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You searched for: Subject "recombinant proteins" Remove constraint Subject: "recombinant proteins"
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1. Transcriptome analysis and pattern recognition receptors (PRRs) identification in different tissues of adult Pacific oysters infected with Vibrio parahaemolyticus

Author:
Petcharat Namwong; Shuang Wang; Qing Kong; Haijin Mou; Lei Ma; Prapansak Srisapoome
Source:
Aquaculture 2023 v.562 pp. 738824
ISSN:
0044-8486
Subject:
recombinant proteins,   etc   ; Crassostrea gigas; Gram-negative bacteria; Vibrio parahaemolyticus; adults; aquaculture industry; bacterial adhesion; bacterial infections; carbohydrate binding; cytoskeleton; data collection; epithelium; fluorescent antibody technique; food sanitation; galectins; gene expression; gene expression regulation; genes; host-pathogen relationships; macrophages; mannose; oxidative stress; pathogens; peptidoglycans; peroxidase; phagocytosis; shellfish culture; transcriptomics;     Show all 28 Subjects
Abstract:
... Contamination of V. parahaemolyticus in raw product and culture areas of Crassostrea gigas has been a concern in food hygiene and infectious diseases in the shellfish aquaculture industry for decades. Although numerous researchers have presently objective of decreasing pathogen microorganism contaminated load in raw oysters or genetics improvement for prevention, the in-depth information at the mo ...
DOI:
10.1016/j.aquaculture.2022.738824

2. Progress toward rapid, at-line N-glycosylation detection and control for recombinant protein expression

Author:
Leran Mao; James W Schneider; Anne S Robinson
Source:
Current opinion in biotechnology 2022 v.78 pp. 102788
ISSN:
0958-1669
Subject:
recombinant proteins,   etc   ; biotechnology; glycoproteins; glycosylation; markets; therapeutics;     Show all 6 Subjects
Abstract:
... Proteins continue to represent a large fraction of the therapeutics market, reaching over a hundred billion dollars in market size globally. One key feature of protein modification that can affect both structure and function is the addition of glycosylation following protein folding, leading to regulatory requirements for the accurate assessment of protein attributes, including glycan structures. ...
DOI:
10.1016/j.copbio.2022.102788

3. Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins

Author:
Magdalena Malm; Chih-Chung Kuo; Mona Moradi Barzadd; Aman Mebrahtu; Num Wistbacka; Ronia Razavi; Anna-Luisa Volk; Magnus Lundqvist; David Kotol; Hanna Tegel; Sophia Hober; Fredrik Edfors; Torbjörn Gräslund; Veronique Chotteau; Ray Field; Paul G. Varley; Robert G. Roth; Nathan E. Lewis; Diane Hatton; Johan Rockberg
Source:
Metabolic engineering 2022 v.72 pp. 171-187
ISSN:
1096-7176
Subject:
recombinant proteins,   etc   ; glycosylation; humans; secretion; therapeutics; transcriptome; transcriptomics;     Show all 7 Subjects
Abstract:
... Biologics represent the fastest growing group of therapeutics, but many advanced recombinant protein moieties remain difficult to produce. Here, we identify metabolic engineering targets limiting expression of recombinant human proteins through a systems biology analysis of the transcriptomes of CHO and HEK293 during recombinant expression. In an expression comparison of 24 difficult to express pr ...
DOI:
10.1016/j.ymben.2022.03.009

4. A trimeric immunoglobin G‐binding domain outperforms recombinant protein G and protein L as a ligand for fragment antigen‐binding purification

Author:
Hongyu Lu; Youmei Jin; Hao Yang; Ze Tao; Jie Chen; Shiyuan Chen; Yanru Feng; Huimin Xing; Xiaofeng Lu
Source:
Journal of chromatography 2022 v.1681 pp. 463464
ISSN:
0021-9673
Subject:
recombinant proteins,   etc   ; Escherichia coli; affinity chromatography; humans; ligands; rats;     Show all 6 Subjects
Abstract:
... Fragment antigen-binding (Fab) has several advantages in the treatment and diagnosis of some diseases. The lack of highly efficient affinity chromatography platform creates a purification bottleneck for the downstream processing of Fab-based products, which raises the urgent need for a novel immunoglobin G (IgG)-binding domain (IgBD) with both high affinity and broad specificity for Fab. SpGC₃FₐbR ...
DOI:
10.1016/j.chroma.2022.463464

