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microbialload, etc ; Artemia franciscana; Siphoviridae; Vibrio parahaemolyticus; adsorption; bacteriophages; biological control; cell growth; food pathogens; food safety; gastroenteritis; genomics; multiple drug resistance; pH; seafoods; seawater; Show all 16 Subjects
Abstract:
... Vibrio parahaemolyticus is a major seafood-borne zoonotic pathogen causing gastroenteritis in humans. Although antibiotics are the most effective chemical agents for V. parahaemolyticus, their abuse has caused the emergence of multidrug-resistant strains, affecting seafood safety. Here, we have isolated and characterized the V. parahaemolyticus bacteriophage VPG01 as an eco-friendly sustainable al ...
microbialload, etc ; COVID-19 infection; Escherichia coli; World Health Organization; environment; gastrointestinal system; health hazards; hygiene; ingestion; microbiological quality; microbiological risk assessment; models; pandemic; probability; recreation; sanitation; surface water; wastewater treatment; water pollution; watersheds; South Africa; Show all 21 Subjects
Abstract:
... The World Health Organization reported that COVID-19 cases reached 611,421,786 globally by September 23, 2022. Six months after the first reported case, the disease had spread rapidly, reaching pandemic status, leading to numerous preventive measures to curb the spread, including a complete shutdown of many activities worldwide. Such restrictions affected services like waste management, resulting ...
... qPCR absolute quantification requires the assessment of PCR reaction efficiency. This is achieved by performing serial dilutions of gDNA. Herein, we demonstrate that when quantifying bacterial load in mixed samples, reaction efficiency should not be used as a calibration curve since gDNA isolation efficiency is neglected, significantly impacting quantification. ...
microbialload, etc ; DNA; Escherichia coli; acetates; glass; silica; thiocyanates; Show all 7 Subjects
Abstract:
... •To bypass the problem of viable but non-culturable bacteria that cannot be isolated by culturable methods would be to isolate DNA from bacterial cells concentrated from water samples used as a template for the polymerase chain reactions (PCR). DNA extraction protocol (Omar et al. 2010) was used as a foundation for extracting Escherichia coli DNA from water. The method combinations i.e., guanidium ...
microbialload, etc ; dormancy; food science; genetic similarity; physiological state; risk; Show all 6 Subjects
Abstract:
... Within genetically similar populations, subpopulations in different physiological states co-exist and may have enhanced survival capacity upon transition into a new environment. Under suboptimal conditions a small fraction of the total population may be sublethally injured or enter dormancy states, such as persistence and a VBNC state. Dormant cells remain viable and are able to evade detection, u ...
microbialload, etc ; Holstein; disinfectants; iodine; milk quality; nisin; plate count; spoilage; Show all 8 Subjects
Abstract:
... The use of teat dips is one of the most effective strategies to control mastitis by preventing new intramammary infections. Reducing bacterial load on teat skin helps control the spread of pathogens and spoilage and improves the quality of milk. The objective of this study was to evaluate the reduction of bacterial populations through the application of bacteriocin-based teat formulas. Teats of 12 ...
microbialload, etc ; antibiotic resistance; bacteriocins; biosynthesis; food industry; protein synthesis; spoilage; Show all 7 Subjects
Abstract:
... Emerging evidence shows the trend of using safe and natural preservatives like bacteriocins in food processing. The increasing demand by the food industry to extend keeping quality and prevent spoilage of various food products has appealed for new preservatives and new methods of conservation. Further, to discover the novel spectrum of antimicrobial compounds that could effectively battle food-bor ...
microbialload, etc ; air; color; decontamination; microbial contamination; nonthermal processing; shelf life; taste; Show all 8 Subjects
Abstract:
... The action of nonthermal atmospheric air plasma, producing the rich amount of reactive particles from surrounding air, on strawberries microbial decontamination was investigated. The plasma produced by the multipoint AC corona discharge fed by a Tesla coil and applied on strawberries leads to a substantial reduction of microbial load without influencing the sensory properties like taste and color. ...
microbialload, etc ; composite films; food chemistry; gluten; onions; plate count; tensile strength; yeasts; Show all 8 Subjects
Abstract:
... The study aimed to improve the properties of SA-CMC film by gluten (G) blends and bioactive compounds from onion waste extracts (OWEs) peel (OPE) and stalk (OSE). The applicability of film on the quality of peeled shallot onion during storage was also examined. Water barrier (0.62 g/msPa × 10⁻¹⁴) and tensile strength (11.50 MPa) of G/SA-CMC film improved more than SA-CMC film (1.55 g/msPa × 10⁻¹³ ...
microbialload, etc ; applied ecology; botanical composition; community structure; fungi; grasslands; leaves; pathogens; sheep; Show all 9 Subjects
Abstract:
... In grasslands, the interactions of foliar fungal diseases and their host plants are largely dependent on grazing by large herbivores. However, the relative importance of direct (i.e. pathogen removal) and indirect effects (i.e. via changes in plant community composition) of long‐term grazing on foliar fungal diseases remains largely unexplored, especially under varied grazing intensities. We condu ...
microbialload, etc ; affordability; antioxidants; bioavailability; cold; flavor; fruits; hunger; income; juices; microgreens; risk; Show all 12 Subjects
Abstract:
... This paper call attention to the functional role of Microgreen, UV‐C treatment (eco‐friendly), and cold press juices. An evidential report states the frequent consumption of veggies can lower the risk of chronic diseases and hidden hunger among the population. Recent findings reveal the higher potential of microgreens than matured plants. Processing of MG into juices may increase the bioavailabili ...
