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You searched for: Subject "polygalacturonase" Remove constraint Subject: "polygalacturonase"
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1. Changes in the cell walls on fruit skin of Beurré D´Anjou pears (Pyrus communis L.) associated with sunburn injury

Author:
Nazarena Spera; Julia Ousset; Pedro M. Civello; Graciela M. Colavita
Source:
Scientia horticulturae 2023 v.307 pp. 111524
ISSN:
0304-4238
Subject:
polygalacturonase,   etc   ; Pyrus communis; cell walls; fractionation; fruits; harvest date; hemicellulose; lignin; pears; pectins; stress tolerance; sunburn; uronic acids; xyloglucan:xyloglucosyl transferase;     Show all 14 Subjects
Abstract:
... The purpose of this work was to evaluate the effects of sunburn on pear fruit skin at the structural, biochemical, and molecular levels. Pears (Pyrus communis L.) cv. Beurré D'Anjou were collected at the official commercial harvest time and classified into three groups based on levels of sublethal sunburn: no sunburn (S0), mild sunburn (S1), and moderate sunburn (S2). Skin anatomy was characterize ...
DOI:
10.1016/j.scienta.2022.111524

2. Degradation of water-soluble polysaccharides in pulp of litchi during storage

Author:
Zhongsuzhi Chen; Meiying He; Yijie Zhou; Xi Chen; Hong Zhu; Bao Yang; Yueming Jiang; Hongxia Qu
Source:
Food chemistry 2023 v.402 pp. 134289
ISSN:
0308-8146
Subject:
polygalacturonase,   etc   ; arabinogalactans; arabinose; depolymerization; energy; food chemistry; galactose; gene expression; mannose; molecular weight; pectate lyase; pectinesterase; pulp; storage time; water solubility;     Show all 15 Subjects
Abstract:
... Water-soluble polysaccharides (WSP) were extracted from the pulp of litchi. Its main component was identified as arabinogalactan. The dominant monosaccharide constituents were arabinose and galactose. Galactose and mannose accumulated at the end of storage. ATP, ADP and AMP levels declined with increasing pulp breakdown index. WSP depolymerized which was characterized by a decrease in its content ...
DOI:
10.1016/j.foodchem.2022.134289

3. Effects of postharvest calcium treatmenttreatment on the firmness of persimmon (Diospyros kaki) fruit based on a decline in WSP

Author:
Mengpei Liu; Rong Wang; Wenwen Sun; Weijuan Han; Ge Li; Wei Zong; Jianmin Fu
Source:
Scientia horticulturae 2023 v.307 pp. 111490
ISSN:
0304-4238
Subject:
polygalacturonase,   etc   ; Diospyros kaki; calcium; cell walls; climacteric fruits; enzyme activity; firmness; fruit quality; molecular weight; pectin lyase; pectinesterase; pectins; persimmons; vacuoles; water solubility;     Show all 15 Subjects
Abstract:
... Persimmon fruit is a typical respiratory climacteric fruit. Previous studies have found that water-soluble pectin (WSP) was closely related to fruit firmness, and calcium was involved in pectin bridging, which also affected fruit firmness. In this research, the effect of calcium treatment (2% CaCl₂, 30 min) on persimmon fruit firmness was studied from the perspective of WSP. Firmness of persimmon ...
DOI:
10.1016/j.scienta.2022.111490

4. Physiological and transcriptomic analysis reveals the postharvest ripening differences between “Nantianhuang” and “Brazilian” banana fruit

Author:
Jiao Chen; Yixing Li; Fenfang Li; Debao Yuan; Keqian Hong
Source:
Postharvest biology and technology 2023 v.195 pp. 112138
ISSN:
0925-5214
Subject:
polygalacturonase,   etc   ; Fusarium wilt; aminocyclopropanecarboxylate oxidase; bananas; cell respiration; climacteric fruits; cultivars; ethylene production; firmness; fruit quality; gene expression; industry; malondialdehyde; pectinesterase; starch; technology; transcriptome; transcriptomics;     Show all 18 Subjects
Abstract:
... Banana, as a typical climacteric fruit, undergoes a ripening process which impacts fruit quality. The Nantianhuang (NTH) banana fruit is a new cultivar resistant to Fusarium wilt, whose physiological characteristics and regulation mechanisms of fruit ripening are not clear. Ripening physiology and the related genes of the NTH fruit were compared with the Brazilian banana (BX) and investigated in t ...
DOI:
10.1016/j.postharvbio.2022.112138

