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1. Tackling COVID-19 with neutralizing monoclonal antibodies

Author:
Davide Corti; Lisa A. Purcell; Gyorgy Snell; David Veesler
Source:
Cell 2021 v.184 no.12 pp. 3086-3108
ISSN:
0092-8674
Subject:
COVID-19 infection; autoimmunity; humans; pandemic; therapeutics
Abstract:
... Monoclonal antibodies (mAbs) have revolutionized the treatment of several human diseases, including cancer and autoimmunity and inflammatory conditions, and represent a new frontier for the treatment of infectious diseases. In the last 20 years, innovative methods have allowed the rapid isolation of mAbs from convalescent subjects, humanized mice, or libraries assembled in vitro and have proven ...
DOI:
10.1016/j.cell.2021.05.005

2. Reversibility and irreversibility in the temperature denaturation of monoclonal antibodies

Author:
Arne Schön; Ernesto Freire
Source:
Analytical biochemistry 2021 v.626 pp. 114240
ISSN:
0003-2697
Subject:
monoclonal antibodies,   etc   ; biochemistry; denaturation; heat; models; pH; temperature; therapeutics; viability;     Show all 9 Subjects
Abstract:
... There have been numerous studies of the temperature denaturation of monoclonal antibodies (mAbs) using differential scanning calorimetry (DSC). In general, mAbs are characterized by complex temperature denaturation transitions in which the various domains (CH2, CH3, Fab) give rise to different peaks in the heat capacity function. The complexity and overall irreversibility of the temperature ...
DOI:
10.1016/j.ab.2021.114240

3. Anti-CD20 monoclonal antibodies in Systemic Lupus Erythematosus

Author:
Kavina Shah; Mark Cragg; Maria Leandro; Venkat Reddy
Source:
Biologicals 2021 v.69 pp. 1-14
ISSN:
1045-1056
Subject:
monoclonal antibodies,   etc   ; B-lymphocytes; autoantibodies; lupus erythematosus; morbidity; mortality; nationalities and ethnic groups; pathogenesis; therapeutics; women;     Show all 10 Subjects
Abstract:
... between uncontrolled and controlled studies have raised doubts about its efficacy. In this review, we will focus on B cell abnormalities and the rationale behind B-cell depletion therapy with anti-CD20 monoclonal antibody (mAb), rituximab, will be explored including an evaluation of clinical and trial experience. Finally, we will discuss the mechanistic basis for considering alternative anti-CD20 mAbs. ...
DOI:
10.1016/j.biologicals.2020.11.002

4. Extremely potent human monoclonal antibodies from COVID-19 convalescent patients

Author:
Emanuele Andreano; Emanuele Nicastri; Ida Paciello; Piero Pileri; Noemi Manganaro; Giulia Piccini; Alessandro Manenti; Elisa Pantano; Anna Kabanova; Marco Troisi; Fabiola Vacca; Dario Cardamone; Concetta De Santi; Jonathan L. Torres; Gabriel Ozorowski; Linda Benincasa; Hyesun Jang; Cecilia Di Genova; Lorenzo Depau; Jlenia Brunetti; Chiara Agrati; Maria Rosaria Capobianchi; Concetta Castilletti; Arianna Emiliozzi; Massimiliano Fabbiani; Francesca Montagnani; Luisa Bracci; Giuseppe Sautto; Ted M. Ross; Emanuele Montomoli; Nigel Temperton; Andrew B. Ward; Claudia Sala; Giuseppe Ippolito; Rino Rappuoli
Source:
Cell 2021 v.184 no.7 pp. 1821-1835.e16
ISSN:
0092-8674
Subject:
monoclonal antibodies,   etc   ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; animal models; half life; humans; memory; pandemic; risk reduction; therapeutics; viruses;     Show all 11 Subjects
Abstract:
... Human monoclonal antibodies are safe, preventive, and therapeutic tools that can be rapidly developed to help restore the massive health and economic disruption caused by the coronavirus disease 2019 (COVID-19) pandemic. By single-cell sorting 4,277 SARS-CoV-2 spike protein-specific memory B cells from 14 COVID-19 survivors, 453 neutralizing antibodies were identified. The most potent ...
DOI:
10.1016/j.cell.2021.02.035

