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You searched for: Subject "polypeptides" Remove constraint Subject: "polypeptides"
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1. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization

Author:
Dušanka A. Popović Minić; Danijel D. Milinčić; Stefan Kolašinac; Vladislav Rac; Jovana Petrović; Marina Soković; Nebojša Banjac; Jelena Lađarević; Bojana B. Vidović; Aleksandar Ž. Kostić; Vladimir B. Pavlović; Mirjana B. Pešić
Source:
Food chemistry 2023 v.402 pp. 134299
ISSN:
0308-8146
Subject:
polypeptides,   etc   ; Agaricus subrufescens; Fourier transform infrared spectroscopy; electron microscopy; electrophoresis; food chemistry; glycosides; goat milk; hydrogen; hydrophobicity; microstructure; milk; milk proteins; particle size; polysaccharides; proteolysis;     Show all 16 Subjects
Abstract:
... This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean par ...
DOI:
10.1016/j.foodchem.2022.134299

2. Reconstruction of mechanical unfolding and refolding pathways of proteins with atomic force spectroscopy and computer simulations

Author:
Qing Li; Dimitra Apostolidou; Piotr E. Marszalek
Source:
Methods 2022 v.197 pp. 39-53
ISSN:
1046-2023
Subject:
polypeptides,   etc   ; computers; proteome; spectroscopy;     Show all 4 Subjects
Abstract:
... Most proteins in proteomes are large, typically consist of more than one domain and are structurally complex. This often makes studying their mechanical unfolding pathways challenging. Proteins composed of tandem repeat domains are a subgroup of multi-domain proteins that, when stretched, display a saw-tooth pattern in their mechanical unfolding force extension profiles due to their repetitive str ...
DOI:
10.1016/j.ymeth.2021.05.012

3. Sequencing the origins of life

Author:
Tony Z. Jia; Shota Nishikawa; Kosuke Fujishima
Source:
BBA advances 2022 v.2 pp. 100049
ISSN:
2667-1603
Subject:
polypeptides,   etc   ; biopolymers; evolution; polymerization;     Show all 4 Subjects
Abstract:
... One goal of origins of life research is to understand how primitive informational and catalytic biopolymers emerged and evolved. Recently, a number of sequencing techniques have been applied to analysis of replicating and evolving primitive biopolymer systems, providing a sequence-specific and high-resolution view of primitive chemical processes. Here, we review application of sequencing technique ...
DOI:
10.1016/j.bbadva.2022.100049

4. Pyrene Excimer Formation (PEF) and Its Application to the Study of Polypeptide Dynamics

Author:
Remi Casier; Jean Duhamel
Source:
Langmuir 2022 v.38 no.12 pp. 3623-3629
ISSN:
1520-5827
Subject:
polypeptides,   etc   ; dyes; fluorescence; models; polymers;     Show all 5 Subjects
Abstract:
... This Perspective describes how the fluorescence blob model (FBM) has been developed and applied over the past 30 years to characterize the long-range backbone dynamics (LRBD) of polymers in solution. In these experiments, the polymers are randomly labeled with the dye pyrene, which forms an excimer upon the encounter between an excited and a ground-state pyrenyl label inside a finite subvolume of ...
DOI:
10.1021/acs.langmuir.2c00129

5. Translocation of polyubiquitinated protein substrates by the hexameric Cdc48 ATPase

Author:
Zhejian Ji; Hao Li; Daniele Peterle; Joao A. Paulo; Scott B. Ficarro; Thomas E. Wales; Jarrod A. Marto; Steven P. Gygi; John R. Engen; Tom A. Rapoport
Source:
Molecular cell 2022 v.82 no.3 pp. 570-584.e8
ISSN:
1097-2765
Subject:
polypeptides,   etc   ; adenosinetriphosphatase; proteasome endopeptidase complex; ubiquitin;     Show all 4 Subjects
Abstract:
... The hexameric Cdc48 ATPase (p97 or VCP in mammals) cooperates with its cofactor Ufd1/Npl4 to extract polyubiquitinated proteins from membranes or macromolecular complexes for degradation by the proteasome. Here, we clarify how the Cdc48 complex unfolds its substrates and translocates polypeptides with branchpoints. The Cdc48 complex recognizes primarily polyubiquitin chains rather than the attache ...
DOI:
10.1016/j.molcel.2021.11.033

6. End-Sealing of Peptide Nanotubes by Cationic Amphiphilic Polypeptides and Their Salt-Responsive Accordion-like Opening and Closing Behavior