5. Clarification of apple, grape and pear juices by co-immobilized amylase, pectinase and cellulase

Author:
Gul Ozyilmaz; Ecenur Gunay
Source:
Food chemistry 2023 v.398 pp. 133900
ISSN:
0308-8146
Subject:
polygalacturonase,   etc   ; amylases; antioxidants; apples; batch systems; computer software; endo-1,4-beta-glucanase; enzymatic treatment; experimental design; food chemistry; grapes; pears; response surface methodology; silica gel; temperature; turbidity;     Show all 16 Subjects
Abstract:
... In this study, the use of co-immobilized amylase, pectinase and cellulase enzymes for clarification of apple, grape and pear juices were investigated. Four different co-immobilization method was applied and the highest activity was observed for co-immobilized enzymes onto silica gel. The effect of the enzyme units used in the co-immobilization, reaction temperature and time on turbidity and reduci ...
DOI:
10.1016/j.foodchem.2022.133900

6. In situ ESEM using 3-D printed and adapted accessories to observe living plantlets and their interaction with enzyme and fungus

Author:
Romain Roulard; Michel Trentin; Valérie Lefebvre; Françoise Fournet; Ludivine Hocq; Jérôme Pelloux; Éric Husson; Christophe Pineau; Loïc Dupont; Arash Jamali
Source:
Micron 2022 v.153 pp. 103185
ISSN:
0968-4328
Subject:
polygalacturonase,   etc   ; Arabidopsis; Verticillium dahliae; flax; fungi; hyphae; plantlets;     Show all 7 Subjects
Abstract:
... This paper describes an innovative way of using environmental scanning electron microscopy (ESEM) and the development of a suitable accessory to perform in situ observation of living seedlings in the ESEM. We provide details on fabrication of an accessory that proved to be essential for such experiments but inexpensive and easy to build in the laboratory, and present our in situ observations of th ...
DOI:
10.1016/j.micron.2021.103185

7. Enzyme-assisted extraction of plant material – New functional aspects of the process on an example of Medicago sativa L.

Author:
Katarzyna Rafińska; Olga Wrona; Aneta Krakowska-Sieprawska; Justyna Walczak-Skierska; Anna Kiełbasa; Zbigniew Rafiński; Paweł Pomastowski; Mateusz Kolankowski; Bogusław Buszewski
Source:
Industrial crops and products 2022 v.187 pp. 115424
ISSN:
0926-6690
Subject:
polygalacturonase,   etc   ; Medicago sativa; enzymatic hydrolysis; flavonoids; hydrolysis; transmission electron microscopy;     Show all 6 Subjects
Abstract:
... Enzyme-assisted extraction of plant material is widely used method for isolation of biologically active compounds. Changes in the structure of the plant material occuring during enzymatic hydrolysis and their consequences for the extraction process of biological active compounds as well functional aspects of the quality of the obtained extracts are usually not considered. Therefore, the aim of the ...
DOI:
10.1016/j.indcrop.2022.115424

8. Host induced gene silencing of Sclerotinia sclerotiorum effector genes for the control of white mold

Author:
M.R. Maximiano; L.S. Santos; C. Santos; F.J.L. Aragão; S.C. Dias; O.L. Franco; A. Mehta
Source:
Biocatalysis and agricultural biotechnology 2022 v.40 pp. 102302
ISSN:
1878-8181
Subject:
polygalacturonase,   etc   ; Arabidopsis thaliana; Sclerotinia sclerotiorum; agricultural biotechnology; biocatalysis; calcium; genes; integrins;     Show all 8 Subjects
Abstract:
... Sclerotinia sclerotiorum (Lib.), the causal agent of white mold, is a necrotrophic fungus with worldwide distribution. This fungus can infect more than 600 agricultural crops, causing damage worth hundreds of millions of dollars annually. The control of white mold is usually performed using integrated management practices. Nevertheless, after the establishment of the disease, chemical compounds ne ...
DOI:
10.1016/j.bcab.2022.102302

