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- Author:
- Vaselek, Slavica; Prudhomme, Jorian; Myskova, Jitka; Lestinova, Tereza; Spitzova, Tatiana; Bañuls, Anne-Laure; Volf, Petr; Florin, David
- Source:
- Journal of medical entomology 2020 v.57 no.2 pp. 601-607
- ISSN:
- 1938-2928
- Subject:
- promastigotes, etc ; Leishmania infantum; Phlebotomus perniciosus; amastigotes; biosafety; cages; comparative study; containers; females; hematophagy; parasites; Show all 11 Subjects
- Abstract:
- ... Sand flies (Diptera: Psychodidae) are natural vectors of Leishmania. For the initiation of sand fly experimental infections either Leishmania amastigotes or promastigotes can be used. In order to obtain comparable results, it is necessary to adjust and standardize procedures. During this study, we conducted promastigote- and amastigote-initiated infections of Leishmania infantum Nicolle, 1908 para ...
- DOI:
- 10.1093/jme/tjz199
- PubMed:
- 31702779
- PubMed Central:
- PMC7044723
- https://dx.doi.org/10.1093/jme/tjz199
- Author:
- Paape, Daniel; Prendergast, Catriona T.; Price, Helen P.; Doehl, Johannes S. P.; Smith, Deborah F.
- Source:
- Parasites & vectors 2020 v.13 no.1 pp. 132
- ISSN:
- 1756-3305
- Subject:
- promastigotes, etc ; Leishmania; RNA interference; drug development; genes; genomics; loci; macrophages; mice; models; mutants; parasites; plasmids; selection pressure; transferases; viability; Show all 16 Subjects
- Abstract:
- ... BACKGROUND: Proving that specific genes are essential for the intracellular viability of Leishmania parasites within macrophages remains a challenge for the identification of suitable targets for drug development. This is especially evident in the absence of a robust inducible expression system or functioning RNAi machinery that works in all Leishmania species. Currently, if a target gene of inter ...
- DOI:
- 10.1186/s13071-020-3999-1
- PubMed:
- 32171322
- PubMed Central:
- PMC7071782
- https://dx.doi.org/10.1186/s13071-020-3999-1
- Author:
- Hendrickx, Sarah; Van Bockstal, Lieselotte; Bulté, Dimitri; Mondelaers, Annelies; Aslan, Hamide; Rivas, Luis; Maes, Louis; Caljon, Guy
- Source:
- Parasites & vectors 2020 v.13 no.1 pp. 96
- ISSN:
- 1756-3305
- Subject:
- promastigotes, etc ; Leishmania donovani; Lutzomyia longipalpis; chromosomes; drugs; genes; macrophages; parasite load; parasites; phenotype; relapse; therapeutics; virulence; visceral leishmaniasis; India; Show all 15 Subjects
- Abstract:
- ... BACKGROUND: Since the introduction of miltefosine (MIL) as first-line therapy in the kala-azar elimination programme in the Indian subcontinent, treatment failure rates have been increasing. Since parasite infectivity and virulence may become altered upon treatment relapse, this laboratory study assessed the phenotypic effects of repeated in vitro and in vivo MIL exposure. METHODS: Syngeneic Leish ...
- DOI:
- 10.1186/s13071-020-3972-z
- PubMed:
- 32087758
- PubMed Central:
- PMC7036194
- https://dx.doi.org/10.1186/s13071-020-3972-z
- Author:
- Becvar, Tomas; Siriyasatien, Padet; Bates, Paul; Volf, Petr; Sádlová, Jovana
- Source:
- Parasites & vectors 2020 v.13 no.1 pp. 181
- ISSN:
- 1756-3305
- Subject:
- promastigotes, etc ; DNA; Leishmania enriettii; Lutzomyia; animal diseases; disease reservoirs; ears; erythema; guinea pigs; humans; leishmaniasis; models; parasites; polymerase chain reaction; tissues; wild animals; Ghana; Show all 17 Subjects
- Abstract:
- ... BACKGROUND: Leishmaniasis is a human and animal disease caused by parasites of the genus Leishmania, which is now divided into four subgenera, Leishmania, Viannia, Sauroleishmania and Mundinia. Subgenus Mundinia, established in 2016, is geographically widely dispersed, its distribution covers all continents, except Antarctica. It consists of 5 species; L. enriettii and L. macropodum are parasites ...
