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1. Lys-C/Arg-C, a More Specific and Efficient Digestion Approach for Proteomics Studies

Author:
Wu, Zhen; Huang, Jichang; Huang, Jingnan; Li, Qingqing; Zhang, Xumin
Source:
Analytical chemistry 2018 v.90 no.16 pp. 9700-9707
ISSN:
1520-6882
Subject:
proteolysis,   etc   ; proteomics; trypsin;     Show all 3 Subjects
Abstract:
... Nowadays, bottom-up approaches are predominantly adopted in proteomics studies, which necessitate a proteolysis step prior to MS analysis. Trypsin is often the best protease in choice due to its high specificity and MS-favored proteolytic products. A lot of efforts have been made to develop a superior digestion approach but hardly succeed, especially in large-scale proteomics studies. Herein, we r ...
DOI:
10.1021/acs.analchem.8b02448

2. A novel and sensitive functional assay for complement Factor I based on the third proteolytic clip of C3b

Author:
Lachmann, Peter J.; Lay, Elizabeth; Seilly, David J.
Source:
Journal of immunological methods 2018
ISSN:
0022-1759
Subject:
proteolysis,   etc   ; complement; ionic strength;     Show all 3 Subjects
Abstract:
... A sensitive assay for the functional activity of complement Factor I is described. This is based on its third proteolytic clip whereby Factor I cleaves cell-bound iC3b to cell-bound C3dg and soluble C3c, thereby abolishing conglutination of the cells. Factor H is required as a co-factor for Factor I activity. Because of the low affinity of iC3b for Factor H, the assay needs to be performed at low ...
DOI:
10.1016/j.jim.2018.03.013

3. Effect of nitric oxide and calpastatin on the inhibition of µ-calpain activity, autolysis and proteolysis of myofibrillar proteins

Author:
Liu, Rui; Lonergan, Steven; Steadham, Edward; Zhou, Guanghong; Zhang, Wangang; Huff-Lonergan, Elisabeth
Source:
Food chemistry 2019 v.275 pp. 77-84
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; autolysis; calcium; calpastatin; dose response; myofibrillar proteins; nitric oxide;     Show all 7 Subjects
Abstract:
... The aim of this study was to investigate the dual effect of the nitric oxide donor NOR-3 and calpastatin on µ-calpain activity, autolysis, and proteolytic ability. µ-Calpain and calpastatin were purified and allocated to the following five treatments: µ-calpain, µ-calpain + calpastatin, µ-calpain + NOR-3, µ-calpain + calpastatin + NOR-3, and µ-calpain + NOR-3 + calpastatin. µ-Calpain autolysis and ...
DOI:
10.1016/j.foodchem.2018.09.104

4. DNA recovery from gelatin fingerprint lifters by direct proteolytic digestion

Author:
Zieger, Martin; Schneider, Christoph; Utz, Silvia
Source:
Forensic science international 2019 v.295 pp. 145-149
ISSN:
0379-0738
Subject:
proteolysis,   etc   ; DNA; DNA fingerprinting; forensic sciences; gelatin; magnetism; protocols; staining;     Show all 8 Subjects
Abstract:
... Fingerprints are a valuable source for DNA profiling in forensic investigations. In practice, the fingerprints are routinely visualized first by powder staining and then often transferred to tapes or gelatin lifters for storage or examination. If at all, fingerprints are usually sampled for DNA in a second step. To target the DNA sampling in an optimal way, it is essential to know how much of the ...
DOI:
10.1016/j.forsciint.2018.12.006

5. Development of a method for the quantification of fish major allergen parvalbumin in food matrix via liquid chromatography-tandem mass spectrometry with multiple reaction monitoring

Author:
Sun, Lirui; Lin, Hong; Li, Zhenxing; Sun, Weihong; Wang, Jianhua; Wu, Haiyan; Ge, Minmin; Ahmed, Ishfaq; Pavase, Tushar Ramesh
Source:
Food chemistry 2019 v.276 pp. 358-365
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; Paralichthys olivaceus; allergens; flounder; food matrix; heat; liquid chromatography; monitoring; tandem mass spectrometry; trypsin;     Show all 10 Subjects
Abstract:
... The availability of analytical methods for quantification of allergens is crucial for the correct assessment and labeling of products in order to protect allergic consumers. For this purpose, a simple, sensitive and accurate technique was developed based on liquid chromatography-tandem mass spectrometry and multiple reaction monitoring mass spectrometry (LC-MRM-MS/MS). The proposed method uses a s ...
DOI:
10.1016/j.foodchem.2018.10.014

