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1. Single-cell isoform analysis in human immune cells

Author:
Roger Volden; Christopher Vollmers
Source:
Genome biology 2022 v.23 no.1 pp. 47
ISSN:
1474-760X
Subject:
B-lymphocytes; T-lymphocytes,   etc   ; genome; genomics; humans; immunologic receptors; monocytes; transcriptome;     Show all 8 Subjects
Abstract:
... High-throughput single-cell analysis today is facilitated by protocols like the 10X Genomics platform or Drop-Seq which generate cDNA pools in which the origin of a transcript is encoded at its 5′ or 3′ end. Here, we used R2C2 to sequence and demultiplex 12 million full-length cDNA molecules generated by the 10X Genomics platform from ~3000 peripheral blood mononuclear cells. We use these reads, i ...
DOI:
10.1186/s13059-022-02615-z

2. Designing an efficient epitope-based vaccine conjugated with a molecular adjuvant against Bovine Babesiosis: A computational study

Author:
Ali Forouharmehr; Narges Nazifi; Seyyed Mojtaba Mousavi; Amin Jaydari
Source:
Process biochemistry 2022 v.121 pp. 170-177
ISSN:
1359-5113
Subject:
B-lymphocytes; T-lymphocytes,   etc   ; Babesia; adjuvants; animals; babesiosis; databases; epitopes; molecular weight; nucleotide sequences; prediction; vaccines;     Show all 12 Subjects
Abstract:
... The current study was designed to introduce an efficient epitope-based vaccine against bovine babesiosis. Four immunogenic proteins of the Babesia. bovis including Trap, msa, bov57 and 12D3 were selected from VIOLIN database, then the proteins were used for prediction of B cell and T cell epitopes. The predicted epitopes along with HBHA protein as a molecular adjuvant were used to design an epitop ...
DOI:
10.1016/j.procbio.2022.06.016

3. Defects in aminoacyl-tRNA synthetase cause partial B and T cell immunodeficiency

Author:
Ju A. Shim; Yuna Jo; Hyunju Hwang; So Eun Lee; Dahyeon Ha; Jun Hwa Lee; Jayoung Kim; Parkyong Song; Dongjun Lee; Changwan Hong
Source:
Cellular and molecular life sciences 2022 v.79 no.2 pp. 87
ISSN:
1420-682X
Subject:
B-lymphocytes; T-lymphocytes,   etc   ; alanine-tRNA ligase; blood serum; cell viability; cytokines; immunosuppression; mutants; neurodegenerative diseases;     Show all 9 Subjects
Abstract:
... Aminoacyl-tRNA synthetases (ARSs) are emerging as important regulators in various immune diseases; however, their roles in immune cells remain unclear. In this study, using alanyl-tRNA synthetase (AARS) mutant (sti) mice with neurodegenerative disorder, we investigated the effect of translational fidelity in immune cells. Dysfunctional AARS caused disorders in immune cell responses and cellularity ...
DOI:
10.1007/s00018-021-04122-z

4. SILAC Phosphoproteomics Reveals Unique Signaling Circuits in CAR-T Cells and the Inhibition of B Cell-Activating Phosphorylation in Target Cells

Author:
Alijah A. Griffith; Kenneth P. Callahan; Nathan Gordo King; Qian Xiao; Xiaolei Su; Arthur R. Salomon
Source:
Journal of proteome research 2022 v.21 no.2 pp. 395-409
ISSN:
1535-3907
Subject:
B-lymphocytes; T-lymphocytes,   etc   ; antigens; coculture; liquid chromatography; phosphopeptides; phosphorylation; proteome; proteomics; research; tandem mass spectrometry;     Show all 11 Subjects
Abstract:
... Chimeric antigen receptor (CAR) is a single-pass transmembrane receptor designed to specifically target and eliminate cancers. While CARs prove highly efficacious against B cell malignancies, the intracellular signaling events which promote CAR T cell activity remain elusive. To gain further insight into both CAR T cell signaling and the potential signaling response of cells targeted by CAR, we an ...
DOI:
10.1021/acs.jproteome.1c00735

