An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
messengerRNA, etc ; biotechnology; memory; respiratory tract diseases; therapeutics; Show all 5 Subjects
Abstract:
... Global implementation of messenger RNA (mRNA) vaccines represents an enormous advance with far-reaching implications for respiratory disease treatment. mRNA vaccines offer exceptional efficacy and versatile capacity to be adapted to new viruses and variants; however, critical questions remain regarding immune persistence and formulation stability. This represents a significant opportunity for deve ...
messengerRNA, etc ; gene ontology; glioma; microRNA; non-coding RNA; pathogenesis; Show all 6 Subjects
Abstract:
... Messenger RNA (mRNA) and long noncoding RNA (lncRNA) targets interact via competitive microRNA (miRNA) binding. However, the roles of cancer-specific lncRNAs in the competing endogenous RNA (ceRNA) networks of low-grade glioma (LGG) remain unclear. This study obtained RNA sequencing data for normal solid tissue and LGG primary tumour tissue from The Cancer Genome Atlas database. We used a computat ...
messengerRNA, etc ; Holstein; blood; gene expression; molecular biology; transcription (genetics); Show all 6 Subjects
Abstract:
... BACKGROUND: High quality and quantity of messenger RNA (mRNA) are required for accuracy of gene expression studies and other RNA-based downstream applications. Since RNA is considered a labile macromolecular prone to degradation, which may result in falsely altered gene expression patterns, several commercial stabilizing reagents have been developed aiming to keep RNA stable for long period. Howev ...
messengerRNA, etc ; DNA; drugs; genes; homeostasis; lung neoplasms; proteomics; therapeutics; Show all 8 Subjects
Abstract:
... Alternative splicing (AS) is a biological operation that enables a messenger RNA to encode protein variants (isoforms) that give one gene several functions or properties. This process provides one of the major sources of use for understanding the proteomic diversity of multicellular organisms. In combination with post-translational modifications, it contributes to generating a variety of protein–p ...
messengerRNA, etc ; Plasmodium falciparum; humans; malaria; parasites; parasitology; phosphopyruvate hydratase; proteomics; Show all 8 Subjects
Abstract:
... Eukaryotic messenger RNA is translated via a 5′ cap-dependent initiation mechanism. Experimental evidence for proteins involved with translation initiation among eukaryotic parasites is lacking, including Plasmodium falciparum, the human malaria parasite. Native P. falciparum proteins from asexual stage parasites were enriched using a 5′ cap affinity matrix. Proteomic analysis of enriched protein ...
messengerRNA, etc ; Sus scrofa; gene expression; humans; livestock; mice; sequence analysis; transcription (genetics); Show all 8 Subjects
Abstract:
... RNA polyadenylation is an important step in the messenger RNA (mRNA) maturation process, and the first step is recognizing the polyadenylation signal (PAS). The PAS type and distribution is a key determinant of post-transcriptional mRNA modification and gene expression. However, little is known about PAS usage and alternative polyadenylation (APA) regulation in livestock species. Recently, sequenc ...
messengerRNA, etc ; analytical chemistry; capillary electrophoresis; cytoplasm; electrostatic interactions; immune response; lipids; vaccines; Show all 8 Subjects
Abstract:
... Messenger RNA vaccines have come into the spotlight as a promising and adaptive alternative to conventional vaccine approaches. The efficacy of mRNA vaccines relies on the ability of mRNA to reach the cytoplasm of cells, where it can be translated into proteins of interest, allowing it to trigger the immune response. However, unprotected mRNA is unstable and susceptible to degradation by exo- and ...
messengerRNA, etc ; 5-methylcytosine; adenosine; gene expression; inosine; metabolism; non-coding RNA; ribose; transfer RNA; Show all 9 Subjects
Abstract:
... RNA modifications have recently emerged as an important regulatory layer of gene expression. The most prevalent and reversible modification on messenger RNA (mRNA), N6‐methyladenosine, regulates most steps of RNA metabolism and its dysregulation has been associated with numerous diseases. Other modifications such as 5‐methylcytosine and N1‐methyladenosine have also been detected on mRNA but their ...
messengerRNA, etc ; Cynoglossus semilaevis; bioinformatics; cell lines; computer software; cytoplasm; fluorescence; genes; hybridization; microRNA; sex determination; Show all 11 Subjects
Abstract:
... MicroRNAs (miRNAs) contribute to gonadal development in animals. However, there is little information about miRNA regulation function involved in gonadal development in fish. Our group previously identified sex-related miRNAs of Chinese tongue sole (Cynoglossus semilaevis) during sex determination and differentiation by small RNA sequencing. In the present study, we characterized ssa-mir-196a-4 an ...
