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1. A modified method for plasmid extraction from Lactobacillus plantarum contained lysozyme removal step

Author:
Yao, F.; Xu, X.Y.; Pan, Q.
Source:
Analytical biochemistry 2019 v.566 pp. 37-39
ISSN:
0003-2697
Subject:
plasmids,   etc   ; Lactobacillus plantarum; Weissella cibaria; lysozyme; protocols;     Show all 5 Subjects
Abstract:
... Plasmids of Lactobacillus plantarum PC518 cannot be effectively extracted by existing methods. It was studied that the effect of lysozyme treatment and removal on plasmid extraction by 7 protocols. The modified method was compared with a commercial kit using L. plantarum PC518, 410, 9L15, and JS193 and Weissella cibaria M2 as the tested strains. The results suggested that the step of lysozyme remo ...
DOI:
10.1016/j.ab.2018.11.005

2. Correction to: Rational design of engineered microbial cell surface multi-enzyme co-display system for sustainable NADH regeneration from low-cost biomass

Author:
Han, Lei; Liang, Bo; Song, Jianxia; Liu, Aihua
Source:
Journal of industrial microbiology & biotechnology 2018 v.45 no.9 pp. 855
ISSN:
1367-5435
Subject:
plasmids,   etc   ; NAD (coenzyme); biomass;     Show all 3 Subjects
Abstract:
... In the original publication, the author group has been published incorrectly. All authors have agreed to add Aihua Liu as co-author of this article, since the original idea for the experiments, the experimental expertise and some of the plasmids originated from him. ...
DOI:
10.1007/s10295-018-2065-x

3. Biodegradation of dinitrotoluene sulfonates and other nitro-aromatic compounds by Pseudomonas sp. X5 isolated from TNT red water contaminated soil

Author:
Xu, Wenjie; Zhao, Quanlin; Li, Zhilin; Lu, Xinyue; Han, Shiqun; Ye, Zhengfang
Source:
Journal of cleaner production 2019 v.214 pp. 782-790
ISSN:
0959-6526
Subject:
plasmid curing; plasmids,   etc   ; Pseudomonas; bacteria; biodegradation; genes; liquid chromatography; mass spectrometry; nitroaromatic compounds; pH; pollutants; polluted soils; salinity; sulfonates; trinitrotoluene; water pollution; China;     Show all 17 Subjects
Abstract:
... A dinitrotoluene sulfonates degrading bacterium strain Pseudomonas sp. X5 was isolated from 2, 4, 6-trinitrotoluene red water contaminated soil in Gansu, China, which was used for biodegradation of dinitrotoluene sulfonates. The growth experiments were carried out to optimize the growth condition of X5. Effect of inoculation amount was investigated. Plasmid curing tests were conducted to determine ...
DOI:
10.1016/j.jclepro.2019.01.025

4. Oxacillin-susceptible mecA-positive Staphylococcus aureus associated with processed food in Europe

Author:
Quijada, Narciso M.; Hernández, Marta; Oniciuc, Elena-Alexandra; Eiros, José María; Fernández-Natal, Isabel; Wagner, Martin; Rodríguez-Lázaro, David
Source:
Food microbiology 2019 v.82 pp. 107-110
ISSN:
0740-0020
Subject:
plasmids,   etc   ; air; airports; cheeses; gene expression; genes; methicillin-resistant Staphylococcus aureus; processed foods; Austria;     Show all 9 Subjects
Abstract:
... We report for the first time an oxacillin-susceptible mecA-positive Staphylococcus aureus (OS-MRSA) associated with a processed food product in Europe. One isolate (MRSA-ST5-type V SCCmec) was found in cheese among 600 food samples confiscated from air passengers from international flights in Vienna Airport (Austria). Type V SCCmec strains do not harbor functional mecI-mecR1 genes and in such stra ...
DOI:
10.1016/j.fm.2019.01.021

5. Redox-responsive polymer inhibits macrophages uptake for effective intracellular gene delivery and enhanced cancer therapy