5. Discovery of a Transferrin Receptor 1-Binding Aptamer and Its Application in Cancer Cell Depletion for Adoptive T-Cell Therapy Manufacturing

Author:
Emmeline L. Cheng; Ian I. Cardle; Nataly Kacherovsky; Harsh Bansia; Tong Wang; Yunshi Zhou; Jai Raman; Albert Yen; Dominique Gutierrez; Stephen J. Salipante; Amédée des Georges; Michael C. Jensen; Suzie H. Pun
Source:
Journal of the American Chemical Society 2022 v.144 no.30 pp. 13851-13864
ISSN:
1520-5126
Subject:
B-lymphocytes; T-lymphocytes,   etc   ; DNA; antigens; genes; ligands; lymphoma; neoplasm cells; oligonucleotides; patients; therapeutics; transferrin receptors;     Show all 12 Subjects
Abstract:
... The clinical manufacturing of chimeric antigen receptor (CAR) T cells includes cell selection, activation, gene transduction, and expansion. While the method of T-cell selection varies across companies, current methods do not actively eliminate the cancer cells in the patient’s apheresis product from the healthy immune cells. Alarmingly, it has been found that transduction of a single leukemic B c ...
DOI:
10.1021/jacs.2c05349

6. The C-terminal domain of Bacillus cereus hemolysin II oligomerizes by itself in the presence of cell membranes to form ion channels

Author:
Natalia Rudenko; Alexander Siunov; Anna Zamyatina; Bogdan Melnik; Alexey Nagel; Anna Karatovskaya; Marina Borisova; Anna Shepelyakovskaya; Zhanna Andreeva-Kovalevskaya; Alexander Kolesnikov; Alexey Surin; Fedor Brovko; Alexander Solonin
Source:
International journal of biological macromolecules 2022 v.200 pp. 416-427
ISSN:
0141-8130
Subject:
B-lymphocytes; T-lymphocytes,   etc   ; Bacillus cereus; amino acid sequences; amino acids; erythrocytes; hemolysins; macrophages; monocytes; oligomerization; toxins;     Show all 11 Subjects
Abstract:
... Bacillus cereus hemolysin II, a pore-forming β-barrel toxin (HlyII), has a C-terminal extension of 94 amino acid residues, designated as the C-terminal domain of HlyII (HlyIICTD). HlyIICTD is capable of forming oligomers in aqueous solutions. Oligomerization of HlyIICTD significantly increased in the presence of erythrocytes and liposomes. Its affinity for erythrocytes of various origins differed ...
DOI:
10.1016/j.ijbiomac.2022.01.013

7. Dynamic imaging of chimeric antigen receptor t cells with [18f]tetrafluoroborate positron emission tomography/computed tomography

Author:
Sakemura Reona; Cox Michelle J.; Bansal Aditya; Roman Claudia Manriquez; Hefazi Mehrdad; Vernon Cynthia J.; Glynn Dianna L.; Pandey Mukesh K.; DeGrado Timothy R.; Siegler Elizabeth L.; Kenderian Saad S.
Source:
Journal of visualized experiments 2022 no.180 pp. e62334
ISSN:
1940-087X
Subject:
B-lymphocytes; T-lymphocytes,   etc   ; antigens; computed tomography; cytokines; iodides; myeloma; neurotoxicity; positron-emission tomography; sodium; symporters; therapeutics;     Show all 12 Subjects
Abstract:
... T cells genetically engineered to express chimeric antigen receptors (CAR) have shown unprecedented results in pivotal clinical trials for patients with B cell malignancies or multiple myeloma (MM). However, numerous obstacles limit the efficacy and prohibit the widespread use of CAR T cell therapies due to poor trafficking and infiltration into tumor sites as well as lack of persistence in vivo. ...
DOI:
10.3791/62334

