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You searched for: Subject "methyltransferases" Remove constraint Subject: "methyltransferases"
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1. Electrochemiluminescence biosensor for DNA adenine methylation methyltransferase based on CRISPR/Cas12a trans-cleavage-induced dual signal enhancement

Author:
Cuiying Lin; Qingqing Huang; Mengjian Tian; Fang Luo; Jian Wang; Bin Qiu; Shuofei Yang; Zhenyu Lin
Source:
Talanta 2023 v.251 pp. 123748
ISSN:
0039-9140
Subject:
methyltransferases,   etc   ; DNA; adenine; biosensors; blood serum; detection limit; electrochemiluminescence; electrodes; ferrocenes; indium; methylation; restriction endonucleases; tin;     Show all 13 Subjects
Abstract:
... In this work, an electrochemiluminescence (ECL) biosensor with dual signal enhancement was constructed and used for DNA adenine methylation methyltransferase (Dam MTase) detection. At present of Dam MTase, restriction endonuclease (DPnI) cleaves hairpin DNA (HP) and releases the HP stem end as a single strand that can activate CRISPR/Cas12a trans-cleavage activity. Assisted by trans-cleavage, the ...
DOI:
10.1016/j.talanta.2022.123748

2. Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis

Author:
Wenqi Xu; Chenxi He; Emily G. Kaye; Jiahui Li; Mandi Mu; Geoffrey M. Nelson; Li Dong; Jiahua Wang; Feizhen Wu; Yujiang Geno Shi; Karen Adelman; Fei Lan; Yang Shi; Hongjie Shen
Source:
Molecular cell 2022 v.82 no.6 pp. 1156-1168.e7
ISSN:
1097-2765
Subject:
methyltransferases,   etc   ; genes; methylation;     Show all 3 Subjects
Abstract:
... N⁶-methyladenosine (m⁶A) methylation is co-transcriptionally deposited on mRNA, but a possible role of m⁶A on transcription remains poorly understood. Here, we demonstrate that the METTL3/METTL14/WTAP m⁶A methyltransferase complex (MTC) is localized to many promoters and enhancers and deposits the m⁶A modification on nascent transcripts, including pre-mRNAs, promoter upstream transcripts (PROMPTs) ...
DOI:
10.1016/j.molcel.2022.02.006

3. Comparative Analysis of Sulfonium−π, Ammonium−π, and Sulfur−π Interactions and Relevance to SAM-Dependent Methyltransferases

Author:
Katherine I. Albanese; Andrew Leaver-Fay; Joseph W. Treacy; Rodney Park; K. N. Houk; Brian Kuhlman; Marcey L. Waters
Source:
Journal of the American Chemical Society 2022 v.144 no.6 pp. 2535-2545
ISSN:
1520-5126
Subject:
methyltransferases,   etc   ; ammonium; glyphosate-trimesium; peptides; solvation; sulfur;     Show all 6 Subjects
Abstract:
... We report the measurement and analysis of sulfonium−π, thioether−π, and ammonium−π interactions in a β-hairpin peptide model system, coupled with computational investigation and PDB analysis. These studies indicated that the sulfonium−π interaction is the strongest and that polarizability contributes to the stronger interaction with sulfonium relative to ammonium. Computational studies demonstrate ...
DOI:
10.1021/jacs.1c09902

4. Diversification of Chemical Structures of Methoxylated Flavonoids and Genes Encoding Flavonoid-O-Methyltransferases

Author:
Yuting Liu; Alisdair R. Fernie; Takayuki Tohge
Source:
Plants 2022 v.11 no.4 pp. -
ISSN:
2223-7747
Subject:
methyltransferases,   etc   ; flavonols; functional diversity; lignin; metabolites; physiology;     Show all 6 Subjects
Abstract:
... The O-methylation of specialized metabolites in plants is a unique decoration that provides structural and functional diversity of the metabolites with changes in chemical properties and intracellular localizations. The O-methylation of flavonoids, which is a class of plant specialized metabolites, promotes their antimicrobial activities and liposolubility. Flavonoid O-methyltransferases (FOMTs), ...
DOI:
10.3390/plants11040564

