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You searched for: Subject "recombinant DNA" Remove constraint Subject: "recombinant DNA"
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1. New strategy for the design, production and pre-purification of chimeric peptide with immunomodulatory activity in Salmosalar

Author:
Carolina Muñoz-Flores; Iván González-Chavarría; Felipe Sandoval; Francisco J. Roa; Paulo Palacios; Allisson Astuya; Khaterina Fernández; Claudia Altamirano; Alex Romero; Jannel Acosta; Jorge R. Toledo
Source:
Fish & shellfish immunology 2022 v.125 pp. 120-127
ISSN:
1050-4648
Subject:
recombinant DNA,   etc   ; Piscirickettsia; Salmo salar; cathelicidins; computer simulation; cytotoxicity; environmental degradation; flagellin; human health; immunomodulators; interleukin-8; kidneys; prediction; salmon; shellfish; synthesis;     Show all 16 Subjects
Abstract:
... The intensive salmon farming is associated with massive outbreaks of infections. The use of antibiotics for their prevention and control is related to damage to the environment and human health. Antimicrobial peptides (AMPs) have been proposed as an alternative to the use of antibiotics for their antimicrobial and immunomodulatory activities. However, one of the main challenges for its massive cli ...
DOI:
10.1016/j.fsi.2022.04.034

2. First monitoring for unauthorized genetically modified bacteria in food enzymes from the food market

Author:
Marie Deckers; Marc De Loose; Nina Papazova; Dieter Deforce; Marie-Alice Fraiture; Nancy H.C. Roosens
Source:
Food control 2022 v.135 pp. 108665
ISSN:
0956-7135
Subject:
recombinant DNA,   etc   ; antibiotic resistance; fermentation; food safety; markets; viability;     Show all 6 Subjects
Abstract:
... Recently, several methods were developed to detect genetically modified (GM) bacteria, allowing to screen for the potential presence of bacteria and to prove bacterial GM contaminations in fermentation products (such as FE). To obtain a first overview of the possible contamination of FE with GM bacteria, a general workflow was proposed, combining recently developed detection methods. Those methods ...
DOI:
10.1016/j.foodcont.2021.108665

3. Insights on the emerging biotechnology of histidine-rich peptides

Author:
Hèctor López-Laguna; Eric Voltà-Durán; Eloi Parladé; Antonio Villaverde; Esther Vázquez; Ugutz Unzueta
Source:
Biotechnology advances 2022 v.54 pp. 107817
ISSN:
0734-9750
Subject:
recombinant DNA,   etc   ; biological production; drugs; histidine; imidazole; peptides; protein synthesis;     Show all 7 Subjects
Abstract:
... In the late 70’s, the discovery of the restriction enzymes made possible the biological production of functional proteins by recombinant DNA technologies, a fact that largely empowered both biotechnological and pharmaceutical industries. Short peptides or small protein domains, with specific molecular affinities, were developed as purification tags in downstream processes to separate the target pr ...
DOI:
10.1016/j.biotechadv.2021.107817

4. Recombitek canine distemper vaccine as an alternative for purevax distemper vaccine in endangered black-footed ferrets (mustela nigripes)

Author:
Mary L. Wright; Travis M. Livieri; Rachel M. Santymire
Source:
Journal of zoo and wildlife medicine 2022 v.53 no.1 pp. 194-199
ISSN:
1937-2825
Subject:
recombinant DNA,   etc   ; Canine morbillivirus; Mustela nigripes; U.S. Fish and Wildlife Service; blood serum; canine distemper; endangered species; ferrets; medicine; vaccination; vaccines; wildlife; zoos; North America;     Show all 14 Subjects
Abstract:
... Black-footed ferrets (Mustela nigripes) are an endangered species in North America that are highly sensitive to canine distemper virus (CDV) infections and any exposure could be devastating to species recovery. The U.S. Fish and Wildlife Service recovery program has safely used a recombinant DNA (rDNA) canarypox-vectored CDV vaccine, Purevax® Ferret Distemper (PFD), to vaccinate black-footed ferre ...
DOI:
10.1638/2020-0228

