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1. Effects of different carbon sources on the removal of ciprofloxacin and pollutants by activated sludge: Mechanism and biodegradation

Author:
Cai, Yixiang; Yan, Zhiyong; Ou, Yingjuan; Peng, Boshang; Zhang, Lihua; Shao, Jihai; Lin, Yiqing; Zhang, Jiachao
Source:
Journal of environmental sciences (China) 2022 v.111 pp. 240-248
ISSN:
1001-0742
Subject:
quantitative polymerase chain reaction,   etc   ; Acidobacteria; Microbacterium; Rhodanobacter; activated sludge; biodegradation; carbon; ciprofloxacin; denitrification; glucose; nitrates; nitrification; nitrites; sodium acetate; wastewater; China;     Show all 16 Subjects
Abstract:
... This research investigated the effects of ciprofloxacin (CIP) (0.5, 5, and 20 mg/L) on SBR systems under different carbon source conditions. Microbial community abundance and structure were determined by quantitative PCR and high-throughput sequencing, respectively. The biodegradation production of CIP and possible degradation mechanism were also studied. Results showed that CIP had adverse effect ...
DOI:
10.1016/j.jes.2021.03.037

2. Reference gene selection for miRNA and mRNA normalization in potato in response to potato virus Y

Author:
Yin, Zhimin; Xie, Fuliang; Michalak, Krystyna; Zhang, Baohong; Zimnoch-Guzowska, Ewa
Source:
Molecular and cellular probes 2021 v.55 pp. 101691
ISSN:
0890-8508
Subject:
quantitative polymerase chain reaction,   etc   ; Potato virus Y; genes; microRNA; potatoes;     Show all 5 Subjects
Abstract:
... This was the first report on evaluating candidate reference genes for quantifying the expression profiles of both coding (e.g., mRNA) and non-coding (e.g., miRNA) genes in potato response to potato virus Y (PVY) inoculation. The reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) method was employed to quantify the expression profiles of eight selected candidate refere ...
DOI:
10.1016/j.mcp.2020.101691

3. Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification

Author:
Tu, Jing; Qiao, Yi; Luo, Yuhan; Long, Naiyun; Lu, Zuhong
Source:
RSC advances 2021 v.11 no.8 pp. 4617-4621
ISSN:
2046-2069
Subject:
quantitative polymerase chain reaction,   etc   ; DNA; detection limit; fluorescence; genome;     Show all 5 Subjects
Abstract:
... DNA quantification is important in the research of life sciences. In an independent quantification process, the extracted part of a DNA sample is usually difficult to be recycled for further use while the widely used real-time PCR is used to count the copies with certain sequences. Based on the popular multiple displacement amplification (MDA), we proposed and performed quantitative real-time MDA ...
DOI:
10.1039/d0ra09021b

4. Evaluation of NOx removal from flue gas and Fe(II)EDTA regeneration using a novel BTF–ABR integrated system

Author:
Wang, Yanling; Li, Jianjun; Huang, Shaobin; Huang, Xingzhu; Hu, Wenzhe; Pu, Jia; Xu, Meiying
Source:
Journal of hazardous materials 2021 v.415 pp. 125741
ISSN:
0304-3894
Subject:
quantitative polymerase chain reaction,   etc   ; Geobacter; Klebsiella; denitrification; flue gas; genes; nitrates; nitrites;     Show all 8 Subjects
Abstract:
... A promising process is under development for the removal of NOx and regeneration of Fe(II)EDTA in a novel biotrickling filter–anaerobic baffled reactor (BTF–ABR) integrated system at 50 ± 0.5 ℃. In this work, we investigated the NOx removal capacity of a BTF under different O₂ concentrations (7.0 vol%, 5.25 vol% and 3.5 vol%), and tested the effect of an ABR on NOx removal and regeneration of Fe(I ...
DOI:
10.1016/j.jhazmat.2021.125741

5. In situ analysis of antibiotic resistance genes in anaerobically digested dairy manure and its subsequent disposal facilities