5. An outbreak sustained by ST15 Klebsiella pneumoniae carrying 16S rRNA methyltransferases and blaNDM: evaluation of the global dissemination of these resistance determinants

Author:
Federica Sacco; Giammarco Raponi; Alessandra Oliva; Giulia Bibbolino; Vera Mauro; Federica Maria Di Lella; Lorenzo Volpicelli; Guido Antonelli; Mario Venditti; Alessandra Carattoli; Gabriele Arcari
Source:
International journal of antimicrobial agents 2022 v.60 no.2 pp. 106615
ISSN:
0924-8579
Subject:
methyltransferases,   etc   ; Klebsiella pneumoniae; aminoglycosides; genes; genomics; plasmids;     Show all 6 Subjects
Abstract:
... The spread of extremely-drug-resistant Klebsiella pneumoniae has become a major health threat worldwide. This is largely mediated by certain lineages, recognized as high-risk clones dispersed throughout the world. Analysis of an outbreak of nine ST15, NDM-1 metallo-β-lactamase-producing K. pneumoniae was performed. An IncC plasmid carrying the blaNDM₋₁ gene also carried the rare rmtC gene, encodin ...
DOI:
10.1016/j.ijantimicag.2022.106615

6. Advanced biotechnology using methyltransferase and its applications in bacteria: a mini review

Author:
Jun Ren; Hyang-Mi Lee; JunHao Shen; Dokyun Na
Source:
Biotechnology letters 2022 v.44 no.1 pp. 33-44
ISSN:
0141-5492
Subject:
methyltransferases,   etc   ; biotechnology; plasmids; prokaryotic cells; restriction endonucleases;     Show all 5 Subjects
Abstract:
... Since prokaryotic restriction-modification (RM) systems protect the host by cleaving foreign DNA by restriction endonucleases, it is difficult to introduce engineered plasmid DNAs into newly isolated microorganisms whose RM system is not discovered. The prokaryotes also possess methyltransferases to protect their own DNA from the endonucleases. As those methyltransferases can be utilized to methyl ...
DOI:
10.1007/s10529-021-03208-9

7. Deficiency in FTSJ1 Affects Neuronal Plasticity in the Hippocampal Formation of Mice

Author:
Viola von Bohlen und Halbach; Simone Venz; Simon Nwakor; Christian Hentschker; Elke Hammer; Heike Junker; Andreas W. Kuss; Oliver von Bohlen und Halbach; Lars R. Jensen
Source:
Biology 2022 v.11 no.7 pp. -
ISSN:
2079-7737
Subject:
methyltransferases,   etc   ; dendrites; hippocampus; mass spectrometry; memory; mice; neuroplasticity;     Show all 7 Subjects
Abstract:
... The role of the tRNA methyltransferase FTSJ1 in the brain is largely unknown. We analyzed whether FTSJ1-deficient mice (KO) displayed altered neuronal plasticity. We explored open field behavior (10 KO mice (aged 22–25 weeks)) and 11 age-matched control littermates (WT) and examined mean layer thickness (7 KO; 6 WT) and dendritic spines (5 KO; 5 WT) in the hippocampal area CA1 and the dentate gyru ...
DOI:
10.3390/biology11071011

8. Structural features of a minimal intact methyltransferase of a type I restriction-modification system

Author:
Pil-Won Seo; Andreas Hofmann; Jun-Ha Kim; Seung-A Hwangbo; Jun-Hong Kim; Ji-Won Kim; Thi Yen Ly Huynh; Hyon E. Choy; Soo-Jung Kim; Jimin Lee; Jie-Oh Lee; Kyeong Sik Jin; Suk-Youl Park; Jeong-Sun Kim
Source:
International journal of biological macromolecules 2022 v.208 pp. 381-389
ISSN:
0141-8130
Subject:
methyltransferases,   etc   ; DNA; Vibrio vulnificus; crystal structure; methylation; tail;     Show all 6 Subjects
Abstract:
... Type I restriction-modification enzymes are oligomeric proteins composed of methylation (M), DNA sequence-recognition (S), and restriction (R) subunits. The different bipartite DNA sequences of 2–4 consecutive bases are recognized by two discerned target recognition domains (TRDs) located at the two-helix bundle of the two conserved regions (CRs). Two M-subunits and a single S-subunit form an olig ...
DOI:
10.1016/j.ijbiomac.2022.03.115