Author:
Wen, Lijuan; Hu, Yingwen; Meng, Tingting; Tan, Yanan; Zhao, Mengdan; Dai, Suhuan; Yuan, Hong; Hu, Fuqiang
Source:
Colloids and surfaces 2019 v.175 pp. 392-402
ISSN:
0927-7765
Subject:
plasmids,   etc   ; colloids; disulfide bonds; drug delivery systems; gene transfer; genes; glutathione; macrophages; neoplasm cells; neoplasms; polymers;     Show all 11 Subjects
Abstract:
... The development of advanced gene delivery carriers with stimuli-responsive release manner for tumor therapeutics is desirable, since they can exclusively release the therapeutic gene via their structural changes in response to the specific stimuli of the target site. Moreover, interactions between macrophages and drug delivery systems (DDSs) seriously impair the treatment efficiency of DDSs, thus ...
DOI:
10.1016/j.colsurfb.2018.12.016

6. A plant intron enhances the performance of an infectious clone in planta

Author:
Tran, Phu-Tri; Fang, Miao; Widyasari, Kristin; Kim, Kook-Hyung
Source:
Journal of virological methods 2019 v.265 pp. 26-34
ISSN:
0166-0934
Subject:
plasmids,   etc   ; Agrobacterium; Escherichia coli; bacteria; clones; enzymes; introns; leaves; pathogenicity; plant pathology; virology;     Show all 11 Subjects
Abstract:
... Although infectious clones are fundamental tools in virology and plant pathology, their efficacy is often reduced by the instability of viral sequences in Escherichia coli. In this study, we constructed an infectious clone of PepMoV (pPepMoV) in a bacterial binary vector (pSNU1); the clone induces symptoms of PepMoV in agroinfiltrated plants. During its modification and maintenance in E. coli, how ...
DOI:
10.1016/j.jviromet.2018.12.012

7. Universal DNA biosensing based on instantaneously electrostatic attraction between hexaammineruthenium (III) and DNA molecules

Author:
Dai, Siya; Lu, Wei; Wang, Yusheng; Yao, Bo
Source:
Biosensors & bioelectronics 2019 v.127 pp. 101-107
ISSN:
0956-5663
Subject:
plasmids,   etc   ; DNA fragmentation; ammonia; biosensors; electrodes; electrostatic interactions; genomics; gold; nanogold; phosphates; spectrometers;     Show all 11 Subjects
Abstract:
... Despite rapid progress in DNA biosensors by employing various materials as well as techniques, most of the reported sensors are based on specific recognition of a DNA fragment, however can not perform universal measurement of DNA molecules (i.e. genomic DNA). In this work, we proposed a novel DNA biosensing method based on instantaneously electrostatic attraction (IEA) between hexaammineruthenium ...
DOI:
10.1016/j.bios.2018.12.019

8. Graphene-oxide quenching-based molecular beacon imaging of exosome-mediated transfer of neurogenic miR-193a on microfluidic platform

Author:
Oh, Hyun Jeong; Kim, Jaehoon; Park, Hyejin; Chung, Seok; Hwang, Do Won; Lee, Dong Soo
Source:
Biosensors & bioelectronics 2019 v.126 pp. 647-656
ISSN:
0956-5663
Subject:
plasmid vectors,   etc   ; biosensors; exosomes; fluorescence; graphene oxide; image analysis; microRNA; neural stem cells; neurogenesis; neurons; tissues;     Show all 11 Subjects
Abstract:
... Graphene-oxide (GO) quenching-based molecular beacon was developed for rapid and sensitive detection of RNAs in living cells and tissues. Here, we applied GO quenching-based molecular beacon sensor to visualize neurogenic miR-193a levels delivered via exosomes during cell-non-autonomous neurogenesis in neural progenitor cells on a microfluidic platform. Exosomal transport was visualized using CD63 ...
DOI:
10.1016/j.bios.2018.11.027