Author:
Lu, Yi; Sun, Renhua; Zhang, Changai; Ding, Shaohua; Ying, Mengfei; Shan, Shengdao
Source:
Bioresource technology 2021 v.333 pp. 124988
ISSN:
0960-8524
Subject:
quantitative polymerase chain reaction,   etc   ; Clostridium difficile; anaerobic digestion; antibiotic resistance; dairy manure; metagenomics; plasmids; slurries; soil;     Show all 9 Subjects
Abstract:
... The metagenomic and quantitative polymerase chain reaction approaches were combined to evaluate the profiles of ARGs and plasmids in anaerobically digested dairy manure in situ and reveal the persistence and elevation of typical ARGs and plasmids in its subsequent disposal facilities in CAFOs, respectively. Our results indicated that the typical ARGs and plasimd were mainly sul2, mefa, tetm-01, te ...
DOI:
10.1016/j.biortech.2021.124988

6. A real-time PCR method to assess the presence of vertebrate material in plant-based products

Author:
Cottenet, Geoffrey; Blancpain, Carine
Source:
Food control 2021 v.125 pp. 108001
ISSN:
0956-7135
Subject:
quantitative polymerase chain reaction,   etc   ; commercialization; fish; food safety; genes; laws and regulations; meat; mitochondria; vegan diet;     Show all 9 Subjects
Abstract:
... Consumption of plant-based products and meat alternatives has been rising for a few years, with vegetarian and vegan diets gaining more and more popularity. To comply with legislation and maintain consumers’ trust, food manufacturers of vegan products must ensure the absence of animal residues in these products. A real-time PCR method targeting the 16S mitochondrial rRNA gene from vertebrates has ...
DOI:
10.1016/j.foodcont.2021.108001

7. Heavy metal could drive co-selection of antibiotic resistance in terrestrial subsurface soils

Author:
Wang, Xiaomin; Lan, Bangrui; Fei, Hexin; Wang, Shanyun; Zhu, Guibing
Source:
Journal of hazardous materials 2021 v.411 pp. 124848
ISSN:
0304-3894
Subject:
quantitative polymerase chain reaction,   etc   ; antibiotic resistance; aquifers; heavy metals; metagenomics; metal tolerance; pollution; spatial variation;     Show all 8 Subjects
Abstract:
... Terrestrial surface ecosystems are important sinks for antibiotic resistance genes (ARGs) due to the continuous discharge of contaminants from human-impacted ecosystems. However, the abundance and resistance types of ARGs and their influencing factors in terrestrial subsurface soils are not well known. In this study, we investigated the abundance and diversity of ARGs, and their correlations with ...
DOI:
10.1016/j.jhazmat.2020.124848

8. CircRNA circBACH1 (hsa_circ_0061395) serves as a miR-656–3p sponge to facilitate hepatocellular carcinoma progression through increasing SERBP1 expression

Author:
Li, GuoYong; Du, Peng; He, JianPeng; Li, Yong
Source:
Biochemical and biophysical research communications 2021 v.556 pp. 1-8
ISSN:
0006-291X
Subject:
quantitative polymerase chain reaction,   etc   ; apoptosis; cadherins; carcinogenicity; flow cytometry; hepatoma; microRNA; research; vimentin; xenotransplantation;     Show all 10 Subjects
Abstract:
... Hsa_circ_0061395(circBACH1) and SERBP1(SERPINE1 mRNA binding protein 1) have been reported to play a carcinogenic role in HCC.In this study, circBACH1, microRNA(miR)-656–3p, and SERBP1 expression levels with quantitative real-time polymerase chain reaction (qRT-PCR) in HCC tissue specimens and cells.The protein levels of SERBP1, E-Cadherin, vimentin, and N-Cadherin were detected with western blott ...
DOI:
10.1016/j.bbrc.2021.03.136

9. Modulation of the inflammatory environment by spermatozoa through regulation of transforming growth factor beta in porcine uterine epithelial cells

