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phosphorylation, etc ; DNA; Western blotting; apoptosis; autophagy; flame retardants; flow cytometry; humans; neurons; neurotoxicity; phosphates; protective effect; rats; China; Show all 14 Subjects
Abstract:
... Tris (1,3-dichloro-2-propyl) phosphate (TDCIPP) is a commonly used organophosphate-based flame retardant and can bio-accumulate in human tissues and organs. As its structure is similar to that of neurotoxic organophosphate pesticides, the neurotoxicity of TDCIPP has raised widespread concerns. TDCIPP can increase neuronal apoptosis and induce autophagy. However, its regulatory mechanism remains un ...
phosphorylation, etc ; aflatoxin M1; body weight; environment; human health; immunosuppression; immunotoxicity; intestines; mice; microRNA; milk; ochratoxin A; risk; transcriptome; transcriptomics; Show all 15 Subjects
Abstract:
... Aflatoxin M1 (AFM1) and ochratoxin A (OTA) are common mycotoxins in cereal foods and milk products, and may cause serious negative impacts on human health. The intestine is crucial for immune regulation as it protects host homeostatic health from external contaminants; however, the underlying mechanisms of AFM1 and OTA mediated intestinal immunotoxicity remain unclear. In this study, whole transcr ...
... Yellow cultivars of Flammulina filiformis are prone to browning, which causes significant yearly loss. This study investigated the critical factors involved and revealed that oxygen plays an essential role in the browning process. Integrated multi-omic analyses, including genome sequencing, transcriptome profiling, and 4D label-free proteome quantification, were incorporated to dissect essential g ...
... Flavonoids are associated with health benefits, but most of them have poor oral bioavailability due to their extremely low aqueous solubility. Flavonoid O-phosphorylation suggests a potent modification to solve the problems. Here, we isolated, identified and characterized an unprecedented phosphotransferase, flavonoid phosphate synthetase (BsFPS), from B. subtilis. The enzyme catalyzes the ATP-dep ...
... The biological effects of the pesticide and mitochondrial complex I inhibitor tebufenpyrad (TEBU) on liver cells were investigated by combining proteomics and metabolomics. Both cell culture media and cellular lysates were analyzed in dose-response and kinetic experiments on the HepaRG cell line. Responses were compared with those obtained on primary human and rat hepatocytes. A multitude of phase ...
... Traditional Chinese preserved egg products have exhibited some anti-inflammatory effects, but their mechanisms of action remain unknown. This study aimed to investigate the anti-inflammatory effects of preserved egg white (PEW) treatment on dextran sulfate sodium (DSS)-induced colitis in mice and the underlying mechanisms. The results showed that treatment with PEW in mice with DSS-induced colitis ...
... In traditional Chinese medicine (TCM) theory, depression is an emotional disease, which is thought to be related to stagnation of liver qi and dysfunction of the spleen in transport. Xiaoyao San (XYS) is considered to have the effects of soothing liver-qi stagnation and invigorating the spleen. The spleen has the function to transport and transform nutrients. The liver has also termed the center o ...
phosphorylation, etc ; Actaea cimicifuga; Oriental traditional medicine; Western blotting; blood serum; cytotoxicity; edema; enzyme-linked immunosorbent assay; eosin; fluorescent antibody technique; genes; glycosides; histopathology; interleukin-6; lipopolysaccharides; lung function; lungs; macrophages; models; nitric oxide; pharyngitis; plethysmography; protein content; rhizomes; therapeutics; toxicity testing; triterpenoids; Show all 27 Subjects
Abstract:
... Cimicifuga foetida L. is a well-established traditional Chinese medicine with heat-clearing and detoxifying effects and has good therapeutic effect on oral mucosal ulcer and pharyngitis. The rhizome of this herb is rich in triterpenoid glycosides, including 23-O-acetylshengmanol-3-o-α-L-arabinoside (DA). Whether and how DA attenuates acute lung injury (ALI) are unclear. Accordingly, we focused on ...
... A short-term study was performed to assess the effects of diets supplemented with polyphenols (CSVP), extracted from the chestnut (Castanea sativa) shell, agri-food waste rich in tannins, and mullein (Verbascum macrurum), a perennial spontaneous plant rich in flavonoids, on a zebrafish (Danio rerio) model of k-carrageenan-induced intestinal inflammation. Morphological asset of intestine, immunoden ...
phosphorylation, etc ; adenosinetriphosphatase; mammals; Show all 3 Subjects
Abstract:
... Despite a long appreciation for the role of nonsense-mediated mRNA decay (NMD) in destroying faulty, disease-causing mRNAs and maintaining normal, physiologic mRNA abundance, additional effectors that regulate NMD activity in mammalian cells continue to be identified. Here, we describe a haploid-cell genetic screen for NMD effectors that has unexpectedly identified 13 proteins constituting the AKT ...
phosphorylation, etc ; phosphates; secondary metabolites; Show all 3 Subjects
Abstract:
... The phosphate ester is a versatile, widespread functional group involved in a plethora of biological activities. Its presence in secondary metabolites, however, is relatively rare compared to other functionalities and thus is part of a rather unexplored chemical space. Herein, the chemistry of secondary metabolites containing the phosphate ester group is discussed. The text emphasizes their struct ...
