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organculture, etc ; bananas; micropropagation; plant growth; plantlets; Show all 5 Subjects
Abstract:
... Continues subculturing of in vitro plant subcultures is essential for commercial micropropagation and tissue culture research. In unusual situations, the subcultures can be delayed, and the slow-growth in vitro storage technic could be applied to reduce the loss of plant material. The present study aimed to evaluate the slow-growth in vitro storage of banana plantlets (‘Prata Catarina’; group AAB) ...
organculture, etc ; biochemical pathways; metabolism; secondary metabolites; transcription factors; Show all 5 Subjects
Abstract:
... The active ingredients of many Chinese medicinal plants are secondary metabolites, which exhibit pharmaceutical value. In the metabolism process of secondary metabolites, transcription factors play important roles in regulating a series of genes in the metabolic pathway, thereby regulating the direction and the speed of metabolic flow. Therefore, the intervention of transcription factor by genetic ...
organculture, etc ; Panax; dietary supplements; markets; paclitaxel; patients; therapeutics; Show all 7 Subjects
Abstract:
... Few therapeutic specialty molecules from in vitro cultures beyond paclitaxel have come to market and although other more complex products like ginseng have also appeared, success has been limited. Often it is not the science that is limiting, but rather regulatory issues that limit considerations of potential products mainly because of costs in getting the product to market. Here we discuss broade ...
organculture, etc ; Arabidopsis thaliana; Vigna radiata; loci; mung beans; soybeans; Show all 6 Subjects
Abstract:
... CONSTANS-LIKE (COL) genes are important factors that play an important role in regulating photoperiod-dependent flowering time, and multiple COL genes have been identified in plants. However, knowledge of the COLs involved in photoperiod-dependent flowering time regulation is limited in mungbean (Vigna radiata). In this study, we characterized the function of VrCOL1 in regulating flowering time. V ...
organculture, etc ; cornea; fibroblast growth factor 1; humans; surgery; vision; Show all 6 Subjects
Abstract:
... Fuchs Endothelial Corneal Dystrophy (FECD) results from dysfunctional corneal endothelial cells (CECs) and is currently treated by transplantation of the whole cornea or Descemet's membrane. Recent developments in ocular surgery have established Descemet's Stripping Only (DSO), a surgical technique in which a central circle of guttae-dense Descemet's membrane is removed to allow for the migration ...
organculture, etc ; cultivars; gain-of-function mutation; genomics; phenotype; potatoes; Show all 6 Subjects
Abstract:
... Potato is one of the most important crops grown worldwide. The potato genome sequencing consortium has identified large numbers of genes having unknown functionality. To systematically assign functions to all predicted genes in its genome and their specific applications in potato improvement, different techniques have been developed over the years to generate mutants and analyze phenotypic variati ...
organculture, etc ; Phoenix dactylifera; callus; plantlets; zinc; zinc oxide; zinc sulfate; Show all 7 Subjects
Abstract:
... Nanotechnology is a rapidly spreading field to be applied in agriculture. From all kinds of nutrients, zinc nanoparticles had a great interest in investigations due to its importance for plant as it involved in many growth processes. Presently, rare literature is available about nanoparticles effects on date palm in vitro propagation, especially zinc oxide nanoparticles (ZnO-NPs). Therefore, ZnO-N ...
organculture, etc ; Apocynaceae; genetic distance; human health; micropropagation; nucleotide sequences; Vietnam; Show all 7 Subjects
Abstract:
... Jasminanthes tuyetanhiae is one of the precious herbs and good for human health, discovered in the Da-Bia Mountain (Phu Yen province, Vietnam). The identification of the CTW species (species believed to be J. tuyetanhiae collected in 2019) by molecular indicators showed that this species belongs to the Apocynaceae family. High similarity (98.2%) in the ITS1-5.8SrRNA-ITS2 gene sequence of CTW when ...
organculture, etc ; Crinum; benzyladenine; medicinal plants; microscopy; morphometry; plantlets; rivers; survival rate; Show all 9 Subjects
Abstract:
... The current investigation revealed a highly proficient and reproducible method for comprehensive in vitro regeneration and structural differentiation of bulblets in Crinum malabaricum Lekhak & Yadav using meta-topolin [mT; 6-(3-hydroxybenzylamino) purine] [6-benzylaminopurine] for the first time. Cultures were established from twin scales explants using optimized sterilization method and growth re ...
organculture, etc ; Cyclocarya paliurus; anthocyanins; biosynthesis; cell culture; metabolome; metabolomics; oxidoreductases; transcriptome; Show all 9 Subjects
Abstract:
... Plant cell culture is a good way to produce anthocyanins owing to its outstanding advantages of short cycles and no limitation on natural environments. However, this method has not been widely used due to the lack of elite cell lines, low yield, and relatively high cost. In the present paper, suspension culture technology of red Cyclocarya paliurus cells was developed to produce anthocyanins, and ...
organculture, etc ; callus; genetic stability; leaves; organogenesis; plant growth; rooting; survival rate; Show all 8 Subjects
Abstract:
... An efficient indirect organogenesis protocol for Naregamia alata was standardized in the present investigation. Purified form of vermicast extract (VCE) was used in combination with various plant growth regulators for callus induction, shoot regeneration, and root induction. Leaf explants were inoculated on MS medium with various concentrations (1.0–2.5 mg L⁻¹) of 2, 4-D and NAA combined with 20%, ...
