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maltose, etc ; Arabidopsis; beta-amylase; genes; leaf extracts; learning; proteins; starch; Show all 8 Subjects
Abstract:
... Multi-gene families present a rich research area to study how related proteins evolve to acquire new structures and functions. The β-amylase (BAM) gene family is named for catalytic members’ ability to hydrolyze starch into maltose units. However, the family also contains proteins that are catalytically inactive, have additional domains, or are not localized with a starch substrate. Here we review ...
... Staphylococcus aureus (S. aureus) is a leading cause of keratitis worldwide and a significant threat to healthy vision. Pathological manifestations of bacterial keratitis (BK) caused by S. aureus involve stromal opacity, edema and neovascularization of an inflamed cornea, requiring immediate medical attention. Thus, S. aureus-induced keratitis is a devastating ocular infection that can lead to bli ...
... Fully biodegradable protein–polymer conjugates, namely, MBP-PMeEP (maltose binding protein–poly methyl-ethylene phosphonate), have been investigated in order to understand the role of polymer solvation on protein flexibility. Using elastic and quasi-elastic incoherent neutron scattering, in combination with partially deuterated conjugate systems, we are able to disentangle the polymer dynamics fro ...
... Determination of the oligosaccharide composition in different wort samples is important to monitor their change during the brewing process with different yeast types. In our work, the concentration of fermentable and non-fermentable sugars were monitored by capillary electrophoresis to observe the effect of two different types of yeasts, Saccharomyces pastorianus and Saccharomycodes ludwigii. The ...
... Emulsifiers derived from renewable resources such as sucrose and fatty acids are high volume commodity chemicals and currently produced by traditional chemical synthesis techniques that lack the capacity to form the most desirable monoesters (of sucrose) in a selective and efficient fashion. The development of new emulsifiers (surfactants) from alternate, structurally simpler but nevertheless abun ...
... As a demonstration that second-order nonlinear optical microscopy is a powerful tool for rice grain science, we observed second-harmonic generation (SHG) images of amylose-free glutinous rice and amylose-containing nonglutinous rice grains. The images obtained from SHG microscopy and photographs of the iodine-stained starch granules indicate that the distribution of starch types in the embryo-faci ...
... The aim was to determine the mycotoxin transfer rate into beer during a semi-industrial production process and the effect of fungicide treatment in the field on mycotoxins concentrations in beer. To ensure the usual practical agronomical conditions, sample A was treated with fungicide Prosaro® 250, and sample B was infected with Fusarium culmorum spores, in order to obtain infected malt. Malt was ...
maltose, etc ; beta-casein; beta-lactoglobulin; glycation; lactalbumin; lactose; lysine; monosaccharides; trisaccharides; whey protein; Show all 10 Subjects
Abstract:
... This paper set out to differentiate the Maillard induced glycation reactivity of individual milk proteins using different saccharides under well-defined reaction conditions. α-Lactalbumin, β-lactoglobulin and β-casein were incubated with mono-, di- and trisaccharides in the dry state under standardised buffered conditions and glycation was expressed relative to the available reactive groups per pr ...
... Herein, we report a fairly simple and environmentally friendly approach for the fabrication of starch-based magnetic polymer beads (SMPBs) with uniform shape and size through spontaneous rearrangement of short-chain glucan (SCG) produced by enzymatic debranching of waxy maize starch. The paramagnetic materials, dextran-coated iron oxide nanoparticles (Dex@IONPs), were readily incorporated into the ...
... The process for production of trehalose using trehalose synthase (TreS) to convert maltose into trehalose in one step is highly desirable in the industry. Nonetheless, the studies on industrial-scale production of trehalose by recombinant TreS in Escherichia coli are still scarce. In this study, a TreS from Pseudomonas putida ATCC47054 was expressed in E. coli BL21(DE3) via plasmids pET15b and pET ...
maltose, etc ; acetylene; chemical reactions; chemical structure; encapsulation; hydrogen bonding; lipophilicity; monosaccharides; organic chemistry; phenol; pyridines; Show all 11 Subjects
Abstract:
... A nonplanar macrocycle consisting of four pyridine–acetylene–phenol units was developed as a host for saccharide guest molecules. The macrocycle was found to strongly associate with a lipophilic maltose derivative, with an association constant of 10⁷ M–¹, over monosaccharide derivatives, for which much smaller association constants were determined, ranging from 10³ M–¹ to 10⁴ M–¹. The macrocycle w ...
maltose, etc ; chemical structure; electrical conductivity; fructose; glucose; honey; magnetic fields; pH; sucrose; temperature; water activity; Poland; Show all 12 Subjects
Abstract:
... The content of glucose, fructose, sucrose, maltose and water were determined for multiflorous honey of Great Poland. The measurements were carried out for different fractions of honey and also for the liquefied honey at 40 °C. Water activity and pH were both determined for all samples. A new method of recognizing liquefied honey is proposed based on the water influence on pH and the monosaccharide ...