- DOI:
- 10.1186/s13071-020-04039-9
- PubMed:
- 32268916
- PubMed Central:
- PMC7140393
- https://dx.doi.org/10.1186/s13071-020-04039-9
- Author:
- Bott, Emanuel; López, María Gabriela; Lammel, Estela María; Carfagna, Ivanna Emilce; Durante de Isola, Elvira Luisa; Ruybal, Paula; Taboga, Oscar; Gimenez, Guadalupe; Belaunzarán, María Laura
- Source:
- Microbial pathogenesis 2020 v.141 pp. 104010
- ISSN:
- 0882-4010
- Subject:
- promastigotes, etc ; Baculoviridae; Leishmania braziliensis; Trypanosoma; amastigotes; catalytic activity; genes; leishmaniasis; nucleotide sequences; parasites; phosphatidylcholines; phospholipase A1; phospholipases; phylogeny; recombinant proteins; Latin America; Show all 16 Subjects
- Abstract:
- ... Leishmaniasis is caused by several species of protozoan parasites of the genus Leishmania and represents an important global health problem. Leishmania braziliensis in particular is responsible of cutaneous and mucocutaneous forms of this parasitosis, with prevalence in Latin America. In the present work, we describe in L. braziliensis promastigotes and amastigotes the presence of a Phospholipase ...
- DOI:
- 10.1016/j.micpath.2020.104010
- https://dx.doi.org/10.1016/j.micpath.2020.104010
6. Drug-containing hydrophobic dressings as a topical experimental therapy for cutaneous leishmaniasis
- Author:
- Pereira, Viviane; de Barros, Neuza Biguinati; Macedo, Sharon Rose Aragão; dos Santos Ferreira, Amália; Kanis, Luiz Alberto; Nicolete, Roberto
- Source:
- Journal of parasitic diseases 2020 v.44 no.1 pp. 79-87
- ISSN:
- 0971-7196
- Subject:
- promastigotes, etc ; Leishmania amazonensis; adverse effects; amastigotes; blood serum; cutaneous leishmaniasis; drugs; histology; hydrophobicity; intravenous injection; macrophages; mice; parasite load; patients; topical application; Brazil; Show all 16 Subjects
- Abstract:
- ... Cutaneous leishmaniasis (CL), a clinical condition caused mainly by Leishmania amazonensis in Brazil, is characterized by topical, painless ulcers. The current treatment, based on intravenous administration of pentavalent antimonials, presents low adherence by patients and may cause serious adverse effects, leading to the need for searching new therapeutic options. Thus, this study aimed at evalua ...
- DOI:
- 10.1007/s12639-019-01162-y
- PubMed:
- 32174708
- PubMed Central:
- PMC7046828
- http://dx.doi.org/10.1007/s12639-019-01162-y
- Author:
- Hashiguchi, Yoshihisa; Hashiguchi, Kazue; Zambrano, Flavio C.; Parraga, Francisco D.; Martillo, Viriginia P.; Torres, Edison X.; Velez, Lenin N.; Villegas, Nancy V.; Gomez, Eduardo A.; Kato, Hirotomo
- Source:
- Acta tropica 2020 v.203 pp. 105321
- ISSN:
- 0001-706X
- Subject:
- promastigotes, etc ; Leishmania mexicana; Lutzomyia ayacuchensis; blood meal; cutaneous leishmaniasis; cytochrome b; dry season; genes; hindgut; microscopy; midgut; parasites; planning; temperature; vector control; wet season; Andes region; Ecuador; Show all 18 Subjects
- Abstract:
- ... To elucidate the transmission mode of Andean cutaneous leishmaniasis (Andean-CL), natural Leishmania infection and biting activity of sand flies were tested in a selected sylvatic focus of the endemic area of the Ecuadorian Andes. Monthly sand fly collections and dissections were conducted during 12 months from July 2018 to June 2019. The Leishmania positive specimens/slides with innumerable amoun ...
