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1. Focused characteristics and effects of light reflected from spherical lipid membrane of giant unilamellar vesicles

Author:
Qiao, Hai; Wei, Zixin; Wang, Yiting; Hu, Na; Sun, Sineng; Bai, Jin; Fang, Liaoqiong; Wang, Zhibiao
Source:
Colloids and surfaces 2020 v.189 pp. 110828
ISSN:
0927-7765
Subject:
plasmids,   etc   ; encapsulation; fluorescence; irradiation; lipids; ultraviolet radiation;     Show all 6 Subjects
Abstract:
... Lipid vesicle is spherical membranous structure with a concave surface on the inside. When a beam of light illuminates a lipid vesicle, the light reflected from the vesicular concave membrane can be focused to have higher intensity and generate enhanced effects. By observing and simulating light reflected from giant unilamellar vesicles (GUVs), the intensity distribution of the light reflected fro ...
DOI:
10.1016/j.colsurfb.2020.110828

2. Design of customizable long linear DNA substrates with controlled end modifications for single-molecule studies

Author:
Mueller, Stefan H.; Spenkelink, Lisanne M.; van Oijen, Antoine M.; Lewis, Jacob S.
Source:
Analytical biochemistry 2020 v.592 pp. 113541
ISSN:
0003-2697
Subject:
plasmids,   etc   ; DNA replication; bacteriophages; chemical reactions;     Show all 4 Subjects
Abstract:
... Many strategies have been developed to manipulate DNA molecules and investigate protein–DNA interactions with single-molecule resolution. Often, these require long DNA molecules with a length of several 10s of kb that are chemically modified at specific regions. This need has traditionally been met by commercially available DNA from bacteriophage λ. However, λ DNA does not allow for the generation ...
DOI:
10.1016/j.ab.2019.113541

3. Plasmid‐chromosome cross‐talks

Author:
Vial, Ludovic; Hommais, Florence
Source:
Environmental microbiology 2020 v.22 no.2 pp. 540-556
ISSN:
1462-2912
Subject:
plasmids,   etc   ; chromosomes; gene expression; mutation; transcription factors;     Show all 5 Subjects
Abstract:
... Plasmids can be acquired by recipient bacteria at a significant cost while conferring them advantageous traits. To counterbalance the costs of plasmid carriage, both plasmids and host bacteria have developed a tight regulatory network that may involve a cross‐talk between the chromosome and the plasmids. Although plasmid regulation by chromosomal regulators is generally well known, chromosome regu ...
DOI:
10.1111/1462-2920.14880

4. Efficient production of HPV16 E2 protein from HPV16 late mRNAs spliced from SD880 to SA2709

Author:
Zheng, Yunji; Cui, Xiaoxu; Nilsson, Kersti; Yu, Haoran; Gong, Lijing; Wu, Chengjun; Schwartz, Stefan
Source:
Virus research 2020 v.285 pp. 198004
ISSN:
0168-1702
Subject:
plasmids,   etc   ; alternative splicing; animal viruses; complementary DNA; messenger RNA;     Show all 5 Subjects
Abstract:
... Human papillomaviruses (HPVs) produce a large number of alternatively spliced mRNAs, including a number of differently spliced mRNAs with the potential to produce E2 protein. To identify the alternatively spliced HPV16 mRNA with the highest ability to produce E2 protein, we have generated E2 cDNA expression plasmids representing the most common, alternatively spliced E2 mRNAs, and assessed their t ...
DOI:
10.1016/j.virusres.2020.198004

5. Multidrug-Resistant Escherichia coli Strain Isolated from Swine in China Harbors mcr-3.1 on a Plasmid of the IncX1 Type That Cotransfers with mcr-1.1

