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1. Inhalable mRNA vaccines for respiratory diseases: a roadmap

Author:
Esther H Roh; Catherine A Fromen; Millicent O Sullivan
Source:
Current opinion in biotechnology 2022 v.74 pp. 104-109
ISSN:
0958-1669
Subject:
messenger RNA,   etc   ; biotechnology; memory; respiratory tract diseases; therapeutics;     Show all 5 Subjects
Abstract:
... Global implementation of messenger RNA (mRNA) vaccines represents an enormous advance with far-reaching implications for respiratory disease treatment. mRNA vaccines offer exceptional efficacy and versatile capacity to be adapted to new viruses and variants; however, critical questions remain regarding immune persistence and formulation stability. This represents a significant opportunity for deve ...
DOI:
10.1016/j.copbio.2021.10.017

2. Comprehensive analysis of the LncRNAs, MiRNAs, and MRNAs acting within the competing endogenous RNA network of LGG

Author:
Yiming Ding; Hanjie Liu; Chuanbao Zhang; Zhaoshi Bao; Shuqing Yu
Source:
Genetica 2022 v.150 no.1 pp. 41-50
ISSN:
0016-6707
Subject:
messenger RNA,   etc   ; gene ontology; glioma; microRNA; non-coding RNA; pathogenesis;     Show all 6 Subjects
Abstract:
... Messenger RNA (mRNA) and long noncoding RNA (lncRNA) targets interact via competitive microRNA (miRNA) binding. However, the roles of cancer-specific lncRNAs in the competing endogenous RNA (ceRNA) networks of low-grade glioma (LGG) remain unclear. This study obtained RNA sequencing data for normal solid tissue and LGG primary tumour tissue from The Cancer Genome Atlas database. We used a computat ...
DOI:
10.1007/s10709-021-00145-3

3. How long does the mRNA remains stable in untreated whole bovine blood?

Author:
Rodrigo Giglioti; Bianca Tainá Azevedo; Henrique Nunes de Oliveira; Luciana Morita Katiki; Anibal Eugênio Vercesi Filho; Márcia Cristina de Sena Oliveira; Cintia Hiromi Okino
Source:
Molecular biology reports 2022 v.49 no.1 pp. 789-795
ISSN:
0301-4851
Subject:
messenger RNA,   etc   ; Holstein; blood; gene expression; molecular biology; transcription (genetics);     Show all 6 Subjects
Abstract:
... BACKGROUND: High quality and quantity of messenger RNA (mRNA) are required for accuracy of gene expression studies and other RNA-based downstream applications. Since RNA is considered a labile macromolecular prone to degradation, which may result in falsely altered gene expression patterns, several commercial stabilizing reagents have been developed aiming to keep RNA stable for long period. Howev ...
DOI:
10.1007/s11033-021-06808-w

4. Splicing Variants, Protein-Protein Interactions, and Drug Targeting in Hutchinson-Gilford Progeria Syndrome and Small Cell Lung Cancer

Author:
Bae-Hoon Kim; Tae-Gyun Woo; So-Mi Kang; Soyoung Park; Bum-Joon Park
Source:
Genes 2022 v.13 no.2 pp. -
ISSN:
2073-4425
Subject:
messenger RNA,   etc   ; DNA; drugs; genes; homeostasis; lung neoplasms; proteomics; therapeutics;     Show all 8 Subjects
Abstract:
... Alternative splicing (AS) is a biological operation that enables a messenger RNA to encode protein variants (isoforms) that give one gene several functions or properties. This process provides one of the major sources of use for understanding the proteomic diversity of multicellular organisms. In combination with post-translational modifications, it contributes to generating a variety of protein–p ...
DOI:
10.3390/genes13020165

5. Identification of mRNA 5′ cap-associated proteins in the human malaria parasite Plasmodium falciparum

Author:
Chayaphat Wongsombat; Yodying Yingchutrakul; Nattida Suwanakitti; Kantinan Leetanasaksakul; Sittiruk Roytrakul; Philip James Shaw; Aiyada Aroonsri
Source:
Molecular and biochemical parasitology 2022 v.247 pp. 111443
ISSN:
0166-6851
Subject:
messenger RNA,   etc   ; Plasmodium falciparum; humans; malaria; parasites; parasitology; phosphopyruvate hydratase; proteomics;     Show all 8 Subjects
Abstract:
... Eukaryotic messenger RNA is translated via a 5′ cap-dependent initiation mechanism. Experimental evidence for proteins involved with translation initiation among eukaryotic parasites is lacking, including Plasmodium falciparum, the human malaria parasite. Native P. falciparum proteins from asexual stage parasites were enriched using a 5′ cap affinity matrix. Proteomic analysis of enriched protein ...
DOI:
10.1016/j.molbiopara.2021.111443

