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1. Tag-Specific Affinity Purification of Recombinant Proteins by Using Molecularly Imprinted Polymers

Author:
Gómez-Arribas, Lidia N.; Urraca, Javier L.; Benito-Peña, Elena; Moreno-Bondi, María C.
Source:
Analytical chemistry 2019 v.91 no.6 pp. 4100-4106
ISSN:
1520-6882
Subject:
recombinant DNA,   etc   ; antibodies; cost effectiveness; epitopes; histidine; peptides; polymers; protein tagging; recombinant proteins; resins;     Show all 10 Subjects
Abstract:
... Epitope tagging is widely used to fuse a known epitope to proteins for which no affinity receptor is available by using recombinant DNA technology. One example is FLAG epitope (DYKDDDDK), which provides better purity and recoveries than the favorite poly histidine tag. However, purification requires using anti-FLAG antibody resins, the high cost and nonreusability of which restrict widespread use. ...
DOI:
10.1021/acs.analchem.8b05731

2. Superactive β-galactosidase inclusion bodies

Author:
Flores, Sandra S.; Nolan, Verónica; Perillo, María A.; Sánchez, Julieta M.
Source:
Colloids and surfaces 2019 v.173 pp. 769-775
ISSN:
0927-7765
Subject:
recombinant DNA,   etc   ; beta-galactosidase; colloids; enzyme kinetics; enzyme stability; inclusion bodies; lactose; pH; protein synthesis; temperature; washing;     Show all 11 Subjects
Abstract:
... Bacterial inclusion bodies (IBs) were historically considered one of the major obstacles in protein production through recombinant DNA techniques and conceived as amorphous deposits formed by passive and rather unspecific structures of unfolded proteins aggregates. Subsequent studies demonstrated that IBs contained an important quantity of active protein. In this work, we proved that recombinant β ...
DOI:
10.1016/j.colsurfb.2018.10.049

3. Structural studies on dihydrouridine synthase A (DusA) from Pseudomonas aeruginosa

Author:
Goyal, Nainee; Chandra, Anshuman; Qamar, Imteyaz; Singh, Nagendra
Source:
International journal of biological macromolecules 2019 v.132 pp. 254-264
ISSN:
0141-8130
Subject:
recombinant DNA,   etc   ; Pseudomonas aeruginosa; catalytic activity; circular dichroism spectroscopy; drug design; enzymes; genes; monitoring; puromycin; temperature; transfer RNA; translation (genetics); urea; uridine;     Show all 14 Subjects
Abstract:
... Dihydrouridination is one of the abundant modifications in tRNA editing. The presence of dihydrouridine is attributed to tRNA stability desired for the efficient gene translation process. The conversion of uridine to dihydrouridine is catalyzed by flavine containing enzyme called dihydrouridine synthase (Dus). We report first ever information about DusA enzyme from Pseudomonas aeruginosa in form o ...
DOI:
10.1016/j.ijbiomac.2019.03.209

4. Evaluation of the expression stability of β-actin under bacterial infection in Macrobrachium nipponense

Author:
Geng, Wen-Yi; Yao, Feng-Jiao; Tang, Ting; Shi, Shan-Shan
Source:
Molecular biology reports 2019 v.46 no.1 pp. 309-315
ISSN:
0301-4851
Subject:
recombinant DNA,   etc   ; Aeromonas hydrophila; Escherichia coli; Macrobrachium nipponense; actin; bacteria; bacterial infections; bioinformatics; databases; gene expression; genes; genetic vectors; nickel; phylogeny; quantitative polymerase chain reaction; shrimp;     Show all 16 Subjects
Abstract:
... The selection of a suitable reference gene is an important prerequisite for the precise analysis of target gene expression by real-time quantitative PCR (qPCR). The present study aims to explore the expression pattern of the Macrobrachium nipponense (M. nipponense) β-actin gene under Aeromonas hydrophila bacterial infection conditions. The complete sequence of the β-actin gene from M. nipponense w ...
DOI:
10.1007/s11033-018-4473-4

