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Dušanka A. Popović Minić; Danijel D. Milinčić; Stefan Kolašinac; Vladislav Rac; Jovana Petrović; Marina Soković; Nebojša Banjac; Jelena Lađarević; Bojana B. Vidović; Aleksandar Ž. Kostić; Vladimir B. Pavlović; Mirjana B. Pešić
... This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean par ...
... Most proteins in proteomes are large, typically consist of more than one domain and are structurally complex. This often makes studying their mechanical unfolding pathways challenging. Proteins composed of tandem repeat domains are a subgroup of multi-domain proteins that, when stretched, display a saw-tooth pattern in their mechanical unfolding force extension profiles due to their repetitive str ...
polypeptides, etc ; biopolymers; evolution; polymerization; Show all 4 Subjects
Abstract:
... One goal of origins of life research is to understand how primitive informational and catalytic biopolymers emerged and evolved. Recently, a number of sequencing techniques have been applied to analysis of replicating and evolving primitive biopolymer systems, providing a sequence-specific and high-resolution view of primitive chemical processes. Here, we review application of sequencing technique ...
polypeptides, etc ; dyes; fluorescence; models; polymers; Show all 5 Subjects
Abstract:
... This Perspective describes how the fluorescence blob model (FBM) has been developed and applied over the past 30 years to characterize the long-range backbone dynamics (LRBD) of polymers in solution. In these experiments, the polymers are randomly labeled with the dye pyrene, which forms an excimer upon the encounter between an excited and a ground-state pyrenyl label inside a finite subvolume of ...
polypeptides, etc ; adenosinetriphosphatase; proteasome endopeptidase complex; ubiquitin; Show all 4 Subjects
Abstract:
... The hexameric Cdc48 ATPase (p97 or VCP in mammals) cooperates with its cofactor Ufd1/Npl4 to extract polyubiquitinated proteins from membranes or macromolecular complexes for degradation by the proteasome. Here, we clarify how the Cdc48 complex unfolds its substrates and translocates polypeptides with branchpoints. The Cdc48 complex recognizes primarily polyubiquitin chains rather than the attache ...
polypeptides, etc ; electrostatic interactions; hydrophilicity; nanocapsules; nanotubes; seals; Show all 6 Subjects
Abstract:
... One strategy to prepare phase-separated co-assembly is to use the existing assembly as a platform to architect structures. For this purpose, the edge of a sheet or tube-shaped molecular assembly, which is less hydrophilic than the bulk region can become a starting point to build assembly units to realize more complex structures. In this study, we succeeded in preparing rod-shaped nanocapsules with ...
polypeptides, etc ; oligonucleotides; phase transition; polysaccharides; separation; temperature; Show all 6 Subjects
Abstract:
... Developing artificial microsystems based on liquid–liquid phase separation (LLPS) to mimic cellular dynamic compartmentalization has gained increasing attention. However, limitations including complicated components and laborious fabrication techniques have hindered their development. Herein, we describe a new single-component dynamic compartmentalization system using peptide–oligonucleotide conju ...
polypeptides, etc ; composite polymers; optical isomerism; solvents; temperature; Show all 5 Subjects
Abstract:
... Superhelices with tunable chirality have been constructed; however, the formation mechanism of superhelical chirality is complicated, especially for assembly systems containing multiple chiral components. Revealing the contributions of each chiral component to superhelical chirality remains a challenge. Herein, we report that mixtures of polypeptide homopolymers and block copolymers can coassemble ...
polypeptides, etc ; composite polymers; fractionation; nanopores; porosity; ultrafiltration; Show all 6 Subjects
Abstract:
... The intelligent responsive membranes have aroused much attention due to their distinctive capability to reversibly change separation performances under the stimulation of ambient environment. Especially, the responsive membranes derived from block copolymers, which have regular nanoporous structures, display great potential in high-precision controllable separation. Herein, we use CO₂ gas as the n ...
polypeptides, etc ; energy transfer; fluorescence; gene expression regulation; genomics; Show all 5 Subjects
Abstract:
... Owing to their unique abilities to manipulate, label, and image individual molecules in vitro and in cellulo, single-molecule techniques provide previously unattainable access to elementary biological processes. In imaging, single-molecule fluorescence resonance energy transfer (smFRET) and protein-induced fluorescence enhancement in vitro can report on conformational changes and molecular interac ...
polypeptides, etc ; anisotropy; fluorescence; friction; models; proline; solvents; viscosity; Show all 8 Subjects
Abstract:
... Protein folding and dynamics are governed by an intricate interplay of thermal and viscosity-mediated effects. The solvent viscosity contributes to the frictional drag in protein dynamics. In addition to this viscosity-dependent effect, there is also an intriguing viscosity-independent component that represents the intrinsic resistance of the polypeptide chain to changing its conformation. This so ...