9. Revealing methyl-esterification patterns of pectins by enzymatic fingerprinting: Beyond the degree of blockiness

Author:
Éva Jermendi; Martin Beukema; Marco A. van den Berg; Paul de Vos; Henk A. Schols
Source:
Carbohydrate polymers 2022 v.277 pp. 118813
ISSN:
0144-8617
Subject:
polygalacturonase,   etc   ; Aspergillus niger; Citrus; Kluyveromyces marxianus; enzymatic treatment; pectin lyase; pectins;     Show all 7 Subjects
Abstract:
... Citrus pectins were studied by enzymatic fingerprinting using a simultaneous enzyme treatment with endo-polygalacturonase (endo-PG) from Kluyveromyces fragilis and pectin lyase (PL) from Aspergillus niger to reveal the methyl-ester distribution patterns over the pectin backbone. Using HILIC-MS combined with HPAEC enabled the separation and identification of the diagnostic oligomers released. Struc ...
DOI:
10.1016/j.carbpol.2021.118813

10. The potential of subcritical water as a “green” method for the extraction and modification of pectin: A critical review

Author:
Somnath Basak; Uday S. Annapure
Source:
Food research international 2022 v.161 pp. 111849
ISSN:
0963-9969
Subject:
polygalacturonase,   etc   ; denaturation; food research; oligosaccharides; polygalacturonic acid; protein content; temperature; thermodynamics;     Show all 8 Subjects
Abstract:
... Subcritical water is a “green” method of extraction and modification of pectin being explored in recent times. While the conventional acid extraction degrades the side chains and produces homogalacturonan (HG)-rich pectic polysaccharides, subcritical water extraction preserved the hairy region, namely the rhamnogalacturonan-I (RG-I) region of the pectin. However, higher temperatures (usually great ...
DOI:
10.1016/j.foodres.2022.111849

11. Optimisation of Xylanase–Pectinase Cocktail Production with Bacillus amyloliquefaciens ADI2 Using a Low-Cost Substrate via Statistical Strategy

Author:
Muhammad Hariadi Nawawi; Khairul Izdihar Ismail; Norazliza Sa’ad; Rosfarizan Mohamad; Paridah Md Tahir; Ainun Zuriyati Asa’ari; Wan Zuhainis Saad
Source:
Fermentation 2022 v.8 no.3 pp. -
ISSN:
2311-5637
Subject:
polygalacturonase,   etc   ; Bacillus amyloliquefaciens; agitation; inoculum; models; pH; submerged fermentation; temperature; xylanases;     Show all 9 Subjects
Abstract:
... An effective statistical tool for increasing and boosting the production of xylanase and pectinase by Bacillus amyloliquefaciens ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 °C, pH of 8.38, inoculum size of 4% (w/v) and a ...
DOI:
10.3390/fermentation8030119

12. Comparison and Intercorrelation of Extraction Methods for Polyphenol Content and Antioxidant Capacity of Scab-Resistant Apple Cultivars

Author:
Mihály Orosz-Tóth; Andrea Nemes-Kun; Daniel A. Lowy; Ádám Csihon; Zsolt Sándor; Ida Kincses; Imre J. Holb
Source:
Agronomy 2022 v.12 no.2 pp. -
ISSN:
2073-4395
Subject:
polygalacturonase,   etc   ; acetone; agronomy; antioxidant activity; antioxidants; apples; control methods; cultivars; ethanol; methanol; polyphenols;     Show all 11 Subjects
Abstract:
... Polyphenols with antioxidant compounds represent an important group of bioactive substances in apple production. The objectives of this study were firstly to measure four parameters of antioxidant capacity (AC) and polyphenol content (AC-FRAP (Ferric Reducing Ability of Plasma), AC-DPPH (2,2-diphenyl-1-picrylhydrol), AC-TEAC (Trolox Equivalent Antioxidant Capacity) and total polyphenol content (TP ...
DOI:
10.3390/agronomy12020289