Author:
Kim, Su-Jin; Hwangbo, Yong; Park, Choon-Keun
Source:
Reproductive Biology 2021 v.21 no.2 pp. 100484
ISSN:
1642-431X
Subject:
quantitative polymerase chain reaction,   etc   ; boars; epithelium; gene expression; inflammation; interleukin-6; interleukin-8; plasminogen; spermatozoa; uterus;     Show all 10 Subjects
Abstract:
... This study investigated the changes in the mRNA expression of transforming growth factor beta (TGF-β), plasminogen activators (PAs), and interleukin (IL) caused by sperm, as well as the regulatory mechanism of PA activity through TGF-β, in porcine uterine epithelial cells. The cells were isolated from the uterine horn of pig and co-incubated with Percoll-separated boar sperm (45% or 90%), or TGF-β ...
DOI:
10.1016/j.repbio.2021.100484

10. New sensitive methods for fraud detection in buffalo dairy products

Author:
Azevedo, Bianca Tainá; Vercesi Filho, Anibal Eugênio; Gutmanis, Gunta; Verissimo, Cecília José; Katiki, Luciana Morita; Okino, Cintia Hiromi; Cristina de Sena Oliveira, Márcia; Giglioti, Rodrigo
Source:
International dairy journal 2021 v.117 pp. 105013
ISSN:
0958-6946
Subject:
quantitative polymerase chain reaction,   etc   ; DNA; adulterated products; buffalo milk; buffaloes; fraud; manufacturing; milk;     Show all 8 Subjects
Abstract:
... The rising consumption of buffalo milk derivatives in recent years has highlighted the search for buffaloes in Brazilian livestock farming. These products have greater added value compared with common dairy milk products. Consequently, addition of variable amounts of cows' milk during manufacture of buffalo dairy products may occur, which constitutes fraud by product adulteration. Thus, the presen ...
DOI:
10.1016/j.idairyj.2021.105013

11. Rapid and absolute quantification of VBNC Cronobacter sakazakii by PMAxx combined with single intact cell droplet digital PCR in infant foods

Author:
Lv, Xinrui; Gu, Xiaokui; Wang, Li; He, Xiaoxin; He, Chunai; Zhang, Jingfeng; Zhao, Lichao
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.145 pp. 111388
ISSN:
0023-6438
Subject:
quantitative polymerase chain reaction,   etc   ; Cronobacter sakazakii; detection limit; droplets; food safety; infant foods; propidium; temperature;     Show all 8 Subjects
Abstract:
... Cronobacter sakazakii (C. sakazakii) in viable but nonculturable (VBNC) state might evade traditional culture detection, and pose a serious threat to food safety. The objective of this study was to establish an improved propidium monoazide (PMAxx), combined with a single intact cell droplet digital PCR (SIC ddPCR) method for rapid and absolute quantification of VBNC C. sakazakii and apply it to th ...
DOI:
10.1016/j.lwt.2021.111388

12. Simultaneous enumeration of Cronobacter sakazakii and Staphylococcus aureus in powdered infant foods through duplex TaqMan real-time PCR

Author:
Xie, Xueqin; Liu, Zhou
Source:
International dairy journal 2021 v.117 pp. 105019
ISSN:
0958-6946
Subject:
quantitative polymerase chain reaction,   etc   ; Cronobacter sakazakii; Staphylococcus aureus; fluorescence; operon; pathogens; plasmids; thermal stability;     Show all 8 Subjects
Abstract:
... Cronobacter sakazakii and Staphylococcus aureus are important pathogens. Targeting the species-specific macromolecular synthesis (MMS) operon and thermostable nuclease gene (nuc), a duplex TaqMan real-time PCR (dRT-PCR) assay was developed for their simultaneous enumeration within two kinds of powdered infant foods (PIFs). The assay was highly specific, producing fluorescent signals exclusively fr ...
DOI:
10.1016/j.idairyj.2021.105019

