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1. Tools for Understanding Nanoscale Lipid Regulation of Ion Channels

Author:
Robinson, Carol V.; Rohacs, Tibor; Hansen, Scott B.
Source:
Trends in biochemical sciences 2019
ISSN:
0968-0004
Subject:
plasma membrane,   etc   ; ion channels; phospholipids;     Show all 3 Subjects
Abstract:
... Anionic phospholipids are minor but prominent components of the plasma membrane that are necessary for ion channel function. Their persistence in bulk membranes, in particular phosphatidylinositol 4,5-bisphosphate (PIP2), initially suggested they act as channel cofactors. However, recent technologies have established an emerging system of nanoscale signaling to ion channels based on lipid compartm ...
DOI:
10.1016/j.tibs.2019.04.001

2. GPCR interaction as a possible way for allosteric control between receptors

Author:
Pin, Jean-Philippe; Kniazeff, Julie; Prézeau, Laurent; Liu, Jiang-Feng; Rondard, Philippe
Source:
Molecular and cellular endocrinology 2019
ISSN:
0303-7207
Subject:
plasma membrane,   etc   ; G-protein coupled receptors; models;     Show all 3 Subjects
Abstract:
... For more than twenty years now, GPCR dimers and larger oligomers have been the subject of intense debates. Evidence for a role of such complexes in receptor trafficking to and from the plasma membrane have been provided. However, one main issue is of course to determine whether or not such a phenomenon can be responsible for an allosteric and reciprocal control (allosteric control) of the subunits ...
DOI:
10.1016/j.mce.2019.02.019

3. ORP2 Delivers Cholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4, 5-Bisphosphate (PI(4,5)P2)

Author:
Wang, Huan; Ma, Qianli; Qi, Yanfei; Dong, Jiangqing; Du, Ximing; Rae, James; Wang, Jue; Wu, Wei-Feng; Brown, Andrew J.; Parton, Robert G.; Wu, Jia-Wei; Yang, Hongyuan
Source:
Molecular cell 2019 v.73 no.3 pp. 458-473.e7
ISSN:
1097-2765
Subject:
plasma membrane,   etc   ; lipid transfer proteins; oxysterols;     Show all 3 Subjects
Abstract:
... Cholesterol is highly enriched at the plasma membrane (PM), and lipid transfer proteins may deliver cholesterol to the PM in a nonvesicular manner. Here, through a mini-screen, we identified the oxysterol binding protein (OSBP)-related protein 2 (ORP2) as a novel mediator of selective cholesterol delivery to the PM. Interestingly, ORP2-mediated enrichment of PM cholesterol was coupled with the rem ...
DOI:
10.1016/j.molcel.2018.11.014

4. Sperm quality during extra‐gonadal sperm reserve depletion in stallions

Author:
Len, Jose A.; Beehan, David; Eilts, Bruce; Lyle, Sara K.
Source:
Reproduction in domestic animals 2019 v.54 no.2 pp. 412-414
ISSN:
0936-6768
Subject:
plasma membrane,   etc   ; sperm motility; sperm quality; stallions;     Show all 4 Subjects
Abstract:
... To estimate when, during stallions’ extra‐gonadal reserves (EGR) depletion period, sperm quality would reach its highest quality, six light breed sexually rested stallions were collected daily for 7 days to deplete EGR. On collection days 1, 3, 5, and 7, sperm output, total (TM) and progressive (PM) motility, morphology, and plasma membrane (PLM) integrity were evaluated. Sperm output decreased as ...
DOI:
10.1111/rda.13359

5. Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched

Author:
Zhang, Yunxiao; Bulkley, David P.; Xin, Yao; Roberts, Kelsey J.; Asarnow, Daniel E.; Sharma, Ashutosh; Myers, Benjamin R.; Cho, Wonhwa; Cheng, Yifan; Beachy, Philip A.
Source:
Cell 2018 v.175 no.5 pp. 1352-1364.e14
ISSN:
0092-8674
Subject:
plasma membrane,   etc   ; cholesterol; hydrophobicity; transporters;     Show all 4 Subjects
Abstract:
... Hedgehog protein signals mediate tissue patterning and maintenance by binding to and inactivating their common receptor Patched, a 12-transmembrane protein that otherwise would suppress the activity of the 7-transmembrane protein Smoothened. Loss of Patched function, the most common cause of basal cell carcinoma, permits unregulated activation of Smoothened and of the Hedgehog pathway. A cryo-EM s ...
DOI:
10.1016/j.cell.2018.10.026

