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1. CRISPR ribonucleoprotein-mediated genetic engineering in plants

Author:
Zhang, Yingxiao; Iaffaldano, Brian; Qi, Yiping
Source:
Plant communications 2021 v.2 no.2 pp. 100168
ISSN:
2590-3462
Subject:
recombinant DNA,   etc   ; genomics; mutagenesis; ribonucleoproteins; toxicity;     Show all 5 Subjects
Abstract:
... CRISPR-derived biotechnologies have revolutionized the genetic engineering field and have been widely applied in basic plant research and crop improvement. Commonly used Agrobacterium- or particle bombardment-mediated transformation approaches for the delivery of plasmid-encoded CRISPR reagents can result in the integration of exogenous recombinant DNA and potential off-target mutagenesis. Editing ...
DOI:
10.1016/j.xplc.2021.100168

2. The new live imagers MitoMM1/2 for mitochondrial visualization

Author:
Maeda, Miwa; Suzuki, Mayu; Takashima, Shigeo; Sasaki, Tsutomu; Oh-Hashi, Kentaro; Takemori, Hiroshi
Source:
Biochemical and biophysical research communications 2021 v.562 pp. 50-54
ISSN:
0006-291X
Subject:
recombinant DNA,   etc   ; apoptosis; fluorescence; membrane potential; mitochondria; red fluorescent protein; research;     Show all 7 Subjects
Abstract:
... Mitochondria are eukaryotic organelles that consist of outer and inner bilayer membranes with a positive potential (H⁺) in the intermembrane space. This organelle plays an important role in ATP production and apoptosis. To observe the mitochondria in living cells, several fluorescent dyes (such as MitoTracker® [a standard mitochondrial imager] or rhodamine 123) have been developed. However, these ...
DOI:
10.1016/j.bbrc.2021.05.040

3. Engineering of single-domain antibodies for next generation snakebite antivenoms

Author:
Fernandes, Carla F.C.; Pereira, Soraya S.; Luiz, Marcos B.; Silva, Nauanny K.R.L.; da Silva, Marcela Cristina; Marinho, Anna Carolina M.; Fonseca, Marcela H.G.; Furtado, Gilvan Pessoa; Trevizani, Raphael; Nicolete, Roberto; Soares, Andreimar M.; Zuliani, Juliana P.; Stabeli, Rodrigo G.
Source:
International journal of biological macromolecules 2021
ISSN:
0141-8130
Subject:
recombinant DNA,   etc   ; Camelidae; antivenoms; humans; neutralization; snake bites; snakes; therapeutics; toxicity;     Show all 9 Subjects
Abstract:
... Given the magnitude of the global snakebite crisis, strategies to ensure the quality of antivenom, as well as the availability and sustainability of its supply are under development by several research groups. Recombinant DNA technology has allowed the engineering of monoclonal antibodies and recombinant fragments as alternatives to conventional antivenoms. Besides higher therapeutic efficacy, wit ...
DOI:
10.1016/j.ijbiomac.2021.06.043

4. Ovarian superstimulatory response and embryo development using a new recombinant glycoprotein with eCG-like activity in mice

Author:
Crispo, M.; Meikle, M.N.; Schlapp, G.; Menchaca, A.
Source:
Theriogenology 2021 v.164 pp. 31-35
ISSN:
0093-691X
Subject:
recombinant DNA,   etc   ; artificial insemination; embryogenesis; glycoproteins; human chorionic gonadotropin; mice; placebos;     Show all 7 Subjects
Abstract:
... Pharmacological ovarian control required for the implementation of artificial insemination and embryo-related technologies usually includes the use of eCG, naturally produced in pregnant mares. In this study, we report the superovulatory response and embryo development in mice obtained with a new glycoprotein with eCG-like activity (reCG) produced by recombinant DNA technology. A total of 150 fema ...
DOI:
10.1016/j.theriogenology.2021.01.012

