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1. Dark-field visual counting of white spot syndrome virus using gold nanoparticle probe

Author:
Yanan Bai; Le He; Mengling Sun; Xin Zhou; Zhiqiang Xu
Source:
Aquaculture 2023 v.562 pp. 738797
ISSN:
0044-8486
Subject:
polyclonal antibodies,   etc   ; White spot syndrome virus; aquaculture; detection limit; nanogold; pathogens; quantitative polymerase chain reaction; shrimp; shrimp fisheries;     Show all 9 Subjects
Abstract:
... White spot syndrome virus (WSSV) is an important pathogen in shrimp farming and causes serious economic losses in the shrimp industry. Early detection of WSSV is particularly important in the prevention and treatment of the disease. However, the current routine technology lacks the convenience, ultra-sensitivity and speed that have limited the development of on-site detection application of WSSV. ...
DOI:
10.1016/j.aquaculture.2022.738797

2. Rapid and automatic Salmonella typhimurium detection integrating continuous-flow magnetic separation and dynamic impedance measurement

Author:
Li Xue; Ruya Guo; Nana Jin; Siyuan Wang; Hong Duan; Wuzhen Qi; Lei Wang; Yuanjin Zheng; Yanbin Li; Jianhan Lin
Source:
Food control 2023 v.143 pp. 109316
ISSN:
0956-7135
Subject:
polyclonal antibodies,   etc   ; Salmonella Typhimurium; chickens; detection limit; electrodes; food safety; gluconic acid; glucose; glucose oxidase; hydrogen peroxide; magnetic fields; magnetic separation; mobile telephones; monoclonal antibodies; organ-on-a-chip; polystyrenes;     Show all 16 Subjects
Abstract:
... Screening of pathogenic bacteria is an essential measure for ensuring food safety. A fast, sensitive and automatic platform for Salmonella determination was presented employing continuous-flow magnetic isolation, enzymatic impedance amplification and smartphone data analysis. First, magnetic nanoparticles coupling with polyclonal antibody, polystyrene spheres coupling with monoclonal antibody and ...
DOI:
10.1016/j.foodcont.2022.109316

3. Development and validation of a specific sandwich ELISA for determination of soybean allergens and its application in processed foods

Author:
Liyan Zhu; Siyue Li; Lirui Sun; Jinlong Zhao; Jianlian Huang; Yinmei Jiang; Shuo Wan; Tushar Ramesh Pavase; Zhenxing Li
Source:
Process biochemistry 2022 v.117 pp. 134-141
ISSN:
1359-5113
Subject:
polyclonal antibodies,   etc   ; allergens; cross reaction; enzyme-linked immunosorbent assay; goats; rabbits; soybeans;     Show all 7 Subjects
Abstract:
... Soybean allergens are commonly quantified using enzyme-linked immunosorbent assays (ELISAs) with antibodies produced by the single allergen. To enhance the specificity and avoid the false-negative results, the sandwich ELISA method was developed using a rabbit anti-bulk soybean proteins polyclonal antibody and a goat anti- bulk soybean proteins polyclonal antibody as the detection and capture anti ...
DOI:
10.1016/j.procbio.2022.03.022

4. Quantum-Dot-Bead-Based Fluorescence-Linked Immunosorbent Assay for Sensitive Detection of Cry2A Toxin in Cereals Using Nanobodies

Author:
Yulou Qiu; Ajuan You; Xianshu Fu; Mingzhou Zhang; Haifeng Cui; Biao Zhang; Weiwei Qin; Zihong Ye; Xiaoping Yu
Source:
Foods 2022 v.11 no.18 pp. -
ISSN:
2304-8158
Subject:
polyclonal antibodies,   etc   ; colorimetry; detection limit; enzyme-linked immunosorbent assay; fluorescent dyes; toxins;     Show all 6 Subjects
Abstract:
... In this study, a quantum-dot-bead (QB)-based fluorescence-linked immunosorbent assay (FLISA) using nanobodies was established for sensitive determination of the Cry2A toxin in cereal. QBs were used as the fluorescent probe and conjugated with a Cry2A polyclonal antibody. An anti-Cry2A nanobody P2 was expressed and used as the capture antibody. The results revealed that the low detection limit of t ...
DOI:
10.3390/foods11182780

5. Robust humoral immune response against rabies virus in rabbits and guinea pigs immunized with plasmid DNA vectors encoding rabies virus glycoproteins – An approach to the production of polyclonal antibody reagents

