U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject "nucleotides" Remove constraint Subject: "nucleotides"
Start Over

Search

7,600  Search Results

« Previous | 1 - 20 of 7,600 |
Number of results to display per page

Search Results

1. Effect of 4 °C and ice temperature on umami-enhancing nucleotides of conditioned pork

Author:
Yin Zhang; Yingjie Zhang; Hui Li; Ting Bai; Qin Qian; Haichuan Peng; Yunlong Mu; Linguo Wang; Baohua Liu; Jie Chen; Zhongli Pan; Dayu Liu; Liming Zhao
Source:
Food chemistry 2023 v.401 pp. 134146
ISSN:
0308-8146
Subject:
nucleotides,   etc   ; food chemistry; glycogen; glycolysis; ham; ice; lactic acid; muscles; pyruvic acid; storage temperature; taste; umami;     Show all 12 Subjects
Abstract:
... To study new methods to delay the umami taste attenuation of the conditioned pork, the ice temperature of pork, the content of nucleotides, lactic acid, pyruvate, glycogen, and activity of key endogenous enzymes were determined for the conditioned pork stored at 4 °C and ice temperature. The results indicated that the ice temperature of the pork ham bicipital muscle was −1.4 °C. The content of uma ...
DOI:
10.1016/j.foodchem.2022.134146

2. Nuclease treatment enhances the probiotic effect of Bacillus velezensis T23 on hepatic steatosis and inflammation induced by high-fat diet in zebrafish

Author:
Fengli Zhang; Yinyin Luan; Qiang Hao; Qingshuang Zhang; Yalin Yang; Chao Ran; Zhen Zhang; Zhigang Zhou
Source:
Aquaculture 2023 v.562 pp. 738801
ISSN:
0044-8486
Subject:
nucleotides,   etc   ; Bacillus velezensis; Danio rerio; antioxidant activity; aquaculture; complement; fatty liver; fermented foods; high fat diet; immunity; inflammation; interleukin-6; intestinal microorganisms; intestines; lipogenesis; liver; lysozyme; mucus; probiotics; weight gain;     Show all 20 Subjects
Abstract:
... Metabolic diseases caused by high-fat diet (HFD) have been a major problem currently in aquaculture. Nucleotides have been used as functional nutrients to improve the growth and health of fish，such as stimulating immune responses, such as immune cells and complement lytic activity. In this experiment, we supplemented solid fermentation product of Bacillus velezensis T23 and nuclease-treated Bacill ...
DOI:
10.1016/j.aquaculture.2022.738801

3. Endogenous hydrocortisone caused metabolic perturbation and nutritional deterioration of animal-derived food in a dose-dependent manner

Author:
Wei Jia; Xin Wang; Lin Shi
Source:
Food chemistry 2023 v.401 pp. 134145
ISSN:
0308-8146
Subject:
purine nucleotides,   etc   ; animal-based foods; cortisol; domestic animals; dose response; flavor; food chemistry; glutamate-ammonia ligase; histidine; metabolomics; nitric oxide synthase; nutritive value; phenylalanine; pork; pork quality; proteomics; public health; toxicity; transcription (genetics);     Show all 19 Subjects
Abstract:
... Endogenous hydrocortisone causes detrimental effects on public health and domestic animal products, but the potential mechanisms remain elusive. Hydrocortisone was detected from seventy-two Guanzhong-Black pigs in three replicates (216 samples) (0.00 ± 46.38 μg kg⁻¹), indicating the existence of endogenous hydrocortisone. Herein, we investigated the effects of hydrocortisone on the metabolic signa ...
DOI:
10.1016/j.foodchem.2022.134145

4. Improved sperm DNA integrity and altered fat metabolism and intestinal morphology in Nile tilapia (Oreochromis niloticus) males fed rations with purified nucleotide

Author:
Robie Allan Bombardelli; Juliano Kelvin dos Santos Henriques; Marta Margarete Cestari; Antônio Ernesto Meister Luz Marques; Taís Voelkl Chagas; Fabio Meurer
Source:
Aquaculture 2023 v.562 pp. 738682
ISSN:
0044-8486
Subject:
nucleotides,   etc   ; DNA; Oreochromis niloticus; aquaculture; blood glucose; chromatin; diet; digestible energy; digestible protein; enterocytes; fish; gonadosomatic index; hepatosomatic index; high density lipoprotein; lipid content; lipid metabolism; liver; morphometry; muscles; testes; triacylglycerols; visceral fat; viscosity;     Show all 23 Subjects
Abstract:
... In this study, we assessed the health, growth, sperm characteristics, and intestinal, liver, and testicular morphology of Nile tilapia (Oreochromis niloticus) males fed rations containing purified nucleotides (NT). A total of 420 females (286 ± 7.15 g) and 140 males (536 ± 13.4 g) were used. Fish were fed on extruded ration (280 g digestible protein kg⁻¹; 11.72 M joule (MJ) of digestible energy kg ...
DOI:
10.1016/j.aquaculture.2022.738682

