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1. Exploration of Dietetics Graduate Students’ Experience in a Flipped Course Using Learning Reflections

Author:
Vollmer, Rachel L.; Drake, Teresa
Source:
Journal of nutrition education and behavior 2020 v.52 no.4 pp. 407-414
ISSN:
1499-4046
Subject:
metabolism,   etc   ; autumn; dietetics; graduate students; learning; teachers;     Show all 6 Subjects
Abstract:
... Evaluate graduate students’ experience in a flipped metabolism course using periodic learning reflections.Two cohorts of graduate students (n = 17, total) were recruited from an advanced metabolism course using a flipped learning approach during the fall 2016 and 2017 semesters. After course completion, 2 investigators used content analysis to develop themes from 8 structured learning reflections. ...
DOI:
10.1016/j.jneb.2019.11.014

2. Emerging Principles of Selective ER Autophagy

Author:
Wilkinson, Simon
Source:
Journal of molecular biology 2020 v.432 no.1 pp. 185-205
ISSN:
0022-2836
Subject:
metabolism,   etc   ; autophagy; endoplasmic reticulum; lysosomes; signal transduction;     Show all 5 Subjects
Abstract:
... The endoplasmic reticulum (ER) is a fundamental organelle in cellular metabolism and signal transduction. It is subject to complex, dynamic sculpting of morphology and composition. Degradation of ER content has an important role to play here. Indeed, a major emerging player in ER turnover is ER-phagy, the degradation of ER fragments by selective autophagy, particularly macroautophagy. This article ...
DOI:
10.1016/j.jmb.2019.05.012
PubMed:
31100386
PubMed Central:
PMC6971691

3. Identity and functions of inorganic and inositol polyphosphates in plants

Author:
Lorenzo‐Orts, Laura; Couto, Daniel; Hothorn, Michael
Source:
Thenew phytologist 2020 v.225 no.2 pp. 637-652
ISSN:
0028-646X
Subject:
metabolism,   etc   ; energy; enzymes; inositols; metabolites; pyrophosphates;     Show all 6 Subjects
Abstract:
... Inorganic polyphosphates (polyPs) and inositol pyrophosphates (PP‐InsPs) form important stores of inorganic phosphate and can act as energy metabolites and signaling molecules. Here we review our current understanding of polyP and inositol phosphate (InsP) metabolism and physiology in plants. We outline methods for polyP and InsP detection, discuss the known plant enzymes involved in their synthes ...
DOI:
10.1111/nph.16129
PubMed:
31423587
PubMed Central:
PMC6973038

4. A label-free shotgun proteomics analysis of macadamia nut

Author:
Rost, Johanna; Muralidharan, Sridevi; Lee, N. Alice
Source:
Food research international 2020 v.129 pp. 108838
ISSN:
0963-9969
Subject:
amino acid metabolism; lipid metabolism,   etc   ; allergens; antimicrobial peptides; macadamia nuts; proteomics; stress response; vicilin;     Show all 8 Subjects
Abstract:
... In this study, we present a systematic proteomic overview of macadamia nut using a label-free shotgun proteomic approach. We identified 947 proteins in 723 clusters and gene ontology analysis revealed proteins across 46 functional categories including carbohydrate metabolism (10%), protein metabolic processes (5%), amino acid metabolism (4%), transport (4%), stress response (3%), lipid metabolism ...
DOI:
10.1016/j.foodres.2019.108838

5. Integrated metabolomic analysis for intervention effects of Gushudan on glucocorticoid-induced osteoporostic rat plasma based on RP/HILIC-UHPLC-Q-Orbitrap HRMS

Author:
Yuan, Xuemei; Wen, Jing; Jia, Huiwen; Tong, Lin; Zhao, Jing; Zhao, Longshan; Xiong, Zhili
Source:
Analytical biochemistry 2020 v.591 pp. 113559
ISSN:
0003-2697
Subject:
amino acid metabolism; energy metabolism; lipid metabolism,   etc   ; Oriental traditional medicine; biochemical pathways; biomarkers; glycocholic acid; intestinal microorganisms; mass spectrometry; metabolites; metabolomics; models; osteoporosis; rats;     Show all 14 Subjects
Abstract:
... An integrated metabolomic strategy based on both RP-UHPLC-Q-Orbitrap HRMS and HILIC-UHPLC-Q-Orbitrap HRMS in rat plasma was developed and validated, to further understand the anti-osteoporosis effect of Gushudan (GSD) and its mechanism on glucocorticoid-induced osteoporosis (GIOP) rats in this study. The metabolites were separated and identified on C18 column (100 mm × 2.1 mm, 1.7 μm) and Amide co ...
DOI:
10.1016/j.ab.2019.113559

