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1. Fatty acid biosynthesis is enhanced in Escherichia coli strains with deletion in genes encoding the PII signaling proteins

Author:
Rodrigues, Thiago Estefano; Sassaki, Guilherme Lanzi; Valdameri, Gláucio; Pedrosa, Fábio Oliveira; Souza, Emanuel Maltempi; Huergo, Luciano Fernandes
Source:
Archives of microbiology 2019 v.201 no.2 pp. 209-214
ISSN:
0302-8933
Subject:
malonyl coenzyme A,   etc   ; Escherichia coli; acetyl-CoA carboxylase; bacteria; biosynthesis; fatty acids; fuel production; genes; models; prokaryotic cells; proteins;     Show all 11 Subjects
Abstract:
... The committed and rate-limiting step in fatty acid biosynthesis is catalyzed by acetyl-CoA carboxylase (ACC). In previous studies we showed that ACC activity is inhibited through interactions with the PII signaling proteins in vitro. Here we provide in vivo support for that model; we noted that PII proteins are able to reduce malonyl-CoA levels in vivo in Escherichia coli. Furthermore, we show tha ...
DOI:
10.1007/s00203-018-1603-2

2. Enhancement of acetyl-CoA flux for photosynthetic chemical production by pyruvate dehydrogenase complex overexpression in Synechococcus elongatus PCC 7942

Author:
Hirokawa, Yasutaka; Kubo, Takeshi; Soma, Yuki; Saruta, Fumiko; Hanai, Taizo
Source:
Metabolic engineering 2020 v.57 pp. 23-30
ISSN:
1096-7176
Subject:
malonyl coenzyme A,   etc   ; NAD (coenzyme); Synechococcus sp. PCC 7942; acetates; acetone; acetyl coenzyme A; biochemical pathways; carbon dioxide; catalytic activity; decarboxylation; enzyme activity; fatty acids; gene overexpression; genes; genetic engineering; isoprene; isopropyl alcohol; metabolites; models; photosynthesis; pyruvate dehydrogenase (lipoamide); pyruvic acid;     Show all 22 Subjects
Abstract:
... Genetic manipulation in cyanobacteria enables the direct production of valuable chemicals from carbon dioxide. However, there are still very few reports of the production of highly effective photosynthetic chemicals. Several synthetic metabolic pathways (e.g., isopropanol, acetone, isoprene, and fatty acids) have been constructed by branching from acetyl-CoA and malonyl-CoA, which are key intermed ...
DOI:
10.1016/j.ymben.2019.07.012

3. Resource partitioning strategies during toxin production in Microcystis aeruginosa revealed by integrative omics analysis

Author:
Pan, Di; Pavagadhi, Shruti; Umashankar, Shivshankar; Rai, Amit; Benke, Peter I.; Rai, Megha; Saxena, Gourvendu; Gangu, Vamshidhar; Swarup, Sanjay
Source:
Algal research 2019 v.42 pp. 101582
ISSN:
2211-9264
Subject:
malonyl coenzyme A,   etc   ; Microcystis aeruginosa; algae; algal blooms; cell growth; climate change; metabolome; microcystins; mutants; nitrogen; resource allocation; secondary metabolites; statistical models; toxicity; transcriptome; urbanization;     Show all 16 Subjects
Abstract:
... Escalating rates of urbanization and climate change have led to increased occurrence of harmful algal blooms (HABs) in natural waters. HABs caused by Microcystis aeruginosa produce toxic secondary metabolites, such as microcystins (MCs). To understand resource partitioning strategies between cell growth and MC production in response to environmental triggers, we tested the effect of interactions b ...
DOI:
10.1016/j.algal.2019.101582

