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You searched for: Subject "quantitative polymerase chain reaction" Remove constraint Subject: "quantitative polymerase chain reaction"
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1. Co-occurrence of genes for antibiotic resistance and arsenic biotransformation in paddy soils

Author:
Huiling Cui; Dong Zhu; Longjun Ding; Yifei Wang; Jianqiang Su; Guilan Duan; Yongguan Zhu
Source:
Journal of environmental sciences (China) 2023 v.125 pp. 701-711
ISSN:
1001-0742
Subject:
quantitative polymerase chain reaction,   etc   ; Clostridium; antibiotic resistance; biotransformation; food safety; manure amendments; manure spreading; methylation; multiple drug resistance; paddies; paddy soils; pollution; risk; China;     Show all 14 Subjects
Abstract:
... Paddy soils are potential hotspots of combined contamination with arsenic (As) and antibiotics, which may induce co-selection of antibiotic resistance genes (ARGs) and As biotransformation genes (ABGs), resulting in dissemination of antimicrobial resistance and modification in As biogeochemical cycling. So far, little information is available for these co-selection processes and specific patterns ...
DOI:
10.1016/j.jes.2022.02.027

2. Mepanipyrim induces visual developmental toxicity and vision-guided behavioral alteration in zebrafish larvae

Author:
Chao Shen; Yimei Cai; Jialing Li; Chengyong He; Zhenghong Zuo
Source:
Journal of environmental sciences (China) 2023 v.124 pp. 76-88
ISSN:
1001-0742
Subject:
quantitative polymerase chain reaction,   etc   ; Danio rerio; anilinopyrimidine fungicides; aryl hydrocarbon receptors; developmental toxicity; fruit growing; fungi; genes; light intensity; mepanipyrim; opsin; phototransduction; transcriptomics; wavelengths; China;     Show all 15 Subjects
Abstract:
... Mepanipyrim, an anilinopyrimidine fungicide, has been extensively used to prevent fungal diseases in fruit culture. Currently, research on mepanipyrim-induced toxicity in organisms is still very scarce, especially visual developmental toxicity. Here, zebrafish larvae were employed to investigate mepanipyrim-induced visual developmental toxicity. Intense light and monochromatic light stimuli-evoked ...
DOI:
10.1016/j.jes.2021.11.008

3. Comparison of real‐time PCR tests for the detection of Synchytrium endobioticum resting spores in soil and their potential application in descheduling of previously infested plots

Author:
Bart T. L. H. van de Vossenberg; Naomi te Braak; Tim Warbroek; Johan P. Meffert; Karin H. M. Rosendahl‐Peters; Marga P. E. van Gent‐Pelzer; Patricia C. J. van Rijswick
Source:
Bulletin OEPP 2022 v.52 no.1 pp. 154-163
ISSN:
0250-8052
Subject:
quantitative polymerase chain reaction,   etc   ; Synchytrium endobioticum; potatoes; soil;     Show all 4 Subjects
Abstract:
... Plots infested with Synchytrium endobioticum, the causal agent of potato wart disease, are scheduled, resulting in prohibition of potato cultivation of and cultivation of plants for planting. On account of robust resting spores that are present in infested soils, plots remain scheduled for 20 years. After this period, plots are intensively sampled and the presence of resting spores in soil is dete ...
DOI:
10.1111/epp.12813

4. Optimized quantitative PCR analysis of random DNA aptamer libraries

Author:
Keenan Pearson; Caroline Doherty; Drason Zhang; Nicole A. Becker; L. James Maher
Source:
Analytical biochemistry 2022 v.650 pp. 114712
ISSN:
0003-2697
Subject:
quantitative polymerase chain reaction,   etc   ; DNA; automation; fluorescence; oligonucleotides;     Show all 5 Subjects
Abstract:
... The quantitative polymerase chain reaction (qPCR) with detection of duplex DNA yield by intercalator fluorescence is a common and essential technique in nucleic acid analysis. We encountered unexpected results when applying standard qPCR methods to the quantitation of random DNA libraries flanked by regions of fixed sequence, a configuration essential for in vitro selection experiments. Here we de ...
DOI:
10.1016/j.ab.2022.114712

