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1. Designing an aptamer based magnetic and upconversion nanoparticles conjugated fluorescence sensor for screening Escherichia coli in food

Author:
Li, Huanhuan; Ahmad, Waqas; Rong, Yawen; Chen, Quansheng; Zuo, Min; Ouyang, Qin; Guo, Zhiming
Source:
Food control 2020 v.107 pp. 106761
ISSN:
0956-7135
Subject:
plate count,   etc   ; Escherichia coli; adulterated foods; crystal structure; detection limit; fluorescence; magnetism; nanoparticles; oligonucleotides; pork; screening;     Show all 11 Subjects
Abstract:
... The study assembles a highly sensitive upconversion fluorescence sensor for screening Escherichia coli (E. coli) in food. The principle of the strategy was based on the specific matching between two aptamers embedded magnetic nanoparticles (MNPs) and cDNA-upconversion nanoparticles (UCNPs). The fluorescence emission intensity of the MNPs-aptamer-cDNA-UCNPs conjugate system gradually decreased upon ...
DOI:
10.1016/j.foodcont.2019.106761

2. Optimization of spiral continuous flow-through pulse light sterilization for Escherichia coli in red grape juice by response surface methodology

Author:
Xu, Feifei; Wang, Bei; Hong, Chen; Telebielaigen, Shabuerbieke; Nsor-Atindana, John; Duan, Yuqing; Zhong, Fang
Source:
Food control 2019 v.105 pp. 8-12
ISSN:
0956-7135
Subject:
plate count,   etc   ; Escherichia coli; grape juice; response surface methodology;     Show all 4 Subjects
Abstract:
... The effect of a spiral continuous flow-through pulsed light (PL) system on the inactivation of Escherichia coli in red grape juice was evaluated. The grape juice was treated with different numbers of pulses, intensities of PL and liquid flow rates. Reduction of E. coli was determined by plate counting method. Response surface methodology was used to analyze the correlation among the factors. The r ...
DOI:
10.1016/j.foodcont.2019.04.023

3. A poly(glycidylmethacrylate-divinylbenzene)-based anion exchanger for ion chromatography

Author:
Yang, Zhanqiang; Gao, Min; Li, Zongying; Zhang, Feifang; Zhang, Shengmao; Yang, Bingcheng
Source:
Journal of chromatography 2019 v.1596 pp. 79-83
ISSN:
0021-9673
Subject:
plate count,   etc   ; anions; chemical bonding; hydrolysis; hydrophilicity; ion exchange chromatography;     Show all 6 Subjects
Abstract:
... An anion exchanger has been described by grafting dimethylaminoethyl methacrylate methylchloride (DMC) onto the poly(glycidyl methacrylate-divinylbenzene) (GMA-DVB) subtract. The grafting was performed by coupling of DMC with the pendant double bonds of DVB. Prior to grafting, a hydrolysis treatment to free epoxide groups associated with GMA-DVB substrate enables an improved hydrophilic surface of ...
DOI:
10.1016/j.chroma.2019.02.062

4. Apparent efficiency of serially coupled columns in isocratic and gradient elution modes

Author:
Fekete, Szabolcs; Codesido, Santiago; Rudaz, Serge; Guillarme, Davy; Horváth, Krisztián
Source:
Journal of chromatography 2018 v.1571 pp. 121-131
ISSN:
0021-9673
Subject:
plate count,   etc   ; chromatography; particle size; prediction;     Show all 4 Subjects
Abstract:
... The goal of this work was to understand the variation of apparent efficiency when serially coupling columns with identical stationary phase chemistries, but with differences in their kinetic performance. For this purpose, a mathematical treatment was developed both for isocratic and gradient modes to assess the change in plate numbers and peak widths when coupling arbitrary several columns. To val ...
DOI:
10.1016/j.chroma.2018.08.002

5. Estimation of peak capacity based on peak simulation A

Author:
Navarro-Huerta, J.A.; Torres-Lapasió, J.R.; García-Alvarez-Coque, M.C.
Source:
Journal of chromatography 2018
ISSN:
0021-9673
Subject:
plate count,   etc   ; chemical species; chromatography; equations; solutes;     Show all 5 Subjects
Abstract:
... Peak capacity (PC) is a key concept in chromatographic analysis, nowadays of great importance for characterising complex separations as a criterion to find the most promising conditions. A theoretical expression for PC estimation can be easily deduced in isocratic elution, provided that the column plate count is assumed constant for all analytes. In gradient elution, the complex dependence of peak ...
DOI:
10.1016/j.chroma.2018.09.009

6. Reduction of the extra-column band dispersion by a slow transport of a sample band from the injector to the column in isocratic reversed-phase liquid chromatography A