4. Metabolic engineering of Saccharomyces cerevisiae for efficient production of endocrocin and emodin

Author:
Sun, Lei; Liu, Guiyou; Li, Ya; Jiang, Dayong; Guo, Wenfeng; Xu, Hui; Zhan, Ruoting
Source:
Metabolic engineering 2019 v.54 pp. 212-221
ISSN:
1096-7176
Subject:
malonyl coenzyme A,   etc   ; Saccharomyces cerevisiae; acetyl-CoA carboxylase; batch fermentation; biochemical pathways; biosynthesis; bricks; carbon; catalytic activity; emodin; metabolic engineering; mutants; screening;     Show all 13 Subjects
Abstract:
... The anthraquinones endocrocin and emodin are synthesized by a special class of type I NR-PKSs and a discrete MβL-TE. In this work, we first reconstituted a biosynthetic pathway of endocrocin and emodin in S. cerevisiae by combining enzymes from different sources. We functionally characterized a TE-less NR-PKS (SlACAS) and a MβL-TE (SlTE) from S. lycopersici as well as four orthologous MβL-TEs. SlA ...
DOI:
10.1016/j.ymben.2019.04.008

5. Capillary electrophoretic assay of human acetyl‐coenzyme A carboxylase 2

Author:
Nguyen, Thu H.; Waldrop, Grover L.; Gilman, S. Douglass
Source:
Electrophoresis 2019 v.40 no.11 pp. 1558-1564
ISSN:
0173-0835
Subject:
malonyl coenzyme A,   etc   ; acetyl coenzyme A; acetyl-CoA carboxylase; capillary electrophoresis; carboxylation; catalytic activity; citrates; magnesium; magnesium chloride; pH; potassium; potassium bicarbonate;     Show all 12 Subjects
Abstract:
... Human acetyl‐coenzyme A carboxylase 2 catalyzes the carboxylation of acetyl coenzyme A to form malonyl coenzyme A, along with the conversion of magnesium‐adenosine triphosphate complex to magnesium‐adenosine diphosphate complex. A simple off‐column capillary electrophoresis assay for human acetyl‐coenzyme A carboxylase 2 was developed based on the separation of magnesium‐adenosine triphosphate com ...
DOI:
10.1002/elps.201800514

6. Storage carbon metabolism of Isochrysis zhangjiangensis under different light intensities and its application for co-production of fucoxanthin and stearidonic acid

Author:
Li, Yuelian; Sun, Han; Wu, Tao; Fu, Yunlei; He, Yongjin; Mao, Xuemei; Chen, Feng
Source:
Bioresource technology 2019 v.282 pp. 94-102
ISSN:
0960-8524
Subject:
malonyl coenzyme A,   etc   ; Isochrysis; alpha-ketoglutaric acid; carbon metabolism; carotenoids; cell growth; citrates; glyceraldehyde 3-phosphate; light intensity; lipid content; lipid metabolism;     Show all 11 Subjects
Abstract:
... This study explored the co-production of fucoxanthin and stearidonic acid from Isochrysis zhangjiangensis by investigating its carbon metabolism under different light intensities. Results showed high light inhibited the synthesis of fucoxanthin and stearidonic acid, while promoted cell growth and enhanced cellular lipid content compared with low light, achieving 2.4 g/L and 28.55%, respectively. L ...
DOI:
10.1016/j.biortech.2019.02.127

7. Modeling transcriptional factor cross-talk to understand parabolic kinetics, bimodal gene expression and retroactivity in biosensor design

Author:
Aris, Hannah; Borhani, Shayan; Cahn, Devorah; O'Donnell, Colleen; Tan, Elizabeth; Xu, Peng
Source:
Biochemical engineering journal 2019 v.144 pp. 209-216
ISSN:
1369-703X
Subject:
malonyl coenzyme A,   etc   ; biosensors; carrying capacity; cell growth; engineering; equations; gene expression; gene expression regulation; genes; hysteresis; kinetics; protein degradation; simulation models; students; transcription (genetics); transcription factors;     Show all 16 Subjects
Abstract:
... Transcriptional factor-based biosensor has been widely used to reprogram and adjust cellular activity to the changing environment. These biosensors translate an internal cellular signal to a transcriptional reporter output. Previous examples have demonstrated transcriptional factor (TF) cross-talk could lead to complex gene expression dynamics. In this report, we formulated a mechanism-based kinet ...
DOI:
10.1016/j.bej.2019.02.005