5. Broad neutralization of CSFV with novel monoclonal antibodies in vivo

Author:
Huiling Xu; Guangwei Han; Ying Lu; Zehui Liu; Lina Tao; Fang He
Source:
International journal of biological macromolecules 2021 v.173 pp. 513-523
ISSN:
0141-8130
Subject:
Classical swine fever virus; dose response; epitopes; glycoproteins; hog cholera; immunity; neutralization; polypeptides; streams; vaccination; vaccines; viruses; China
Abstract:
... , a batch of neutralizing monoclonal antibodies (mAbs) against E2, as alternative antiviral strategies, were produced. Among them, mAbs 6D10, 8D8 and 3C12 presented neutralizing reactivity against CSFV in a dose-dependent manner. Based on truncated overlapping fragments of E2 and mutants, three linear neutralizing epitopes were identified highly conserved in various CSFV strains. Epitopes ⁸YRYAIS ...
DOI:
10.1016/j.ijbiomac.2021.01.142

6. Discrimination between minimally modified LDL and fully oxidized LDL using monoclonal antibodies

Author:
Kanokwan Lowhalidanon; Panida Khunkaewla
Source:
Analytical biochemistry 2021 v.619 pp. 114103
ISSN:
0003-2697
Subject:
monoclonal antibodies,   etc   ; apolipoprotein B-100; assays; humans; lipid peroxidation; low density lipoprotein; oxidation; thiobarbituric acid-reactive substances;     Show all 8 Subjects
Abstract:
... of oxidized LDL, however this method is unable to distinguish between mm-LDL and oxLDL. In this study, seven specific monoclonal antibodies (mAbs) against human LDL were generated and selectively bound to the apolipoprotein B-100 (apoB-100) component of LDL. Oxidized LDL was produced by incubation of human LDL with 10 μM CuSO₄ for various times. The TBARS assay revealed that the optimal incubation time ...
DOI:
10.1016/j.ab.2021.114103

7. Novel conformation‐selective monoclonal antibodies against apoA‐I amyloid fibrils

Author:
Takashi Ohgita; Yuki Furutani; Miyu Nakano; Megumi Hattori; Ayane Suzuki; Miho Nakagawa; Sera Naniwa; Izumi Morita; Hiroyuki Oyama; Kazuchika Nishitsuji; Norihiro Kobayashi; Hiroyuki Saito
Source:
FEBS journal 2021 v.288 no.5 pp. 1496-1513
ISSN:
1742-464X
Subject:
amyloid; amyloidosis; apolipoprotein A-I; dissociation; hemocyanin; high density lipoprotein; humans; hybridomas; mutation; proline; sequence analysis; Iowa
Abstract:
... The Iowa (G26R) mutation in human apolipoprotein A‐I (apoA‐I), the major protein of plasma high‐density lipoprotein, is associated with systemic amyloidosis, and the N‐terminal 1–83 fragment of apoA‐I carrying this mutation has a strong propensity to form amyloid fibrils. Here, we generated and characterized novel monoclonal antibodies (mAbs) that display selective reactivity to apoA‐I amyloid ...
DOI:
10.1111/febs.15487

8. Pooling of monoclonal antibodies for the rapid detection of commercially important finfish

Author:
Yaozhou Zhu; Yun-Hwa Peggy Hsieh
Source:
Food control 2021 v.121 pp. 107581
ISSN:
0956-7135
Subject:
monoclonal antibodies,   etc   ; Lutjanus; Thunnus albacares; Xiphias gladius; allergenicity; analytical methods; cost effectiveness; cross reaction; enzyme-linked immunosorbent assay; fish; food allergens; food allergies; food animals; food law; food safety; foods; human nutrition; markets; polyunsaturated fatty acids; proteins; rapid methods; reproducibility; sampling; vitamins;     Show all 24 Subjects
Abstract:
... immunoassays have been developed to detect many food allergens, there is no effective immunoassay capable of recognizing all common food fish species on the market. The selectivities of three monoclonal antibodies (mAbs), 8F5, 2G9 and 2F3, known to exhibit anti-finfish activity against cooked crude fish protein extract from red snapper, yellow fin tuna and swordfish, respectively, were each tested for 63 ...
DOI:
10.1016/j.foodcont.2020.107581