8. Selective degradation of histone deacetylase 8 mediated by a proteolysis targeting chimera (PROTAC)

Author:
Jiranan Chotitumnavee; Yasunobu Yamashita; Yukari Takahashi; Yuri Takada; Tetsuya Iida; Makoto Oba; Yukihiro Itoh; Takayoshi Suzuki
Source:
Chemical communications 2022 v.58 no.29 pp. 4635-4638
ISSN:
1364-548X
Subject:
T-lymphocytes,   etc   ; histone deacetylase; leukemia; proteolysis;     Show all 4 Subjects
Abstract:
... We developed a first-in-class proteolysis targeting chimera (PROTAC) for selective degradation of histone deacetylase 8 (HDAC8). The PROTAC induced degradation of HDAC8 without affecting the levels of other HDACs in cellular assays, and inhibited the growth of T-cell leukemia Jurkat cells more potently than a conventional HDAC8 inhibitor. ...
DOI:
10.1039/d2cc00272h

9. Locally produced autoantibodies in cancer

Author:
Sara M. Centuori; Deepta Bhattacharya
Source:
Cell 2022 v.185 no.7 pp. 1110-1111
ISSN:
0092-8674
Subject:
B-lymphocytes,   etc   ; antibody formation; autoantibodies; metalloproteinases;     Show all 4 Subjects
Abstract:
... The function and antigen-specificities of tumor-infiltrating B cells are mostly unknown. In a new study by Mazor et al., matrix metalloproteinase 14 (MMP14), a self-antigen that is overexpressed by ovarian cancers, is shown to drive B cell activation and autoantibody production in tertiary lymphoid structures (Mazor et al., 2022). ...
DOI:
10.1016/j.cell.2022.03.006

10. A novel strategy to investigate the factors regulating the Treg to Tfr transition during acute viral infection

Author:
Huayu Lin; Haoqiang Wang; Qiao Liu; Zhiming Wang; Shuqiong Wen; Lisha Wang; Junyi Guo; Ling Ran; Zhengliang Yue; Qing Wu; Jianfang Tang; Zhirong Li; Li Hu; Lifan Xu; Qizhao Huang; Lilin Ye
Source:
Journal of immunological methods 2022 v.505 pp. 113266
ISSN:
0022-1759
Subject:
B-lymphocytes,   etc   ; chemokines; homeostasis; lymph nodes;     Show all 4 Subjects
Abstract:
... Follicular regulatory T cells (Tfrs), a specialized subset of regulatory T cells (Tregs), have a particular role in the control of follicular helper T cell-driven germinal center (GC) responses. Following differentiation signals similar to those received by follicular helper T cells (Tfhs), Tfrs gain expression of characteristic chemokine receptors and transcription factors, such as CXCR5 and Bcl- ...
DOI:
10.1016/j.jim.2022.113266

11. Current Understanding of the Role of T Cells in Chikungunya, Dengue and Zika Infections

Author:
Maheshi Mapalagamage; Daniela Weiskopf; Alessandro Sette; Aruna Dharshan De Silva
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
B-lymphocytes; CD8-positive T-lymphocytes,   etc   ; burden of disease; cross reaction; cytokines; dengue; epitopes; humans; lymph; protective effect; vaccines;     Show all 11 Subjects
Abstract:
... Arboviral infections such as Chikungunya (CHIKV), Dengue (DENV) and Zika (ZIKV) are a major disease burden in tropical and sub-tropical countries, and there are no effective vaccinations or therapeutic drugs available at this time. Understanding the role of the T cell response is very important when designing effective vaccines. Currently, comprehensive identification of T cell epitopes during a D ...
DOI:
10.3390/v14020242

12. Exploring the mechanism of berberine-mediated Tfh cell immunosuppression

Author:
Alexandra A. Vita; Nicholas A. Pullen
Source:
Phytomedicine 2022 v.105 pp. 154343
ISSN:
0944-7113
Subject:
B-lymphocytes; CD4-positive T-lymphocytes,   etc   ; arthritis; berberine; calcium; cell proliferation; dietary supplements; hypersensitivity; immunization; immunosuppression; interleukin-6; phosphorylation;     Show all 12 Subjects
Abstract:
... Our previous research revealed a novel function of berberine (BBR), a clinically relevant plant-derived alkaloid, as a suppressor of follicular T helper (Tfₕ) cell proliferation in secondary lymphoid organs of BBR-treated mice that underwent immunization for collagen-induced arthritis (CIA) in DBA1/J mice. Due to the importance of Tfₕ cell and B cell interactions in the generation of T cell-depend ...
DOI:
10.1016/j.phymed.2022.154343