organculture, etc ; Panax ginseng; abscisic acid; biosynthesis; cold stress; elicitors; ginsenosides; nordihydroguaiaretic acid; Show all 8 Subjects
Abstract:
... Ginsenosides Rg₁ and Re are two major protopanaxatriol-type ginsenosides in Panax ginseng. Dammarendiol synthase (DDS) and protopanaxatriol synthase (PPTS) are the two key enzymes in the biosynthesis of PPT-type ginsenosides. Abscisic acid (ABA) is an important endogenous phytohormone, which is involved in abiotic stress. P. ginseng adventitious roots (PGARs) are attractive resources to be used in ...
organculture, etc ; Ocimum; basil; cultivars; flavonoids; genotype; plant tissues; secondary metabolites; somatic embryogenesis; Show all 9 Subjects
Abstract:
... Species from the genus Ocimum L. (basil) are among the most cultivated plants due to their large content of secondary metabolites, in particular essential oils and phenolic compounds. Still, different conditions of basil cultivation cause significant variations of quantitative and qualitative characteristics of material originating from basil plants. The application of plant tissue culture to prod ...
organculture, etc ; Withania somnifera; chemical constituents of plants; cultivars; medicinal plants; secondary metabolites; Show all 6 Subjects
Abstract:
... Withania somnifera (L.) Dunal is a valuable medicinal plant in the Solanaceae family. It is commonly known as Ashwagandha and is widely distributed around the globe. It has multiple pharmacological properties owing to the existence of diverse secondary metabolites viz., withanolide A, withanolide D, withaferin A, and withanone. It is in great demand in the herbal industry because of its extensive ...
Franciele Pereira Rossini; João Paulo Rodrigues Martins; Samuel Werner Moreira; Lorenzo Toscano Conde; Evens Clairvil; Priscila da Conceição de Souza Braga; Antelmo Ralph Falqueto; Andreia Barcelos Passos Lima Gontijo
organculture, etc ; Alternanthera ficoidea; aerial parts; bioaccumulation; biomass production; cadmium; photosynthesis; phytoremediation; silicon; Show all 9 Subjects
Abstract:
... Despite having the ability to bioaccumulate trace elements such as cadmium (Cd), many species also present morphophysiological disorders that can hamper their use as phytoremediation plants. Since it can lead to alterations in biomass accumulation, the employment of elements that mitigate stress, such as silicon (Si), can diminish the deleterious effects caused by trace elements. The objective of ...
organculture, etc ; Hevea brasiliensis; colchicine; diploidy; germplasm; leaves; liquids; stress tolerance; tetraploidy; triploidy; Show all 10 Subjects
Abstract:
... Polyploidy is a potential way to breed new varieties with high yield and stress resistance in plants. In this study, we provided an efficient method for induction of tetraploid of Hevea brasiliensis by immerse somatic embryos in the liquid plant induction medium containing colchicine. The optimum concentration of colchicine is 5 mg/ml, and the optimum immersion time is 24–48 h. The highest average ...
organculture, etc ; Nicotiana tabacum; flowers; landscapes; leaves; mitochondria; mitochondrial RNA; sequence analysis; tobacco; Show all 9 Subjects
Abstract:
... RNA editing is a prevalent nucleotide modification at the RNA level in higher plants. However, little is known about the dynamic distribution of RNA editing among tissues. In this study, we explored the tissue specificity of mitochondrial RNA editing in tobacco (Nicotiana tabacum) based on publicly available RNA-seq data from four tobacco tissues: root, stem, leaf, and flower. As a result, 473 RNA ...
organculture, etc ; Epidendrum; callus; endopolyploidy; flow cytometry; leaves; micropropagation; oxidation; plantlets; pollinia (pollen); Show all 10 Subjects
Abstract:
... The induction and regeneration of protocorm-like bodies (PLBs) is a morphogenetic pathway widely used for orchid micropropagation. As endopolyploidy, i.e., the coexistence of cells with different ploidy levels, is a common feature in orchid tissues, a natural question arises when using somatic tissues as explants for orchid micropropagation: does endopolyploidy in explants affect the cytogenetic s ...
organculture, etc ; azadirachtin; cryopreservation; genes; organogenesis; paclitaxel; plant tissues; secondary metabolites; somatic embryogenesis; trees; Show all 10 Subjects
Abstract:
... Many tree species are an excellent source of a wide range of bioactive compounds of pharmaceutical importance. However, overexploitation of medicinal trees to fulfil the demand for plant-based herbal drugs puts pressure on its natural population. Therefore, many tree species are declining continuously, particularly those used in pharmaceutical industries. In addition, limited production of seconda ...
organculture, etc ; drought tolerance; gene expression; genes; phenotype; tomatoes; water stress; zinc finger motif; Show all 8 Subjects
Abstract:
... SLZF57 belongs to the C2H2 zinc finger protein family. Our group’s previous experiments found that SLZF57 was active in response to drought stress. In this experiment, SLZF57 was analyzed for the ABA hormone-induced expression pattern, and VIGS technology was used to verify the function of SLZF57. The experimental results showed that the expression of SLZF57 was upregulated after the induction of ...