U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject "monoclonal antibodies" Remove constraint Subject: "monoclonal antibodies"
Start Over

Search

8,648  Search Results

« Previous | 1 - 20 of 8,648 |
Number of results to display per page

Search Results

1. Analysis of residual monepantel sulfone in milk using icELISA based on a monoclonal antibody developed from an easy-synthesized and low-cost hapten

Author:
Yanfang Zhang; Pimiao Zheng; Zile Wang; Zhuoren Ling; Zhenhui Ren; Haiyang Jiang
Source:
Journal of food composition and analysis 2023 v.115 pp. 104897
ISSN:
0889-1575
Subject:
monoclonal antibodies,   etc   ; antiparasitic agents; detection limit; enzyme-linked immunosorbent assay; food composition; haptens; immunomagnetic separation; milk;     Show all 8 Subjects
Abstract:
... Monepantel (MNP) is a new class of aminoacetonitrile derivative antiparasitic drug and mainly metabolizes as monepantel sulfone in vivo. This study aimed at developing a novel monepantel sulfone (MNPS) detection method combining indirect competitive enzyme-linked immunosorbent assay (icELISA) and immunomagnetic separation. An easy-synthesized hapten was designed based on the partial structure of M ...
DOI:
10.1016/j.jfca.2022.104897

2. Production of high affinity monoclonal antibody and development of indirect competitive chemiluminescence enzyme immunoassay for gentamicin residue in animal tissues

Author:
Peng Dai; Yan Zhang; Yanping Hong; Jianhua Xiong; Huaying Du; Luying Duan; Dan Wang; Yubo Wang; Weijie Deng; Bruce D. Hammock; Wuying Yang
Source:
Food chemistry 2023 v.400 pp. 134067
ISSN:
0308-8146
Subject:
monoclonal antibodies,   etc   ; antigens; bovine serum albumin; chemiluminescence; cross reaction; detection limit; enzyme immunoassays; fish; food chemistry; gentamicin; hybridomas; pork;     Show all 12 Subjects
Abstract:
... To determine gentamicin residues in animal tissues, a monoclonal antibody (Mab) was produced and a sensitive indirect competitive chemiluminescent enzyme immunoassay (icCLEIA) was developed. At first, gentamicin was conjugated with bovine serum albumin as immunogens which were used to immunize BALB/c mice. Then, an anti-gentamicin Mab was prepared by hybridoma technology. Finally, a sensitive icCL ...
DOI:
10.1016/j.foodchem.2022.134067

3. A lateral flow immunoassay based on chemisorbed probes in virtue of hydrogen bond receptors on the Bi2S3 NPs

Author:
Huilan Hu; Yanli Tian; Xuechi Yin; Jing Ren; Lihong Su; Jingke Xu; Conghui Jia; Jianlong Wang; Daohong Zhang
Source:
Food chemistry 2023 v.401 pp. 134133
ISSN:
0308-8146
Subject:
monoclonal antibodies,   etc   ; beef; bismuth; clenbuterol; detection limit; food chemistry; food safety; hydrogen; hydrogen bonding; immunoassays; milk; nanoparticles; pork; sulfides;     Show all 14 Subjects
Abstract:
... Improving detection sensitivity is still a major research emphasis for lateral flow immunoassay (LFIA). Increasing the binding efficiency and stability of the probe is an achievable and effective solution. In this work, we developed a highly sensitive lateral flow immunoassay for clenbuterol detection by using bismuth sulfide nanoparticle (Bi₂S₃) nanoparticles (NPs) as a novel marker. Here, Bi₂S₃ ...
DOI:
10.1016/j.foodchem.2022.134133

4. Electrochemical paper-based antigen sensing platform using plant-derived monoclonal antibody for detecting SARS-CoV-2

Author:
Jutamas Jaewjaroenwattana; Waranyoo Phoolcharoen; Ekawat Pasomsub; Prinjaporn Teengam; Orawon Chailapakul
Source:
Talanta 2023 v.251 pp. 123783
ISSN:
0039-9140
Subject:
monoclonal antibodies,   etc   ; COVID-19 infection; Nicotiana benthamiana; Severe acute respiratory syndrome coronavirus 2; antigens; cellulose; cost effectiveness; detection limit; electrochemistry; electrodes; graphene; immunosensors; nanocrystals; saliva; voltammetry;     Show all 15 Subjects
Abstract:
... The current approaches of diagnostic platforms for detecting SARS-CoV-2 infections mostly relied on adapting the existing technology. In this work, a simple and low-cost electrochemical sensing platform for detecting SAR-CoV-2 antigen was established. The proposed sensor combined the innovative disposable paper-based immunosensor and cost-effective plant-based anti-SARS-CoV-2 monoclonal antibody C ...
DOI:
10.1016/j.talanta.2022.123783