5. For the sake of the Bioeconomy: define what a Synthetic Biology Chassis is!

Author:
Víctor de Lorenzo; Natalio Krasnogor; Markus Schmidt
Source:
New biotechnology 2021 v.60 pp. 44-51
ISSN:
1871-6784
Subject:
recombinant DNA,   etc   ; Pseudomonas putida; bioeconomics; biotechnology; genomics; soil bacteria; synthetic biology;     Show all 7 Subjects
Abstract:
... At the onset of the 4th Industrial Revolution, the role of synthetic biology (SynBio) as a fuel for the bioeconomy requires clarification of the terms typically adopted by this growing scientific-technical field. The concept of the chassis as a defined, reusable biological frame where non-native components can be plugged in and out to create new functionalities lies at the boundary between frontli ...
DOI:
10.1016/j.nbt.2020.08.004

6. Ovarian superstimulatory response and embryo development using a new recombinant glycoprotein with eCG-like activity in mice

Author:
M. Crispo; M.N. Meikle; G. Schlapp; A. Menchaca
Source:
Theriogenology 2021 v.164 pp. 31-35
ISSN:
0093-691X
Subject:
recombinant DNA,   etc   ; artificial insemination; embryogenesis; glycoproteins; human chorionic gonadotropin; mice; placebos;     Show all 7 Subjects
Abstract:
... Pharmacological ovarian control required for the implementation of artificial insemination and embryo-related technologies usually includes the use of eCG, naturally produced in pregnant mares. In this study, we report the superovulatory response and embryo development in mice obtained with a new glycoprotein with eCG-like activity (reCG) produced by recombinant DNA technology. A total of 150 fema ...
DOI:
10.1016/j.theriogenology.2021.01.012

7. CRISPR ribonucleoprotein-mediated genetic engineering in plants

Author:
Yingxiao Zhang; Brian Iaffaldano; Yiping Qi
Source:
Plant communications 2021 v.2 no.2 pp. 100168
ISSN:
2590-3462
Subject:
recombinant DNA,   etc   ; genomics; mutagenesis; ribonucleoproteins; toxicity;     Show all 5 Subjects
Abstract:
... CRISPR-derived biotechnologies have revolutionized the genetic engineering field and have been widely applied in basic plant research and crop improvement. Commonly used Agrobacterium- or particle bombardment-mediated transformation approaches for the delivery of plasmid-encoded CRISPR reagents can result in the integration of exogenous recombinant DNA and potential off-target mutagenesis. Editing ...
DOI:
10.1016/j.xplc.2021.100168
CHORUS:
10.1016/j.xplc.2021.100168

8. On the way toward regulatable expression systems in acetic acid bacteria: target gene expression and use cases

Author:
Philipp Moritz Fricke; Angelika Klemm; Michael Bott; Tino Polen
Source:
Applied microbiology and biotechnology 2021 v.105 no.9 pp. 3423-3456
ISSN:
0175-7598
Subject:
recombinant DNA,   etc   ; Escherichia coli; Gluconobacter oxydans; acetic acid; biotechnology; databases; gene expression; homoserine; physiology; plasmids;     Show all 10 Subjects
Abstract:
... Acetic acid bacteria (AAB) are valuable biocatalysts for which there is growing interest in understanding their basics including physiology and biochemistry. This is accompanied by growing demands for metabolic engineering of AAB to take advantage of their properties and to improve their biomanufacturing efficiencies. Controlled expression of target genes is key to fundamental and applied microbio ...
DOI:
10.1007/s00253-021-11269-z

