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1. The Trinity of SPRTN Protease Regulation

Author:
Ruggiano, Annamaria; Ramadan, Kristijan
Source:
Trends in biochemical sciences 2021 v.46 no.1 pp. 2-4
ISSN:
0968-0004
Subject:
proteolysis,   etc   ; crosslinking; proteinases;     Show all 3 Subjects
Abstract:
... The protease SPRTN emerged as the essential enzyme for DNA–protein crosslink proteolysis repair. Biochemical and cell biological work indicated that SPRTN is a nonspecific protease. Recent and independent studies from Lou, Stingele, and Ramadan reveal that SPRTN activity is modulated via three layers of regulation that make it selective for DNA–protein crosslinks. ...
DOI:
10.1016/j.tibs.2020.10.007

2. The metalloproteinase ADAM10 requires its activity to sustain surface expression

Author:
Seifert, Anke; Düsterhöft, Stefan; Wozniak, Justyna; Koo, Chek Z.; Tomlinson, Michael G.; Nuti, Elisa; Rossello, Armando; Cuffaro, Doretta; Yildiz, Daniela; Ludwig, Andreas
Source:
Cellular and molecular life sciences 2021 v.78 no.2 pp. 715-732
ISSN:
1420-682X
Subject:
proteolysis,   etc   ; inflammation; metalloproteinases; temperature; therapeutics;     Show all 5 Subjects
Abstract:
... The metalloproteinase ADAM10 critically contributes to development, inflammation, and cancer and can be controlled by endogenous or synthetic inhibitors. Here, we demonstrate for the first time that loss of proteolytic activity of ADAM10 by either inhibition or loss of function mutations induces removal of the protease from the cell surface and the whole cell. This process is temperature dependent ...
DOI:
10.1007/s00018-020-03507-w

3. Potential application of non-thermal atmospheric plasma in reducing the activity of Pseudomonas-secreted proteases in milk

Author:
Mohammadpour, Hooriyeh; Zarei, Mehdi; Cullen, Patrick J.; Valtchev, Peter; Schindeler, Aaron; Dehghani, Fariba
Source:
International dairy journal 2021 v.120 pp. 105078
ISSN:
0958-6946
Subject:
proteolysis,   etc   ; UHT milk; hydrolysis; milk; proteinases; whey;     Show all 6 Subjects
Abstract:
... Application of non-thermal atmospheric plasma was examined against the activity of Pseudomonas-secreted proteases in milk. UHT milk samples were inoculated with crude extract of Pseudomonas-secreted proteases and treated in atmospheric plasma discharge system. Evaluation of the progress in protein hydrolysis of plasma-treated and non-treated milk samples revealed that the degree of hydrolysis was ...
DOI:
10.1016/j.idairyj.2021.105078

4. In Vitro Selection of Macrocyclic d/l-Hybrid Peptides against Human EGFR

Author:
Imanishi, Sayaka; Katoh, Takayuki; Yin, Yizhen; Yamada, Mituhiro; Kawai, Marina; Suga, Hiroaki
Source:
Journal of the American Chemical Society 2021 v.143 no.15 pp. 5680-5684
ISSN:
1520-5126
Subject:
proteolysis,   etc   ; humans; peptides; protein-protein interactions;     Show all 4 Subjects
Abstract:
... d/l-Hybrid peptides are an attractive class of molecular modality because they are able to exhibit high proteolytic stability and unique structural diversity which cannot be accessed by those consisting of only proteinogenic l-amino acids. Despite such an expectation, it has not been possible to devise de novo d/l-hybrid peptides capable of disrupting the function of a protein target(s) due to the ...
DOI:
10.1021/jacs.1c02593

5. Molecular mechanisms regulating Proteinase‐Activated Receptors (PARs)

Author:
Chandrabalan, Arundhasa; Ramachandran, Rithwik
Source:
TheFEBS journal 2021 v.288 no.8 pp. 2697-2726
ISSN:
1742-464X
Subject:
proteolysis,   etc   ; drug development; structure-activity relationships;     Show all 3 Subjects
Abstract:
... Proteinase‐activated receptors (PARs) are a four‐member family of G protein‐coupled receptors defined by their irreversible proteolytic mechanism of activation. PARs have emerged as important regulators of various physiological responses and are implicated in numerous pathological conditions. Importantly, PAR1 and PAR4 are critical regulators of platelet function, while PAR2 is well established as ...
DOI:
10.1111/febs.15829