9. A feedback regulation between ARF7‐mediated auxin signaling and auxin homeostasis involving MES17 affects plant gravitropism

Author:
Feng Zhang; Cuiling Li; Xingzhen Qu; Jiajia Liu; Zipeng Yu; Junxia Wang; Jiayong Zhu; Yongqiang Yu; Zhaojun Ding
Source:
Journal of integrative plant biology 2022 v.64 no.7 pp. 1339-1351
ISSN:
1672-9072
Subject:
methyltransferases,   etc   ; gravitropism; gravity; homeostasis; hypocotyls; indole acetic acid;     Show all 6 Subjects
Abstract:
... Gravitropism is an essential adaptive response of land plants. Asymmetric auxin gradients across plant organs, interpreted by multiple auxin signaling components including AUXIN RESPONSE FACTOR7 (ARF7), trigger differential growth and bending response. However, how this fundamental process is strictly maintained in nature remains unclear. Here, we report that gravity stimulates the transcription o ...
DOI:
10.1111/jipb.13268

10. Targeting Viral Methyltransferases: An Approach to Antiviral Treatment for ssRNA Viruses

Author:
Peter Ramdhan; Chenglong Li
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
methyltransferases,   etc   ; RNA; catalytic activity; glycine (amino acid); innate immunity;     Show all 5 Subjects
Abstract:
... Methyltransferase enzymes have been associated with different processes within cells and viruses. Specifically, within viruses, methyltransferases are used to form the 5′cap-0 structure for optimal evasion of the host innate immune system. In this paper, we seek to discuss the various methyltransferases that exist within single-stranded RNA (ssRNA) viruses along with their respective inhibitors. A ...
DOI:
10.3390/v14020379

11. Molecular and biochemical characterization of Catharanthus roseus perivine-Nβ-methyltransferase

Author:
Dylan Levac; Paulo Cázares Flores; Vincenzo De Luca
Source:
Phytochemistry 2022 v.201 pp. 113266
ISSN:
0031-9422
Subject:
methyltransferases,   etc   ; Catharanthus roseus; biosynthesis; genes; monoterpenoids; phytochemicals; substrate specificity;     Show all 7 Subjects
Abstract:
... The biosynthesis of monoterpenoid indole alkaloids in Catharanthus roseus has been most extensively studied, leading to the detailed characterization of the pathway for the formation of their well-known anticancer alkaloids. The present study describes the identification, molecular cloning, and functional expression of C. roseus perivine-Nᵦ-methyltransferase (PeNMT) that converts perivine to Nᵦ-me ...
DOI:
10.1016/j.phytochem.2022.113266

12. N6-methyladenosine RNA modification of glutamatergic neurons is associated with contextual fear discrimination

Author:
Yuan Chang; Jinming Zhang; Junmin Zhang; Wenpeng Zhu; Qiaohua Zheng; Zhaoqiang Qian; Chunling Wei; Yihui Liu; Zhiqiang Liu; Wei Ren; Jing Han
Source:
Physiology & behavior 2022 v.248 pp. 113741
ISSN:
0031-9384
Subject:
methyltransferases,   etc   ; RNA; astrocytes; brain; fearfulness; memory; methylation; mice; neurophysiology;     Show all 9 Subjects
Abstract:
... Fear memory overgeneralization is a hallmark of many stress-related disorders, especially posttraumatic stress disorder. The neurobiology of fear memory generalization and discrimination involves a series of interplays between molecular and cellular factors, the mechanisms of which remain largely unexplored. N⁶-methyladenosine (m⁶A) of RNA is a reversible and dynamically regulated posttranscriptio ...
DOI:
10.1016/j.physbeh.2022.113741

13. NSD1 mediates antagonism between SWI/SNF and polycomb complexes and is required for transcriptional activation upon EZH2 inhibition