Author:
Sun, Dewei; Jin, Shanshan; Wang, Jing; Wang, Zhenyu; Fan, Jiajian; Xu, Zhengzhong; Xu, Yaohui; Chen, Xiang; Jiao, Xin'an
Source:
Foodborne pathogens & disease 2020
ISSN:
1556-7125
Subject:
plasmids,   etc   ; Escherichia coli; multiple drug resistance; swine; transposases; transposons; China;     Show all 7 Subjects
Abstract:
... An Escherichia coli strain isolated from the feces of swine at a pork slaughterhouse in Henan province China was found to possess two colistin-resistance genes, mcr-1 and mcr-3, plus 16 additional resistance genes. Genes mcr-1.1 and mcr-3.1 were identified on IncHI2 and IncX1 type plasmids, respectively. Transconjugants (containing mcr-3, mcr-1&mcr-3) were obtained that were 64- and 512-fold highe ...
DOI:
10.1089/fpd.2019.2769

6. A frontal analysis combined with a simultaneous chromatographic analysis of macromolecules using a single chromatographic system

Author:
Ambrožič, Rok; Modic, Petra; Hribar, Gorazd; Podgornik, Aleš
Source:
Journal of chromatography 2020 v.1610 pp. 460571
ISSN:
0021-9673
Subject:
plasmids,   etc   ; absorbance; algorithms; cations; chromatography; detection limit; monitoring; monoclonal antibodies;     Show all 8 Subjects
Abstract:
... A chromatographic system was adapted to allow monitoring of eluent of preparative column via absorbance and with the chromatographic analysis of the target macromolecule on the same chromatographic system. The proposed approach was tested on important macromolecules, such as monoclonal antibodies, monoclonal antibody aggregates and plasmid DNA (pDNA). A frontal analysis was made on the preparative ...
DOI:
10.1016/j.chroma.2019.460571

7. Characterisation of a cointegrate plasmid harbouring blaNDM-1 in a clinical Salmonella Lomita strain

Author:
Li, Ruichao; Xie, Miaomiao; Liu, Lizhang; Huang, Yonglu; Wu, Xiaoyan; Wang, Zhiqiang; Chan, Edward Wai Chi; Chen, Sheng
Source:
International journal of antimicrobial agents 2020 v.55 no.1 pp. 105817
ISSN:
0924-8579
Subject:
plasmids,   etc   ; Escherichia coli; Salmonella; anti-infective agents; evolution;     Show all 5 Subjects
Abstract:
... This study aimed to characterise the molecular events underlying formation and evolution of a cointegrate plasmid harbouring blaNDM₋₁ and blaCMY₋₂ in a clinical Salmonella Lomita (S. Lomita) strain. The Salmonella strain SL131 was found to harbour two multidrug resistant (MDR) plasmids. One plasmid, pSL131_IncHI2, is a typical IncHI2 plasmid containing blaOXA₋₁, catB3, arr-3, sul1, qnrB4 and blaDH ...
DOI:
10.1016/j.ijantimicag.2019.09.021

8. Conjugative transfer of Megaplasmids pND6–1 and pND6–2 enhancing naphthalene degradation in aqueous environment: characterization and bioaugmentation prospects

Author:
Wang, Shan; Li, Shanshan; Du, Dan; Wang, Dan; Yan, Wei
Source:
Applied microbiology and biotechnology 2020 v.104 no.2 pp. 861-871
ISSN:
0175-7598
Subject:
plasmids,   etc   ; Pseudomonas putida; bioaugmentation; genes; gentamicin; inoculum; naphthalene;     Show all 7 Subjects
Abstract:
... INTRODUCTION: Naphthalene catabolic strain Pseudomonas putida ND6 harbors two megaplasmids pND6–1 and pND6–2; naphthalene-degrading associated genes are located on pND6–1, while plasmid pND6–2 possesses potential coding genes for conjugative transfer. METHODS: In this study, the recombinant ND6 in which pND6–1 and pND6–2 were labeled with gentamicin and kanamycin-encoding genes, respectively, was ...
DOI:
10.1007/s00253-019-10273-8