Author:
Kuroda, Ikuma; Uzu, Hideyuki; Miyazaki, Shota; Ohira, Masayoshi; Tanaka, Nobuo
Source:
Journal of chromatography 2018
ISSN:
0021-9673
Subject:
plate count,   etc   ; reversed-phase liquid chromatography; solutes;     Show all 3 Subjects
Abstract:
... Extra-column band dispersion during the transport of a sample band from the injector to the column can be reduced by a flow rate program starting with a low flow rate until the sample band has approached to, or just entered into the column, followed by an increased flow rate suitable for the solute separation in the column. Such a sample introduction method increased the plate counts of a 50 mm lo ...
DOI:
10.1016/j.chroma.2018.08.037

7. Are some teat disinfectant formulations more effective against specific bacteria isolated on teat skin than others?

Author:
Fitzpatrick, Sarah Rose; Garvey, Mary; Flynn, Jim; Jordan, Kieran; Gleeson, David
Source:
Acta veterinaria scandinavica 2019 v.61 no.1 pp. 21
ISSN:
1751-0147
Subject:
plate count,   etc   ; Holstein; Staphylococcus; Streptococcus; coliform bacteria; cows; disinfectants; iodine; microbial load; milk; milking; teats;     Show all 12 Subjects
Abstract:
... The use of pre- and post-milking teat disinfectants can reduce teat bacterial load and aid in the collection of high-quality milk. The objective of this study was to compare the reduction in bacteria populations on teat skin after the application of different commercial teat disinfectant products. Ten teat disinfectant products were applied to the teats of 10 Holstein–Friesian cows. One cow receiv ...
DOI:
10.1186/s13028-019-0455-3

8. Quality characteristics of fresh wet noodles treated with nonthermal plasma sterilization

Author:
Chen, Yue; Chen, Guiyun; Wei, Rui; Zhang, Yifu; Li, Shuhong; Chen, Ye
Source:
Food chemistry 2019 v.297 pp. 124900
ISSN:
0308-8146
Subject:
plate count,   etc   ; acidity; color; fungi; nonthermal processing; noodles; nuclear magnetic resonance spectroscopy; texture; water activity;     Show all 9 Subjects
Abstract:
... Nonthermal plasma treatment has been a widely acknowledged effective method for food sterilization. This study presents the potential of nonthermal dielectric barrier discharge (DBD) plasma treatment for sterilization of the surface of the fresh wet noodles (FWNs). The plasma treatment achieved a more than one logarithmic reduction of the total bacterial count and total mold count as measured by c ...
DOI:
10.1016/j.foodchem.2019.05.174

9. Zinc fortification as a tool for improving sprout hygienic and nutritional quality: a factorial design approach

Author:
Jribi, Sarra; Molnàr, Helga; Antal, Otilia Tamara; Adànyi, Nóra; Kheriji, Oussema; Naàr, Zoltan; Debbabi, Hajer
Source:
Journal of the science of food and agriculture 2019 v.99 no.11 pp. 5187-5194
ISSN:
0022-5142
Subject:
plate count,   etc   ; coliform bacteria; flora; hygiene; molds (fungi); nutritive value; soaking; sprouting; temperature; wheat; yeasts; zinc;     Show all 12 Subjects
Abstract:
... BACKGROUND: Sprouting is known to improve cereal and pulse nutritional properties. However, several outbreaks of illness have been reported after raw sprout consumption. This research aimed to improve wheat sprout hygienic properties through the use of zinc diacetate. Sprouting conditions (sprouting temperature, soaking time and zinc diacetate solution concentration) were optimized to decrease tot ...
DOI:
10.1002/jsfa.9765

10. Near-infrared LEDS provide persistent and increasing protection against E. faecalis

Author:
Petrini, M.; Spoto, G.; Scarano, A.; D'Arcangelo, C.; Tripodi, D.; Di Fermo, P.; D'Ercole, S.
Source:
Journal of photochemistry and photobiology 2019 v.197 pp. 111527
ISSN:
1011-1344
Subject:
plate count,   etc   ; Enterococcus faecalis; analysis of variance; fluorescence microscopes; irradiation; light emitting diodes; sodium hypochlorite; viability;     Show all 8 Subjects
Abstract:
... In a previous study, we have shown that non-coherent light-emitting diodes (LEDs) are able to significantly decrease Enterococcus faecalis viability, in vitro. In particular, the concurrent treatment for 5 min with sodium hypochlorite (NaOCl) 1.0% and LED permitted to obtain the total inactivation of the culture.The primary outcome of this study was to verify if the photoinactivation was only temp ...
DOI:
10.1016/j.jphotobiol.2019.111527