5. Rapid and automatic Salmonella typhimurium detection integrating continuous-flow magnetic separation and dynamic impedance measurement

Author:
Li Xue; Ruya Guo; Nana Jin; Siyuan Wang; Hong Duan; Wuzhen Qi; Lei Wang; Yuanjin Zheng; Yanbin Li; Jianhan Lin
Source:
Food control 2023 v.143 pp. 109316
ISSN:
0956-7135
Subject:
monoclonal antibodies,   etc   ; Salmonella Typhimurium; chickens; detection limit; electrodes; food safety; gluconic acid; glucose; glucose oxidase; hydrogen peroxide; magnetic fields; magnetic separation; mobile telephones; organ-on-a-chip; polyclonal antibodies; polystyrenes;     Show all 16 Subjects
Abstract:
... Screening of pathogenic bacteria is an essential measure for ensuring food safety. A fast, sensitive and automatic platform for Salmonella determination was presented employing continuous-flow magnetic isolation, enzymatic impedance amplification and smartphone data analysis. First, magnetic nanoparticles coupling with polyclonal antibody, polystyrene spheres coupling with monoclonal antibody and ...
DOI:
10.1016/j.foodcont.2022.109316

6. Lowering the viscosity of a high-concentration antibody solution by protein–polyelectrolyte complex

Author:
Keisuke Tsumura; WeiLin Hsu; Masahiro Mimura; Aiko Horiuchi; Kentaro Shiraki
Source:
Journal of bioscience and bioengineering 2022 v.133 no.1 pp. 17-24
ISSN:
1389-1723
Subject:
monoclonal antibodies,   etc   ; antigens; phosphates; viscosity;     Show all 4 Subjects
Abstract:
... High-concentration and low-viscosity antibody formulations are necessary when administering these solutions subcutaneously (SC) due to limitations on injection volume. Here we show a method to decrease the viscosity of monoclonal antibody solution by protein–polyelectrolyte complex (PPC) with poly-l-glutamic acid (polyE). The viscosity of omalizumab solutions was 90 cP at the concentration of 150  ...
DOI:
10.1016/j.jbiosc.2021.09.011

7. Degrader-antibody conjugates

Author:
Peter S. Dragovich
Source:
Chemical Society reviews 2022 v.51 no.10 pp. 3886-3897
ISSN:
1460-4744
Subject:
monoclonal antibodies,   etc   ; cytotoxicity; ligases; proteolysis;     Show all 4 Subjects
Abstract:
... Degrader-antibody conjugates (DACs) are novel entities that combine a proteolysis targeting chimera (PROTAC) payload with a monoclonal antibody via some type of chemical linker. This review provides a current summary of the DAC field. Many general aspects associated with the creation and biological performance of traditional cytotoxic antibody-drug conjugates (ADCs) are initially presented. These ...
DOI:
10.1039/d2cs00141a

8. Ultrahigh‐speed, ultrahigh‐resolution preparative separation of protein biopharmaceuticals using membrane chromatography

Author:
Raja Ghosh
Source:
Journal of separation science 2022 v.45 no.12 pp. 2024-2033
ISSN:
1615-9306
Subject:
monoclonal antibodies,   etc   ; biopharmaceuticals; chromatography; fractionation; separation;     Show all 5 Subjects
Abstract:
... This paper discusses ultrahigh‐speed, ultrahigh‐resolution preparative protein separation using an in‐house designed membrane chromatography device. The performance of the membrane chromatography device was systematically compared with an equivalent resin‐packed preparative column. Experiments carried out using model proteins showed that membrane chromatography gave more than four times greater re ...
DOI:
10.1002/jssc.202200183

9. Dry-compression packing of hydroxyapatite nanoparticles within a flat cuboid chromatography device and its use for fast protein separation

Author:
Raja Ghosh; Geoff Hale; Yves Durocher; Paul Gatt
Source:
Journal of chromatography 2022 v.1667 pp. 462881
ISSN:
0021-9673
Subject:
monoclonal antibodies,   etc   ; cell culture; chromatography; hydroxyapatite; mammals;     Show all 5 Subjects
Abstract:
... We describe and discuss a simple dry-compression technique for preparing a flat cuboid chromatography device containing a shallow packed-bed of crystalline hydroxyapatite nanoparticles. We then discuss the use of this device for fast protein separation in the bind-and-elute mode. Such separation could be carried out at quite low pressures, making it possible to use inexpensive low pressure chromat ...
DOI:
10.1016/j.chroma.2022.462881