6. Divide and Rule: Plant Plasma Membrane Organization

Author:
Gronnier, Julien; Gerbeau-Pissot, Patricia; Germain, Véronique; Mongrand, Sébastien; Simon-Plas, Françoise
Source:
Trends in plant science 2018 v.23 no.10 pp. 899-917
ISSN:
1360-1385
Subject:
plasma membrane,   etc   ; models; signal transduction;     Show all 3 Subjects
Abstract:
... Since the publication of the fluid mosaic as a relevant model for biological membranes, accumulating evidence has revealed the outstanding complexity of the composition and organization of the plant plasma membrane (PM). Powerful new methodologies have uncovered the remarkable multiscale and multicomponent heterogeneity of PM subcompartmentalization, and this is emerging as a general trait with di ...
DOI:
10.1016/j.tplants.2018.07.007

7. Enhancement of drug electrotransfer by extracellular plasmid DNA

Author:
Ruzgys, Paulius; Jakutaviciute, Milda; Chopra, Sonam; Satkauskas, Saulius
Source:
Archives of biochemistry and biophysics 2019 v.666 pp. 156-160
ISSN:
0003-9861
Subject:
plasma membrane,   etc   ; drugs; electric field; electroporation; fluorescent dyes; plasmids; transfection;     Show all 7 Subjects
Abstract:
... Electroporation is a widely established method for molecular delivery across electric field perturbed plasma membrane. It can be used as a non-viral DNA transfection method, or as a way to achieve small molecule delivery to or extraction from cells. We examined the possibility of combining the DNA delivery to the cells with small molecule transport across electroporated plasma membrane. The result ...
DOI:
10.1016/j.abb.2018.12.010

8. Extracting lipid vesicles from plasma membranes via self-assembly of clathrin-inspired scaffolding nanoparticles

Author:
Li, Ye; Zhang, Xianren; Lin, Jinxing; Li, Ruili; Yue, Tongtao
Source:
Colloids and surfaces 2019 v.176 pp. 239-248
ISSN:
0927-7765
Subject:
plasma membrane,   etc   ; clathrin; colloids; endocytosis; guidelines; lipids; nanoparticles; surface tension;     Show all 8 Subjects
Abstract:
... Single-cell analysis is a new and rapidly expanding field, the goal of which is obtaining fresh information from individual cells to understand the regulatory mechanisms of cell development and diseases. Conventional approaches generally rely on the cell lysis which, however, is destructive to cells and against multiple sampling from the living cell. Here, we propose and design a scaffolding nanop ...
DOI:
10.1016/j.colsurfb.2019.01.008

9. The auxin influx carrier, OsAUX3, regulates rice root development and responses to aluminium stress

Author:
Wang, Mei; Qiao, JiYue; Yu, ChenLiang; Chen, Hao; Sun, ChenDong; Huang, LinZhou; Li, ChuanYou; Geisler, Markus; Qian, Qian; Jiang, De An; Qi, YanHua
Source:
Plant, cell and environment 2019 v.42 no.4 pp. 1125-1138
ISSN:
0140-7791
Subject:
plasma membrane,   etc   ; aluminum; naphthaleneacetic acid; oxygen; rice; root hairs;     Show all 6 Subjects
Abstract:
... In rice, there are five members of the auxin carrier AUXIN1/LIKE AUX1 family; however, the biological functions of the other four members besides OsAUX1 remain unknown. Here, by using CRISPR/Cas9, we constructed two independent OsAUX3 knock‐down lines, osaux3‐1 and osaux3‐2, in wild‐type rice, Hwayoung (WT/HY) and Dongjin (WT/DJ). osaux3‐1 and osaux3‐2 have shorter primary roots (PRs), decreased l ...
DOI:
10.1111/pce.13478

10. Cooperativity in proton sensing by PIP aquaporins

Author:
Vitali, Victoria; Jozefkowicz, Cintia; Canessa Fortuna, Agustina; Soto, Gabriela; González Flecha, F. Luis; Alleva, Karina
Source:
TheFEBS journal 2019 v.286 no.5 pp. 991-1002
ISSN:
1742-464X
Subject:
plasma membrane,   etc   ; aquaporins; binding sites; models; pH; physiological transport; protons;     Show all 7 Subjects
Abstract:
... One of the most intriguing properties of plasma membrane intrinsic protein (PIP) aquaporins (AQPs) is their ability to modulate water transport by sensing different levels of intracellular pH through the assembly of homo‐ and heterotetrameric molecular species in the plasma membrane. In this work, using a phenomenological modeling approach, we demonstrate that cooperativity in PIP biological respo ...
DOI:
10.1111/febs.14701