6. Effect of nitric oxide on myofibrillar proteins and the susceptibility to calpain-1 proteolysis

Author:
Liu, Rui; Lonergan, Steven; Steadham, Edward; Zhou, Guanghong; Zhang, Wangang; Huff-Lonergan, Elisabeth
Source:
Food chemistry 2019 v.276 pp. 63-70
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; crosslinking; desmin; muscles; myofibrillar proteins; nitric oxide; swine; thiols; troponin T;     Show all 9 Subjects
Abstract:
... This study was designed to investigate the nature of modification of myofibrillar proteins by nitric oxide (NO) and the extent to which S-nitrosylation alters their susceptibility to calpain-1 proteolysis. Isolated myofibrils from porcine semimembranosus muscle were incubated with the NO donor S-nitrosoglutathione (GSNO) at 0, 20, 50, 250, 1000 µM for 30 min at 37 °C and then incubated with purifi ...
DOI:
10.1016/j.foodchem.2018.10.005

7. A one-pot analysis approach to simplify measurements of protein stability and folding kinetics

Author:
Liu, Yi-Kai; Chen, Huei-Yu; Chueh, Pin Ju; Liu, Pei-Fen
Source:
Biochimica et biophysica acta 2019 v.1867 no.3 pp. 184-193
ISSN:
1570-9639
Subject:
proteolysis,   etc   ; dihydrofolate reductase; maltose; methotrexate; neoplasm cells; neoplasms; protein folding; proteomics; screening;     Show all 9 Subjects
Abstract:
... To achieve a good understanding of the characteristics of a protein, it is important to study its stability and folding kinetics. Investigations of protein stability have been recently applied to drug-target identification, drug screening, and proteomic studies. The efficiency of the experiments performed to study protein stability and folding kinetics is now a crucial factor that needs to be opti ...
DOI:
10.1016/j.bbapap.2018.12.006

8. Calpastatin inhibits the activity of phosphorylated μ-calpain in vitro

Author:
Du, Manting; Li, Xin; Li, Zheng; Shen, Qingwu; Ren, Chi; Zhang, Dequan
Source:
Food chemistry 2019 v.274 pp. 743-749
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; alkaline phosphatase; cAMP-dependent protein kinase; calpastatin; heat stability; phosphorylation; proteins;     Show all 7 Subjects
Abstract:
... The objective of this study was to investigate the effect of phosphorylation on the sensitivity of μ-calpain to the inhibition induced by calpastatin. Purified μ-calpain was incubated with alkaline phosphatase (AP) or protein kinase A (PKA) to modulate the phosphorylation level of μ-calpain. Accurately 25, 50, 100 and 150 units of AP/PKA-treated μ-calpain were mixed with the same amounts of heat s ...
DOI:
10.1016/j.foodchem.2018.09.073

9. Bispecific anti-mPDGFRβ x cotinine scFv-Cκ-scFv fusion protein and cotinine-duocarmycin can form antibody-drug conjugate-like complexes that exert cytotoxicity against mPDGFRβ expressing cells

Author:
Kim, Soohyun; Kim, Hyori; Jo, Dong Hyun; Kim, Jeong Hun; Kim, Su Ree; Kang, Dongmin; Hwang, Dobeen; Chung, Junho
Source:
Methods 2019 v.154 pp. 125-135
ISSN:
1046-2023
Subject:
proteolysis,   etc   ; antibodies; cotinine; cytotoxicity; drugs; platelet-derived growth factor receptor beta;     Show all 6 Subjects
Abstract:
... Antibody selection for antibody-drug conjugates (ADCs) has traditionally depended on its internalization into the target cell, although ADC efficacy also relies on recycling of the receptor-ADC complex, endo-lysosomal trafficking, and subsequent linker/antibody proteolysis. In this study, we observed that a bispecific anti-murine platelet-derived growth factor receptor beta (mPDGFRβ) x cotinine si ...
DOI:
10.1016/j.ymeth.2018.10.002

10. Proteolytic susceptibility of food by-product proteins: An evaluation by means of a quantitative index

Author:
Valencia, Pedro L.; Solis, Tamara; Rojas, Paula; Ibañez, Francisco; Astudillo-Castro, Carolina; Pinto, Marlene; Almonacid, Sergio
Source:
Process biochemistry 2019 v.77 pp. 63-69
ISSN:
1359-5113
Subject:
proteolysis,   etc   ; byproducts; chemical structure; equations; keratin; muscles; pH; protein sources; salmon; subtilisin; whey; whey protein;     Show all 12 Subjects
Abstract:
... A proteolytic susceptibility index was proposed to evaluate the reaction performance of different food by-products with subtilisin. Whey, salmon muscle and feather keratine were hydrolyzed at the same peptide bond concentration at 50 °C and pH 8.0 with different subtilisin concentrations. The logarithmic equation P = 1/b ln(abt+1) was fitted to estimate the kinetic constants a and b. The a/b ratio ...
DOI:
10.1016/j.procbio.2018.11.009