13. Inhibition of MTA2 and MTA3 induces mesendoderm specification of human embryonic stem cells

Author:
You, Yu; Cui, Yandi; Li, Yuting; Zhang, Meng; Wang, Xin; Ji, Junxiang; Zhang, Xinbao; Zhou, Min; Zhang, Zhonglin; Ye, Shou-Dong; Wang, Xiaoxiao
Source:
Biochemical and biophysical research communications 2021 v.552 pp. 142-149
ISSN:
0006-291X
Subject:
quantitative polymerase chain reaction,   etc   ; humans; messenger RNA; phenotype; phosphotransferases (kinases); research; tyrosine;     Show all 7 Subjects
Abstract:
... Fully understanding the regulatory network under the pluripotency of embryonic stem cells (ESC) is a prerequisite for their safe application. Here, we addressed the characteristics of metastasis-associated (MTA) family members in human ESCs and found that knockdown of the expression of MTA2 and MTA3, but not MTA1, would induce differentiation. High-throughput sequence and quantitative real-time PC ...
DOI:
10.1016/j.bbrc.2021.03.030

14. Abundance of tigecycline resistance genes and association with antibiotic residues in Chinese livestock farms

Author:
Fu, Yulin; Chen, Yiqiang; Liu, Dejun; Yang, Dawei; Liu, Zhihai; Wang, Yingyu; Wang, Jiayi; Wang, Xueyang; Xu, Xiangyue; Li, Xing; He, Junjia; Jiang, Junyao; Zhai, Weishuai; Huang, Lingli; He, Tao; Xia, Xi; Cai, Chang; Wang, Yang; Jiang, Haiyang
Source:
Journal of hazardous materials 2021 v.409 pp. 124921
ISSN:
0304-3894
Subject:
quantitative polymerase chain reaction,   etc   ; ; cattle; chickens; feces; florfenicol; public health; risk; swine; tiamulin; tigecycline; transporters;     Show all 12 Subjects
Abstract:
... The discovery of plasmid-mediated tet(X) variants and efflux pump gene tmexCD1-toprJ1 conferring bacteria resistance to tigecycline has compromised glycylcycline as the last line of defense against infection, which poses serious threat to public health. Herein, real-time quantitative PCR was used to detect the abundance of seven tigecycline resistance genes (TRGs), including six tet(X) variants an ...
DOI:
10.1016/j.jhazmat.2020.124921

15. Solar photon-Fenton process eliminates free plasmid DNA harboring antimicrobial resistance genes from wastewater

Author:
Vilela, Pâmela B.; Martins, Alessandra S.; Starling, Maria Clara V.M.; de Souza, Felipe A.R.; Pires, Giovana F.F.; Aguilar, Ananda P.; Pinto, Maria Eduarda A.; Mendes, Tiago A.O.; de Amorim, Camila C.
Source:
Journal of environmental management 2021 v.285 pp. 112204
ISSN:
0301-4797
Subject:
quantitative polymerase chain reaction,   etc   ; Escherichia coli; ampicillin; antibiotic resistance; kanamycin; municipal wastewater; pH; plasmids; wastewater treatment;     Show all 9 Subjects
Abstract:
... This work aimed to assess the elimination and inactivation of resistance-conferring plasmids (RCPs) present in suspension in secondary wastewater by solar photo-Fenton as these are important vectors for the dissemination of antimicrobial resistance. Experiments were performed in synthetic secondary wastewater (SWW) and municipal wastewater treatment plant effluent (MWWTPE). Solar photo-Fenton (50  ...
DOI:
10.1016/j.jenvman.2021.112204

16. Application of comparative genomics in the development of DNA probes to detect Bacillus cereus and Bacillus subtilis

Author:
Kwon, Eun-Ae; Lee, Jae-Ik; Park, Jeong Woong; Kim, Sang-Soon
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.142 pp. 110996
ISSN:
0023-6438
Subject:
quantitative polymerase chain reaction,   etc   ; Bacillus cereus; Bacillus subtilis; DNA; databases; genome; genomics; milk;     Show all 8 Subjects
Abstract:
... Primer-probe sets to detect Bacillus cereus and/or B. subtilis in food products were developed based on panX (a pan-genome analysis tool). Data for 60 B. cereus strains and 131 B. subtilis strains retrieved from the NCBI RefSeq database were analyzed by panX. Primer-probe sets for B. cereus (ccpA) and B. subtilis (cotQ) were developed by comparing the core genomes of the two bacteria. The develope ...
DOI:
10.1016/j.lwt.2021.110996

17. Comparative profile of exosomal microRNAs in postmenopausal women with various bone mineral densities by small RNA sequencing