8. Acyl-coenzyme A:(holo-acyl carrier protein) transacylase enzymes as templates for engineering

Author:
Marcella, Aaron M.; Barb, Adam W.
Source:
Applied microbiology and biotechnology 2018 v.102 no.15 pp. 6333-6341
ISSN:
0175-7598
Subject:
malonyl coenzyme A,   etc   ; Escherichia coli; biosynthesis; engineering; enzymes; enzymology; fatty acids; models; mutation; polyketides;     Show all 10 Subjects
Abstract:
... This review will cover the structure, enzymology, and related aspects that are important for structure-based engineering of the transacylase enzymes from fatty acid biosynthesis and polyketide synthesis. Furthermore, this review will focus on in vitro characteristics and not cover engineering of the upstream or downstream reactions or strategies to manipulate metabolic flux in vivo. The malonyl-co ...
DOI:
10.1007/s00253-018-9114-2

9. Synthetic Biology Expands the Industrial Potential of Yarrowia lipolytica

Author:
Markham, Kelly A.; Alper, Hal S.
Source:
Trends in biotechnology 2018 v.36 no.10 pp. 1085-1095
ISSN:
0167-7799
Subject:
malonyl coenzyme A,   etc   ; Yarrowia lipolytica; acetyl coenzyme A; gene editing; metabolic engineering; synthetic biology; tricarboxylic acid cycle; yeasts;     Show all 8 Subjects
Abstract:
... The oleaginous yeast Yarrowia lipolytica is quickly emerging as the most popular non-conventional (i.e., non-model organism) yeast in the bioproduction field. With a high propensity for flux through tricarboxylic acid (TCA) cycle intermediates and biological precursors such as acetyl-CoA and malonyl-CoA, this host is especially well suited to meet our industrial chemical production needs. Recent p ...
DOI:
10.1016/j.tibtech.2018.05.004

10. Biosynthesis of nervonic acid and perspectives for its production by microalgae and other microorganisms

Author:
Fan, Yong; Meng, Hui-Min; Hu, Guang-Rong; Li, Fu-Li
Source:
Applied microbiology and biotechnology 2018 v.102 no.7 pp. 3027-3035
ISSN:
0175-7598
Subject:
malonyl coenzyme A,   etc   ; biosynthesis; genes; heterologous gene expression; mammals; microalgae; nervonic acid; neurodevelopment; oleic acid; plants (botany); yeasts;     Show all 11 Subjects
Abstract:
... Nervonic acid (NA) is a major very long-chain monounsaturated fatty acid found in the white matter of mammalian brains, which plays a critical role in the treatment of psychotic disorders and neurological development. In the nature, NA has been synthesized by a handful plants, fungi, and microalgae. Although the metabolism of fatty acid has been studied for decades, the biosynthesis of NA has yet ...
DOI:
10.1007/s00253-018-8859-y

11. Structural and biochemical characterization of the plant type III polyketide synthases of the liverwort Marchantia paleacea

Author:
Yu, Hai-Na; Liu, Xin-Yan; Gao, Shuai; Sun, Bin; Zheng, Hong-Bo; Ji, Mei; Cheng, Ai-Xia; Lou, Hong-Xiang
Source:
Plant physiology and biochemistry 2018 v.125 pp. 95-105
ISSN:
0981-9428
Subject:
malonyl coenzyme A,   etc   ; Marchantia; chalcone; condensation reactions; crystal structure; functional diversity; mosses and liverworts; mutants; polyketide synthases; prototypes; trihydroxystilbene synthase;     Show all 11 Subjects
Abstract:
... Chalcone synthases (CHSs) of the type III polyketide synthases (PKSs), catalyze the formation of a tetraketide intermediate from a CoA-tethered starter and malonyl-CoA but use different cyclization mechanisms to produce distinct chemical scaffolds. Herein, we characterized CHS and CHS-like enzymes (designated MpCHS and MpCHSL1, 2 and 3) from Marchantia paleacea and determined the crystal structure ...
DOI:
10.1016/j.plaphy.2018.01.030