Author:
Dmitriy V. Volokhov; Vyacheslav Furtak; Cynthia Allen; Gayle Pulle; Michelle D. Zajac; Yotam Levin; Efrat Kochba; Susan M. Moore
Source:
Molecular and cellular probes 2022 v.64 pp. 101833
ISSN:
0890-8508
Subject:
polyclonal antibodies,   etc   ; Rabies lyssavirus; fluorescence; glycoproteins; humoral immunity; immunization; plasmids; rabbits; rabies; viruses; Guinea;     Show all 11 Subjects
Abstract:
... DNA-based immunization has been previously shown to be an efficient approach to induce robust immunity against infectious diseases in animals and humans. The advantages of DNA vaccines are simplicity of their construction and production, low cost, high stability, and ability to elicit a full spectrum of immune responses to target antigens. The goals of this study were (i) to assess the antibody im ...
DOI:
10.1016/j.mcp.2022.101833

6. Characterization of rabbit polyclonal antibody against camel recombinant nanobodies

Author:
Houssam-Eddin Khalaf; Hassan Al-Bouqaee; Manal Hwijeh; Abdul Qader Abbady
Source:
Open life sciences 2022 v.17 no.1 pp. 659-675
ISSN:
2391-5412
Subject:
polyclonal antibodies,   etc   ; Camelidae; affordability; camels; enzyme-linked immunosorbent assay; epitopes; immunization; plasmids; rabbits; solubility;     Show all 10 Subjects
Abstract:
... Nanobodies (Nbs) are recombinant single-domain fragments derived from camelids’ heavy-chain antibodies (HCAbs). Nanobodies are increasingly used in numerous biotechnological and medical applications because of their high stability, solubility, and yield. However, one major obstacle prohibiting Nb expansion is the affordability of specific detector antibodies for their final revelation. In this wor ...
DOI:
10.1515/biol-2022-0065

7. A simple electrochemical immunosensor for sensitive detection of transgenic soybean protein CP4-EPSPS in seeds

Author:
Marcos E. Farías; N. Mariano Correa; Lucas Sosa; Ana M. Niebylski; Patricia G. Molina
Source:
Talanta 2022 v.237 pp. 122910
ISSN:
0039-9140
Subject:
polyclonal antibodies,   etc   ; detection limit; electrochemistry; electrodes; glyphosate; glyphosate resistance; gold; immunosensors; soy protein; soybeans; Argentina;     Show all 11 Subjects
Abstract:
... Soybean is the most produced crop in Argentina, and 99 % corresponds to genetically modified soybean. One of the main produced varieties is Roundup Ready® soybean (RR), which was modified to express the enzyme CP4 5-enolpyruvylshikimate 3-phosphate synthase (CP4 EPSPS), which confers resistance to glyphosate, the main herbicide worldwide used. The possible impact of genetically modified organisms ...
DOI:
10.1016/j.talanta.2021.122910

8. Effects of host vimentin on Eimeria tenella sporozoite invasion

Author:
Zhan Liu; Xiangfei Geng; Qiping Zhao; Shunhai Zhu; Hongyu Han; Yu Yu; Wenhao Huang; Yawen Yao; Bing Huang; Hui Dong
Source:
Parasites & vectors 2022 v.15 no.1 pp. 8
ISSN:
1756-3305
Subject:
polyclonal antibodies,   etc   ; Eimeria tenella; chickens; coccidiosis; financial economics; fluorescent antibody technique; industry; phosphorylation; proteomics; sporozoites; vimentin;     Show all 11 Subjects
Abstract:
... BACKGROUND: Chicken coccidiosis is a parasitic disease caused by Eimeria of Apicomplexa, which has caused great economic loss to the poultry breeding industry. Host vimentin is a key protein in the process of infection of many pathogens. In an earlier phosphorylation proteomics study, we found that the phosphorylation level of host vimentin was significantly regulated after Eimeria tenella sporozo ...
DOI:
10.1186/s13071-021-05107-4

9. Dermatan sulfate and chondroitin sulfate from Lophius litulon alleviate the allergy sensitized by major royal jelly protein 1

