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monoclonalantibodies, etc ; antiparasitic agents; detection limit; enzyme-linked immunosorbent assay; food composition; haptens; immunomagnetic separation; milk; Show all 8 Subjects
Abstract:
... Monepantel (MNP) is a new class of aminoacetonitrile derivative antiparasitic drug and mainly metabolizes as monepantel sulfone in vivo. This study aimed at developing a novel monepantel sulfone (MNPS) detection method combining indirect competitive enzyme-linked immunosorbent assay (icELISA) and immunomagnetic separation. An easy-synthesized hapten was designed based on the partial structure of M ...
... To determine gentamicin residues in animal tissues, a monoclonal antibody (Mab) was produced and a sensitive indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) was developed. At first, gentamicin was conjugated with bovine serum albumin as immunogens which were used to immunize BALB/c mice. Then, an anti-gentamicin Mab was prepared by hybridoma technology. Finally, a sensitive icCL ...
... Improving detection sensitivity is still a major research emphasis for lateral flow immunoassay (LFIA). Increasing the binding efficiency and stability of the probe is an achievable and effective solution. In this work, we developed a highly sensitive lateral flow immunoassay for clenbuterol detection by using bismuth sulfide nanoparticle (Bi₂S₃) nanoparticles (NPs) as a novel marker. Here, Bi₂S₃ ...
monoclonalantibodies, etc ; COVID-19 infection; Nicotiana benthamiana; Severe acute respiratory syndrome coronavirus 2; antigens; cellulose; cost effectiveness; detection limit; electrochemistry; electrodes; graphene; immunosensors; nanocrystals; saliva; voltammetry; Show all 15 Subjects
Abstract:
... The current approaches of diagnostic platforms for detecting SARS-CoV-2 infections mostly relied on adapting the existing technology. In this work, a simple and low-cost electrochemical sensing platform for detecting SAR-CoV-2 antigen was established. The proposed sensor combined the innovative disposable paper-based immunosensor and cost-effective plant-based anti-SARS-CoV-2 monoclonal antibody C ...
monoclonalantibodies, etc ; Salmonella Typhimurium; chickens; detection limit; electrodes; food safety; gluconic acid; glucose; glucose oxidase; hydrogen peroxide; magnetic fields; magnetic separation; mobile telephones; organ-on-a-chip; polyclonal antibodies; polystyrenes; Show all 16 Subjects
Abstract:
... Screening of pathogenic bacteria is an essential measure for ensuring food safety. A fast, sensitive and automatic platform for Salmonella determination was presented employing continuous-flow magnetic isolation, enzymatic impedance amplification and smartphone data analysis. First, magnetic nanoparticles coupling with polyclonal antibody, polystyrene spheres coupling with monoclonal antibody and ...
monoclonalantibodies, etc ; antigens; phosphates; viscosity; Show all 4 Subjects
Abstract:
... High-concentration and low-viscosity antibody formulations are necessary when administering these solutions subcutaneously (SC) due to limitations on injection volume. Here we show a method to decrease the viscosity of monoclonal antibody solution by protein–polyelectrolyte complex (PPC) with poly-l-glutamic acid (polyE). The viscosity of omalizumab solutions was 90 cP at the concentration of 150 ...
monoclonalantibodies, etc ; cytotoxicity; ligases; proteolysis; Show all 4 Subjects
Abstract:
... Degrader-antibody conjugates (DACs) are novel entities that combine a proteolysis targeting chimera (PROTAC) payload with a monoclonal antibody via some type of chemical linker. This review provides a current summary of the DAC field. Many general aspects associated with the creation and biological performance of traditional cytotoxic antibody-drug conjugates (ADCs) are initially presented. These ...
monoclonalantibodies, etc ; biopharmaceuticals; chromatography; fractionation; separation; Show all 5 Subjects
Abstract:
... This paper discusses ultrahigh‐speed, ultrahigh‐resolution preparative protein separation using an in‐house designed membrane chromatography device. The performance of the membrane chromatography device was systematically compared with an equivalent resin‐packed preparative column. Experiments carried out using model proteins showed that membrane chromatography gave more than four times greater re ...
monoclonalantibodies, etc ; cell culture; chromatography; hydroxyapatite; mammals; Show all 5 Subjects
Abstract:
... We describe and discuss a simple dry-compression technique for preparing a flat cuboid chromatography device containing a shallow packed-bed of crystalline hydroxyapatite nanoparticles. We then discuss the use of this device for fast protein separation in the bind-and-elute mode. Such separation could be carried out at quite low pressures, making it possible to use inexpensive low pressure chromat ...
monoclonalantibodies, etc ; filtrates; filtration; osmotic pressure; viscosity; Show all 5 Subjects
Abstract:
... A number of Single Pass Tangential Flow Filtration (SPTFF) systems have recently been commercialized for inline concentration of monoclonal antibodies and other biotherapeutics, both to resolve bottlenecks in existing processes and as part of the development of integrated continuous downstream processes. The objective of this study was to examine the design and optimization of SPTFF modules using ...