5. Extremely low frequency magnetic field enhances expression of a specific recombinant protein in bacterial host

Author:
Behnaz Rashidieh; Alireza Madjid Ansari; Mahdi Behdani; Behrad Darvishi; Mahdi Habibi-Anbouhi
Source:
Analytical biochemistry 2022 v.652 pp. 114745
ISSN:
0003-2697
Subject:
recombinant proteins,   etc   ; heterologous gene expression; industry; magnetic fields; protein synthesis;     Show all 5 Subjects
Abstract:
... Expression of proteins in bacterial host cells, particularly E.coli, has gained much attention in recent years. Low expression outcome is the main technical drawback associated with this procedure, further restricting its largescale application in industry. Therefore, application of new amendments or reformations are required before further proceedings. Extremely low frequency magnetic fields (ELF ...
DOI:
10.1016/j.ab.2022.114745

6. PERISCOPE-Opt: Machine learning-based prediction of optimal fermentation conditions and yields of recombinant periplasmic protein expressed in Escherichia coli

Author:
Kulandai Arockia Rajesh Packiam; Chien Wei Ooi; Fuyi Li; Shutao Mei; Beng Ti Tey; Huey Fang Ong; Jiangning Song; Ramakrishnan Nagasundara Ramanan
Source:
Computational and Structural Biotechnology Journal 2022 v.20 pp. 2909-2920
ISSN:
2001-0370
Subject:
recombinant proteins,   etc   ; Escherichia coli; biotechnology; fermentation; models; prediction; protein synthesis;     Show all 7 Subjects
Abstract:
... Optimization of the fermentation process for recombinant protein production (RPP) is often resource-intensive. Machine learning (ML) approaches are helpful in minimizing the experimentations and find vast applications in RPP. However, these ML-based tools primarily focus on features with respect to amino-acid-sequence, ruling out the influence of fermentation process conditions. The present study ...
DOI:
10.1016/j.csbj.2022.06.006

7. Intermittent induction of LEA peptide by lactose enhances the expression of insecticidal proteins in Bacillus thuringiensis

Author:
Mahmuda Akthar; Tomoko Shimokawa; Yinghan Wu; Taichi Arita; Kazuhiro Mizuta; Yuria Isono; Minoru Maeda; Shinya Ikeno
Source:
FEBS Open Bio 2022 v.12 no.8 pp. 1534-1541
ISSN:
2211-5463
Subject:
recombinant proteins,   etc   ; Bacillus thuringiensis; bacterial proteins; embryogenesis; lactose; peptides; protein synthesis;     Show all 7 Subjects
Abstract:
... Cry toxins from Bacillus thuringiensis (Bt) have been extensively applied in agriculture to substitute the use of chemical insecticides. We have previously reported the use of a coexpression system in which late embryogenesis abundant (LEA) peptides under the control of the lac promoter increase the expression of insecticidal proteins in Bt. The use of lactose to induce the expression of LEA pepti ...
DOI:
10.1002/2211-5463.13448

8. The potential of emerging sub-omics technologies for CHO cell engineering

Author:
Tobias Jerabek; Christoph Keysberg; Kerstin Otte
Source:
Biotechnology advances 2022 v.59 pp. 107978
ISSN:
0734-9750
Subject:
recombinant proteins,   etc   ; Cricetulus griseus; biotechnology; cell lines; mass spectrometry; protein synthesis;     Show all 6 Subjects
Abstract:
... Chinese hamster ovary (CHO) cells have been the predominant host for recombinant protein production over the past decades with major efforts directed towards cell line engineering to increase amount and quality of biopharmaceutics. Emerging omics approaches and corresponding techniques now allow an extensive characterization of cellular expression systems. Technical improvements in sequencing, mas ...
DOI:
10.1016/j.biotechadv.2022.107978