9. Identification of monoclonal antibodies suitable for blocking IGF-1 receptors in the horse

Author:
S. Rahnama; N. Vathsangam; R. Spence; S.T. Anderson; M.A. de Laat; S. Bailey; M.N. Sillence
Source:
Domestic animal endocrinology 2021 v.74 pp. 106510
ISSN:
0739-7240
Subject:
monoclonal antibodies,   etc   ; analgesia; flow cytometry; horses; humans; hyperinsulinemia; insulin; insulin-like growth factor I receptor; laminitis; liver; prototypes;     Show all 11 Subjects
Abstract:
... and become downregulated after prolonged insulin infusion. As a first step toward confirming this mechanism and beginning to develop a therapeutic anti–IGF-1R monoclonal antibody (mAb) for horses, it was necessary to identify available human IGF-1R mAbs that would recognize equine receptors. Four IGF-1R mAbs were tested using soluble equine IGF-1R, with ELISA and flow cytometry. Frozen equine lamellar ...
DOI:
10.1016/j.domaniend.2020.106510

10. Development of monoclonal antibodies against recombinant LipL21 protein of pathogenic Leptospira through phage display technology

Author:
Mohammad Ridhuan Mohd Ali; Jia Siang Sum; Nurul Najian Aminuddin Baki; Yee Siew Choong; Nur Asyura Nor Amdan; Fairuz Amran; Theam Soon Lim
Source:
International journal of biological macromolecules 2021 v.168 pp. 289-300
ISSN:
0141-8130
Subject:
monoclonal antibodies,   etc   ; Leptospira; bacteriophages; computer simulation; early diagnosis; epitopes; germ cells; humans; immunogenicity; leptospirosis; sequence analysis; zoonoses;     Show all 12 Subjects
Abstract:
... Leptospirosis is a potentially fatal zoonosis that is caused by spirochete Leptospira. The signs and symptoms of leptospirosis are usually varied, allowing it to be mistaken for other causes of acute febrile syndromes. Thus, early diagnosis and identification of a specific agent in clinical samples is crucial for effective treatment. This study was aimed to develop specific monoclonal antibodies ...
DOI:
10.1016/j.ijbiomac.2020.12.062

11. Preferential light-chain labeling of native monoclonal antibodies improves the properties of fluorophore conjugates

Author:
Michael P. Luciano; Ivan Dingle; Saghar Nourian; Martin J. Schnermann
Source:
Tetrahedron letters 2021 v.75 pp. 153211
ISSN:
0040-4039
Subject:
monoclonal antibodies,   etc   ; esters; fluorescent dyes; humans;     Show all 4 Subjects
Abstract:
... Site specific labeling methods have significant potential to enhance the properties of antibody conjugates. While studied extensively in the context of antibody-drug conjugates (ADCs), few studies have examined the impact of homogenous labeling on the properties of antibody-fluorophore conjugates (AFCs). We report the application of pentafluorophenyl (PFP) esters, which had previously been shown t ...
DOI:
10.1016/j.tetlet.2021.153211

12. Middle-up characterization of monoclonal antibodies by online reduction liquid chromatography-mass spectrometry

Author:
Liesa Verscheure; Marie Oosterlynck; An Cerdobbel; Pat Sandra; Frederic Lynen; Koen Sandra
Source:
Journal of chromatography 2021 v.1637 pp. 461808
ISSN:
0021-9673
Subject:
asparagine; aspartic acid; corrosion; deamidation; denaturation; dithiothreitol; guanidines; immunoglobulin G; isomerization; liquid chromatography; mass spectrometry; stainless steel; therapeutics
Abstract:
... This study describes the fully automated middle-up characterization of monoclonal antibodies (mAbs) and next-generation variants by online reduction liquid chromatography-mass spectrometry (LC-MS). Proteins were trapped on-column and subjected to online desalting, denaturation and reduction prior to reversed phase elution of the created subunits in the MS. The evaluation of more than 20 different ...
DOI:
10.1016/j.chroma.2020.461808

