An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
... The study aimed to identify umami peptides from Boletus edulis and explore their umami mechanism. 421, 713 and 616 peptides identified by LC-MS/MS from control sample (CS), enzymatically extracted sample (EES) and high-pressure cooking sample (HPCS), respectively. According to molecular docking study, three potential umami peptides (DGF, KCGQ and HHYE) were chemically synthesized for sensory evalu ...
peptides, etc ; Paralichthys olivaceus; cytoplasm; human chorionic gonadotropin; hybridization; immunohistochemistry; lipid metabolism; neurosecretory system; oocytes; osmoregulation; ovarian development; transcription (genetics); Show all 12 Subjects
Abstract:
... Urotensin II (UII) is a kind of fish somatostatins cyclic peptide, which was originally extracted from the caudal neurosecretory system (CNSS). The system of UII and UII receptor (UIIR) has been reported to have multiple physiological regulatory functions, such as cardiovascular control, osmoregulation, and lipid metabolism. However, the effect of UII and UIIR on the ovarian development has not be ...
peptides, etc ; Saccharina japonica; enzymes; food science; gypsum; humans; hydrolysates; macroalgae; medicine; microstructure; sensory evaluation; temperature; texture; tofu; traditional foods; Show all 15 Subjects
Abstract:
... Laminaria japonica Aresch as an edible and medicine dual-purpose marine algae, has been gradually accepted by people. Tofu was Chinese traditional food, combining kelp function ingredients with tofu has practical value. The extraction-optimization methodology and the sequences of kelp antihypertensive peptide (KAP) were investigated. The sensory, whiteness, water retention ability (WRA) and micros ...
peptides, etc ; Nauphoeta cinerea; biomedical research; brain; glucose; hyperglycemia; insects; metabolism; non-specific serine/threonine protein kinase; ribosomal proteins; sequence analysis; streptozotocin; transcription (genetics); Show all 13 Subjects
Abstract:
... The use of insects to model molecular events that characterize degenerative conditions was originally met with scepticism. However, the discovery of insect insulin-like peptides in the 1970's and the demonstration of evolutionary conservation of insulin-related signalling from insects to mammals have highlighted the importance and reduced cost of insect models in biomedical research. Here, we expa ...
Jean Henrottin; Rosa Pilolli; Anne-Catherine Huet; Christof van Poucke; Chiara Nitride; Marc De Loose; Olivier Tranquet; Colette Larré; Karine Adel-Patient; Hervé Bernard; E.N. Clare Mills; Nathalie Gillard; Linda Monaci
... Developing reliable methodologies for detecting and quantifying allergens in processed food commodities is crucial to support food business operators in allergen risk assessment and properly implementing precautionary allergen labels whenever required to safeguard the health of allergic consumers. Multiple Mass Spectrometry (MS) methods have been developed so far and applied for single and multi-a ...
antimicrobial peptides; cyclic peptides, etc ; amino acids; biocompatibility; yeasts; Show all 5 Subjects
Abstract:
... As host defense peptides, peptide antibiotics exist in almost all organisms. Many of their activities come from their inactivation of bacteria, yeast, fungi, and even cancer cells. However, natural peptide antibiotics are relatively poor in stability and penetration, and have high hemolytic properties, which makes them difficult to directly apply. Therefore, natural peptide antibiotics can be modi ...
antimicrobial peptides; synthetic peptides, etc ; amino acids; biotechnology; computer simulation; drugs; evolution; labor; Show all 8 Subjects
Abstract:
... As we learn more about how peptide structure and activity are related, we anticipate that antimicrobial peptides will be engineered to have strong potency and distinct functions and that synthetic peptides will have new biomedical applications, such as treatments for emerging infectious diseases. As a result of the enormous number of possible amino acid sequences and the low-throughput nature of a ...
... In peptide synthesis, it is important to distinguish the terminal amino group and carry out the selective transformation of only the N-terminal protecting group. We describe herein a reaction for the chemo- and site-selective replacement of carbamates with various other carbamates only at the N-terminus of peptides. We demonstrate the scope of carbamates and peptides and the introduction of fluori ...