5. Application of recombinant hemagglutinin proteins as alternative antigen standards for pandemic influenza vaccines

Author:
Choi, Yejin; Kwon, Seong Yi; Oh, Ho Jung; Shim, Sunbo; Chang, Seokkee; Chung, Hye Joo; Kim, Do Keun; Park, Younsang; Lee, Younghee
Source:
Biotechnology letters 2017 v.39 no.9 pp. 1375-1380
ISSN:
0141-5492
Subject:
recombinant DNA,   etc   ; antigens; hemagglutinins; influenza vaccines; pandemic;     Show all 5 Subjects
Abstract:
... OBJECTIVES: The single radial immunodiffusion (SRID) assay, used to quantify hemagglutinin (HA) in influenza vaccines, requires reference reagents; however, because centralized production of reference reagents may slow the emergency deployment of vaccines, alternatives are needed. RESULTS: We investigated the production of HA proteins using recombinant DNA technology, rather than a traditional egg ...
DOI:
10.1007/s10529-017-2372-8

6. A Dengue Vaccine

Author:
Durbin, Anna P.
Source:
Cell 2016 v.166 pp. 1
ISSN:
0092-8674
Subject:
recombinant DNA,   etc   ; dengue; live vaccines;     Show all 3 Subjects
Abstract:
... Denvaxia is the first licensed vaccine for the prevention of dengue. It is a live vaccine developed using recombinant DNA technology. The vaccine is given as three doses over the course of a year and has the potential to prevent hundreds of thousands of hospitalizations each year. ...
DOI:
10.1016/j.cell.2016.06.036

7. Pyrite cloning: a single tube and programmed reaction cloning with restriction enzymes

Author:
Fischer, Matthew D.; Mgboji, Emmanuel; Liu, Zhongchi
Source:
Plant methods 2018 v.14 no.1 pp. 91
ISSN:
1746-4811
Subject:
recombinant DNA,   etc   ; DNA fragmentation; polymerase chain reaction; pyrite; restriction endonucleases; screening;     Show all 6 Subjects
Abstract:
... BACKGROUND: Insertion of engineered DNA fragments into bacterial vectors is the foundation of recombinant DNA technology, yet existing methods are still laborious, require many steps, depend on specific vector configuration, or require expensive reagents. RESULTS: We have developed a method, called “Pyrite” cloning that combines the traditional restriction enzyme digestion and ligation reaction in ...
DOI:
10.1186/s13007-018-0359-7

8. Comparison of footbathing and vaccination to control ovine footrot in an experimentally infected flock

Author:
Allworth, MB; Egerton, JR
Source:
Australian veterinary journal 2018 v.96 no.10 pp. 395-399
ISSN:
0005-0423
Subject:
recombinant DNA,   etc   ; Dichelobacter nodosus; flocks; footrot; pastures; recombinant vaccines; sheep; vaccination; virulent strains; zinc sulfate;     Show all 10 Subjects
Abstract:
... OBJECTIVE: Compare footbathing and vaccination for control of footrot during a transmission period in a sheep flock deliberately infected with multiple strains of Dichelobacter nodosus. METHODS: The strains included a known virulent strain, a benign strain and several intermediate strains. The resulting footrot was clinically intermediate. A total of 1450 Polwarth sheep aged 1–3 years were allocat ...
DOI:
10.1111/avj.12715

9. High-throughput, one-step screening, cloning and expression based on the lethality of DpnI in Escherichia coli

Author:
Wu, Peijie; Li, Xiaoyan; Yang, Maocheng; Huang, Zhengzhi; Mo, Hongya; Li, Ting; Zhang, Yuan; Li, Hongtao
Source:
Biochemical and biophysical research communications 2018 v.504 no.1 pp. 177-183
ISSN:
0006-291X
Subject:
recombinant DNA,   etc   ; DNA libraries; Escherichia coli; death; enzymes; genetic vectors; molecular biology; proteins; screening; therapeutics; toxicity;     Show all 11 Subjects
Abstract:
... The manipulation of recombinant DNA has been an integral step in molecular biology to date. A number of strategies have been developed over the years, as traditional cloning methods are time consuming, have high backgrounds and low efficiency and are often limited by the number of suitable restriction sites available. Here, we constructed a series of new positive-selection-based cloning vectors th ...
DOI:
10.1016/j.bbrc.2018.08.151