9. A novel strategy for efficient expression of an antibody fragment in Escherichia coli: ranibizumab as a case study

Author:
Priyanka Priyanka; Anurag S Rathore
Source:
Journal of chemical technology and biotechnology 2022 v.97 no.1 pp. 42-54
ISSN:
0268-2575
Subject:
recombinant DNA,   etc   ; Escherichia coli; biotechnology; case studies; edema; heterologous gene expression; macular degeneration; mass spectrometry; monoclonal antibodies; polyacrylamide gel electrophoresis; reversed-phase high performance liquid chromatography; ribosomes; surface plasmon resonance; vascular endothelial growth factors;     Show all 14 Subjects
Abstract:
... BACKGROUND: Complex biotherapeutics such as non‐glycosylated antibody fragments can be easily manufactured in Escherichia coli due to advancements in recombinant DNA technology. Ranibizumab is one such example of a complex, non‐glycosylated protein used to treat age‐related macular degeneration and macular oedema. Ranibizumab, a humanized monoclonal antibody fragment, is often expressed as inclusi ...
DOI:
10.1002/jctb.6883

10. In vivo delivery of a multiepitope peptide and Nef protein using novel cell-penetrating peptides for development of HIV-1 vaccine candidate

Author:
Saba Davoodi; Azam Bolhassani; Fatemeh Namazi
Source:
Biotechnology letters 2021 v.43 no.3 pp. 547-559
ISSN:
0141-5492
Subject:
recombinant DNA,   etc   ; adjuvants; agarose; bioinformatics; biotechnology; epitopes; gels; humans; immunoglobulin G; polyacrylamide gel electrophoresis; polypeptides; secretion; vaccines; viruses;     Show all 14 Subjects
Abstract:
... OBJECTIVES: A potent HIV vaccine should overcome some limitations such as polymorphism of human HLA, the diversity of HIV-1 virus, and the lack of an effective delivery system. In this study, a DNA construct encoding Nef₆₀–₈₄, Nef₁₂₆–₁₄₄, Vpr₃₄–₄₇, Vpr₆₀–₇₅, Gp160₃₀–₅₃, Gp160₃₀₈–₃₂₃, and P24₈–₁₅₁ epitopes was designed using bioinformatics tools. The pcDNA3.1-nef-vpr-gp160-p24 and pcDNA3.1-nef cons ...
DOI:
10.1007/s10529-020-03060-3

11. Recent biopharmaceutical applications of capillary electrophoresis methods on recombinant DNA technology‐based products

Author:
S. Irem Kaya; Ahmet Cetinkaya; Mehmet G. Caglayan; Sibel A. Ozkan
Source:
Electrophoresis 2022 v.43 no.9-10 pp. 1035-1049
ISSN:
0173-0835
Subject:
recombinant DNA,   etc   ; amino acid sequences; biopharmaceuticals; blood; capillary electrophoresis; gene therapy; glycosylation; humans; mass spectrometry; molecular weight; peptides;     Show all 11 Subjects
Abstract:
... Biopharmaceuticals (recombinant technology‐based products, vaccines, whole blood and blood components, gene therapy, cells, tissues, etc.,) are described as biological medical products produced from various living sources such as human, microbial, animal, and so on by manufacturing, extraction, or semi‐synthesis. They are complex molecules having high molecular weights. For their safety and effica ...
DOI:
10.1002/elps.202100193

12. Recombinant DNA in fermentation products is of no regulatory relevance

Author:
Alexandra Lensch; Elke Duwenig; Hans-Georg Dederer; Sirpa O. Kärenlampi; René Custers; Agnes Borg; Markus Wyss
Source:
Food control 2022 v.141 pp. 109170
ISSN:
0956-7135
Subject:
recombinant DNA,   etc   ; European Union; biotechnology; breast milk; eggs; equipment; fermentation; food safety; meat; oligosaccharides; safety assessment; vegan diet;     Show all 12 Subjects
Abstract:
... A large variety of fermentation products are used in food and feed production, but also in other industries, and many of these products are produced with genetically modified microorganisms (GMMs). In food and feed production, prominent examples are amino acids, vitamins, food and feed enzymes, colorants, non-caloric sweeteners, human milk oligosaccharides, or vegan alternatives of dairy, egg and ...
DOI:
10.1016/j.foodcont.2022.109170