Author:
Yiannis Drosos; Jacquelyn A. Myers; Beisi Xu; Kaeli M. Mathias; Emma C. Beane; Sandi Radko-Juettner; Robert J. Mobley; Margaret E. Larsen; Federica Piccioni; Xiaotu Ma; Jonathan Low; Baranda S. Hansen; Samuel T. Peters; Natarajan V. Bhanu; Sandeep K. Dhanda; Taosheng Chen; Santhosh A. Upadhyaya; Shondra M. Pruett-Miller; David E. Root; Benjamin A. Garcia; Janet F. Partridge; Charles W.M. Roberts
Source:
Molecular cell 2022 v.82 no.13 pp. 2472-2489.e8
ISSN:
1097-2765
Subject:
methyltransferases,   etc   ; antagonism; chromatin; mutants; sarcoma; transcription (genetics); transcriptional activation;     Show all 7 Subjects
Abstract:
... Disruption of antagonism between SWI/SNF chromatin remodelers and polycomb repressor complexes drives the formation of numerous cancer types. Recently, an inhibitor of the polycomb protein EZH2 was approved for the treatment of a sarcoma mutant in the SWI/SNF subunit SMARCB1, but resistance occurs. Here, we performed CRISPR screens in SMARCB1-mutant rhabdoid tumor cells to identify genetic contrib ...
DOI:
10.1016/j.molcel.2022.04.015

14. Quantification of mRNA cap-modifications by means of LC-QqQ-MS

Author:
Nils Muthmann; Petr Špaček; Dennis Reichert; Melissa van Dülmen; Andrea Rentmeister
Source:
Methods 2022 v.203 pp. 196-206
ISSN:
1046-2023
Subject:
methyltransferases,   etc   ; S-adenosylmethionine; immunogenicity; liquid chromatography; tandem mass spectrometry;     Show all 5 Subjects
Abstract:
... Enzymatic modification of the 5′-cap is a versatile approach to modulate the properties of mRNAs. Transfer of methyl groups from S-adenosyl-l-methionine (AdoMet) or functional moieties from non-natural analogs by methyltransferases (MTases) allows for site-specific modifications at the cap. These modifications have been used to tune translation or control it in a temporal manner and even influence ...
DOI:
10.1016/j.ymeth.2021.05.018

15. Biosynthetic Pathway Construction and Production Enhancement of 1-Hydroxyphenazine Derivatives in Pseudomonas chlororaphis H18

Author:
Yupeng Wan; Hongchen Liu; Mo Xian; Wei Huang
Source:
Journal of agricultural and food chemistry 2022 v.70 no.4 pp. 1223-1231
ISSN:
1520-5118
Subject:
methyltransferases,   etc   ; Pseudomonas chlororaphis; biochemical pathways; biosynthesis; food chemistry; methylation; phenazines;     Show all 7 Subjects
Abstract:
... 1-Hydroxyphenazine derivatives are phenazine family chemicals with broad-spectrum antibacterial and potential biological activities. However, the lack of variety and low titer hinder their applications. In this research, three enzymes PhzS (monooxygenase), NaphzNO1 (N-monooxygenase), and LaphzM (methyltransferase) were heterologously expressed in a phenazine-1-carboxylic acid generating strain Pse ...
DOI:
10.1021/acs.jafc.1c07760

16. Molecular Networking-Based Screening Led to the Discovery of a Cyclic Heptadepsipeptide from an Endolichenic Xylaria sp.

Author:
Mengna Luo; Shanshan Chang; Yihong Li; Xiaoming Xi; Minghua Chen; Ning He; Mengyuan Wang; Wuli Zhao; Yunying Xie
Source:
Journal of natural products 2022 v.85 no.4 pp. 972-979
ISSN:
1520-6025
Subject:
methyltransferases,   etc   ; Xylaria; biochemical pathways; bioinformatics; depsipeptides; hydrolysis; neoplasm cells; prioritization;     Show all 8 Subjects
Abstract:
... MS/MS-based molecular networking strain prioritization led to the discovery of a group of cyclic depsipeptides from an endolichenic Xylaria sp. The main component, xylaroamide A (1), was obtained by LC-MS-guided isolation. The planar structure of compound 1 was elucidated via 1D and 2D NMR, as well as MS/MS data. The configurations were fully determined by the combination of advanced Marfey’s anal ...
DOI:
10.1021/acs.jnatprod.1c01108

