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microarraytechnology, etc ; T-lymphocytes; biomarkers; gene expression; genes; immunosuppression; immunosuppressive agents; kidney transplant; monitoring; Show all 9 Subjects
Abstract:
... Achieving spontaneous tolerance is the optimal goal in renal transplantation (RT). However, robust biomarkers indicating spontaneous tolerance are still lacking for RT recipients in clinics. The peripheral blood gene expression profiles of RT recipients in the state of tolerance and other conditions from four independent cohorts were collected in databases. Immune cell abundance assessment and sin ...
... Standardization has been a widely adopted practice in multiple testing, for it takes into account the variability in sampling and makes the test statistics comparable across different study units. However, despite conventional wisdom to the contrary, we show that there can be a significant loss in information from basing hypothesis tests on standardized statistics rather than the full data. We dev ...
microarraytechnology, etc ; DNA; humans; transcriptome; transcriptomics; Show all 5 Subjects
Abstract:
... High RNA integrity is essential for good quality of transcriptomics profiling. Nevertheless, in some cases samples with low RNA integrity is the only available material to study. This work was set to investigate the impact of thermal-induced RNA degradation on the transcriptomic profiles of human leukemic cells. DNA microarray-based transcriptomics was conducted on two groups of samples; high RNA ...
microarraytechnology, etc ; durability; mice; nanomaterials; polymers; Show all 5 Subjects
Abstract:
... Polymer micro/nanoarchitectures have attracted intense interest for wearable medical applications due to their excellent mechanical flexibility, solution processability, and tunable optoelectronic properties. Based on polymer micro/nanostructures, high-performance ultraviolet (UV) photodetectors can not only functionalize the accurate image sensing but also sustain the biocomfortable flexible devi ...
microarraytechnology, etc ; aflatoxin B1; biosensors; liquid crystals; Show all 4 Subjects
Abstract:
... Herein, a signal-on liquid crystal microarray (LCM) sensor is designed for the first time with a micro-spectral optical sensing signal. Depending on the change of the orientation of the LC molecules in the LCM films and the intensity of the spectral peaks of the PhCs, the signal-on LCM biosensor achieves the detection of AFB1 and the Partial Response Mechanism (PSM) of the LCM films is discovered. ...
microarraytechnology, etc ; breast neoplasms; carcinogenesis; metastasis; therapeutics; Show all 5 Subjects
Abstract:
... Triple-negative breast cancer (TNBC) has an aggressive biological behavior and poor outcome. Our published study showed that PAI-1 could induce the migration and metastasis of TNBC cells. However, the underlying mechanism by which PAI-1 regulates TNBC metastasis has not been addressed. Here, we demonstrated that PAI-1 is high expressed in TNBC and promotes TNBC cells tumorigenesis. Using microarra ...
microarraytechnology, etc ; cluster analysis; data collection; sequence analysis; Show all 4 Subjects
Abstract:
... Estimating the number of clusters (K) is a critical and often difficult task in cluster analysis. Many methods have been proposed to estimate K, including some top performers using resampling approach. When performing cluster analysis in high‐dimensional data, simultaneous clustering and feature selection is needed for improved interpretation and performance. To our knowledge, little has been stud ...
microarraytechnology, etc ; brain; chromatin; genes; histones; low calorie diet; microRNA; neurogenesis; Show all 8 Subjects
Abstract:
... Calorie restriction (CR) has been shown to be one of the most effective methods in alleviating the effects of ageing and age-related diseases. Although the protective effects of CR have been reported, the exact molecular mechanism still needs to be clarified. This study aims to determine differentially expressed (DE) miRNAs and altered gene pathways due to long-term chronic (CCR) and intermittent ...
microarraytechnology, etc ; biomarkers; gene expression; male sterility; microRNA; reproduction; testes; therapeutics; Show all 8 Subjects
Abstract:
... Male infertility is a multifactorial disorder that involves different physiopathological mechanisms and multiple genes. In this sense, we analyse the role of miRNAs in this pathology. Gene expression analysis can provide relevant information to detect biomarkers, signalling pathways, pathologic mechanisms, and potential therapeutic targets for the disease. In this review, we describe four miRNA mi ...
microarraytechnology, etc ; biosensors; dopamine; electrochemiluminescence; electrochemistry; hypoxia; oligonucleotides; oxidation; polymers; secretion; Show all 10 Subjects
Abstract:
... The quantitative detection of single cell secretions is always limited by their accurate collection and the heterogeneity of different cells. In this work, a confined electrochemiluminescence (ECL) imaging microarray (CEIM) chip was designed to capture single or a few cells in each cylindrical microwell for high-throughput quantitation of cell-secreted dopamine (DA). The ITO surface at the bottom ...