6. RssB-mediated σS Activation is Regulated by a Two-Tier Mechanism via Phosphorylation and Adaptor Protein – IraD

Author:
Wang, Zhihao; Zhao, Siyu; Li, Yanqing; Zhang, Kaining; Mo, Fei; Zhang, Jiye; Hou, Yajing; He, Langchong; Liu, Zhijun; Wang, Yawen; Xu, Yingqi; Wang, Hongliang; Buck, Martin; Matthews, Steve J.; Liu, Bing
Source:
Journal of molecular biology 2021 v.433 no.3 pp. 166757
ISSN:
0022-2836
Subject:
proteolysis,   etc   ; models; molecular biology; mutation; phosphorylation;     Show all 5 Subjects
Abstract:
... Regulation of bacterial stress responding σS is a sophisticated process and mediated by multiple interacting partners. Controlled proteolysis of σS is regulated by RssB which maintains minimal level of σS during exponential growth but then elevates σS level while facing stresses. Bacteria developed different strategies to regulate activity of RssB, including phosphorylation of itself and productio ...
DOI:
10.1016/j.jmb.2020.166757

7. Defense and Offense Strategies: The Role of Aspartic Proteases in Plant–Pathogen Interactions

Author:
Figueiredo, Laura; Santos, Rita B.; Figueiredo, Andreia
Source:
Biology 2021 v.10 no.2
ISSN:
2079-7737
Subject:
proteolysis,   etc   ; abiotic stress; active sites; aspartic proteinases;     Show all 4 Subjects
Abstract:
... Plant aspartic proteases (APs; E.C.3.4.23) are a group of proteolytic enzymes widely distributed among different species characterized by the conserved sequence Asp-Gly-Thr at the active site. With a broad spectrum of biological roles, plant APs are suggested to undergo functional specialization and to be crucial in developmental processes, such as in both biotic and abiotic stress responses. Over ...
DOI:
10.3390/biology10020075

8. Expression of calpastatin isoforms in three skeletal muscles of Angus steers and their association with fiber type composition and proteolytic potential

Author:
Motter, Mariana M.; Corva, Pablo M.; Soria, Liliana A.
Source:
Meat science 2021 v.171 pp. 108267
ISSN:
0309-1740
Subject:
proteolysis,   etc   ; Angus; calpastatin; exons; meat science; muscles;     Show all 6 Subjects
Abstract:
... This study aimed to evaluate the expression of calpastatin (CAST) isoforms and their potential associations with fiber type composition (%RA), calpastatin activity (CA) and myofibril fragmentation index (MFI) in three muscles with known differences in tenderness (infraspinatus, triceps brachii and semitendinosus) of Angus steers. Expression of total CAST (CAST-T) and CAST isoforms I, II, III (2–3) ...
DOI:
10.1016/j.meatsci.2020.108267

9. μ-Calpain oxidation and proteolytic changes on myofibrillar proteins from Coregonus Peled in vitro

Author:
Liu, Pingping; Zhang, Zhiwei; Guo, Xin; Zhu, Xinrong; Mao, Xiaoying; Guo, Xiaobing; Deng, Xiaorong; Zhang, Jian
Source:
Food chemistry 2021 v.361 pp. 130100
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; Coregonus; absorption; fluorescence; food chemistry; hydroxyl radicals; oxidation;     Show all 7 Subjects
Abstract:
... The purpose of this study was to investigate the structural properties of μ-calpain induced by hydroxyl radical oxidation and its effect on the degradation of myofibrillar protein (MP) from the dorsal muscles of Coregonus peled. The carbonyl and sulfhydryl content of μ-calpain changed significantly after oxidation. The content of α-helix in the secondary structure decreased from 0.825 to 0.232 and ...
DOI:
10.1016/j.foodchem.2021.130100

10. Effect of Yarrowia lipolytica RO25 cricket-based hydrolysates on sourdough quality parameters

Author:
Rossi, Samantha; Parrotta, Luigi; Del Duca, Stefano; Rosa, Marco Dalla; Patrignani, Francesca; Schluter, Oliver; Lanciotti, Rosalba
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.148 pp. 111760
ISSN:
0023-6438
Subject:
proteolysis,   etc   ; Orthoptera; Yarrowia lipolytica; breads; hydrolysates; odors; protein content; sourdough;     Show all 8 Subjects
Abstract:
... The principal aim of this research was to use Yarrowia lipolytica RO25 to obtain a cricket powder-based hydrolysate to produce sourdough for bread production. RO25 hydrolysed cricket sourdough (RO25H-CS) was compared with a control traditional sourdough and a control containing the no-hydrolysed cricket powder. Microbiological analyses evidenced a good growth of Yarrowia lipolytica in RO25 cricket ...
DOI:
10.1016/j.lwt.2021.111760

