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plasmids, etc ; biotechnology; detection limit; food safety; genetically modified organisms; immunoassays; markets; rapid methods; Show all 8 Subjects
Abstract:
... Advanced molecular biotechnology has been developed to provide favorable technical support for genetically modified (GM) crop detection. For sensitive on-site detection, a Cas12a-based immunoassay strip was self-prepared and evaluated in this study. The strip was applied to analyze transgenic screening elements P-CaMV 35S and T-NOS by combination with RPA and Cas12a cleavage reaction. The assay ca ...
plasmids, etc ; CRISPR-Cas systems; Larimichthys crocea; aquaculture; chorion; disease resistance; genes; genetic improvement; genomics; green fluorescent protein; meat quality; mutants; survival rate; China; Show all 14 Subjects
Abstract:
... The large yellow croaker (Larimichthys crocea) is an economically important marine species with the highest annual production among the farmed marine fishes in China. However, the aquaculture industry of this species is suffering from severe problems that include weakened disease resistance, decreased growth rate, and reduced meat quality due to frequent inbreeding. Genome editing, which has a hug ...
... As a new type of environmental pollutant, antibiotic resistance genes (ARGs) pose a huge challenge to global health. Horizontal gene transfer (HGT) represents an important route for the spread of ARGs. The widespread use of sulfamethazine (SM2) as a broad-spectrum bacteriostatic agent leads to high residual levels in the environment, thereby increasing the spread of ARGs. Therefore, we chose to st ...
plasmids, etc ; Achromobacter; Mycobacterium; Stenotrophomonas; ammonia; antibiotic resistance; antibiotic resistance genes; antibiotics; autumn; biofilm; community structure; hardness; metagenomics; microbial communities; plate count; seasonal variation; spring; suburban areas; summer; temperature; total organic carbon; water distribution; water treatment; winter; China; Show all 25 Subjects
Abstract:
... Antibiotic resistance genes (ARGs) are an emerging issue for drinking water safety. However, the seasonal variation of ARGs in drinking water distribution systems (DWDS) is still unclear. This work revealed the tempo-spatial changes of microbial community, ARGs, mobile genetic elements (MGEs) co-occurring with ARGs, ARG hosts in DWDS bulk water by means of metagenome assembly. The microbial commun ...
... Gram-negative bacteria are prevalent pathogens associated with hospital-acquired infections (HAI) that are a major challenge for patient safety, especially in intensive care units [...] ...
... Here, we develop a simple, efficient, bacmid-based, selection marker-free method for gene deletion and editing in baculovirus genomes. Specifically, based on pFastbac1, a donor plasmid with long left and right homology arms but without a reporter was constructed for disrupting ie1, an essential baculovirus gene. Instead of ligating with a plasmid, the homology arms were introduced to the polyhedri ...
plasmids, etc ; Markov chain; metagenomics; molecular biology; Show all 4 Subjects
Abstract:
... Machine learning or deep learning models have been widely used for taxonomic classification of metagenomic sequences and many studies reported high classification accuracy. Such models are usually trained based on sequences in several training classes in hope of accurately classifying unknown sequences into these classes. However, when deploying the classification models on real testing data sets, ...
plasmids, etc ; Acinetobacter baumannii; bioinformatics; host range; Show all 4 Subjects
Abstract:
... The bioinformatic analysis that we made of 492 Acinetobacter baumannii plasmid sequences identified 418 genes encoding Replication Initiator (Rep) proteins that fell into at least fourteen groups according to the protein domains that they contained. The most abundant group of Rep proteins contained a Rep_3 superfamily domain, followed by Rep proteins containing Replicase/PriCT_1 superfamily domain ...
plasmids, etc ; agarose; biosynthesis; gels; microRNA; nanowires; Show all 6 Subjects
Abstract:
... Multiple microRNA (miRNA) logical assays have attracted wide attention recently, which can be applied to mimic and reveal cellular events at the molecular level. However, it remains challenging to develop labeling- and amplification-free approaches to perform logical functions with low levels of miRNA molecules. Herein, we proposed a strategy for miRNA logic operations using plasmid DNA-derived na ...
plasmids, etc ; biotechnology; methyltransferases; prokaryotic cells; restriction endonucleases; Show all 5 Subjects
Abstract:
... Since prokaryotic restriction-modification (RM) systems protect the host by cleaving foreign DNA by restriction endonucleases, it is difficult to introduce engineered plasmid DNAs into newly isolated microorganisms whose RM system is not discovered. The prokaryotes also possess methyltransferases to protect their own DNA from the endonucleases. As those methyltransferases can be utilized to methyl ...
plasmids, etc ; flow cytometry; glucose; lipids; oxygen; transfection; Show all 6 Subjects
Abstract:
... The amino groups in the head group of a cationic lipid play a determinative role regarding the nucleic acid delivery efficiency of the LNP formulated from lipids. Herein, we designed four types of lipid bearing different amine-containing branched head groups to investigate the influence of type and number of amines on the neural cell targeted nuclei acid delivery. Conjugation of an ethylamino grou ...