microbialload, etc ; Lactococcus lactis subsp. lactis; antioxidant activity; antioxidants; cheesemaking; cheeses; spoilage; spoilage fungi; Show all 8 Subjects
Abstract:
... Cheese is generally susceptible to contamination by fungal spoilage organisms. The application of essential oils (EOs) as preservatives in dairy products has been growing recently. Thus, they need to be tested for various interactions before being used in cheese production processes. The aim of this study was first to determine the antifungal and antioxidant effects of two EOs and Lactococcus lact ...
microbialload, etc ; bulk density; cohesion; firmness; ozonation; particle size; solubility; viscosity; whey; whey powder; Show all 10 Subjects
Abstract:
... The impact of ozone treatment (OT) for 0 (control) 30, 60, 90 and 120 min to whey concentrate (WC) on powder flow, physicochemical, functional, and microbiological characteristics of whey powders (WPs) was examined. OT decreased the cohesiveness of WC, whereas the firmness, consistency and index of viscosity were not affected. OT caused higher L∗ and b∗ values in WP and improved the solubility and ...
microbialload, etc ; Hermetia illucens; Listeria monocytogenes; Salmonella Typhimurium; feeds; food security; novel foods; sanitation; Show all 8 Subjects
Abstract:
... Ensuring food security is one of the main challenges facing the world over the next 30 years. There is, thus, an urgent need to significantly increase the supply of sustainable protein that can be transformed into animal feed. Proteins from insects offer a valuable alternative. This article presents the results of challenge tests conducted to investigate the dynamics of the microbial load of Salmo ...
microbialload, etc ; antibiotic resistance; antibiotics; environmental health; humans; liquid manure; livestock; pig manure; risk; Show all 9 Subjects
Abstract:
... AIMS: Antibiotic‐resistant bacteria affect human and animal health. Hence, their environmental spread represents a potential hazard for mankind. Livestock farming is suspected to be a key factor for spreading antibiotic resistance; consumers expect organic farming to imply less environmental health risk. This study aimed to assess the role of manure from organic and conventional farms for spreadin ...
Arista Nienaber; Mumin Ozturk; Robin Dolman; Renee Blaauw; Lizelle L. Zandberg; Simone van Rensburg; Melinda Britz; Frank E. A. Hayford; Frank Brombacher; Du Toit Loots; Cornelius M. Smuts; Suraj P. Parihar; Linda Malan
microbialload, etc ; cell structures; diet; inflammation; interleukin-17; lungs; omega-3 fatty acids; tuberculosis; Show all 8 Subjects
Abstract:
... Non-resolving inflammation is characteristic of tuberculosis (TB). Given their inflammation-resolving properties, n-3 long-chain PUFA (n-3 LCPUFA) may support TB treatment. This research aimed to investigate the effects of n-3 LCPUFA on clinical and inflammatory outcomes of Mycobacterium tuberculosis-infected C3HeB/FeJ mice with either normal or low n-3 PUFA status before infection. Using a two-by ...
microbialload, etc ; angiogenesis; diabetic foot; granulation tissue; immune response; keratinocytes; morbidity; quality of life; Show all 8 Subjects
Abstract:
... A wound is considered hard-to-heal when, despite the appropriate clinical analysis and intervention, the wound area reduces by less than a third at four weeks and complete healing fails to occur within 12 weeks. The most prevalent hard-to-heal wounds are associated with underlying metabolic diseases or vascular insufficiency and include arterial, venous, pressure and diabetic foot ulcers. Their co ...
microbialload, etc ; angiogenesis; diabetic foot; granulation tissue; immune response; keratinocytes; morbidity; quality of life; Show all 8 Subjects
Abstract:
... A wound is considered hard-to-heal when, despite the appropriate clinical analysis and intervention, the wound area reduces by less than a third at four weeks and complete healing fails to occur within 12 weeks. The most prevalent hard-to-heal wounds are associated with underlying metabolic diseases or vascular insufficiency and include arterial, venous, pressure and diabetic foot ulcers. Their co ...
microbialload, etc ; Galleria mellonella; death; genome; mice; minocycline; models; mutants; pneumonia; rifampicin; spontaneous mutation; therapeutics; Show all 12 Subjects
Abstract:
... :Elizabethkingia anophelis is inherently resistant to multiple antibiotics, except minocycline. This study aimed to determine the in-vitro and in-vivo efficacy of minocycline monotherapy and combination therapy against susceptible strains and the impact of reduced minocycline susceptibility. : Three clinical isolates and one laboratory-induced mutant with reduced minocycline susceptibility were in ...
microbialload, etc ; ecology; environmental DNA; freshwater; meta-analysis; mitochondria; pH; systematic review; temperature; ultraviolet radiation; Show all 10 Subjects
Abstract:
... Environmental DNA (eDNA) has been used in a variety of ecological studies and management applications. The rate at which eDNA decays has been widely studied but at present it is difficult to disentangle study‐specific effects from factors that universally affect eDNA degradation. To address this, a systematic review and meta‐analysis was conducted on aquatic eDNA studies. Analysis revealed eDNA de ...