12. The emerging role of the mitochondrial fatty-acid synthase (mtFASII) in the regulation of energy metabolism

Author:
Wehbe, Zeinab; Behringer, Sidney; Alatibi, Khaled; Watkins, David; Rosenblatt, David; Spiekerkoetter, Ute; Tucci, Sara
Source:
Biochimica et biophysica acta 2019 v.1864 no.11 pp. 1629-1643
ISSN:
1388-1981
Subject:
malonyl coenzyme A,   etc   ; amino acids; beta oxidation; biosynthesis; cardiolipins; catalytic activity; children; coma; energy; energy metabolism; fatty acids; fatty-acid synthase; fibroblasts; glycolysis; homeostasis; hypoglycemia; inherited metabolic diseases; ketosis; mitochondria; mutation; palmitoylation; patients; phenotype; proteome; proteomics; sphingomyelins;     Show all 26 Subjects
Abstract:
... Malonyl-CoA synthetase (ACSF3) catalyzes the first step of the mitochondrial fatty acid biosynthesis (mtFASII). Mutations in ACSF3 cause CMAMMA a rare inborn error of metabolism. The clinical phenotype is very heterogeneous, with some patients presenting with neurologic manifestations. In some children, presenting symptoms such as coma, ketoacidosis and hypoglycemia are suggestive of an intermedia ...
DOI:
10.1016/j.bbalip.2019.07.012

13. Improved lovastatin production by inhibiting (+)-geodin biosynthesis in Aspergillus terreus

Author:
Hasan, Hanan; Abd Rahim, Muhamad Hafiz; Campbell, Leona; Carter, Dee; Abbas, Ali; Montoya, Alejandro
Source:
New biotechnology 2019 v.52 pp. 19-24
ISSN:
1871-6784
Subject:
malonyl coenzyme A,   etc   ; Aspergillus terreus; acetyl coenzyme A; biosynthesis; byproducts; carbon; cholesterol; emodin; enzyme activity; feedstocks; gene targeting; genes; glycerol; hygromycin B; lactose; lovastatin; mutants; polyketide synthases; secondary metabolites;     Show all 19 Subjects
Abstract:
... Lovastatin is widely prescribed to reduce elevated levels of cholesterol and prevent heart-related diseases. Cultivation of Aspergillus terreus (ATCC 20542) with carbohydrates or low-value feedstocks such as glycerol produces lovastatin as a secondary metabolite and (+)-geodin as a by-product. An A. terreus mutant strain was developed (gedCΔ) with a disrupted (+)-geodin biosynthesis pathway. The g ...
DOI:
10.1016/j.nbt.2019.04.003

14. Evolvement of nutraceutical onion plants engineered for resveratrol biosynthetic pathway

Author:
Naini, Raju; Pavankumar, P.; Prabhakar, S.; Kancha, Rama Krishna; Rao, Khareedu Venkateswara; Reddy, Vudem Dashavantha
Source:
Plant cell reports 2019 v.38 no.9 pp. 1127-1137
ISSN:
0721-7714
Subject:
malonyl coenzyme A,   etc   ; Agrobacterium radiobacter; Southern blotting; Vitis; biochemical pathways; biosynthesis; bulbs; callus; dietary supplements; engineering; genes; genetic engineering; glycosylation; grapes; haploidy; leaves; liquid chromatography; longevity; onions; polymerase chain reaction; resveratrol; transgenic plants; trihydroxystilbene synthase; yeasts;     Show all 24 Subjects
Abstract:
... KEY MESSAGE: Genetically engineered onion expressing codon-optimized VvSTS1 gene accumulated stilbenes and extended life span in yeast and can serve as potential nutraceutical. Resveratrol (RV) is a natural polyphenolic compound found in certain plant species including grapes. RV is well known for its nutraceutical properties and to assuage several disease conditions. Onion is the second most cons ...
DOI:
10.1007/s00299-019-02432-y