Author:
Kon Son; Shinji Takeoka; Yoshihiro Ito; Motoki Ueda
Source:
Biomacromolecules 2022 v.23 no.7 pp. 2785-2792
ISSN:
1526-4602
Subject:
polypeptides,   etc   ; electrostatic interactions; hydrophilicity; nanocapsules; nanotubes; seals;     Show all 6 Subjects
Abstract:
... One strategy to prepare phase-separated co-assembly is to use the existing assembly as a platform to architect structures. For this purpose, the edge of a sheet or tube-shaped molecular assembly, which is less hydrophilic than the bulk region can become a starting point to build assembly units to realize more complex structures. In this study, we succeeded in preparing rod-shaped nanocapsules with ...
DOI:
10.1021/acs.biomac.2c00153

7. Dynamic Compartmentalization of Peptide–Oligonucleotide Conjugates with Reversible Nanovesicle–Microdroplet Phase Transition Behaviors

Author:
Bin Wang; Honglong Fang; Weiping Zhu; Yufang Xu; Yangyang Yang; Xuhong Qian
Source:
ACS applied materials & interfaces 2022 v.14 no.32 pp. 36998-37008
ISSN:
1944-8252
Subject:
polypeptides,   etc   ; oligonucleotides; phase transition; polysaccharides; separation; temperature;     Show all 6 Subjects
Abstract:
... Developing artificial microsystems based on liquid–liquid phase separation (LLPS) to mimic cellular dynamic compartmentalization has gained increasing attention. However, limitations including complicated components and laborious fabrication techniques have hindered their development. Herein, we describe a new single-component dynamic compartmentalization system using peptide–oligonucleotide conju ...
DOI:
10.1021/acsami.2c05268

8. Chirality of Superhelices Self-Assembled from Polypeptide Mixtures

Author:
Rui Hu; Chunhua Cai; Jiaping Lin; Liang Gao
Source:
Macromolecules 2022 v.55 no.11 pp. 4297-4304
ISSN:
1520-5835
Subject:
polypeptides,   etc   ; composite polymers; optical isomerism; solvents; temperature;     Show all 5 Subjects
Abstract:
... Superhelices with tunable chirality have been constructed; however, the formation mechanism of superhelical chirality is complicated, especially for assembly systems containing multiple chiral components. Revealing the contributions of each chiral component to superhelical chirality remains a challenge. Herein, we report that mixtures of polypeptide homopolymers and block copolymers can coassemble ...
DOI:
10.1021/acs.macromol.1c02639

9. Controlled biomolecules separation by CO2-responsive block copolymer membranes

Author:
Xiangyue Ye; Jiemei Zhou; Chenxu Zhang; Yong Wang
Source:
Journal of membrane science 2022 v.662 pp. 121022
ISSN:
0376-7388
Subject:
polypeptides,   etc   ; composite polymers; fractionation; nanopores; porosity; ultrafiltration;     Show all 6 Subjects
Abstract:
... The intelligent responsive membranes have aroused much attention due to their distinctive capability to reversibly change separation performances under the stimulation of ambient environment. Especially, the responsive membranes derived from block copolymers, which have regular nanoporous structures, display great potential in high-precision controllable separation. Herein, we use CO₂ gas as the n ...
DOI:
10.1016/j.memsci.2022.121022

10. Next generation single-molecule techniques: Imaging, labeling, and manipulation in vitro and in cellulo

Author:
Taekjip Ha; Christian Kaiser; Sua Myong; Bin Wu; Jie Xiao
Source:
Molecular cell 2022 v.82 no.2 pp. 304-314
ISSN:
1097-2765
Subject:
polypeptides,   etc   ; energy transfer; fluorescence; gene expression regulation; genomics;     Show all 5 Subjects
Abstract:
... Owing to their unique abilities to manipulate, label, and image individual molecules in vitro and in cellulo, single-molecule techniques provide previously unattainable access to elementary biological processes. In imaging, single-molecule fluorescence resonance energy transfer (smFRET) and protein-induced fluorescence enhancement in vitro can report on conformational changes and molecular interac ...
DOI:
10.1016/j.molcel.2021.12.019