13. Effect of three kinds of biological pretreatments on substrate characteristics and sugar yield by enzymatic hydrolysis of Eichhornia crassipes biomass

Author:
Zejin Guo; Qing Yang; Wenbing Zhou; Naidong Xiao; Jianbo Cai
Source:
Bioresource technology reports 2022 v.17 pp. 100983
ISSN:
2589-014X
Subject:
polygalacturonase,   etc   ; Eichhornia crassipes; Phanerochaete chrysosporium; biomass; cellulose; degumming; enzymatic hydrolysis; fungi; lignin; saccharification;     Show all 10 Subjects
Abstract:
... This study compared the enzymatic saccharification of Eichhornia crassipes (EC) biomass after three kinds of biological pretreatments, i.e. commercial fungus Phanerochaete chrysosporium (P. chrysosporium), self-screened degumming bacteria CTS-BQ1, and commercial pectinase pretreatment. The chemical composition and sugar yield under enzymatic hydrolysis of EC substrate were determined. The results ...
DOI:
10.1016/j.biteb.2022.100983

14. Enzyme hydrolyzed bael fruit liquefaction and its kinetic study

Author:
Rishab Dhar; Snehasis Chakraborty
Source:
Food bioscience 2022 v.47 pp. 101779
ISSN:
2212-4292
Subject:
polygalacturonase,   etc   ; Aegle marmelos; enzymatic hydrolysis; enzyme activity; juices; liquefaction; nutrient content; pomace; temperature; yields;     Show all 10 Subjects
Abstract:
... The study aims to explore the mechanism and optimize the process of enzyme hydrolyzed bael fruit (Aegle marmelos) liquefaction using two different enzymes: pectinase and macerozyme r-10 (enzyme mixture). A full factorial design was applied for the factors with 0.05–1.5% (w/w) enzyme concentration (EC), 30–60 °C temperature, and incubation time of 30–240 min for pectinase and 60–360 min for maceroz ...
DOI:
10.1016/j.fbio.2022.101779

15. A sustainable approach for cotton bioscouring: reuse of the pectate lyase containing treatment bath

Author:
Bruna Lyra Colombi; Marlon David Palozi; Rita de Cássia Siqueira Curto Valle; Jürgen Andreaus; Manuel José Lis Arias; José Alexandre Borges Valle
Source:
Bioprocess and biosystems engineering 2022 v.45 no.8 pp. 1391-1405
ISSN:
1615-7591
Subject:
polygalacturonase,   etc   ; bioscouring; cotton fabric; dyeing; industrial applications; pH; pectate lyase; pectins; textile industry; wastewater;     Show all 10 Subjects
Abstract:
... Enzymatic scouring of cotton has established itself (slowly) as a green alternative to alkaline scouring in the textile industry, mostly due to more environmentally friendly processing at lower pH and temperatures and its less aggressive action on the cotton fibers. However, among other limitations, enzyme costs have contributed to impeding its wide acceptance and use. For the first time, in this ...
DOI:
10.1007/s00449-022-02753-5

16. Inoculation conditions improved the pectinase productivity in Aspergillus niger LB-02-SF solid-state cultivation

Author:
Caroline Reginatto; Gabriela Posso dos Santos; Kimberly Costa Ramos; Analia Borges Folle; Bruna Campos de Souza; Lenara Meneghel; Sabrina Carra; Tomas Augusto Polidoro; Mauricio Moura da Silveira; Eunice Valduga; Eloane Malvessi
Source:
Biocatalysis and agricultural biotechnology 2022 v.42 pp. 102354
ISSN:
1878-8181
Subject:
polygalacturonase,   etc   ; Aspergillus niger; agricultural biotechnology; biocatalysis; bioreactors; fermentation; inoculum; liquids; solid state culture; spores;     Show all 10 Subjects
Abstract:
... In this work, the pectinase production by Aspergillus niger LB-02-SF was evaluated in relation to the inoculation conditions of solid-state cultivations carried out in Becher flasks and in rotating drum bioreactor. From the inoculation by spore suspension in the concentration of 7 × 10⁷ spore/100 g of wet medium (gwm), which is standard inoculum condition, were evaluated: different concentrations ...
DOI:
10.1016/j.bcab.2022.102354

