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1. Revisiting Bartonella bacilliformis MLST

Author:
Ruiz, Joaquim; Pons, Maria J.
Source:
Infection, genetics, and evolution 2018 v.63 pp. 231-235
ISSN:
1567-1348
Subject:
microbial genetics,   etc   ; Bartonella bacilliformis; genome; geographical distribution;     Show all 4 Subjects
Abstract:
... All the studies published including Bartonella bacilliformis MLST data, as well as all B. bacilliformis genomes present in GenBank were analyzed. Overall 64 isolates and their geographical distribution were analyzed, and 14 different MLST patterns were observed. The results highlight the need for expanding the MLST studies and adding a higher number of isolates from all endemic areas. ...
DOI:
10.1016/j.meegid.2018.05.032

2. Testing sampling effort and relative abundance descriptors of belowground ectomycorrhizal fungi in a UK planted scots pine woodland

Author:
Villarreal-Ruiz, Luis; Neri-Luna, Cecilia
Source:
Mycology 2018 v.9 no.2 pp. 106-115
ISSN:
2150-1211
Subject:
microbial genetics,   etc   ; Cortinarius; Pinus sylvestris; biodiversity; ectomycorrhizae; fungal communities; mycorrhizal fungi; root tips; soil; woodlands; Scotland;     Show all 11 Subjects
Abstract:
... The native Caledonian pinewoods of Scotland, UK contain a unique and unexplored biodiversity of below-ground ectomycorrhizal fungi which may constitute a valuable source of microbial genetics resources for woodland restoration. In this study we test the sampling effort, taxa detection (ectomycorrhiza morpho-anatotyping) and relative abundance measurements (ectomycorrhizal root tip number vs. dry w ...
DOI:
10.1080/21501203.2017.1394393

3. Thinking Outside the Cell: Replicating Replication In Vitro

Author:
Aguilar, Rhiannon R.; Tyler, Jessica K.
Source:
Molecular cell 2017 v.65 no.1 pp. 5-7
ISSN:
1097-2765
Subject:
microbial genetics,   etc   ; DNA replication; Saccharomyces cerevisiae; chromatin; yeasts;     Show all 5 Subjects
Abstract:
... Benchmark studies by Yeeles et al. (2017), Kurat et al. (2017), and Devbhandari et al. (2017) have reconstituted rapid and regulated budding yeast DNA replication on naked and chromatinized templates in vitro, providing key insights into this fundamentally important process. ...
DOI:
10.1016/j.molcel.2016.12.019
PubMed:
28061333
PubMed Central:
PMC5859927

4. Co-circulation dynamics and persistence of newly introduced clades of 2012 outbreak associated West Nile Virus in Texas, 2012–2015

Author:
Aroh, Chukwuemika; Liang, Chaoying; Raj, Prithvi; Wakeland, Benjamin; Yan, Nan; Wakeland, Edward
Source:
Infection, genetics, and evolution 2018 v.66 pp. 13-17
ISSN:
1567-1348
Subject:
microbial genetics,   etc   ; Culicidae; West Nile fever; West Nile virus; genome; genotype; mutation; niches; overwintering; summer; New York; Texas;     Show all 12 Subjects
Abstract:
... The second largest outbreak of West Nile encephalitis and West Nile fever ever recorded occurred in the United States (U.S) in the summer of 2012. The outbreak was related to the widespread circulation of closely related clades, or groups, of West Nile virus (WNV) into multiple states where they were not previously found. Whether the invading 2012 strains were able to circulate and overwinter in s ...
DOI:
10.1016/j.meegid.2018.08.025

5. Regulations governing the multicellular lifestyle of Myxococcus xanthus

Author:
Mercier, Romain; Mignot, Tâm
Source:
Current opinion in microbiology 2016 v.34 pp. 104-110
ISSN:
1369-5274
Subject:
microbial genetics,   etc   ; Myxococcus xanthus; bacteria; bacterial motility; fruiting bodies; spores;     Show all 6 Subjects
Abstract:
... In living organisms, cooperative cell movements underlie the formation of differentiated tissues. In bacteria, Myxococcus xanthus uses cooperative group movements, to predate on prey and to form multicellular fruiting bodies, where the cells differentiate into dormant spores. Motility is controlled by a central signaling Che-like pathway, Frz. Single cell studies indicate Frz regulates the frequen ...
DOI:
10.1016/j.mib.2016.08.009

