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1. Novel association of SNP rs2297828 in PRDM16 gene with predisposition to type 2 diabetes

Author:
Hongmei Zhang; Qiuyue Guan; Ruyi Wang; Shanshan Yang; Xiaoqian Yu; Daxin Cui; Zhiguang Su
Source:
Gene 2023 v.849 pp. 146916
ISSN:
0378-1119
Subject:
reporter genes,   etc   ; alleles; blood; energy; genetic variation; glucose; heterozygosity; homeostasis; homozygosity; lipid metabolism; luciferase; models; noninsulin-dependent diabetes mellitus; promoter regions; risk; transcription factors;     Show all 16 Subjects
Abstract:
... Transcriptional regulator PRD1-BF-1-RIZ1 homology (PR) domain containing protein-16 (PRDM16) has a fundamental function in maintaining energy homeostasis and regulating glucose and lipid metabolism, which are responsible for the development of type 2 diabetes (T2D). However, the impact of genetic variation of PRDM16 gene on T2D risk remains to be investigated. Thus, we evaluated the possible assoc ...
DOI:
10.1016/j.gene.2022.146916

2. The Escherichia coli Amino Acid Uptake Protein CycA: Regulation of Its Synthesis and Practical Application in l-Isoleucine Production

Author:
Christine Hook; Natalya Eremina; Petr Zaytsev; Daria Varlamova; Nataliya Stoynova
Source:
Microorganisms 2022 v.10 no.3 pp. -
ISSN:
2076-2607
Subject:
reporter genes,   etc   ; Escherichia coli; gel electrophoresis;     Show all 3 Subjects
Abstract:
... Amino acid transport systems perform important physiological functions; their role should certainly be considered in microbial production of amino acids. Typically, in the context of metabolic engineering, efforts are focused on the search for and application of specific amino acid efflux pumps. However, in addition, importers can also be used to improve the industrial process as a whole. In this ...
DOI:
10.3390/microorganisms10030647

3. Translational repression of FZP mediated by CU‐rich element/OsPTB interactions modulates panicle development in rice

Author:
Qiong Chen; Fa'an Tian; Tingting Cheng; Jun'e Jiang; Guanlin Zhu; Zhenyu Gao; Haiyan Lin; Jiang Hu; Qian Qian; Xiaohua Fang; Fan Chen
Source:
plant journal 2022 v.110 no.5 pp. 1319-1331
ISSN:
0960-7412
Subject:
reporter genes,   etc   ; panicles; rice; transcription (genetics);     Show all 4 Subjects
Abstract:
... Panicle development is an important determinant of the grain number in rice. A thorough characterization of the molecular mechanism underlying panicle development will lead to improved breeding of high‐yielding rice varieties. Frizzy Panicle (FZP), a critical gene for panicle development, is regulated by OsBZR1 and OsARFs at the transcriptional stage. However, the translational modulation of FZP h ...
DOI:
10.1111/tpj.15737

4. Identification of Cryptic Promoter Activity in cDNA Sequences Corresponding to PRRSV 5′ Untranslated Region and Transcription Regulatory Sequences

Author:
Jayeshbhai Chaudhari; The Nhu Nguyen; Hiep L. X. Vu
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
reporter genes,   etc   ; RNA replication; luciferase; mutants; plasmids; viruses;     Show all 6 Subjects
Abstract:
... To investigate the role of PRRSV nonstructural proteins (nsps) in viral RNA replication and transcription, we generated a cDNA clone of PRRSV strain NCV1 carrying the nanoluciferase (nluc) gene under the control of the transcription regulatory sequence 6 (TRS6) designated as pNCV1-Nluc. Cells transfected with the pNCV1-Nluc DNA plasmid produced an infectious virus and high levels of luciferase act ...
DOI:
10.3390/v14020400

5. Establishment of a Cre-loxP System Based on a Leaky LAC4 Promoter and an Unstable panARS Element in Kluyveromyces marxianus