polypeptides, etc ; anxiety; fluoxetine; neuropeptides; psychological stress; research; tranquilizers; Show all 7 Subjects
Abstract:
... Pituitary adenylate cyclase-activating polypeptide (PACAP) is a highly conserved pleiotropic neuropeptide, implicated in emotional stress responses and anxiety-related disorders. Here, we examined whether our recently developed small-molecule non-peptide PACAP receptor antagonists could ameliorate anxiety-like behaviors induced by acute restraint stress in mice. The antagonists PA-9 and its deriva ...
polypeptides, etc ; Drosophila melanogaster; oviposition; proteome; research; synthetic peptides; Show all 6 Subjects
Abstract:
... Short open reading frame-encoded peptides (SEPs) are microproteins with less than 100 amino acids that play an essential role in the growth and development of organisms. There are plenty of short open reading frames in Drosophila melanogaster that potentially code polypeptides. We chose 11 time points during the life cycle of Drosophila to investigate microproteins, particularly those related to d ...
polypeptides, etc ; composite polymers; molecular dynamics; optical isomerism; thermodynamics; Show all 5 Subjects
Abstract:
... Chiral block copolymers capable of hierarchical self-assembly can also exhibit chirality transfer─the transfer of chirality at the monomer or conformational scale to the self-assembly. Prior studies focused on experimental and theoretical methods that are unable to fully decouple the thermodynamic origins of chirality transfer and necessitate the development of particle-based models that can be us ...
polypeptides, etc ; X-ray diffraction; composite polymers; dielectric spectroscopy; Show all 4 Subjects
Abstract:
... Poly(γ-benzyl-l-glutamate) (PBLG) and its copolymer with poly(isobutylene) (PIB) are studied in the bulk and under nanometer confinement in pores with emphasis on the self-assembly and dynamics, respectively, with X-ray diffraction, ¹³C NMR, dielectric spectroscopy, and differential scanning calorimetry. PBLG segments located within the α-helical and amorphous regions have distinct dielectric fing ...
polypeptides, etc ; amyloid; amyloidosis; cytotoxicity; flavones; humans; hydrogen; hydrophobicity; oligomerization; Show all 9 Subjects
Abstract:
... Biflavones are a kind of natural compound with a variety of biological activities, which have the capability of reversing diabetes and neurodegenerative diseases. The human islet amyloid polypeptide (hIAPP) is closely related to the pathological process of type II diabetes mellitus (T2DM). The development of new inhibitors is crucial to prevent hIAPP aggregation against T2DM. However, the influenc ...
polypeptides, etc ; X-radiation; cysteine; cystine; nuclear magnetic resonance spectroscopy; Show all 5 Subjects
Abstract:
... The inhibitory cystine knot (ICK) motif is an evolutionarily optimized disulfide-rich peptide motif widely present in diverse phyla with distinct biological functions. Cysteine disulfides are highly conserved in the ICK motif with C₁–C₄ (Disulfide-I), C₂–C₅(Disulfide-II), and C₃–C₆(Disulfide-III) connectivities in a sequence. Disulfide-I and disulfide-II form a loop and the disulfide-III tethers t ...
polypeptides, etc ; biomass; cost effectiveness; lignocellulose; renewable resources; secretion; technology; Show all 7 Subjects
Abstract:
... Lignocellulosic biomass is a plentiful renewable resource that can be converted into a wide range of high-value-added industrial products. However, the complexity of its structural integrity is one of the major constraints and requires combinations of different fibrolytic enzymes for the cost-effective, industrially and environmentally feasible transformation. An interesting approach is constructi ...
polypeptides, etc ; amyloid; death; kinetics; lipids; models; therapeutics; toxicity; zwitterions; Show all 9 Subjects
Abstract:
... Type II diabetes is characterized by the loss of pancreatic β-cells. This loss is thought to be a consequence of membrane disruption, caused by the aggregation of islet amyloid polypeptide (IAPP) into amyloid fibrils. However, the molecular mechanisms of IAPP aggregation in the presence of membranes have remained unclear. Here, we use kinetic analysis to elucidate the aggregation mechanism of IAPP ...
polypeptides, etc ; Asteroidea; collagen; food chemistry; homogenization; thermal stability; ultrasonics; Show all 7 Subjects
Abstract:
... Processes currently used for collagen extraction are complicated requiring a great deal of time and chemicals. Here, high shear mechanical homogenization (HSMH) and ultrasound (US) were integrated in the pretreatment step of collagen extraction from common starfish to reduce chemical use and time consumption. Effects of the assistant technologies on yield, structural integrity and functionality of ...