13. TREC/KREC Levels and T and B Lymphocyte Subpopulations in COVID-19 Patients at Different Stages of the Disease

Author:
Andrei A. Savchenko; Elena Tikhonova; Igor Kudryavtsev; Dmitry Kudlay; Ilya Korsunsky; Vasily Beleniuk; Alexandr Borisov
Source:
Viruses 2022 v.14 no.3 pp. -
ISSN:
1999-4915
Subject:
B-lymphocytes; T-lymphocytes,   etc   ; COVID-19 infection; cell-mediated immunity; circular DNA; excision; flow cytometry; quantitative polymerase chain reaction;     Show all 8 Subjects
Abstract:
... Background: T and B cell-mediated immunity can be assessed using T cell receptor excision circle (TREC) and Kappa-deleting recombination excision circle (KREC) analysis, respectively, and successful implementation of this method requires evaluation of the correlation between the TREC frequencies and T cell subsets as well as KREC levels and B lymphocyte subsets. The aim of the present study was to ...
DOI:
10.3390/v14030646

14. CAR T cells are in it [CD]4 the long haul

Author:
Amanda C. Poholek
Source:
Cell 2022 v.185 no.7 pp. 1112-1114
ISSN:
0092-8674
Subject:
CD4-positive T-lymphocytes,   etc   ; cytotoxicity; patients; therapeutics;     Show all 4 Subjects
Abstract:
... In a recent issue of Nature, Melenhorst et al. perform an extensive analysis of CAR T cells that persist for ten years in CLL patients. They find a dominant cytotoxic CD4+ population, raising the possibility that CD4+ T cells play an important role in durable CAR T cell therapy. ...
DOI:
10.1016/j.cell.2022.03.004

15. Spatiotemporal behavior of T cells in vaccination

Author:
Ana Laura Chiodetti; Audrey Gérard
Source:
international journal of biochemistry & cell biology 2022 v.147 pp. 106224
ISSN:
1357-2725
Subject:
T-lymphocytes,   etc   ; biochemistry; cost effectiveness; vaccination; vaccines;     Show all 5 Subjects
Abstract:
... Vaccines are the most cost-effective resource to contain and eliminate infectious diseases. Despite decades of research in the field, several pathogens have eluded the effect of conventional vaccines mostly due their failure in inducing strong T cell responses. There is a need for new vaccine technologies that can surpass this problem. Recent advances in imaging techniques have allowed the study o ...
DOI:
10.1016/j.biocel.2022.106224

16. Characterization of the cell-mediated immune response to Takeda’s live-attenuated tetravalent dengue vaccine in adolescents participating in a phase 2 randomized controlled trial conducted in a dengue-endemic setting

Author:
Vianney Tricou; Raphael Gottardo; Michael A. Egan; Frédéric Clement; Geert Leroux-Roels; Xavier Sáez-Llorens; Astrid Borkowski; Derek Wallace; Hansi J. Dean
Source:
Vaccine 2022 v.40 no.8 pp. 1143-1151
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes,   etc   ; cell-mediated immunity; cross reaction; dengue; flow cytometry; interleukin-2; live vaccines; serotypes; Panama;     Show all 10 Subjects
Abstract:
... As robust dengue-specific CD4+ and CD8+ T cell responses are essential for protective immunity, we assessed cell-mediated immune (CMI) responses to a DENV-2-based dengue tetravalent vaccine candidate (TAK-003) in adolescents living in Panama, a dengue-endemic country. Peripheral blood mononuclear cells were collected from a subset of 67 participants ≥ 10 years old included in a phase 2 clinical tr ...
DOI:
10.1016/j.vaccine.2022.01.016

17. Humoral and cellular immune memory response 12 years following single dose vaccination against hepatitis A in Argentinian children