6. The quest for the minimal bacterial genome

Author:
Martínez-García, Esteban; de Lorenzo, Víctor
Source:
Current opinion in biotechnology 2016 v.42 pp. 216-224
ISSN:
0958-1669
Subject:
microbial genetics,   etc   ; DNA; bacteria; chromosomes; genes; nucleotide sequences;     Show all 6 Subjects
Abstract:
... The onset of techniques for both editing extant bacterial chromosomes and synthesizing long DNA sequences has enabled addressing the question on the smallest set of genes and biological functions that are required for running an operative cell. But this is not only a fundamental scientific endeavour: simpler genomes could be easier to understand and eventually reprogram for the sake of diverse app ...
DOI:
10.1016/j.copbio.2016.09.001

7. Network-Thinking: Graphs to Analyze Microbial Complexity and Evolution

Author:
Corel, Eduardo; Lopez, Philippe; Méheust, Raphaël; Bapteste, Eric
Source:
Trends in microbiology 2016 v.24 no.3 pp. 224-237
ISSN:
0966-842X
Subject:
microbial genetics,   etc   ; data collection; evolution; gene transfer; genes; microorganisms; models;     Show all 7 Subjects
Abstract:
... The tree model and tree-based methods have played a major, fruitful role in evolutionary studies. However, with the increasing realization of the quantitative and qualitative importance of reticulate evolutionary processes, affecting all levels of biological organization, complementary network-based models and methods are now flourishing, inviting evolutionary biology to experience a network-think ...
DOI:
10.1016/j.tim.2015.12.003
PubMed:
26774999
PubMed Central:
PMC4766943

8. A journey through the microscopic ages of DNA replication

Author:
Reinhart, Marius; Cardoso, M. Cristina
Source:
Protoplasma 2017 v.254 no.3 pp. 1151-1162
ISSN:
0033-183X
Subject:
microbial genetics,   etc   ; DNA; DNA replication; digital images; fluorescence microscopy; image analysis;     Show all 6 Subjects
Abstract:
... Scientific discoveries and technological advancements are inseparable but not always take place in a coherent chronological manner. In the next, we will provide a seemingly unconnected and serendipitous series of scientific facts that, in the whole, converged to unveil DNA and its duplication. We will not cover here the many and fundamental contributions from microbial genetics and in vitro bioche ...
DOI:
10.1007/s00709-016-1058-8
PubMed:
27943022
PubMed Central:
PMC5376393

9. A genetic screen for plant mutants with altered nodulation phenotypes in response to rhizobial glycan mutants

Author:
Liu, Huijun; Sandal, Niels; Andersen, Kasper R.; James, Euan K.; Stougaard, Jens; Kelly, Simon; Kawaharada, Yasuyuki
Source:
Thenew phytologist 2018 v.220 no.2 pp. 526-538
ISSN:
0028-646X
Subject:
microbial genetics,   etc   ; Lotus; Mesorhizobium loti; alanine; alleles; chromosome mapping; cytokinins; ethylene production; gain-of-function mutation; mutants; nitrogen fixation; nodulation; organogenesis; phenotype; plant response; polysaccharides; valine;     Show all 17 Subjects
Abstract:
... Nodule primordia induced by rhizobial glycan mutants often remain uninfected. To identify processes involved in infection and organogenesis we used forward genetics to identify plant genes involved in perception and responses to bacterial glycans. To dissect the mechanisms underlying the negative plant responses to the Mesorhizobium loti R7AexoU and ML001cep mutants, a screen for genetic suppresso ...
DOI:
10.1111/nph.15293

10. Our unique microbial identity

Author:
Gilbert, Jack A
Source:
GenomeBiology.com 2015 v.16 no.1 pp. 97
ISSN:
1465-6906
Subject:
microbial genetics,   etc   ; disease resistance; drug therapy; infectious diseases; microorganisms;     Show all 5 Subjects
Abstract:
... A recent article examines the extent of individual variation in microbial identities and how this might determine disease susceptibility, therapeutic responses and recovery from clinical interventions.Please see related article: http://dx.doi.org/10.1186/s13059-015-0646-9 ...
DOI:
10.1186/s13059-015-0664-7
PubMed:
25971745
PubMed Central:
PMC4430908

11. Rapid prototyping of microbial cell factories via genome-scale engineering

Author:
Si, Tong; Xiao, Han; Zhao, Huimin
Source:
Biotechnology Advances 2015 v.33 pp. 1420-1432
ISSN:
0734-9750
Subject:
microbial genetics,   etc   ; drugs; engineering; fuels; genome; genomics; microorganisms; technology;     Show all 8 Subjects
Abstract:
... Advances in reading, writing and editing genetic materials have greatly expanded our ability to reprogram biological systems at the resolution of a single nucleotide and on the scale of a whole genome. Such capacity has greatly accelerated the cycles of design, build and test to engineer microbes for efficient synthesis of fuels, chemicals and drugs. In this review, we summarize the emerging techn ...
DOI:
10.1016/j.biotechadv.2014.11.007
PubMed:
25450192
PubMed Central:
PMC4439387