5. Development of a Duplex Digital PCR Method to Quantify Five Genetically Modified Soybean Events

Author:
Likun Long; Wei Yan; Yuxuan He; Liming Dong; Zhenjuan Xing; Congcong Li; Wei Xia; Feiwu Li
Source:
Food analytical methods 2022 v.15 no.2 pp. 294-306
ISSN:
1936-9751
Subject:
quantitative polymerase chain reaction,   etc   ; commercialization; droplets; fluorescence; genes; soybeans;     Show all 6 Subjects
Abstract:
... The presence of genetically modified organisms (GMOs) in food and feed products is regulated in many countries. Various countries have issued labeling threshold levels to supervise GMO commercialization, and efficient detection methods to quantify GMO contents are critical to meet labeling regulations. The aim of this study was to develop event-specific methods to measure the event-specific/lectin ...
DOI:
10.1007/s12161-021-02104-2

6. Screening of tobacco genotypes for Phytophthora nicotianae resistance

Author:
Liu Yutong; Sun Mingming; Jiang Zipeng; Wang Xinfang; Xiao Bingguang; Yang Aiguo; Meng He; Cheng Lirui
Source:
Journal of visualized experiments 2022 no.182 pp. e63054
ISSN:
1940-087X
Subject:
quantitative polymerase chain reaction,   etc   ; Phytophthora nicotianae; oats; pathogens; tobacco;     Show all 5 Subjects
Abstract:
... Black shank, caused by the oomycetes Phytophthora nicotianae, is destructive to tobacco, and this pathogen is highly pathogenic to many solanaceous crops. P. nicotianae is well adapted to high temperatures; therefore, research on this pathogen is gaining importance in agriculture worldwide because of global warming. P. nicotianae-resistant varieties of tobacco plants are commonly screened by inocu ...
DOI:
10.3791/63054

7. Survey for Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans in Latvian Water Frogs

Author:
Alena A. Kulikova; Aija Pupina; Mihails Pupins; Andris Čeirāns; Vojtech Baláž
Source:
Journal of wildlife diseases 2022 v.58 no.2 pp. 440-444
ISSN:
0090-3558
Subject:
quantitative polymerase chain reaction,   etc   ; Batrachochytrium dendrobatidis; Pelophylax; surveys; wildlife;     Show all 5 Subjects
Abstract:
... We used quantitative PCR to detect Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bsal) in 135 samples of Pelophylax esculentus complex water frogs from 41 Latvian populations. We detected Bd in 18 populations of water frogs. None of the samples was positive for Bsal. ...
DOI:
10.7589/JWD-D-21-00082

8. Seasonal expression of extracellular signal regulated kinases in the colon of wild ground squirrels (Spermophilus dauricus)

Author:
Yue Song; Xiaoying Yang; Xueying Zhang; Jueyu Zhu; Yixin Chen; Fuli Gao; Haolin Zhang; Yingying Han; Qiang Weng; Zhengrong Yuan
Source:
Molecular biology reports 2022 v.49 no.3 pp. 2209-2215
ISSN:
0301-4851
Subject:
quantitative polymerase chain reaction,   etc   ; Spermophilus; colon; cytoplasm; immunohistochemistry; molecular biology;     Show all 6 Subjects
Abstract:
... BACKGROUND: The purpose of the experiment was to explore the localization and seasonal expression of extracellular signal regulated kinase (ERK) in the colonic tissue of wild ground squirrels (Spermophilus dauricus). METHODS AND RESULTS: Hematoxylin–eosin staining, immunohistochemistry, real-time quantitative PCR and Western blotting were used in this experiment. The histological results showed th ...
DOI:
10.1007/s11033-021-07042-0

