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1. Application of hematoxylin reagent for sperm cell separation in sexual crime evidence

Author:
Kim, Joo-Young; Kim, Man Il; Lee, Hye Hyeon; Kim, Hye Lim; Lee, Eun-Jung; Lee, Yang-Han; Hwang, In-Kwan; Chun, Byung-Won; Kang, Pil-Won
Source:
Forensic science international 2020 v.307 pp. 110114
ISSN:
0379-0738
Subject:
microsatellite repeats,   etc   ; DNA; crime; forensic sciences; genotyping; spermatozoa; staining;     Show all 7 Subjects
Abstract:
... Seminal evidence obtained from a sexual crime scene provides clues for solving a case through forensic analysis. However, most evidence collected from sexual crime scenes is a mixture of sperm cells and vaginal discharge. Therefore, separating the sperm cells from the seminal evidence is very important. In this study, we developed a separation method for effectively separating sperm cells using di ...
DOI:
10.1016/j.forsciint.2019.110114

2. Genetic diversity in muskmelon based on SSR markers and morphological traits under well-watered and water-deficit condition

Author:
Ansari, Waquar Akhter; Atri, Neelam; Yang, Luming; Singh, Bijendra; Pandey, Sudhakar
Source:
Biocatalysis and agricultural biotechnology 2020 v.26 pp. 101630
ISSN:
1878-8181
Subject:
microsatellite repeats,   etc   ; alleles; branching; females; flowering; fruits; genetic variation; genotype; horticulture; loci; males; muskmelons; quantitative traits;     Show all 13 Subjects
Abstract:
... In the present study genetic diversity among 48 muskmelon accessions was analyzed employing various morphological traits under well-watered and water-deficit condition and SSR markers. Maximum values for horticultural traits were, 44 cm for fruit polar circumference, 33.2 cm for fruit equatorial circumference, 21 for number of fruits, 41.5 for days to first male flowering, 44 for days to 50% male ...
DOI:
10.1016/j.bcab.2020.101630

3. Species delimitation within Campomanesia (Myrtaceae) using morphology and amplification profiles of microsatellite markers

Author:
Luber, Jaquelini; Christ, Jheniffer Abeldt; Ferreira, Marcia Flores da Silva; Carrijo, Tatiana Tavares
Source:
Revista brasileira de botânica 2020 v.43 no.1 pp. 131-137
ISSN:
0100-8404
Subject:
microsatellite repeats,   etc   ; Campomanesia; DNA fingerprinting; cluster analysis; genetic markers; heat; plant identification; regression analysis; species identification;     Show all 9 Subjects
Abstract:
... Campomanesia is a well-circumscribed genus within Myrtaceae, but the boundaries among some of its species remain poorly understood. Microsatellite amplification profiles have proven useful DNA fingerprinting for plant species identification, but have never been applied to the taxonomy of this genus. Here we studied 12 species of Campomanesia, including a set of related and non-related species, as ...
DOI:
10.1007/s40415-020-00583-x

4. Screening of Polymorphic SSR Molecular Markers Between Resistant and Susceptible Parents for Localization of Brown Rust Resistance Gene

Author:
Shan, Hongli; Li, Wenfeng; Huang, Yingkun; Wang, Xiaoyan; Zhang, Rongyue; Li, Jie; Luo, Zhiming; Yin, Jiong; Cang, Xiaoyan
Source:
Sugar tech 2020 v.22 no.1 pp. 1-7
ISSN:
0972-1525
Subject:
microsatellite repeats,   etc   ; Puccinia melanocephala; breeding; chromosome mapping; genetic markers; leaf rust; parents; resistance genes; screening; sugarcane;     Show all 10 Subjects
Abstract:
... Sugarcane brown rust induced by Puccinia melanocephala is an important global disease. Exploring novel resistance genes and breeding varieties with durable resistance is the most economical and effective way of controlling brown rust. The establishment of complete and high-density molecular genetics maps is a key prerequisite for the discovery and location of sugarcane brown rust resistance genes. ...
DOI:
10.1007/s12355-019-00750-9

5. Characterization and application of fluidic properties of trinucleotide repeat sequences by wax-on-plastic microfluidics