11. Molecular Discrimination between Two Conformations of Sphingomyelin in Plasma Membranes

Author:
Endapally, Shreya; Frias, Donna; Grzemska, Magdalena; Gay, Austin; Tomchick, Diana R.; Radhakrishnan, Arun
Source:
Cell 2019 v.176 no.5 pp. 1040-1053.e17
ISSN:
0092-8674
Subject:
plasma membrane,   etc   ; cholesterol; heat production; point mutation; sphingomyelins;     Show all 5 Subjects
Abstract:
... Sphingomyelin and cholesterol are essential lipids that are enriched in plasma membranes of animal cells, where they interact to regulate membrane properties and many intracellular signaling processes. Despite intense study, the interaction between these lipids in membranes is not well understood. Here, structural and biochemical analyses of ostreolysin A (OlyA), a protein that binds to membranes ...
DOI:
10.1016/j.cell.2018.12.042

12. Binding kinetics of ligands acting at GPCRs

Author:
Sykes, David A.; Stoddart, Leigh A.; Kilpatrick, Laura E.; Hill, Stephen J.
Source:
Molecular and cellular endocrinology 2019
ISSN:
0303-7207
Subject:
plasma membrane,   etc   ; G-protein coupled receptors; dissociation; equations; ligands; therapeutics;     Show all 6 Subjects
Abstract:
... The influence of drug-receptor binding kinetics has often been overlooked during the development of new therapeutics that target G protein-coupled receptors (GPCRs). Over the last decade there has been a growing understanding that an in-depth knowledge of binding kinetics at GPCRs is required to successfully target this class of proteins. Ligand binding to a GPCR is often not a simple single step ...
DOI:
10.1016/j.mce.2019.01.018

13. Plant cell surface immune receptor complex signaling

Author:
Wan, Wei-Lin; Katja, Fröhlich; Pruitt, Rory N; Thorsten, Nürnberger; Zhang, Lisha
Source:
Current opinion in plant biology 2019
ISSN:
1369-5266
Subject:
plasma membrane,   etc   ; crops; immune response; immunologic receptors; protein kinases;     Show all 5 Subjects
Abstract:
... Plant plasma membrane pattern recognition receptors are key to microbe sensing and activation of immunity to microbial invasion. Plants employ several types of such receptors that differ mainly in the structure of their ectodomains and the presence or absence of a cytoplasmic protein kinase domain. Plant immune receptors do not function as single entities, but form larger complexes which undergo c ...
DOI:
10.1016/j.pbi.2019.02.001

14. Codetermination of Sphingomyelin and Cholesterol in Cellular Plasma Membrane in Sphingomyelin-Depletion-Induced Cholesterol Efflux

Author:
Huang, Shuohan; Liu, Kang; Jiang, Dechen; Fang, Danjun
Source:
Analytical chemistry 2018 v.91 no.2 pp. 1501-1506
ISSN:
1520-6882
Subject:
plasma membrane,   etc   ; ABC transporters; cholesterol; electrochemiluminescence; sphingomyelins;     Show all 5 Subjects
Abstract:
... Quantification of multiple lipids with different contents in plasma membrane in single cells is significant, but challenging, for investigating lipid interactions and the role of dominant protein transporters. In this paper, comonitoring the alteration of low-content sphingomyelin (SM) and high-content cholesterol in plasma membrane of one living cell is realized by use of luminol electrochemilumi ...
DOI:
10.1021/acs.analchem.8b04593

15. Geminivirus C4: Interplaying with Receptor-like Kinases

Author:
Zeng, Runxiu; Liu, Xiaoshi; Yang, Chengwei; Lai, Jianbin
Source:
Trends in plant science 2018 v.23 no.12 pp. 1044-1046
ISSN:
1360-1385
Subject:
plasma membrane,   etc   ; Geminiviridae; phosphotransferases (kinases); proteins; viruses;     Show all 5 Subjects
Abstract:
... Geminivirus infection results in systemic symptoms in plants, but the mechanism by which the virus affects cell-to-cell signalling involved in development and resistance remains unclear. Based on recent evidence, we suggest a potential mechanism that geminivirus C4 proteins interfere with intercellular signals by interacting with receptor-like kinases on the plasma membrane. ...
DOI:
10.1016/j.tplants.2018.09.003