9. The role of Wnt pathway antagonists in early-stage lung adenocarcinoma

Author:
Arife Zeybek; Necdet Öz; Serdar Kalemci; Kürşad Tosun; Tuba Gökdoğan Edgünlü; Mehmet Tuğhan Kızıltuğ; Leyla Tekin; Mehmet Emin Erdal
Source:
Molecular biology reports 2022 v.49 no.1 pp. 9-17
ISSN:
0301-4851
Subject:
quantitative polymerase chain reaction,   etc   ; adenocarcinoma; carcinogenesis; lungs; molecular biology; surgery;     Show all 6 Subjects
Abstract:
... PURPOSE: We aimed to examine the expression levels of the genes encoding adenomatous polyposis coli (APC) 1, APC-2, Dickkopf related protein (DKK)-1, DKK-3, secreted frizzled-related protein (SFRP)-2, SFRP-4, and SFRP-5, which play roles in the Wnt signaling pathway, in lung adenocarcinoma and adjacent normal lung tissues and to evaluate their relationships with clinicopathologic factors. MATERIAL ...
DOI:
10.1007/s11033-021-06759-2

10. A New Real-Time PCR Assay for Detecting Fungi in Genus Ceratocystis

Author:
Karthikeyan Dharmaraj; Alice M. Merrall; Julie A. Pattemore; Joanne Mackie; Brett J. R. Alexander; Merje Toome-Heller
Source:
Plant disease 2022 v.106 no.2 pp. 661-668
ISSN:
0191-2917
Subject:
quantitative polymerase chain reaction,   etc   ; Ceratocystis; DNA; biosecurity; figs; genes; plant germplasm;     Show all 7 Subjects
Abstract:
... The genus Ceratocystis contains several significant plant pathogens, causing wilt and canker disease on a wide range of plant species. There are >40 known species of Ceratocystis, some of which are becoming increasingly important in agricultural or natural ecosystems. The diagnostic procedures for most Ceratocystis species rely on time-consuming and labor-intensive culturing approaches. To provide ...
DOI:
10.1094/PDIS-08-21-1639-RE

11. Event-specific PCR methods to quantify the genetically modified DBN9936 maize

Author:
Jun Li; Hongfei Gao; Yunjing Li; Fang Xiao; Shanshan Zhai; Gang Wu; Yuhua Wu
Source:
Journal of food composition and analysis 2022 v.105 pp. 104236
ISSN:
0889-1575
Subject:
quantitative polymerase chain reaction,   etc   ; biosafety; corn; food composition; standard deviation; China;     Show all 6 Subjects
Abstract:
... The genetically modified (GM) maize DBN9936 that has been granted a biosafety certificate needs to be regulated in China. An event-specific PCR assay targeting the junction region between the left border of its T-DNA and the flanking genomic DNA (gDNA) was established to be able to specifically identify the DBN9936 event from other GM maize and non-GM maize. Both qPCR and duplex ddPCR achieved acc ...
DOI:
10.1016/j.jfca.2021.104236

12. Involvement of small-conductance Ca2+-activated K+ (SKCa2) channels in spontaneous Ca2+ oscillations in rat pinealocytes

Author:
Shunsuke Ando; Hiroya Mizutani; Makoto Muramatsu; Yumiko Hagihara; Hiroki Mishima; Rubii Kondo; Yoshiaki Suzuki; Yuji Imaizumi; Hisao Yamamura
Source:
Biochemical and biophysical research communications 2022 pp. -
ISSN:
0006-291X
Subject:
quantitative polymerase chain reaction,   etc   ; acetylcholine; calcium; melatonin; paxilline; rats; research; secretion;     Show all 8 Subjects
Abstract:
... Melatonin secretion from the pineal glands regulates circadian rhythms in mammals. Melatonin production is decreased by an increase in cytosolic Ca²⁺ concentration following the activation of nicotinic acetylcholine receptors in parasympathetic systems. We previously reported that pineal Ca²⁺ oscillations were regulated by voltage-dependent Ca²⁺ channels and large-conductance Ca²⁺-activated K⁺ (BK ...
DOI:
10.1016/j.bbrc.2022.05.052