12. TRAPPII regulates exocytic Golgi exit by mediating nucleotide exchange on the Ypt31 ortholog RabEᴿᴬᴮ¹¹

Author:
Pinar, Mario; Arst, Herbert N.; Pantazopoulou, Areti; Tagua, Víctor G.; de los Rííos, Vivian; Rodríííguez-Salarichs, Javier; Dííííaz, J. Fernando; Peííííñalva, Miguel A.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2015 v.112 no.14 pp. 4346-4351
ISSN:
0027-8424
Subject:
microbial genetics,   etc   ; Aspergillus; binding sites; guanosinetriphosphatase; molecular genetics; traffic;     Show all 6 Subjects
Abstract:
... The oligomeric complex transport protein particle I (TRAPPI) mediates nucleotide exchange on the RAB GTPase RAB1/Ypt1. TRAPPII is composed of TRAPPI plus three additional subunits, Trs120, Trs130, and Trs65. Unclear is whether TRAPPII mediates nucleotide exchange on RAB1/Ypt1, RAB11/Ypt31, or both. In Aspergillus nidulans , RabO ᴿᴬᴮ¹ resides in the Golgi, RabE ᴿᴬᴮ¹¹ localizes to exocytic post-Golg ...
DOI:
10.1073/pnas.1419168112
PubMed:
25831508
PubMed Central:
PMC4394270

13. Metabolic bacterial genes and the construction of high-level composite lineages of life

Author:
Méheust, Raphaël; Lopez, Philippe; Bapteste, Eric
Source:
Trends in ecology & evolution 2015 v.30 no.3 pp. 127-129
ISSN:
0169-5347
Subject:
microbial genetics,   etc   ; Archaea; evolution; gene transfer; genes; microbial ecology;     Show all 6 Subjects
Abstract:
... Understanding how major organismal lineages originated is fundamental for understanding processes by which life evolved. Major evolutionary transitions, like eukaryogenesis, merging genetic material from distantly related organisms, are rare events, hence difficult ones to explain causally. If most archaeal lineages emerged after massive acquisitions of bacterial genes, a rule however arises: meta ...
DOI:
10.1016/j.tree.2015.01.001
PubMed:
25601290
PubMed Central:
PMC4359277

14. The novel Aspergillus fumigatus MAT1-2-4 mating-type gene is required for mating and cleistothecia formation

Author:
Yu, Yidong; Amich, Jorge; Will, Cornelia; Eagle, Carly E.; Dyer, Paul S.; Krappmann, Sven
Source:
Fungal genetics and biology 2017 v.108 pp. 1-12
ISSN:
1087-1845
Subject:
microbial genetics,   etc   ; Aspergillus fumigatus; animal pathogens; bioinformatics; cleistothecia; gene expression; genes; heterokaryon; loci; sexual development; transcription factors;     Show all 11 Subjects
Abstract:
... Sexual propagation accompanied by recombination and the formation of spore-containing fruiting bodies is a cornerstone of fungal genetics and biology. In the human pathogen Aspergillus fumigatus sexual identity has previously been shown to be determined by MAT1-1-1 or MAT1-2-1 genes which act as transcriptional regulators and are present within idiomorphs found at the MAT locus. We here report the ...
DOI:
10.1016/j.fgb.2017.09.001

15. C. elegans and its bacterial diet as a model for systems-level understanding of host–microbiota interactions

Author:
Zhang, Jingyan; Holdorf, Amy D; Walhout, Albertha JM
Source:
Current opinion in biotechnology 2017 v.46 pp. 74-80
ISSN:
0958-1669
Subject:
microbial genetics,   etc   ; Caenorhabditis elegans; bacteria; diet; genes; human health; humans; intestinal microorganisms; life history; metabolism; models; screening;     Show all 12 Subjects
Abstract:
... Resident microbes of the human body, particularly the gut microbiota, provide essential functions for the host, and, therefore, have important roles in human health as well as mitigating disease. It is difficult to study the mechanisms by which the microbiota affect human health, especially at a systems-level, due to heterogeneity of human genomes, the complexity and heterogeneity of the gut micro ...
DOI:
10.1016/j.copbio.2017.01.008
PubMed:
28189107
PubMed Central:
PMC5544573

