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You searched for: Subject "polymerase chain reaction" Remove constraint Subject: "polymerase chain reaction"
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1. Co-occurrence of genes for antibiotic resistance and arsenic biotransformation in paddy soils

Author:
Huiling Cui; Dong Zhu; Longjun Ding; Yifei Wang; Jianqiang Su; Guilan Duan; Yongguan Zhu
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 701-711
ISSN:
1001-0742
Subject:
quantitative polymerase chain reaction,   etc   ; Clostridium; antibiotic resistance; biotransformation; food safety; manure amendments; manure spreading; methylation; multiple drug resistance; paddies; paddy soils; pollution; risk; China;     Show all 14 Subjects
Abstract:
... Paddy soils are potential hotspots of combined contamination with arsenic (As) and antibiotics, which may induce co-selection of antibiotic resistance genes (ARGs) and As biotransformation genes (ABGs), resulting in dissemination of antimicrobial resistance and modification in As biogeochemical cycling. So far, little information is available for these co-selection processes and specific patterns ...
DOI:
10.1016/j.jes.2022.02.027

2. Research progress of biosensors for detection of SARS-CoV-2 variants based on ACE2

Author:
Hongshuai Wei; Congcong Zhang; Xin Du; Zhenguo Zhang
Source:
Talanta 2023 v.251 pp. 123813
ISSN:
0039-9140
Subject:
reverse transcriptase polymerase chain reaction,   etc   ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibodies; biosensors; drug development; human health; mutation; pandemic; rapid methods; viruses;     Show all 11 Subjects
Abstract:
... Currently, the coronavirus disease 2019 (COVID-19) pandemic is ravaging the world, causing serious crisis in economy and human health. The top priority is the detection and drug development of the novel coronavirus. The gold standard for real-time diagnosis of coronavirus disease is the reverse transcription-polymerase chain reaction (RT-PCR), which is usually operatively complex and time-consumin ...
DOI:
10.1016/j.talanta.2022.123813

3. Mepanipyrim induces visual developmental toxicity and vision-guided behavioral alteration in zebrafish larvae

Author:
Chao Shen; Yimei Cai; Jialing Li; Chengyong He; Zhenghong Zuo
Source:
Journal of environmental sciences (China) 2023 v.124 pp. 76-88
ISSN:
1001-0742
Subject:
quantitative polymerase chain reaction,   etc   ; Danio rerio; anilinopyrimidine fungicides; aryl hydrocarbon receptors; developmental toxicity; fruit growing; fungi; genes; light intensity; mepanipyrim; opsin; phototransduction; transcriptomics; wavelengths; China;     Show all 15 Subjects
Abstract:
... Mepanipyrim, an anilinopyrimidine fungicide, has been extensively used to prevent fungal diseases in fruit culture. Currently, research on mepanipyrim-induced toxicity in organisms is still very scarce, especially visual developmental toxicity. Here, zebrafish larvae were employed to investigate mepanipyrim-induced visual developmental toxicity. Intense light and monochromatic light stimuli-evoked ...
DOI:
10.1016/j.jes.2021.11.008

4. Biosensors, modern technology for the detection of cancer-associated bacteria

Author:
Ahmad Mobed; Shirin Malehmir; Ali Ahmad alipour; Yasaman Azizimoghaddam; Hediyeh Saghi Sarabi; Farhood Ghazi
Source:
Biotechnology letters 2022 v.44 no.5-6 pp. 683-701
ISSN:
0141-5492
Subject:
quantitative polymerase chain reaction,   etc   ; biosensors; biotechnology; humans; serology;     Show all 5 Subjects
Abstract:
... Cancer is undoubtedly one of the major human challenges worldwide. A number of pathogenic bacteria are deemed to be potentially associated with the disease. Accordingly, accurate and specific identification of cancer-associated bacteria can play an important role in cancer control and prevention. A variety of conventional methods such as culture, serology, and molecular-based methods as well as PC ...
DOI:
10.1007/s10529-022-03257-8

5. Detection by multiplex PCR of Mycoplasma species associated with dairy cattle in Argentina

Author:
Verónica E. Neder; Ariel F. Amadio; Luis F. Calvinho
Source:
Revista argentina microbiología 2022 v.54 no.2 pp. 158-161
ISSN:
0325-7541
Subject:
polymerase chain reaction,   etc   ; bulk milk; dairy cattle; Argentina;     Show all 4 Subjects
Abstract:
... There is scarce information about the frequency and epidemiological and clinical features associated with the presence of Mycoplasma spp. in Argentine dairy herds. The objectives of this study were to develop a multiplex PCR for identifying M.bovis and M.canadense and to describe the frequency of Mycoplasma spp. isolated from clinical samples submitted to a diagnostic laboratory. Of a total of 154 ...
DOI:
10.1016/j.ram.2021.07.001