microarraytechnology, etc ; DNA; databases; forensic sciences; genome; heterozygosity; homozygosity; humans; Australia; Show all 9 Subjects
Abstract:
... The successful application of forensic genetic genealogy (FGG) to identify Jane and John Doe cases in the United States has raised the prospect of using the technique in Australia to assist in the reconciliation of unidentified human remains (UHRs) with long term missing persons. A study was conducted to explore the feasibility of FGG using whole genome array (WGA) data from both pristine control ...
microarraytechnology, etc ; adipocytes; gene expression regulation; lipids; research; transcription (genetics); transcriptomics; Show all 7 Subjects
Abstract:
... Long non-coding RNAs (lncRNAs) have gained extensive attentions due to their significant roles in diverse biological process. However, the potential functions of lncRNAs participation in adipocyte differentiation have not been fully explored. In the present study, we globally profiled lncRNA expression using lncRNA microarray and identified 1745 lncRNA probes with differential expression on day 0 ...
microarraytechnology, etc ; data collection; eukaryotic cells; genes; prediction; sequence analysis; transcriptomics; Show all 7 Subjects
Abstract:
... Gene coexpression analysis constitutes a widely used practice for gene partner identification and gene function prediction, consisting of many intricate procedures. The analysis begins with the collection of primary transcriptomic data and their preprocessing, continues with the calculation of the similarity between genes based on their expression values in the selected sample dataset and results ...
microarraytechnology, etc ; gene expression; horticulture; reverse transcriptase polymerase chain reaction; transcriptome; Show all 5 Subjects
Abstract:
... Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is extensively applied technique to investigate the transcript abundance of target genes in various organisms. Selection of appropriate reference genes (RGs) for qRT-PCR normalization is a crucial prerequisite for accurately quantifying gene expression level. RGs should exhibit minimal variation in gene expression. However, the ...
microarraytechnology, etc ; alleles; artificial selection; dissection; genomics; genotyping; prediction; progeny; soybeans; Show all 9 Subjects
Abstract:
... KEY MESSAGE: We developed the ZDX1 high-throughput functional soybean array for high accuracy evaluation and selection of both parents and progeny, which can greatly accelerate soybean breeding. Microarray technology facilitates rapid, accurate, and economical genotyping. Here, using resequencing data from 2214 representative soybean accessions, we developed the high-throughput functional array ZD ...
microarraytechnology, etc ; animals; glycomics; lectins; ligands; polysaccharides; quantitative analysis; rapid methods; Show all 8 Subjects
Abstract:
... Through their specific interactions with proteins, cellular glycans play key roles in a wide range of physiological and pathological processes. One of the main goals of research in the areas of glycobiology and glycomedicine is to understand glycan–protein interactions at the molecular level. Over the past two decades, glycan microarrays have become powerful tools for the rapid evaluation of inter ...
microarraytechnology, etc ; animals; antibody formation; globulins; monitoring; proteome; proteomics; smallpox; vaccines; Show all 9 Subjects
Abstract:
... The availability of effective smallpox vaccines was a critical element of the successful eradication of smallpox in 1980. Antibody responses play a primary role in protective immunity and neutralizing antibody is an established correlate of protection against smallpox. In this study we used a poxvirus proteome array to assess the antibody response to individual viral proteins in a cohort of 1,037 ...
microarraytechnology, etc ; biosensors; cell communication; interleukin-2; lymphoma; secretion; spectral analysis; Show all 7 Subjects
Abstract:
... Proteins secreted by cells play significant roles in mediating many physiological, developmental, and pathological processes due to their functions in intra/intercellular communication and signaling. Conventional end-point methods are insufficient for understanding the temporal response in cell secretion process, which is often highly dynamic. Furthermore, cellular heterogeneity makes it essential ...
microarraytechnology, etc ; angiogenesis; breast neoplasms; breasts; cell cycle; cell proliferation; metastasis; microvessels; Show all 8 Subjects
Abstract:
... As an indispensable process for breast cancer metastasis, tumour angiogenesis requires a tight interaction between cancer cells and endothelial cells in tumour microenvironment. Here, we explored the participation of small extracellular vesicles (sEVs) derived from breast cancer cells in modulating angiogenesis and investigated the effect of IL‐35 in facilitating this process. Firstly, we characte ...
microarraytechnology, etc ; colitis; colon; dextran sulfate; glucosylceramides; lymph; pathogenesis; research; spleen; therapeutics; Show all 10 Subjects
Abstract:
... Inflammatory bowel disease (IBD) is a chronic inflammatory disease in the colon characterized by excessive activation of T cells. Glycosphingolipids (GSLs) are composed of lipid rafts in cellular membranes, and their content is linked to immune cell function. In the present study, we investigated the involvement of GSLs in IBD. Microarray data showed that in IBD patients, the expression of only UD ...