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quantitative polymerasechainreaction, etc ; Clostridium; antibiotic resistance; biotransformation; food safety; manure amendments; manure spreading; methylation; multiple drug resistance; paddies; paddy soils; pollution; risk; China; Show all 14 Subjects
Abstract:
... Paddy soils are potential hotspots of combined contamination with arsenic (As) and antibiotics, which may induce co-selection of antibiotic resistance genes (ARGs) and As biotransformation genes (ABGs), resulting in dissemination of antimicrobial resistance and modification in As biogeochemical cycling. So far, little information is available for these co-selection processes and specific patterns ...
reverse transcriptase polymerasechainreaction, etc ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibodies; biosensors; drug development; human health; mutation; pandemic; rapid methods; viruses; Show all 11 Subjects
Abstract:
... Currently, the coronavirus disease 2019 (COVID-19) pandemic is ravaging the world, causing serious crisis in economy and human health. The top priority is the detection and drug development of the novel coronavirus. The gold standard for real-time diagnosis of coronavirus disease is the reverse transcription-polymerase chain reaction (RT-PCR), which is usually operatively complex and time-consumin ...
quantitative polymerasechainreaction, etc ; Danio rerio; anilinopyrimidine fungicides; aryl hydrocarbon receptors; developmental toxicity; fruit growing; fungi; genes; light intensity; mepanipyrim; opsin; phototransduction; transcriptomics; wavelengths; China; Show all 15 Subjects
Abstract:
... Mepanipyrim, an anilinopyrimidine fungicide, has been extensively used to prevent fungal diseases in fruit culture. Currently, research on mepanipyrim-induced toxicity in organisms is still very scarce, especially visual developmental toxicity. Here, zebrafish larvae were employed to investigate mepanipyrim-induced visual developmental toxicity. Intense light and monochromatic light stimuli-evoked ...
quantitative polymerasechainreaction, etc ; biosensors; biotechnology; humans; serology; Show all 5 Subjects
Abstract:
... Cancer is undoubtedly one of the major human challenges worldwide. A number of pathogenic bacteria are deemed to be potentially associated with the disease. Accordingly, accurate and specific identification of cancer-associated bacteria can play an important role in cancer control and prevention. A variety of conventional methods such as culture, serology, and molecular-based methods as well as PC ...
polymerasechainreaction, etc ; bulk milk; dairy cattle; Argentina; Show all 4 Subjects
Abstract:
... There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M.bovis and M.canadense and to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to a diagnostic laboratory. Of a total of 154 ...
polymerasechainreaction, etc ; genome; nucleotide sequences; pathogens; poultry; Show all 5 Subjects
Abstract:
... PCR-based assays have become the benchmark for detecting pathogens of poultry and other livestock; however, these techniques are limited in their ability to detect multiple infecting agents, provide limited genetic information on the pathogen, and, for RNA viruses, must be reviewed frequently to assure high sensitivity and specificity. In contrast, untargeted, high-throughput sequencing can rapidl ...
quantitative polymerasechainreaction, etc ; microscopy; vesicular arbuscular mycorrhizae; Show all 3 Subjects
Abstract:
... Mycorrhizal colonization of roots is traditionally evaluated by empirical methods, such as root microscopy. We compared this method with data from using a real time PCR technique, and determined the correlation between methods, indicating particularities of a promising system for a quick and accurate molecular diagnostic of arbuscular mycorrhization. ...
Bart T. L. H. van de Vossenberg; Naomi te Braak; Tim Warbroek; Johan P. Meffert; Karin H. M. Rosendahl‐Peters; Marga P. E. van Gent‐Pelzer; Patricia C. J. van Rijswick
quantitative polymerasechainreaction, etc ; Synchytrium endobioticum; potatoes; soil; Show all 4 Subjects
Abstract:
... Plots infested with Synchytrium endobioticum, the causal agent of potato wart disease, are scheduled, resulting in prohibition of potato cultivation of and cultivation of plants for planting. On account of robust resting spores that are present in infested soils, plots remain scheduled for 20 years. After this period, plots are intensively sampled and the presence of resting spores in soil is dete ...
quantitative polymerasechainreaction, etc ; DNA; automation; fluorescence; oligonucleotides; Show all 5 Subjects
Abstract:
... The quantitative polymerase chain reaction (qPCR) with detection of duplex DNA yield by intercalator fluorescence is a common and essential technique in nucleic acid analysis. We encountered unexpected results when applying standard qPCR methods to the quantitation of random DNA libraries flanked by regions of fixed sequence, a configuration essential for in vitro selection experiments. Here we de ...
polymerasechainreaction, etc ; Astroviridae; diagnostic techniques; histology; swine; Show all 5 Subjects
Abstract:
... Porcine astroviruses (PoAstVs) have been reported globally and are divided into at least five distinct lineages (PoAstV1‐PoAsV5). The primary objective of this review was to summarize the scientific literature about the frequency of detection, associated clinical presentations and type of samples and diagnostic tools used for the detection of porcine astroviruses. The secondary objective was to su ...