11. Short-Range Backbone Dihedral Rotations Modulate Internal Friction in Intrinsically Disordered Proteins

Author:
Debapriya Das; Lisha Arora; Samrat Mukhopadhyay
Source:
Journal of the American Chemical Society 2022 v.144 no.4 pp. 1739-1747
ISSN:
1520-5126
Subject:
polypeptides,   etc   ; anisotropy; fluorescence; friction; models; proline; solvents; viscosity;     Show all 8 Subjects
Abstract:
... Protein folding and dynamics are governed by an intricate interplay of thermal and viscosity-mediated effects. The solvent viscosity contributes to the frictional drag in protein dynamics. In addition to this viscosity-dependent effect, there is also an intriguing viscosity-independent component that represents the intrinsic resistance of the polypeptide chain to changing its conformation. This so ...
DOI:
10.1021/jacs.1c11236

12. Small-molecule non-peptide antagonists of the PACAP receptor attenuate acute restraint stress-induced anxiety-like behaviors in mice

Author:
Yusuke Shintani; Yui Yamano; Manato kuta; Rei Takeshita; Kazuhiro Takuma; Takuya Okada; Naoki Toyooka; Ichiro Takasaki; Atsuro Miyata; Takashi Kurihara; Hitoshi Hashimoto; Atsuko Hayata-Takano
Source:
Biochemical and biophysical research communications 2022 v.631 pp. 146-151
ISSN:
0006-291X
Subject:
polypeptides,   etc   ; anxiety; fluoxetine; neuropeptides; psychological stress; research; tranquilizers;     Show all 7 Subjects
Abstract:
... Pituitary adenylate cyclase-activating polypeptide (PACAP) is a highly conserved pleiotropic neuropeptide, implicated in emotional stress responses and anxiety-related disorders. Here, we examined whether our recently developed small-molecule non-peptide PACAP receptor antagonists could ameliorate anxiety-like behaviors induced by acute restraint stress in mice. The antagonists PA-9 and its deriva ...
DOI:
10.1016/j.bbrc.2022.09.079

13. Systematic Identification of Microproteins during the Development of Drosophila melanogaster

Author:
Zhiwei Wang; Ni Pan; Jiahao Yan; Jian Wan; Cuihong Wan
Source:
Journal of proteome research 2022 v.21 no.4 pp. 1114-1123
ISSN:
1535-3907
Subject:
polypeptides,   etc   ; Drosophila melanogaster; oviposition; proteome; research; synthetic peptides;     Show all 6 Subjects
Abstract:
... Short open reading frame-encoded peptides (SEPs) are microproteins with less than 100 amino acids that play an essential role in the growth and development of organisms. There are plenty of short open reading frames in Drosophila melanogaster that potentially code polypeptides. We chose 11 time points during the life cycle of Drosophila to investigate microproteins, particularly those related to d ...
DOI:
10.1021/acs.jproteome.2c00004

14. A Tunable, Particle-Based Model for the Diverse Conformations Exhibited by Chiral Homopolymers

Author:
Natalie Buchanan; Joules Provenzano; Poornima Padmanabhan
Source:
Macromolecules 2022 v.55 no.15 pp. 6321-6331
ISSN:
1520-5835
Subject:
polypeptides,   etc   ; composite polymers; molecular dynamics; optical isomerism; thermodynamics;     Show all 5 Subjects
Abstract:
... Chiral block copolymers capable of hierarchical self-assembly can also exhibit chirality transfer─the transfer of chirality at the monomer or conformational scale to the self-assembly. Prior studies focused on experimental and theoretical methods that are unable to fully decouple the thermodynamic origins of chirality transfer and necessitate the development of particle-based models that can be us ...
DOI:
10.1021/acs.macromol.2c00613

15. Effects of Nanometer Confinement on the Self-Assembly and Dynamics of Poly(γ-benzyl-l-glutamate) and Its Copolymer with Poly(isobutylene)

Author:
Marianna Spyridakou; Kostas Tsimenidis; Manos Gkikas; Martin Steinhart; Robert Graf; George Floudas
Source:
Macromolecules 2022 v.55 no.7 pp. 2615-2626
ISSN:
1520-5835
Subject:
polypeptides,   etc   ; X-ray diffraction; composite polymers; dielectric spectroscopy;     Show all 4 Subjects
Abstract:
... Poly(γ-benzyl-l-glutamate) (PBLG) and its copolymer with poly(isobutylene) (PIB) are studied in the bulk and under nanometer confinement in pores with emphasis on the self-assembly and dynamics, respectively, with X-ray diffraction, ¹³C NMR, dielectric spectroscopy, and differential scanning calorimetry. PBLG segments located within the α-helical and amorphous regions have distinct dielectric fing ...
DOI:
10.1021/acs.macromol.2c00077

