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1. Intragenomic variation of rDNA internal transcribed spacers in the endemic Baikal sponge Lubomirskia baikalensis (Pallas, 1776) (Spongillida, Lubomirskiidae): Implications for Porifera barcoding

Author:
Itskovich, V.
Source:
Journal of Great Lakes research 2020 v.46 no.1 pp. 62-66
ISSN:
0380-1330
Subject:
ribosomal DNA,   etc   ; Porifera; concerted evolution; genetic markers; haplotypes; species identification;     Show all 6 Subjects
Abstract:
... Baikal endemic sponges are a species group for which species identification is difficult and the taxonomy is insufficiently developed. The rDNA ITS (internal transcribed spacers) are practical molecular markers for sponges. However, intragenomic polymorphism of the ITS spacers has recently been detected in many organisms including marine and freshwater sponges. We for the first time demonstrate in ...
DOI:
10.1016/j.jglr.2019.10.009

2. Molecular phylogeny of Eupodidae reveals that the family Cocceupodidae (Actinotrichida; Eupodoidea) and its genus Filieupodes are valid taxa

Author:
Szudarek-Trepto, Natalia; Kaźmierski, Andrzej; Dabert, Mirosława; Dabert, Jacek
Source:
Experimental & applied acarology 2020 v.80 no.1 pp. 43-57
ISSN:
0168-8162
Subject:
ribosomal DNA,   etc   ; Eupodidae; acarology; mites; mitochondria; monophyly; new genus;     Show all 7 Subjects
Abstract:
... The family Eupodidae is one of eight families belonging to the superfamily Eupodoidea. Although the Eupodidae are very common mites, they are still poorly investigated and the systematic parameters of the family are vague with unclear intrafamilial relationships between its genera. According to the hypothesis of Jesionowska (Genus 21(4):637–658, 2010), the eupodid genera Cocceupodes, Linopodes and ...
DOI:
10.1007/s10493-019-00455-4

3. An appraisal of the genus Pyropia (Bangiales, Rhodophyta) from southern Africa based on a multi-gene phylogeny, morphology and ecology, including the description of Pyropia meridionalis sp. nov.

Author:
Reddy, Maggie M.; De Clerck, Olivier; Leliaert, Frederik; Anderson, Robert J.; Bolton, John J.
Source:
South African journal of botany 2020 v.131 pp. 18-32
ISSN:
0254-6299
Subject:
ribosomal DNA,   etc   ; Pyropia; biodiversity; coasts; geographical distribution; loci; new species; phylogeny; Namibia; North America; South Africa;     Show all 11 Subjects
Abstract:
... Since its reinstatement, the diversity of the genus Pyropia has never been fully assessed in the Benguela Marine Province in southern Africa (South Africa and Namibia), although it has been included in previous, more general biodiversity assessments. The aim of the present study was to revisit the diversity and identity of species of Pyropia from the region, using an integrative taxonomic approach ...
DOI:
10.1016/j.sajb.2019.12.027

4. Assessment of species boundaries of the Moringa ovalifolia in Namibia using nuclear its DNA sequence data

Author:
Hausiku, M.K.; Kwembeya, E.G.; Chimwamurombe, P.M.; Mbangu, A.
Source:
South African journal of botany 2020 v.131 pp. 335-341
ISSN:
0254-6299
Subject:
ribosomal DNA,   etc   ; Moringa; dietary supplements; ecosystems; internal transcribed spacers; monophyly; shrubs; subtropics; trees; Namibia;     Show all 10 Subjects
Abstract:
... The genus Moringa is a phenotypically varied group of trees and shrubs, widely distributed in the tropical and subtropical regions, whose species are mostly sought after for nutritional, nutraceutical and industrial use. The Namibian Moringa ovalifolia consists of phenotypically different populations scattered across the country and occurring in different ecological systems. In the present study, ...
DOI:
10.1016/j.sajb.2020.03.002

5. Allocreadium khankaiensis sp.nov. and Allocreadium hemibarbi Roitman, 1963 (Trematoda: Allocreadiidae) from the Russian Far East: Morphological, molecular, and phylogenetic studies

