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1. Impact of elderly gastrointestinal alterations on gastric emptying and enzymatic hydrolysis of skim milk: An in vitro study using a dynamic stomach system

Author:
Ping Zhang; Shahid Iqbal; Renpan Deng; Xu Duan; Huaxin Han; Xiao Dong Chen; Peng Wu
Source:
Food chemistry 2023 v.402 pp. 134365
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; digestion; elderly; enzymatic hydrolysis; food chemistry; hydrolysis; models; skim milk; stomach; young adults;     Show all 10 Subjects
Abstract:
... This study aimed to evaluate gastric emptying and enzymatic hydrolysis of skim milk using a dynamic in vitro gastrointestinal system mimicking digestive conditions in young adult and elderly individuals. The gastric emptying half-time was 22.5 and 26.5 min in the young adult and elderly models, respectively. The degree of proteolysis at 120 min reached 42.3 % under adult digestion, significantly h ...
DOI:
10.1016/j.foodchem.2022.134365

2. Physicochemical and functional properties of protein isolated from herring co-products; effects of catching season, pre-sorting, and co-product combination

Author:
Eline van Berlo; Ingrid Undeland; Mehdi Abdollahi
Source:
Food chemistry 2023 v.398 pp. 133947
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; coproducts; food chemistry; gelation; gels; head; herring; lipid peroxidation; myosin; protein isolates; spring;     Show all 11 Subjects
Abstract:
... Effect of catching season (spring vs fall), pre-sorting and selective recombination of different herring filleting co-products on protein yield during valorisation using the alkaline pH-shift technology was studied. Impacts of the pre-processing conditions on lipid oxidation, rheological, structural and functional properties of the proteins were also investigated. The sorted frame fraction resulte ...
DOI:
10.1016/j.foodchem.2022.133947

3. An attempt at producing a “lactose-free” directly acidified mozzarella (high moisture type) by curd washing and pressing: A chemical and sensory study

Author:
Giuseppe Natrella; Giuseppe Gambacorta; Michele Faccia
Source:
International dairy journal 2023 v.136 pp. 105499
ISSN:
0958-6946
Subject:
proteolysis,   etc   ; acidification; beta-galactosidase; cold storage; galactose; glucose; lactose; lactose free diet; odors; taste;     Show all 10 Subjects
Abstract:
... Many lactose-free dairy products are produced using commercial lactase, but not without problems such as the sweetish taste due to the presence of free glucose and galactose. The aim of the present study was to obtain a lactose-free version of direct-acidified mozzarella without using the enzyme, but removing lactose by curd washing and pressing. The best results were obtained with double curd was ...
DOI:
10.1016/j.idairyj.2022.105499

4. Regulation on the quality of yogurt by phenolic fraction of mulberry pomace supplemented before and after fermentation

Author:
Huaxin Du; Xuping Wang; Huaigu Yang; Fan Zhu; Jie Liu; Jingrong Cheng; Yaosheng Lin; Daobang Tang; Xueming Liu
Source:
Food control 2023 v.144 pp. 109333
ISSN:
0956-7135
Subject:
proteolysis,   etc   ; anthocyanins; fermentation; food safety; lactic acid; mulberries; pH; particle size; peptides; pomace; proanthocyanidins; yogurt;     Show all 12 Subjects
Abstract:
... The effects of phenolic fraction of mulberry pomace on yogurt quality were investigated. Polyphenol-enriched extract of mulberry pomace (MPPE), rich in phenols, anthocyanins and proanthocyanidins, was incorporated to yogurt before and after fermentation at 0.50%, 0.75%, 1.00% and 1.25% (w/v), respectively. The addition either before or after fermentation decreased L* and b* values, increased a* va ...
DOI:
10.1016/j.foodcont.2022.109333