17. Relationship of the methanol production, pectin and pectinase activity during apple wine fermentation and aging

Author:
Yingying Han; Jinhua Du
Source:
Food research international 2022 v.159 pp. 111645
ISSN:
0963-9969
Subject:
polygalacturonase,   etc   ; apple juice; apples; fermentation; food research; methanol; molecular weight; pectin lyase; pectinesterase; pectins; wines;     Show all 11 Subjects
Abstract:
... Understanding pectin structure and pectinase activity was important to control methanol content in apple wine. Therefore, this study compared inoculated fermentation (I), spontaneous fermentation (S) and inoculated fermentation combined with CaCl₂ treatment (I & CaCl₂) to explore their differences in methanol production, pectin peak molecular weight (Mp), and the activities of pectin methyl estera ...
DOI:
10.1016/j.foodres.2022.111645

18. Covalent Immobilization of Chondrostereum purpureum Endopolygalacturonase on Ferromagnetic Nanoparticles: Catalytic Properties and Biotechnological Application

Author:
Sibeli Carli; Jose Carlos Santos Salgado; Luana Parras Meleiro; Richard John Ward
Source:
Applied biochemistry and biotechnology 2022 v.194 no.2 pp. 848-861
ISSN:
0273-2289
Subject:
polygalacturonase,   etc   ; Chondrostereum purpureum; Komagataella pastoris; biotechnology; chitosan; ferromagnetism; immobilized enzymes; magnetic fields; synergism; thermal stability;     Show all 10 Subjects
Abstract:
... Pectinases are widely used in a variety of industrial processes. However, their application is limited by low catalytic processivity, reduced stability, high cost, and poor re-use compatibility. These drawbacks may be overcome by enzyme immobilization with ferromagnetic nanoparticles, which are easily recovered by a magnetic field. In this work, an endopolygalacturonase from Chondrostereum purpure ...
DOI:
10.1007/s12010-021-03688-5

19. Optimization of enzyme aided pigment extraction from pumpkin (Cucurbita maxima Duch) using response surface methodology

Author:
Anu Sharma; Dalbir Singh Sogi
Source:
Journal of food measurement & characterization 2022 v.16 no.2 pp. 1184-1194
ISSN:
2193-4126
Subject:
polygalacturonase,   etc   ; cellulose; mathematical models; measurement; nutritive value; pH; pumpkins; regression analysis; response surface methodology; temperature;     Show all 10 Subjects
Abstract:
... Pumpkin’s bio-pigment is a natural source to improve the appearance of food products along with nutritional value. Present work aimed at optimizing the carotenoids extraction from pumpkin with enzymatic pre-treatment. A central composite design with four independent variables-cellulose/pectinase ratio (0.25–1.91w/w); Incubation temperature (35–55 °C); Incubation time (30–150 min); pH (4–6) gave ca ...
DOI:
10.1007/s11694-021-01246-5

20. Statistical Optimization of Process Parameters for Polygalacturonase Production By Bacillus subtilis KP10B

Author:
Kavisha K. Patidar; Swati A. Peshwe
Source:
Applied biological research 2022 v.24 no.2 pp. 219-225
ISSN:
0974-4517
Subject:
polygalacturonase,   etc   ; Bacillus subtilis; clarification (processing); cost effectiveness; fermentation; fruit juices; industry; research; temperature; wastewater treatment;     Show all 10 Subjects
Abstract:
... In present study the statistical optimization of process parameters for costeffective production of polygalacturonase (PGase) by Bacillus subtilis was examined by using Plackett-Burman design (PBD) and central composite design (CCD). Of the 9 factors, temperature, CaCO₃ and KH₂PO₄ were found significant by PBD and were further optimized by CCD. After optimization, maximal PGase activity (272.87 Um ...
DOI:
10.5958/0974-4517.2022.00030.1