16. The VirE-asy Way to Genetically Transform Plants

Author:
Gelvin, Stanton B.
Source:
Trends in microbiology 2018 v.26 no.12 pp. 973-975
ISSN:
0966-842X
Subject:
plasma membrane,   etc   ; Agrobacterium; proteins; transfer DNA; virulence;     Show all 5 Subjects
Abstract:
... Agrobacterium transfers T-DNA and several virulence effector proteins to plant cells. It is not known how and where T-complexes containing these components are assembled. A new study suggests that T-complexes form on the plant plasma membrane, mediated by the effector protein VirE3. ...
DOI:
10.1016/j.tim.2018.10.003

17. Liposomes and polymersomes: a comparative review towards cell mimicking

Author:
Rideau, Emeline; Dimova, Rumiana; Schwille, Petra; Wurm, Frederik R.; Landfester, Katharina
Source:
Chemical Society reviews 2018 v.47 no.23 pp. 8572-8610
ISSN:
1460-4744
Subject:
plasma membrane,   etc   ; biomimetics; models; synthetic biology;     Show all 4 Subjects
Abstract:
... Cells are integral to all forms of life due to their compartmentalization by the plasma membrane. However, living organisms are immensely complex. Thus there is a need for simplified and controllable models of life for a deeper understanding of fundamental biological processes and man-made applications. This is where the bottom-up approach of synthetic biology comes from: a stepwise assembly of bi ...
DOI:
10.1039/c8cs00162f

18. Plant cell surface molecular cypher: Receptor-like proteins and their roles in immunity and development

Author:
Jamieson, Pierce A.; Shan, Libo; He, Ping
Source:
Plant science 2018 v.274 pp. 242-251
ISSN:
0168-9452
Subject:
plasma membrane,   etc   ; immunity; phosphotransferases (kinases); receptors;     Show all 4 Subjects
Abstract:
... Plant receptor-like proteins (RLPs) are a family of transmembrane receptors which are distinguished from receptor-like kinases (RLKs) by their lack of a cytoplasmic kinase domain. RLPs continue to be implicated in a broad range of plant immunological and developmental processes as critical sensors or participants in receptor complexes on the plasma membrane. RLPs often associate with RLKs to activ ...
DOI:
10.1016/j.plantsci.2018.05.030

19. Synthetic Strategies for Modified Glycosphingolipids and Their Design as Probes

Author:
Hunter, Carmanah D.; Guo, Tianlin; Daskhan, Gour; Richards, Michele R.; Cairo, Christopher W.
Source:
Chemical reviews 2018 v.118 no.17 pp. 8188-8241
ISSN:
1520-6890
Subject:
plasma membrane,   etc   ; fluorescent dyes; glycosphingolipids; vertebrates;     Show all 4 Subjects
Abstract:
... The plasma membrane of cells contains a diverse array of lipids that provide important structural and biological features. Glycolipids are typically a minor component of the cell membrane and consist primarily of glycosphingolipids (GSLs). GSLs in vertebrates contain a multifarious assortment of glycan headgroups, which can be important to biological functions based on lipid–lipid and lipid–protei ...
DOI:
10.1021/acs.chemrev.8b00070

20. Evidence of G-protein-coupled receptor and substrate transporter heteromerization at a single molecule level

Author:
Fischer, Jana; Kleinau, Gunnar; Rutz, Claudia; Zwanziger, Denise; Khajavi, Noushafarin; Müller, Anne; Rehders, Maren; Brix, Klaudia; Worth, CatherineL.; Führer, Dagmar; Krude, Heiko; Wiesner, Burkhard; Schülein, Ralf; Biebermann, Heike
Source:
Cellular and molecular life sciences 2018 v.75 no.12 pp. 2227-2239
ISSN:
1420-682X
Subject:
plasma membrane,   etc   ; spectroscopy; thyroid hormones; thyrotropin receptors;     Show all 4 Subjects
Abstract:
... G-protein-coupled receptors (GPCRs) can constitute complexes with non-GPCR integral membrane proteins, while such interaction has not been demonstrated at a single molecule level so far. We here investigated the potential interaction between the thyrotropin receptor (TSHR) and the monocarboxylate transporter 8 (MCT8), a member of the major facilitator superfamily (MFS), using fluorescence cross-co ...
DOI:
10.1007/s00018-017-2728-1