Author:
Qamar, Ahmad Zaman; Asefifeyzabadi, Narges; Taki, Motahareh; Naphade, Swati; Ellerby, Lisa M.; Shamsi, Mohtashim Hassan
Source:
Journal of materials chemistry B 2020 v.8 no.4 pp. 743-751
ISSN:
2050-7518
Subject:
microsatellite repeats,   etc   ; chemistry; electrophoresis; genomics; humans; hydrodynamics; microfluidic technology; monitoring; neuromuscular disorders; spectroscopy;     Show all 10 Subjects
Abstract:
... Trinucleotide repeat (TNR) sequences introduce sequence-directed flexibility in the genomic makeup of all living species leading to unique non-canonical structure formation. In humans, the expansions of TNR sequences are responsible for almost 24 neurodegenerative and neuromuscular diseases because their unique structures disrupt cell functions. The biophysical studies of these sequences affect th ...
DOI:
10.1039/c9tb02208b

6. Characterization of repeat arrays in ultra‐long nanopore reads reveals frequent origin of satellite DNA from retrotransposon‐derived tandem repeats

Author:
Vondrak, Tihana; Ávila Robledillo, Laura; Novák, Petr; Koblížková, Andrea; Neumann, Pavel; Macas, Jiří
Source:
Theplant journal 2020 v.101 no.2 pp. 484-500
ISSN:
0960-7412
Subject:
microsatellite repeats,   etc   ; Lathyrus sativus; chromosomes; genome; legumes; models; nanopores; satellite DNA; tandem repeat sequences;     Show all 9 Subjects
Abstract:
... Amplification of monomer sequences into long contiguous arrays is the main feature distinguishing satellite DNA from other tandem repeats, yet it is also the main obstacle in its investigation because these arrays are in principle difficult to assemble. Here we explore an alternative, assembly‐free approach that utilizes ultra‐long Oxford Nanopore reads to infer the length distribution of satellit ...
DOI:
10.1111/tpj.14546
PubMed:
31559657
PubMed Central:
PMC7004042

7. Detecting sequence variants in clinically important protozoan parasites

Author:
Calarco, Larissa; Barratt, Joel; Ellis, John
Source:
International journal for parasitology 2020 v.50 no.1 pp. 1-18
ISSN:
0020-7519
Subject:
microsatellite repeats,   etc   ; Plasmodium; Protozoa; Toxoplasma; genes; mutation rate; parasites; pathogenicity; pathogens; phylogeny; pipelines; single nucleotide polymorphism;     Show all 12 Subjects
Abstract:
... Second and third generation sequencing methods are crucial for population genetic studies, and variant detection is a popular approach for exploiting this sequence data. While mini- and microsatellites are historically useful markers for studying important Protozoa such as Toxoplasma and Plasmodium spp., detecting non-repetitive variants such as those found in genes can be fundamental to investiga ...
DOI:
10.1016/j.ijpara.2019.10.004

8. Individual assignment and mixed-stock analysis of short mackerel (Rastrelliger brachysoma) in the Inner and Eastern Gulf of Thailand: Contrast migratory behavior among the fishery stocks

Author:
Kongseng, Sirithorn; Phoonsawat, Ratanavaree; Swatdipong, Akarapong
Source:
Fisheries research 2020 v.221 pp. 105372
ISSN:
0165-7836
Subject:
microsatellite repeats,   etc   ; Rastrelliger; aquacultural and fisheries equipment; fisheries management; mackerel; migratory behavior; overfishing; spawning; Thailand;     Show all 9 Subjects
Abstract:
... Short mackerel (Rastrelliger brachysoma) is a pelagic-neritic and the most economically important fish in Thailand with the value reached 190 M USD in 2013. The short mackerel is however classified as data deficient in IUCN Red List. In particular, short mackerel resource in the Gulf of Thailand is recently drastically decreased due to overfishing. In this study, we applied the individual assignme ...
DOI:
10.1016/j.fishres.2019.105372

9. Molecular karyotypes of loquat (Eriobotrya japonica) aneuploids can be detected by using SSR markers combined with quantitative PCR irrespective of heterozygosity