10. Synthetic biology for microbial heavy metal biosensors

Author:
Kim, HyunJu; Jeong, Haeyoung; Lee, SangJun
Source:
Analytical and bioanalytical chemistry 2018 v.410 no.4 pp. 1191-1203
ISSN:
1618-2642
Subject:
recombinant DNA,   etc   ; artificial cells; biosensors; databases; genes; heavy metals; metal ions; pollutants; sequence analysis; synthetic biology;     Show all 10 Subjects
Abstract:
... Using recombinant DNA technology, various whole-cell biosensors have been developed for detection of environmental pollutants, including heavy metal ions. Whole-cell biosensors have several advantages: easy and inexpensive cultivation, multiple assays, and no requirement of any special techniques for analysis. In the era of synthetic biology, cutting-edge DNA sequencing and gene synthesis technolo ...
DOI:
10.1007/s00216-017-0751-6

11. A self-assembled DNA-nanoparticle with a targeting peptide for hypoxia-inducible gene therapy of ischemic stroke

Author:
Oh, Jungju; Lee, Jaewon; Piao, Chunxian; Jeong, Ji Hoon; Lee, Minhyung
Source:
Biomaterials science 2019 v.7 no.5 pp. 2174-2190
ISSN:
2047-4849
Subject:
recombinant DNA,   etc   ; animal models; apoptosis; brain; gene expression; gene therapy; genes; heme oxygenase (biliverdin-producing); hypoxia-inducible factor 1; inducible nitric oxide synthase; infarction; inflammation; ligands; nanoparticles; peptides; plasmids; reactive oxygen species; signal transduction; stroke; tissues; transfection;     Show all 21 Subjects
Abstract:
... A self-assembled nanoparticle composed of hypoxia-specific anti-RAGE peptide (HSAP), heme oxygenase-1 plasmid (pHO1), and deoxycholate-conjugated polyethylenimine-2k (DP2k) was developed for ischemic stroke therapy. RAGE is over-expressed and induces inflammation in the ischemic brain. To inhibit RAGE-mediated signal transduction, HSAP was produced by recombinant DNA technology, based on the RAGE- ...
DOI:
10.1039/c8bm01621f

12. Production of protein-based polymers in Pichia pastoris

Author:
Werten, Marc W.T.; Eggink, Gerrit; Cohen Stuart, Martien A.; de Wolf, Frits A.
Source:
Biotechnology advances 2019
ISSN:
0734-9750
Subject:
recombinant DNA,   etc   ; Escherichia coli; Pichia pastoris; amino acid sequences; biomedical research; bioprocessing; bioreactors; biosynthesis; collagen; elastin; genetic engineering; liquid state fermentation; molecular weight; nanomaterials; physical properties; polymers; process control; protein synthesis; proteolysis; silk; yeasts;     Show all 21 Subjects
Abstract:
... Materials science and genetic engineering have joined forces over the last three decades in the development of so-called protein-based polymers. These are proteins, typically with repetitive amino acid sequences, that have such physical properties that they can be used as functional materials. Well-known natural examples are collagen, silk, and elastin, but also artificial sequences have been devi ...
DOI:
10.1016/j.biotechadv.2019.03.012

