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1. Bacterial genetics and molecular pathogenesis in the age of high throughput DNA sequencing

Author:
Davey, Lauren; Valdivia, Raphael H
Source:
Current opinion in microbiology 2020 v.54 pp. 59-66
ISSN:
1369-5274
Subject:
recombinant DNA,   etc   ; genes; genetic analysis; genotype-phenotype correlation; high-throughput nucleotide sequencing; microbial genetics; microorganisms; mutants; mutation; pathogenesis; pathogens; phylogeny; virulence; virulent strains;     Show all 14 Subjects
Abstract:
... When Stanley Falkow introduced Molecular Koch’s Postulates (Falkow, 1988) as a conceptual framework to identify microbial factors that contributed to disease, he reaffirmed the prominent role that the basic principles of genetic analysis should play in defining genotype-phenotype associations in microbial pathogens. In classical bacterial genetics the nature of mutations is inferred through cis-tr ...
DOI:
10.1016/j.mib.2020.01.007

2. Tag-Specific Affinity Purification of Recombinant Proteins by Using Molecularly Imprinted Polymers

Author:
Gómez-Arribas, Lidia N.; Urraca, Javier L.; Benito-Peña, Elena; Moreno-Bondi, María C.
Source:
Analytical chemistry 2019 v.91 no.6 pp. 4100-4106
ISSN:
1520-6882
Subject:
recombinant DNA,   etc   ; antibodies; cost effectiveness; epitopes; histidine; peptides; polymers; protein tagging; recombinant proteins; resins;     Show all 10 Subjects
Abstract:
... Epitope tagging is widely used to fuse a known epitope to proteins for which no affinity receptor is available by using recombinant DNA technology. One example is FLAG epitope (DYKDDDDK), which provides better purity and recoveries than the favorite poly histidine tag. However, purification requires using anti-FLAG antibody resins, the high cost and nonreusability of which restrict widespread use. ...
DOI:
10.1021/acs.analchem.8b05731

3. Target-specific gene delivery in plant systems and their expression: Insights into recent developments

Author:
Nandy, Debdyuti; Maity, Amrita; Mitra, Arup Kumar
Source:
Journal of biosciences 2020 v.45 no.1 pp. 30
ISSN:
0250-5991
Subject:
recombinant DNA,   etc   ; Agrobacterium radiobacter; biotechnology; carbon nanotubes; crops; drought tolerance; electroporation; gene editing; gene targeting; gene transfer; nucleases; nutritive value; pest resistance; transcription (genetics); transgenes; transport proteins; zinc finger motif;     Show all 17 Subjects
Abstract:
... In order to improve crop plants in terms of their yield, drought resistance, pest resistance, nutritional value, etc., modern agriculture has relied upon plant genetic engineering. Since the advent of recombinant DNA technology, several tools have been used for genetic transformations in plants such as Agrobacterium tumefaciens, virus-mediated gene transfer, direct gene transfer systems such as el ...
DOI:
10.1007/s12038-020-0008-y

4. Induction of somatic embryogenesis in leaf and root explants of Digitalis lanata Ehrh.: Direct and indirect method

Author:
Bhusare, B.P.; John, C.K.; Bhatt, V.P.; Nikam, T.D.
Source:
South African journal of botany 2020 v.130 pp. 356-365
ISSN:
0254-6299
Subject:
recombinant DNA,   etc   ; 2,4-D; Digitalis lanata; callus; culture media; cytokinins; developmental stages; explants; genetic improvement; genetic transformation; heart; indole acetic acid; indole butyric acid; kinetin; leaves; plantlets; scanning electron microscopy; somatic embryogenesis; somatic embryos;     Show all 19 Subjects
Abstract:
... Somatic embryogenesis was reported via direct and indirect method in cardio-tonic plant Digitalis lanata Ehrh. MS basal medium supplemented with cytokinins BAP, Kinetin (Kin) and TDZ, either alone or in combinations with auxins IAA, 2,4-D and IBA,- at different concentrations (00–3.0 mg/l) were studied. The best direct induction of somatic embryogenesis was observed in leaf (100% response with 11. ...
DOI:
10.1016/j.sajb.2020.01.012