Author:
Wen, Guo; Dang, Jiangbo; Xie, Zhongyi; Wang, Jinying; Jiang, Pengfei; Guo, Qigao; Liang, Guolu
Source:
Plant methods 2020 v.16 no.1 pp. 22
ISSN:
1746-4811
Subject:
microsatellite repeats,   etc   ; Eriobotrya japonica; aneuploidy; chromosomes; heterozygosity; karyotyping; linkage groups; loquats; open pollination; phenotype; progeny; quantitative polymerase chain reaction; triploidy;     Show all 13 Subjects
Abstract:
... BACKGROUND: Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for detecting the molecular karyotypes of aneuploids in plants. RESULTS: Based on this imbalance in chromosome dosage, a new approach (referred to as ‘SSR-qPCR’) combining simple sequ ...
DOI:
10.1186/s13007-020-00568-7
PubMed:
32123538
PubMed Central:
PMC7041098

10. Variation in genetic diversity of tree sparrow (Passer montanus) population in long-term environmental heavy metal polluted areas

Author:
Yang, Wenzhi; Ding, Jian; Wang, Shengnan; Yang, Ying; Song, Gang; Zhang, Yingmei
Source:
Environmental pollution 2020 v.263 pp. 114396
ISSN:
0269-7491
Subject:
microsatellite repeats,   etc   ; Passer montanus; bedrock; cadmium; feathers; genetic variation; heavy metals; heterozygosity; lead; models; pollution; variance; wild birds;     Show all 13 Subjects
Abstract:
... Genetic diversity is the bedrock of evolution. The “Genetic Erosion” hypothesis posits that environmental pollution could cause reduced genetic diversity. To explore the effects of heavy metal pollution on genetic diversity in natural populations, we selected an area with more than sixty years of heavy metal contamination (Baiyin, BY) and a relatively unpolluted one (Liujiaxia, LJX), and tree spar ...
DOI:
10.1016/j.envpol.2020.114396

11. Application of high resolution melting combined with DNA-based markers for quantitative analysis of olive oil authenticity and adulteration

Author:
Chedid, Elsa; Rizou, Myrto; Kalaitzis, Panagiotis
Source:
Food chemistry: X 2020 v.6 pp. 100082
ISSN:
2590-1575
Subject:
microsatellite repeats,   etc   ; DNA; adulterated products; cultivars; melting; olive oil; product authenticity; quantitative analysis; single nucleotide polymorphism; traceability;     Show all 10 Subjects
Abstract:
... A plethora of biotechnological methodologies is used to authenticate quality olive oils. Among the DNA-based approaches, SNPs and SSRs combined with high resolution melting (HRM) provide certain advantages such as speed, simplicity and reliability. SNP-HRM and SSR-HRM were used for the authentication of monovarietal olive oils as well as the quantification of varietal composition in olive oil DNA ...
DOI:
10.1016/j.fochx.2020.100082
PubMed:
32154510
PubMed Central:
PMC7058896

12. Genetic versus demographic stock structure of rainbow smelt in a large fragmented lake

Author:
Euclide, Peter T.; Pientka, Bernie; Ellen Marsden, J.
Source:
Journal of Great Lakes research 2020 v.46 no.3 pp. 622-632
ISSN:
0380-1330
Subject:
microsatellite repeats,   etc   ; Osmerus mordax; basins; demography; fish; fisheries management; gene flow; genetic variation; genotype; lakes; population dynamics; population structure;     Show all 12 Subjects
Abstract:
... Identification of fish stocks plays an important role in fisheries management, but stock identification often depends on the techniques used and the management goals as much as on actual population structure. Historically, stocks were identified by place of capture, population demography and morphology, but genetic stock identification has become a standard approach. Here, we evaluate the stock st ...
DOI:
10.1016/j.jglr.2020.02.009

13. Molecular confirmation of candidate Hsp70 gene associated with heat tolerance in BC₃F₂ advanced backcross lines and their phenotypic resemblance with recurrent chilli Kulai