11. From Green Revolution to Green Balance: The Nitrogen and Gibberellin Mediated Rice Tiller Growth

Author:
Huang, Li-Jun; Luo, Jianjun; Wang, Yukun; Li, Ning
Source:
Plant signaling & behavior 2021 v.16 no.7 pp. 1917838
ISSN:
1559-2324
Subject:
proteolysis,   etc   ; behavior; dwarfing; nitrogen; proteasome endopeptidase complex; rice; tillering;     Show all 7 Subjects
Abstract:
... Rice tillering is an important characteristic that responds to both GA (gibberellin) and nitrogen-based fertilizers. How plants balance these two responses? A newly identified NGR5 (NITROGEN-MEDIATED TILLER GROWTH RESPONSE 5) protein reveals its important role in controlling the balance between GA-regulated dwarfism and nitrogen-regulated tillering. NGR5 directly interacts with PRC2 (Polycomb Repr ...
DOI:
10.1080/15592324.2021.1917838

12. Analysis of the proteolytic system of Streptococcus thermophilus strains CS5, CS9, CS18 and CS20

Author:
Hu, Tong; Cui, Yanhua; Qu, Xiaojun
Source:
International dairy journal 2021 v.118 pp. 105025
ISSN:
0958-6946
Subject:
proteolysis,   etc   ; Streptococcus thermophilus; fermentation; gene expression; genes; milk; transcription factors;     Show all 7 Subjects
Abstract:
... Streptococcus thermophilus is a common milk starter, and the ability to hydrolyse proteins is an important property. The proteolytic systems of S. thermophilus strains CS5, CS9, CS18 and CS20 have been investigated according to their genomes. The growth and proteolytic abilities of these strains have been analysed in combination with gene expressions of both the proteolytic system and the two-comp ...
DOI:
10.1016/j.idairyj.2021.105025

13. The p105 NF-ĸB precursor is a pseudo substrate of the ubiquitin ligase FBXO7, and its binding to the ligase stabilizes it and results in stimulated cell proliferation

Author:
Udasin, Ronald G.; Gottfried, Yossi; Fabre, Bertrand; Bercovich, Beatrice; Ziv, Tamar; Ciechanover, Aaron
Source:
Biochemical and biophysical research communications 2021 v.558 pp. 224-230
ISSN:
0006-291X
Subject:
proteolysis,   etc   ; cell proliferation; inflammation; mutants; research; ubiquitin-protein ligase;     Show all 6 Subjects
Abstract:
... The NF-κB transcription factor is involved in inflammation and cell proliferation, survival, and transformation. It is a heterodimer made of p50 or p52 and a member of the Rel family of proteins. p50 and p52 are derived from limited ubiquitin- and proteasome-mediated proteolytic processing of the larger precursors p105 and p100, respectively. Both precursors can be either processed or completely d ...
DOI:
10.1016/j.bbrc.2020.08.098

14. Effects of shredding on the functionality, microstructure and proteolysis of low-moisture mozzarella cheese

Author:
Pax, Anita P.; Ong, Lydia; Kentish, Sandra E.; Gras, Sally L.
Source:
International dairy journal 2021 v.117 pp. 104979
ISSN:
0958-6946
Subject:
proteolysis,   etc   ; carbon dioxide; cellulose; microstructure; mozzarella cheese; proteinases; vacuum packaging;     Show all 7 Subjects
Abstract:
... Low-moisture mozzarella cheese (LMMC) is commonly shredded before packaging, however, the effects of shredding are not fully understood. Industrially-produced block and shredded LMMC were studied during 8 weeks of storage at 4 °C. Cheese shredded on 15 d and at 8 weeks of age, coated with microcrystalline cellulose and stored in a modified atmosphere (70% N₂ and 30% CO₂), had an altered microstruc ...
DOI:
10.1016/j.idairyj.2021.104979

15. Amyloids: The History of Toxicity and Functionality

Author:
Yakupova, Elmira I.; Bobyleva, Liya G.; Shumeyko, Sergey A.; Vikhlyantsev, Ivan M.; Bobylev, Alexander G.
Source:
Biology 2021 v.10 no.5
ISSN:
2079-7737
Subject:
proteolysis,   etc   ; amyloid; amyloidosis; chemical structure; polypeptides; proteinases; therapeutics; toxicity;     Show all 8 Subjects
Abstract:
... Proteins can perform their specific function due to their molecular structure. Partial or complete unfolding of the polypeptide chain may lead to the misfolding and aggregation of proteins in turn, resulting in the formation of different structures such as amyloid aggregates. Amyloids are rigid protein aggregates with the cross-β structure, resistant to most solvents and proteases. Because of thei ...
DOI:
10.3390/biology10050394

16. Determining MMP‐2 and MMP‐9 reductive activities of bovine and equine amniotic membranes homogenates using fluorescence resonance energy transfer