11. Online assessment of sand filter performance for bacterial removal in a full-scale drinking water treatment plant

Author:
Fujioka, Takahiro; Ueyama, Tetsuro; Mingliang, Fang; Leddy, Menu
Source:
Chemosphere 2019 v.229 pp. 509-514
ISSN:
0045-6535
Subject:
plate count,   etc   ; bacteria; counters; drinking water; drinking water treatment; flocculation; monitoring; risk; sand filters; water quality;     Show all 10 Subjects
Abstract:
... Microbiological risks associated with drinking water can be minimized by providing enhanced integrity monitoring of bacterial removal by water treatment processes. This study aimed to evaluate the efficacy of real-time bacteriological counters for continuously assessing the performance of a full-scale sand filter to remove bacteria. Over the course of an 8-day evaluation, online counting of bacter ...
DOI:
10.1016/j.chemosphere.2019.04.197

12. Development of a flow cytometric assay to assess the bacterial count in boar semen

Author:
Oehler, Christin; Janett, Fredi; Schmitt, Sarah; Malama, Eleni; Bollwein, Heinrich
Source:
Theriogenology 2019 v.133 pp. 125-134
ISSN:
0093-691X
Subject:
plate count,   etc   ; bacteria; boars; flow cytometry; most probable number technique; propidium; semen; staining;     Show all 8 Subjects
Abstract:
... The aim of the study was to develop a new flow cytometric assay for the determination of the bacterial count in commercially processed boar semen.In total 224 fresh boar semen samples collected at an AI-station were analyzed. The number of total viable counts (TVC) was determined by using flow cytometry after staining with SYBR Green I and Propidium Iodide (PI). In the first part of the study 111 ...
DOI:
10.1016/j.theriogenology.2019.04.036

13. Decontamination of knives used in a slaughterhouse by a commercial non-thermal UV-C treatment

Author:
Raschle, Susanne; Zweifel, Claudio; Zurfluh, Katrin; Stephan, Roger
Source:
Italian journal of food safety 2019 v.8 no.2
ISSN:
2239-7132
Subject:
plate count,   etc   ; Escherichia coli; Staphylococcus aureus; antibacterial properties; blood; cleaning; decontamination; knives; slaughterhouses; ultraviolet radiation;     Show all 10 Subjects
Abstract:
... To assess the antimicrobial effect of a commercial UV-C system, knives inoculated with Escherichia coli and Staphylococcus aureus as well as naturally contaminated and collected from the wet and clean area of a slaughterhouse knives were examined. For inoculated knives, UVC treatment for 30 s reduced mean E. coli counts by 5.1 log CFU cm⁻² and mean S. aureus counts by 4.5 log CFU cm⁻². The presenc ...
DOI:
10.4081/ijfs.2019.8107

14. Efficacy of ceftolozane/tazobactam, alone and in combination with colistin, against multidrug-resistant Pseudomonas aeruginosa in an in vitro biofilm pharmacodynamic model

Author:
Gómez-Junyent, Joan; Benavent, Eva; Sierra, Yanik; El Haj, Cristina; Soldevila, Laura; Torrejón, Benjamín; Rigo-Bonnin, Raul; Tubau, Fe; Ariza, Javier; Murillo, Oscar
Source:
International journal of antimicrobial agents 2019 v.53 no.5 pp. 612-619
ISSN:
0924-8579
Subject:
plate count,   etc   ; Pseudomonas aeruginosa; bacteria; biofilm; ceftazidime; colistin; meropenem; models; multiple drug resistance; pharmacodynamics; regrowth; therapeutics;     Show all 12 Subjects
Abstract:
... Ceftolozane/tazobactam is a potential tool for infections caused by multidrug-resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa), but its efficacy against some difficult-to-treat infections has not been well defined.Using an in vitro pharmacodynamic biofilm model, this study evaluated the comparative efficacy of ceftolozane/tazobactam against MDR/extensively drug-resistant (XDR) P. aeruginosa ...
DOI:
10.1016/j.ijantimicag.2019.01.010

15. Evidence of Signatures for Recognition of Hydrocarbon Microseepage in El Hajeb Oilfield, Eastern Tunisia

Author:
Mahmoud Bougi, Mohamed Seddik; Baklouti, Syrine; Rasheed, M. A.; Rao, P. L. Srinivasa; Hasan, Syed Zaheer; Gharbi, Jawhar; Ksibi, Mohamed
Source:
Geomicrobiology journal 2019 v.36 no.4 pp. 348-358
ISSN:
1521-0529
Subject:
plate count,   etc   ; bacteria; basins; oil fields; petroleum; seepage; soil air; soil sampling; Tunisia;     Show all 9 Subjects
Abstract:
... Microbial prospecting technique was applied in El Hajeb oilfield, Tunisia for potential hydrocarbon resource exploration by examining the anomalous abundance of hydrocarbon oxidizing bacteria. The method depends on the seepage of light gaseous hydrocarbons such as C1 – C5 from the petroleum pools to the shallow surface giving the reasonable conditions to the development of highly specialized bacte ...
DOI:
10.1080/01490451.2018.1555294