5. Potential of transferring transgenic DNA from silkworm to chicken

Author:
Wang, Yumei; Wang, Zili; Guo, Huizhen; Huang, Jing; Li, Xueying; Sun, Qiang; Wang, Bingbing; Xie, Enyu; Jiang, Liang; Xia, Qingyou
Source:
International journal of biological macromolecules 2020 v.142 pp. 311-319
ISSN:
0141-8130
Subject:
recombinant DNA,   etc   ; blood serum; chickens; digesta; digestive tract; duodenum; essential genes; green fluorescent protein; ileum; insect larvae; jejunum; kidneys; liver; males; ovalbumin; polymerase chain reaction; safety assessment; silkworms; superoxide reductase; transgenic insects;     Show all 20 Subjects
Abstract:
... Safety assessment must be conducted before the commercial release of transgenic silkworms. This study was conducted to assess the potential of transferring transgenic DNA from silkworms to other organisms. One hundred hatched male chickens were evenly assigned into 4 groups (T1–4). Groups T1–3 were fed transgenic silkworms P3+5UI with enhanced green fluorescent protein DNA (EGFP) inserted, A4SOR w ...
DOI:
10.1016/j.ijbiomac.2019.09.102

6. 137 Recovery of quail spermatogenesis by donor spermatogonia transplantation

Author:
Vetokh, A. N.; Tomgorova, E. K.; Volkova, L. A.; Volkova, N. A.; Zinovieva, N. A.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 195
ISSN:
1031-3613
Subject:
recombinant DNA,   etc   ; Sertoli cells; blood; body weight; busulfan; cell culture; cell transplantation; drakes; genetic resources; histology; microsatellite repeats; progeny; quails; seminiferous tubules; spermatogenesis; spermatogonia; spermatozoa; statistical analysis; stem cells; transgenesis;     Show all 20 Subjects
Abstract:
... Spermatogonia, the stem cell precursors of male germ cells, are used as convenient biological material for the preservation of genetic resources (cryobanks) and the introduction of recombinant DNA (transgenesis). Donor spermatogonia subsequently differentiate into mature germ cells (spermatozoa), which are used to produce offspring. Our laboratory is investigating methods to improve the efficiency ...
DOI:
10.1071/RDv32n2Ab137

7. Superactive β-galactosidase inclusion bodies

Author:
Flores, Sandra S.; Nolan, Verónica; Perillo, María A.; Sánchez, Julieta M.
Source:
Colloids and surfaces 2019 v.173 pp. 769-775
ISSN:
0927-7765
Subject:
recombinant DNA,   etc   ; beta-galactosidase; colloids; enzyme kinetics; enzyme stability; inclusion bodies; lactose; pH; protein synthesis; temperature; washing;     Show all 11 Subjects
Abstract:
... Bacterial inclusion bodies (IBs) were historically considered one of the major obstacles in protein production through recombinant DNA techniques and conceived as amorphous deposits formed by passive and rather unspecific structures of unfolded proteins aggregates. Subsequent studies demonstrated that IBs contained an important quantity of active protein. In this work, we proved that recombinant β ...
DOI:
10.1016/j.colsurfb.2018.10.049

8. Application of recombinant hemagglutinin proteins as alternative antigen standards for pandemic influenza vaccines

Author:
Choi, Yejin; Kwon, Seong Yi; Oh, Ho Jung; Shim, Sunbo; Chang, Seokkee; Chung, Hye Joo; Kim, Do Keun; Park, Younsang; Lee, Younghee
Source:
Biotechnology letters 2017 v.39 no.9 pp. 1375-1380
ISSN:
0141-5492
Subject:
recombinant DNA,   etc   ; antigens; hemagglutinins; influenza vaccines; pandemic;     Show all 5 Subjects
Abstract:
... OBJECTIVES: The single radial immunodiffusion (SRID) assay, used to quantify hemagglutinin (HA) in influenza vaccines, requires reference reagents; however, because centralized production of reference reagents may slow the emergency deployment of vaccines, alternatives are needed. RESULTS: We investigated the production of HA proteins using recombinant DNA technology, rather than a traditional egg ...
DOI:
10.1007/s10529-017-2372-8