Author:
Haiyan Ren; Anqi Yin; Pingping Wu; Huanyu Zhou; Jungang Zhou; Yao Yu; Hong Lu
Source:
Microorganisms 2022 v.10 no.6 pp. -
ISSN:
2076-2607
Subject:
reporter genes,   etc   ; Kluyveromyces marxianus; centromeres; cytotoxicity; plasmids; recombinases;     Show all 6 Subjects
Abstract:
... The Cre-loxP system produces structural variations, such as deletion, duplication, inversion and translocation, at specific loci and induces chromosomal rearrangements in the genome. To achieve chromosomal rearrangements in Kluyveromyces marxianus, the positions and sequences of centromeres were identified in this species for the first time. Next, a Cre-loxP system was established in K. marxianus. ...
DOI:
10.3390/microorganisms10061240

6. I-SceI and customized meganucleases-mediated genome editing in tomato and oilseed rape

Author:
Benoit Danilo; Émilie Montes; Héloïse Archambeau; Maryse Lodé; Mathieu Rousseau-Gueutin; Anne-Marie Chèvre; Marianne Mazier
Source:
Transgenic research 2022 v.31 no.1 pp. 87-105
ISSN:
0962-8819
Subject:
reporter genes,   etc   ; Brassica napus; DNA; excision; tomatoes; toxicity; transgenes;     Show all 7 Subjects
Abstract:
... Meganucleases are rare cutting enzymes that can generate DNA modifications and are part of the plant genome editing toolkit although they lack versatility. Here, we evaluated the use of two meganucleases, I-SceI and a customized meganuclease, in tomato and oilseed rape. Different strategies were explored for the use of these meganucleases. The activity of a customized and a I-SceI meganucleases wa ...
DOI:
10.1007/s11248-021-00287-2

7. Development of retinoic acid receptor antagonists bearing trans-SF4-alkynyl structure as a linear linker

Author:
Shuichi Mori; Nozomi Tsuemoto; Emiko Kawachi; Chiharu Takubo; Aya Tanatani; Hiroyuki Kagechika
Source:
Tetrahedron 2022 pp. 132967
ISSN:
0040-4020
Subject:
reporter genes,   etc   ; chlorides; hydrochloric acid; moieties; retinoic acid receptors; retinoids;     Show all 6 Subjects
Abstract:
... Tetrafluoro-λ⁶-sulfane (tetrafluorosulfanyl, SF₄) compounds possess unique properties, including a linear structure and high electronegativity. Here, to investigate the utility of the trans-SF₄ moiety as a structural element of bioactive compounds, we designed and synthesized novel retinoids containing a trans-SF₄-alkynyl group as a linear linker. The radical addition reaction of aryltetrafluorosu ...
DOI:
10.1016/j.tet.2022.132967

8. Teratogenicity of retinoids detected in surface waters in zebrafish embryos and its predictability by in vitro assays

Author:
Marek Pípal; Jiří Novák; Aneta Rafajová; Marie Smutná; Klára Hilscherová
Source:
Aquatic toxicology 2022 v.246 pp. 106151
ISSN:
0166-445X
Subject:
reporter genes,   etc   ; Danio rerio; embryotoxicity; retinoic acid; teratogenicity; teratogens; toxicology;     Show all 7 Subjects
Abstract:
... Retinoids are newly detected compounds in aquatic ecosystems associated with cyanobacterial water blooms. Their potential health risks are only scarcely described despite numerous detections of all-trans retinoic acid (ATRA) and its derivatives in the environment. Besides the known teratogen ATRA there is only little or no information about their potency and namely their effects in vivo. We charac ...
DOI:
10.1016/j.aquatox.2022.106151

