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microsatelliterepeats, etc ; Bayesian theory; Lutjanus argentimaculatus; aquaculture; cluster analysis; gene flow; genetic resources; genetic structure; genetic variation; marine fish; multidimensional scaling; Arabian Sea; Bay of Bengal; Show all 13 Subjects
Abstract:
... The mangrove red snapper, Lutjanus argentimaculatus, is a marine fish of key economic and cultural importance in the Indo-Pacific region. It is now considered much more of an important aquaculture species than capture fisheries. The present study aimed to reveal the genetic structure of this candidate species from the Arabian Sea and Bay of Bengal using microsatellite markers. Twelve microsatellit ...
microsatelliterepeats, etc ; RNA; dielectric spectroscopy; mutation; Show all 4 Subjects
Abstract:
... We present a PNA microprobe sensing platform to detect trinucleotide repeat mutation by electrochemical impedance spectroscopy. The microprobe platform discriminated Huntington's disease-associated CAG repeats in cell-derived total RNA with S/N 1 : 3. This sensitive, label-free, and PCR-free detection strategy may be employed in the future to develop biosensing platforms for the detection of a ple ...
microsatelliterepeats, etc ; Jatropha; apomixis; biofuels; progeny; Show all 5 Subjects
Abstract:
... In the present study, we aimed to estimate the upper limit of apomixis occurring in Jatropha under field conditions. We used unlinked microsatellite and AFLP markers on progeny arrays of six individuals which were designed to facilitate the detection of apomixis events more efficiently than previous reports. With 5-7 microsatellite markers, 5 of 90 progenies were identical to their maternal parent ...
microsatelliterepeats, etc ; DNA; neurodegenerative diseases; thermal stability; Show all 4 Subjects
Abstract:
... Minidumbbell (MDB) is a noncanonical DNA structure found to form in several pyrimidine‐rich short tandem repeats associated with neurodegenerative diseases. The most recently reported MDB contains two pentaloops formed by ATTCT repeats. Here, we studied the effects of a purine residue and a backbone discontinuous site on the structure and thermal stability of MDBs containing two pentaloops. It was ...
microsatelliterepeats, etc ; DNA; droplets; electrophoresis; forensic sciences; Show all 5 Subjects
Abstract:
... Because of its excellent monodispersity, high throughput, and low volume, microfluidics‐based droplet PCR has become the core technology of digital PCR, next‐generation sequencing, and other technology platforms. This study constructed a microfluidic water‐in‐oil droplet PCR system and amplified a commercially available forensic 22‐plex short tandem repeat detection system. We analyzed the sensiti ...
microsatelliterepeats, etc ; Potamotrygonidae; fish; freshwater; genotyping; paternity; Show all 6 Subjects
Abstract:
... Multiple paternity (MP) is a phenomenon observed for more than 30 elasmobranch species. The Batoidea is more specious than the Selachii, but only three studies of MP have been conducted on batoids. The occurrence of MP in freshwater stingrays was tested using microsatellite markers, which were developed for Potamotrygon leopoldi. Six mothers and their litters were genotyped, providing the first ev ...
microsatelliterepeats, etc ; DNA; DNA replication; forensic sciences; heterozygosity; Show all 5 Subjects
Abstract:
... Obtaining a full short tandem repeat (STR) profile from a low template DNA (LT-DNA) still presents a challenge for conventional methods due to significant stochastic effects and polymerase slippage. A novel amplification method with a lower cost and higher accuracy is required to improve the DNA amount. Previous studies suggested that DNA polymerases without bypass activity could not perform proce ...
microsatelliterepeats, etc ; DNA; Schizophyllum commune; genome; phylogeny; population genetics; Show all 6 Subjects
Abstract:
... Simple sequence repeats of DNA (SSRs) are the most popular source of genetic markers used in population genetics, phylogenetics, and genetic mapping. A large number of nucleotide repeats enriched in G and C were identified. 336 mononucleotide motifs with more than ten repeats were recorded. 2020 nucleotide repeats were identified, of which 97.4% are di- (68.2%) and trinucleotides (29.2%). The tota ...
microsatelliterepeats, etc ; Abies lasiocarpa; forests; heterozygosity; research; North America; Show all 6 Subjects
Abstract:
... Subalpine fir, Abies lasiocarpa, occurs throughout western North America, often in forest–tundra parkland. To resolve the presence of varieties in this species, we surveyed microsatellite genetic markers in 11 populations containing three putative varieties of Abies lasiocarpa: (1) var. lasiocarpa, (2) var. bifolia, and (3) var. arizonica. We tested primers from related taxa, and 13 of the best pr ...
microsatelliterepeats, etc ; Manglietia; Michelia; chloroplast genome; phylogeny; phytochemicals; trees; Show all 7 Subjects
Abstract:
... Magnoliaceae plants are industrial tree species with high ornamental and medicinal value. We published six complete chloroplast genomes of Magnoliaceae by using Illumina sequencing. These showed a typical quadripartite structure of angiosperm and were 159,901–160,008 bp in size. A total of 324 microsatellite loci and six variable intergenic regions (Pi > 0.01) were identified in six genomes. Compa ...