13. Characterization of Anti-p54 Monoclonal Antibodies and Their Potential Use for African Swine Fever Virus Diagnosis

Author:
Weldu Tesfagaber; Lulu Wang; Ghebremedhin Tsegay; Yibrah Tekle Hagoss; Zhenjiang Zhang; Jiwen Zhang; Haoyue Huangfu; Fei Xi; Fang Li; Encheng Sun; Zhigao Bu; Dongming Zhao
Source:
Pathogens 2021 v.10 no.2 pp. -
ISSN:
2076-0817
Subject:
monoclonal antibodies,   etc   ; African swine fever; African swine fever virus; Escherichia coli; antibody detection; blood serum; confidence interval; enzyme-linked immunosorbent assay; serodiagnosis; swine; vaccines; viruses;     Show all 12 Subjects
Abstract:
... techniques are useful for a range of serological investigations. The aim of this study was to produce and characterize p54 monoclonal antibodies with an ultimate goal of developing a monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) for ASFV antibody detection. Five monoclonal antibodies against p54 protein expressed in Escherichia coli was generated and their characterizations were ...
DOI:
10.3390/pathogens10020178

14. Immunoinformatic epitope prediction to select monoclonal antibodies for Phl p 1 quantification

Author:
Azahara González-Ruiz; M. Ángeles López-Matas; Raquel Moya; Jerónimo Carnés
Source:
Molecular immunology 2021 v.136 pp. 1-7
ISSN:
0161-5890
Subject:
B-lymphocytes; Phleum pratense; allergens; chromatography; computer simulation; epitopes; freeze drying; immunoinformatics; immunotherapy; peptide libraries; prediction
Abstract:
... Allergen quantification has become a relevant parameter for allergen extract characterization and to guarantee the consistency of the manufacturing process at allergen immunotherapy. The aim of this study was to develop and validate a method to quantify the major allergen Phl p 1 based on a prediction of the antigenic regions by immunoinformatic strategies.Phl p 1 was purified from a Phleum praten ...
DOI:
10.1016/j.molimm.2021.05.007

15. Fast in‐line bottom‐up analysis of monoclonal antibodies: Toward an electrophoretic fingerprinting approach

Author:
Meriem Dadouch; Yoann Ladner; Claudia Bich; Jérôme Montels; Jacques Morel; Catherine Perrin
Source:
Electrophoresis 2021 v.42 no.11 pp. 1229-1237
ISSN:
0173-0835
Subject:
autolysis; capillary zone electrophoresis; denaturation; digestion; dithiothreitol; laminar flow; peptides; quality control; surfactants; temperature; trypsin
Abstract:
... For their characterization and quality control, monoclonal antibodies are frequently analyzed at the bottom‐up level to generate specific fingerprints that can be used to tackle post‐translational modifications or ensure production consistency between lots. To circumvent time‐consuming and labor‐intensive off‐line sample preparation steps, the implementation of integrated methodologies from ...
DOI:
10.1002/elps.202000375

16. Development of a competitive inhibition kinetic ELISA to determine the inhibition constant (Ki) of monoclonal antibodies

Author:
Alfredo Toraño; Inmaculada Moreno; José Antonio Infantes; Mercedes Domínguez
Source:
Journal of immunological methods 2021 v.493 pp. 113042
ISSN:
0022-1759
Subject:
monoclonal antibodies,   etc   ; antigens; colorimetry; enzymes; ligands; surface plasmon resonance;     Show all 6 Subjects
Abstract:
... Antibody-antigen interactions are mediated by the same molecular recognition mechanisms as those of an enzyme and its substrate. On this basis, we developed a competitive inhibition kinetic ELISA to measure monoclonal antibody (mAb) inhibition constants. Serially diluted samples of ligand (mAb) and inhibitor (soluble antigen) were incubated to equilibrium in ELISA plates coated with a fixed ...
DOI:
10.1016/j.jim.2021.113042

