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openreadingframes, etc ; Bacteroides; Firmicutes; Macrotermes; cellulose; clean energy; databases; energy; glycosides; humans; hydrolases; insects; intestines; metagenomics; renewable energy sources; research; Show all 16 Subjects
Abstract:
... Energy supply is a major concern of human survival and development, and that concern demands new sources of renewable clean energy. Cellulose, as a renewable energy material is affected by low activity and high cost of cellulose. Studies of cellulose degrading bacterial microbiota in insect intestine have revealed that these potential microbes can be used as a rich resource of cellulose degrading ...
Sk Sarif Hassan; Pallab Basu; Elrashdy M. Redwan; Kenneth Lundstrom; Pabitra Pal Choudhury; Ángel Serrano-Aroca; Gajendra Kumar Azad; Alaa A.A. Aljabali; Giorgio Palu; Tarek Mohamed Abd El-Aziz; Debmalya Barh; Bruce D. Uhal; Parise Adadi; Kazuo Takayama; Nicolas G. Bazan; Murtaza M. Tambuwala; Amos Lal; Gaurav Chauhan; Wagner Baetas-da-Cruz; Samendra P. Sherchan; Vladimir N. Uversky
openreadingframes, etc ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; amino acids; asymmetry; evolution; pandemic; research; time series analysis; uncertainty; viruses; Brazil; India; Show all 13 Subjects
Abstract:
... Various lineages of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) have contributed to prolongation of the Coronavirus Disease 2019 (COVID-19) pandemic. Several non-synonymous mutations in SARS-CoV-2 proteins have generated multiple SARS-CoV-2 variants. In our previous report, we have shown that an evenly uneven distribution of unique protein variants of SARS-CoV-2 is geo-location or ...
openreadingframes, etc ; Cynoglossus semilaevis; aquaculture; brain; females; genes; insulin-like growth factor binding proteins; liver; males; methylation; phylogeny; prediction; reproduction; sex reversal; sexual dimorphism; testosterone; Show all 16 Subjects
Abstract:
... Chinese tongue sole (Cynoglossus semilaevis) is an economically important marine flatfish that shows typical female-biased Sexual Size Dimorphism (SSD) and sex reversal from female to pseudomale. Previous studies have shown that the insulin-like growth factor-binding protein (igfbp) genes are differentially expressed between sexes of C. semilaevis. The igfbp family is one of the important members ...
openreadingframes, etc ; Infectious spleen and kidney necrosis virus; Rhabdoviridae; Siniperca chuatsi; amino acid sequences; brain; fish; glutamine; immunology; kidneys; metabolism; protein content; shellfish; spleen; transcription factors; virus replication; Show all 16 Subjects
Abstract:
... c-Myc is a transcription factor and master regulator of cellular metabolism, and plays a critical role in virus replication by regulating glutamine metabolism. In this study, the open-reading frame (ORF) of c-Myc, designated as Sc-c-Myc, was cloned and sequenced. Multiple alignment of the amino acid sequence showed that the conserved domain of Sc-c-Myc, including the helix–loop–helix-zipper (bHLHz ...
openreadingframes, etc ; Escherichia coli; Orthohepevirus A; epitopes; immunogenicity; swine; Show all 6 Subjects
Abstract:
... To evaluate the antigenic properties of Hepatitis E Virus (HEV) Open Reading Frame 2 and 3 (ORF2 and ORF3) codified proteins, we expressed different portions of ORF2 and the entire ORF3 in E. coli, a truncated ORF2, was also expressed in baculovirus. A panel of 37 monoclonal antibodies (MAbs) was raised against ORF2 (1–660 amino acids) and MAbs were mapped and characterized using the ORF2 expresse ...
openreadingframes, etc ; evolution; genetic engineering; heavy metals; humans; peptides; retrotransposons; Show all 7 Subjects
Abstract:
... The human long interspersed nuclear element 1 (LINE1) has been implicated in numerous diseases and has been suggested to play a significant role in genetic evolution. Open reading frame 1 protein (ORF1p) is one of the two proteins encoded in this self-replicating mobile genetic element, both of which are essential for retrotransposition. The structure of the three-stranded coiled-coil domain of OR ...