9. Immediate, multiplexed and sequential genome engineering facilitated by CRISPR/Cas9 in Saccharomyces cerevisiae

Author:
Li, Zhen-Hai; Meng, Hao; Ma, Bin; Tao, Xinyi; Liu, Min; Wang, Feng-Qing; Wei, Dong-Zhi
Source:
Journal of industrial microbiology & biotechnology 2020 v.47 no.1 pp. 83-96
ISSN:
1367-5435
Subject:
plasmid curing; plasmids,   etc   ; CRISPR-Cas systems; RNA; Saccharomyces cerevisiae; engineering; gene editing; gene overexpression; gene targeting; genes;     Show all 10 Subjects
Abstract:
... A method called Cas-3P allowing for immediate, multiplexed and sequential genome engineering was developed using one plasmid expressing Cas9 and three marked plasmid backbones (P1, P2 and P3) for guide RNA (gRNA) expression. The three marked gRNA plasmid backbones were recurred in a P1–P2–P3 order for sequential gene targeting, without construction of any additional plasmid and elimination of gRNA ...
DOI:
10.1007/s10295-019-02251-w

10. Assessment of Synthesis Machinery of Two Antimicrobial Peptides from Paenibacillus alvei NP75

Author:
Jagadeesan, Yogeswaran; Athinarayanan, Selvamanikandan; Ayub, Sabeena Begum Mohamed; Balaiah, Anandaraj
Source:
Probiotics and antimicrobial proteins 2020 v.12 no.1 pp. 39-47
ISSN:
1867-1306
Subject:
plasmid curing; plasmids,   etc   ; Gram-positive bacteria; Paenibacillus alvei; antibiotics; antimicrobial peptides; antimicrobial properties; bacterial growth; genes; mutants; pathogens; proteinase inhibitors; proteinases; sodium dodecyl sulfate;     Show all 14 Subjects
Abstract:
... Paenibacillus alvei NP75, a Gram-positive bacterium, produces two different antimicrobial peptides, paenibacillin N and P, which has potent antimicrobial activity against many clinical pathogens. The synthesis pattern of these antimicrobial peptides by P. alvei NP75 was studied extensively. The results were outstanding in a way that the paenibacillin N was synthesized irrespective of the growth of ...
DOI:
10.1007/s12602-019-09541-w

11. Protocol: low cost fast and efficient generation of molecular tools for small RNA analysis

Author:
López-Márquez, Diego; Del-Espino, Ángel; Bejarano, Eduardo R.; Beuzón, Carmen R.; Ruiz-Albert, Javier
Source:
Plant methods 2020 v.16 no.1 pp. 41
ISSN:
1746-4811
Subject:
plasmids,   etc   ; endodeoxyribonucleases; gene expression; genes; genetic vectors; microRNA; polymerase chain reaction; small interfering RNA; stress response;     Show all 9 Subjects
Abstract:
... BACKGROUND: Small RNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes such as development, genome stability, or stress response. Functional characterization of sRNAs in plants typically relies on the modification of the steady state levels of these molecules. State-of-the-art strategies to reduce plant sRNA levels include molecular tools suc ...
DOI:
10.1186/s13007-020-00581-w
PubMed:
32206081
PubMed Central:
PMC7082952

12. Particle-mediated delivery of frataxin plasmid to a human sensory neuronal model of Friedreich's ataxia

Author:
Czuba-Wojnilowicz, Ewa; Viventi, Serena; Howden, Sara E.; Maksour, Simon; Hulme, Amy E.; Cortez-Jugo, Christina; Dottori, Mirella; Caruso, Frank
Source:
Biomaterials science 2020 v.8 no.9 pp. 2398-2403
ISSN:
2047-4849
Subject:
plasmids,   etc   ; ataxia (disorder); gene expression; gene therapy; humans; models; neurons; patients; protein content;     Show all 9 Subjects
Abstract:
... Increasing frataxin protein levels through gene therapy is envisaged to improve therapeutic outcomes for patients with Friedreich's ataxia (FRDA). A non-viral strategy that uses submicrometer-sized multilayered particles to deliver frataxin-encoding plasmid DNA affords up to 27 000-fold increase in frataxin gene expression within 2 days in vitro in a stem cell-derived neuronal model of FRDA. ...
DOI:
10.1039/c9bm01757g