- DOI:
- 10.1016/j.actatropica.2019.105321
- https://dx.doi.org/10.1016/j.actatropica.2019.105321
- Author:
- Smirlis, Despina; Dingli, Florent; Pescher, Pascale; Prina, Eric; Loew, Damarys; Rachidi, Najma; Späth, Gerald F.
- Source:
- Journal of proteomics 2020 v.213 pp. 103617
- ISSN:
- 1874-3919
- Subject:
- promastigotes, etc ; Leishmania donovani; anion exchange; drug therapy; fractionation; gel electrophoresis; humans; leishmaniasis; macrophages; metabolism; mice; morbidity; mortality; parasites; pathogens; phenotype; proteins; proteome; proteomics; tropical diseases; vacuoles; Show all 21 Subjects
- Abstract:
- ... Leishmaniases are major vector-borne tropical diseases responsible for great human morbidity and mortality, caused by protozoan, trypanosomatid parasites of the genus Leishmania. In the mammalian host, parasites survive and multiply within mononuclear phagocytes, especially macrophages. However, the underlying mechanisms by which Leishmania spp. affect their host are not fully understood. Herein, ...
- DOI:
- 10.1016/j.jprot.2019.103617
- https://dx.doi.org/10.1016/j.jprot.2019.103617
- Author:
- Pessanha de Carvalho, Laís; Held, Jana; de Melo, Edésio José Tenório
- Source:
- Experimental parasitology 2020 v.209 pp. 107826
- ISSN:
- 0014-4894
- Subject:
- promastigotes, etc ; Leishmania amazonensis; animal diseases; cadmium chloride; heterochromatin; mercuric chloride; metabolism; metal ions; microbodies; mitochondria; new drugs; parasites; parasitism; toxicity; vertebrates; zinc; zinc chloride; Show all 17 Subjects
- Abstract:
- ... Protozoan parasites like Leishmania amazonensis are excellent models to test the effects of new drugs against a functional molecular arsenal used to establish successfully an infection in the vertebrate host, where they invade the cells of the monocytic system. However, little is known about the influence of metal ions on the cellular functionality of the infective forms of L. amazonensis. In the ...
- DOI:
- 10.1016/j.exppara.2019.107826
- https://dx.doi.org/10.1016/j.exppara.2019.107826
- Author:
- Wei, Furong; Gong, Wenci; Wang, Junyun; Yang, Yuetao; Liu, Jianxiu; Wang, Yanjuan; Cao, Jianping
- Source:
- Parasites & vectors 2019 v.12 no.1 pp. 275
- ISSN:
- 1756-3305
- Subject:
- promastigotes, etc ; Leishmania infantum; antagonists; extrusion; histones; inflammation; leishmaniasis; mechanism of action; neutrophils; therapeutics; Show all 10 Subjects
- Abstract:
- ... BACKGROUND: Neutrophils play an immunomodulatory role through the release of neutrophil extracellular traps (NETs). NETs are released in response to Leishmania infection, but the mechanism of NET extrusion has not been elucidated. The lipoxin A4 receptor on neutrophils is crucial for the inflammatory response and immune regulation of many diseases, including Leishmania infection. Therefore, in the ...