13. Biotechnology and Biosafety Policy at OECD: Future Trends

Author:
Peter W.E. Kearns; Gijs A. Kleter; Hans E.N. Bergmans; Harry A. Kuiper
Source:
Trends in biotechnology 2021 v.39 no.10 pp. 965-969
ISSN:
0167-7799
Subject:
recombinant DNA,   etc   ; biosafety; biotechnology; issues and policy; safety assessment;     Show all 5 Subjects
Abstract:
... The OECD Council Recommendation on Recombinant DNA Safety Considerations is a legal instrument which has been in force since 1986. It outlines the safety assessment practices that countries should have in place for agricultural and environmental biotechnology. This article suggests possible updates to make it suitable for the modern era. ...
DOI:
10.1016/j.tibtech.2021.03.001

14. Metal and metal(loids) removal efficiency using genetically engineered microbes: Applications and challenges

Author:
Pooja Sharma; Ranjna Sirohi; Yen Wah Tong; Sang Hyoun Kim; Ashok Pandey
Source:
Journal of hazardous materials 2021 v.416 pp. 125855
ISSN:
0304-3894
Subject:
recombinant DNA,   etc   ; RNA; anthropogenic activities; bioaccumulation; economic feasibility; environmental health; food chain; genetic engineering; humans; metabolites; metalloids; pollution; toxicity;     Show all 13 Subjects
Abstract:
... The environment is being polluted in different many with metal and metalloid pollution, mostly due to anthropogenic activity, which is directly affecting human and environmental health. Metals and metalloids are highly toxic at low concentrations and contribute primarily to the survival equilibrium of activities in the environment. However, because of non-degradable, they persist in nature and the ...
DOI:
10.1016/j.jhazmat.2021.125855

15. Engineered Whole-Cell-Based Biosensors: Sensing Environmental Heavy Metal Pollutants in Water—a Review

Author:
Shrute Kannappan; Bhaskar Chandra Mohan Ramisetty
Source:
Applied biochemistry and biotechnology 2022 v.194 no.4 pp. 1814-1840
ISSN:
0273-2289
Subject:
recombinant DNA,   etc   ; bioavailability; biotechnology; brain; drinking water; gene expression; heavy metals; immunosensors; liver; medical treatment; people; toxicity; water quality;     Show all 13 Subjects
Abstract:
... The frequent exposure and accumulation of heavy metals in organisms cause serious health issues affecting a range of organs such as the brain, liver, and reproductive organs in adults, infants, and children. Several parts of the world have high levels of heavy metals affecting millions of people, costing millions of dollars for improving the potability of water and medical treatment of the affecte ...
DOI:
10.1007/s12010-021-03734-2

16. An improved functional analysis of linker-mediated complex (iFALC) strategy

Author:
Yu Nakabayashi; Masahiko Harata; Masayuki Seki
Source:
Biochemical and biophysical research communications 2020 v.526 no.4 pp. 1164-1169
ISSN:
0006-291X
Subject:
recombinant DNA,   etc   ; chromatin; gene targeting; histones; mutation; research; vertebrates; yeasts;     Show all 8 Subjects
Abstract:
... The functional analysis of linker-mediated complex (FALC) strategy that facilitates functional analysis of a common subunit of multi-subunit protein complexes in cells constitutes three steps; (1) a common subunit is fused to a specific subunit via recombinant DNA, (2) mutation is introduced into a portion of the common subunit of the fused protein, and (3) the mutational effect on the fused prote ...
DOI:
10.1016/j.bbrc.2020.04.039

17. Refolding of bioactive human epidermal growth factor from E. coli BL21(DE3) inclusion bodies & evaluations on its in vitro &in vivo bioactivity