9. A combination of electrochemotherapy, gene electrotransfer of plasmid encoding canine IL-12 and cytoreductive surgery in the treatment of canine oral malignant melanoma

Author:
Milevoj, Nina; Tratar, Urša Lampreht; Nemec, Ana; Brožič, Andreja; Žnidar, Katarina; Serša, Gregor; Čemažar, Maja; Tozon, Nataša
Source:
Research in veterinary science 2019 v.122 pp. 40-49
ISSN:
0034-5288
Subject:
plasmids,   etc   ; T-lymphocytes; dogs; genes; immune response; interleukin-12; melanoma; patients; protocols; surgery;     Show all 10 Subjects
Abstract:
... The aim of this study was to evaluate the safety and efficacy of the combination of electrochemotherapy (ECT) with bleomycin and gene electrotransfer (GET) of plasmid encoding canine interleukin 12 (IL-12) for the treatment of canine oral malignant melanoma (OMM). Our focus was to determine the effect of the treatment on achieving local tumor control and stimulation of an antitumor immune response ...
DOI:
10.1016/j.rvsc.2018.11.001

10. Engineering of multiple modular pathways for high-yield production of 5-aminolevulinic acid in Escherichia coli

Author:
Zhang, Junli; Weng, Huanjiao; Zhou, Zhengxiong; Du, Guocheng; Kang, Zhen
Source:
Bioresource technology 2019 v.274 pp. 353-360
ISSN:
0960-8524
Subject:
plasmids,   etc   ; Escherichia coli; aminolevulinic acid; biosynthesis; fermenters; metabolic engineering; mutation; pH; pyridoxal; pyridoxal phosphate; transferases;     Show all 11 Subjects
Abstract:
... 5-aminolevulinic acid (ALA), an important precursor of tetrapyrroles, has various applications in medicine and agriculture fields. Several methods have been adopted to enhance ALA synthesis in our previous studies. In this study, systematic metabolic engineering strategies were implemented to further improve ALA production in Escherichia coli. Firstly, hemA and hemL with different strength of RBS ...
DOI:
10.1016/j.biortech.2018.12.004

11. qnrD-harboring plasmids in Providencia spp. recovered from food and environmental Brazilian sources

Author:
Kraychete, Gabriela Bergiante; Campana, Eloiza Helena; Picão, Renata Cristina; Bonelli, Raquel Regina
Source:
The Science of the total environment 2019 v.646 pp. 1290-1292
ISSN:
0048-9697
Subject:
plasmids,   etc   ; Morganella; Proteus; Providencia; Salmonella enterica; coastal water; genes; quinolones; Brazil; China; France;     Show all 11 Subjects
Abstract:
... QnrD is a plasmid-mediated quinolone resistance (PMQR) determinant first reported in clinical Salmonella enterica isolates from China, located on nonconjugative plasmids of 4270 bp. Since then, the qnrD gene has been mostly found on plasmids around 2683 bp in Proteus and Morganella genera. However, Providencia spp. strains carrying qnrD-harboring plasmids have only been reported among clinical sam ...
DOI:
10.1016/j.scitotenv.2018.07.378

12. Transmission routes of ESBL/pAmpC producing bacteria in the broiler production pyramid, a literature review

Author:
Dame-Korevaar, Anita; Fischer, Egil A.J.; van der Goot, Jeanet; Stegeman, Arjan; Mevius, Dik
Source:
Preventive veterinary medicine 2019 v.162 pp. 136-150
ISSN:
0167-5877
Subject:
plasmids,   etc   ; anti-infective agents; bacteria; beta-lactamase; beta-lactams; databases; farms; hatcheries; humans; poultry production;     Show all 10 Subjects
Abstract:
... Plasmid mediated Extended Spectrum Beta-Lactamase and AmpC Beta-Lactamase (ESBL/pAmpC) producing bacteria are resistant to beta-lactam antimicrobials and are widespread in humans, the environment and animals. Animals, especially broilers, are an important reservoir of ESBL/pAmpC producing bacteria. To control ESBL/pAmpC prevalence in broilers, transmission within the entire broiler production pyra ...
DOI:
10.1016/j.prevetmed.2018.12.002