9. A Dengue Vaccine

Author:
Durbin, Anna P.
Source:
Cell 2016 v.166 pp. 1
ISSN:
0092-8674
Subject:
recombinant DNA,   etc   ; dengue; live vaccines;     Show all 3 Subjects
Abstract:
... Denvaxia is the first licensed vaccine for the prevention of dengue. It is a live vaccine developed using recombinant DNA technology. The vaccine is given as three doses over the course of a year and has the potential to prevent hundreds of thousands of hospitalizations each year. ...
DOI:
10.1016/j.cell.2016.06.036

10. Variation in the pH of experimental diets affects the performance of Lymantria dispar asiatica larvae and its gut microbiota

Author:
Zeng, Jian‐Yong; Shi, Jiang‐Hong; Guo, Jia‐Xing; Shi, Zhong‐Bin; Zhang, Guo‐Cai; Zhang, Jie
Source:
Archives of insect biochemistry and physiology 2020 v.103 no.4 pp. e21654
ISSN:
0739-4462
Subject:
recombinant DNA,   etc   ; Acetobacteraceae; Burkholderiaceae; Flavobacteriaceae; Lactococcus; Lymantria dispar; Prevotella; antioxidant activity; antioxidant enzymes; buffers; community structure; digestive enzymes; discriminant analysis; enzyme activity; experimental diets; feces; food intake; forests; insect larvae; intestinal microorganisms; least squares; microbial communities; mitochondria; mortality; pH; phosphates; sequence analysis; weight gain;     Show all 28 Subjects
Abstract:
... To study dietary pH effects on Lymantria dispar asiatica larvae and provide a theoretical basis for its control in different forests, phosphate buffers (PBs) of pH 6, 7, and 8 were used to prepare experimental diets. The diet prepared with pH 6 PB was named as DPB6, with pH 8 PB as DPB8, and with pH 7 PB as DPB7 (control). The dietary pH was 5.00 in DPB6, 6.05 in control, and 6.50 in DPB8. After f ...
DOI:
10.1002/arch.21654

11. Industrial Enzymology: The Next Chapter

Author:
Arbige, Michael V.; Shetty, Jay K.; Chotani, Gopal K.
Source:
Trends in biotechnology 2019 v.37 no.12 pp. 1355-1366
ISSN:
0167-7799
Subject:
recombinant DNA,   etc   ; animal health; bioindustry; biotechnology; commercialization; consumers (people); enzymology; fabrics; human nutrition; laundry; markets; microorganisms; protein engineering; ribosomal DNA; washing;     Show all 15 Subjects
Abstract:
... This review focuses on recent developments in industrial enzymology, protein engineering, and the design and production of microorganisms. We highlight the latest recombinant DNA (rDNA) technology and tools of protein engineering. These advancements are delivering solutions that address the large unmet needs of customers and markets. To illustrate the progress made over the past three decades, sev ...
DOI:
10.1016/j.tibtech.2019.09.010

12. Effects of Ultrafine Bubbles on Gram-Negative Bacteria: Inhibition or Selection?

Author:
Luu, Trong Quan; Hong Truong, Phung Ngoc; Zitzmann, Kim; Nguyen, Khoi Tan
Source:
Langmuir 2019 v.35 no.42 pp. 13761-13768
ISSN:
1520-5827
Subject:
recombinant DNA,   etc   ; Escherichia coli; Fourier transform infrared spectroscopy; Gram-negative bacteria; Pseudomonas aeruginosa; absorbance; bacterial culture; bubbles; cell growth; fluorescence microscopy; industrial applications; nanobubbles; phenotype; spectral analysis;     Show all 14 Subjects
Abstract:
... Ultrafine bubbles exist in all liquids and are naturally stable. As their properties are not entirely known, it is unclear how they impact the surrounding solution and comparable-sized particles within it. It is essential to further investigate the properties of ultrafine bubbles in order to expand their industrial application. In this regard, the effect of ultrafine bubbles on bacterial developme ...
DOI:
10.1021/acs.langmuir.9b02641