monoclonalantibodies, etc ; glycoproteins; humans; vaccine development; viruses; Show all 5 Subjects
Abstract:
... The filoviruses, including ebolaviruses and marburgviruses, are among the world’s deadliest pathogens. As the only surface-exposed protein on mature virions, their glycoprotein GP is the focus of current therapeutic monoclonal antibody discovery efforts. With recent technological developments, potent antibodies have been identified from immunized animals and human survivors of virus infections and ...
monoclonalantibodies, etc ; biopharmaceuticals; biotechnology; chemistry; subcutaneous injection; viscosity; Show all 6 Subjects
Abstract:
... For the development of concentrated monoclonal antibody formulations for subcutaneous administration, the main challenge is the high viscosity of the solutions. To compensate for this, viscosity reducing agents are commonly used as excipients. Here, we applied two computational chemistry approaches to discover new viscosity-reducing agents: fingerprint similarity searching, and physicochemical pro ...
monoclonalantibodies, etc ; detection limit; etoxazole; grapes; immunoaffinity chromatography; Show all 5 Subjects
Abstract:
... Etoxazole, a novel organofluorine pesticide, has been widely used against various mites in fruits and vegetables. In this study, we designed an etoxazole-hapten and produced a highly sensitive monoclonal antibody (mAb) against etoxazole with a half-maximal inhibitory concentration of 4.04 ng/mL. Based on the mAb, we developed an immunochromatographic assay (ICA) for the detection of etoxazole in o ...
monoclonalantibodies, etc ; cation exchange chromatography; lysozyme; mechanistic models; Show all 4 Subjects
Abstract:
... In preparative and industrial chromatography, the current viewpoint is that the dynamic binding capacity governs the process economy, and increased dynamic binding capacity and column utilization are achieved at the expense of productivity. The dynamic binding capacity in chromatography increases with residence time until it reaches a plateau, whereas productivity has an optimum. Therefore, the lo ...
monoclonalantibodies, etc ; beverages; diclofenac; immunoaffinity chromatography; quantitative analysis; tea; Show all 6 Subjects
Abstract:
... Diclofenac (DIC) is often mixed into health products by unscrupulous merchants to claim antirheumatic effects. In this study, A highly sensitive group-selective monoclonal antibody (mAb) was prepared, and an immunochromatographic assay was developed for rapid screening of DIC, diclofenac methyl (DICA) and N-phenylanthranilic acid (PAA) in health teas and health beverages. In our quantitative analy ...
monoclonalantibodies, etc ; equations; ligands; quantitative analysis; surface plasmon resonance; toxins; Show all 6 Subjects
Abstract:
... We describe an ELISA method with which to determine monoclonal antibody (mAb) on-rate constants (k₊₁) based on time-course data of ligand (L) binding to plate-bound mAb. The assay was performed in pseudo-first order kinetic conditions ([L] > > [mAb]) and at various starting ligand concentrations. Time-course initial velocity was analyzed by several methods to derive the pseudo-first order (kₒbₛ) a ...
monoclonalantibodies, etc ; cation exchange chromatography; hydrophobicity; molecular weight; product quality; Show all 5 Subjects
Abstract:
... The development of manufacturing processes for antibody-drug conjugates (ADCs) presents many challenges compared to a standard monoclonal antibody. Conjugation processes typically start with an antibody intermediate that was purified to have very low levels of aggregates. The additional processing required for ADCs, including a hydrophobic small molecule and co-solvents, contributes to unacceptabl ...
monoclonalantibodies, etc ; cancer therapy; cytokines; cytotoxicity; half life; lymphoma; macrophages; Show all 7 Subjects
Abstract:
... Monoclonal antibody (mAb) therapy has successfully been introduced as treatment of several lymphomas and leukemias. However, solid tumors reduce the efficacy of mAb therapy because of an immune-suppressive tumor micro-environment (TME), which hampers activation of effector immune cells. Pro-inflammatory cytokine therapy may counteract immune suppression in the TME and increase mAb efficacy, but un ...
monoclonalantibodies, etc ; chromatography; electrophoresis; glycopeptides; glycosylation; oligosaccharides; polysaccharides; spectroscopy; therapeutics; Show all 9 Subjects
Abstract:
... N-glycosylation of therapeutic antibodies starts as a co-translational step followed by a set of post-translational modifications and is considered as one of the critical quality attributes because of its impact on biological functions as well as therapy outcome. In addition to detailed product characterization of these glycans by the manufacturers, their comprehensive analysis is also a regulator ...
monoclonalantibodies, etc ; liquid-air interface; mechanical stress; microscopy; polysorbates; prediction; Show all 6 Subjects
Abstract:
... Monoclonal antibodies (mAbs) represent an important part of biological pharmaceutics. A serious challenge in their development is the formation of protein particles, which are often formed through protein aggregation at the air-liquid interface and then introduced into solution by interfacial stresses. In this paper, protein films formed at the air-liquid interface by two mAbs were disrupted by pu ...