16. Potential Pitfalls in Estimating Viral Load Heritability

Author:
Leventhal, Gabriel E.; Bonhoeffer, Sebastian
Source:
Trends in microbiology 2016 v.24 no.9 pp. 687-698
ISSN:
0966-842X
Subject:
microbial genetics,   etc   ; Human immunodeficiency virus; heritability; models; patients; phylogeny; risk; viral load;     Show all 8 Subjects
Abstract:
... In HIV patients, the set-point viral load (SPVL) is the most widely used predictor of disease severity. Yet SPVL varies over several orders of magnitude between patients. The heritability of SPVL quantifies how much of the variation in SPVL is due to transmissible viral genetics. There is currently no clear consensus on the value of SPVL heritability, as multiple studies have reported apparently d ...
DOI:
10.1016/j.tim.2016.04.008

17. Cohesin Releases DNA through Asymmetric ATPase-Driven Ring Opening

Author:
Elbatsh, Ahmed M.O.; Haarhuis, Judith H.I.; Petela, Naomi; Chapard, Christophe; Fish, Alexander; Celie, Patrick H.; Stadnik, Magda; Ristic, Dejan; Wyman, Claire; Medema, René H.; Nasmyth, Kim; Rowland, Benjamin D.
Source:
Molecular cell 2016 v.61 no.4 pp. 575-588
ISSN:
1097-2765
Subject:
microbial genetics,   etc   ; DNA; acetylation; adenosinetriphosphatase; antagonists; chromatids; chromatin; cohesion; humans; interphase; mitosis; mutants; yeasts;     Show all 13 Subjects
Abstract:
... Cohesin stably holds together the sister chromatids from S phase until mitosis. To do so, cohesin must be protected against its cellular antagonist Wapl. Eco1 acetylates cohesin’s Smc3 subunit, which locks together the sister DNAs. We used yeast genetics to dissect how Wapl drives cohesin from chromatin and identified mutants of cohesin that are impaired in ATPase activity but remarkably confer ro ...
DOI:
10.1016/j.molcel.2016.01.025
PubMed:
26895426
PubMed Central:
PMC4769319

18. Insights into the chemical logic and enzymatic machinery of NRPS assembly lines

Author:
Walsh, Christopher T.
Source:
Natural product reports 2016 v.33 no.2 pp. 127-135
ISSN:
1460-4752
Subject:
microbial genetics,   etc   ; biosynthesis; enzymology; genomics; gramicidin; mass spectrometry; nonribosomal peptides; polyketides; tyrocidine;     Show all 9 Subjects
Abstract:
... Appreciation that some cyclic peptide antibiotics such as gramicidin S and tyrocidine were nonribosomally synthesized has been known for 50 years. The past two decades of research including advances in bacterial genetics, genomics, protein biochemistry and mass spectrometry have codified the principles of assembly line enzymology for hundreds of nonribosomal peptides and in parallel for thousands ...
DOI:
10.1039/c5np00035a

19. An efficient method for genetic certification of Bacillus subtilis strains, prospective producers of biopreparations

Author:
Terletsky, V. P.; Tyshchenko, V. I.; Novikova, I. I.; Boikova, I. V.; Tyulebaev, S. D.; Shakhtamirov, I. Ya.
Source:
Microbiology 2016 v.85 no.1 pp. 71-76
ISSN:
0026-2617
Subject:
microbial genetics,   etc   ; Bacillus subtilis; European Union; antibacterial properties; bacteria; certification; genotyping; restriction endonucleases; secondary metabolites; Russia;     Show all 10 Subjects
Abstract:
... Genetic certification of commercial strains of bacteria antagonistic to phytopathogenic microorganisms guarantees their unequivocal identification and confirmation of safety. In Russia, unlike EU countries, genetic certification of Bacillus subtilis strains is not used. Based on the previously proposed double digestion selective label (DDSL) fingerprinting, a method for genetic identification and ...
DOI:
10.1134/S0026261716010136

20. Terrestrial and marine Antarctic fungi extracts active against Xanthomonas citri subsp. citri

Author:
Vieira, G.; Purić, J.; Morão, L.G.; dos Santos, J.A.; Inforsato, F.J.; Sette, L.D.; Ferreira, H.; Sass, D.C.
Source:
Letters in applied microbiology 2018 v.67 no.1 pp. 64-71
ISSN:
0266-8254
Subject:
microbial genetic resources,   etc   ; Cadophora; Citrus; Penicillium; Pseudogymnoascus; antibacterial properties; cell death; cell viability; fungi; habitats; malt; marine sediments; microbial growth; molecular systematics; plant pathogens; secondary metabolites; soil; viability assays; Antarctic region; Antarctica;     Show all 20 Subjects
Abstract:
... This study aims to obtain secondary metabolites extracts from filamentous fungi isolated from soil and marine sediments from Antarctica and assess its potential antibacterial activity on Xanthomonas citri subsp. citri, the agent of citrus canker. Metabolites production was conducted in Malt 2% broth at 15°C for 20 days after which intracellular and extracellular extracts were obtained. The extract ...
DOI:
10.1111/lam.12890