Ioannis Sanidas; Hanjun Lee; Purva H. Rumde; Gaylor Boulay; Robert Morris; Gabriel Golczer; Marcelo Stanzione; Soroush Hajizadeh; Jun Zhong; Meagan B. Ryan; Ryan B. Corcoran; Benjamin J. Drapkin; Miguel N. Rivera; Nicholas J. Dyson; Michael S. Lawrence
... The interaction of RB with chromatin is key to understanding its molecular functions. Here, for first time, we identify the full spectrum of chromatin-bound RB. Rather than exclusively binding promoters, as is often described, RB targets three fundamentally different types of loci (promoters, enhancers, and insulators), which are largely distinguishable by the mutually exclusive presence of E2F1, ...
Wesley B. Asher; Daniel S. Terry; G. Glenn A. Gregorio; Alem W. Kahsai; Alessandro Borgia; Bing Xie; Arnab Modak; Ying Zhu; Wonjo Jang; Alekhya Govindaraju; Li-Yin Huang; Asuka Inoue; Nevin A. Lambert; Vsevolod V. Gurevich; Lei Shi; Robert J. Lefkowitz; Scott C. Blanchard; Jonathan A. Javitch
phosphorylation, etc ; energy transfer; fluorescence; phosphopeptides; Show all 4 Subjects
Abstract:
... β-arrestins bind G protein-coupled receptors to terminate G protein signaling and to facilitate other downstream signaling pathways. Using single-molecule fluorescence resonance energy transfer imaging, we show that β-arrestin is strongly autoinhibited in its basal state. Its engagement with a phosphopeptide mimicking phosphorylated receptor tail efficiently releases the β-arrestin tail from its N ...
phosphorylation, etc ; glycerol; organic chemistry; oxidation; Show all 4 Subjects
Abstract:
... Recent years have witnessed significant achievements in the field of organic chemistry, which have led to new drugs and the discovery of new and biologically interesting molecules. Herein, we describe a practical and efficient approach to the synthesis of enantiomerically pure and diverse lysobisphosphatidic acid analogues. The key feature of the synthesis is a one-pot, sequential phosphorylation ...
Juan Luis Pacheco‐Garcia; Dmitry Loginov; Bruno Rizzuti; Pavla Vankova; Jose L. Neira; Daniel Kavan; Noel Mesa‐Torres; Rita Guzzi; Petr Man; Angel L. Pey
phosphorylation, etc ; bioinformatics; humans; mutation; rats; Show all 5 Subjects
Abstract:
... The phosphomimetic mutation S82D in the cancer‐associated, FAD‐dependent human NADP(H):quinone oxidoreductase 1 (hNQO1) causes a decrease in flavin‐adenine dinucleotide‐binding affinity and intracellular stability. We test in this work whether the evolutionarily recent neutral mutation R80H in the vicinity of S82 may alter the strong functional effects of S82 phosphorylation through electrostatic ...
phosphorylation, etc ; hydrogen; neoplasms; organic chemistry; Show all 4 Subjects
Abstract:
... An efficient copper-catalyzed aerobic oxidative cross-dehydrogenative coupling reaction for the synthesis of multisubstituted phosphorylhydrazides from N,N-disubstituted hydrazines and hydrogen phosphoryl compounds is accomplished. The reaction proceeds under mild conditions without the addition of any external oxidants and bases. This work reported here represents a direct P(═O)–N–N bond formatio ...
phosphorylation, etc ; Drosophila; dimerization; homeostasis; humans; Show all 5 Subjects
Abstract:
... Hippo signalling plays key role in tissue growth and homeostasis, and its dysregulation is implicated in various human diseases. Expanded (Ex) is an important upstream activator of Hippo signalling; however, how Ex activates Hippo signalling is still poorly understood. Here, we demonstrate that Ex forms a homodimer via C‐terminal interaction, and that the ExC2 region (912–1164 aa) is sufficient an ...
... The DNA-dependent protein kinase (DNA-PK) initially protects broken DNA ends but then promotes their processing during non-homologous end joining (NHEJ). Before ligation by NHEJ, DNA hairpin ends generated during V(D)J recombination must be opened by the Artemis nuclease, together with autophosphorylated DNA-PK. Structures of DNA-PK bound to DNA before and after phosphorylation, and in complex wit ...
phosphorylation, etc ; adults; homeostasis; neurotransmitters; tissue repair; Show all 5 Subjects
Abstract:
... The normal functioning of the adult organism is based on the processes of maintaining homeostasis, tissue repair, and regeneration. An important role in the regulation of these processes is played by a special group of postnatal stem cells: multipotent mesenchymal stromal cells (MSCs). The functional activity of MSCs is under strict neuroendocrine control. In this review, the features of the mecha ...
phosphorylation, etc ; antagonists; luteolysis; progesterone; renin; secretion; Show all 6 Subjects
Abstract:
... The aim of this study was to evaluate the role of prorenin/(pro)renin receptor activation on luteal progesterone (P4) secretion. Our hypothesis was that the nonproteolytic activation of (pro)renin receptor [P(RR)] is part of the regulatory mechanism responsible for corpus luteum (CL) function. In the first three experiments, prorenin was found to stimulate the production of P4, which is not inhibi ...