5. Metal and metal(loids) removal efficiency using genetically engineered microbes: Applications and challenges

Author:
Sharma, Pooja; Sirohi, Ranjna; Tong, Yen Wah; Kim, Sang Hyoun; Pandey, Ashok
Source:
Journal of hazardous materials 2021 v.416 pp. 125855
ISSN:
0304-3894
Subject:
recombinant DNA,   etc   ; RNA; anthropogenic activities; bioaccumulation; economic feasibility; environmental health; food chain; genetic engineering; humans; metabolites; metalloids; pollution; toxicity;     Show all 13 Subjects
Abstract:
... The environment is being polluted in different many with metal and metalloid pollution, mostly due to anthropogenic activity, which is directly affecting human and environmental health. Metals and metalloids are highly toxic at low concentrations and contribute primarily to the survival equilibrium of activities in the environment. However, because of non-degradable, they persist in nature and the ...
DOI:
10.1016/j.jhazmat.2021.125855

6. Rapid detection of H146-like goose calicivirus using a TaqMan-based real-time PCR assay

Author:
Zheng, Min; Lin, Su; Zhang, Shizhong; Chen, Xiuqin; Jiang, Dandan; Chen, Shaoying; Wang, Shao; Chen, Shilong
Source:
Poultry science 2021 v.100 no.2 pp. 482-487
ISSN:
0032-5791
Subject:
recombinant DNA,   etc   ; Goose calicivirus; Vesivirus; cross reaction; epidemiology; genes; plasmids; quantitative polymerase chain reaction; rapid methods; viruses; waterfowl;     Show all 11 Subjects
Abstract:
... H146-like goose-origin calicivirus (H146-like GCV) is a novel Caliciviridae family member in the Sanovirus genus that was recently discovered and proposed to cause runting-stunting syndrome and urate deposition in geese. At present, however, there is a lack of epidemiological information pertaining to the dynamics and distribution of H146-like GCV. The development of novel molecular diagnostic app ...
DOI:
10.1016/j.psj.2020.11.016

7. Development of a Novel Detection Method Targeting an Ultrashort 25 bp Sequence Found in Agrobacterium-Mediated Transformed GM Plants

Author:
Takabatake, Reona; Onishi, Mari; Minegishi, Yasutaka; Futo, Satoshi; Soga, Keisuke; Nakamura, Kosuke; Kondo, Kazunari; Mano, Junichi; Kitta, Kazumi
Source:
Journal of agricultural and food chemistry 2020 v.68 no.51 pp. 15327-15334
ISSN:
1520-5118
Subject:
recombinant DNA,   etc   ; chromatography; food chemistry; genome; oligodeoxyribonucleotides; polymerase chain reaction;     Show all 6 Subjects
Abstract:
... Agrobacterium-mediated transformation is the most commonly used technique for plant genetic engineering. During the transformation, a T-DNA region, which is flanked by the right border (RB) and the left border, is transferred to plant nuclear chromosomes. Simultaneously, a sequence adjacent to the RB on T-DNA is frequently transferred to plant genomes together with the intentionally introduced rec ...
DOI:
10.1021/acs.jafc.0c03864

8. An improved functional analysis of linker-mediated complex (iFALC) strategy

Author:
Nakabayashi, Yu; Harata, Masahiko; Seki, Masayuki
Source:
Biochemical and biophysical research communications 2020 v.526 no.4 pp. 1164-1169
ISSN:
0006-291X
Subject:
recombinant DNA,   etc   ; chromatin; gene targeting; histones; mutation; research; vertebrates; yeasts;     Show all 8 Subjects
Abstract:
... The functional analysis of linker-mediated complex (FALC) strategy that facilitates functional analysis of a common subunit of multi-subunit protein complexes in cells constitutes three steps; (1) a common subunit is fused to a specific subunit via recombinant DNA, (2) mutation is introduced into a portion of the common subunit of the fused protein, and (3) the mutational effect on the fused prote ...
DOI:
10.1016/j.bbrc.2020.04.039