10. Design and optimization of Single Pass Tangential Flow Filtration for inline concentration of monoclonal antibodies

Author:
Mario G. Jabra; Andrew L. Zydney
Source:
Journal of membrane science 2022 v.643 pp. 120047
ISSN:
0376-7388
Subject:
monoclonal antibodies,   etc   ; filtrates; filtration; osmotic pressure; viscosity;     Show all 5 Subjects
Abstract:
... A number of Single Pass Tangential Flow Filtration (SPTFF) systems have recently been commercialized for inline concentration of monoclonal antibodies and other biotherapeutics, both to resolve bottlenecks in existing processes and as part of the development of integrated continuous downstream processes. The objective of this study was to examine the design and optimization of SPTFF modules using ...
DOI:
10.1016/j.memsci.2021.120047

11. Development and Structural Analysis of Antibody Therapeutics for Filoviruses

Author:
Xiaoying Yu; Erica Ollmann Saphire
Source:
Pathogens 2022 v.11 no.3 pp. -
ISSN:
2076-0817
Subject:
monoclonal antibodies,   etc   ; glycoproteins; humans; vaccine development; viruses;     Show all 5 Subjects
Abstract:
... The filoviruses, including ebolaviruses and marburgviruses, are among the world’s deadliest pathogens. As the only surface-exposed protein on mature virions, their glycoprotein GP is the focus of current therapeutic monoclonal antibody discovery efforts. With recent technological developments, potent antibodies have been identified from immunized animals and human survivors of virus infections and ...
DOI:
10.3390/pathogens11030374

12. Discovery of compounds with viscosity-reducing effects on biopharmaceutical formulations with monoclonal antibodies

Author:
Matic Proj; Mitja Zidar; Blaž Lebar; Nika Strašek; Goran Miličić; Aleš Žula; Stanislav Gobec
Source:
Computational and Structural Biotechnology Journal 2022 v.20 pp. 5420-5429
ISSN:
2001-0370
Subject:
monoclonal antibodies,   etc   ; biopharmaceuticals; biotechnology; chemistry; subcutaneous injection; viscosity;     Show all 6 Subjects
Abstract:
... For the development of concentrated monoclonal antibody formulations for subcutaneous administration, the main challenge is the high viscosity of the solutions. To compensate for this, viscosity reducing agents are commonly used as excipients. Here, we applied two computational chemistry approaches to discover new viscosity-reducing agents: fingerprint similarity searching, and physicochemical pro ...
DOI:
10.1016/j.csbj.2022.09.035

13. Immunochromatographic assay for the rapid and sensitive detection of etoxazole in orange and grape samples

Author:
Jie Liu; Xinxin Xu; Aihong Wu; Shanshan Song; Liguang Xu; Chuanlai Xu; Hua Kuang; Liqiang Liu
Source:
Lebensmittel-Wissenschaft + Technologie 2022 v.163 pp. 113519
ISSN:
0023-6438
Subject:
monoclonal antibodies,   etc   ; detection limit; etoxazole; grapes; immunoaffinity chromatography;     Show all 5 Subjects
Abstract:
... Etoxazole, a novel organofluorine pesticide, has been widely used against various mites in fruits and vegetables. In this study, we designed an etoxazole-hapten and produced a highly sensitive monoclonal antibody (mAb) against etoxazole with a half-maximal inhibitory concentration of 4.04 ng/mL. Based on the mAb, we developed an immunochromatographic assay (ICA) for the detection of etoxazole in o ...
DOI:
10.1016/j.lwt.2022.113519

14. Online optimization of dynamic binding capacity and productivity by model predictive control

Author:
Touraj Eslami; Martin Steinberger; Christian Csizmazia; Alois Jungbauer; Nico Lingg
Source:
Journal of chromatography 2022 v.1680 pp. 463420
ISSN:
0021-9673
Subject:
monoclonal antibodies,   etc   ; cation exchange chromatography; lysozyme; mechanistic models;     Show all 4 Subjects
Abstract:
... In preparative and industrial chromatography, the current viewpoint is that the dynamic binding capacity governs the process economy, and increased dynamic binding capacity and column utilization are achieved at the expense of productivity. The dynamic binding capacity in chromatography increases with residence time until it reaches a plateau, whereas productivity has an optimum. Therefore, the lo ...
DOI:
10.1016/j.chroma.2022.463420

15. Development of a broad-specific immunochromatographic assay for the screening of diclofenac and its analogs in health tea and health beverages

Author:
Xiaoxin Xu; Xinxin Xu; Shanshan Song; Liguang Xu; Yingyue Zhu; Chuanlai Xu; Hua Kuang; Liqiang Liu
Source:
Food bioscience 2022 v.50 pp. 102018
ISSN:
2212-4292
Subject:
monoclonal antibodies,   etc   ; beverages; diclofenac; immunoaffinity chromatography; quantitative analysis; tea;     Show all 6 Subjects
Abstract:
... Diclofenac (DIC) is often mixed into health products by unscrupulous merchants to claim antirheumatic effects. In this study, A highly sensitive group-selective monoclonal antibody (mAb) was prepared, and an immunochromatographic assay was developed for rapid screening of DIC, diclofenac methyl (DICA) and N-phenylanthranilic acid (PAA) in health teas and health beverages. In our quantitative analy ...
DOI:
10.1016/j.fbio.2022.102018

