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microsatelliterepeats, etc ; RNA; dielectric spectroscopy; mutation; Show all 4 Subjects
Abstract:
... We present a PNA microprobe sensing platform to detect trinucleotide repeat mutation by electrochemical impedance spectroscopy. The microprobe platform discriminated Huntington's disease-associated CAG repeats in cell-derived total RNA with S/N 1 : 3. This sensitive, label-free, and PCR-free detection strategy may be employed in the future to develop biosensing platforms for the detection of a ple ...
microsatelliterepeats, etc ; DNA; Schizophyllum commune; genome; phylogeny; population genetics; Show all 6 Subjects
Abstract:
... Simple sequence repeats of DNA (SSRs) are the most popular source of genetic markers used in population genetics, phylogenetics, and genetic mapping. A large number of nucleotide repeats enriched in G and C were identified. 336 mononucleotide motifs with more than ten repeats were recorded. 2020 nucleotide repeats were identified, of which 97.4% are di- (68.2%) and trinucleotides (29.2%). The tota ...
microsatelliterepeats, etc ; Zephyranthes; allopatry; chemotaxonomy; lycorine; phenotypic plasticity; sympatry; Mexico; Show all 8 Subjects
Abstract:
... Zephyranthes (Amaryllidaceae) is a taxonomically complex genus due to the frequent overlap of interspecific morphological variation. In Mexico, Z. alba and Z. fosteri are herbaceous plants that, when distributed in sympatry, generate individuals with complex patterns of morphological variation, leading to taxonomic confusion. Therefore, it is necessary to first characterize these species in allopa ...
microsatelliterepeats, etc ; Prunus dulcis; almonds; flower color; phenotype; quantitative traits; Show all 6 Subjects
Abstract:
... Almond is one of the most important nut crops in Turkey. Simple sequence repeat (SSR)‐based linkage maps are very reliable in constructing linkage maps due to the co‐dominant and highly reproducible nature of the SSR markers. The objective of this study was to construct well‐saturated, SSR‐based genetic linkage maps using ‘Gulcan‐2 × Lauranne’ and ‘Guara × Nurlu’ F₁ populations and to identify QTL ...
microsatelliterepeats, etc ; DNA; allotetraploidy; centromeres; chromatin; genome; non-coding RNA; retrotransposons; Show all 8 Subjects
Abstract:
... Coffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and ...
microsatelliterepeats, etc ; Holstein; Jersey; alleles; ecotypes; genetic variation; genotyping; heterozygosity; loci; Show all 9 Subjects
Abstract:
... The application of information-entropy analysis on real data is shown: 84 bulls of Jersey (n = 10), Ayrshire (n = 10), red Danish (n = 10), red Swedish (n = 9) breeds and Holstein ecotypes of German (n = 13), Dutch (n = 17) and North American (n = 15) breeding genotyped by 11 microsatellite loci. The Shannon information index for the combined breed samples was 1.695, the weighted average for the s ...
microsatelliterepeats, etc ; apples; cultivars; evolution; fruits; genetic variation; germplasm; germplasm conservation; Show all 8 Subjects
Abstract:
... Simple sequence repeat (SSR) microsatellite markers have been extensively used to identify duplication and analyse genetic diversity in germplasm collections of apple. Here, we present findings from the use of a standard set of SSR loci in the managed repropagation of a significant international germplasm collection: the UK National Fruit Collection (NFC). A subset of eight SSR loci was deemed suf ...
Brian A. Tangen; Sheel Bansal; Joanna R. Freeland; Steven E. Travis; Jennifer D. Wasko; Terence P. McGonigle; L. Gordon Goldsborough; Keira Gow; Joy E. Marburger; Jacob A. Meier
microsatelliterepeats, etc ; administrative management; habitats; heterosis; wetlands; North America; Prairie Pothole region; Show all 7 Subjects
Abstract:
... The Prairie Pothole Region (PPR) of North America has experienced extreme changes in wetland habitat due to proliferation of invasive plants. Typha × glauca is a highly competitive hybrid between native T. latifolia and non-native T. angustifolia, and it is likely the predominant taxon in PPR wetlands. Genetics-based studies are limited, and distributions are poorly known for the first-generation ...
microsatelliterepeats, etc ; Mangifera indica; cluster analysis; genetic markers; genetic variation; germplasm; mangoes; Show all 7 Subjects
Abstract:
... Forty-eight mango accessions were compared for genetic diversity and relationships based on four molecular marker assays, namely, inter-simple sequence repeat (ISSR), sequence-related amplified polymorphism (SRAP), CAAT-box derived polymorphism (CBDP), and cis-element amplified polymorphism (CEAP). Comparative analyses of the marker assays based on Nei's genetic diversity (H), Shannon's informatio ...
microsatelliterepeats, etc ; allelic variation; genetic distance; genetic structure; genotyping; heterozygosity; loci; sheep; Show all 8 Subjects
Abstract:
... The aim of this study was to compare the genetic structure and genetic diversity of two Bulgarian sheep breeds. A total of 41 individuals from a modern Bulgarian sheep population (Rhodopean Tsigai, RT) and 46 representatives of a local Bulgarian sheep population (Middle Rhodopean Sheep, MRS) were investigated based on 12 STR (short tandem repeat, also known as microsatellite) markers. In total, 12 ...
microsatelliterepeats, etc ; DNA; Lagostrophus fasciatus; exposure duration; feces; genotyping errors; population size; Show all 7 Subjects
Abstract:
... Monitoring programs for populations of small or medium-sized animals often use live-capture or photo-monitoring trapping methods to estimate population size. The banded hare-wallaby (Lagostrophus fasciatus), a small macropodiform marsupial, does not readily enter traps or have individually unique distinguishing physical features and is consequently difficult to monitor using these methods. Isolati ...
microsatelliterepeats, etc ; Cervus; Lansium domesticum; common names; evolution; fruits; genetic markers; genetic variation; Show all 8 Subjects
Abstract:
... Morphological characters and SSR (Simple Sequence Repeat) molecular marker information were combined to study the genetic diversity of Lansium domesticum Corrêa. The research aimed to study the genetic diversity of accessions of L. parasiticum (duku, langsat, kokosan) accessions based on morphological characters and SSR markers. The field research was conducted at the Bogor Botanical Gardens, Meka ...