6. Non-target RNA depletion strategy to improve sensitivity of next-generation sequencing for the detection of RNA viruses in poultry

Author:
D. Joshua Parris; Henry Kariithi; David L. Suarez
Source:
Journal of veterinary diagnostic investigation 2022 v.34 no.4 pp. 638-645
ISSN:
1943-4936
Subject:
polymerase chain reaction,   etc   ; genome; nucleotide sequences; pathogens; poultry;     Show all 5 Subjects
Abstract:
... PCR-based assays have become the benchmark for detecting pathogens of poultry and other livestock; however, these techniques are limited in their ability to detect multiple infecting agents, provide limited genetic information on the pathogen, and, for RNA viruses, must be reviewed frequently to assure high sensitivity and specificity. In contrast, untargeted, high-throughput sequencing can rapidl ...
Handle:
10113/7822904
DOI:
10.1177/10406387221102430

7. Experimental validation under controlled conditions of real time PCR to quantify arbuscular mycorrhizal colonization in root

Author:
Bruna Arruda; Yasmin Florentino Rodrigues; Wilfrand Ferney Bejarano Herrera; Agnès Robin; Simone Raposo Cotta; Fernando Dini Andreote
Source:
Journal of microbiological methods 2022 v.192 pp. 106382
ISSN:
0167-7012
Subject:
quantitative polymerase chain reaction,   etc   ; microscopy; vesicular arbuscular mycorrhizae;     Show all 3 Subjects
Abstract:
... Mycorrhizal colonization of roots is traditionally evaluated by empirical methods, such as root microscopy. We compared this method with data from using a real time PCR technique, and determined the correlation between methods, indicating particularities of a promising system for a quick and accurate molecular diagnostic of arbuscular mycorrhization. ...
DOI:
10.1016/j.mimet.2021.106382

8. Comparison of real‐time PCR tests for the detection of Synchytrium endobioticum resting spores in soil and their potential application in descheduling of previously infested plots

Author:
Bart T. L. H. van de Vossenberg; Naomi te Braak; Tim Warbroek; Johan P. Meffert; Karin H. M. Rosendahl‐Peters; Marga P. E. van Gent‐Pelzer; Patricia C. J. van Rijswick
Source:
Bulletin OEPP 2022 v.52 no.1 pp. 154-163
ISSN:
0250-8052
Subject:
quantitative polymerase chain reaction,   etc   ; Synchytrium endobioticum; potatoes; soil;     Show all 4 Subjects
Abstract:
... Plots infested with Synchytrium endobioticum, the causal agent of potato wart disease, are scheduled, resulting in prohibition of potato cultivation of and cultivation of plants for planting. On account of robust resting spores that are present in infested soils, plots remain scheduled for 20 years. After this period, plots are intensively sampled and the presence of resting spores in soil is dete ...
DOI:
10.1111/epp.12813

9. Optimized quantitative PCR analysis of random DNA aptamer libraries

Author:
Keenan Pearson; Caroline Doherty; Drason Zhang; Nicole A. Becker; L. James Maher
Source:
Analytical biochemistry 2022 v.650 pp. 114712
ISSN:
0003-2697
Subject:
quantitative polymerase chain reaction,   etc   ; DNA; automation; fluorescence; oligonucleotides;     Show all 5 Subjects
Abstract:
... The quantitative polymerase chain reaction (qPCR) with detection of duplex DNA yield by intercalator fluorescence is a common and essential technique in nucleic acid analysis. We encountered unexpected results when applying standard qPCR methods to the quantitation of random DNA libraries flanked by regions of fixed sequence, a configuration essential for in vitro selection experiments. Here we de ...
DOI:
10.1016/j.ab.2022.114712

10. Scoping review on the epidemiology, diagnostics and clinical significance of porcine astroviruses

Author:
Gaurav Rawal; Daniel C. L. Linhares
Source:
Transboundary and emerging diseases 2022 v.69 no.3 pp. 974-985
ISSN:
1865-1674
Subject:
polymerase chain reaction,   etc   ; Astroviridae; diagnostic techniques; histology; swine;     Show all 5 Subjects
Abstract:
... Porcine astroviruses (PoAstVs) have been reported globally and are divided into at least five distinct lineages (PoAstV1‐PoAsV5). The primary objective of this review was to summarize the scientific literature about the frequency of detection, associated clinical presentations and type of samples and diagnostic tools used for the detection of porcine astroviruses. The secondary objective was to su ...
DOI:
10.1111/tbed.14123