9. Dechloranes exhibit binding potency and activity to thyroid hormone receptors

Author:
Jianqiao Zhu; Lixia Zhao; Lianghong Guo
Source:
Journal of environmental sciences (China) 2022 v.112 pp. 16-24
ISSN:
1001-0742
Subject:
reporter genes,   etc   ; fluorescence; humans; luciferase; risk; thyroid function; thyroid hormones; China;     Show all 8 Subjects
Abstract:
... Dechloranes are a group of halogenated flame retardants with a basic bicyclo[2.2.1]heptene, including Dechlorane Plus (DP), Dechlorane 602 (Dec 602), Dechlorane 603 (Dec 603) and Dechlorane 604 (Dec 604). A few epidemiological investigations and animal experiments have shown that DP exhibited thyroid-interfering effects. In the present study, we investigated whether DP and three other dechloranes ...
DOI:
10.1016/j.jes.2021.04.030

10. TRY intron2 determined its expression in inflorescence activated by SPL9 and MADS-box genes in Arabidopsis

Author:
Wenrui Cui; Zhiwen Chen; Xiaoxia Shangguan; Taotao Li; Lingjian Wang; Xueyi Xue; Junfeng Cao
Source:
Plant science 2022 v.321 pp. 111311
ISSN:
0168-9452
Subject:
reporter genes,   etc   ; Arabidopsis; biochemistry; gene expression; genomics; introns; lint cotton; trichomes;     Show all 8 Subjects
Abstract:
... Plant trichomes are specialized epidermal cells that protect plants from insects and pathogens. In Arabidopsis, epidermal hairs decrease as internodes increase in height, with only few epidermal hairs produced on the sepals abaxial surface of the early flowers. TRIPTYCHON (TRY) is known to be a negative regulator of epidermal hair development in Arabidopsis, suppressing the formation of ectopic ep ...
DOI:
10.1016/j.plantsci.2022.111311

11. Aryl hydrocarbon reporter gene bioassay for screening polyhalogenated dibenzo-p-dioxins/furans and dioxin-like polychlorinated biphenyls in hydrochar and sewage sludge

Author:
Giulia Liberatori; Carola Mazzoli; Fabrizio Ferraro; Lucrezia Sturba; Maria Luisa Vannuccini; Davide Baroni; Peter Alexander Behnisch; Monica Puccini; Sandra Vitolo; Ilaria Corsi
Source:
Journal of hazardous materials 2022 v.428 pp. 128256
ISSN:
0304-3894
Subject:
reporter genes,   etc   ; bioassays; hydrochars; hydrothermal carbonization; polychlorinated biphenyls; sewage sludge; toxicity;     Show all 7 Subjects
Abstract:
... The suitability of the AhR reporter gene bioassays to screen the presence of polychlorinated dibenzo-p-dioxins/furans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (dl-PCBs) in sewage sludge (SL) and related hydrochar (HC) was here investigated. Samples of SL obtained from six WWTPs were processed by hydrothermal carbonization to obtain the resultant HCs and both tested with DR-CALUX® bioass ...
DOI:
10.1016/j.jhazmat.2022.128256

12. Effect of short-term temperature stress on fruit set and the expression of an auxin reporter gene and auxin synthesis genes in tomato

Author:
Hikaru Ito; Yoshinori Kanayama; Tomoki Shibuya; Seedahmed A. Mohammed; Manabu Nishiyama; Kazuhisa Kato
Source:
Scientia horticulturae 2022 v.300 pp. 111039
ISSN:
0304-4238
Subject:
reporter genes,   etc   ; auxins; cold stress; cultivars; flowering; fruit set; fruits; temperature; tomatoes;     Show all 9 Subjects
Abstract:
... Poor fruit set and growth are known to be caused by temperature stress in tomato cultivation. We focused on the effect of short-term temperature stress on each day during the five days before anthesis. The relationship between temperature stress and auxin-related factors was investigated using an experimental tomato cultivar, Micro-Tom expressing the auxin reporter DR5::GUS as a model system. Whil ...
DOI:
10.1016/j.scienta.2022.111039