Michel Breuil; David Schikorski; Barbara Vuillaume; Ulrike Krauss; Jennifer C. Daltry; Glenroy Gaymes; Joanne Gaymes; Olivier Lepais; Nicolas Bech; Mišel Jelić; Thomas Becking; Frédéric Grandjean
microsatelliterepeats, etc ; genetic variation; introgression; population structure; purebreds; Caribbean; Saint Lucia; South America; Show all 8 Subjects
Abstract:
... AbstractThe newly described horned iguanaIguana insularis from the southern Lesser Antilles is separated in two easily recognized subspecies: I. insularis sanctaluciae from St. Lucia and I. insularis insularis from the Grenadines. Its former description is completed by the use of 38 new samples for genetic and morphological analysis. Seventeen microsatellites were used to estimate genetic diversi ...
microsatelliterepeats, etc ; Alligator mississippiensis; Crocodylus porosus; Gavialis gangeticus; comparative study; metagenomics; species identification; transcriptomics; Show all 8 Subjects
Abstract:
... Next-generation sequencing has allowed us to explore new methods, where comparative and population genomics can be used simultaneously. Keeping this in mind, we surveyed and analyzed the frequency and distribution of microsatellites in the Indian gharial (Gavialis gangeticus) and compared it with American alligator (Alligator mississippiensis) and saltwater crocodile (Crocodylus porosus) to enrich ...
microsatelliterepeats, etc ; DNA; algorithms; biotechnology; genetic variation; germplasm conservation; medicinal plants; multidimensional scaling; Iran; Show all 9 Subjects
Abstract:
... The analysis of genetic diversity in medicinal plant species can greatly facilitate germplasm conservation and selection for use in breeding schemes. The aim of the present study was to assess the genetic diversity and differentiation of eight Plantago species using inter simple sequence repeat (ISSR) markers. Thirty-one Plantago accessions belonging to eight species were collected from Iran envir ...
microsatelliterepeats, etc ; artificial insemination; dairy sheep; genetic variation; heterozygosity; population structure; sheep; Mediterranean region; Show all 8 Subjects
Abstract:
... This study analyzed the genetic variability, inbreeding and population structure of the Tunisian–North African dairy sheep breed, the Sicilo-Sarde (SS), created by crossing the Sarda and Comisana dairy breeds. The level of variability in the SS, considered as an endangered breed after a dramatic decrease, was assessed using 17 microsatellite markers by analyzing the two breed populations sampled f ...
microsatelliterepeats, etc ; Prunus armeniaca; apricots; evolution; genetic variation; germplasm; heterozygosity; population structure; variance; India; Show all 10 Subjects
Abstract:
... The collection and characterization of apricot germplasm is an important step for its utilization, conservation, and breeding programs. In the present study, the genetic diversity and population structure of 106 accessions of apricot including 82 indigenous and 24 exotic accessions collected from various geographical locations of Jammu and Kashmir, India were evaluated using 14 inter simple sequen ...
Kimberley G. Barrett; Geneviève Amaral; Melanie Elphinstone; Malcolm L. McAdie; Corey S. Davis; Jasmine K. Janes; John Carnio; Axel Moehrenschlager; Jamieson C. Gorrell
microsatelliterepeats, etc ; Marmota; conservation genetics; endangered species; extinction; genetic similarity; genetic variation; genotyping; risk; Show all 9 Subjects
Abstract:
... Captive breeding is often a last resort management option in the conservation of endangered species which can in turn lead to increased risk of inbreeding depression and loss of genetic diversity. Thus, recording breeding events via studbook for the purpose of estimating relatedness, and facilitating mating pair selection to minimize inbreeding, is common practice. However, as founder relatedness ...
microsatelliterepeats, etc ; DNA; amylose; databases; flowers; gelatinization temperature; odors; rice; stigma; Himalayan region; India; Show all 11 Subjects
Abstract:
... Kalonunia is a traditional black-husked non-Basmati type scented rice of terai region (foothills of eastern Himalayas) of West Bengal, India. With a view to develop the morpho-genetic database of the unique variety, the field test was conducted at Bidhan Chandra Krishi Viswavidyalaya (BCKV), Kalyani, West Bengal, India during kharif (wet) season of 2013, 2014 and 2018, and molecular characterizati ...
microsatelliterepeats, etc ; Siberia; allelic variation; genetic structure; heterozygosity; population genetics; population structure; sheep; steppes; Show all 9 Subjects
Abstract:
... Using 11 microsatellite markers, data on the genetic structure, levels of variability, and the degree of differentiation of 23 populations (1110 head) of Tuvan coarse-wooled short fat-tailed sheep from different regions of the Tyva Republic were obtained. It was established that at present this autochthonous breed is represented by two main types (mountain and steppe), as well as by numerous local ...