13. Virus-esque nucleus-targeting nanoparticles deliver trojan plasmid for release of anti-tumor shuttle protein

Author:
Yang, Suleixin; Ou, Chunqing; Wang, Li; Liu, Xiaowei; Yang, Jin; Wang, Xinxin; Wang, Meilin; Shen, Meiling; Wu, Qinjie; Gong, Changyang
Source:
Journal of controlled release 2020 v.320 pp. 253-264
ISSN:
0168-3659
Subject:
plasmids,   etc   ; coatings; cytotoxicity; encapsulation; gene therapy; genes; nanoparticles; neoplasms; peptides; polyethyleneimine; proteins; transfection;     Show all 12 Subjects
Abstract:
... Gene therapy has gathered vast interest and been proved promising and prospective. While gene therapy evolves fast, demands of high transfecting efficiency and less toxic gene vectors are not sufficiently fulfilled. The progression of materials is doing the favor from which therapeutic application benefited is helping reshape treatments of cancer. In this work, we synthesized fluorinated branched ...
DOI:
10.1016/j.jconrel.2020.01.037

14. Complete genome sequence of Desulfobacter hydrogenophilus AcRS1

Author:
Marietou, Angeliki; Lund, Marie B.; Marshall, Ian P.G.; Schreiber, Lars; Jørgensen, Bo Barker
Source:
Marine genomics 2020 v.50 pp. 100691
ISSN:
1874-7787
Subject:
plasmids,   etc   ; Desulfobacter hydrogenophilus; chromosomes; cold; genome; marine sediments; nucleotide sequences; sulfate-reducing bacteria;     Show all 8 Subjects
Abstract:
... Desulfobacter hydrogenophilus strain AcRS1 is a mesophilic sulfate-reducing bacterium isolated from marine sediment. Here we present the complete genome sequence of D. hydrogenophilus AcRS1 which consists of a circular chromosome of 5,105,135 bp length and two plasmids of 45,346 bp and 6889 bp length. The genome data provide insights into the metabolic potential that allows these sulfate-reducing ...
DOI:
10.1016/j.margen.2019.05.006

15. New approach to identify colistin‐resistant Pseudomonas aeruginosa by high‐resolution melting curve analysis assay

Author:
Tahmasebi, H.; Dehbashi, S.; Arabestani, M.R.
Source:
Letters in applied microbiology 2020 v.70 no.4 pp. 290-299
ISSN:
0266-8254
Subject:
plasmids,   etc   ; Pseudomonas aeruginosa; antibiotics; bacteria; computer software; health services; melting; melting curve analysis; resistance genes; temperature;     Show all 10 Subjects
Abstract:
... Colistin‐resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high‐resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known genomic DNA (gDNA) and plasmid DNA (pDNA) concentrations, extracted from P. aeruginos ...
DOI:
10.1111/lam.13270

16. Comprehensive analysis of the molecular characterization of GM rice G6H1 using a paired-end sequencing approach

Author:
Zhang, Yuchen; Zhang, Hanwen; Qu, Zhan; Zhang, Xiujie; Cui, Jinjie; Wang, Canhua; Yang, Litao
Source:
Food chemistry 2020 v.309 pp. 125760
ISSN:
0308-8146
Subject:
plasmids,   etc   ; Oryza sativa; bioinformatics; chromosomes; crops; genome; polymerase chain reaction; rice; risk assessment; transgenic plants;     Show all 10 Subjects
Abstract:
... Molecular characterization of exogenous DNA integrations in host genome is a key aspect in risk assessment of bioengineered crops. However, gaining a clear understanding of the molecular characters of a bioengineered crop using conventional techniques remains a challenging task. Herein, we report the full molecular characterization of one new transgenic rice event G6H1 via a paired-end sequencing ...
DOI:
10.1016/j.foodchem.2019.125760