9. DiRect: Site-directed mutagenesis method for protein engineering by rational design

Author:
Watanabe, Satoru; Ito, Masahiro; Kigawa, Takanori
Source:
Biochemical and biophysical research communications 2021 v.551 pp. 107-113
ISSN:
0006-291X
Subject:
recombinant DNA,   etc   ; NAD (coenzyme); Rhodotorula rubra; cell-free protein synthesis; molecular biology; mutants; oxidoreductases; polymerase chain reaction; product quality; research; site-directed mutagenesis; thermal stability;     Show all 12 Subjects
Abstract:
... Site-directed mutagenesis (SDM), an indispensable method in molecular biology and protein engineering, is rather time-consuming and laborious. Protein engineering, especially that of enzymes, nowadays increasingly relies on rational design approaches in which both SDM and protein expression are the bottlenecks because they are generally based on the recombinant DNA technology. Here, we developed a ...
DOI:
10.1016/j.bbrc.2021.03.021

10. Molecular Biology in the Improvement of Biological Nitrogen Fixation by Rhizobia and Extending the Scope to Cereals

Author:
Goyal, Ravinder K.; Schmidt, Maria Augusta; Hynes, Michael F.
Source:
Microorganisms 2021 v.9 no.1
ISSN:
2076-2607
Subject:
recombinant DNA,   etc   ; bacteria; crop production; grains; knowledge; molecular biology; nitrogen; nitrogen fertilizers; nitrogen fixation; production technology; staple crops; strains; symbiosis; total nitrogen;     Show all 14 Subjects
Abstract:
... The contribution of biological nitrogen fixation to the total N requirement of food and feed crops diminished in importance with the advent of synthetic N fertilizers, which fueled the “green revolution”. Despite being environmentally unfriendly, the synthetic versions gained prominence primarily due to their low cost, and the fact that most important staple crops never evolved symbiotic associati ...
DOI:
10.3390/microorganisms9010125

11. Quantification of knowledge content of a high impact innovation: recombinant DNA

Author:
Tseng, Hung; Small, Henry
Source:
Heliyon 2019 v.5 no.8 pp. e02219
ISSN:
2405-8440
Subject:
recombinant DNA,   etc   ; paper; quantitative analysis; technology;     Show all 4 Subjects
Abstract:
... Quantitative analysis of knowledge content of a significant technological innovation is a novel approach to understand the scientific discovery process. Here we describe such an analysis applied to the invention of recombinant DNA technology in the early 1970's. Two focal papers are selected, i.e., Jackson et al., 1972 and Cohen et al., 1973. A knowledge framework called EApc is described to categ ...
DOI:
10.1016/j.heliyon.2019.e02219
PubMed:
31517081
PubMed Central:
PMC6728730

12. Timeline: Vaccines

Author:
Rappuoli, Rino
Source:
Cell 2020 v.183 no.2 pp. 552
ISSN:
0092-8674
Subject:
recombinant DNA,   etc   ; COVID-19 infection; cell culture; cows; genomics; synthetic biology; travel; vaccination; viral growth; viruses;     Show all 10 Subjects
Abstract:
... Infectious diseases killed hundreds of million people, made empires and civilizations vulnerable, and recently took away our freedom to go out, walk, work, travel, meet friends, and visit relatives during the Covid-19 lockdown. Vaccination freed humans from most of infectious diseases of the past. By starting with a virus grown in a cow, vaccination has been propelled by new technologies such as v ...
DOI:
10.1016/j.cell.2020.09.039

13. Studies on DNA-related enzymes to elucidate molecular mechanisms underlying genetic information processing and their application in genetic engineering

Author:
Ishino, Yoshizumi
Source:
Bioscience, biotechnology, and biochemistry 2020 v.84 no.9 pp. 1749-1766
ISSN:
1347-6947
Subject:
recombinant DNA,   etc   ; CRISPR-Cas systems; biotechnology; gene editing; molecular biology; polymerase chain reaction;     Show all 6 Subjects
Abstract:
... Recombinant DNA technology, in which artificially “cut and pasted” DNA in vitro is introduced into living cells, contributed extensively to the rapid development of molecular biology over the past 5 decades since the latter half of the 20ᵗʰ century. Although the original technology required special experiences and skills, the development of polymerase chain reaction (PCR) has greatly eased in vitr ...
DOI:
10.1080/09168451.2020.1778441