5. Evaluation and selection of yeasts as potential aroma enhancers for the production of dry-cured ham

Author:
Xiaohui Gong; Ruifang Mi; Xi Chen; Qiujin Zhu; Suyue Xiong; Biao Qi; Shouwei Wang
Source:
Food science and human wellness 2023 v.12 no.1 pp. 324-335
ISSN:
2213-4530
Subject:
proteolysis,   etc   ; Candida zeylanoides; Debaryomyces hansenii; Yarrowia; cured meats; flavor; ham; humans; meat protein; milk proteins; odors; tributyrin; yeasts;     Show all 13 Subjects
Abstract:
... Yeasts play a critical role in the flavor formation of dry-cured ham. In this study, 41 yeast isolates from the dry-cured ham at different processing stages were evaluated for their technological properties. Debaryomyces hansenii was the most dominant yeast and has been detected at each phase of dry-cured ham, followed by Candida zeylanoides which was mainly detected in salting phase. Yarrowia bub ...
DOI:
10.1016/j.fshw.2022.07.022

6. Examination of β-lactoglobulin-ferulic acid complexation at elevated temperature using biochemical spectroscopy, proteomics and molecular dynamics

Author:
Kourosh Abdollahi; Lloyd Condict; Andrew Hung; Stefan Kasapis
Source:
Food hydrocolloids 2023 v.134 pp. 108053
ISSN:
0268-005X
Subject:
proteolysis,   etc   ; absorption; chemical bonding; circular dichroism spectroscopy; ferulic acid; heat treatment; hydrocolloids; ligands; lysine; molecular dynamics; pH; proteomics; temperature;     Show all 13 Subjects
Abstract:
... The molecular nature of interactions between ferulic acid and β-lactoglobulin was investigated following exposure of solutions to high temperature (121 °C) at near neutral pH (7.3) using biochemical spectroscopy, proteomics and molecular dynamics. Circular dichroism (CD) and infrared spectroscopy (FTIR) argue for alterations in the secondary structure of the heated protein molecule in the presence ...
DOI:
10.1016/j.foodhyd.2022.108053

7. Formation of mucus-permeable nanoparticles from soy protein isolate by partial enzymatic hydrolysis coupled with thermal and pH-shifting treatment

Author:
Dan Yuan; Feibai Zhou; Zhicheng Niu; Penghui Shen; Mouming Zhao
Source:
Food chemistry 2023 v.398 pp. 133851
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; bioavailability; crosslinking; disulfide bonds; encapsulation; enzymatic hydrolysis; food chemistry; gastrointestinal system; hydrophobicity; mucus; nanocarriers; permeability; protein subunits; soy protein isolate;     Show all 14 Subjects
Abstract:
... Modulating the size and surface charge of nanocarriers provides an efficacious strategy to enhance bioavailability of encapsulated cargos through increased mucus penetration. In this study, mucus-permHeable soy protein nanoparticles (SPNPs) were successfully fabricated via gastrointestinal proteolysis coupled with heating and pH-shifting treatment. Results showed that treatment at 65 °C and 75 °C ...
DOI:
10.1016/j.foodchem.2022.133851

8. Integrated multi-omic analyses on yellow Flammulina filiformis cultivar reveal postharvest oxidative damage responses

Author:
Yu Fu; Hao Tan; Bo Wang; Weihong Peng; Qun Sun; Yang Yu
Source:
Postharvest biology and technology 2023 v.195 pp. 112111
ISSN:
0925-5214
Subject:
proteolysis,   etc   ; DNA; Flammulina; biosynthesis; catechol oxidase; cell senescence; cultivars; genome; laccase; melanin; mushrooms; oxidative phosphorylation; oxygen; proteome; proteomics; technology; transcriptome; transcriptomics; tyrosine;     Show all 19 Subjects
Abstract:
... Yellow cultivars of Flammulina filiformis are prone to browning, which causes significant yearly loss. This study investigated the critical factors involved and revealed that oxygen plays an essential role in the browning process. Integrated multi-omic analyses, including genome sequencing, transcriptome profiling, and 4D label-free proteome quantification, were incorporated to dissect essential g ...
DOI:
10.1016/j.postharvbio.2022.112111

9. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization

Author:
Dušanka A. Popović Minić; Danijel D. Milinčić; Stefan Kolašinac; Vladislav Rac; Jovana Petrović; Marina Soković; Nebojša Banjac; Jelena Lađarević; Bojana B. Vidović; Aleksandar Ž. Kostić; Vladimir B. Pavlović; Mirjana B. Pešić
Source:
Food chemistry 2023 v.402 pp. 134299
ISSN:
0308-8146
Subject:
proteolysis,   etc   ; Agaricus subrufescens; Fourier transform infrared spectroscopy; electron microscopy; electrophoresis; food chemistry; glycosides; goat milk; hydrogen; hydrophobicity; microstructure; milk; milk proteins; particle size; polypeptides; polysaccharides;     Show all 16 Subjects
Abstract:
... This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean par ...
DOI:
10.1016/j.foodchem.2022.134299

10. COPMAN: A novel high-throughput and highly sensitive method to detect viral nucleic acids including SARS-CoV-2 RNA in wastewater

Author:
Yuka Adachi Katayama; Shin Hayase; Yoshinori Ando; Tomohiro Kuroita; Kazuya Okada; Ryo Iwamoto; Toru Yanagimoto; Masaaki Kitajima; Yusaku Masago
Source:
Science of the total environment 2023 v.856 pp. 158966
ISSN:
0048-9697
Subject:
proteolysis,   etc   ; COVID-19 infection; Japan; Pepper mild mottle virus; RNA; Severe acute respiratory syndrome coronavirus 2; automation; chlorides; coagulation; environment; feces; magnetism; pandemic; people; rivers; viruses; wastewater;     Show all 17 Subjects
Abstract:
... During the coronavirus disease 2019 (COVID-19) pandemic, wastewater-based epidemiology (WBE) attracted attention as an objective and comprehensive indicator of community infection that does not require individual inspection. Although several severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection methods from wastewater have been developed, there are obstacles to their social implem ...
DOI:
10.1016/j.scitotenv.2022.158966

11. An integrated proteomics and metabolomics investigation of feed efficiency in seawater reared Chinook salmon (Oncorhynchus tshawytscha)

Author:
Moha Esmaeili; Chris G. Carter; Richard Wilson; Seumas P. Walker; Matthew R. Miller; Andrew R. Bridle; Tim Young; Andrea C. Alfaro; Olivier Laroche; Jane E. Symonds
Source:
Aquaculture 2023 v.562 pp. 738845
ISSN:
0044-8486
Subject:
proteolysis,   etc   ; Oncorhynchus tshawytscha; amino acid metabolism; aquaculture; blood plasma; catabolism; data collection; feed conversion; fish; genes; glucose; glutathione; isoleucine; leucine; liver; metabolites; metabolomics; multiomics; muscles; phenotype; phenylalanine; protein degradation; protein synthesis; proteomics; pyruvic acid; seawater; tryptophan; tyrosine; valine;     Show all 29 Subjects
Abstract:
... Feed efficiency (FE) is a critical phenotype for aquaculture sustainability, yet it is poorly understood at the molecular level. In this study, the molecular basis of FE was investigated using a multi-omics approach to integrate proteome-level data and metabolites associated with differences in feed efficiency. To achieve this, Chinook salmon (Oncorhynchus tshawytscha) were reared in seawater and ...
DOI:
10.1016/j.aquaculture.2022.738845

12. Integrated multiple-omics reveals the regulatory mechanism underlying the effects of artificial feed and grass feeding on growth and muscle quality of grass carp (Ctenopharyngodon idellus)