- DOI:
- 10.1186/s13071-019-3530-8
- PubMed:
- 31142352
- PubMed Central:
- PMC6542009
- https://dx.doi.org/10.1186/s13071-019-3530-8
- Author:
- Aoki, Juliana Ide; Laranjeira-Silva, Maria Fernanda; Muxel, Sandra Marcia; Floeter-Winter, Lucile Maria
- Source:
- Current opinion in microbiology 2019 v.52 pp. 110-115
- ISSN:
- 1369-5274
- Subject:
- promastigotes, etc ; Leishmania amazonensis; amastigotes; arginase; enzyme activity; immune response; parasites; polyamines; transcriptomics; vaccines; virulence; Show all 11 Subjects
- Abstract:
- ... The outcome of Leishmania infection depends on the parasite species and the host immune response. Virulence factors have been extensively studied over the years in an effort to find efficient vaccines and/or treatments for Leishmania infection. Arginase activity in Leishmania has been described as an essential player for the polyamines pathway, impacting parasite replication and infectivity. Consi ...
- DOI:
- 10.1016/j.mib.2019.06.003
- https://dx.doi.org/10.1016/j.mib.2019.06.003
- Author:
- Zafar, Salman; -Ur-Rehman, Faiz; Shah, Zafar Ali; Rauf, Abdur; Khan, Aziz; Humayun Khan, Muhammad; Ur Rahman, Khaliq; Khan, Serab; Ullah, Abid; Shaheen, Farzana
- Source:
- Journal of Asian natural products research 2019 v.21 no.7 pp. 679-687
- ISSN:
- 1477-2213
- Subject:
- promastigotes, etc ; Leishmania tropica; Olea; antibacterial properties; chromatography; ethyl acetate; metabolites; pathogens; spectroscopy; Show all 9 Subjects
- Abstract:
- ... Inspired from the leishmanicidal and antibacterial potential of the fractions obtained from the crude extract of Olea ferruginea stem, the anti-leishmanial ethyl acetate fraction was subjected to chromatographic separation, leading to the isolation of a new compound ferruginan (1) and a known compound (+)- cycloolivil (2). The structures of 1 and 2 were determined by various spectroscopic techniqu ...
- DOI:
- 10.1080/10286020.2018.1467894
- http://dx.doi.org/10.1080/10286020.2018.1467894
- Author:
- Sereno, Denis; Harrat, Zoubir; Eddaikra, Naouel
- Source:
- Acta tropica 2019 v.191 pp. 204-211
- ISSN:
- 0001-706X
- Subject:
- promastigotes, etc ; Leishmania; amastigotes; antibiotic resistance; genetic markers; guidelines; meta-analysis; microorganisms; phenotype; Show all 9 Subjects
- Abstract:
- ... Antimicrobial resistance (AMR) threatens the prevention and treatment of infections caused by a large range of microorganisms. Leishmania is not an exception and treatment failure due to drug-resistant organisms is increasingly reported. Currently, no molecular methods and marker are validated to track drug-resistant organism and antimicrobial susceptibility tests are roughly not amenable to a cli ...
- DOI:
- 10.1016/j.actatropica.2019.01.009
- https://dx.doi.org/10.1016/j.actatropica.2019.01.009
- Author:
- Olivier, Martin; Menendez, Aida Minguez; Fernandez-Prada, Christopher
- Source:
- Trends in parasitology 2019
- ISSN:
- 1471-4922
- Subject:
- promastigotes, etc ; Leishmania; Leishmania RNA virus; Lutzomyia; amastigotes; exosomes; females; hematophagy; leishmaniasis; parasites; tissues; Argentina; United States; Show all 13 Subjects
- Abstract:
- ... Leishmania of the Viannia subgenus, including Leishmania Viannia guyanensis, is the agent responsible for cutaneous and mucocutaneous leishmaniasis (CL and MCL) in the Americas from the US to Argentina. 48,000 new cases of Cl and MCL are reported yearly, among which 1/10 are associated with L. V. guyanensis infection transmitted by female Lutzomyia sandflies during the blood meal. Inoculated metac ...