maltose, etc ; ABC transporters; Escherichia coli; adenosinetriphosphatase; electron paramagnetic resonance spectroscopy; enzyme activity; models; mutants; mutation; Show all 9 Subjects
Abstract:
... MalG511 is a genetically selected binding-protein-independent mutant of the Escherichia coli maltose transporter MalFGK₂, which retains specificity for maltose and shows a high basal ATPase activity in the absence of maltose binding protein (MBP). It shows an intriguing biphasic behavior in maltose transport assays in the presence of MBP, with low levels of MBP stimulating the activity and higher ...
maltose, etc ; cosmetics; drugs; foods; glucose; invertebrates; physicochemical properties; prices; starch; trehalose; Show all 10 Subjects
Abstract:
... Trehalose (α-D-glucopyranosyl-(1 → 1)-α-D-glucopyranoside) is a non-reducing disaccharide composed of two glucose molecules linked by an α,α-1,1-glycosidic bond. It possesses physicochemical properties, which account for its biological roles in a variety of prokaryotic and eukaryotic organisms and invertebrates. Intensive studies of trehalose gradually uncovered its functions, and its applications ...
maltose, etc ; Bacillus cereus; carbon; fructose; genes; glucose; microbial growth; microorganisms; phosphatidylcholines; phospholipase D; Show all 10 Subjects
Abstract:
... In the present study, a new strain, Bacillus cereus ZY12, producing phospholipase D (PLD) was identified. The expression of PLD in this strain was found to be induced by its substrate, phosphatidylcholine (PC), and completely silenced by other carbon sources, such as glucose, fructose, and maltose, which are generally used in microbial growth cultures, thus presenting a unique expression pattern d ...
maltose, etc ; alpha-amylase; asparagine; chitosan; enzyme activity; lysine; pH; starch; syrups; temperature; Show all 10 Subjects
Abstract:
... Maltose syrups have got wide-range utilizations in a variety of applications from bakery to drug-development. α-Amylases are among the most widely utilized industrial enzymes due to their high specificity in production of maltose syrup from starch. However, enzymes are not stable in ex vivo conditions towards alteration in pH, temperature, and such other parameters as high salt concentrations and ...
maltose, etc ; Bacillus pumilus; Escherichia coli; antioxidants; ferulic acid; glycosylation; pH; regioselectivity; transferases; Show all 9 Subjects
Abstract:
... Ferulic acid is a naturally occurring antioxidant, and its glycosylation is a potential approach used to improve its pharmaceutical properties. The regioselective glycosylation of ferulic acid was successfully achieved by pH adjustment using glycosyltransferase GTBP1 from Bacillus pumilus BF1. 4-O-β-d-Glucopyranosylferulic acid was efficiently synthesized with molar yield of 90.2% by the recombina ...
maltose, etc ; DNA; RNA; apoptosis; cell lines; cytotoxicity; dendrimers; drug resistance; drugs; nanocarriers; nucleosides; pharmacodynamics; pharmacokinetics; Show all 13 Subjects
Abstract:
... Fludarabine, a nucleoside analogue antimetabolite, has complicated pharmacokinetics requiring facilitated transmembrane transport and intracellular conversion to triphosphate nucleotide form (Ara-FATP), causing it to be susceptible to emergence of drug resistance. We are testing a promising strategy to improve its clinical efficacy by direct delivery of Ara-FATP utilizing a biocompatible glycodend ...
maltose, etc ; Medicago; glycomics; ions; isomers; leaves; mass spectrometry; quantitative analysis; sucrose; Show all 9 Subjects
Abstract:
... Saccharide isomer differentiation has been a challenge in glycomics, as the lack of technology to decipher fully the diverse structures of compositions, linkages, and anomeric configurations. Several mass spectrometry-based methods have been applied to the discrimination of disaccharide isomers, but limited quantitative analyses have been reported. In the present study, MALDI-LIFT-TOF/TOF has been ...
maltose, etc ; binding capacity; cytochrome c; electrospray ionization mass spectrometry; hydrophobicity; ligands; maltotriose; Show all 7 Subjects
Abstract:
... Noncovalent interactions between ligands and targeting proteins are essential for understanding molecular mechanisms of proteins. In this work, we investigated the interaction of Cytochrome c (Cyt c) with maltoligosaccharides, namely maltose (Mal II), maltotriose (Mal III), maltotetraose (Mal IV), maltopentaose (Mal V), maltohexaose (Mal VI) and maltoheptaose (Mal VII). Using electrospray ionizati ...