13. Roscovitine and Trichostatin A promote DNA damage repair during porcine oocyte maturation

Author:
Zhang, Bingyue; Niu, Huiran; Cai, Qingqing; Liao, Mengqin; Chen, Keren; Chen, Yaosheng; Cong, Peiqing
Source:
Reproduction, fertility, and development 2019 v.31 no.3 pp. 473-481
ISSN:
1031-3613
Subject:
recombinant DNA,   etc   ; DNA damage; DNA repair; X-radiation; blastocyst; breast neoplasms; embryogenesis; etoposide; extrusion; fluorescent antibody technique; genes; germinal vesicle; meiosis; messenger RNA; protein kinases; protein subunits; swine; tumor suppressor proteins;     Show all 18 Subjects
Abstract:
... Faithful repair of DNA double-strand breaks in mammalian oocytes is essential for meiotic maturation and embryonic development. In the present study we investigated the roles of Roscovitine and Trichostatin A (TSA) in DNA damage recovery during invitro maturation of porcine oocytes. Etoposide was used to trigger DNA damage in oocytes. When these DNA-damaged oocytes were treated with 2μM Roscovitin ...
DOI:
10.1071/RD18021

14. Production of bioactive hepcidin by recombinant DNA tagging with an elastin-like recombinamer

Author:
da Costa, A.; Pereira, A.M.; Gomes, A.C.; Rodriguez-Cabello, J C; Casal, M.; Machado, R.
Source:
New biotechnology 2018 v.46 pp. 45-53
ISSN:
1871-6784
Subject:
recombinant DNA,   etc   ; Escherichia coli; antibacterial properties; antibiotics; antiviral properties; chromatography; hepcidin; humans; pathogens; recombinant proteins; thermal properties;     Show all 11 Subjects
Abstract:
... With the lack of new chemical antibiotics and increasing pathogen resistance to those available, new alternatives are being explored. Antimicrobial peptides (AMPs) with a broad range of effects, including antibacterial, antifungal, and antiviral actions, have emerged as one of the options. They can be produced by recombinant DNA technology, but the chromatographic methods used for peptide purifica ...
DOI:
10.1016/j.nbt.2018.07.001

15. MARSI: metabolite analogues for rational strain improvement

Author:
Cardoso, João G R; Zeidan, Ahmad A; Jensen, Kristian; Sonnenschein, Nikolaus; Neves, Ana Rute; Herrgård, Markus J
Source:
Bioinformatics 2018 v.34 no.13 pp. 2319-2321
ISSN:
1460-2059
Subject:
recombinant DNA,   etc   ; bioinformatics; chemical reactions; culture media; databases; drugs; enzymatic reactions; genes; metabolites; microorganisms; models; mutants; phenotype;     Show all 13 Subjects
Abstract:
... Metabolite analogues (MAs) mimic the structure of native metabolites, can competitively inhibit their utilization in enzymatic reactions, and are commonly used as selection tools for isolating desirable mutants of industrial microorganisms. Genome-scale metabolic models representing all biochemical reactions in an organism can be used to predict effects of MAs on cellular phenotypes. Here, we pres ...
DOI:
10.1093/bioinformatics/bty108

16. Synthesis and Inhibitory Properties of Imines Containing Monoterpenoid and Adamantane Fragments Against DNA Repair Enzyme Tyrosyl-DNA Phosphodiesterase 1 (Tdp1)

Author:
Zakharenko, A. L.; Mozhaitsev, E. S.; Suslov, E. V.; Korchagina, D. V.; Volcho, K. P.; Salakhutdinov, N. F.; Lavrik, O. I.
Source:
Chemistry of natural compounds 2018 v.54 no.4 pp. 672-676
ISSN:
0009-3130
Subject:
recombinant DNA,   etc   ; DNA repair; aldehydes; chemistry; enzyme inhibition; enzyme inhibitors; humans; imines; inhibitory concentration 50; monoterpenoids; phosphodiesterase I;     Show all 11 Subjects
Abstract:
... Six imines including four new ones were synthesized via the reaction of monoterpenoid aldehydes with aminoadamantanes. The inhibitory activities of the synthesized compounds against purified human recombinant DNA repair enzyme tyrosyl-DNA phosphodiesterase 1 (Tdp1) fell in the range IC₅₀ = 5.5–7.1 μM. ...
DOI:
10.1007/s10600-018-2443-7

17. Capsule-like Safe Genetic Vectors—Cell-Penetrating Core–Shell Particles Selectively Release Functional Small RNA and Entrap Its Encoding DNA