6. Analysis Polyadenylation Signal Usage in Sus scrofa

Author:
Yuting Zhang; Jingwen Song; Min Zhang; Zhongyuan Deng
Source:
Animals 2022 v.12 no.2 pp. -
ISSN:
2076-2615
Subject:
messenger RNA,   etc   ; Sus scrofa; gene expression; humans; livestock; mice; sequence analysis; transcription (genetics);     Show all 8 Subjects
Abstract:
... RNA polyadenylation is an important step in the messenger RNA (mRNA) maturation process, and the first step is recognizing the polyadenylation signal (PAS). The PAS type and distribution is a key determinant of post-transcriptional mRNA modification and gene expression. However, little is known about PAS usage and alternative polyadenylation (APA) regulation in livestock species. Recently, sequenc ...
DOI:
10.3390/ani12020194

7. Size and Charge Characterization of Lipid Nanoparticles for mRNA Vaccines

Author:
Camille Malburet; Laurent Leclercq; Jean-François Cotte; Jérôme Thiebaud; Emilie Bazin; Marie Garinot; Hervé Cottet
Source:
Analytical chemistry 2022 v.94 no.11 pp. 4677-4685
ISSN:
1520-6882
Subject:
messenger RNA,   etc   ; analytical chemistry; capillary electrophoresis; cytoplasm; electrostatic interactions; immune response; lipids; vaccines;     Show all 8 Subjects
Abstract:
... Messenger RNA vaccines have come into the spotlight as a promising and adaptive alternative to conventional vaccine approaches. The efficacy of mRNA vaccines relies on the ability of mRNA to reach the cytoplasm of cells, where it can be translated into proteins of interest, allowing it to trigger the immune response. However, unprotected mRNA is unstable and susceptible to degradation by exo- and ...
DOI:
10.1021/acs.analchem.1c04778

8. Functional interplay within the epitranscriptome: Reality or fiction?

Author:
Lina Worpenberg; Chiara Paolantoni; Jean‐Yves Roignant
Source:
BioEssays 2022 v.44 no.2 pp. e2100174
ISSN:
0265-9247
Subject:
messenger RNA,   etc   ; 5-methylcytosine; adenosine; gene expression; inosine; metabolism; non-coding RNA; ribose; transfer RNA;     Show all 9 Subjects
Abstract:
... RNA modifications have recently emerged as an important regulatory layer of gene expression. The most prevalent and reversible modification on messenger RNA (mRNA), N6‐methyladenosine, regulates most steps of RNA metabolism and its dysregulation has been associated with numerous diseases. Other modifications such as 5‐methylcytosine and N1‐methyladenosine have also been detected on mRNA but their ...
DOI:
10.1002/bies.202100174

9. MicroRNA ssa-mir-196a-4 deceases lgr8 expression in testis development of Chinese tongue sole (Cynoglossus semilaevis)

Author:
Lili Tang; Wuxin You; Qian Wang; Fei Huang; Changwei Shao
Source:
Comparative biochemistry and physiology 2022 v.258 pp. 110695
ISSN:
1096-4959
Subject:
messenger RNA,   etc   ; Cynoglossus semilaevis; bioinformatics; cell lines; computer software; cytoplasm; fluorescence; genes; hybridization; microRNA; sex determination;     Show all 11 Subjects
Abstract:
... MicroRNAs (miRNAs) contribute to gonadal development in animals. However, there is little information about miRNA regulation function involved in gonadal development in fish. Our group previously identified sex-related miRNAs of Chinese tongue sole (Cynoglossus semilaevis) during sex determination and differentiation by small RNA sequencing. In the present study, we characterized ssa-mir-196a-4 an ...
DOI:
10.1016/j.cbpb.2021.110695

10. Rapid diagnostic detection of tomato apical stunt viroid based on isothermal reverse transcription-recombinase polymerase amplification

Author:
Natalia Kovalskaya; Rosemarie W. Hammond
Source:
Journal of virological methods 2022 v.300 pp. 114353
ISSN:
0166-0934
Subject:
messenger RNA,   etc   ; Tomato apical stunt viroid; cross reaction; microbial detection; plant pathogens; recombinase polymerase amplification; tomatoes;     Show all 7 Subjects
Abstract:
... Tomato apical stunt viroid (TASVd) is a serious threat to tomato plants that can cause a considerable yield loss. In the present study, two isothermal molecular diagnostic assays based on reverse transcription-recombinase polymerase amplification (RT-RPA) utilizing the AmplifyRP® platform for plant pathogen detection were developed. The results of this research demonstrated distinct specificity of ...
Handle:
10113/7564532
DOI:
10.1016/j.jviromet.2021.114353
CHORUS:
10.1016/j.jviromet.2021.114353

11. Epigenetic regulator BRD4 is involved in cadmium-induced acute kidney injury via contributing to lysosomal dysfunction, autophagy blockade and oxidative stress