microsatelliterepeats, etc ; Chelydra serpentina; Macrochelys temminckii; alligators; threatened species; turtles; Show all 6 Subjects
Abstract:
... The alligator snapping turtle (Macrochelys temminckii) has been proposed as a Threatened species under the US Endangered Species Act and is considered Vulnerable by the International Union for the Conservation of Nature. To provide additional markers for conservation genetic studies, we identified 60 potential microsatellite loci, with 38 being polymorphic in M. temminckii, as well as successful c ...
microsatelliterepeats, etc ; genes; genomics; peptides; proteomics; ribosomes; sarcoma; transcription (genetics); Show all 8 Subjects
Abstract:
... Many cancers are characterized by gene fusions encoding oncogenic chimeric transcription factors (TFs) such as EWS::FLI1 in Ewing sarcoma (EwS). Here, we find that EWS::FLI1 induces the robust expression of a specific set of novel spliced and polyadenylated transcripts within otherwise transcriptionally silent regions of the genome. These neogenes (NGs) are virtually undetectable in large collecti ...
microsatelliterepeats, etc ; Prunus dulcis; almonds; flower color; phenotype; quantitative traits; Show all 6 Subjects
Abstract:
... Almond is one of the most important nut crops in Turkey. Simple sequence repeat (SSR)‐based linkage maps are very reliable in constructing linkage maps due to the co‐dominant and highly reproducible nature of the SSR markers. The objective of this study was to construct well‐saturated, SSR‐based genetic linkage maps using ‘Gulcan‐2 × Lauranne’ and ‘Guara × Nurlu’ F₁ populations and to identify QTL ...
microsatelliterepeats, etc ; Bayesian theory; Falco peregrinus; genetic structure; genetic variation; Show all 5 Subjects
Abstract:
... The main objective of this study was to determine the impact of increased demand for peregrine falcons via breeding (mainly Polish, Czech, German and Slovak) on the genetic structure of the birds. In the analysis, 374 specimens from six countries were sampled in 2008–2019 (omitting 2009), and all the birds analyzed were released into the wild as part of the Polish reintroduction program. The asses ...
microsatelliterepeats, etc ; Zephyranthes; allopatry; chemotaxonomy; lycorine; phenotypic plasticity; sympatry; Mexico; Show all 8 Subjects
Abstract:
... Zephyranthes (Amaryllidaceae) is a taxonomically complex genus due to the frequent overlap of interspecific morphological variation. In Mexico, Z. alba and Z. fosteri are herbaceous plants that, when distributed in sympatry, generate individuals with complex patterns of morphological variation, leading to taxonomic confusion. Therefore, it is necessary to first characterize these species in allopa ...
microsatelliterepeats, etc ; DNA; allotetraploidy; centromeres; chromatin; genome; non-coding RNA; retrotransposons; Show all 8 Subjects
Abstract:
... Coffea spp. chromosomes are very small and accumulate a variety of repetitive DNA families around the centromeres. However, the proximal regions of Coffea chromosomes remain poorly understood, especially regarding the nature and organisation of the sequences. Taking advantage of the genome sequences of C. arabica (2n = 44), C. canephora, and C. eugenioides (C. arabica progenitors with 2n = 22) and ...
microsatelliterepeats, etc ; Blattodea; Coptotermes formosanus; entomology; genetic relationships; lakes; mitochondria; California; Show all 8 Subjects
Abstract:
... New infestations of the Formosan subterranean termite, Coptotermes formosanus Shiraki (Blattodea: Rhinotermitidae), were discovered in southern California, namely in Rancho Santa Fe and La Mesa (San Diego County) and Highland Park (Los Angeles County) in 2021. We investigated whether these new infestations were related to the previous infestations in La Mesa (2018) and Canyon Lake, Riverside Count ...
microsatelliterepeats, etc ; Holstein; Jersey; alleles; ecotypes; genetic variation; genotyping; heterozygosity; loci; Show all 9 Subjects
Abstract:
... The application of information-entropy analysis on real data is shown: 84 bulls of Jersey (n = 10), Ayrshire (n = 10), red Danish (n = 10), red Swedish (n = 9) breeds and Holstein ecotypes of German (n = 13), Dutch (n = 17) and North American (n = 15) breeding genotyped by 11 microsatellite loci. The Shannon information index for the combined breed samples was 1.695, the weighted average for the s ...
microsatelliterepeats, etc ; Bayesian theory; Hypophthalmichthys molitrix; fish; genetic variation; heterozygosity; China; Show all 7 Subjects
Abstract:
... Hypophthalmichthys molitrix is one of the four most important fish in China and has high breeding potential. However, simple sequence repeat (SSR) markers developed on H. molitrix genome level for genetic diversity analysis are limited. In this study, the distribution characteristics of SSRs in the assembled H. molitrix genome were analyzed, and new markers were developed to preliminarily evaluate ...
microsatelliterepeats, etc ; apples; cultivars; evolution; fruits; genetic variation; germplasm; germplasm conservation; Show all 8 Subjects
Abstract:
... Simple sequence repeat (SSR) microsatellite markers have been extensively used to identify duplication and analyse genetic diversity in germplasm collections of apple. Here, we present findings from the use of a standard set of SSR loci in the managed repropagation of a significant international germplasm collection: the UK National Fruit Collection (NFC). A subset of eight SSR loci was deemed suf ...