Author:
Usman, Magaji G.; Rafii, Mohd Y.; Yusuff, Oladosu; Martini, Mohammad Y.; Ismail, Mohd R.; Ridzuan, Raihana
Source:
Acta agriculturæ Scandinavica 2020 v.70 no.3 pp. 252-264
ISSN:
1651-1913
Subject:
microsatellite repeats,   etc   ; agronomic traits; backcrossing; flowering; fruit yield; fruits; genes; genotype; heat tolerance; parents; phenotype; plant height; temperature; Malaysia;     Show all 14 Subjects
Abstract:
... Domestic production of chilli in Malaysia is insufficient which accounted for 52% self-sufficiency level. Poor performance of local varieties under high temperatures above 42°C is one of the major factors affecting chilli production. This study was conducted with the objective of introgress heat-tolerant gene (Hsps) from AVPP0702 donor into Kulai using marker-assisted backcrossing. A total of 68 S ...
DOI:
10.1080/09064710.2019.1692899

14. Ascoma genotyping and mating type analyses of mycorrhizas and soil mycelia of Tuber borchii in a truffle orchard established by mycelial inoculated plants

Author:
Leonardi, Pamela; Murat, Claude; Puliga, Federico; Iotti, Mirco; Zambonelli, Alessandra
Source:
Environmental microbiology 2020 v.22 no.3 pp. 964-975
ISSN:
1462-2912
Subject:
microsatellite repeats,   etc   ; Tuber aestivum; Tuber melanosporum; ascomata; genotype; genotyping; hermaphroditism; mycelium; mycorrhizae; orchards; sexual behavior; shrubs; soil; trees; truffles;     Show all 15 Subjects
Abstract:
... Tuber borchii (the Bianchetto truffle) is a heterothallic Ascomycete living in symbiotic association with trees and shrubs. Maternal and paternal genotype dynamics have already been studied for the black truffles Tuber melanosporum and Tuber aestivum but not yet for T. borchii. In this study, we analysed maternal and paternal genotypes in the first truffle orchard realized with plants inoculated w ...
DOI:
10.1111/1462-2920.14777

15. Development of informative genic SSR markers for genetic diversity analysis of Picrorhiza kurroa

Author:
Singh, Pradeep; Sharma, Ram Kumar
Source:
Journal of plant biochemistry and biotechnology 2020 v.29 no.1 pp. 144-148
ISSN:
0971-7811
Subject:
microsatellite repeats,   etc   ; Geographical Locations; Picrorhiza kurrooa; alleles; endangered species; genetic rescue; genetic variation; habitats; hepatoprotective effect; heterozygosity; population structure;     Show all 11 Subjects
Abstract:
... Picrorhiza kurroa is a medicinal perennial herb of economic importance due to its hepatoprotective properties mainly accounted by picroside I and picroside II. To fulfill the current demand of the market indiscriminate collection from its natural habitat pose a great threat to this endangered species. To strategize the conservation of natural populations, a set of 20 highly informative novel genic ...
DOI:
10.1007/s13562-019-00515-1

16. Different contributions of birds and mammals to seed dispersal of a fleshy-fruited tree

Author:
Tsunamoto, Yoshihiro; Naoe, Shoji; Masaki, Takashi; Isagi, Yuji
Source:
Basic and applied ecology 2020 v.43 pp. 66-75
ISSN:
1439-1791
Subject:
microsatellite repeats,   etc   ; Cornus controversa; DNA barcoding; birds; feces; frugivores; genotyping; immigration; mammals; seed dispersal; seeds; smell; trees;     Show all 13 Subjects
Abstract:
... Birds and mammals are important seed dispersers of fleshy-fruited plants. Although their behaviors are different, they frequently consume the same species. Thus to understand the dispersal of fleshy-fruited plants, the contribution of birds and mammals to seed dispersal has to be evaluated. Besides, within birds or mammals, some species may functionally different from others. In this study, we exa ...
DOI:
10.1016/j.baae.2019.07.005