Author:
Zhong-Gui Gong; Yuan Zhao; Zhen-Yong Wang; Rui-Feng Fan; Zong-Ping Liu; Lin Wang
Source:
Journal of hazardous materials 2022 v.423 pp. 127110
ISSN:
0304-3894
Subject:
messenger RNA,   etc   ; acetylation; acute kidney injury; autophagy; cadmium; cytotoxicity; epigenetics; genes; heavy metals; nephrotoxicity; oxidative stress; repressor proteins; therapeutics;     Show all 13 Subjects
Abstract:
... Cadmium (Cd) is a known nephrotoxic heavy metal and proximal tubules are the major target of Cd-induced acute kidney injury (AKI). We previously demonstrated that lysosomal dysfunction and dysregulated autophagy contribute to Cd-induced AKI. Recent studies have revealed that bromodomain-containing protein 4 (BRD4) is a transcriptional repressor of autophagy and lysosomal function. Hence, in vivo a ...
DOI:
10.1016/j.jhazmat.2021.127110

12. Variability and evolution of NBS-LRR genes in Agave tequilana and their differential response to Lasiodiplodia infection

Author:
G. Campos-Rivero; J. A. Narváez-Zapata; F. Escalante-Erosa; L. F. Sánchez-Teyer
Source:
European journal of plant pathology 2022 v.162 no.2 pp. 305-327
ISSN:
0929-1873
Subject:
messenger RNA,   etc   ; Agave tequilana; Lasiodiplodia; gene ontology; genes; phylogeny; physiological response; plant pathology; salicylic acid; selection pressure; transcriptome; Mexico;     Show all 12 Subjects
Abstract:
... NBS domain-containing sequences from the Agave tequilana transcriptome shotgun assembly were identified, characterized, and classified based on their physicochemical properties and motif structure, which resulted in a differential response to Lasiodiplodia sp. infection. Agave tequilana is an important crop in Mexico that is susceptible to many pathogens and adverse conditions. In plants, NBS-LRR ...
DOI:
10.1007/s10658-021-02404-7

13. N6-methyladenosine modification underlies messenger RNA metabolism and plant development

Author:
Yanlin Shao; Chui Eng Wong; Lisha Shen; Hao Yu
Source:
Current opinion in plant biology 2021 v.63 pp. 102047
ISSN:
1369-5266
Subject:
messenger RNA,   etc   ; genes; metabolism; methyltransferases; plant development;     Show all 5 Subjects
Abstract:
... RNA modifications constitute an essential layer of gene regulation in living organisms. As the most prevalent internal modification on eukaryotic mRNAs, N⁶-methyladenosine (m⁶A) exists in many plant species and requires the evolutionarily conserved methyltransferases, demethylases, and m⁶A binding proteins for writing, erasing, and reading m⁶A, respectively. In plants, m⁶A affects many aspects of ...
DOI:
10.1016/j.pbi.2021.102047

14. Genome‐wide identification and analysis of sulfatase and sulfatase modifying factor genes in Bemisia tabaci (Hemiptera: Aleyrodidae)

Author:
Jun Ren; Zheng‐Ke Peng; Ze‐Zhong Yang; Li‐Xia Tian; Shao‐Nan Liu; Shao‐Li Wang; Qing‐Jun Wu; Wen Xie; You‐Jun Zhang
Source:
Insect science 2021 v.28 no.6 pp. 1541-1552
ISSN:
1672-9609
Subject:
messenger RNA,   etc   ; Bemisia tabaci; arylsulfatase; cabbage; glucosinolates; mortality;     Show all 6 Subjects
Abstract:
... The invasive pest whitefly (Bemisia tabaci) is a complex species, of which Middle East‐Minor Asia 1 (MEAM1) and Mediterranean (MED) are the two most damaging members. Previous research showed that cabbage is frequently infested with MEAM1 but seldomly with MED, and this difference in performance is associated with glucosinolate (GS) content. Some insects can modify GS using glucosinolate sulfatase ...
DOI:
10.1111/1744-7917.12898

15. Messenger RNA delivery to mitoribosomes – hints from a bacterial toxin

Author:
Francesco Bruni; Yasmin Proctor‐Kent; Robert N. Lightowlers; Zofia M. Chrzanowska‐Lightowlers
Source:
FEBS journal 2021 v.288 no.2 pp. 437-451
ISSN:
1742-464X
Subject:
messenger RNA,   etc   ; bacterial toxins; mammals; mitochondria; translation (genetics);     Show all 5 Subjects
Abstract:
... In mammalian mitochondria, messenger RNA is processed and matured from large primary transcripts in structures known as RNA granules. The identity of the factors and process transferring the matured mRNA to the mitoribosome for translation is unclear. Nascent mature transcripts are believed to associate initially with the small mitoribosomal subunit prior to recruitment of the large subunit to for ...
DOI:
10.1111/febs.15342