13. Multipartite genomes and the sRNome in response to temperature stress of an Arctic Pseudoalteromonas fuliginea BSW20308

Author:
Liao, Li; Liu, Chun; Zeng, Yinxin; Zhao, Bin; Zhang, Jin; Chen, Bo
Source:
Environmental microbiology 2019 v.21 no.1 pp. 272-285
ISSN:
1462-2912
Subject:
plasmids,   etc   ; Pseudoalteromonas; chromosomes; ecosystems; genome; global warming; heat stress; sequence analysis; temperature; transcriptomics; Arctic region;     Show all 11 Subjects
Abstract:
... Little is known about the survival and effect of rapid climate warming on Pseudoalteromonas in the Arctic, although it is abundant and important in this ecosystem. Here, we investigated a cold‐adapted Pseudoalteromonas fuliginea BSW20308 from the Arctic Ocean, from the genome to its transcriptomic responses towards temperature changes. It contained two circular chromosomes, with the second chromos ...
DOI:
10.1111/1462-2920.14455

14. Extremely halophilic pleomorphic archaeal virus HRPV9 extends the diversity of pleolipoviruses with integrases

Author:
Atanasova, Nina S.; Demina, Tatiana A.; Krishnam Rajan Shanthi, Sudar N.V.; Oksanen, Hanna M.; Bamford, Dennis H.
Source:
Research in microbiology 2018 v.169 no.9 pp. 500-504
ISSN:
0923-2508
Subject:
plasmids,   etc   ; Archaea; enzymes; genes; proviruses; structural proteins;     Show all 6 Subjects
Abstract:
... Certain pleomorphic archaeal viruses are highly infectious even at saturated salt. These viruses belong to the genus Betapleolipovirus of the recently described archaeal virus family Pleolipoviridae. Pleolipoviruses comprise single-stranded or double-stranded, circular or linear DNA genomes that share countless homologues among various archaeal genetic elements. Here we describe a new extremely ha ...
DOI:
10.1016/j.resmic.2018.04.004

15. microRNA-29a inhibition induces Gab1 upregulation to protect OB-6 human osteoblasts from hydrogen peroxide

Author:
Ruan, Jian-wei; Yao, Chen; Bai, Jin-yu; Zhou, Xiao-zhong
Source:
Biochemical and biophysical research communications 2018 v.503 no.2 pp. 607-614
ISSN:
0006-291X
Subject:
plasmids,   etc   ; Adenoviridae; apoptosis; humans; hydrogen peroxide; osteoblasts;     Show all 6 Subjects
Abstract:
... The present study determines the role of the Gab1 in hydrogen peroxide (H2O2)-induced death of human osteoblasts. We show that Gab1 is required for H2O2-induced Akt activation to promote osteoblast survival. In OB-6 human osteoblasts, Gab1 silencing (by targeted-shRNA) or complete knockout (by CRISPR-Cas9 KO plasmid) largely attenuated Akt activation by H2O2. Gab1-depleted OB-6 cells were more vul ...
DOI:
10.1016/j.bbrc.2018.06.048

16. Towards the construction of high-quality mutagenesis libraries

Author:
Li, Heng; Li, Jing; Jin, Ruinan; Chen, Wei; Liang, Chaoning; Wu, Jieyuan; Jin, Jian-Ming; Tang, Shuang-Yan
Source:
Biotechnology letters 2018 v.40 no.7 pp. 1101-1107
ISSN:
0141-5492
Subject:
plasmids,   etc   ; directed evolution; fluorescent proteins; models; mutagenesis;     Show all 5 Subjects
Abstract:
... OBJECTIVES: To improve the quality of mutagenesis libraries in directed evolution strategy. RESULTS: In the process of library transformation, transformants which have been shown to take up more than one plasmid might constitute more than 20% of the constructed library, thereby extensively impairing the quality of the library. We propose a practical transformation method to prevent the occurrence ...
DOI:
10.1007/s10529-018-2559-7