14. Optimization of a novel visible-light-driven Ag/C-TiO2 nanophotocatalyst for treatment of recombinant DNA in biopharmaceutical wastewater

Author:
Mahmoudian-Boroujerd, L.; Hosseini, S. N.; Karimi-Jashni, A.; Khatami, M.; Javadpour, S.
Source:
International journal of environmental science and technology 2021 v.18 no.4 pp. 885-900
ISSN:
1735-1472
Subject:
recombinant DNA,   etc   ; Fourier transform infrared spectroscopy; X-ray diffraction; biopharmaceuticals; carbon; energy-dispersive X-ray analysis; hepatitis B antigens; pH; reflectance; response surface methodology; silver; statistical analysis; temperature; wastewater;     Show all 14 Subjects
Abstract:
... The aim of this research was to optimize and model the synthesis and application of a novel visible-light-driven Ag/C-TiO₂ nanophotocatalyst for degradation of recombinant DNA in Hepatitis B surface antigen production plant wastewater. The synthesized nanoparticles were characterized using X-ray diffraction, Fourier transform infrared spectroscopy, UV–Vis diffuse reflectance spectra, field emissio ...
DOI:
10.1007/s13762-020-02881-z

15. Transgenic models for investigating the nervous system: Currently available neurofluorescent reporters and potential neuronal markers

Author:
Yamakawa, Michael; Santosa, Samuel M.; Chawla, Neeraj; Ivakhnitskaia, Evguenia; del Pino, Matthew; Giakas, Sebastian; Nadel, Arnold; Bontu, Sneha; Tambe, Arjun; Guo, Kai; Han, Kyu-Yeon; Cortina, Maria Soledad; Yu, Charles; Rosenblatt, Mark I.; Chang, Jin-Hong; Azar, Dimitri T.
Source:
Biochimica et biophysica acta 2020 v.1864 no.7 pp. 129595
ISSN:
0304-4165
Subject:
recombinant DNA,   etc   ; adults; animal models; fluorescence; nerve tissue; neurogenesis; neurons; peripheral nervous system; promoter regions; proteins; therapeutics; transgenic animals;     Show all 12 Subjects
Abstract:
... Recombinant DNA technologies have enabled the development of transgenic animal models for use in studying a myriad of diseases and biological states. By placing fluorescent reporters under the direct regulation of the promoter region of specific marker proteins, these models can localize and characterize very specific cell types. One important application of transgenic species is the study of the ...
DOI:
10.1016/j.bbagen.2020.129595
PubMed:
32173376
PubMed Central:
PMC7196036

16. Biological treatment of plant biomass and factors affecting bioactivity

Author:
Singh, Sandip K.
Source:
Journal of cleaner production 2021 v.279 pp. 123546
ISSN:
0959-6526
Subject:
recombinant DNA,   etc   ; bacteria; bioactive properties; biofuels; biological treatment; biorefining; catalytic activity; cellulose; delignification; endo-1,4-beta-glucanase; energy; enzymatic hydrolysis; enzymatic treatment; enzyme activity; feedstocks; fungi; greenhouse gas emissions; hemicellulose; hydrolysis; hydroxyl radicals; laccase; lignin; lignin peroxidase; manganese peroxidase; peroxidase; phytomass; polygalacturonase; sugars;     Show all 28 Subjects
Abstract:
... Plant biomass is a most promising renewable, sustainable and imminent candidate to mitigate the greenhouse gas emission effect, and can be applied in various ways to produce biofuels, chemicals, materials and polymers through integrated biorefineries processes. Pretreatment is an initial step of plant biorefineries and plays a crucial role to separate cellulose, hemicellulose and lignin. Pretreatm ...
DOI:
10.1016/j.jclepro.2020.123546

17. Are antimicrobial resistance genes key targets to detect genetically modified microorganisms in fermentation products?

