An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
proteolysis, etc ; digestion; elderly; enzymatic hydrolysis; food chemistry; hydrolysis; models; skim milk; stomach; young adults; Show all 10 Subjects
Abstract:
... This study aimed to evaluate gastric emptying and enzymatic hydrolysis of skim milk using a dynamic in vitro gastrointestinal system mimicking digestive conditions in young adult and elderly individuals. The gastric emptying half-time was 22.5 and 26.5 min in the young adult and elderly models, respectively. The degree of proteolysis at 120 min reached 42.3 % under adult digestion, significantly h ...
proteolysis, etc ; coproducts; food chemistry; gelation; gels; head; herring; lipid peroxidation; myosin; protein isolates; spring; Show all 11 Subjects
Abstract:
... Effect of catching season (spring vs fall), pre-sorting and selective recombination of different herring filleting co-products on protein yield during valorisation using the alkaline pH-shift technology was studied. Impacts of the pre-processing conditions on lipid oxidation, rheological, structural and functional properties of the proteins were also investigated. The sorted frame fraction resulte ...
proteolysis, etc ; acidification; beta-galactosidase; cold storage; galactose; glucose; lactose; lactose free diet; odors; taste; Show all 10 Subjects
Abstract:
... Many lactose-free dairy products are produced using commercial lactase, but not without problems such as the sweetish taste due to the presence of free glucose and galactose. The aim of the present study was to obtain a lactose-free version of direct-acidified mozzarella without using the enzyme, but removing lactose by curd washing and pressing. The best results were obtained with double curd was ...
... The effects of phenolic fraction of mulberry pomace on yogurt quality were investigated. Polyphenol-enriched extract of mulberry pomace (MPPE), rich in phenols, anthocyanins and proanthocyanidins, was incorporated to yogurt before and after fermentation at 0.50%, 0.75%, 1.00% and 1.25% (w/v), respectively. The addition either before or after fermentation decreased L* and b* values, increased a* va ...
... Yeasts play a critical role in the flavor formation of dry-cured ham. In this study, 41 yeast isolates from the dry-cured ham at different processing stages were evaluated for their technological properties. Debaryomyces hansenii was the most dominant yeast and has been detected at each phase of dry-cured ham, followed by Candida zeylanoides which was mainly detected in salting phase. Yarrowia bub ...
proteolysis, etc ; absorption; chemical bonding; circular dichroism spectroscopy; ferulic acid; heat treatment; hydrocolloids; ligands; lysine; molecular dynamics; pH; proteomics; temperature; Show all 13 Subjects
Abstract:
... The molecular nature of interactions between ferulic acid and β-lactoglobulin was investigated following exposure of solutions to high temperature (121 °C) at near neutral pH (7.3) using biochemical spectroscopy, proteomics and molecular dynamics. Circular dichroism (CD) and infrared spectroscopy (FTIR) argue for alterations in the secondary structure of the heated protein molecule in the presence ...
proteolysis, etc ; bioavailability; crosslinking; disulfide bonds; encapsulation; enzymatic hydrolysis; food chemistry; gastrointestinal system; hydrophobicity; mucus; nanocarriers; permeability; protein subunits; soy protein isolate; Show all 14 Subjects
Abstract:
... Modulating the size and surface charge of nanocarriers provides an efficacious strategy to enhance bioavailability of encapsulated cargos through increased mucus penetration. In this study, mucus-permHeable soy protein nanoparticles (SPNPs) were successfully fabricated via gastrointestinal proteolysis coupled with heating and pH-shifting treatment. Results showed that treatment at 65 °C and 75 °C ...
... Yellow cultivars of Flammulina filiformis are prone to browning, which causes significant yearly loss. This study investigated the critical factors involved and revealed that oxygen plays an essential role in the browning process. Integrated multi-omic analyses, including genome sequencing, transcriptome profiling, and 4D label-free proteome quantification, were incorporated to dissect essential g ...
Dušanka A. Popović Minić; Danijel D. Milinčić; Stefan Kolašinac; Vladislav Rac; Jovana Petrović; Marina Soković; Nebojša Banjac; Jelena Lađarević; Bojana B. Vidović; Aleksandar Ž. Kostić; Vladimir B. Pavlović; Mirjana B. Pešić
... This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean par ...
... During the coronavirus disease 2019 (COVID-19) pandemic, wastewater-based epidemiology (WBE) attracted attention as an objective and comprehensive indicator of community infection that does not require individual inspection. Although several severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection methods from wastewater have been developed, there are obstacles to their social implem ...