openreadingframes, etc ; Dicistroviridae; endoparasitoids; genome; length; new species; nucleotides; phylogeny; polyproteins; tail; virology; viruses; wasps; Show all 13 Subjects
Abstract:
... Here, we report a novel RNA virus from an encyrtid endoparasitoid wasp (Diversinervus elegans). This virus has a genome of 8845 nucleotides in length with a poly(A) tail. It contains one open reading frame (ORF) encoding a single polyprotein that shares the most significant similarity to the polyproteins of dicistroviruses. Phylogenetic analysis suggested that this virus belongs to the family Dici ...
openreadingframes, etc ; Arabidopsis; Phaseolus vulgaris; binding proteins; botany; genes; polyribosomes; protein synthesis; transcription (genetics); transgenic plants; water; Show all 11 Subjects
Abstract:
... To assure an efficient protein production under stress conditions, some transcripts are modulated at the translational level. To better understand the control of protein production under stress, we analysed the enhancing effect of the 3′ untranslated region (UTR) of the water deficit responsive gene PvLEA6 from Phaseolus vulgaris on the regulation of its expression. GUS activity and transcript loa ...
openreadingframes, etc ; adhesion; desmosomes; epithelium; genomics; glycoproteins; histopathology; homozygosity; keratin; loss-of-function mutation; phenotype; stop codon; Show all 12 Subjects
Abstract:
... Investigations of hereditary phenotypes in spontaneous mutants may help to better understand the physiological functions of the altered genes. We investigated two unrelated domestic shorthair cats with bulbous swellings of the hair shafts. The clinical, histopathological, and ultrastructural features were similar to those in mice with lanceolate hair phenotype caused by loss-of-function variants i ...
openreadingframes, etc ; Methanosarcina mazei; disulfide bonds; genome; molecular weight; peptides; post-translational modification; protein products; proteome; proteomics; solid phase extraction; Show all 11 Subjects
Abstract:
... Short open reading frame-encoded peptides (SEP) represent a widely undiscovered part of the proteome. The detailed analysis of SEP has, despite inherent limitations such as incomplete sequence coverage, challenges encountered with protein inference, the identification of posttranslational modifications and the assignment of potential N- and C-terminal truncations, predominantly been assessed using ...
openreadingframes, etc ; Gammaherpesvirinae; peptides; progeny; virion; virus replication; Show all 6 Subjects
Abstract:
... The Open Reading Frame 45 (ORF45) of Kaposi sarcoma-associated herpesvirus (KSHV) is a gammaherpesvirus-specific, immediate-early, tegument protein required for efficient viral replication and virion production. We have previously shown that ORF45 interacts with the conserved herpesviral protein ORF33 through the highly conserved C-terminal 19 amino acids (C19) of ORF45. Because the deletion of C1 ...
openreadingframes, etc ; amino acids; cell lines; databases; genome; humans; molecular weight; neoplasms; non-coding RNA; peptides; peptidomics; proteins; proteome; solvents; tandem mass spectrometry; Show all 15 Subjects
Abstract:
... The small proteins and short open reading frames encoded peptides (SEPs) are of fundamental importance because of their essential roles in biological processes. However, the annotation or identification of them is challenging, in part owing to the limitation of the traditional genome annotation pipeline and their inherent characteristics of low abundance and low molecular weight. To discover and c ...