17. Research Note: Characterization of monoclonal antibodies and development of sandwich ELISA for detecting chicken IL7

Author:
Alfredo Panebra; Woo H. Kim; Yeong H. Hong; Hyun S. Lillehoj
Source:
Poultry science 2021 v.100 no.3 pp. 100940
ISSN:
0032-5791
Subject:
Western blotting; chickens; enzyme-linked immunosorbent assay; immunization; interleukin-7; neutralization; parasites; thymocytes
Abstract:
... IL7 is a hematopoietic growth factor required for development and maintenance of lymphocytes including T cells, B cells, and natural killer cells. Recently, chicken IL7 (chIL7) has been cloned and studied in viral and parasite infection models. However, no monoclonal antibodies (mAb) that specifically detect chIL7 have been developed so far. In this study, recombinant chIL7 that expressed ...
Handle:
10113/7231341
DOI:
10.1016/j.psj.2020.12.037
CHORUS:
10.1016/j.psj.2020.12.037

18. Development of Monoclonal Antibodies against Pea Globulins for Multiplex Assays Targeting Legume Proteins

Author:
Norbert Lidzba; Verónica García Arteaga; Andreas Schiermeyer; Heide Havenith; Isabel Muranyi; Stefan Schillberg; Jörg Lehmann; Elke Ueberham
Source:
Journal of agricultural and food chemistry 2021 v.69 no.9 pp. 2864-2874
ISSN:
1520-5118
Subject:
Lupinus; allergenicity; cross reaction; food chemistry; food safety; globulins; hypersensitivity; peanuts; peas; people; product safety; soybeans
Abstract:
... Legume proteins are widely used as food ingredients, but only some (soybean, lupin, and peanut) must be declared under consumer safety regulations to protect allergy sufferers. It is not yet mandatory to declare pea proteins as allergens even though they are predicted to be allergenic based on cross-reactivity in sensitized people. The processing of legume proteins can modify their allergenic prop ...
DOI:
10.1021/acs.jafc.0c07177

19. Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

Author:
Fengli Liu; Yanxin Cao; Maokai Yan; Mengxu Sun; Qingshui Zhang; Jun Wang; Guanghua Fu; Rongchang Liu; Yu Huang; Jingliang Su
Source:
Pathogens 2021 v.10 no.3 pp. -
ISSN:
2076-0817
Subject:
Anatid alphaherpesvirus 1; cell culture; cloaca; cross reaction; detection limit; diagnostic techniques; epizootic diseases; glycoproteins; immunoaffinity chromatography; viral enteritis; viruses; waterfowl; China
Abstract:
... Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically ...
DOI:
10.3390/pathogens10030365

20. Development of novel monoclonal antibodies and immunoassays for sensitive and specific detection of SULF1 endosulfatase

Author:
Yi-Wei Yang; Joanna J. Phillips; David M. Jablons; Hassan Lemjabbar-Alaoui
Source:
Biochimica et biophysica acta 2021 v.1865 no.2 pp. 129802
ISSN:
0304-4165
Subject:
Western blotting; biomarkers; blood; carcinogenesis; heparan sulfate; humans; immunohistochemistry; ligands; proteoglycans
Abstract:
... from HSPGs and thus regulates signaling mediated by 6-O sulfation on HSPGs. The expression of Sulf1 is altered in many cancers. Further studies are needed to clarify Sulf1 role in tumorigenesis, and new tools that can expand our knowledge in this field are required.We have developed and validated novel SULF1 monoclonal antibodies (mAbs). The isotype and subclass for each of these antibodies were ...
DOI:
10.1016/j.bbagen.2020.129802