Author:
Fraiture, Marie-Alice; Deckers, Marie; Papazova, Nina; Roosens, Nancy H.C.
Source:
International journal of food microbiology 2020 v.331 pp. 108749
ISSN:
0168-1605
Subject:
recombinant DNA,   etc   ; antibiotic resistance; antibiotic resistance genes; bacteria; chloramphenicol; feed industry; feeds; fermented foods; flavorings; kanamycin; neomycin; patents; quantitative polymerase chain reaction; risk; screening; sequence analysis; transferases;     Show all 17 Subjects
Abstract:
... As genetically modified microorganisms (GMM), commonly used by the food and feed industry to produce additives, enzymes and flavourings, are frequently harbouring antimicrobial resistance (AMR) genes as selection markers, health and environmental concerns were consequently raised. For this reason, the interest of the competent authorities to control such microbial fermentation products has strongl ...
DOI:
10.1016/j.ijfoodmicro.2020.108749

18. PM Q-probe: A fluorescent binding protein that converts many antibodies to a fluorescent biosensor

Author:
Dong, Jinhua; Miyake, Chihiro; Yasuda, Takanobu; Oyama, Hiroyuki; Morita, Izumi; Tsukahara, Tomoya; Takahashi, Masaki; Jeong, Hee-Jin; Kitaguchi, Tetsuya; Kobayashi, Norihiro; Ueda, Hiroshi
Source:
Biosensors & bioelectronics 2020 v.165 pp. 112425
ISSN:
0956-5663
Subject:
recombinant DNA,   etc   ; Mycoplasma genitalium; antigens; binding proteins; binding sites; biomarkers; blood serum; cortisol; dose response; fluorescence; fluorescent dyes; humans; immunoglobulin G; immunosensors; recombinant antibodies; thyroxine; tryptophan;     Show all 17 Subjects
Abstract:
... Quenchbody (Q-body) is a fluorescent biosensor in which a fluorescent dye is tagged near the antigen binding site of an antibody. The fluorescence of the dye is quenched by the tryptophan residues present in the variable region of the antibody, and is recovered when the antigen binds. Q-bodies have been prepared using recombinant DNA technology by introducing one or more tag sequence(s) at either ...
DOI:
10.1016/j.bios.2020.112425

19. Alpha‐momorcharin enhances Nicotiana benthamiana resistance to tobacco mosaic virus infection through modulation of reactive oxygen species

Author:
Zhu, Feng; Zhu, Peng‐Xiang; Xu, Fei; Che, Yan‐Ping; Ma, Yi‐Ming; Ji, Zhao‐Lin
Source:
Molecular plant pathology 2020 v.21 no.9 pp. 1212-1226
ISSN:
1464-6722
Subject:
recombinant DNA,   etc   ; Nicotiana benthamiana; Tobacco mosaic virus; antineoplastic activity; fluorescent proteins; gene expression regulation; gene overexpression; genes; genetic engineering; homeostasis; leaves; pathogens; reactive oxygen species; viruses;     Show all 14 Subjects
Abstract:
... Alpha‐momorcharin (α‐MMC), a member of the plant ribosomal inactivating proteins (RIPs) family, has been proven to exhibit important biological properties in animals, including antiviral, antimicrobial, and antitumour activities. However, the mechanism by which α‐MMC increases plant resistance to viral infections remains unclear. To study the effect of α‐MMC on plant viral defence and how α‐MMC in ...
DOI:
10.1111/mpp.12974

20. State-of-the-art strategies and applied perspectives of enzyme biocatalysis in food sector — current status and future trends

Author:
Bilal, Muhammad; Iqbal, Hafiz M. N.
Source:
Critical reviews in food science and nutrition 2020 v.60 no.12 pp. 2052-2066
ISSN:
1549-7852
Subject:
recombinant DNA,   etc   ; biocatalysis; bioprocessing; biotechnology; cost effectiveness; enzymes; flavor; food industry; food processing; food safety; nutrition; shelf life; taste; texture; yeasts;     Show all 15 Subjects
Abstract:
... With the recent progress in biotechnology, a wide variety of novel enzymes with unique physicochemical properties and diverse applications has been introduced, and new application list continues to extend in the future. Enzymes obtained from microorganisms, including bacteria, fungi, yeast are widely applied in numerous food formulations for intensifying their texture and taste. Owing to several d ...
DOI:
10.1080/10408398.2019.1627284