16. Monoclonal antibody on-rate constant determined from time-course data of ligand binding by capture ELISA: Evaluation of eight data analysis methods

Author:
Inmaculada Moreno; José Antonio Infantes; Mercedes Domínguez; Alfredo Toraño
Source:
Journal of immunological methods 2022 v.506 pp. 113292
ISSN:
0022-1759
Subject:
monoclonal antibodies,   etc   ; equations; ligands; quantitative analysis; surface plasmon resonance; toxins;     Show all 6 Subjects
Abstract:
... We describe an ELISA method with which to determine monoclonal antibody (mAb) on-rate constants (k₊₁) based on time-course data of ligand (L) binding to plate-bound mAb. The assay was performed in pseudo-first order kinetic conditions ([L] > > [mAb]) and at various starting ligand concentrations. Time-course initial velocity was analyzed by several methods to derive the pseudo-first order (kₒbₛ) a ...
DOI:
10.1016/j.jim.2022.113292

17. Simplified strategy for developing purification processes for antibody-drug conjugates using cation-exchange chromatography in flow-through mode

Author:
Rachel Hendricks; Dana Reese; Mark Fedesco; Michael Chinn; Jeffery Zhang; Matt Hutchinson
Source:
Journal of chromatography 2022 v.1666 pp. 462865
ISSN:
0021-9673
Subject:
monoclonal antibodies,   etc   ; cation exchange chromatography; hydrophobicity; molecular weight; product quality;     Show all 5 Subjects
Abstract:
... The development of manufacturing processes for antibody-drug conjugates (ADCs) presents many challenges compared to a standard monoclonal antibody. Conjugation processes typically start with an antibody intermediate that was purified to have very low levels of aggregates. The additional processing required for ADCs, including a hydrophobic small molecule and co-solvents, contributes to unacceptabl ...
DOI:
10.1016/j.chroma.2022.462865

18. The present and future of immunocytokines for cancer treatment

Author:
Dennis Y. Gout; Lotte S. Groen; Marjolein van Egmond
Source:
Cellular and molecular life sciences 2022 v.79 no.10 pp. 509
ISSN:
1420-682X
Subject:
monoclonal antibodies,   etc   ; cancer therapy; cytokines; cytotoxicity; half life; lymphoma; macrophages;     Show all 7 Subjects
Abstract:
... Monoclonal antibody (mAb) therapy has successfully been introduced as treatment of several lymphomas and leukemias. However, solid tumors reduce the efficacy of mAb therapy because of an immune-suppressive tumor micro-environment (TME), which hampers activation of effector immune cells. Pro-inflammatory cytokine therapy may counteract immune suppression in the TME and increase mAb efficacy, but un ...
DOI:
10.1007/s00018-022-04514-9

19. N-Glycosylation of monoclonal antibody therapeutics: A comprehensive review on significance and characterization

Author:
Anuj Shrivastava; Srishti Joshi; Andras Guttman; Anurag S. Rathore
Source:
Analytica chimica acta 2022 v.1209 pp. 339828
ISSN:
0003-2670
Subject:
monoclonal antibodies,   etc   ; chromatography; electrophoresis; glycopeptides; glycosylation; oligosaccharides; polysaccharides; spectroscopy; therapeutics;     Show all 9 Subjects
Abstract:
... N-glycosylation of therapeutic antibodies starts as a co-translational step followed by a set of post-translational modifications and is considered as one of the critical quality attributes because of its impact on biological functions as well as therapy outcome. In addition to detailed product characterization of these glycans by the manufacturers, their comprehensive analysis is also a regulator ...
DOI:
10.1016/j.aca.2022.339828

20. Real-time imaging of monoclonal antibody film reconstitution after mechanical stress at the air-liquid interface by Brewster angle microscopy

Author:
Timotej Žuntar; Matjaž Ličen; Drago Kuzman; Natan Osterman
Source:
Colloids and surfaces 2022 v.218 pp. 112757
ISSN:
0927-7765
Subject:
monoclonal antibodies,   etc   ; liquid-air interface; mechanical stress; microscopy; polysorbates; prediction;     Show all 6 Subjects
Abstract:
... Monoclonal antibodies (mAbs) represent an important part of biological pharmaceutics. A serious challenge in their development is the formation of protein particles, which are often formed through protein aggregation at the air-liquid interface and then introduced into solution by interfacial stresses. In this paper, protein films formed at the air-liquid interface by two mAbs were disrupted by pu ...
DOI:
10.1016/j.colsurfb.2022.112757