13. A single promoter‐TALE system for tissue‐specific and tuneable expression of multiple genes in rice

Author:
Florence Danila; Tom Schreiber; Maria Ermakova; Lei Hua; Daniela Vlad; Shuen‐Fang Lo; Yi‐Shih Chen; Julia Lambret‐Frotte; Anna S. Hermanns; Benedikt Athmer; Susanne von Caemmerer; Su‐May Yu; Julian M. Hibberd; Alain Tissier; Robert T. Furbank; Steven Kelly; Jane A. Langdale
Source:
Plant biotechnology journal 2022 v.20 no.9 pp. 1786-1806
ISSN:
1467-7644
Subject:
reporter genes,   etc   ; biotechnology; gene expression; genetically modified organisms; rice; somaclonal variation; transgenes;     Show all 7 Subjects
Abstract:
... In biological discovery and engineering research, there is a need to spatially and/or temporally regulate transgene expression. However, the limited availability of promoter sequences that are uniquely active in specific tissue‐types and/or at specific times often precludes co‐expression of multiple transgenes in precisely controlled developmental contexts. Here, we developed a system for use in r ...
DOI:
10.1111/pbi.13864

14. Target lines for recombinase-mediated gene stacking in soybean

Author:
Li Jiang; Ruyu Li; Zhiguo Han; Xiaohui Zhao; Dong Cao; David W. Ow
Source:
Theoretical and applied genetics 2022 v.135 no.4 pp. 1163-1175
ISSN:
0040-5752
Subject:
reporter genes,   etc   ; biolistics; centromeres; cultivars; genomics; integrases; introgression; loci; plasmids; soybeans; transgenes;     Show all 11 Subjects
Abstract:
... KEY MESSAGE: Five soybean target lines with recombinase sites at suitable genomic positions were obtained and tested for site-specific gene stacking. For introgression of new transgenic traits to field cultivars, adding new DNA to an existing transgene locus would reduce the number of segregating loci to reassemble back into a breeding line. We described previously an in planta transgene stacking ...
DOI:
10.1007/s00122-021-04015-6

15. Self-immolative polyplexes for DNA delivery

Author:
Quinton E. A. Sirianni; TianDuo Wang; Aneta Borecki; Zhengyu Deng; John A. Ronald; Elizabeth R. Gillies
Source:
Biomaterials science 2022 v.10 no.10 pp. 2557-2567
ISSN:
2047-4849
Subject:
reporter genes,   etc   ; biocompatible materials; cations; cytotoxicity; depolymerization; gene expression; plasmids; polyethyleneimine; transfection;     Show all 9 Subjects
Abstract:
... Nucleic acids have immense potential for the treatment and prevention of a wide range of diseases, but delivery vehicles are needed to assist with their entry into cells. Polycations can reversibly complex with nucleic acids via ionic interactions to form polyplexes and transport them into cells, but they are still hindered by the need to balance cytotoxicity and delivery effectiveness. In this wo ...
DOI:
10.1039/d1bm01684a

16. Conserved RNA secondary structure in Cherry virus A 5′-UTR associated with translation regulation

Author:
Deya Wang; Chen Yang; Yanmei Deng; Xue Cao; Wei Xu; Zishuo Han; Qingliang Li; Yang Yang; Xuefeng Yuan
Source:
Virology journal 2022 v.19 no.1 pp. 91
ISSN:
1743-422X
Subject:
reporter genes,   etc   ; Cherry virus A; luciferase; nucleotides; phylogeny; viral genome; virology; wheat germ;     Show all 8 Subjects
Abstract:
... BACKGROUND: A variety of cis-acting RNA elements with structures in the 5′- or 3′-untranslated region (UTR) of viral genomes play key roles in viral translation. Cherry virus A (CVA) is a member of the genus Capillovirus in the family Betaflexiviridae. It has a positive single-stranded RNA genome of ~ 7400 nucleotides (nt). The length of the CVA 5′-UTR is ~ 100 nt; however, the function of this lo ...
DOI:
10.1186/s12985-022-01824-z

17. Millifolide A, a dimeric ether of degraded sesquiterpene lactones, inhibited the proliferation of human lung cancer cell line A549