16. Biflavones inhibit the fibrillation and cytotoxicity of the human islet amyloid polypeptide

Author:
Jufei Xu; Yanan Wang; Ting Zheng; Yan Huo; Weihong Du
Source:
Journal of materials chemistry B 2022 v.10 no.24 pp. 4650-4661
ISSN:
2050-7518
Subject:
polypeptides,   etc   ; amyloid; amyloidosis; cytotoxicity; flavones; humans; hydrogen; hydrophobicity; oligomerization;     Show all 9 Subjects
Abstract:
... Biflavones are a kind of natural compound with a variety of biological activities, which have the capability of reversing diabetes and neurodegenerative diseases. The human islet amyloid polypeptide (hIAPP) is closely related to the pathological process of type II diabetes mellitus (T2DM). The development of new inhibitors is crucial to prevent hIAPP aggregation against T2DM. However, the influenc ...
DOI:
10.1039/d2tb00230b

17. Conformations of disulfides are conserved in inhibitory cystine knot (ICK) motif polypeptides

Author:
Shamasoddin Shekh; Smriti Moi; Panchada Ch V Govindu; Konkallu Hanumae Gowd
Source:
Toxicon 2022 v.219 pp. 106926
ISSN:
0041-0101
Subject:
polypeptides,   etc   ; X-radiation; cysteine; cystine; nuclear magnetic resonance spectroscopy;     Show all 5 Subjects
Abstract:
... The inhibitory cystine knot (ICK) motif is an evolutionarily optimized disulfide-rich peptide motif widely present in diverse phyla with distinct biological functions. Cysteine disulfides are highly conserved in the ICK motif with C₁–C₄ (Disulfide-I), C₂–C₅(Disulfide-II), and C₃–C₆(Disulfide-III) connectivities in a sequence. Disulfide-I and disulfide-II form a loop and the disulfide-III tethers t ...
DOI:
10.1016/j.toxicon.2022.09.012

18. Engineering multifunctional enzymes for agro-biomass utilization

Author:
Aarjoo Sharma; Sanjeev Balda; Neena Capalash; Prince Sharma
Source:
Bioresource technology 2022 v.347 pp. 126706
ISSN:
0960-8524
Subject:
polypeptides,   etc   ; biomass; cost effectiveness; lignocellulose; renewable resources; secretion; technology;     Show all 7 Subjects
Abstract:
... Lignocellulosic biomass is a plentiful renewable resource that can be converted into a wide range of high-value-added industrial products. However, the complexity of its structural integrity is one of the major constraints and requires combinations of different fibrolytic enzymes for the cost-effective, industrially and environmentally feasible transformation. An interesting approach is constructi ...
DOI:
10.1016/j.biortech.2022.126706

19. Membrane-Catalyzed Aggregation of Islet Amyloid Polypeptide Is Dominated by Secondary Nucleation

Author:
Barend O. W. Elenbaas; Lucie Khemtemourian; J. Antoinette Killian; Tessa Sinnige
Source:
Biochemistry 2022 v.61 no.14 pp. 1465-1472
ISSN:
1520-4995
Subject:
polypeptides,   etc   ; amyloid; death; kinetics; lipids; models; therapeutics; toxicity; zwitterions;     Show all 9 Subjects
Abstract:
... Type II diabetes is characterized by the loss of pancreatic β-cells. This loss is thought to be a consequence of membrane disruption, caused by the aggregation of islet amyloid polypeptide (IAPP) into amyloid fibrils. However, the molecular mechanisms of IAPP aggregation in the presence of membranes have remained unclear. Here, we use kinetic analysis to elucidate the aggregation mechanism of IAPP ...
DOI:
10.1021/acs.biochem.2c00184

20. Resource efficient collagen extraction from common starfish with the aid of high shear mechanical homogenization and ultrasound

Author:
Naveen Kumar Vate; Ingrid Undeland; Mehdi Abdollahi
Source:
Food chemistry 2022 v.393 pp. 133426
ISSN:
0308-8146
Subject:
polypeptides,   etc   ; Asteroidea; collagen; food chemistry; homogenization; thermal stability; ultrasonics;     Show all 7 Subjects
Abstract:
... Processes currently used for collagen extraction are complicated requiring a great deal of time and chemicals. Here, high shear mechanical homogenization (HSMH) and ultrasound (US) were integrated in the pretreatment step of collagen extraction from common starfish to reduce chemical use and time consumption. Effects of the assistant technologies on yield, structural integrity and functionality of ...
DOI:
10.1016/j.foodchem.2022.133426