17. Probing the Local Dielectric Constant of Plasmid DNA in Solution and Adsorbed on Chemically Graded Aminosilane Surfaces B

Author:
Li, Zi; Kumarasinghe, Ruwandi; Collinson, Maryanne M.; Higgins, Daniel A.
Source:
The Journal of physical chemistry 2018 v.122 no.8 pp. 2307-2313
ISSN:
1520-5207
Subject:
plasmids,   etc   ; adsorption; dielectric properties; phosphates; silica;     Show all 5 Subjects
Abstract:
... Nile Red dye was used to determine the dielectric constant, ε, of nonpolar microenvironments in double stranded DNA (ds-DNA) both in aqueous buffer solution and when adsorbed on amine-modified surfaces. The value of ε within the DNA decreased with increasing buffer concentration. Values of ε ∼ 6.75 and ∼3.00 were obtained in 0.1 mM phosphate buffered saline (PBS) and in 10 mM PBS, respectively. Si ...
DOI:
10.1021/acs.jpcb.8b00077

18. Compatibility and entry exclusion of IncA and IncC plasmids revisited: IncA and IncC plasmids are compatible

Author:
Ambrose, Stephanie J.; Harmer, Christopher J.; Hall, Ruth M.
Source:
Plasmid 2018 v.96-97 pp. 7-12
ISSN:
0147-619X
Subject:
plasmids,   etc   ; antibiotic resistance genes; nucleotide sequences; terminology;     Show all 4 Subjects
Abstract:
... In an early study, IncA and IncC plasmids that were reported to be compatible were grouped as the “A-C complex” based on similarities and on strong entry exclusion. However, recently, the term IncA/C has been used frequently to describe plasmids belonging to both of these two groups. Granted that the supporting data was not included in the original reports and that the consensus iteron sequences h ...
DOI:
10.1016/j.plasmid.2018.02.002

19. Using overlap‐extension PCR technique to fusing genes for constructing recombinant plasmids

Author:
Lu, Yuanyuan; Xiao, Shuqin; Yuan, Mingyue; Gao, Yibo; Sun, Jiaying; Xue, Chunsheng
Source:
Journal of basic microbiology 2018 v.58 no.3 pp. 273-276
ISSN:
0233-111X
Subject:
plasmids,   etc   ; annealing; genes; polymerase chain reaction; temperature;     Show all 5 Subjects
Abstract:
... Overlap‐extension PCR is a method for splice of gene segments to produce focused fragments for constructing recombinant plasmid, but its complexity limits its application. To simplify the protocol and to improve the effectiveness, we employed gradient temperatures to replace the single annealing temperature in the thermo‐cycling program, and optimize the templates ratio. The concentration of each ...
DOI:
10.1002/jobm.201700455

20. Enhanced production of biosynthesized lycopene via heterogenous MVA pathway based on chromosomal multiple position integration strategy plus plasmid systems in Escherichia coli

Author:
Wei, Yanlong; Mohsin, Ali; Hong, Qi; Guo, Meijin; Fang, Hongqing
Source:
Bioresource technology 2018 v.250 pp. 382-389
ISSN:
0960-8524
Subject:
plasmids,   etc   ; Escherichia coli; gene expression; lycopene; mutants;     Show all 5 Subjects
Abstract:
... The multiple plasmid system, mostly relied, for heterogeneous gene expression, results in genetic instability and low mean productivity. To address this, an integration method was employed for investigating expression of heterogenous pathway in E. coli cells; where mevalonate upper pathway was found efficiently expressed. Subsequently, to improve lycopene production, chromosomal multiple position ...
DOI:
10.1016/j.biortech.2017.11.035