Author:
Pan-Pan Yu; Feng Yu; Wen-Zhe Li; Si-Ming Wang; Chuan Wang; Mei Dong; Zhi-Yu Ni; Yong Li; Hiromasa Kiyota
Source:
Natural product research 2022 v.36 no.11 pp. 2875-2877
ISSN:
1478-6427
Subject:
reporter genes,   etc   ; Achillea millefolium; apoptosis; humans; lung neoplasms; neoplasm cells; research; sesquiterpenoid lactones;     Show all 8 Subjects
Abstract:
... The inhibitory effect of three degraded sesquiterpene lactones, iso-seco-tanapartholide, arteludooicinolide A and millifolide A isolated from Achillea millefolium L., on anti-human lung cancer cells was examined using MTT and reporter gene assays. Millifolide A has significant inhibitory effects on the proliferation of human lung cancer cells probably through inducing cell apoptosis. ...
DOI:
10.1080/14786419.2021.1922906

18. Additional data for the mouse TRPV6 expression atlas

Author:
Philipp Wartenberg; Femke Lux; Kai Busch; Claudia Fecher-Trost; Veit Flockerzi; Gabriela Krasteva-Christ; Ulrich Boehm; Petra Weissgerber
Source:
Data in Brief 2022 v.42 pp. 108201
ISSN:
2352-3409
Subject:
reporter genes,   etc   ; adults; gene expression; juveniles; mice; scanners; transient receptor potential vanilloid channels;     Show all 7 Subjects
Abstract:
... To identify TRPV6 expression in the whole mouse with a cellular resolution we took advantage of TRPV6-IRES-Cre knock-in mice crossed with the enhanced ROSA26-τGFP reporter line. In the resulting TRPV6-IC/eR26-τGFP animals, TRPV6-expressing cells are labeled with τGFP. Data were collected from organs prepared from fixed experimental adult and juvenile TRPV6-IC/eR26τGFP and Cre-negative eR26-τGFP co ...
DOI:
10.1016/j.dib.2022.108201

19. Transcription activation is enhanced by multivalent interactions independent of phase separation

Author:
Jorge Trojanowski; Lukas Frank; Anne Rademacher; Norbert Mücke; Pranas Grigaitis; Karsten Rippe
Source:
Molecular cell 2022 v.82 no.10 pp. 1878-1893.e10
ISSN:
1097-2765
Subject:
reporter genes,   etc   ; DNA-binding domains; chromatin; fluorescence microscopy; separation; transcription (genetics); transcriptional activation;     Show all 7 Subjects
Abstract:
... Transcription factors (TFs) consist of a DNA-binding domain and an activation domain (AD) that are frequently considered to be independent and exchangeable modules. However, recent studies report that the physicochemical properties of the AD can control TF assembly at chromatin by driving phase separation into transcriptional condensates. Here, we dissected transcription activation by comparing di ...
DOI:
10.1016/j.molcel.2022.04.017

20. LncRNA SNHG3 regulates the BMSC osteogenic differentiation in bone metastasis of breast cancer by modulating the miR-1273g-3p/BMP3 axis

Author:
Zijiu Sun; Jing Hu; Wei Ren; Yuting Fang; Kai Hu; Huomei Yu; Deyu Liao; Shiyan Liu; Lan Zhou; Tongchuan He; Yan Zhang
Source:
Biochemical and biophysical research communications 2022 v.594 pp. 117-123
ISSN:
0006-291X
Subject:
reporter genes,   etc   ; bone formation; bone marrow; breast neoplasms; exosomes; luciferase; metastasis; neoplasm cells; research;     Show all 9 Subjects
Abstract:
... Research on the role of lncRNAs in the process of bone metastasis in breast cancer (BM-BCa) has just begun at an early stage, and an increasing number of lncRNAs have been proved to play a regulatory role in the process of BM-BCa. Our study focused on the balance of osteogenic-osteoclast regulated by lncRNA-SNHG3 in bone metastasis microenvironment. SNHG3 level of clinical tissues and breast cance ...
DOI:
10.1016/j.bbrc.2021.12.075