Author:
Vainutis, Konstantin S.
Source:
Parasitology international 2020 v.76 pp. 102102
ISSN:
1383-5769
Subject:
ribosomal DNA,   etc   ; Allocreadium; Cyprinidae; genes; genetic analysis; morphometry; phylogeny; ribosomal RNA; testes; watersheds; East Asia; Russia;     Show all 12 Subjects
Abstract:
... Three species of the genus Allocreadium were collected from cyprinid fish in the territory of the Russian Far East: Allocreadium khankaiensis sp.nov; Allocreadium hemibarbi Roitman, 1963, and Allocreadium sp. 1. It is established that, according to morphometric values, the species A. khankaiensis sp.nov. has high morphological similarity with Allocreadium elongatum Achmerov, 1960; Allocreadium ery ...
DOI:
10.1016/j.parint.2020.102102

6. Morphological description of Opalina obtrigonoidea Metcalf, 1923 (Heterokonta, Opalinea) from Duttaphrynus melanostictus and evaluation of the ITS region as a suitable genetic marker for inter-species identification in Opalina

Author:
Zhao, Weishan; Hu, Guangran; Ponce-Gordo, Francisco; Zou, Hong; Li, Wenxiang; Wu, Shangong; Li, Ming; Wang, Guitang
Source:
Parasitology international 2020 v.76 pp. 102103
ISSN:
1383-5769
Subject:
ribosomal DNA,   etc   ; Opalinea; genes; genetic markers; internal transcribed spacers; rectum; redescriptions; sequence analysis; toads;     Show all 9 Subjects
Abstract:
... The redescription of Opalina obtrigonoidea Metcalf, 1923, collected from the rectum of the toads Duttaphrynus melanostictus, is presented in this paper based on detailed morphological information and molecular data. Our results revealed that O. obtrigonoidea varies greatly in body dimensions. Its morphological characteristics allow its differentiation from Opalina undulata. Surprisingly, we sequen ...
DOI:
10.1016/j.parint.2020.102103

7. Phylogenetic analysis of phenotypic characters of Tunicata supports basal Appendicularia and monophyletic Ascidiacea

Author:
Braun, Katrin; Leubner, Fanny; Stach, Thomas
Source:
Cladistics 2020 v.36 no.3 pp. 259-300
ISSN:
0748-3007
Subject:
ribosomal DNA,   etc   ; Ascidiacea; data collection; life history; monophyly; nucleotide sequences; paraphyly; phenotype; plankton;     Show all 9 Subjects
Abstract:
... With approximately 3000 marine species, Tunicata represents the most disparate subtaxon of Chordata. Molecular phylogenetic studies support Tunicata as sister taxon to Craniota, rendering it pivotal to understanding craniate evolution. Although successively more molecular data have become available to resolve internal tunicate phylogenetic relationships, phenotypic data have not been utilized cons ...
DOI:
10.1111/cla.12405

8. Cohesin Causes Replicative DNA Damage by Trapping DNA Topological Stress

Author:
Minchell, Nicola Elizabeth; Keszthelyi, Andrea; Baxter, Jonathan
Source:
Molecular cell 2020 v.78 no.4 pp. 739-751.e8
ISSN:
1097-2765
Subject:
ribosomal DNA,   etc   ; DNA damage; DNA replication; Saccharomyces cerevisiae; centromeres; genome; inheritance (genetics);     Show all 7 Subjects
Abstract:
... DNA topological stress inhibits DNA replication fork (RF) progression and contributes to DNA replication stress. In Saccharomyces cerevisiae, we demonstrate that centromeric DNA and the rDNA array are especially vulnerable to DNA topological stress during replication. The activity of the SMC complexes cohesin and condensin are linked to both the generation and repair of DNA topological-stress-link ...
DOI:
10.1016/j.molcel.2020.03.013

9. Ceratomyxa mennani n. sp. (Myxosporea: Bivalvulida) parasitizing the gallbladder of the dusky grouper Epinephelus marginatus (Serranidae) from Tunisian waters

Author:
Bouderbala, Khouloud; Rangel, Luis F.; Santos, Maria J.; Bahri, Sihem
Source:
Parasitology research 2020 v.119 no.5 pp. 1515-1522
ISSN:
0932-0113
Subject:
ribosomal DNA,   etc   ; Ceratomyxa; Epinephelus marginatus; autumn; fish; gall bladder; nucleotide sequences; parasitism; phylogeny; winter; Tunisia;     Show all 11 Subjects
Abstract:
... Ceratomyxa mennani n. sp. is a new coelozoic Ceratomyxa species found in the gallbladder of Epinephelus marginatus from the Gulf of Tunis, Tunisia. Mature plasmodia were disporic, ovoid in shape measuring 9–12 μm in width and 11–14 μm in length. Mature myxospores were slightly crescent-shaped with almost straight posterior margin, measuring 5.8 ± 0.2 (5.4–6.1) μm in length and 12.7 ± 0.3 (11.9–13. ...
DOI:
10.1007/s00436-020-06649-9