9. Effect of fruit maturation on N-glycosylation of plant-derived native and recombinant miraculin

Author:
Hiroyuki Kajiura; Kyoko Hiwasa-Tanase; Hiroshi Ezura; Kazuhito Fujiyama
Source:
Plant physiology and biochemistry 2022 v.178 pp. 70-79
ISSN:
0981-9428
Subject:
recombinant proteins,   etc   ; Synsepalum dulcificum; fruits; glycosylation; peptides; plant physiology; protein sweeteners; tomatoes;     Show all 8 Subjects
Abstract:
... Miracle fruit, Synsepalum dulcificum, produces a unique taste-modifying protein, miraculin (MIR), which has an attractive potential for commercial application as a novel low-calorie sweetener. To establish a stable supply system for MIR, a previous study established a platform for recombinant MIR (rMIR) production in tomato plants and demonstrated that native miraculin from miracle fruit (nMIR) an ...
DOI:
10.1016/j.plaphy.2022.02.026

10. Bacterial over-expression of functionally active human CT2 (SLC22A16) carnitine transporter

Author:
Michele Galluccio; Tiziano Mazza; Mariafrancesca Scalise; Maria Chiara Sarubbi; Cesare Indiveri
Source:
Molecular biology reports 2022 v.49 no.8 pp. 8185-8193
ISSN:
0301-4851
Subject:
recombinant proteins,   etc   ; Escherichia coli; carnitine; glucose; human health; humans; molecular biology; toxicity;     Show all 8 Subjects
Abstract:
... BACKGROUND: Escherichia coli is a widely used tool for the over-expression of human proteins for studying structure and function. The toxicity of human proteins for E. coli often hampers the expression. This study aims to find conditions for the expression of a membrane transporter known as the carnitine transporter CT2. The knowledge on this transporter is scarce, thus obtaining the recombinant p ...
DOI:
10.1007/s11033-022-07491-1

11. Comparison of the multi-epitope recombinant antigen DIPOL and hydatid fluid for the diagnosis of patients with cystic echinococcosis

Author:
Eylem Akdur Ozturk; Raúl Manzano-Román; Carlos Sánchez-Ovejero; Ayse Caner; Mesude Angın; Cumhur Gunduz; Ülkü Karaman; Nuray Altintas; Halil Bozkaya; Omer Unalp; Zafer Dokumcu; Emre Divarci; Adriano Casulli; Nazmiye Altintas; Mar Siles-Lucas; Aysegul Unver
Source:
Acta tropica 2022 v.225 pp. 106208
ISSN:
0001-706X
Subject:
recombinant proteins,   etc   ; blood serum; disease diagnosis; echinococcosis; genotype; genotyping; immunodominant epitopes; parasites;     Show all 8 Subjects
Abstract:
... The use of serological tests containing multiple immunodominant antigens rather than single antigens have the potential to improve the diagnostic performance in Cystic Echinococcoses (CE) as a complement tool to clear the inconclusive imaging data. Here, we comparatively evaluated the diagnostic value of Hydatid Fluid (HF) and the recently described recombinant multi-epitope antigen DIPOL in IgG-E ...
DOI:
10.1016/j.actatropica.2021.106208

12. Cross-identification of N-Glycans by CE-LIF using two capillary coatings and three labeling dyes

Author:
Pingjing Li; Lulu Wang; Rui Guo; Huatao Feng; Ya Ji; Si Ying Lim; Bao Hui Ng; Anna Karen Carrasco Laserna; Shaheer Khan; Shiaw-Min Chen; Sam Fong Yau Li
Source:
Talanta 2022 v.239 pp. 123061
ISSN:
0039-9140
Subject:
recombinant proteins,   etc   ; biopharmaceuticals; covariance; drug development; fluorescence; glycosylation; landscapes; maltodextrins; polyvinyl alcohol;     Show all 9 Subjects
Abstract:
... Recombinant protein biopharmaceuticals comprise a significant portion of the current drug development landscape. The glycosylation profile of these proteins is a key quality parameter as it can affect their safety, efficacy, and stability. However, glycan analysis is challenging because of the complexity of their structures. To overcome this challenge in achieving accurate glycan identification, c ...
DOI:
10.1016/j.talanta.2021.123061