11. Towards understanding the effect of high pressure on food protein allergenicity: β-lactoglobulin structural studies

Author:
Kurpiewska, Katarzyna; Biela, Artur; Loch, Joanna I.; Lipowska, Joanna; Siuda, Monika; Lewiński, Krzysztof
Source:
Food chemistry 2019 v.270 pp. 315-321
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; allergenicity; allergens; beta-lactoglobulin; crystallography; dietary protein; epitopes; foods; myristic acid; physicochemical properties;     Show all 10 Subjects
Abstract:
... A number of studies were devoted to understanding an immunological effect of pressure-treated β-lactoglobulin. In our previous work we have proved that high pressure significantly modifies β-lactoglobulin conformation and consequently its physicochemical properties. Here, structure of β-lactoglobulin complex with myristic acid determined at the highest accepted by the crystal pressure value of 550 ...
DOI:
10.1016/j.foodchem.2018.07.104

12. Age Gelation, Sedimentation, and Creaming in UHT Milk: A Review

Author:
Anema, Skelte G.
Source:
Comprehensive reviews in food science and food safety 2019 v.18 no.1 pp. 140-166
ISSN:
1541-4337
Subject:
proteolysis,   etc   ; UHT milk; beverages; enzymes; gelation; gels; heat; heat stability; milk; milk composition; proteins; shelf life;     Show all 12 Subjects
Abstract:
... Demand for ultra‐high‐temperature (UHT) milk and milk protein‐based beverages is growing. UHT milk is microbiologically stable. However, on storage, a number of chemical and physical changes occur and these can reduce the quality of the milk. These changes can be sufficiently undesirable so as to limit acceptance or shelf life of the milk. The most severe changes in UHT milk during storage are age ...
DOI:
10.1111/1541-4337.12407

13. Comparative study of the insoluble and soluble Ulp1 protease constructs as Carrier free and dependent protein immobilizates

Author:
Jiang, Li; Xiao, Wenjun; Zhou, Xuan; Wang, Weiyu; Fan, Jun
Source:
Journal of bioscience and bioengineering 2019 v.127 no.1 pp. 23-29
ISSN:
1389-1723
Subject:
proteolysis,   etc   ; Escherichia coli; binding capacity; cellulose; crosslinking; glutaraldehyde; inclusion bodies; storage temperature; trypsin; yeasts;     Show all 10 Subjects
Abstract:
... In this study, we analyzed and compared the properties of yeast Ulp1 protease in active inclusion bodies (IBs) as special protein immobilizate, and the soluble Ulp1 via oriented immobilization. Fusion of the N-terminal self-assembling peptide GFIL8 to the Ulp1 increased production of active IBs in Escherichia coli. Attachment of the N-terminal cellulose-binding module facilitated the constructed p ...
DOI:
10.1016/j.jbiosc.2018.06.016

14. Porcine CD83 is a glycosylated dimeric protein existing naturally in membrane-bound and soluble forms

Author:
Huo, Shanshan; Zhang, Jianlou; Wu, Fengyang; Zuo, Yuzhu; Cui, Dan; Li, Xiujin; Zhong, Zhenyu; Zhong, Fei
Source:
Developmental and comparative immunology 2019 v.90 pp. 60-69
ISSN:
0145-305X
Subject:
proteolysis,   etc   ; T-lymphocytes; cysteine; disulfide bonds; eukaryotic cells; genes; glycosylation; humans; mice; swine;     Show all 10 Subjects
Abstract:
... Human and mouse CD83 have been well characteized, however, the other mammalian CD83 genes have not been cloned and characterized. In this study, the porcine CD83 (pCD83) was cloned, expressed and characterized, and showed that the pCD83 gene has 81% and 74% homologies with humans and mice, respectively, which was identified to be glycosylated when expressed in eukaryotic cells, existing naturally ...
DOI:
10.1016/j.dci.2018.09.002

15. The X-prolyl dipeptidyl-peptidase PepX of Streptococcus thermophilus initially described as intracellular is also responsible for peptidase extracellular activity

Author:
Hafeez, Zeeshan; Cakir-Kiefer, Céline; Lecomte, Xavier; Miclo, Laurent; Dary-Mourot, Annie
Source:
Journal of dairy science 2019 v.102 no.1 pp. 113-123
ISSN:
0022-0302
Subject:
proteolysis,   etc   ; Streptococcus thermophilus; enzyme activity; enzymes; fermentation; filtrates; genes; hydrolysis; lactose; milk; mutants; protein transport; proteins;     Show all 13 Subjects
Abstract:
... This study addresses the hypothesis that the extracellular cell-associated X-prolyl dipeptidyl-peptidase activity initially described in Streptococcus thermophilus could be attributable to the intracellular X-prolyl dipeptidyl-peptidase PepX. For this purpose, a PepX-negative mutant of S. thermophilus LMD-9 was constructed by interrupting the pepX gene and named LMD-9-ΔpepX. When cultivated, the S ...
DOI:
10.3168/jds.2018-14823

16. Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin

Author:
Hegemann, Julian D.; Schwalen, Christopher J.; Mitchell, Douglas A.; van der Donk, Wilfred A.
Source:
Chemical communications 2018 v.54 no.65 pp. 9007-9010
ISSN:
1364-548X
Subject:
proteolysis,   etc   ; biosynthesis; chemical compounds; macrocyclization reactions;     Show all 4 Subjects
Abstract:
... Substrate binding assays, in vitro proteolytic processing assays, and heterologous lasso peptide production were used to investigate the roles of conserved precursor peptide residues during paeninodin maturation. Specifically, we delineate which residues are important for substrate recognition, proteolysis, and lasso peptide macrocyclization. ...
DOI:
10.1039/c8cc04411b

17. A Dimethyl-Labeling-Based Strategy for Site-Specifically Quantitative Chemical Proteomics

Author:
Yang, Fan; Gao, Jinjun; Che, Jinteng; Jia, Guogeng; Wang, Chu
Source:
Analytical chemistry 2018 v.90 no.15 pp. 9576-9582
ISSN:
1520-6882
Subject:
proteolysis,   etc   ; isotope labeling; protein composition; proteome; proteomics;     Show all 5 Subjects
Abstract:
... Activity-based protein profiling (ABPP) has emerged as a powerful functional chemoproteomic strategy which enables global profiling of proteome reactivity toward bioactive small molecules in complex biological and/or pathological processes. To quantify the degree of reactivity in a site-specific manner, an isotopic tandem orthogonal proteolysis (isoTOP)-ABPP strategy has been developed; however, t ...
DOI:
10.1021/acs.analchem.8b02426

18. Reversible Lysine Derivatization Enabling Improved Arg-C Digestion, a Highly Specific Arg-C Digestion Using Trypsin

Author:
Wu, Zhen; Huang, Jichang; Lu, Jianan; Zhang, Xumin
Source:
Analytical chemistry 2018 v.90 no.3 pp. 1554-1559
ISSN:
1520-6882
Subject:
proteolysis,   etc   ; arginine; derivatization; lysine; proteome; proteomics; trypsin;     Show all 7 Subjects
Abstract:
... The bottom-up proteomics approach has become an important strategy in diverse areas of biological research, and the enzymatic digestion is essential for this technology. Endopeptidase Arg-C catalyzing the hydrolytic cleavage of peptide bonds C-terminal to arginine could be an important protease in bottom-up proteomics. However, it has been seldom applied due to its low specificity and high cost. I ...
DOI:
10.1021/acs.analchem.7b04410

19. Epsin-Dependent Ligand Endocytosis Activates Notch by Force

Author:
Langridge, Paul D.; Struhl, Gary
Source:
Cell 2017 v.171 no.6 pp. 1383-1396.e12
ISSN:
0092-8674
Subject:
proteolysis,   etc   ; endocytosis; ligands; models;     Show all 4 Subjects
Abstract:
... DSL ligands activate Notch by inducing proteolytic cleavage of the receptor ectodomain, an event that requires ligand to be endocytosed in signal-sending cells by the adaptor protein Epsin. Two classes of explanation for this unusual requirement are (1) recycling models, in which the ligand must be endocytosed to be modified or repositioned before it binds Notch and (2) pulling models, in which th ...
DOI:
10.1016/j.cell.2017.10.048

20. Endogenous peptide profile for elucidating biosynthetic processing of the ghrelin precursor

Author:
Tsuchiya, Takashi; Iwakura, Hiroshi; Minamino, Naoto; Kangawa, Kenji; Sasaki, Kazuki
Source:
Biochemical and biophysical research communications 2017
ISSN:
0006-291X
Subject:
proteolysis,   etc   ; exocytosis; ghrelin; mice;     Show all 4 Subjects
Abstract:
... Ghrelin is an orexigenic peptide primarily produced by gastric endocrine cells. The biosynthetic cleavage site of ghrelin has been well documented, but how its downstream region undergoes proteolytic processing remains poorly explored. Here, we provide the first snapshot of endogenous peptides from the ghrelin precursor by profiling the secretopeptidome of cultured mouse ghrelin-producing cells du ...
DOI:
10.1016/j.bbrc.2017.06.155