Author:
A. Urueña; M.N. Badano; P. Baré; J. González; R. Vicentín; R. Calli; M.C. Cañero-Velasco; S. Fink; C. Vizzotti
Source:
Vaccine 2022 v.40 no.1 pp. 114-121
ISSN:
0264-410X
Subject:
CD4-positive T-lymphocytes; CD8-positive T-lymphocytes,   etc   ; Hepatovirus A; antibodies; antigens; flow cytometry; hepatitis A; immunologic memory; memory; vaccination; vaccines; Argentina;     Show all 12 Subjects
Abstract:
... Infants’ universal hepatitis A virus (HAV) single-dose vaccination has been highly effective for controlling HAV infection in Argentina, and in other Latin-American countries that adopted that strategy. Although antibodies wane over time, this has not been associated with HAV outbreaks or breakthrough infections, suggesting a relevant role for memory immunity. This study assessed long term humoral ...
DOI:
10.1016/j.vaccine.2021.11.037

18. Localization of a TORC1-eIF4F translation complex during CD8+ T cell activation drives divergent cell fate

Author:
Swantje Liedmann; Xueyan Liu; Clifford S. Guy; Jeremy Chase Crawford; Diego A. Rodriguez; Duygu Kuzuoğlu-Öztürk; Ao Guo; Katherine C. Verbist; Jamshid Temirov; Mark J. Chen; Davide Ruggero; Hui Zhang; Paul G. Thomas; Douglas R. Green
Source:
Molecular cell 2022 v.82 no.13 pp. 2401-2414.e9
ISSN:
1097-2765
Subject:
CD8-positive T-lymphocytes,   etc   ; polyribosomes; synapse; transcription (genetics);     Show all 4 Subjects
Abstract:
... Activated CD8⁺ T lymphocytes differentiate into heterogeneous subsets. Using super-resolution imaging, we found that prior to the first division, dynein-dependent vesicular transport polarized active TORC1 toward the microtubule-organizing center (MTOC) at the proximal pole. This active TORC1 was physically associated with active eIF4F, required for the translation of c-myc mRNA. As a consequence, ...
DOI:
10.1016/j.molcel.2022.04.016

19. Non-viral siRNA delivery to T cells: Challenges and opportunities in cancer immunotherapy

Author:
Jelter Van Hoeck; Kevin Braeckmans; Stefaan C. De Smedt; Koen Raemdonck
Source:
Biomaterials 2022 v.286 pp. 121510
ISSN:
0142-9612
Subject:
T-lymphocytes,   etc   ; biocompatible materials; immunosuppression; immunotherapy; neoplasms; transfection;     Show all 6 Subjects
Abstract:
... T lymphocytes are the major drivers of antitumor immunity. The recent clinical success of adoptive T cell therapies and immune checkpoint inhibitors has demonstrated the strength of modulating T cell function in fighting cancer. Nonetheless, a significant fraction of patients remain unresponsive largely due to the immunosuppressive tumor environment that blunts T cell activity. Small interfering R ...
DOI:
10.1016/j.biomaterials.2022.121510

20. VSIG4/CRIg directly regulates early CD8+ T cell activation through its counter-receptor in a narrow window

Author:
Amrita Widyagarini; Naoto Nishii; Yohei Kawano; Chenyang Zhang; Miyuki Azuma
Source:
Biochemical and biophysical research communications 2022 v.614 pp. 100-106
ISSN:
0006-291X
Subject:
CD8-positive T-lymphocytes,   etc   ; homeostasis; ligands; phenotype; research;     Show all 5 Subjects
Abstract:
... T-cell responses are fine-tuned by positive and negative co-signal molecules expressed on immune cells and adjacent tissues. VSIG4 is a newly identified member of the B7 family of ligands, which negatively regulates innate inflammatory and CD4⁺ T cell-mediated responses. However, little is known about the direct effects of VSIG4, which are exerted through an unidentified counter-receptor on CD8⁺ T ...
DOI:
10.1016/j.bbrc.2022.04.120