... Tomato apical stunt viroid (TASVd) is a serious threat to tomato plants that can cause a considerable yield loss. In the present study, two isothermal molecular diagnostic assays based on reverse transcription-recombinase polymerase amplification (RT-RPA) utilizing the AmplifyRP® platform for plant pathogen detection were developed. The results of this research demonstrated distinct specificity of ...
messengerRNA, etc ; acetylation; acute kidney injury; autophagy; cadmium; cytotoxicity; epigenetics; genes; heavy metals; nephrotoxicity; oxidative stress; repressor proteins; therapeutics; Show all 13 Subjects
Abstract:
... Cadmium (Cd) is a known nephrotoxic heavy metal and proximal tubules are the major target of Cd-induced acute kidney injury (AKI). We previously demonstrated that lysosomal dysfunction and dysregulated autophagy contribute to Cd-induced AKI. Recent studies have revealed that bromodomain-containing protein 4 (BRD4) is a transcriptional repressor of autophagy and lysosomal function. Hence, in vivo a ...
... NBS domain-containing sequences from the Agave tequilana transcriptome shotgun assembly were identified, characterized, and classified based on their physicochemical properties and motif structure, which resulted in a differential response to Lasiodiplodia sp. infection. Agave tequilana is an important crop in Mexico that is susceptible to many pathogens and adverse conditions. In plants, NBS-LRR ...
messengerRNA, etc ; genes; metabolism; methyltransferases; plant development; Show all 5 Subjects
Abstract:
... RNA modifications constitute an essential layer of gene regulation in living organisms. As the most prevalent internal modification on eukaryotic mRNAs, N⁶-methyladenosine (m⁶A) exists in many plant species and requires the evolutionarily conserved methyltransferases, demethylases, and m⁶A binding proteins for writing, erasing, and reading m⁶A, respectively. In plants, m⁶A affects many aspects of ...
messengerRNA, etc ; Bemisia tabaci; arylsulfatase; cabbage; glucosinolates; mortality; Show all 6 Subjects
Abstract:
... The invasive pest whitefly (Bemisia tabaci) is a complex species, of which Middle East‐Minor Asia 1 (MEAM1) and Mediterranean (MED) are the two most damaging members. Previous research showed that cabbage is frequently infested with MEAM1 but seldomly with MED, and this difference in performance is associated with glucosinolate (GS) content. Some insects can modify GS using glucosinolate sulfatase ...
messengerRNA, etc ; bacterial toxins; mammals; mitochondria; translation (genetics); Show all 5 Subjects
Abstract:
... In mammalian mitochondria, messenger RNA is processed and matured from large primary transcripts in structures known as RNA granules. The identity of the factors and process transferring the matured mRNA to the mitoribosome for translation is unclear. Nascent mature transcripts are believed to associate initially with the small mitoribosomal subunit prior to recruitment of the large subunit to for ...
... In this study, we investigated the protective effects of low-molecular-weight fish collagen from tilapia against melanogenesis in melanocytes, ultraviolet B (UVB)-irradiated Hs27 skin fibroblasts, and hairless mice. We observed collagen production-related pathways in UVB-irradiated Hs27 skin fibroblasts and hairless mice, and the melanogenesis-related pathways in melanocyte and UVB-irradiated hair ...
messengerRNA, etc ; COVID-19 infection; biomedical research; technology; therapeutics; Show all 5 Subjects
Abstract:
... This year’s Lasker∼Debakey Clinical Research Award honors Katalin Karikó and Drew Weissman for the development of a therapeutic technology based on nucleoside-modification of messenger RNA, enabling the rapid development of the highly effective COVID-19 vaccines. ...
messengerRNA, etc ; gene expression; horses; reverse transcriptase polymerase chain reaction; Show all 4 Subjects
Abstract:
... Accurate tools to measure RNA integrity are essential to obtain reliable gene expression data. The reverse transcription quantitative PCR (RT-qPCR) based 3’:5′ assay permits a direct determination of messenger RNA (mRNA) integrity. However, the use of standard curves and the possible effect of PCR inhibitors make this method cumbersome and prone to variation, especially in small samples. Here we d ...
messengerRNA, etc ; cost effectiveness; gene expression; poultry industry; ribosomes; transcriptome; Show all 6 Subjects
Abstract:
... Deep sequencing of RNAs has greatly aided the study of the transcriptome, enabling comprehensive gene expression profiling and the identification of novel transcripts. While messenger RNAs (mRNAs) are of the greatest interest in gene expression studies as they encode for proteins, mRNAs make up only 3 to 5% of total RNAs, with the majority comprising ribosomal RNAs (rRNAs). Therefore, applications ...
messengerRNA, etc ; DNA; analytical chemistry; calcium; energy transfer; fluorescence; liquids; Show all 7 Subjects
Abstract:
... It is necessary to explore labeling probes with worthy optical properties and a noninvasive fluorescence imaging manner for stable long-term in situ measuring a single suspension cell. In response to these goals, we herein make a breakthrough on two fronts. On one hand, a co-sensitizer-induced efficient 808 nm near-infrared light-excited luminescence-confined upconversion nanoparticle with a low t ...