Author:
Honghao Zhao; Jasmine Chong; Dapeng Li; Jianguo Xia
Source:
Aquaculture 2023 v.562 pp. 738808
ISSN:
0044-8486
Subject:
proteolysis,   etc   ; Ctenopharyngodon idella; aquaculture; beta oxidation; cell proliferation; droplets; farmed fish; flavor; glycogen; glycolysis; grasses; hypertrophy; insulin resistance; lipids; muscle fibers; muscles; necroptosis; peroxisome proliferator-activated receptor alpha; phosphatidylinositol 3-kinase; protein synthesis; proteomics; stress tolerance; texture; transcriptomics; tricarboxylic acid cycle; ubiquitin;     Show all 26 Subjects
Abstract:
... The muscle quality of farmed fish has attracted widespread public attention. To understand a comprehensive molecular mechanism underlying the effects of artificial formula feed- (AF) and grass-feeding (GF) on muscle quality and growth of grass carp (Ctenopharyngodon idellus), muscular transcriptomics and proteomics of GF and AF C. idellus were characterized. A total of 834 DEGs (484 up-, 350 down- ...
DOI:
10.1016/j.aquaculture.2022.738808

13. Target and tissue selectivity of PROTAC degraders

Author:
Robert G. Guenette; Seung Wook Yang; Jaeki Min; Baikang Pei; Patrick Ryan Potts
Source:
Chemical Society reviews 2022 v.51 no.14 pp. 5740-5756
ISSN:
1460-4744
Subject:
proteolysis,   etc   ; ligands; ligases; protein degradation;     Show all 4 Subjects
Abstract:
... Targeted protein degradation (TPD) strategies have revolutionized how scientists tackle challenging protein targets deemed undruggable with traditional small molecule inhibitors. Many promising campaigns to inhibit proteins have failed due to factors surrounding inhibition selectivity and targeting of compounds to specific tissues and cell types. One of the major improvements that PROTAC (proteoly ...
DOI:
10.1039/d2cs00200k

14. Degrader-antibody conjugates

Author:
Peter S. Dragovich
Source:
Chemical Society reviews 2022 v.51 no.10 pp. 3886-3897
ISSN:
1460-4744
Subject:
proteolysis,   etc   ; cytotoxicity; ligases; monoclonal antibodies;     Show all 4 Subjects
Abstract:
... Degrader-antibody conjugates (DACs) are novel entities that combine a proteolysis targeting chimera (PROTAC) payload with a monoclonal antibody via some type of chemical linker. This review provides a current summary of the DAC field. Many general aspects associated with the creation and biological performance of traditional cytotoxic antibody-drug conjugates (ADCs) are initially presented. These ...
DOI:
10.1039/d2cs00141a

15. Peptide probes for proteases – innovations and applications for monitoring proteolytic activity

Author:
Maria Rodriguez-Rios; Alicia Megia-Fernandez; Daniel J. Norman; Mark Bradley
Source:
Chemical Society reviews 2022 v.51 no.6 pp. 2081-2120
ISSN:
1460-4744
Subject:
proteolysis,   etc   ; biomarkers; peptides; proteinases; therapeutics;     Show all 5 Subjects
Abstract:
... Proteases are excellent biomarkers for a variety of diseases, offer multiple opportunities for diagnostic applications and are valuable targets for therapy. From a chemistry-based perspective this review discusses and critiques the most recent advances in the field of substrate-based probes for the detection and analysis of proteolytic activity both in vitro and in vivo. ...
DOI:
10.1039/d1cs00798j

16. Natural and Synthetic Suppressor Mutations Defy Stability–Activity Tradeoffs

Author:
Sonya Lee; Cynthia N. Okoye; Devin Biesbrock; Emily C. Harris; Katelyn F. Miyasaki; Ryan G. Rilinger; Megalan Tso; Kathryn M. Hart
Source:
Biochemistry 2022 v.61 no.5 pp. 398-407
ISSN:
1520-4995
Subject:
proteolysis,   etc   ; evolution; genetic suppression; thermodynamics;     Show all 4 Subjects
Abstract:
... Thermodynamic stability represents one important constraint on protein evolution, but the molecular basis for how mutations that change stability impact fitness remains unclear. Here, we demonstrate that a prevalent global suppressor mutation in TEM β-lactamase, M182T, increases fitness by reducing proteolysis in vivo. We also show that a synthetic mutation, M182S, can act as a global suppressor a ...
DOI:
10.1021/acs.biochem.1c00805