11. Internal controls for quantitative RT-PCR analysis of gene expression in response to ocean acidification in edible oysters

Author:
Xin Dang; Zohaib Noor; Yuan-Qiu He; Yong-Kian Lim; Yang Zhang; Zi-Niu Yu; Vengatesen Thiyagarajan
Source:
Journal of experimental marine biology and ecology 2022 v.548 pp. 151683
ISSN:
0022-0981
Subject:
quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction,   etc   ; Crassostrea hongkongensis; calcification; carbon dioxide; computer software; ecology; gene expression; ocean acidification; pH; plankton;     Show all 11 Subjects
Abstract:
... The increase of CO₂ by anthropogenic activities leads to a decrease of pH in the ocean surface due to ocean acidification (OA) process. Generally, OA not only reduces the rate of calcification in marine environments but also affects various physiological activities, especially in calcifiers, including edible oysters. Quantitative real-time PCR (qRT-PCR) is often used to detect gene expression in r ...
DOI:
10.1016/j.jembe.2021.151683

12. Occurrence of Rotavirus A Genotypes and Other Enteric Pathogens in Diarrheic Suckling Piglets from Spanish Swine Farms

Author:
Luis V. Monteagudo; Alfredo A. Benito; Sofía Lázaro-Gaspar; José L. Arnal; Desirée Martin-Jurado; Rut Menjon; Joaquín Quílez
Source:
Animals 2022 v.12 no.3 pp. -
ISSN:
2076-2615
Subject:
quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction,   etc   ; Clostridium; Porcine epidemic diarrhea virus; Rotavirus A; amino acids; diarrhea; epitopes; genetic heterogeneity; vaccines; Spain;     Show all 11 Subjects
Abstract:
... Species A rotavirus (RVA) is a major viral pathogen causing diarrhea in suckling piglets. Studies on its genetic heterogeneity have implications for vaccine efficacy in the field. In this study, fecal samples (n = 866) from diarrheic piglets younger than 28 days were analyzed over a two-year period (2018–2019). Samples were submitted from 426 farms located in 36 provinces throughout Spain and were ...
DOI:
10.3390/ani12030251

13. Identification and validation of suitable reference genes for quantitative real-time PCR studies in Adiantum reniforme var. sinense

Author:
Qi Fu; Lin Xiang; Kai-ge Zhao; Long-qing Chen
Source:
Journal of plant biochemistry and biotechnology 2022 v.31 no.3 pp. 678-683
ISSN:
0971-7811
Subject:
quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction,   etc   ; Adiantum; apomixis; biotechnology; ferns and fern allies; gametophytes; gene expression; genes; gibberellins; sexual reproduction; sucrose;     Show all 12 Subjects
Abstract:
... The molecular mechanism for development and reproduction of fern gametophytes has received wide attention for their various patterns of sexual organ differentiation. Stable reference genes could normalize the data for gene expression analysis using real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Selecting appropriate reference genes would facilitate the gene funct ...
DOI:
10.1007/s13562-021-00763-0

14. Identification of Two New Sequences of Flagellin-Encoding Gene in Escherichia coli Using PCR and Sequencing-Based Methods

Author:
Ariel Diego Rogé; Ana Beatriz Celi Castillo; Claudia Andrea van der Ploeg; Ximena Luciana Bordagorría; Valeria M. Padín; Susana Beatriz Bruno
Source:
Foodborne pathogens & disease 2022 v.19 no.1 pp. 31-35
ISSN:
1556-7125
Subject:
polymerase chain reaction,   etc   ; Escherichia coli; antigens; antiserum; flagellin; genes;     Show all 6 Subjects
Abstract:
... Escherichia coli has traditionally been serotyped using antisera against the O and H antigens. However, a proportion of E. coli isolates are nonmotile and, in addition, some isolates do not react with the currently available H-typing sera. Alternative molecular methods have been developed based on the detection of genes encoding for H antigens. In this study, we studied 13 serologically nontypable ...
DOI:
10.1089/fpd.2021.0033

15. Microfluidic Digital Quantitative PCR to Measure Internal Cargo of Individual Liposomes

Author:
Bailey F. McCarthy Riley; Hao T. Mai; Thomas H. Linz
Source:
Analytical chemistry 2022 v.94 no.20 pp. 7433-7441
ISSN:
1520-6882
Subject:
quantitative polymerase chain reaction,   etc   ; DNA; analytical chemistry; biopharmaceuticals; lipids;     Show all 5 Subjects
Abstract:
... Lipid nanoparticles serve as drug delivery vehicles for biopharmaceutical products. The lipid membrane shields internal nucleic-acid drug cargo from enzymatic degradation and facilitates cellular uptake of the drug. However, existing methods to assess drug loading within liposomes are limited to averaged bulk measurements, which obscures heterogeneity of the biopharmaceutical formulation. This rep ...
DOI:
10.1021/acs.analchem.2c01232