13. Metabolic Engineering of Bacteria for Renewable Bioethanol Production from Cellulosic Biomass

Author:
Banerjee, Sanchita; Mishra, Gargi; Roy, Amit
Source:
Biotechnology and bioprocess engineering 2019 v.24 no.5 pp. 713-733
ISSN:
1226-8372
Subject:
recombinant DNA,   etc   ; alcohols; bacteria; biochemical pathways; bioethanol; biomass; bioprocessing; enzymes; ethanol production; fermentation; fossil fuels; fuel production; genes; hydrolysis; metabolic engineering; raw materials; sugars;     Show all 17 Subjects
Abstract:
... Biomass is renewable and most abundant raw material on earth. Technologies have been employed to utilize this vast renewable resource for biofuel production. In recent years, microorganisms have received much attention for biofuel production so that it can replace the non-renewable fossil fuels. They secrete synergistic enzymes for degrading the complex biomass to simple sugars and subsequently fe ...
DOI:
10.1007/s12257-019-0134-2

14. Biochemical Characteristics of Microbial Enzymes and Their Significance from Industrial Perspectives

Author:
Thapa, Santosh; Li, Hui; OHair, Joshua; Bhatti, Sarabjit; Chen, Fur-Chi; Nasr, Kamal Al; Johnson, Terrance; Zhou, Suping
Source:
Molecular biotechnology 2019 v.61 no.8 pp. 579-601
ISSN:
1073-6085
Subject:
recombinant DNA,   etc   ; biofuels; detergents; drugs; fabrics; feed processing; genetic engineering; high-throughput nucleotide sequencing; manufacturing; microbial enzymes; microorganisms; pH stability; paper; polymers; proteinases; therapeutics; thermal stability;     Show all 17 Subjects
Abstract:
... Microbes are ubiquitously distributed in nature and are a critical part of the holobiont fitness. They are perceived as the most potential biochemical reservoir of inordinately diverse and multi-functional enzymes. The robust nature of the microbial enzymes with thermostability, pH stability and multi-functionality make them potential candidates for the efficient biotechnological processes under d ...
DOI:
10.1007/s12033-019-00187-1

15. Adjuvanted leptospiral vaccines: Challenges and future development of new leptospirosis vaccines

Author:
Teixeira, Aline F.; Fernandes, Luis G.V.; Cavenague, Maria F.; Takahashi, Maria B.; Santos, Jademilson C.; Passalia, Felipe J.; Daroz, Brenda B.; Kochi, Leandro T.; Vieira, Monica L.; Nascimento, Ana L.T.O.
Source:
Vaccine 2019 v.37 no.30 pp. 3961-3973
ISSN:
0264-410X
Subject:
recombinant DNA,   etc   ; Leptospiraceae; adjuvants; aluminum; antigen-presenting cells; antigens; at-risk population; humans; immune response; leptospirosis; oils; recombinant vaccines; salts; vaccination; China; Cuba; France;     Show all 17 Subjects
Abstract:
... Leptospirosis is a neglected infectious disease of global importance. Vaccination is the most viable strategy for the control of leptospirosis, but in spite of efforts for the development of an effective vaccine against the disease, few advances have been made, and to date, bacterin is the only option for prevention of leptospirosis. Bacterins are formulations based on inactivated leptospires that ...
DOI:
10.1016/j.vaccine.2019.05.087

16. Structural studies on dihydrouridine synthase A (DusA) from Pseudomonas aeruginosa

Author:
Goyal, Nainee; Chandra, Anshuman; Qamar, Imteyaz; Singh, Nagendra
Source:
International journal of biological macromolecules 2019 v.132 pp. 254-264
ISSN:
0141-8130
Subject:
recombinant DNA,   etc   ; Pseudomonas aeruginosa; catalytic activity; circular dichroism spectroscopy; drug design; enzymes; genes; monitoring; puromycin; temperature; transfer RNA; translation (genetics); urea; uridine;     Show all 14 Subjects
Abstract:
... Dihydrouridination is one of the abundant modifications in tRNA editing. The presence of dihydrouridine is attributed to tRNA stability desired for the efficient gene translation process. The conversion of uridine to dihydrouridine is catalyzed by flavine containing enzyme called dihydrouridine synthase (Dus). We report first ever information about DusA enzyme from Pseudomonas aeruginosa in form o ...
DOI:
10.1016/j.ijbiomac.2019.03.209