17. Selective degradation of histone deacetylase 8 mediated by a proteolysis targeting chimera (PROTAC)

Author:
Jiranan Chotitumnavee; Yasunobu Yamashita; Yukari Takahashi; Yuri Takada; Tetsuya Iida; Makoto Oba; Yukihiro Itoh; Takayoshi Suzuki
Source:
Chemical communications 2022 v.58 no.29 pp. 4635-4638
ISSN:
1364-548X
Subject:
proteolysis,   etc   ; T-lymphocytes; histone deacetylase; leukemia;     Show all 4 Subjects
Abstract:
... We developed a first-in-class proteolysis targeting chimera (PROTAC) for selective degradation of histone deacetylase 8 (HDAC8). The PROTAC induced degradation of HDAC8 without affecting the levels of other HDACs in cellular assays, and inhibited the growth of T-cell leukemia Jurkat cells more potently than a conventional HDAC8 inhibitor. ...
DOI:
10.1039/d2cc00272h

18. Proteolytic control in ciliogenesis: Temporal restriction or early initiation?

Author:
Gregor Habeck; Jörg Schweiggert
Source:
BioEssays 2022 v.44 no.9 pp. e2200087
ISSN:
0265-9247
Subject:
proteolysis,   etc   ; biogenesis; cell cycle; centrosomes; proteome;     Show all 5 Subjects
Abstract:
... Cellular processes are highly dependent on a dynamic proteome that undergoes structural and functional rearrangements to allow swift conversion between different cellular states. By inducing proteasomal degradation of inhibitory or stimulating factors, ubiquitylation is particularly well suited to trigger such transitions. One prominent example is the remodelling of the centrosome upon cell cycle ...
DOI:
10.1002/bies.202200087

19. Strategies employed in the design of antimicrobial peptides with enhanced proteolytic stability

Author:
Zhenheng Lai; Xiaojie Yuan; Hongyu Chen; Yunhui Zhu; Na Dong; Anshan Shan
Source:
Biotechnology advances 2022 v.59 pp. 107962
ISSN:
0734-9750
Subject:
proteolysis,   etc   ; antimicrobial peptides; biotechnology; multiple drug resistance;     Show all 4 Subjects
Abstract:
... Due to the alarming developing rate of multidrug-resistant bacterial pathogens, the development and modification of antimicrobial peptides (AMPs) are unprecedentedly active. Despite the fact that considerable efforts have been expended on the discovery and design strategies of AMPs, the clinical translation of peptide antibiotics remains inadequate. A large number of articles and reviews credited ...
DOI:
10.1016/j.biotechadv.2022.107962

20. Characterization of the venom and external morphology of a natural hybrid between Crotalus atrox and Crotalus mictlantecuhtli

Author:
Edgar Neri-Castro; Jason L. Strickland; Ruben Alonso Carbajal-Márquez; Jesús Zuñiga Adán; Roberto Ponce-López; Felipe Olvera-Rodríguez; Alejandro Alagón
Source:
Toxicon 2022 v.207 pp. 43-47
ISSN:
0041-0101
Subject:
proteolysis,   etc   ; Crotalus atrox; antivenoms; death; hybrids; toxicity; venoms;     Show all 7 Subjects
Abstract:
... Here we report, for the first time, a natural hybrid between Crotalus atrox and C. mictlantecuhtli based on intermediate characteristics of the external morphology and venom. Morphologically, the individual had characteristics of both parent species. The hybrid's venom exhibited an intermediate composition including the presence of crotoxin which has never been documented in C. atrox but is well d ...
DOI:
10.1016/j.toxicon.2022.01.003