17. Molecular characterization of genetic diversity and similarity centers of safflower accessions with ISSR markers

Author:
Ali, Fawad; Nadeem, Muhammad Azhar; Habyarimana, Ephrem; Yılmaz, Abdurrahim; Nawaz, Muhammad Amjad; Khalil, Iftikhar Hussain; Ercişli, Sezai; Chung, Gyuhwa; Chaudhary, Hassan Javed; Baloch, Faheem Shehzad
Source:
Revista brasileira de botânica 2020 v.43 no.1 pp. 109-121
ISSN:
0100-8404
Subject:
microsatellite repeats,   etc   ; Carthamus tinctorius; alleles; arithmetics; genetic improvement; genetic variation; heterozygosity; multidimensional scaling; plant breeding; population structure; variance; Israel; Pakistan;     Show all 13 Subjects
Abstract:
... Crop genetic resources are vital inputs in crop genetic improvement. In this study, genetic diversity, population structure, and similarity centers for 131 safflower accessions obtained from 28 countries were investigated using 12 ISSR markers. A sum of 201 ISSR bands were obtained among which 188 (93.844%) were found polymorphic. Mean polymorphism information content (0.448) and diversity paramet ...
DOI:
10.1007/s40415-019-00574-7

18. Analysis of the genetic structure and morphology of Polygonatum cyrtonema in Anhui Province, eastern China revealed three distinct genetic groups

Author:
Ji, Rongrong; Sheng, Yajuan; Chen, Longsheng; Li, Xiaohong; Shao, Jianwen
Source:
Nordic journal of botany 2020 v.38 no.2
ISSN:
0107-055X
Subject:
microsatellite repeats,   etc   ; Polygonatum cyrtonema; breeding; cultivation area; genetic resources; genetic structure; genetic variation; geographical distribution; leaves; mountains; peduncle; China;     Show all 12 Subjects
Abstract:
... Polygonatum cyrtonema Hua, a rhizome‐propagating herb endemic to China, is used in many traditional Chinese medicines and foods. The hilly mountains in western and southern Anhui province is one of its main natural distribution and artificial cultivation areas. We assessed the genetic diversity and structure of P. cyrtonema germplasm resources in Anhui by nine pairs of SSR primers and selected mor ...
DOI:
10.1111/njb.02643

19. Genetic characterization of the entire range of Cycas panzhihuaensis (Cycadaceae)

Author:
Xiao, Siyue; Ji, Yunheng; Liu, Jian; Gong, Xun
Source:
Plant diversity 2020 v.42 no.1 pp. 7-18
ISSN:
2468-2659
Subject:
microsatellite repeats,   etc   ; Cycas; chloroplast DNA; conservation areas; endangered species; extinction; genes; genetic variation; haplotypes; phylogeography; population genetics; risk; watersheds; China;     Show all 14 Subjects
Abstract:
... Cycas panzhihuaensis L. Zhou & S. Y. Yang (Cycadaceae) is an endangered gymnosperm species endemic to the dry-hot valley of the Jinsha River basin in southwest China. Although the wild C. panzhihuaensis population from Panzhihua Cycad Natural Reserve is well protected and its genetic diversity has been well assessed, the genetic characteristics of populations outside the nature reserve, which face ...
DOI:
10.1016/j.pld.2019.10.001
PubMed:
32140633
PubMed Central:
PMC7046506

20. Genetic diversity of Nuphar lutea in Lithuanian river populations

Author:
Vyšniauskienė, Regina; Rančelienė, Vida; Naugžemys, Donatas; Rudaitytė-Lukošienė, Eglė; Patamsytė, Jolanta; Butkauskas, Dalius; Kupčinskienė, Eugenija; Žvingila, Donatas
Source:
Aquatic botany 2020 v.161 pp. 103173
ISSN:
0304-3770
Subject:
microsatellite repeats,   etc   ; Nuphar lutea; gene flow; genetic variation; heterozygosity; inbreeding; macrophytes; multidimensional scaling; rivers; subwatersheds; variance; Baltic States; Lithuania;     Show all 13 Subjects
Abstract:
... Currently, in Europe, increasing attention is being paid to the genetic diversity of aquatic macrophytes. Insufficient information exists about the river plants of the Baltic states. Our study aimed to evaluate the genetic diversity of Nuphar lutea individuals growing in Lithuanian watercourses. Eighteen populations were studied in the river catchments of Lithuania: Nemunas, Venta and Lielupė. The ...
DOI:
10.1016/j.aquabot.2019.103173