13. Meta-analysis data of the accuracy of tests for meat adulteration by real-time PCR

Author:
Aisha N. Iskakova; Gulyaim K. Abitayeva; Arman B. Abeev; Zinigul S. Sarmurzina
Source:
Data in Brief 2022 v.41 pp. 107972
ISSN:
2352-3409
Subject:
quantitative polymerase chain reaction,   etc   ; adulterated products; computer software; meat; meta-analysis;     Show all 5 Subjects
Abstract:
... Adulteration of meat products, including illegal substitution and addition of ingredients, tampering, and the misrepresentation and labelling of food or food ingredients, is becoming a more serious problem globally. The consequences of such manipulations can pose various health risks for consumers, including food allergies and poisoning. This study investigates the problem of meat product adultera ...
DOI:
10.1016/j.dib.2022.107972

14. Selection and validation of suitable reference gene for qPCR gene expression analysis in lamb testis cells under Sheep pox virus infection

Author:
Joyshikh Sonowal; Chhabi Lal Patel; Kapil Dev; Rohit Singh; N. Barkathullah; Waseem Akram Malla; Ravi Kumar Gandham; Ravi Kant Agarwal; Deepak Kumar; Shikha Saxena; E. Kalaiselvan; Amitesh Dubey; Krishna Bharali; Raja Ishaq Nabi Khan; BP Mishra; Bina Mishra
Source:
Gene 2022 v.831 pp. 146561
ISSN:
0378-1119
Subject:
quantitative polymerase chain reaction,   etc   ; RNA; Sheeppox virus; gene expression; testes; viruses;     Show all 6 Subjects
Abstract:
... Virus infection alters host gene expression, therefore ideal and stable reference housekeeping genes are required to normalise the expression of other expressed host genes in quantitative real-time PCR (qRT-PCR). The suitable reference gene may vary in response to different viral infections in different hosts or cells. In the present study, we cultured primary lamb testis cells (LTC) and assessed ...
DOI:
10.1016/j.gene.2022.146561

15. Telomeric Repeat-Containing RNA (TERRA): A Review of the Literature and First Assessment in Cutaneous T-Cell Lymphomas

Author:
Alain Chebly; Joana Ropio; Lyla Baldasseroni; Martina Prochazkova-Carlotti; Yamina Idrissi; Jacky Ferrer; Chantal Farra; Marie Beylot-Barry; Jean-Philippe Merlio; Edith Chevret
Source:
Genes 2022 v.13 no.3 pp. -
ISSN:
2073-4425
Subject:
quantitative polymerase chain reaction,   etc   ; DNA; T-cell lymphoma; T-lymphocytes; humans; telomeres;     Show all 6 Subjects
Abstract:
... Telomeric Repeat-containing RNA (TERRA) are long non-coding RNAs transcribed from telomeric DNA sequences from multiple chromosome ends. Major research efforts have been made to understand TERRA roles and functions in several physiological and pathological processes. We summarize herein available data regarding TERRA’s roles in human cells and we report the first investigation in cutaneous T-cells ...
DOI:
10.3390/genes13030539

16. A Long-Amplicon Viability-qPCR Test for Quantifying Living Pathogens that Cause Bacterial Spot in Tomato Seed

Author:
Hehe Wang; Rieanna Wagnon; Daniela Moreno; Sujan Timilsina; Jeffery Jones; Gary Vallad; William W. Turechek
Source:
Plant disease 2022 v.106 no.5 pp. 1474-1485
ISSN:
0191-2917
Subject:
quantitative polymerase chain reaction,   etc   ; DNA; disease-free seeds; inoculum; propidium; seed extracts; tomatoes;     Show all 7 Subjects
Abstract:
... Bacterial spot is one of the most serious diseases of tomato. It is caused by four species of Xanthomonas: X. euvesicatoria, X. gardneri, X. perforans, and X. vesicatoria. Contaminated or infected seed can be a major source of inoculum for this disease. The use of certified pathogen-free seed is one of the primary management practices to reduce the inoculum load in commercial production. Current s ...
DOI:
10.1094/PDIS-11-21-2509-RE