Author:
Iman Permana Maksum; Yosua Yosua; Ahmad Nabiel; Riyona Desvy Pratiwi; Sriwidodo Sriwidodo; Ukun M.S. Soedjanaatmadja
Source:
Heliyon 2022 v.8 no.4 pp. e09306
ISSN:
2405-8440
Subject:
recombinant DNA,   etc   ; Escherichia coli; bioactive properties; disulfides; epidermal growth factor; humans; keratinocytes; octoxynol; protein secondary structure; recombinant proteins; solubilization; urea;     Show all 12 Subjects
Abstract:
... Human epidermal growth factor (hEGF) is a mitogenic protein widely used in pharmaceutical and cosmetic industries, thus recombinant DNA technology has been applied to meet the high demand for hEGF. The overexpression of recombinant protein in E. coli often leads to the formation of inclusion bodies (IBs). Mild solubilisation preserves the native secondary protein structure in IBs, thereby the high ...
DOI:
10.1016/j.heliyon.2022.e09306

18. Food adulteration with genetically modified soybeans and maize, meat of animal species and ractopamine residues in different food products

Author:
Amr A. Mostafa; Abd El-Hay G. Abu-Hassiba; Mariam T. ElRouby; Fatma Abou-Hashim; Hanaa S. Omar
Source:
Electronic Journal of Biotechnology 2022 v.55 pp. 65-77
ISSN:
0717-3458
Subject:
recombinant DNA,   etc   ; adulterated foods; asses; biotechnology; chickens; corn; dogs; horses; lard; pork; quantitative polymerase chain reaction; ractopamine; sheep; soybeans; swine; Egypt;     Show all 16 Subjects
Abstract:
... Governments around the world have developed a variety of strategies to address the long-standing food crisis. Food contaminated by genetically modified organisms (GMOs) and meat residues from hormonally treated animals, has recently received increased attention, posing serious health risks to consumers. The aims of this study are to detect recombinant DNA in genetically modified maize, soybeans, a ...
DOI:
10.1016/j.ejbt.2021.11.005

19. The new live imagers MitoMM1/2 for mitochondrial visualization

Author:
Miwa Maeda; Mayu Suzuki; Shigeo Takashima; Tsutomu Sasaki; Kentaro Oh-Hashi; Hiroshi Takemori
Source:
Biochemical and biophysical research communications 2021 v.562 pp. 50-54
ISSN:
0006-291X
Subject:
recombinant DNA,   etc   ; apoptosis; fluorescence; membrane potential; mitochondria; red fluorescent protein; research;     Show all 7 Subjects
Abstract:
... Mitochondria are eukaryotic organelles that consist of outer and inner bilayer membranes with a positive potential (H⁺) in the intermembrane space. This organelle plays an important role in ATP production and apoptosis. To observe the mitochondria in living cells, several fluorescent dyes (such as MitoTracker® [a standard mitochondrial imager] or rhodamine 123) have been developed. However, these ...
DOI:
10.1016/j.bbrc.2021.05.040

20. Engineering of single-domain antibodies for next-generation snakebite antivenoms

Author:
Carla F.C. Fernandes; Soraya S. Pereira; Marcos B. Luiz; Nauanny K.R.L. Silva; Marcela Cristina S. Silva; Anna Carolina M. Marinho; Marcela H.G. Fonseca; Gilvan Pessoa Furtado; Raphael Trevizani; Roberto Nicolete; Andreimar M. Soares; Juliana P. Zuliani; Rodrigo G. Stabeli
Source:
International journal of biological macromolecules 2021 v.185 pp. 240-250
ISSN:
0141-8130
Subject:
recombinant DNA,   etc   ; Camelidae; antivenoms; humans; neutralization; snake bites; snakes; therapeutics; toxicity;     Show all 9 Subjects
Abstract:
... Given the magnitude of the global snakebite crisis, strategies to ensure the quality of antivenom, as well as the availability and sustainability of its supply are under development by several research groups. Recombinant DNA technology has allowed the engineering of monoclonal antibodies and recombinant fragments as alternatives to conventional antivenoms. Besides having higher therapeutic effica ...
DOI:
10.1016/j.ijbiomac.2021.06.043