17. Exploring the evolutionary dynamics of Rhizobium plasmids through bipartite network analysis

Author:
Li, Xiangchen; Wang, Hao; Tong, Wenjun; Feng, Li; Wang, Lina; Rahman, Siddiq Ur.; Wei, Gehong; Tao, Shiheng
Source:
Environmental microbiology 2020 v.22 no.3 pp. 934-951
ISSN:
1462-2912
Subject:
plasmids,   etc   ; Rhizobium; bacteria; chromosomes; coevolution; essential genes; genetic recombination; host plants; phylogeny;     Show all 9 Subjects
Abstract:
... The genus Rhizobium usually has a multipartite genome architecture with a chromosome and several plasmids, making these bacteria a perfect candidate for plasmid biology studies. As there are no universally shared genes among typical plasmids, network analyses can complement traditional phylogenetics in a broad‐scale study of plasmid evolution. Here, we present an exhaustive analysis of 216 plasmid ...
DOI:
10.1111/1462-2920.14762

18. NetF-producing Clostridium perfringens and its associated diseases in dogs and foals

Author:
Mehdizadeh Gohari, Iman; Unterer, Stefan; Whitehead, Ashley E.; Prescott, John F.
Source:
Journal of veterinary diagnostic investigation 2020 v.32 no.2 pp. 230-238
ISSN:
1943-4936
Subject:
plasmids,   etc   ; Clostridium perfringens; diarrhea; dog diseases; dogs; enteritis; foals; genes; loci; toxigenic strains; toxins; virulence;     Show all 12 Subjects
Abstract:
... The role of type A Clostridium perfringens in canine acute hemorrhagic diarrhea syndrome and foal necrotizing enteritis is poorly characterized. However, a highly significant association between the presence of novel toxigenic C. perfringens and these specific enteric diseases has been described. These novel toxigenic strains produce 3 novel putative toxins, which have been designated NetE, NetF, ...
DOI:
10.1177/1040638720904714
PubMed:
32081091
PubMed Central:
PMC7081511

19. The effects of kanamycin concentration on gene transcription levels in Escherichia coli

Author:
Liu, Xiaozhen; Li, Na; Jia, Minjun; Zhang, Shuangli; Niu, Hao; Li, Qiang; Gu, Pengfei
Source:
3 Biotech 2020 v.10 no.3 pp. 93
ISSN:
2190-572X
Subject:
plasmids,   etc   ; Escherichia coli; antibiotic resistance; enzymes; fermentation; fluorescence; gene overexpression; kanamycin; operon; reporter genes; transcription (genetics);     Show all 11 Subjects
Abstract:
... In the area of microbial production of valuable chemicals, plasmids have been widely applied for overexpressing rate-limiting enzymes with high yields. However, the effect of antibiotic concentrations on the transcription of target genes in E. coli is less involved in previous reports. In this study, we constructed E. coli strains expressing the reporter gene and the kanamycin resistant gene in an ...
DOI:
10.1007/s13205-020-2100-2
PubMed:
32099734
PubMed Central:
PMC7002634

20. Antibiotic resistance genes, class 1 integrons, and IncP-1/IncQ-1 plasmids in irrigation return flows

Author:
Dungan, Robert S.; Bjorneberg, David L.
Source:
Environmental pollution 2020 v.257 pp. 113568
ISSN:
0269-7491
Subject:
plasmids,   etc   ; antibiotic resistance; antibiotic resistance genes; droplets; integrases; irrigation; ribosomal RNA; surface water; watersheds; Idaho;     Show all 10 Subjects
Abstract:
... Surface waters could be a dominant route by which antibiotic resistance genes (ARGs) are disseminated. In the present study we explored the prevalence and abundance of ARGs [blaCTX₋M₋₁, erm(B), sul1, tet(B), tet(M), and tet(X)], class 1 integron-integrase gene (intI1), and IncP-1 and IncQ-1 plasmids in eight irrigation return flows (IRFs) and a background site (Main Line Canal, MLC) in the Upper S ...
DOI:
10.1016/j.envpol.2019.113568