6. Anodic metabolic activity regulates the desalination efficiency in microbial catalysed electrochemical system

Author:
Vanitha, T.K.; Hemalatha, Manupati; Venkata Mohan, S.
Source:
Bioresource technology 2020 v.309 pp. 123334
ISSN:
0960-8524
Subject:
metabolism,   etc   ; biochemical pathways; catalytic activity; chemical oxygen demand; desalination; electrochemistry;     Show all 6 Subjects
Abstract:
... Anodic metabolic rate showed regulatory influence on the desalination performance of microbial desalination cell (MDC) under open (OC) and closed circuit (CC) operations. In this study, three MDCs were tested for desalination with three different organic substrate loads 1500 ± 55 mg/L in MDC-A; 3500 ± 10 g/L in MDC-B; 4500 ± 12 g/L in MDC-C. Higher desalination and substrate removal rates were obs ...
DOI:
10.1016/j.biortech.2020.123334

7. Proteomic study of Enterococcus durans LAB18S growing on prebiotic oligosaccharides

Author:
Comerlato, Carolina Baldisserotto; Ritter, Ana Carolina; Miyamoto, Kendi Nishino; Brandelli, Adriano
Source:
Food microbiology 2020 v.89 pp. 103430
ISSN:
0740-0020
Subject:
protein metabolism,   etc   ; Enterococcus durans; algorithms; oligosaccharides; prebiotics; probiotics; proteins; proteomics;     Show all 8 Subjects
Abstract:
... This study evaluates the influence of prebiotic carbohydrates, namely fructooligosaccharides (FOS) and galactooligosaccharides (GOS), on the protein expression of Enterococcus durans LAB18S. The strain was cultivated in 10 g L⁻¹ FOS, GOS or glucose (control) and cellular proteins were extracted for mass spectrometry analysis. A total of 771 proteins were identified and 135 E. durans proteins were ...
DOI:
10.1016/j.fm.2020.103430

8. Tracing metabolic flux through time and space with isotope labeling experiments

Author:
Allen, Doug K; Young, Jamey D
Source:
Current opinion in biotechnology 2020 v.64 pp. 92-100
ISSN:
0958-1669
Subject:
metabolism,   etc   ; biochemical pathways; isotope labeling; metabolites; phenotype; surveys;     Show all 6 Subjects
Abstract:
... Metabolism is dynamic and must function in context-specific ways to adjust to changes in the surrounding cellular and ecological environment. When isotopic tracers are used, metabolite flow (i.e. metabolic flux) can be quantified through biochemical networks to assess metabolic pathway operation. The cellular activities considered across multiple tissues and organs result in the observed phenotype ...
DOI:
10.1016/j.copbio.2019.11.003

9. Synthetic Biochemistry: The Bio-inspired Cell-Free Approach to Commodity Chemical Production

Author:
Bowie, James U.; Sherkhanov, Saken; Korman, Tyler P.; Valliere, Meaghan A.; Opgenorth, Paul H.; Liu, Hongjiang
Source:
Trends in biotechnology 2020 v.38 no.7 pp. 766-778
ISSN:
0167-7799
Subject:
metabolism,   etc   ; biochemical pathways; biomass; enzyme activity; manufacturing; metabolic engineering; mixing;     Show all 7 Subjects
Abstract:
... Metabolic engineering efforts that harness living organisms to produce natural products and other useful chemicals face inherent difficulties because the maintenance of life processes often runs counter to our desire to maximize important production metrics. These challenges are particularly problematic for commodity chemical manufacturing where cost is critical. A cell-free approach, where bioche ...
DOI:
10.1016/j.tibtech.2019.12.024

10. Algicidal activity recovery by a Li-doped up-conversion material converting visible light into UV

Author:
Sun, Pengfei; Zhao, Jingyu; Tang, Jun; Li, Lin; Wong, Po Keung; Song, Lirong; Wu, Yonghong
Source:
The Science of the total environment 2020 v.720 pp. 137596
ISSN:
0048-9697
Subject:
metabolism,   etc   ; Citrobacter; algicides; bacteria; light; mutation; secretion; wavelengths;     Show all 8 Subjects
Abstract:
... Citrobacter sp. R1, a highly efficient algicidal bacterium, undergoes a serious degeneration after long-term preservation. Here, we prepared a Li-doped up-conversion material ([Y₂SiO₅; Li (1%)]) to recover the degenerated Strain R1. As a result, the [Y₂SiO₅; Li (1%)] successfully enhanced the maximum algicidal activity of Strain R1 from 43.2 ± 1.2 to 72.5 ± 1.3%. In terms of recovery mechanisms, 1 ...
DOI:
10.1016/j.scitotenv.2020.137596