16. A low-cost, open-source digital stripchart recorder for chromatographic detectors using a Raspberry Pi

Author:
Foster, Samuel W.; Alirangues, Michael J.; Naese, Joseph A.; Constans, Eric; Grinias, James P.
Source:
Journal of chromatography 2019
ISSN:
0021-9673
Subject:
plate count,   etc   ; affordability; chromatography; computer software; computers; data collection; detectors; electronic circuits; raspberries; user interface;     Show all 10 Subjects
Abstract:
... One of the most critical aspects of chromatographic analysis is effective data acquisition and processing. Typical approaches include software platforms designed for specific instruments or commercial data acquisition hardware boards, both of which require expensive licenses to use and operate. To increase the access and affordability of chromatographic data acquisition, especially for systems in ...
DOI:
10.1016/j.chroma.2019.03.070

17. Isolation and characterization of a bacteriophage and its potential to disrupt multi-drug resistant Pseudomonas aeruginosa biofilms

Author:
Yuan, Yuyu; Qu, Kunli; Tan, Demeng; Li, Xiaoyu; Wang, Lili; Cong, Cong; Xiu, Zhilong; Xu, Yongping
Source:
Microbial pathogenesis 2019 v.128 pp. 329-336
ISSN:
0882-4010
Subject:
plate count,   etc   ; DNA; Pseudomonas aeruginosa; bacteriophages; biofilm; enzymes; genome; multiple drug resistance; scanning electron microscopy; therapeutics;     Show all 10 Subjects
Abstract:
... A lytic Pseudomonas aeruginosa bacteriophage, vB_PaeM_LS1, was isolated and characterized herein. To examine the eligibility of bacteriophage vB_PaeM_LS1 as a therapeutic bacteriophage, we analysed its genome and compared it to similar bacteriophages. Genome of bacteriophage vB_PaeM_LS1 consisted of a linear, double-stranded DNA molecule 66,095 bp in length and with 55.7% G + C content. Neighbor-j ...
DOI:
10.1016/j.micpath.2019.01.032

18. Fluorescence microscopy to monitor wine malolactic fermentation

Author:
Fernández-Pérez, Rocío; Tenorio Rodríguez, Carmen; Ruiz-Larrea, Fernanda
Source:
Food chemistry 2019 v.274 pp. 228-233
ISSN:
0308-8146
Subject:
plate count,   etc   ; cell viability; deacidification; fluorescence microscopy; lactic acid bacteria; malolactic fermentation; red wines; staining;     Show all 8 Subjects
Abstract:
... Malolactic fermentation (MLF) is a natural and biological deacidification of wines and a required step for making premium red wines. MLF is carried out by lactic acid bacteria (LAB) that are present in the fermenting wines. Currently, real-time control of MLF is an issue of great interest as the classical plate count technique for assessing bacterial populations requires long incubation times that ...
DOI:
10.1016/j.foodchem.2018.08.088

19. Predicting heat process efficiency in thermal processes when bacterial inactivation is not log-linear

Author:
Desriac, N.; Vergos, M.; Achberger, V.; Coroller, L.; Couvert, O.
Source:
International journal of food microbiology 2019 v.290 pp. 36-41
ISSN:
0168-1605
Subject:
plate count,   etc   ; Bacillus pumilus; food industry; heat inactivation; heat treatment; models; prediction; temperature profiles;     Show all 8 Subjects
Abstract:
... The food industry widely uses the F-value which considers microbial log-linear inactivation, while microbial heat inactivation may result in a non-log-linear inactivation pattern due to genetic or phenotypical heterogeneity. This may yield discrepancies in predicting microbial heat inactivation under dynamic conditions of heat treatment. In this paper, we suggest the calculation of the equivalent ...
DOI:
10.1016/j.ijfoodmicro.2018.09.028

20. Effect of expiry date communication on acceptability and waste of fresh-cut lettuce during storage at different temperatures

Author:
Alongi, Marilisa; Sillani, Sandro; Lagazio, Corrado; Manzocco, Lara
Source:
Food research international 2019 v.116 pp. 1121-1125
ISSN:
0963-9969
Subject:
plate count,   etc   ; color; fresh-cut produce; lettuce; pouches; risk; storage temperature; storage time; wastes;     Show all 9 Subjects
Abstract:
... The effect of expiry date communication on acceptability and wasting risk of fresh-cut lettuce was investigated. Fresh-cut lettuce was packed in plastic pouches reporting or not the expiry date on the label and stored at recommended (8 °C) or abuse temperature (12 °C) for increasing time up to 21 days. Lettuce was assessed during storage for colour, total viable count, consumer rejection and wasti ...
DOI:
10.1016/j.foodres.2018.09.056