plasmids, etc ; Agrobacterium; crown galls; oncogenes; phylogeny; virulence; Show all 6 Subjects
Abstract:
... Tumor-inducing (Ti) and root-inducing (Ri) plasmids of Agrobacterium that display a large diversity are involved in crown gall and hairy root plant diseases. Their phylogenetic relationships were inferred from an exhaustive set of Ti and Ri plasmids (including 36 new complete Ti plasmids) by focusing on T-DNA and virulence regions. The opine synthase gene content of T-DNAs revealed 13 opine types ...
plasmids, etc ; drugs; dyes; genes; humans; toxicity; yeasts; Show all 7 Subjects
Abstract:
... AIMS: This study aimed to establish a yeast‐based screening system for potential compounds that can alleviate the toxicity of α‐synuclein (α‐syn), a neuropathological hallmark of Parkinson’s disease, either inhibition of α‐syn aggregation or promotion of ubiquitin‐mediated degradation of α‐syn. METHODS AND RESULTS: A powerful yeast‐based screening assay using the rsp5ᴬ⁴⁰¹ᴱ‐mutant strain, which is ...
plasmids, etc ; Western blotting; humans; polyethyleneimine; puromycin; ultracentrifugation; Show all 6 Subjects
Abstract:
... The current protocol describes the use of lentiviral particles for the delivery of short hairpin RNAs (shRNAs) to both human embryonic stem cells (hESCs) as well as neural progenitor cells (NPCs) derived from hESCs at high efficiency. Lentiviral particles were generated by co-transfecting HEK293T cells using entry vectors (carrying shRNAs) along with packaging plasmids (pAX and pMD2.G) using the l ...
Alice Wegener; Birgitta Duim; Linda van der Graaf-van Bloois; Aldert L. Zomer; Caroline E. Visser; Mirlin Spaninks; Arjen J. Timmerman; Jaap A. Wagenaar; Els M. Broens
plasmids, etc ; Staphylococcus pseudintermedius; antibiotic resistance; genome; humans; Show all 5 Subjects
Abstract:
... Staphylococcus pseudintermedius can be transmitted between dogs and their owners and can cause opportunistic infections in humans. Whole genome sequencing was applied to identify the relatedness between isolates from human infections and isolates from dogs in the same households. Genome SNP diversity and distribution of plasmids and antimicrobial resistance genes identified related and unrelated i ...
Federica Sacco; Giammarco Raponi; Alessandra Oliva; Giulia Bibbolino; Vera Mauro; Federica Maria Di Lella; Lorenzo Volpicelli; Guido Antonelli; Mario Venditti; Alessandra Carattoli; Gabriele Arcari
plasmids, etc ; Klebsiella pneumoniae; aminoglycosides; genes; genomics; methyltransferases; Show all 6 Subjects
Abstract:
... The spread of extremely-drug-resistant Klebsiella pneumoniae has become a major health threat worldwide. This is largely mediated by certain lineages, recognized as high-risk clones dispersed throughout the world. Analysis of an outbreak of nine ST15, NDM-1 metallo-β-lactamase-producing K. pneumoniae was performed. An IncC plasmid carrying the blaNDM₋₁ gene also carried the rare rmtC gene, encodin ...
plasmids, etc ; Yersinia enterocolitica; cold; rapid methods; virulence; yersiniosis; Show all 6 Subjects
Abstract:
... Yersinia enterocolitica and Y. pseudotuberculosis are important causes of enteric illness worldwide. Rapid response to suspected foodborne outbreaks is hampered by the widespread use of cold enrichment methods that require incubation periods of 10–21 days. Although these species grow faster at elevated temperatures, part of the rationale for cold enrichment is that a key pathogenicity marker (pYV ...
plasmids, etc ; Kluyveromyces marxianus; centromeres; cytotoxicity; recombinases; reporter genes; Show all 6 Subjects
Abstract:
... The Cre-loxP system produces structural variations, such as deletion, duplication, inversion and translocation, at specific loci and induces chromosomal rearrangements in the genome. To achieve chromosomal rearrangements in Kluyveromyces marxianus, the positions and sequences of centromeres were identified in this species for the first time. Next, a Cre-loxP system was established in K. marxianus. ...
plasmids, etc ; Klebsiella oxytoca; drug resistance; hospitals; microbiology; China; Show all 6 Subjects
Abstract:
... Carbapenem-resistant Klebsiella michiganensis (CRKM) and Klebsiella oxytoca (CRKO) strains have occasionally been reported to cause severe infections. However, SIM-producing K. michiganensis strains have never been described. In this study, we phenotypically and genetically characterized 6 CRKM and CRKO strains isolated over the past 10 years at a Chinese tertiary hospital. All six strains were po ...
plasmids, etc ; antibiotic resistance; databases; excision; multigene family; transposases; Show all 6 Subjects
Abstract:
... Traditionally, insertion sequences (ISs) play a major role in disseminating antimicrobial resistance genes (ARGs) in bacteria through transposition and translocation, forming regions that contain multiple ARGs flanked by single or multiple copies of IS. In addition, unconventional circularizable structures (UCSs), lacking recombinase genes but being surrounded by directly repeated sequences (DRs) ...