... Cytochalasins are a group of fungal secondary metabolites with diverse structures and bioactivities, including chaetoglobosin A production. Chaetoglobosin A is produced by Chaetomium globosum and has potential antifungal activity. Bioinformatics analysis of the chaetoglobosin A gene cluster (che) showed it that consists of nine open reading frames, including those encoding polyketide synthases (PK ...
openreadingframes, etc ; RNA-binding proteins; Scylla paramamosain; T-lymphocytes; White spot syndrome virus; apoptosis; caspase-3; crabs; hemocytes; immune response; innate immunity; membrane potential; mitochondrial membrane; Show all 13 Subjects
Abstract:
... TIA-1 (T cell restricted intracellular antigen-1) is a kind of RNA-binding protein which serves as the downstream of CED-9 (a BCL2 homolog) and induces apoptosis under stress conditions. So far, the function of apoptosis mediated by TIA-1 has been extensively studied in higher animals, and apoptosis happens to be related to biological immune defense. However, the involvement of TIA-1 in the study ...
... Autophagy is regulated by a variety of autophagy related genes (ATG), among them, ATG4 is a highly specific protease that is critical to autophagy. The purpose of this study was to investigate the role of the ATG4 response to Palmitic acid (PA) and their relationships with Endoplasmic reticulum (ER) stress in grass carp. In the present study, four ATG4 paralogs from grass carp were identified and ...
... Brucella is an intracellular zoonotic pathogen that can affect many hosts. Brucella melitensis Rev.1 is a live attenuated, is one of the most effective vaccine strain against brucellosis. It can be used safely in sheep, goats, and even cattle. Although many studies are available on this topic, there is no effective vaccine strain for sheep and goats that distinguishes the antibody titer produced b ...
... Arginine kinase (AK, EC 2.7.3.3) plays an important role in cells with high, fluctuating energy requirements. In invertebrates, AK is the major phosphagen kinase that modulates the energy metabolism. Here, the full-length cDNA sequence encoding arginine kinase (EcAK) was obtained from the Exopalaemon carinicauda. The complete nucleotide sequence of EcAK contained a 1068 bp open reading frame (ORF) ...
openreadingframes, etc ; Arabidopsis thaliana; Prunus yedoensis; amino acid sequences; mitochondria; mutants; nucleotides; peroxidase; plant growth; proline; reactive oxygen species; salt stress; salt tolerance; sequence homology; stress tolerance; superoxide dismutase; Show all 16 Subjects
Abstract:
... Alternative oxidase (AOX) has a well-established involvement in plant growth and stress tolerance in many studies. In this study, we isolated and characterized the AOX2 from Cerasus humilis. The ChAOX2 Open Reading Frame (ORF) contains 1029 nucleotides and encodes 342 amino acid residues. The inferred amino acid sequence of ChAOX2 shared the highest sequence similarity with a homolog from Prunus y ...
Pasquale Marmo; Maria Cristina Thaller; Gustavo Di Lallo; Lucia Henrici De Angelis; Noemi Poerio; Federica De Santis; Maurizio Fraziano; Luciana Migliore; Marco Maria D’Andrea
openreadingframes, etc ; DNA; Siphoviridae; Sphingomonas; bacteriophages; bioremediation; biosynthesis; host range; multigene family; pH; temperature; Show all 11 Subjects
Abstract:
... Members of Sphingomonas genus have gained a notable interest for their use in a wide range of biotechnological applications, ranging from bioremediation to the production of valuable compounds of industrial interest. To date, knowledge on phages targeting Sphingomonas spp. are still scarce. Here, we describe and characterize a lytic bacteriophage, named vB_StuS_MMDA13, able to infect the Sphingomo ...
openreadingframes, etc ; Aphidoidea; Citrus; Citrus tristeza virus; genetic variation; genome; genotype; grapefruits; pathogenicity; proteinases; Show all 10 Subjects
Abstract:
... Two isolates of the T68 genotype of citrus tristeza virus (CTV) were derived from a common source, GFMS12, by single aphid transmission. These isolates, named GFMS12-8 and GFMS12-1.3, induced stem pitting with differing severity in ‘Duncan’ grapefruit (Citrus × paradisi [Macfad.]). Full-genome sequencing of these isolates showed only minor nucleotide sequence differences totaling 45 polymorphisms. ...