Author:
Xue Huang; Na Lin; Xinle Liang; Hong Zhang
Source:
Food & function 2022 v.13 no.2 pp. 587-595
ISSN:
2042-650X
Subject:
polyclonal antibodies,   etc   ; Lophius litulon; chondroitin sulfate; dermatan sulfate; food allergies; histamine; interleukin-4; royal jelly; splenocytes;     Show all 9 Subjects
Abstract:
... The objective of the present study was to explore the desensitization effect of dermatan sulfate (DS) and chondroitin sulfate (CS) from Lophius litulon (Ll) on mice sensitized by major royal jelly protein 1 (MRJP1). First, the affinity between six glycosaminoglycans and the MRJP1 polyclonal antibody was measured by the ELISA method. Lophius litulon dermatan sulfate (Ll DS) and Lophius litulon chon ...
DOI:
10.1039/d1fo03244e

10. Development of an indirect competitive enzyme linked immunosorbent assay for the quantitative detection of Mycoplasma hyopneumoniae during the vaccine production process

Author:
Yanna Wei; Thandeka Khoza; Yanfei Yu; Li Wang; Beibei Liu; Jia Wang; Lanxi Gan; Fei Hao; Guoqing Shao; Zhixin Feng; Qiyan Xiong
Source:
Journal of immunological methods 2022 v.500 pp. 113196
ISSN:
0022-1759
Subject:
polyclonal antibodies,   etc   ; Mycoplasma hyopneumoniae; antigens; color; enzymes; equations; inactivated vaccines; live vaccines; peptide elongation factors; regression analysis;     Show all 10 Subjects
Abstract:
... Inactivated Mycoplasma hyopneumoniae vaccine is used extensively to control M. hyopneumoniae infection worldwide. Quantification techniques are essential in the process of standardizing and validating vaccines. In this study, we developed and optimized an indirect competitive enzyme linked immunosorbent assay (ic-ELISA) for the rapid quantification of M. hyopneumoniae antigen during vaccine produc ...
DOI:
10.1016/j.jim.2021.113196

11. The function of Lmpt in Drosophila heart tissue

Author:
Meihua She; Jiawei Zhang; Tingting Jiang; Yurong Zhang; Yanjun Liu; Min Tang; Qun Zeng
Source:
Biochemical and biophysical research communications 2022 v.612 pp. 15-21
ISSN:
0006-291X
Subject:
polyclonal antibodies,   etc   ; CRISPR-Cas systems; Drosophila melanogaster; genetic models; heart; heart failure; homozygosity; muscle tissues; research; vertebrates;     Show all 10 Subjects
Abstract:
... Drosophila melanogaster, a classical genetic model organism, is widely used in the field of research on cardiac development and pathophysiological changes. Drosophila Lmpt, a LIM domain protein, is highly homologous to the vertebrate Fhl2. Fhl2 mutations cause heart failure, but the molecular mechanism is still unclear. Firstly, we prepared Lmpt polyclonal antibody and detected the expression of e ...
DOI:
10.1016/j.bbrc.2022.04.098

12. Disposable amperometric immunosensor with a dual monomers-based bioconjugate for granzyme B detection in blood and cancer progress monitoring of patients

Author:
Heru Agung Saputra; Jae Heun Chung; Seong Hoon Yoon; Kyeong-Deok Seo; Deog-Su Park; Yoon-Bo Shim
Source:
Biosensors & bioelectronics 2022 v.198 pp. 113846
ISSN:
0956-5663
Subject:
polyclonal antibodies,   etc   ; blood serum; detection limit; electrochemistry; humans; immunosensors; immunotherapy; lung neoplasms; monoclonal antibodies; neoplasm progression; patients;     Show all 11 Subjects
Abstract:
... A disposable amperometric biosensor with a dual monomers-based bioconjugate was developed for granzyme B (GzmB) detection and for monitoring of the cancer progression of patients before and after immunotherapy. The biosensor was fabricated by immobilizing a GzmB monoclonal antibody (Ab₁) on a poly3’-(2-aminopyrimidyl)-2,2’:5’,2’’-terthiophene/gold nanoparticle (pPATT/AuNP) layer. The bioconjugate ...
DOI:
10.1016/j.bios.2021.113846

13. Research Note: Development of a sandwich ELISA for determining plasma growth hormone concentrations in goose

Author:
R. Chen; R.H. Guo; M.M. Lei; H.X. Zhu; L.Y. Yan; Z.D. Shi
Source:
Poultry science 2022 v.101 no.3 pp. 101631
ISSN:
0032-5791
Subject:
polyclonal antibodies,   etc   ; absorbance; antigens; enzyme-linked immunosorbent assay; geese; logit analysis; metabolism; peroxidase; postnatal development; reference standards; somatotropin;     Show all 11 Subjects
Abstract:
... Growth hormone (GH) is required for normal postnatal development in poultry; however, no immunoassay exists to assess its levels in geese plasma, hindering the study of endocrine regulation in this species. We developed a sandwich ELISA to determine the GH concentrations in the plasma of geese. Recombinant goose GH was produced using a eukaryotic expression system and purified for use as the refer ...
DOI:
10.1016/j.psj.2021.101631