15. Understanding lipogenesis by dynamically profiling transcriptional activity of lipogenic promoters in Yarrowia lipolytica

Author:
Liu, Huan; Marsafari, Monireh; Deng, Li; Xu, Peng
Source:
Applied microbiology and biotechnology 2019 v.103 no.7 pp. 3167-3179
ISSN:
0175-7598
Subject:
malonyl coenzyme A,   etc   ; NADP (coenzyme); Yarrowia lipolytica; acetates; acetyl coenzyme A; amino acids; beta oxidation; carbon; fatty acids; fuels; glycolysis; kinetics; lipogenesis; mannitol; nitrogen; oxidation; pentose phosphate cycle; pyruvic acid; starvation; transcription (genetics); tricarboxylic acid cycle;     Show all 21 Subjects
Abstract:
... Lipogenesis is a complicated process involving global transcriptional reprogramming of lipogenic pathways. It is commonly believed that nitrogen starvation triggers a metabolic shift that reroutes carbon flux from Krebs cycles to lipogenesis. In this study, we systematically surveyed and dynamically profiled the transcriptional activity of 22 lipogenic promoters aiming to delineate a picture how n ...
DOI:
10.1007/s00253-019-09664-8

16. Increased sesqui- and triterpene production by co-expression of HMG-CoA reductase and biotin carboxyl carrier protein in tobacco (Nicotiana benthamiana)

Author:
Lee, Ah-Reum; Kwon, Moonhyuk; Kang, Min-Kyoung; Kim, Jeonghan; Kim, Soo-Un; Ro, Dae-Kyun
Source:
Metabolic engineering 2019 v.52 pp. 20-28
ISSN:
1096-7176
Subject:
malonyl coenzyme A,   etc   ; Nicotiana benthamiana; acetyl coenzyme A; acetyl-CoA carboxylase; additive effect; alpha-bisabolol; biochemical pathways; biosynthesis; biotin; cytosol; diterpenoids; fatty acids; gene expression; hydroxymethylglutaryl-CoA reductases; industrial applications; isomers; leaves; monoterpenoids; phosphates; tobacco; triterpenoids;     Show all 21 Subjects
Abstract:
... Terpenoids are the most diverse natural products with many industrial applications and are all synthesized from simple precursors, isopentenyl diphosphate (IPP) and its isomer dimethylallyl diphosphate (DMAPP). In plants, IPP is synthesized by two distinct metabolic pathways – cytosolic mevalonate (MVA) pathway for C15 sesquiterpene and C30 triterpene, and plastidic methylerythritol phosphate (MEP ...
DOI:
10.1016/j.ymben.2018.10.008

17. Molecular cloning and functional analysis of a biphenyl phytoalexin-specific O-methyltransferase from apple cell suspension cultures

Author:
Sarkate, Amol; Saini, Shashank Sagar; Gaid, Mariam; Teotia, Deepa; Mir, Javid Iqbal; Agrawal, Pawan Kumar; Beerhues, Ludger; Sircar, Debabrata
Source:
Planta 2019 v.249 no.3 pp. 677-691
ISSN:
0032-0935
Subject:
malonyl coenzyme A,   etc   ; Malus domestica; Nicotiana benthamiana; Venturia inaequalis; apples; biosynthesis; biphenyl; cell suspension culture; complementary DNA; cultivars; dibenzofuran; elicitors; genes; leaves; methyltransferases; molecular cloning; phytoalexins; polyketides; regioselectivity; substrate specificity; yellow fluorescent protein;     Show all 21 Subjects
Abstract:
... MAIN CONCLUSION: This manuscript describes the cloning and functional characterization of a biphenyl phytoalexin biosynthetic gene, 3,5-dihydroxybiphenyl O-methyltransferase from elicitor-treated cell cultures of scab resistant apple cultivar ‘Florina’. Apples belong to the subtribe Malinae of the Rosaceae family. Biphenyls and dibenzofurans are the specialized phytoalexins of Malinae, of which au ...
DOI:
10.1007/s00425-018-3031-6