13. Pushing and pulling proteins into the yeast secretory pathway enhances recombinant protein secretion

Author:
Richard J. Zahrl; Roland Prielhofer; Özge Ata; Kristin Baumann; Diethard Mattanovich; Brigitte Gasser
Source:
Metabolic engineering 2022 v.74 pp. 36-48
ISSN:
1096-7176
Subject:
recombinant proteins,   etc   ; Komagataella pastoris; antibodies; bioreactors; cytosol; endoplasmic reticulum; protein secretion; yeasts;     Show all 8 Subjects
Abstract:
... Yeasts and especially Pichia pastoris (syn Komagataella spp.) are popular microbial expression systems for the production of recombinant proteins. One of the key advantages of yeast host systems is their ability to secrete the recombinant protein into the culture media. However, secretion of some recombinant proteins is less efficient. These proteins include antibody fragments such as Fabs or scFv ...
DOI:
10.1016/j.ymben.2022.08.010

14. A Five-Helix-Based SARS-CoV-2 Fusion Inhibitor Targeting Heptad Repeat 2 Domain against SARS-CoV-2 and Its Variants of Concern

Author:
Lixiao Xing; Xinfeng Xu; Wei Xu; Zezhong Liu; Xin Shen; Jie Zhou; Ling Xu; Jing Pu; Chan Yang; Yuan Huang; Lu Lu; Shibo Jiang; Shuwen Liu
Source:
Viruses 2022 v.14 no.3 pp. -
ISSN:
1999-4915
Subject:
recombinant proteins,   etc   ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; pandemic; therapeutics;     Show all 5 Subjects
Abstract:
... The prolonged duration of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) pandemic has resulted in the continuous emergence of variants of concern (VOC, e.g., Omicron) and variants of interest (VOI, e.g., Lambda). These variants have challenged the protective efficacy of current COVID-19 vaccines, thus calling for the development of novel therapeutics against SARS-CoV-2 and its VO ...
DOI:
10.3390/v14030597

15. Production of recombinant and therapeutic proteins in microalgae

Author:
Anirban Banerjee; Valerie Ward
Source:
Current opinion in biotechnology 2022 v.78 pp. 102784
ISSN:
0958-1669
Subject:
recombinant proteins,   etc   ; biobased products; biotechnology; dietary supplements; drug carriers; drugs; microalgae; protein synthesis;     Show all 8 Subjects
Abstract:
... Microalgae is emerging as a promising platform for the production of recombinant proteins of therapeutic importance. Since microalgae are safe for oral consumption and some microalgal species can form extracellular vesicles, they can potentially be used as edible vaccines or drug-delivery vehicles in addition to their status as naturally rich sources of nutraceutical bioproducts. This article focu ...
DOI:
10.1016/j.copbio.2022.102784

16. Characterization of novel endo-β-N-acetylglucosaminidase from Bacteroides nordii that hydrolyzes multi-branched complex type N-glycans

Author:
Kristina Mae Bienes; Feunai Agape Papalii Tautau; Ai Mitani; Takashi Kinoshita; Shin-ichi Nakakita; Yujiro Higuchi; Kaoru Takegawa
Source:
Journal of bioscience and bioengineering 2022 v.134 no.1 pp. 7-13
ISSN:
1389-1723
Subject:
recombinant proteins,   etc   ; Bacteroides; Escherichia coli; fetuins; galactose; glycoproteins; humans; oligosaccharides; pH; sialic acid; transferrin;     Show all 11 Subjects
Abstract:
... Endo-β-N-acetylglucosaminidases (ENGases) are enzymes that hydrolyze the N-linked oligosaccharides. Many ENGases have already been identified and characterized. However, there are still a few enzymes that have hydrolytic activity toward multibranched complex-type N-glycans on glycoproteins. In this study, one novel ENGase from Bacteroides nordii (Endo-BN) species was identified and characterized. ...
DOI:
10.1016/j.jbiosc.2022.03.011