14. Fabrication of lateral flow immunoassay strip for rapid detection of acute hepatopancreatic necrosis disease

Author:
Ngoc-Diem Duong; Khai-Hoan Nguyen-Phuoc; Thuy-Dung Mai-Hoang; Kim-Yen Thi Do; Tuan-Binh Huynh; Nguyet-Thu Thi Nguyen; Thuoc Linh Tran; Hieu Tran-Van
Source:
3 Biotech 2022 v.12 no.10 pp. 243
ISSN:
2190-572X
Subject:
polyclonal antibodies,   etc   ; cross reaction; detection limit; disease detection; goats; gold; immunoassays; necrotizing hepatopancreatitis; rabbits; rapid methods; shrimp culture;     Show all 11 Subjects
Abstract:
... Acute hepatopancreatic necrosis disease (AHPND) is a contagious disease for the shrimp cultivation, thus early detection of disease is an unmet need. This present study documented for the first time a simple lateral flow immunoassay (LFIA) strip using polyclonal antibodies was created for the rapid detection both of PirAᵛᵖ and PirBᵛᵖ protein simultaneously. LFIA method based on the principle of sa ...
DOI:
10.1007/s13205-022-03311-2

15. Preparation of polyclonal antibody against phosphatidylethanolamine binding protein 1 recombinant protein and its functional verification in pulmonary hypertension syndrome in broilers

Author:
Qingqing Li; Yueming Gu; Xiaona Gao; Xiaoquan Guo; Cheng Huang; Pei Liu; Guoliang Hu; Guyue Li; Weile Fang; Wanrui Mai; Cong Wu; Zheng Xu; Feixiang Huang; Ping Liu
Source:
International journal of biological macromolecules 2022 v.213 pp. 19-26
ISSN:
0141-8130
Subject:
polyclonal antibodies,   etc   ; Western blotting; angiogenesis; ascites; fluorescent antibody technique; genetic vectors; hypertension; metastasis; neoplasms; phosphatidylethanolamines; pulmonary artery; recombinant proteins;     Show all 12 Subjects
Abstract:
... Pulmonary hypertension syndrome (PHS) is a disease that is difficult to overcome for fast-growing broilers. It causes pulmonary vascular remodeling and ascites in broilers. As a classical inhibitor of cancer metastasis, phosphatidylethanolamine binding protein 1 (PEBP1) regulates angiogenesis in the process of tumor metastasis through multiple signal pathways. However, whether PEBP1 can regulate p ...
DOI:
10.1016/j.ijbiomac.2022.05.159

16. Development and evaluation of nucleoprotein-based rapid detection test for Siniperca chuatsi rhabdovirus

Author:
Zhixin Chen; Nanxin Gong; Jingxuan Niu; Ruixian Xu; Huashan Yi; Yuzhu Song; Qinqin Han; Xueshan Xia; Ningqiu Li; Fugui Liu; Hongru Liang; Jinyang Zhang
Source:
Aquaculture 2022 v.546 pp. 737403
ISSN:
0044-8486
Subject:
polyclonal antibodies,   etc   ; Micropterus salmoides; Rhabdoviridae; Siniperca chuatsi; enzyme-linked immunosorbent assay; fish; genes; mortality; nucleoproteins; rapid methods; recombinant proteins; viruses;     Show all 12 Subjects
Abstract:
... Siniperca chuatsi rhabdovirus (SCRV) is a rod- or bullet-shaped negative-stranded RNA virus and a threat to mandarin fish farming. This virus naturally infects fish, such as S. chuatsi and Micropterus salmoides, among others. SCRV-infected fish have a 90% mortality rate. In this study, a recombinant SCRV-N protein and a polyclonal antibody were prepared by gene cloning, constructing the vector of ...
DOI:
10.1016/j.aquaculture.2021.737403