18. Production of plant-specific flavones baicalein and scutellarein in an engineered E. coli from available phenylalanine and tyrosine

Author:
Li, Jianhua; Tian, Chenfei; Xia, Yuhui; Mutanda, Ishmael; Wang, Kaibo; Wang, Yong
Source:
Metabolic engineering 2019 v.52 pp. 124-133
ISSN:
1096-7176
Subject:
malonyl coenzyme A,   etc   ; Arabidopsis thaliana; Escherichia coli; Medicago sativa; Oriental traditional medicine; Petroselinum crispum; Petunia hybrida; Rhodotorula; Scutellaria baicalensis; antineoplastic activity; apigenin; biochemical pathways; chalcone isomerase; chrysin; fermentation; genes; hosts; medicinal plants; naringenin-chalcone synthase; phenylalanine; phenylalanine ammonia-lyase; tyrosine;     Show all 22 Subjects
Abstract:
... Baicalein and scutellarein are bioactive flavones found in the medicinal plant Scutellaria baicalensis Georgi, used in traditional Chinese medicine. Extensive previous work has demonstrated the broad biological activity of these flavonoids, such as antifibrotic, antiviral and anticancer properties. However, their supply from plant material is insufficient to meet demand. Here, to provide an altern ...
DOI:
10.1016/j.ymben.2018.11.008

19. Effects of acetoacetyl-CoA synthase expression on production of farnesene in Saccharomyces cerevisiae

Author:
Tippmann, Stefan; Ferreira, Raphael; Siewers, Verena; Nielsen, Jens; Chen, Yun
Source:
Journal of industrial microbiology & biotechnology 2017 v.44 no.6 pp. 911-922
ISSN:
1367-5435
Subject:
malonyl coenzyme A,   etc   ; Saccharomyces cerevisiae; Streptomyces glaucescens; acetyl coenzyme A; acetyl-CoA acetyltransferase; farnesene; phenotype; thermodynamics;     Show all 8 Subjects
Abstract:
... Efficient production of sesquiterpenes in Saccharomyces cerevisiae requires a high flux through the mevalonate pathway. To achieve this, the supply of acetyl-CoA plays a crucial role, partially because nine moles of acetyl-CoA are necessary to produce one mole of farnesyl diphosphate, but also to overcome the thermodynamic constraint imposed on the first reaction, in which acetoacetyl-CoA is produ ...
DOI:
10.1007/s10295-017-1911-6
PubMed:
28185100
PubMed Central:
PMC5432608

20. Biotin-independent strains of Escherichia coli for enhanced streptavidin production

Author:
Jeschek, Markus; Bahls, Maximilian O.; Schneider, Veronika; Marlière, Philippe; Ward, Thomas R.; Panke, Sven
Source:
Metabolic engineering 2017 v.40 pp. 33-40
ISSN:
1096-7176
Subject:
malonyl coenzyme A,   etc   ; Corynebacterium glutamicum; Escherichia coli; biosynthesis; biotin; carboxylation; fatty acids; streptavidin; toxicity;     Show all 9 Subjects
Abstract:
... Biotin is an archetypal vitamin used as cofactor for carboxylation reactions found in all forms of life. However, biotin biosynthesis is an elaborate multi-enzymatic process and metabolically costly. Moreover, many industrially relevant organisms are incapable of biotin synthesis resulting in the requirement to supplement defined media. Here we describe the creation of biotin-independent strains o ...
DOI:
10.1016/j.ymben.2016.12.013