Author:
Kong, Dece; Chen, Tianning; Zheng, Xinhui; Yang, Tieyi; Zhang, Yan; Shao, Jin
Source:
Genomics 2021 v.113 no.3 pp. 1514-1521
ISSN:
0888-7543
Subject:
quantitative polymerase chain reaction,   etc   ; bone density; exosomes; gene ontology; genomics; microRNA; osteopenia; osteoporosis; postmenopause; prediction;     Show all 10 Subjects
Abstract:
... To explore the role of plasma miRNAs in exosomes in early postmenopausal women. Small RNA sequencing was implemented to clarify the expression of miRNA in plasma exosomes obtained from 15 postmenopausal women, divided into groups of osteoporosis, osteopenia, and normal bone mass based on bone mineral density. Differentially expressed miRNAs (DEMs) were identified by comparing miRNA expression prof ...
DOI:
10.1016/j.ygeno.2021.03.028

18. Genetic diversity of porcine circovirus 3 strains and the first detection of two different PCV3 strains coinfecting the same host in Minas Gerais, Brazil

Author:
Assao, Viviane Sisdelli; Santos, Marcus Rebouças; Rosado, Nívia Carolina Lopes; Bressan, Gustavo Costa; Fietto, Juliana Lopes Rangel; Chang, Yung-Fu; Vidigal, Pedro Marcus Pereira; Silva-Júnior, Abelardo
Source:
Archives of virology 2021 v.166 no.5 pp. 1463-1468
ISSN:
0304-8608
Subject:
quantitative polymerase chain reaction,   etc   ; Porcine circovirus-3; genetic variation; mixed infection; pork industry; virology; Brazil;     Show all 7 Subjects
Abstract:
... Porcine circovirus 3 (PCV3) is a recently emerged circovirus discovered in 2016 that has drawn the attention of the swine industry worldwide. In this study, we evaluated the genetic diversity of PCV3 strains on pig farms. A total of 261 samples from sows, weaning pigs, growing pigs, and stillborn/mummified fetuses were analyzed by quantitative real-time PCR. The results revealed that at least two ...
DOI:
10.1007/s00705-021-05032-y

19. Specific detection of the most prevalent five Listeria strains and unspecific detection of 15 Listeria using multiplex real-time PCR

Author:
Köppel, René; Schade, Jasmin; Peier, Martin
Source:
European food research & technology 2021 v.247 no.5 pp. 1167-1175
ISSN:
1438-2377
Subject:
quantitative polymerase chain reaction,   etc   ; DNA; Listeria monocytogenes; food research; risk; technology;     Show all 6 Subjects
Abstract:
... Listeria in food, are a serious risk to consumer’s health that may even have a fatal outcome. To analyze Listeria in food, the methods used must provide reliable results and detect all strains of Listeria. Several qPCR systems have been published for the identification of Listeria monocytogenes, innocua, ivanovii, welshimeri and seeligeri. PCR systems for Listeria spp. have also been published. Ho ...
DOI:
10.1007/s00217-021-03697-y

20. Precision hyperthermia-induced miRNA-409–3p upregulation inhibits migration, invasion, and EMT of gastric cancer cells by targeting KLF17

Author:
Feng, Jinxin; Li, Kejun; Liu, Gaojie; Feng, Yanlin; Shi, Huijuan; Zhang, Xiangliang
Source:
Biochemical and biophysical research communications 2021 v.549 pp. 113-119
ISSN:
0006-291X
Subject:
quantitative polymerase chain reaction,   etc   ; apoptosis; drug therapy; fever; microRNA; research; stomach neoplasms;     Show all 7 Subjects
Abstract:
... Hyperthermic intraperitoneal chemotherapy (HIPEC) is widely used for clinical treatment of advanced cancers. However, the regulatory mechanism underlying precise hyperthermia treatment in advanced gastric cancer (AGC) remains unclear. MiR-409–3p is reportedly downregulated in a variety of cancers, although its role in regulating treatment of AGC by precise hyperthermia remains unclear. The underly ...
DOI:
10.1016/j.bbrc.2021.02.063