Moha Esmaeili; Chris G. Carter; Richard Wilson; Seumas P. Walker; Matthew R. Miller; Andrew R. Bridle; Tim Young; Andrea C. Alfaro; Olivier Laroche; Jane E. Symonds
proteolysis, etc ; Oncorhynchus tshawytscha; amino acid metabolism; aquaculture; blood plasma; catabolism; data collection; feed conversion; fish; genes; glucose; glutathione; isoleucine; leucine; liver; metabolites; metabolomics; multiomics; muscles; phenotype; phenylalanine; protein degradation; protein synthesis; proteomics; pyruvic acid; seawater; tryptophan; tyrosine; valine; Show all 29 Subjects
Abstract:
... Feed efficiency (FE) is a critical phenotype for aquaculture sustainability, yet it is poorly understood at the molecular level. In this study, the molecular basis of FE was investigated using a multi-omics approach to integrate proteome-level data and metabolites associated with differences in feed efficiency. To achieve this, Chinook salmon (Oncorhynchus tshawytscha) were reared in seawater and ...
... The muscle quality of farmed fish has attracted widespread public attention. To understand a comprehensive molecular mechanism underlying the effects of artificial formula feed- (AF) and grass-feeding (GF) on muscle quality and growth of grass carp (Ctenopharyngodon idellus), muscular transcriptomics and proteomics of GF and AF C. idellus were characterized. A total of 834 DEGs (484 up-, 350 down- ...
proteolysis, etc ; ligands; ligases; protein degradation; Show all 4 Subjects
Abstract:
... Targeted protein degradation (TPD) strategies have revolutionized how scientists tackle challenging protein targets deemed undruggable with traditional small molecule inhibitors. Many promising campaigns to inhibit proteins have failed due to factors surrounding inhibition selectivity and targeting of compounds to specific tissues and cell types. One of the major improvements that PROTAC (proteoly ...
proteolysis, etc ; cytotoxicity; ligases; monoclonal antibodies; Show all 4 Subjects
Abstract:
... Degrader-antibody conjugates (DACs) are novel entities that combine a proteolysis targeting chimera (PROTAC) payload with a monoclonal antibody via some type of chemical linker. This review provides a current summary of the DAC field. Many general aspects associated with the creation and biological performance of traditional cytotoxic antibody-drug conjugates (ADCs) are initially presented. These ...
proteolysis, etc ; biomarkers; peptides; proteinases; therapeutics; Show all 5 Subjects
Abstract:
... Proteases are excellent biomarkers for a variety of diseases, offer multiple opportunities for diagnostic applications and are valuable targets for therapy. From a chemistry-based perspective this review discusses and critiques the most recent advances in the field of substrate-based probes for the detection and analysis of proteolytic activity both in vitro and in vivo. ...
proteolysis, etc ; evolution; genetic suppression; thermodynamics; Show all 4 Subjects
Abstract:
... Thermodynamic stability represents one important constraint on protein evolution, but the molecular basis for how mutations that change stability impact fitness remains unclear. Here, we demonstrate that a prevalent global suppressor mutation in TEM β-lactamase, M182T, increases fitness by reducing proteolysis in vivo. We also show that a synthetic mutation, M182S, can act as a global suppressor a ...
proteolysis, etc ; T-lymphocytes; histone deacetylase; leukemia; Show all 4 Subjects
Abstract:
... We developed a first-in-class proteolysis targeting chimera (PROTAC) for selective degradation of histone deacetylase 8 (HDAC8). The PROTAC induced degradation of HDAC8 without affecting the levels of other HDACs in cellular assays, and inhibited the growth of T-cell leukemia Jurkat cells more potently than a conventional HDAC8 inhibitor. ...
proteolysis, etc ; biogenesis; cell cycle; centrosomes; proteome; Show all 5 Subjects
Abstract:
... Cellular processes are highly dependent on a dynamic proteome that undergoes structural and functional rearrangements to allow swift conversion between different cellular states. By inducing proteasomal degradation of inhibitory or stimulating factors, ubiquitylation is particularly well suited to trigger such transitions. One prominent example is the remodelling of the centrosome upon cell cycle ...
proteolysis, etc ; antimicrobial peptides; biotechnology; multiple drug resistance; Show all 4 Subjects
Abstract:
... Due to the alarming developing rate of multidrug-resistant bacterial pathogens, the development and modification of antimicrobial peptides (AMPs) are unprecedentedly active. Despite the fact that considerable efforts have been expended on the discovery and design strategies of AMPs, the clinical translation of peptide antibiotics remains inadequate. A large number of articles and reviews credited ...
Edgar Neri-Castro; Jason L. Strickland; Ruben Alonso Carbajal-Márquez; Jesús Zuñiga Adán; Roberto Ponce-López; Felipe Olvera-Rodríguez; Alejandro Alagón
proteolysis, etc ; Crotalus atrox; antivenoms; death; hybrids; toxicity; venoms; Show all 7 Subjects
Abstract:
... Here we report, for the first time, a natural hybrid between Crotalus atrox and C. mictlantecuhtli based on intermediate characteristics of the external morphology and venom. Morphologically, the individual had characteristics of both parent species. The hybrid's venom exhibited an intermediate composition including the presence of crotoxin which has never been documented in C. atrox but is well d ...