- DOI:
- 10.1016/j.pt.2019.06.008
- http://dx.doi.org/10.1016/j.pt.2019.06.008
- Author:
- Zheng, Zhi-Wan; Li, Jiao; Chen, Han; He, Jin-Lei; Chen, Qi-Wei; Zhang, Jian-Hui; Zhou, Qi; Chen, Da-Li; Chen, Jian-Ping
- Source:
- Parasites & vectors 2020 v.13 no.1 pp. 94
- ISSN:
- 1756-3305
- Subject:
- promastigotes, etc ; Leishmania donovani; adverse effects; amastigotes; antileishmanials; cell lines; cyclophilin A; cyclosporine; cytotoxicity; hydrogen peroxide; inhibitory concentration 50; interferon-gamma; interleukin-10; interleukin-12; interleukin-4; macrophages; mice; mitochondria; nitric oxide; sodium; therapeutics; tumor necrosis factor-alpha; ultrastructure; visceral leishmaniasis; Show all 24 Subjects
- Abstract:
- ... BACKGROUND: New therapeutic drugs are urgently needed against visceral leishmaniasis because current drugs, such as pentavalent antimonials and miltefosine, produce severe side effects and development of resistance. Whether cyclosporine A (CsA) and its derivatives can be used as therapeutic drugs for visceral leishmaniasis has been controversial for many years. METHODS: In this study, we evaluated ...
- DOI:
- 10.1186/s13071-020-3958-x
- PubMed:
- 32085719
- PubMed Central:
- PMC7035640
- https://dx.doi.org/10.1186/s13071-020-3958-x
- Author:
- Alves-Ferreira, Eliza Vanessa Carneiro; Ferreira, Tiago Rodrigues; Walrad, Pegine; Kaye, Paul M.; Cruz, Angela Kaysel
- Source:
- Parasites & vectors 2020 v.13 no.1 pp. 9
- ISSN:
- 1756-3305
- Subject:
- promastigotes, etc ; Leishmania braziliensis; Leishmania donovani; Western blotting; amastigotes; arachidonic acid; computer simulation; cytoplasm; enzymes; gene expression; heat stress; homeostasis; host-parasite relationships; humans; image analysis; inflammation; lipid metabolism; macrophages; metabolites; mice; microscopy; parasites; pathogenesis; pathogenicity; prostaglandins; proteome; Show all 26 Subjects
- Abstract:
- ... BACKGROUND: Prostaglandins (PG) are lipid mediators derived from arachidonic acid metabolism. They are involved in cellular processes such as inflammation and tissue homeostasis. PG production is not restricted to multicellular organisms. Trypanosomatids also synthesize several metabolites of arachidonic acid. Nevertheless, their biological role in these early-branching parasites and their role in ...
- DOI:
- 10.1186/s13071-020-3883-z
- PubMed:
- 31915065
- PubMed Central:
- PMC6950890
- https://dx.doi.org/10.1186/s13071-020-3883-z
- Author:
- Nkwenti Wonkam, Argan Kelly; Njanpa Ngansop, Cyrille Armel; Njonte Wouamba, Steven Collins; Jouda, Jean Bosco; Happi, Gervais Mouthe; Boyom, Fabrice Fekam; Sewald, Norbert; Lenta, Bruno Ndjakou
- Source:
- Biochemical systematics and ecology 2020 v.93 pp. 104137
- ISSN:
- 0305-1978
- Subject:
- promastigotes, etc ; Leishmania donovani; Rubiaceae; antileishmanial properties; beta-sitosterol; ceramides; cytotoxicity; erythrodiol; in vitro studies; inhibitory concentration 50; kaempferol; leaves; lupeol; macrophages; mannitol; methanol; methylene chloride; nuclear magnetic resonance spectroscopy; palmitates; stigmasterol; ursolic acid; Show all 21 Subjects
- Abstract:
- ... The chemical investigation of the CH₂Cl₂/MeOH (1:1) extract of the leaves of Rothmannia hispida (K. Schum.) Fagerl. (Rubiaceae) led to the isolation of a new ceramide rothmanniamide (1) and a naturally isolated alkyl cinnamate derivative n-heptadecyl-4-hydroxy-trans-cinnamate (2), along with fifteen known compounds including lupeol palmitate (3), lupeol (4), a mixture of uvaol (5) and erythrodiol ...