... The increase of CO₂ by anthropogenic activities leads to a decrease of pH in the ocean surface due to ocean acidification (OA) process. Generally, OA not only reduces the rate of calcification in marine environments but also affects various physiological activities, especially in calcifiers, including edible oysters. Quantitative real-time PCR (qRT-PCR) is often used to detect gene expression in r ...
... Species A rotavirus (RVA) is a major viral pathogen causing diarrhea in suckling piglets. Studies on its genetic heterogeneity have implications for vaccine efficacy in the field. In this study, fecal samples (n = 866) from diarrheic piglets younger than 28 days were analyzed over a two-year period (2018–2019). Samples were submitted from 426 farms located in 36 provinces throughout Spain and were ...
quantitative polymerasechainreaction; reverse transcriptase polymerasechainreaction, etc ; Adiantum; apomixis; biotechnology; ferns and fern allies; gametophytes; gene expression; genes; gibberellins; sexual reproduction; sucrose; Show all 12 Subjects
Abstract:
... The molecular mechanism for development and reproduction of fern gametophytes has received wide attention for their various patterns of sexual organ differentiation. Stable reference genes could normalize the data for gene expression analysis using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Selecting appropriate reference genes would facilitate the gene funct ...
polymerasechainreaction, etc ; Escherichia coli; antigens; antiserum; flagellin; genes; Show all 6 Subjects
Abstract:
... Escherichia coli has traditionally been serotyped using antisera against the O and H antigens. However, a proportion of E. coli isolates are nonmotile and, in addition, some isolates do not react with the currently available H-typing sera. Alternative molecular methods have been developed based on the detection of genes encoding for H antigens. In this study, we studied 13 serologically nontypable ...
quantitative polymerasechainreaction, etc ; DNA; analytical chemistry; biopharmaceuticals; lipids; Show all 5 Subjects
Abstract:
... Lipid nanoparticles serve as drug delivery vehicles for biopharmaceutical products. The lipid membrane shields internal nucleic-acid drug cargo from enzymatic degradation and facilitates cellular uptake of the drug. However, existing methods to assess drug loading within liposomes are limited to averaged bulk measurements, which obscures heterogeneity of the biopharmaceutical formulation. This rep ...
quantitative polymerasechainreaction, etc ; Batrachochytrium dendrobatidis; Pelophylax; surveys; wildlife; Show all 5 Subjects
Abstract:
... We used quantitative PCR to detect Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bsal) in 135 samples of Pelophylax esculentus complex water frogs from 41 Latvian populations. We detected Bd in 18 populations of water frogs. None of the samples was positive for Bsal. ...
polymerasechainreaction, etc ; Human gammaherpesvirus 4; biosensors; models; plasmids; Show all 5 Subjects
Abstract:
... For digital polymerase chain reaction (PCR), data classification is always a crucial task. The dynamic real-time amplification process information of each partition is always ignored in typical digital PCR analysis, which can easily lead to inaccurate outcomes. In this work, an integrated device that offers real-time chip-based digital PCR analysis was established. In addition, an enhanced process ...
quantitative polymerasechainreaction, etc ; cattle; fluorescence; gene expression; genes; Show all 5 Subjects
Abstract:
... The stability of internal reference genes is crucial to the reliability of gene expression results using real-time fluorescence quantitative PCR (qRT-PCR). Inappropriate reference genes may lead to inaccurate results or even wrong conclusions. This study aims to identify stable reference genes for analyzing the expression of proliferation-related and differentiation-inducing genes in bovine primar ...
polymerasechainreaction, etc ; endometrium; gene expression; interleukin-10; pregnancy; Show all 5 Subjects
Abstract:
... This study aimed to determine the differences in gene expression between mononuclear cells derived from peripheral blood and endometrium during pregnancy in cattle and to determine the proportion of mononuclear cells in the endometrium of pregnant and diestrous cows. Endometrial tissue and peripheral blood were collected from Day 34±2 pregnant cows, and mononuclear cell populations were quant ...
quantitative polymerasechainreaction, etc ; commercialization; droplets; fluorescence; genes; soybeans; Show all 6 Subjects
Abstract:
... The presence of genetically modified organisms (GMOs) in food and feed products is regulated in many countries. Various countries have issued labeling threshold levels to supervise GMO commercialization, and efficient detection methods to quantify GMO contents are critical to meet labeling regulations. The aim of this study was to develop event-specific methods to measure the event-specific/lectin ...