11. Interactions of dietary fat with the gut microbiota: Evaluation of mechanisms and metabolic consequences

Author:
Mokkala, Kati; Houttu, Noora; Cansev, Tuğçe; Laitinen, Kirsi
Source:
Clinical nutrition 2020 v.39 no.4 pp. 994-1018
ISSN:
0261-5614
Subject:
metabolism,   etc   ; dietary fat; homeostasis; humans; intestinal microorganisms; planning;     Show all 6 Subjects
Abstract:
... The current scientific literature proposes that both the amount and type of dietary fat modulate homeostasis of the gut microbiota; disturbances in homeostasis may have metabolic consequences with potentially serious clinical manifestations. The evidence for interactions between dietary fat and gut microbiota has been mostly derived from animal studies, but there is now also evidence emerging from ...
DOI:
10.1016/j.clnu.2019.05.003

12. Patterns of partnership: surveillance and mimicry in host-microbiota mutualisms

Author:
Wiles, Travis J; Guillemin, Karen
Source:
Current opinion in microbiology 2020 v.54 pp. 87-94
ISSN:
1369-5274
Subject:
metabolism,   etc   ; microbial activity; microbial communities; microorganisms; monitoring; mutualism; pathogens;     Show all 7 Subjects
Abstract:
... The repertoire of microbial cues monitored by animal and plant tissues encompasses not just molecules but also microbial activities. These include typical pathogen strategies of injuring membranes, degrading cellular material, and scavenging resources. These activities, however, are not exclusive to pathogens. Instead, they characterize the competitive strategies of microbes living in multispecies ...
DOI:
10.1016/j.mib.2020.01.012

13. Personality- and size-related metabolic performance in invasive round goby (Neogobius melanostomus)

Author:
Behrens, Jane W.; von Friesen, Lisa W.; Brodin, Tomas; Ericsson, Philip; Hirsch, Philipp Emanuel; Persson, Anders; Sundelin, Anna; van Deurs, Mikael; Nilsson, P. Anders
Source:
Physiology & behavior 2020 v.215 pp. 112777
ISSN:
0031-9384
Subject:
metabolism,   etc   ; Neogobius melanostomus; fish; freezing; invasive species; predation; prices; risk;     Show all 8 Subjects
Abstract:
... Differences between individuals in behavioral type (i.e. animal personality) are ecologically and evolutionarily important because they can have significant effects on fitness components such as growth and predation risk. In the present study we are used the invasive round goby (Neogobius melanostomus) from an established population in controlled experiments to examine the relationships among pers ...
DOI:
10.1016/j.physbeh.2019.112777

14. Eating Away at ROS to Regulate Stomatal Opening

Author:
Medeiros, David B.; Barros, Jessica A.S.; Fernie, Alisdair R.; Araújo, Wagner L.
Source:
Trends in plant science 2020 v.25 no.3 pp. 220-223
ISSN:
1360-1385
Subject:
metabolism,   etc   ; autophagy; guard cells; homeostasis; reactive oxygen species; stomatal movement;     Show all 6 Subjects
Abstract:
... Although reactive oxygen species (ROS) function in guard cell signaling has been demonstrated, the control of ROS homeostasis remains elusive. Recent findings point to multiple mechanisms controlling ROS levels in guard cells. These mechanisms require secondary metabolism and autophagy, providing the guard cells with a degree of plasticity during stomatal movements. ...
DOI:
10.1016/j.tplants.2019.12.023

15. Nrf2 in liver toxicology

Author:
Taguchi, Keiko; Kensler, Thomas W.
Source:
Archives of pharmacal research 2020 v.43 no.3 pp. 337-349
ISSN:
0253-6269
Subject:
metabolism,   etc   ; drugs; genes; hepatotoxicity; liver; mice; toxicology; toxins; transcription factors;     Show all 9 Subjects
Abstract:
... Liver plays essential roles in the metabolism of many endogenous chemicals and exogenous toxicants. Mechanistic studies in liver have been at the forefront of efforts to probe the roles of bioactivation and detoxication of environmental toxins and toxicants in hepatotoxicity. Moreover, idiosyncratic hepatoxicity remains a key barrier in the clinical development of drugs. The now vast Nrf2 field em ...
DOI:
10.1007/s12272-019-01192-3
PubMed:
31782059
PubMed Central:
PMC7182086