5. Analysis of pseudouridines and other RNA modifications using HydraPsiSeq protocol

Author:
Virginie Marchand; Valérie Bourguignon-Igel; Mark Helm; Yuri Motorin
Source:
Methods 2022 v.203 pp. 383-391
ISSN:
1046-2023
Subject:
nucleotides,   etc   ; RNA; hydrazine;     Show all 3 Subjects
Abstract:
... Detection of RNA modified nucleotides using deep sequencing can be performed by several approaches, including antibody-driven enrichment and natural or chemically induced RT signatures. However, only very few RNA modified nucleotides generate natural RT signatures and antibody-driven enrichment heavily depends on the quality of antibodies used and may be highly biased. Thus, the use of chemically- ...
DOI:
10.1016/j.ymeth.2021.08.008

6. Circulating miRNA as potential biomarkers for diabetes mellitus type 2: should we focus on searching for sex differences?

Author:
Weronika Kraczkowska; Lucyna Stachowiak; Andrzej Pławski; Paweł Piotr Jagodziński
Source:
Journal of applied genetics 2022 v.63 no.2 pp. 293-303
ISSN:
1234-1983
Subject:
nucleotides,   etc   ; biomarkers; microRNA; pathogenesis;     Show all 4 Subjects
Abstract:
... microRNAs are non-coding molecules, approximately 22 nucleotides in length, that regulate various cellular processes. A growing body of evidence has suggested that their dysregulated expression is involved in the pathogenesis of diverse diseases, including diabetes mellitus type 2 (DM2). Early onset of this chronic and complex metabolic disorder is frequently undiagnosed, leading to the developmen ...
DOI:
10.1007/s13353-021-00678-5

7. Predicting RNA solvent accessibility from multi-scale context feature via multi-shot neural network

Author:
Xue-Qiang Fan; Jun Hu; Yu-Xuan Tang; Ning-Xin Jia; Dong-Jun Yu; Gui-Jun Zhang
Source:
Analytical biochemistry 2022 v.654 pp. 114802
ISSN:
0003-2697
Subject:
nucleotides,   etc   ; RNA; prediction; solvents;     Show all 4 Subjects
Abstract:
... Knowledge of RNA solvent accessibility has recently become attractive due to the increasing awareness of its importance for key biological process. Accurately predicting the solvent accessibility of RNA is crucial for understanding its 3D structure and biological function. In this study, we develop a novel computational method, termed M²pred, for accurately predicting the solvent accessibility of ...
DOI:
10.1016/j.ab.2022.114802

8. In planta vs viral expression of HCPro affects its binding of nonplant 21–22 nucleotide small RNAs, but not its preference for 5′‐terminal adenines, or its effects on small RNA methylation

Author:
Francisco del Toro; Hao Sun; Carmen Robinson; Álvaro Jiménez; Eva Covielles; Tomás Higuera; Emmanuel Aguilar; Francisco Tenllado; Tomás Canto
Source:
The new phytologist 2022 v.233 no.5 pp. 2266-2281
ISSN:
0028-646X
Subject:
nucleotides,   etc   ; Potyvirus; RNA; adenines; methylation;     Show all 5 Subjects
Abstract:
... Previous studies have found a correlation between the abilities of PVX vector‐expressed HCPro variants to bind small RNAs (sRNAs), and to suppress silencing. Moreover, HCPro preferred to bind viral sRNAs of 21–22 nucleotides (nt) containing 5′‐terminal adenines. This would require such viral sRNAs to have either different access to the suppressor than those of plant sequences, or different molecul ...
DOI:
10.1111/nph.17935

9. MicroRNA turnover: a tale of tailing, trimming, and targets

Author:
Jaeil Han; Joshua T. Mendell
Source:
Trends in biochemical sciences 2022 pp. -
ISSN:
0968-0004
Subject:
nucleotides,   etc   ; biogenesis; gene expression; microRNA;     Show all 4 Subjects
Abstract:
... MicroRNAs (miRNAs) post-transcriptionally repress gene expression by guiding Argonaute (AGO) proteins to target mRNAs. While much is known about the regulation of miRNA biogenesis, miRNA degradation pathways are comparatively poorly understood. Although miRNAs generally exhibit slow turnover, they can be rapidly degraded through regulated mechanisms that act in a context- or sequence-specific mann ...
DOI:
10.1016/j.tibs.2022.06.005