Author:
Yu, Han; Pan, Houwen Matthew; Evalin,; Trau, Dieter; Patzel, Volker
Source:
ACS applied materials & interfaces 2018 v.10 no.25 pp. 21113-21124
ISSN:
1944-8252
Subject:
recombinant DNA,   etc   ; cell viability; encapsulation; genetic vectors; genome; humans; monocytes; particle size; small interfering RNA; tissue culture; vaccination;     Show all 11 Subjects
Abstract:
... The breakthrough of genetic therapy is set back by the lack of suitable genetic vector systems. We present the development of permeability-tunable, capsule-like, polymeric, micron-sized, core–shell particles for delivery of recombinant nucleic acids into target cells. These particles were demonstrated to effectively release rod-shaped small hairpin RNA and to selectively retain the RNA-encoding DN ...
DOI:
10.1021/acsami.8b04294

18. Hinge-Type Dimerization of Proteins by a Tetracysteine Peptide of High Pairing Specificity

Author:
Schrimpf, Andreas; Hempel, Franziska; Li, Aitao; Linne, Uwe; Maier, Uwe G.; Reetz, Manfred T.; Geyer, Armin
Source:
Biochemistry 2018 v.57 no.26 pp. 3658-3664
ISSN:
1520-4995
Subject:
recombinant DNA,   etc   ; Bacillariophyceae; dimerization; epoxide hydrolase; limonene; mass spectrometry; mixing; monoclonal antibodies; nuclear magnetic resonance spectroscopy; peptides; proteins; regioselectivity; thiols;     Show all 13 Subjects
Abstract:
... Dimeric disulfide-linked peptides are formed by the regioselective oxidative folding of thiol precursors containing the CX₃CX₂CX₃C tetracysteine motif. Here, we investigate the general applicability of this peptide as a dimerization motif for different proteins. By recombinant DNA technology, the peptide CHWECRGCRLVC was loaded with proteins, and functional homodimers were obtained upon oxidative ...
DOI:
10.1021/acs.biochem.8b00475

19. Collapse of DNA in packaging and cellular transport

Author:
Thomas, T.J.; Thomas, Thresia
Source:
International journal of biological macromolecules 2018 v.109 pp. 36-48
ISSN:
0141-8130
Subject:
recombinant DNA,   etc   ; DNA packaging; cations; cell membranes; dendrimers; gene therapy; molecular biology; nanoparticles; oligopeptides; packaging; polypeptides; spermidine; spermine;     Show all 13 Subjects
Abstract:
... The dawn of molecular biology and recombinant DNA technology arose from our ability to manipulate DNA, including the process of collapse of long extended DNA molecules into nanoparticles of approximately 100 nm diameter. This condensation process is important for the packaging of DNA in the cell and for transporting DNA through the cell membrane for gene therapy. Multivalent cations, such as natur ...
DOI:
10.1016/j.ijbiomac.2017.12.076

20. Barcoded sequencing workflow for high throughput digitization of hybridoma antibody variable domain sequences

Author:
Chen, Yongmei; Kim, Si Hyun; Shang, Yonglei; Guillory, Joseph; Stinson, Jeremy; Zhang, Qing; Hötzel, Isidro; Hoi, Kam Hon
Source:
Journal of immunological methods 2018 v.455 pp. 88-94
ISSN:
0022-1759
Subject:
recombinant DNA,   etc   ; B-lymphocytes; antigens; bioinformatics; clones; complement; cryopreservation; high-throughput nucleotide sequencing; hybridomas; monoclonal antibodies; robots; screening; storage;     Show all 13 Subjects
Abstract:
... Since the invention of Hybridoma technology by Milstein and Köhler in 1975, its application has greatly advanced the antibody discovery process. The technology enables both functional screening and long-term archival of the immortalized monoclonal antibody producing B cells. Despite the dependable cryopreservation technology for hybridoma cells, practicality of long-term storage has been outpaced ...
DOI:
10.1016/j.jim.2018.01.004