17. Detection by real-time PCR and conventional culture of Salmonella Typhimurium and Listeria monocytogenes adhered to stainless steel surfaces under dry conditions

Author:
Abel Guillermo Ríos-Castillo; Carolina Ripolles-Avila; José Juan Rodríguez-Jerez
Source:
Food control 2022 v.137 pp. 108971
ISSN:
0956-7135
Subject:
quantitative polymerase chain reaction,   etc   ; Listeria monocytogenes; Salmonella Typhimurium; adhesion; food safety; stainless steel;     Show all 6 Subjects
Abstract:
... This study evaluated the capacity of real-time PCR and conventional culture methods to detect Salmonella enterica serovar Typhimurium and Listeria monocytogenes adhered to stainless steel surfaces used as food contact surfaces. The adhesion of the microorganisms to the surfaces was performed under dry conditions to represent the stress to which these pathogens can be subjected in the food processi ...
DOI:
10.1016/j.foodcont.2022.108971

18. Distinct assembly mechanisms of microbial sub-communities with different rarity along the Nu River

Author:
Song Zhang; Kexin Li; Jinming Hu; Fang Wang; Danhong Chen; Zejin Zhang; Ting Li; Linfeng Li; Juan Tao; Dong Liu; Rongxiao Che
Source:
Journal of soils and sediments 2022 v.22 no.5 pp. 1530-1545
ISSN:
1439-0108
Subject:
quantitative polymerase chain reaction,   etc   ; microbial communities; phylogeny; riparian areas; riparian soils; rivers; sediments;     Show all 7 Subjects
Abstract:
... PURPOSE: Microorganisms are an essential component of riverine ecosystems. However, the assembly mechanisms of riverine microbial sub-communities with different rarity are poorly understood. Thus, this study aimed to examine the assembly mechanisms of microbial communities and the sub-communities with different rarity in soils, sediments, and water along the Nu River. MATERIALS AND METHODS: Water, ...
DOI:
10.1007/s11368-022-03149-4

19. Exosome-derived miR-127-5p promotes embryonic-like stem cells differentiation into pacemaker cell through NKx2.5 down-regulation

Author:
Ye Zhu; Jun Tang; Jia You; Xiaojuan Gu; Chao Xu; Xiang Gu
Source:
Biochemical and biophysical research communications 2022 v.611 pp. 68-77
ISSN:
0006-291X
Subject:
quantitative polymerase chain reaction,   etc   ; bioinformatics; cell differentiation; flow cytometry; luciferase; microRNA; prediction; research;     Show all 8 Subjects
Abstract:
... Available evidence suggests the involvement of microRNAs (miRNAs) in the pathological process of several diseases. Nonetheless, molecular mechanism underlying biological effects of miRNAs such as pacemaker exosome-derived miR-127-5p in embryonic-like stem cells (ESCs) differentiation into pacemaker cell is yet to be clarified. Through real time quantitative polymerase chain reaction (qPCR) or west ...
DOI:
10.1016/j.bbrc.2022.04.028

20. Honeybee intestines retain low yeast titers, but no bacterial mutualists, at emergence

Author:
James P. Tauber; Dino McMahon; Eugene V. Ryabov; Magdalena Kunat; Aneta A. Ptaszyńska; Jay D. Evans
Source:
Yeast 2022 v.39 no.1-2 pp. 95-107
ISSN:
0749-503X
Subject:
quantitative polymerase chain reaction,   etc   ; Gilliamella; metamorphosis; microbiome; nutrition; pathogens; pupal development; symbionts; yeasts;     Show all 9 Subjects
Abstract:
... Honeybee symbionts, predominantly bacteria, play important roles in honeybee health, nutrition, and pathogen protection, thereby supporting colony health. On the other hand, fungi are often considered indicators of poor bee health, and honeybee microbiome studies generally exclude fungi and yeasts. We hypothesized that yeasts may be an important aspect of early honeybee biology, and if yeasts prov ...
DOI:
10.1002/yea.3665