Author:
Capistrano da Silva, Erotides; Gibson, Daniel J.; Jeong, Sunyoung; Zimmerman, Kelli L.; Smith‐Fleming, Kathryn M.; Martins, Bianca da C.
Source:
Veterinary ophthalmology 2021 v.24 no.3 pp. 279-287
ISSN:
1463-5216
Subject:
proteolysis,   etc   ; ; cattle; cornea; energy transfer; fluorescence; horses; metalloproteinases; ophthalmology;     Show all 9 Subjects
Abstract:
... INTRODUCTION: Matrix metalloproteinases (MMPs)‐2 and ‐9 are present in corneal ulcers, and an imbalance between MMPs and tissue inhibitors of metalloproteinases (TIMPs) leads to further corneal degradation. Amniotic membrane homogenate (AMH) has proteolytic properties beneficial for corneal healing, but it is unknown whether AMH possesses TIMPs or effectively inhibits MMP‐2 and MMP‐9 activity. OBJ ...
DOI:
10.1111/vop.12888

17. Flow Cytometric Assessment of the Viability and Functionality of Uterine Polymorphonuclear Leukocytes in Postpartum Dairy Cows

Author:
Lietaer, Leen; Demeyere, Kristel; Heirbaut, Stijn; Meyer, Evelyne; Opsomer, Geert; Bogado Pascottini, Osvaldo
Source:
Animals 2021 v.11 no.4
ISSN:
2076-2615
Subject:
proteolysis,   etc   ; Holstein; apoptosis; endometrium; flow cytometry; fluorescence; milk; phagocytosis; viability;     Show all 9 Subjects
Abstract:
... Postpartum dairy cows experience impaired peripheral polymorphonuclear leukocyte (PMN) functionality, which has been associated with reproductive tract inflammatory diseases. However, it has not been elucidated yet whether endometrial PMN functionality is (equally) impaired. We developed a method for endometrial PMN isolation and flow cytometric assessment of their viability and functionality. We ...
DOI:
10.3390/ani11041081

18. Reduction in the Brining Time in Parmigiano Reggiano Cheese Production Minimally Affects Proteolysis, with No Effect on Sensory Properties

Author:
Loffi, Cecilia; Bortolazzo, Elena; Garavaldi, Anna; Musi, Valeria; Reverberi, Paolo; Galaverna, Gianni; Sforza, Stefano; Tedeschi, Tullia
Source:
Foods 2021 v.10 no.4
ISSN:
2304-8158
Subject:
proteolysis,   etc   ; Parmesan cheese; nutrient content; peptides; salt content;     Show all 5 Subjects
Abstract:
... Brine soaking is one of the most important steps in the production of Parmigiano Reggiano cheese, since it determines the amount of salt in the final product. Reduction in salt in Parmigiano Reggiano cheese might be important for improving its nutritional profile, but it could affect the manufacturing processes by altering proteolysis and consequently the product quality. In this study, for the fi ...
DOI:
10.3390/foods10040770

19. A novel proteolytic cleavage of ROCK 1 in cell death: Not only by caspases 3 and 7 but also by caspase 2

Author:
Özdemir, Aysun; İbişoğlu, Burçin; Şimay Demir, Yaprak Dilber; Benhür, Elifnur; Valipour, Farzaneh; Ark, Mustafa
Source:
Biochemical and biophysical research communications 2021 v.547 pp. 118-124
ISSN:
0006-291X
Subject:
proteolysis,   etc   ; apoptosis; caspase-3; myosin light chains; phosphorylation; research;     Show all 6 Subjects
Abstract:
... During apoptosis, myosin light chain phosphorylation induced by ROCK 1, activated by caspase 3-mediated cleavage, results in the formation of membrane blebs. Additionally, actin-myosin-based contraction induced by the activation of ROCK is involved in the apoptotic nuclear disintegration. In previous studies, it was reported that ROCK 1 was only cleaved by caspase 3 in cell death and caspase 7 was ...
DOI:
10.1016/j.bbrc.2021.02.024

20. Tune instead of destroy: How proteolysis keeps OXPHOS in shape

Author:
Szczepanowska, Karolina; Trifunovic, Aleksandra
Source:
Biochimica et biophysica acta 2021 v.1862 no.4 pp. 148365
ISSN:
0005-2728
Subject:
proteolysis,   etc   ; dynamics; mitochondria; mitophagy; proteinases; shape; stress response;     Show all 7 Subjects
Abstract:
... Mitochondria are highly dynamic and stress-responsive organelles that are renewed, maintained and removed by a number of different mechanisms. Recent findings bring more evidence for the focused, defined, and regulatory function of the intramitochondrial proteases extending far beyond the traditional concepts of damage control and stress responses. Until recently, the macrodegradation processes, s ...
DOI:
10.1016/j.bbabio.2020.148365