10. Simultaneous visualization of the dynamics of crosslinked and single microtubules In Vitro by tirf microscopy

Author:
Mani Nandini; Marchan Michelle F.; Subramanian Radhika
Source:
Journal of visualized experiments 2022 no.180 pp. e63377
ISSN:
1940-087X
Subject:
nucleotides,   etc   ; crosslinking; microscopy; microtubules; tubulin;     Show all 5 Subjects
Abstract:
... Microtubules are polymers of αβ-tubulin heterodimers that organize into distinct structures in cells. Microtubule-based architectures and networks often contain subsets of microtubule arrays that differ in their dynamic properties. For example, in dividing cells, stable bundles of crosslinked microtubules coexist in close proximity to dynamic non-crosslinked microtubules. TIRF-microscopy-based in ...
DOI:
10.3791/63377

11. Circulating microRNAs as the Potential Diagnostic and Prognostic Biomarkers for Nasopharyngeal Carcinoma

Author:
Thuy Ai Huyen Le; Thuan Duc Lao
Source:
Genes 2022 v.13 no.7 pp. -
ISSN:
2073-4425
Subject:
nucleotides,   etc   ; biomarkers; carcinoma; microRNA; prognosis;     Show all 5 Subjects
Abstract:
... microRNAs are endogenous non-coding miRNAs, 19–25 nucleotides in length, that can be detected in the extracellular environment in stable forms, named circulating miRNAs (CIR-miRNAs). Since the first discovery of CIR-miRNAs, a large number of studies have demonstrated that the abnormal changes in its expression could be used to significantly distinguish nasopharyngeal carcinoma (NPC) from healthy c ...
DOI:
10.3390/genes13071160

12. Reconstruction of the rRNA Sequences of LUCA, with Bioinformatic Implication of the Local Similarities Shared by Them

Author:
Yu Men; Guoliang Lu; Yanhui Wang; Jinzhong Lin; Qiang Xie
Source:
Biology 2022 v.11 no.6 pp. -
ISSN:
2079-7737
Subject:
nucleotides,   etc   ; ancestry; bioinformatics; phylogeny; ribosomes;     Show all 5 Subjects
Abstract:
... The theory of the RNA world, especially with the catalytic capability of RNA, provides a reasonable framework explaining the evolution of molecular genetics system before the scenario of the central dogma. However, it remains a challenge to deduce the origin mechanism of rRNAs. Here we reconstructed the phylogenetic relationships of archaea and bacteria with bootstrap values of most nodes, especia ...
DOI:
10.3390/biology11060837

13. The siRNA Off-Target Effect Is Determined by Base-Pairing Stabilities of Two Different Regions with Opposite Effects

Author:
Yoshiaki Kobayashi; Shen Tian; Kumiko Ui-Tei
Source:
Genes 2022 v.13 no.2 pp. -
ISSN:
2073-4425
Subject:
nucleotides,   etc   ; RNA interference; microRNA; thermodynamics;     Show all 4 Subjects
Abstract:
... In RNA interference (RNAi), small interfering RNA (siRNA) suppresses the expression of its target mRNA with a perfect complementary sequence. In addition, siRNA also suppresses the expression of unintended mRNAs with partially complementary sequences mainly within the siRNA seed region (nucleotides 2–8). This mechanism is highly similar to microRNA (miRNA)-mediated RNA silencing, and known as the ...
DOI:
10.3390/genes13020319

14. Complete genome sequence of GII.9 norovirus

Author:
Zilong Zhang; Danlei Liu; Zilei Zhang; Peng Tian; Shenwei Li; Qingping Wu; Dapeng Wang; Zhengan Tian
Source:
Archives of virology 2022 v.167 no.1 pp. 249-253
ISSN:
0304-8608
Subject:
nucleotides,   etc   ; Norovirus; gastroenteritis; genotype; patients; virology;     Show all 6 Subjects
Abstract:
... Norovirus is recognized as one of the leading causes of acute gastroenteritis outbreaks. Genotype GII.9 was first detected in Norfolk, VA, USA, in 1997. However, the complete genome sequence of this genotype has not yet been determined. In this study, a complete genome sequence of GII.9[P7] norovirus, SCD1878_GII.9[P7], from a patient was determined using high-throughput sequencing and rapid ampli ...
DOI:
10.1007/s00705-021-05257-x

15. Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA

Author:
Jory Lietard; Dominik Ameur; Mark M. Somoza
Source:
RSC advances 2022 v.12 no.9 pp. 5629-5637
ISSN:
2046-2069
Subject:
nucleotides,   etc   ; DNA; fluorescein; fluorescence; guanine; redox potential;     Show all 6 Subjects
Abstract:
... Fluorescein is commonly used to label macromolecules, particularly proteins and nucleic acids, but its fluorescence is known to be strongly dependent on its direct chemical environment. In the case of fluorescein-labeled nucleic acids, nucleobase-specific quenching originating in photoinduced charge transfer interactions results in sequence-dependent chemical environments. The resulting sequence s ...
DOI:
10.1039/d2ra00534d

16. Visualizing “Alternative Isoinformational Engineered” DNA in A- and B-Forms at High Resolution

Author:
Shuichi Hoshika; Madhura S. Shukla; Steven A. Benner; Millie M. Georgiadis
Source:
Journal of the American Chemical Society 2022 v.144 no.34 pp. 15603-15611
ISSN:
1520-5126
Subject:
nucleotides,   etc   ; Z-DNA; biomimetics; geometry; nucleobases; polymers;     Show all 6 Subjects
Abstract:
... A fundamental property of DNA built from four informational nucleotide units (GCAT) is its ability to adopt different helical forms within the context of the Watson–Crick pair. Well-characterized examples include A-, B-, and Z-DNA. For this study, we created an isoinformational biomimetic polymer, built (like standard DNA) from four informational “letters”, but with the building blocks being artif ...
DOI:
10.1021/jacs.2c05255

17. DYW deaminase domain has a distinct preference for neighboring nucleotides of the target RNA editing sites

Author:
Ayako Maeda; Sachi Takenaka; Tenghua Wang; Brody Frink; Toshiharu Shikanai; Mizuki Takenaka
Source:
plant journal 2022 v.111 no.3 pp. 756-767
ISSN:
0960-7412
Subject:
nucleotides,   etc   ; Escherichia coli; Physcomitrium; RNA; cytidine; deamination; organelles;     Show all 7 Subjects
Abstract:
... C‐to‐U RNA editing sites in plant organelles show a strong bias for neighboring nucleotides. The nucleotide upstream of the target cytidine is typically C or U, whereas A and G are less common and rare, respectively. In pentatricopeptide repeat (PPR)‐type RNA editing factors, the PPR domain specifically binds to the 5′ sequence of target cytidines, whereas the DYW domain catalyzes the C‐to‐U deami ...
DOI:
10.1111/tpj.15850

18. TLR9: A friend or a foe

Author:
Mona M. Saber; Nada Monir; Azza S. Awad; Marwa E. Elsherbiny; Hala F. Zaki
Source:
Life sciences 2022 v.307 pp. 120874
ISSN:
0024-3205
Subject:
nucleotides,   etc   ; homeostasis; immune system; inflammation; innate immunity; therapeutics;     Show all 6 Subjects
Abstract:
... The innate immune system is a primary protective line in our body. It confers its protection through different pattern recognition receptors (PRRs), especially toll like receptors (TLRs). Toll like receptor 9 (TLR9) is an intracellular TLR, expressed in different immunological and non-immunological cells. Release of cellular components, such as proteins, nucleotides, and DNA confers a beneficial i ...
DOI:
10.1016/j.lfs.2022.120874

19. Creation, Optimization, and Use of Semi-Synthetic Organisms that Store and Retrieve Increased Genetic Information

Author:
Floyd E. Romesberg
Source:
Journal of molecular biology 2022 v.434 no.8 pp. 167331
ISSN:
0022-2836
Subject:
nucleotides,   etc   ; RNA; anticodons; codons; genetic code; molecular biology; therapeutics;     Show all 7 Subjects
Abstract:
... With few exceptions, natural proteins are built from only 20 canonical (proteogenic) amino acids which limits the functionality and accordingly the properties they can possess. Genetic code expansion, i.e. the creation of codons and the machinery needed to assign them to non-canonical amino acids (ncAAs), promises to enable the discovery of proteins with novel properties that are otherwise difficu ...
DOI:
10.1016/j.jmb.2021.167331

20. Structural Assembly of Qβ Virion and Its Diverse Forms of Virus-like Particles

Author:
Jeng-Yih Chang; Karl V. Gorzelnik; Jirapat Thongchol; Junjie Zhang
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
nucleotides,   etc   ; Leviviridae; RNA; biotechnology; capsid; fimbriae; genomics; models; virion;     Show all 9 Subjects
Abstract:
... The coat proteins (CPs) of single-stranded RNA bacteriophages (ssRNA phages) directly assemble around the genomic RNA (gRNA) to form a near-icosahedral capsid with a single maturation protein (Mat) that binds the gRNA and interacts with the retractile pilus during infection of the host. Understanding the assembly of ssRNA phages is essential for their use in biotechnology, such as RNA protection a ...
DOI:
10.3390/v14020225