- DOI:
- 10.1016/j.bse.2020.104137
- https://dx.doi.org/10.1016/j.bse.2020.104137
- Author:
- Sanchiz, África; Morato, Esperanza; Rastrojo, Alberto; Camacho, Esther; González-de la Fuente, Sandra; Marina, Anabel; Aguado, Begoña; Requena, Jose M.
- Source:
- Genes 2020 v.11 no.9
- ISSN:
- 2073-4425
- Subject:
- promastigotes, etc ; Leishmania infantum; RNA-binding proteins; amino acid sequences; databases; enzymes; flagellum; genes; genomics; mass spectrometry; microbodies; mitochondria; molecular chaperones; nucleotide sequences; peptides; phosphorylation; post-translational modification; proteome; proteomics; transcriptomics; translation (genetics); visceral leishmaniasis; Show all 22 Subjects
- Abstract:
- ... Leishmania infantum causes visceral leishmaniasis (kala-azar), the most severe form of leishmaniasis, which is lethal if untreated. A few years ago, the re-sequencing and de novo assembling of the L. infantum (JPCM5 strain) genome was accomplished, and now we aimed to describe and characterize the experimental proteome of this species. In this work, we performed a proteomic analysis from axenic cu ...
- DOI:
- 10.3390/genes11091036
- http://dx.doi.org/10.3390/genes11091036
- Author:
- Ahmed, Humera; Carter, Katharine C.; Williams, Roderick A.M.
- Source:
- Microorganisms 2020 v.8 no.8
- ISSN:
- 2076-2607
- Subject:
- promastigotes, etc ; Leishmania donovani; adverse effects; amastigotes; antileishmanial properties; antimony; bone marrow; carbon; chemical structure; cytotoxicity; diarrhea; inhibitory concentration 50; intravenous injection; liver; mice; models; oral administration; parasite load; parasites; spleen; teratogenicity; visceral leishmaniasis; vomiting; Show all 23 Subjects
- Abstract:
- ... Miltefosine (Milt) is the only oral treatment for visceral leishmaniasis (VL) but its use is associated with adverse effects, e.g., teratogenicity, vomiting, diarrhoea. Understanding how its chemical structure induces cytotoxicity, whilst not compromising its anti-parasitic efficacy, could identify more effective compounds. Therefore, we systemically modified the compound’s head, tail and linker t ...
- DOI:
- 10.3390/microorganisms8081117
- http://dx.doi.org/10.3390/microorganisms8081117
- Author:
- KarimiPourSaryazdi, Amir; Ghaffarifar, Fatemeh; Dalimi, Abdolhossein; Dayer, Mohammad Saaid
- Source:
- Journal of ethnopharmacology 2020 v.257 pp. 112910
- ISSN:
- 0378-8741
- Subject:
- promastigotes, etc ; Artemisia aucheri; Leishmania major; amastigotes; antioxidants; apoptosis; autumn; coumarin; cytotoxicity; death; flow cytometry; high performance liquid chromatography; in vitro studies; inhibitory concentration 50; macrophages; mice; ointments; plant extracts; quercitrin; spring; survival rate; therapeutics; traditional medicine; Show all 23 Subjects
- Abstract:
- ... Artemisia aucheri Bioss contains flavonoid, coumarin and santonin with antioxidant, antimicrobial and antileishmanial effects. The current study was aimed to comparatively evaluate the effects of spring and autumn extracts of A. aucheri Bioss on Leishmania major both in-vitro and in-vivo conditions.HPLC analysis was used to evaluate the percentages of compounds in spring and autumn extracts of A. ...
- DOI:
- 10.1016/j.jep.2020.112910
- https://dx.doi.org/10.1016/j.jep.2020.112910