0% to 0.4%, in vitro starch digestion curves showed that the rate of in ...
... Translational mobility of guest molecules such as water and glucose in gels that are based on feruloylated polysaccharides appears to be critical to understand their nutritional and functional properties. Here, the applicability of PFG-STE-NMR on feruloylated gels was proven, and relationships to rheological data were studied. Arabinoxylans and pectins were extracted from by-products using varying ...
laccase, etc ; Cryptococcus neoformans; computer simulation; drug resistance; drugs; ellagic acid; macrophages; molecular dynamics; thermodynamics; yeasts; Show all 10 Subjects
Abstract:
... In recent years, the increased frequency of drug-resistant strains of Cryptococcus neoformans has depleted our antifungal armory. In the present study, we investigated the inhibitory potential of ellagic acid (EA) against C. neoformans laccase through in silico and in vitro studies. For the first time, a homology modelling was established to model laccase and modelled protein served as a receptor ...
laccase, etc ; bioreactors; biorefining; culture media; inoculum; lignocellulose; pollution; solid state fermentation; submerged fermentation; wastes; wood; Show all 11 Subjects
Abstract:
... Laccases (E.C. 1.10.3.2) produced by white-rot fungi (WRF) can be widely used, but the high cost prevents their use in large-scale industrial processes. Finding a solution to the problem could involve laccase production by solid-state fermentation (SSF) simulating the natural growth conditions for WRF. SSF offers several advantages over conventional submerged fermentation (SmF), such as higher eff ...
... The present study evaluated the potential of biodegrading the toxic synthetic dye malachite green (MG) by an enzymatic approach. Extracellular laccase derived a fungal strain Fusarium oxysporum HUIB02 was used in the direct biodegradation of MG. Time-course study showed that the crude laccase successfully removed ≥ 80% MG after 20 h of treatment. The presence of Cu²⁺ ions enhanced MG degradation, ...
... Polyphenol oxidase (PPO) has long been considered as the key enzyme responsible for the pericarp browning of longan fruit after harvest. However, due to the overlapping substrate ranges of PPOs and laccases, the contribution of laccases in longan pericarp browning is unclear. In this study, we found that the contents of flavan-3-ols and procyanidin A2/B2 in longan pericarp decreased during brownin ...
... The effects of laccase pretreatment and surfactant addition in the simultaneous saccharification and fermentation (SSF) of corn stover by engineered Saccharomyces cerevisiae were studied. Surfactants Tween-80, tea saponin and rhamnolipid improved ethanol production in SSF, among which the biosurfactant rhamnolipid reached the highest ethanol yield. At the 6 d in SSF, the ethanol content of additio ...
laccase, etc ; Ganoderma lucidum; acidification; biomass; biotechnology; fermentation; lignocellulose; nucleotide sequences; pH; signal peptide; white-rot fungi; Show all 11 Subjects
Abstract:
... Ganoderma lucidum is a representative white-rot fungus that has great potential to degrade lignocellulose biomass. Laccase is recognized as a class of the most important lignin-degrading enzymes in G. lucidum. However, the comprehensive regulatory mechanisms of laccase are still lacking. Based on the genome sequence of G. lucidum, 15 laccase genes were identified and their encoding proteins were a ...
... This study was aimed to develop a new cereal-based product using quinoa flour, xanthan gum, and laccase and also to evaluate their effects on the quality characteristics of gluten-free bread (GFB). Experimental design method was applied for optimization of gluten-free formulation. The effects of three variables of quinoa flour (0–50%), laccase activity (0–2 U/g flour), and xanthan gum (0–0.5%) on ...
... Enzymes from halophilic fungi offer interesting biotechnological applications, which lead us to explore novel producing strains. 23 fungi were isolated from Algerian saline soil. Among the three strains presenting laccase activities, one exhibited the high decolourising capacity of olive mill wastewaters. Identification showed that the efficient isolate GS15 belongs to Penicillium chrysogenum. Thi ...