17. Indiscriminate SARS-CoV-2 multivariant detection using magnetic nanoparticle-based electrochemical immunosensing

Author:
Ceren Durmus; Simge Balaban Hanoglu; Duygu Harmanci; Hichem Moulahoum; Kerem Tok; Faezeh Ghorbanizamani; Serdar Sanli; Figen Zihnioglu; Serap Evran; Candan Cicek; Ruchan Sertoz; Bilgin Arda; Tuncay Goksel; Kutsal Turhan; Suna Timur
Source:
Talanta 2022 v.243 pp. 123356
ISSN:
0039-9140
Subject:
polyclonal antibodies,   etc   ; Severe acute respiratory syndrome coronavirus 2; calibration; detection limit; economic valuation; electrochemistry; humans; immunosensors; magnetism; mutation rate; pandemic; viruses;     Show all 12 Subjects
Abstract:
... The increasing mutation frequency of the SARS-CoV-2 virus and the emergence of successive variants have made correct diagnosis hard to perform. Developing efficient and accurate methods to diagnose infected patients is crucial to effectively mitigate the pandemic. Here, we developed an electrochemical immunosensor based on SARS-CoV-2 antibody cocktail-conjugated magnetic nanoparticles for the sens ...
DOI:
10.1016/j.talanta.2022.123356

18. Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Author:
Michaela Mrkvová; Richard Hančinský; Simona Grešíková; Šarlota Kaňuková; Ján Barilla; Miroslav Glasa; Pavol Hauptvogel; Ján Kraic; Daniel Mihálik
Source:
Viruses 2022 v.14 no.6 pp. -
ISSN:
1999-4915
Subject:
polyclonal antibodies,   etc   ; Tomato mosaic virus; coat proteins; cross reaction; diagnostic techniques; eggplants; immunoblotting; immunochemistry; oligopeptides; pepper; plant protection; rhizosphere; soil; tomatoes; viruses;     Show all 15 Subjects
Abstract:
... Plant viruses threaten agricultural production by reducing the yield, quality, and economical benefits. Tomato mosaic virus (ToMV) from the genus Tobamovirus causes serious losses in the quantity and quality of tomato production. The management of plant protection is very difficult, mainly due to the vector-less transmission of ToMV. Resistant breeding generally has low effectiveness. The most pra ...
DOI:
10.3390/v14061331

19. CCR4-NOT Complex 2—A Cofactor in Host Cell for Porcine Epidemic Diarrhea Virus Infection

Author:
Jieru Wang; Hailong Liu; Dongdong Yin; Mei Zhou; Lei Yin; Yuqing Yang; Zishi Guo; Xuehuai Shen; Yin Dai; Shaohua Shi; Shengsong Xie; Ruihong Zhao; Xueli Zhou; Xiaomiao Hu; Hongyan Hou; Chonglong Wang; Xiaocheng Pan
Source:
Genes 2022 v.13 no.9 pp. -
ISSN:
2073-4425
Subject:
polyclonal antibodies,   etc   ; Porcine epidemic diarrhea virus; host-pathogen relationships; industry; liquid chromatography; membrane proteins; plasma membrane; rabbits; surface plasmon resonance; swine; tandem mass spectrometry;     Show all 11 Subjects
Abstract:
... The porcine epidemic diarrhea virus (PEDV) has catastrophic impacts on the global pig industry. However, there is no consensus on the primary receptor associated with the PEDV invasion of host cells. An increasing number of studies have reported that PEDV invading host cells may require collaboration between multiple receptors and to better understand the virus-host interaction during PEDV entry, ...
DOI:
10.3390/genes13091504

20. An immunochromatographic test strip for on-site detection of Ralstonia solanacearum in tobacco leaves and soil

Author:
Xuehui Tian; Zichen Zhao; Huimeng Wang; Yuling Qin; Dongkun Wang; Yichi Li; Xiaoqiang Wang; Limin Wang
Source:
Analytical biochemistry 2022 v.641 pp. 114561
ISSN:
0003-2697
Subject:
polyclonal antibodies,   etc   ; Alternaria alternata; Ralstonia solanacearum; bacterial wilt; biochemistry; cross reaction; detection limit; disease control; immunoaffinity chromatography; leaf area; monoclonal antibodies; soil; tobacco; China;     Show all 14 Subjects
Abstract:
... Tobacco bacterial wilt is one of the most devastating soil-borne diseases in tobacco-producing regions worldwide. It is often responsible for significant economic losses during tobacco production. A rapid, specific, and high-throughput on-site detection method is important for plant disease management. In this study, monoclonal antibody 3H3 and polyclonal antibody 0344 specific for Ralstonia solan ...
DOI:
10.1016/j.ab.2022.114561