17. Structure and mechanism of histone methylation dynamics in Arabidopsis

Author:
Hongmiao Hu; Jiamu Du
Source:
Current opinion in plant biology 2022 v.67 pp. 102211
ISSN:
1369-5266
Subject:
methyltransferases,   etc   ; Arabidopsis; chromatin; gene expression; histone demethylases; histones; methylation; plant biology;     Show all 8 Subjects
Abstract:
... Histone methylation plays a central role in regulating chromatin state and gene expression in Arabidopsis and is involved in a variety of physiological and developmental processes. Dynamic regulation of histone methylation relies on both histone methyltransferase “writer” and histone demethylases “eraser” proteins. In this review, we focus on the four major histone methylation modifications in Ara ...
DOI:
10.1016/j.pbi.2022.102211

18. A SA-regulated lincRNA promotes Arabidopsis disease resistance by modulating pre-rRNA processing

Author:
Xiaoyu Kong; Huacai Wang; Mengting Zhang; Xiaoying Chen; Rongxiang Fang; Yongsheng Yan
Source:
Plant science 2022 v.316 pp. 111178
ISSN:
0168-9452
Subject:
methyltransferases,   etc   ; Arabidopsis; biosynthesis; disease resistance; gene expression regulation; mutants; pathogens; ribosomes;     Show all 8 Subjects
Abstract:
... Regulation of gene expression at translational level has been shown critical for plant defense against pathogen infection. Pre-rRNA processing is essential for ribosome biosynthesis and thus affects protein translation. It remains unknown if plants modulate pre-rRNA processing as a translation regulatory mechanism for disease resistance. In this study, we show a 5' snoRNA capped and 3' polyadenyla ...
DOI:
10.1016/j.plantsci.2022.111178

19. FTO knockdown alleviates hypoxia-induced PC12 cell injury by stabilizing GADD45B in an IGF2BP2-dependent manner

Author:
Dan Wang; Yu Li; Xiaoxiao Xu; Shixin Zhao; Zhen Wang; Jiahao Yang; Xi Zhang; Junwei Pan; Yisheng Wang; Ming Liu
Source:
Biochemical and biophysical research communications 2022 v.619 pp. 166-172
ISSN:
0006-291X
Subject:
methyltransferases,   etc   ; RNA; animal injuries; hypoxia; models; neurodevelopment; obesity; research; spinal cord;     Show all 9 Subjects
Abstract:
... RNA N6-methyladenosine (m6A) level is closely associated with neurodevelopment and central nervous system dysfunctions including spinal cord injury (SCI). M6A level can be dynamically regulated by m6A methyltransferases and demethylases. In this text, the roles of m6A demethylase FTO alpha-ketoglutarate dependent dioxygenase (FTO) in SCI development along with its m6A-dependent regulatory mechanis ...
DOI:
10.1016/j.bbrc.2022.06.039

20. METTL14 gene polymorphisms influence hepatoblastoma predisposition in Chinese children: Evidences from a seven-center case-control study

Author:
Huitong Chen; Zhen Chen; Mi Wang; Jiao Zhang; Yong Li; Li Li; Suhong Li; Jiwen Cheng; Xianqiang Wang; Huimin Xia; Zhonghua Yang; Jing He
Source:
Gene 2022 v.809 pp. 146050
ISSN:
0378-1119
Subject:
methyltransferases,   etc   ; carcinogenesis; case-control studies; etiology; genes; genotype; genotyping; quantitative traits; risk;     Show all 9 Subjects
Abstract:
... Hepatoblastoma as the most prevalent hepatic malignancy in children, its etiology remains unclear. N⁶-Methyladenosine (m⁶A) modification which can modify various physiological processes, plays a critical role in tumorigenesis. Methyltransferase-like 14 (METTL14), an important component of the m⁶A methyltransferase complex, remains elusive during hepatoblastoma occurrence and development. We explor ...
DOI:
10.1016/j.gene.2021.146050