17. Optimised expression and purification of RBP4 and preparation of anti‐RBP4 monoclonal antibody

Author:
Hui Li; Xiao He; Sha Wen; Lichao Yang; Qiuli Chen; Yasi Li; Shiping Huang; Xuejing Huang; Fengjie Wan; Min He
Source:
FEBS Open Bio 2022 v.12 no.2 pp. 430-442
ISSN:
2211-5463
Subject:
recombinant proteins,   etc   ; Escherichia coli; hepatoma; hybridomas; immunohistochemistry; liver; monoclonal antibodies; prognosis; retinol-binding protein;     Show all 9 Subjects
Abstract:
... The expression level of retinol‐binding protein 4 (RBP4) protein is closely related to liver damage and plays an important role in the diagnosis and prognosis of cancer. However, the preparation of anti‐RBP4 mAb or exploration on the application of anti‐RBP4 mAb has not been reported thus far. In the present study, we constructed a pET30a‐RBP4 recombinant vector, used E. coli BL21 (DE3) as the vec ...
DOI:
10.1002/2211-5463.13349

18. Selective cleavage of ncRNA and antiviral activity by RNase2/EDN in THP1-induced macrophages

Author:
Lu Lu; Jiarui Li; Ranlei Wei; Irene Guidi; Luca Cozzuto; Julia Ponomarenko; Guillem Prats-Ejarque; Ester Boix
Source:
Cellular and molecular life sciences 2022 v.79 no.4 pp. 209
ISSN:
1420-682X
Subject:
recombinant proteins,   etc   ; Respiratory syncytial virus; antiviral properties; cell viability; macrophages; microRNA; non-coding RNA;     Show all 7 Subjects
Abstract:
... RNase2 is the member of the RNaseA family most abundant in macrophages. Here, we knocked out RNase2 in THP-1 cells and analysed the response to Respiratory Syncytial Virus (RSV). RSV induced RNase2 expression, which significantly enhanced cell survival. Next, by cP-RNAseq sequencing, which amplifies the cyclic-phosphate endonuclease products, we analysed the ncRNA population. Among the ncRNAs accu ...
DOI:
10.1007/s00018-022-04229-x

19. LIKE EARLY STARVATION 1 alters the glucan structures at the starch granule surface and thereby influences the action of both starch‐synthesizing and starch‐degrading enzymes

Author:
Aakanksha Singh; Julia Compart; Shadha Abduljaleel AL‐Rawi; Harendra Mahto; Abubakar Musa Ahmad; Joerg Fettke
Source:
plant journal 2022 v.111 no.3 pp. 819-835
ISSN:
0960-7412
Subject:
recombinant proteins,   etc   ; Arabidopsis; additive effect; isoamylase; metabolism; phosphorylase; phosphorylation; sequence homology; starch granules; starvation;     Show all 10 Subjects
Abstract:
... For starch metabolism to take place correctly, various enzymes and proteins acting on the starch granule surface are crucial. Recently, two non‐catalytic starch‐binding proteins, pivotal for normal starch turnover in Arabidopsis leaves, namely, EARLY STARVATION 1 (ESV1) and its homolog LIKE EARLY STARVATION 1 (LESV), have been identified. Both share nearly 38% sequence homology. As ESV1 has been f ...
DOI:
10.1111/tpj.15855

20. Comparative analysis of mussel foot protein 3B co-expressed with tyrosinases provides a potential adhesive biomaterial

Author:
Lin Yao; Xinyi Wang; Rui Xue; Hong Xu; Rui Wang; Lujia Zhang; Sha Li
Source:
International journal of biological macromolecules 2022 v.195 pp. 229-236
ISSN:
0141-8130
Subject:
recombinant proteins,   etc   ; Escherichia coli; Mytilus galloprovincialis; Verrucomicrobium spinosum; biocompatible materials; dihydroxyphenylalanine; hydroxylation; mussels; spectroscopy; tyrosine;     Show all 10 Subjects
Abstract:
... Mussel foot proteins (Mfps), which help mussels attach to various surfaces, are considered to be promising biomaterials due to their outstanding adhesive properties. However, limited production and lack of post-translational modifications of tyrosine residues into 3,4-dihydroxyphenylalanine (Dopa) in bacterial expression systems have hampered their applications. In the present study, for the first ...
DOI:
10.1016/j.ijbiomac.2021.11.208