17. Effects of recombinant goose adiponectin on steroid hormone secretion in Huoyan geese ovarian granulosa cells

Author:
Meng, Bo; Cao, Zhongzan; Gai, Yedan; Liu, Mei; Gao, Ming; Chen, Meiyue; Ning, Zhili; Luan, Xinhong
Source:
Animal reproduction science 2019 v.205 pp. 34-43
ISSN:
0378-4320
Subject:
recombinant DNA,   etc   ; adiponectin; aromatase; cholesterol; estradiol; fluorescence; geese; granulosa cells; hormone secretion; messenger RNA; progesterone; steroid hormones; steroidogenesis; steroidogenic acute regulatory protein;     Show all 14 Subjects
Abstract:
... Adiponectin is an adipokine associated with the regulation of reproductive processes. To determine whether recombinant goose adiponectin contributes to the goose ovarian steroidogenesis, the native and then purified goose recombinant adiponectin protein was produced using recombinant DNA technologies. The effect of recombinant adiponectin on the progesterone (P4) and estradiol (E2) production in H ...
DOI:
10.1016/j.anireprosci.2019.03.019

18. Occurrence of tomato leaf curl Bangladesh virus and associated subviral DNA molecules in papaya in Bangladesh: molecular detection and characterization

Author:
Hamim, Islam; Borth, Wayne B.; Melzer, Michael J.; Suzuki, Jon Y.; Wall, Marisa M.; Hu, John S.
Source:
Archives of virology 2019 v.164 no.6 pp. 1661-1665
ISSN:
0304-8608
Subject:
recombinant DNA,   etc   ; Tomato leaf curl Bangladesh virus; Tomato leaf curl betasatellite; leaf curling; oligodeoxyribonucleotides; polymerase chain reaction; satellite DNA; viruses; Bangladesh;     Show all 9 Subjects
Abstract:
... Forty-five papaya samples showing severe leaf curl symptoms were tested by PCR with a degenerate primer set for virus species in the genus Begomovirus. Of these, 29 were positive for tomato leaf curl Bangladesh virus (ToLCBV). The complete genome sequences of ToLCBV (GenBank accession no. MH380003) and its associated tomato leaf curl betasatellite (ToLCB) (MH397223) from papaya isolate Gaz17-Pap w ...
DOI:
10.1007/s00705-019-04235-8
CHORUS:
10.1007/s00705-019-04235-8

19. Recombinant Human Hair Keratin Nanoparticles Accelerate Dermal Wound Healing

Author:
Gao, Feiyan; Li, Wenfeng; Deng, Jia; Kan, Jinlan; Guo, Tingwang; Wang, Bochu; Hao, Shilei
Source:
ACS applied materials & interfaces 2019 v.11 no.20 pp. 18681-18690
ISSN:
1944-8252
Subject:
recombinant DNA,   etc   ; biocompatibility; biocompatible materials; cell proliferation; circular dichroism spectroscopy; collagen; gel electrophoresis; hairs; humans; keratin; mass spectrometry; molecular weight; nanoparticles; scanning electron microscopes; tissue repair; toxicity; ultrasonics;     Show all 17 Subjects
Abstract:
... In recent years, favorable enhanced wound-healing properties and excellent biocompatibility of keratin derived from human hair have attracted considerable attention. Recombinant keratin proteins can be produced by recombinant DNA technology and have higher purity than extracted keratin. However, the wound-healing properties of recombinant keratin proteins remain unclear. Herein, two recombinant tr ...
DOI:
10.1021/acsami.9b01725

20. Evaluation of the expression stability of β-actin under bacterial infection in Macrobrachium nipponense

Author:
Geng, Wen-Yi; Yao, Feng-Jiao; Tang, Ting; Shi, Shan-Shan
Source:
Molecular biology reports 2019 v.46 no.1 pp. 309-315
ISSN:
0301-4851
Subject:
recombinant DNA,   etc   ; Aeromonas hydrophila; Escherichia coli; Macrobrachium nipponense; actin; bacteria; bacterial infections; bioinformatics; databases; gene expression; genes; genetic vectors; nickel; phylogeny; quantitative polymerase chain reaction; shrimp;     Show all 16 Subjects
Abstract:
... The selection of a suitable reference gene is an important prerequisite for the precise analysis of target gene expression by real-time quantitative PCR (qPCR). The present study aims to explore the expression pattern of the Macrobrachium nipponense (M. nipponense) β-actin gene under Aeromonas hydrophila bacterial infection conditions. The complete sequence of the β-actin gene from M. nipponense w ...
DOI:
10.1007/s11033-018-4473-4