... Herein, we describe an approach for the on-demand disassembly of dimeric peptides using a palladium-mediated cleavage of a designed self-immolative linker. The utility of the strategy is demonstrated for the case of dimeric basic regions of bZIP transcription factors. While the dimer binds designed DNA sequences with good affinities, the peptide–DNA complex can be readily dismounted by addition of ...
... Intramolecular S-acylation of a thiol-installed threonine with a thioester unit, followed by S–O acyl transfer and subsequent desulphurisation, allows the synthesis of lactone peptides. A protocol has been developed enabling the cyclisation of a linear peptide, a reaction which has not been achieved by conventional methods. ...
... Constrained peptides have proven to be a rich source of ligands for protein surfaces, but are often limited in their binding potency. Deployment of nonnatural side chains that access unoccupied crevices on the receptor surface offers a potential avenue to enhance binding affinity. We recently described a computational approach to create topographic maps of protein surfaces to guide the design of n ...
... Forging new C(sp³)–C(sp³) bonds to central positions within a peptide backbone is critical for the development of new therapeutics and chemical probes. Currently, there are no methods for decarboxylating Asp and Glu side chains solid-phase photochemically or using such radicals to form peptide macrocycles. Herein, electron-donor-acceptor complexes between Hantzsch ester and on-resin peptide N-hydr ...
... Considering that the pH in the tumor microenvironment is dysregulated, we designed a β-hairpin peptide (SSRFEWEFESSᴰPRGDPSSRFEWEFESS). The configuration of the peptide switched from a flexible linear to a rigid loop structure under weakly acidic conditions. The peptide internalized by tumor cells increased significantly under weakly acidic conditions. ...
... Simulating the process of amyloid aggregation with atomic detail is a challenging task for various reasons. One of them is that it is difficult to parametrise a force field such that all protein states ranging from the folded through the unfolded to the aggregated state are represented with the same level of accuracy. Here, we test whether the consideration of electronic polarisability improves th ...
... Pairs of peptide amphiphiles with immiscible hydrophobic tails were synthesized and their assembly formation was investigated. These pairs formed self-sorting supramolecular fibres using a standard heating–cooling protocol, while one pair with longer hydrophobic tails formed a co-assembly when an additional heating process was applied. ...
... The assembly of complexes following the detection of extracellular signals is often controlled by signaling proteins comprising multiple peptide binding modules. The SRC homology (SH)3 family represents the archetypical modular protein interaction module, with ~300 annotated SH3 domains in humans that regulate an impressive array of signaling processes. We review recent findings regarding the allo ...
... The ¹⁸F labeling of unprotected peptides and sugars with a Au(III)–[¹⁸F]fluoroaryl complex is reported. The chemoselective method generates ¹⁸F-labeled S-aryl bioconjugates in an aqueous environment in 15 min with high radiochemical yields and displays excellent functional group tolerance. This approach utilizes an air and moisture stable, robust organometallic Au(III) complex and highlights the v ...
... Peptide modification by C(sp³)–H functionalization of internal residues remains a major challenge due to the inhibitory effect of peptide bonds. In this work, we developed a methionine-directed β-C(sp³)–H arylation method for internal alanine functionalization. By tuning the σC–C bond rotation of internal Ala through head-to-tail cyclization, we overcame the inhibitory effect and functionalized a ...
... The NMR experiment design strategy of NO Relaxation Delay (NORD), introduced mostly as an idealized theoretical approach, is extended and put to practical use by considering synergy and sensitivity-balance in concatenation of experiments. It is illustrated by a novel experiment, NORD {HMBC}–{HSQC}–{TOCSY}, where magnetization of non-¹³C attached protons effectively is channeled from the TOCSY spec ...
... NIR etchable nanoparticles were prepared from NIR-triggered molecule (ASBBCM) and short anticancer peptide segment. After NIR irradiation, the surface of nanoparticles became rough according to the TEM observation due to ASBBCM degradation. Since the nanoparticles were gradually destroyed from the surface, functional YSV peptides were released simultaneously. The release rates were closely related ...