16. Dual Skin-Whitening and Anti-wrinkle Function of Low-Molecular-Weight Fish Collagen

Author:
Dakyung Kim; Minhee Lee; Joo Hwan Yang; Joon Sung Yang; Ok-Kyung Kim
Source:
Journal of medicinal food 2022 v.25 no.2 pp. 192-204
ISSN:
1557-7600
Subject:
messenger RNA,   etc   ; antioxidants; collagen; cyclic AMP; cytokines; fibroblasts; fish; functional foods; glutathione; melanocytes; melanogenesis; nitric oxide; photoaging; transcription factors; ultraviolet radiation;     Show all 15 Subjects
Abstract:
... In this study, we investigated the protective effects of low-molecular-weight fish collagen from tilapia against melanogenesis in melanocytes, ultraviolet B (UVB)-irradiated Hs27 skin fibroblasts, and hairless mice. We observed collagen production-related pathways in UVB-irradiated Hs27 skin fibroblasts and hairless mice, and the melanogenesis-related pathways in melanocyte and UVB-irradiated hair ...
DOI:
10.1089/jmf.2021.K.0124

17. Delivering the message: How a novel technology enabled the rapid development of effective vaccines

Author:
Lucy Shapiro; Richard Losick
Source:
Cell 2021 v.184 no.21 pp. 5271-5274
ISSN:
0092-8674
Subject:
messenger RNA,   etc   ; COVID-19 infection; biomedical research; technology; therapeutics;     Show all 5 Subjects
Abstract:
... This year’s Lasker∼Debakey Clinical Research Award honors Katalin Karikó and Drew Weissman for the development of a therapeutic technology based on nucleoside-modification of messenger RNA, enabling the rapid development of the highly effective COVID-19 vaccines. ...
DOI:
10.1016/j.cell.2021.08.019

18. Development of a 3’:5’ digital PCR assay to determine horse mRNA integrity

Author:
Charis Du Cheyne; Yao Chen; Jurgen De Craene; Olivier Thas; Ward De Spiegelaere
Source:
Analytical biochemistry 2021 v.626 pp. 114217
ISSN:
0003-2697
Subject:
messenger RNA,   etc   ; gene expression; horses; reverse transcriptase polymerase chain reaction;     Show all 4 Subjects
Abstract:
... Accurate tools to measure RNA integrity are essential to obtain reliable gene expression data. The reverse transcription quantitative PCR (RT-qPCR) based 3’:5′ assay permits a direct determination of messenger RNA (mRNA) integrity. However, the use of standard curves and the possible effect of PCR inhibitors make this method cumbersome and prone to variation, especially in small samples. Here we d ...
DOI:
10.1016/j.ab.2021.114217

19. Novel rRNA-depletion methods for total RNA sequencing and ribosome profiling developed for avian species

Author:
Hanwen Gu; Yu H. Sun; Xin Zhiguo Li
Source:
Poultry science 2021 v.100 no.9 pp. 101321
ISSN:
0032-5791
Subject:
messenger RNA,   etc   ; cost effectiveness; gene expression; poultry industry; ribosomes; transcriptome;     Show all 6 Subjects
Abstract:
... Deep sequencing of RNAs has greatly aided the study of the transcriptome, enabling comprehensive gene expression profiling and the identification of novel transcripts. While messenger RNAs (mRNAs) are of the greatest interest in gene expression studies as they encode for proteins, mRNAs make up only 3 to 5% of total RNAs, with the majority comprising ribosomal RNAs (rRNAs). Therefore, applications ...
DOI:
10.1016/j.psj.2021.101321
CHORUS:
10.1016/j.psj.2021.101321

20. A Self-Made Optical Tweezers Integrated Upconversion Luminescence Confocal Scanning Instrument Enables Stable and Noninvasive Long-Term In Situ Imaging a Single Suspension Cell Under Exceptionally Efficient Luminescent Resonance Energy Transfer Sensing

Author:
Cheng-Yu Li; Jun-Xian Liu; Meng-Kun Xin; Jing-Wei He; Ya-Ling Chen
Source:
Analytical chemistry 2022 v.94 no.18 pp. 6909-6917
ISSN:
1520-6882
Subject:
messenger RNA,   etc   ; DNA; analytical chemistry; calcium; energy transfer; fluorescence; liquids;     Show all 7 Subjects
Abstract:
... It is necessary to explore labeling probes with worthy optical properties and a noninvasive fluorescence imaging manner for stable long-term in situ measuring a single suspension cell. In response to these goals, we herein make a breakthrough on two fronts. On one hand, a co-sensitizer-induced efficient 808 nm near-infrared light-excited luminescence-confined upconversion nanoparticle with a low t ...
DOI:
10.1021/acs.analchem.2c01338