16. Light-Up Lipid Droplets Dynamic Behaviors Using a Red-Emitting Fluorogenic Probe

Author:
Zhang, Xin; Yuan, Lin; Jiang, Jianxia; Hu, Jiwen; du Rietz, Anna; Cao, Hongzhi; Zhang, Ruilong; Tian, Xiaohe; Zhang, Fengling; Ma, Yuguang; Zhang, Zhongping; Uvdal, Kajsa; Hu, Zhangjun
Source:
Analytical chemistry 2020 v.92 no.5 pp. 3613-3619
ISSN:
1520-6882
Subject:
lipid metabolism,   etc   ; droplets; fluorescent dyes; image analysis; lipids; lipotoxicity; photobleaching;     Show all 7 Subjects
Abstract:
... Intracellular lipid metabolism occurs in lipid droplets (LDs), which is critical to the survival of cells. Imaging LDs is an intuitive way to understand their physiology in live cells. However, this is limited by the availability of specific probes that can properly visualize LDs in vivo. Here, an LDs-specific red-emitting probe is proposed to address this need, which is not merely with an ultrahi ...
DOI:
10.1021/acs.analchem.9b04410

17. Energetic constraints on mammalian home‐range size

Author:
Boratyński, Zbyszek
Source:
Functional ecology 2020 v.34 no.2 pp. 468-474
ISSN:
0269-8463
Subject:
metabolism,   etc   ; basal metabolic rate; habitats; home range; mammals; phylogeny;     Show all 6 Subjects
Abstract:
... The metabolic theory of ecology predicts that rates of metabolic processes play a central role in constraining important fitness traits such as home‐range size. Locomotory behaviours are physiologically expensive, yet the relative importance of the animal's energetics in determining their home ranges is unknown. It is tested here whether maintenance costs (BMR; basal metabolic rate), energetic cap ...
DOI:
10.1111/1365-2435.13480

18. Highly efficient anaerobic co-degradation of complex persistent polycyclic aromatic hydrocarbons by a bioelectrochemical system

Author:
Zhou, Yukang; Zou, Qingping; Fan, Mengjie; Xu, Yuan; Chen, Yingwen
Source:
Journal of hazardous materials 2020 v.381 pp. 120945
ISSN:
0304-3894
Subject:
metabolism,   etc   ; anodes; biodegradation; bioelectrochemistry; ecosystems; microbial communities; phenanthrenes; toxicity;     Show all 8 Subjects
Abstract:
... Polycyclic aromatic hydrocarbons (PAHs) that undergo long-distance migration and have strong biological toxicity are a great threat to the health of ecosystems. In this study, the biodegradation characteristics and combined effects of mixed PAHs in bioelectrochemical systems (BESs) were studied. The results showed that, compared with a mono-carbon source, low-molecular-weight PAHs (LMW PAHs)-napht ...
DOI:
10.1016/j.jhazmat.2019.120945

19. Sequence, structure and function‐based classification of the broadly conserved FAH superfamily reveals two distinct fumarylpyruvate hydrolase subfamilies

Author:
Hong, Hwaseok; Seo, Hogyun; Park, Woojin; Kim, Kyung‐Jin
Source:
Environmental microbiology 2020 v.22 no.1 pp. 270-285
ISSN:
1462-2912
Subject:
metabolism,   etc   ; bioinformatics; crystal structure; hydrolases; phylogeny; proteins; statistical analysis;     Show all 7 Subjects
Abstract:
... Fumarylacetoacetate hydrolase (FAH) superfamily proteins are found ubiquitously in microbial pathways involved in the catabolism of aromatic substances. Although extensive bioinformatic data on these proteins have been acquired, confusion caused by problems with the annotation of these proteins hinders research into determining their physiological functions. Here we classify 606 FAH superfamily pr ...
DOI:
10.1111/1462-2920.14844

20. Specialized ER membrane domains for lipid metabolism and transport

Author:
Nishimura, Taki; Stefan, Christopher J.
Source:
Biochimica et biophysica acta 2020 v.1865 no.1 pp. 158492
ISSN:
1388-1981
Subject:
lipid metabolism,   etc   ; biosynthesis; endoplasmic reticulum; enzymes; lipid transfer proteins; lipids;     Show all 6 Subjects
Abstract:
... The endoplasmic reticulum (ER) is a highly organized organelle that performs vital functions including de novo membrane lipid synthesis and transport. Accordingly, numerous lipid biosynthesis enzymes are localized in the ER membrane. However, it is now evident that lipid metabolism is sub-compartmentalized within the ER and that lipid biosynthetic enzymes engage with lipid transfer proteins (LTPs) ...
DOI:
10.1016/j.bbalip.2019.07.001