16. Survey for Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans in Latvian Water Frogs

Author:
Alena A. Kulikova; Aija Pupina; Mihails Pupins; Andris Čeirāns; Vojtech Baláž
Source:
Journal of wildlife diseases 2022 v.58 no.2 pp. 440-444
ISSN:
0090-3558
Subject:
quantitative polymerase chain reaction,   etc   ; Batrachochytrium dendrobatidis; Pelophylax; surveys; wildlife;     Show all 5 Subjects
Abstract:
... We used quantitative PCR to detect Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bsal) in 135 samples of Pelophylax esculentus complex water frogs from 41 Latvian populations. We detected Bd in 18 populations of water frogs. None of the samples was positive for Bsal. ...
DOI:
10.7589/JWD-D-21-00082

17. The development of real-time digital PCR technology using an improved data classification method

Author:
Jia Yao; Yuanyuan Luo; Zhiqi Zhang; Jinze Li; Chuanyu Li; Chao Li; Zhen Guo; Lirong Wang; Wei Zhang; Heming Zhao; Lianqun Zhou
Source:
Biosensors & bioelectronics 2022 v.199 pp. 113873
ISSN:
0956-5663
Subject:
polymerase chain reaction,   etc   ; Human gammaherpesvirus 4; biosensors; models; plasmids;     Show all 5 Subjects
Abstract:
... For digital polymerase chain reaction (PCR), data classification is always a crucial task. The dynamic real-time amplification process information of each partition is always ignored in typical digital PCR analysis, which can easily lead to inaccurate outcomes. In this work, an integrated device that offers real-time chip-based digital PCR analysis was established. In addition, an enhanced process ...
DOI:
10.1016/j.bios.2021.113873

18. Analysis of stability of reference genes for qPCR in bovine preadipocytes during proliferation and differentiation in vitro

Author:
Guo-Hua Wang; Si-Hu Wang; Wen-Zhen Zhang; Cheng-Cheng Liang; Gong Cheng; Xiao-Yu Wang; Yu Zhang; Lin-Sen Zan
Source:
Gene 2022 v.830 pp. 146502
ISSN:
0378-1119
Subject:
quantitative polymerase chain reaction,   etc   ; cattle; fluorescence; gene expression; genes;     Show all 5 Subjects
Abstract:
... The stability of internal reference genes is crucial to the reliability of gene expression results using real-time fluorescence quantitative PCR (qRT-PCR). Inappropriate reference genes may lead to inaccurate results or even wrong conclusions. This study aims to identify stable reference genes for analyzing the expression of proliferation-related and differentiation-inducing genes in bovine primar ...
DOI:
10.1016/j.gene.2022.146502

19. Changes in mononuclear immune cells during bovine pregnancy

Author:
Heloisa M. Rutigliano; Kelsy A. Leppo; Kira P. Morgado
Source:
Reproduction, fertility, and development 2022 v.34 no.8 pp. 608-618
ISSN:
1031-3613
Subject:
polymerase chain reaction,   etc   ; endometrium; gene expression; interleukin-10; pregnancy;     Show all 5 Subjects
Abstract:
... This study aimed to determine the differences in gene expression between mononuclear cells derived from peripheral blood and endometrium during pregnancy in cattle and to determine the proportion of mononuclear cells in the endometrium of pregnant and diestrous cows. Endometrial tissue and peripheral blood were collected from Day 34±2 pregnant cows, and mononuclear cell populations were quant ...
DOI:
10.1071/RD21161

20. Development of a Duplex Digital PCR Method to Quantify Five Genetically Modified Soybean Events

Author:
Likun Long; Wei Yan; Yuxuan He; Liming Dong; Zhenjuan Xing; Congcong Li; Wei Xia; Feiwu Li
Source:
Food analytical methods 2022 v.15 no.2 pp. 294-306
ISSN:
1936-9751
Subject:
quantitative polymerase chain reaction,   etc   ; commercialization; droplets; fluorescence; genes; soybeans;     Show all 6 Subjects
Abstract:
... The presence of genetically modified organisms (GMOs) in food and feed products is regulated in many countries. Various countries have issued labeling threshold levels to supervise GMO commercialization, and efficient detection methods to quantify GMO contents are critical to meet labeling regulations. The aim of this study was to develop event-specific methods to measure the event-specific/lectin ...
DOI:
10.1007/s12161-021-02104-2