10. Characterization of Myxidium spinibarba sp. nov. (Cnidaria, Myxosporea, Myxidiidae) from Spinibarbus sinensis (Bleeker, 1871) in Chongqing China

Author:
Chen, Wei; Yang, Chengzhong; Zhao, Yuanjun
Source:
Parasitology research 2020 v.119 no.5 pp. 1485-1491
ISSN:
0932-0113
Subject:
ribosomal DNA,   etc   ; Myxidium; Spinibarbus; gall bladder; new species; nucleotide sequences; phylogeny; rivers; China;     Show all 9 Subjects
Abstract:
... In the present study, we described a new species of Myxidium Bütschli, 1882, obtained from the gallbladder of Spinibarbus sinensis (Bleeker, 1871) from the Jialing River in Chongqing, China. Myxidium spinibarba sp. nov. was identified based on morphological and SSU rDNA sequence data. The mature myxospores were fusiform in valvular view and ovoid in sutural view, with somewhat protrusive poles and ...
DOI:
10.1007/s00436-020-06644-0

11. Genome size and cytomolecular diversification in two species of the South African endemic genus Tulbaghia L. (Allioideae, Amaryllidaceae)

Author:
Báez, Mariana; Souza, Gustavo; Guerra, Marcelo
Source:
South African journal of botany 2020 v.130 pp. 407-413
ISSN:
0254-6299
Subject:
ribosomal DNA,   etc   ; Tulbaghia; chromosome morphology; chromosome number; evolution; genome; genomics; heterochromatin; indigenous species; karyotyping; telomeres;     Show all 11 Subjects
Abstract:
... Tulbaghia L. is a South African genus of Allioideae, Amaryllidaceae, which includes circa 25 species with similar karyotypes, all of them with 2n = 12 large chromosomes, predominantly metacentric to submetacentric. This karyotype stability contrasts with the high variability in chromosome number and morphology reported for its South American sister group, the tribe Gilliesieae. Aiming to understan ...
DOI:
10.1016/j.sajb.2020.01.024

12. Intra-genomic variability and pseudogenes in ribosomal ITS regions of Paraplagusia blochii (Pleuronectiformes: Cynoglossidae)

Author:
Gong, Li; Kong, Xiaoyu; Luo, Hairong; Chen, Shixi; Shi, Wei; Yang, Min
ISSN:
1570-7555
Subject:
ribosomal DNA,   etc   ; Gibbs free energy; Paraplagusia blochii; cluster analysis; evolution; fish; internal transcribed spacers; pseudogenes;     Show all 8 Subjects
Abstract:
... Eukaryotic nuclear ribosomal DNA (rDNA) typically evolves in a concerted manner, in which hundreds of rDNA sequences within species show little or no variations, whereas the sequences of different species diverge. There are few studies of the internal transcribed spacer regions (ITS1-5.8S-ITS2) in teleostean fishes and only one report on flatfishes. Here, we reported the discovery of two types of ...
DOI:
10.1163/15707563-20191141

13. Genomic and geochemical identification of the long-chain alkenone producers in the estuarine Lake Takahoko, Japan: Implications for temperature reconstructions

Author:
Kajita, Hiroto; Nakamura, Hideto; Ohkouchi, Naohiko; Harada, Naomi; Sato, Miyako; Tokioka, Shun; Kawahata, Hodaka
Source:
Organic geochemistry 2020 v.142 pp. 103980
ISSN:
0146-6380
Subject:
ribosomal DNA,   etc   ; Isochrysis galbana; estuaries; genomics; lakes; phylotype; sediments; summer; water temperature; Japan; United States;     Show all 11 Subjects
Abstract:
... Identifying the lacustrine haptophyte species that produce long-chain alkenones (LCAs) is essential for using alkenone unsaturation ratios to create lake water temperature reconstructions. We discovered LCAs in the brackish Lake Takahoko in northern Japan. The identity of LCA-producing species was investigated using 18S ribosomal DNA (rDNA) and organic geochemical analysis. Two distinct genetic gr ...
DOI:
10.1016/j.orggeochem.2020.103980

14. Molecular characterization of Raillietina isolates from the gastrointestinal tract of free range chickens (Gallus Gallus domesticus) from the southern region of Zimbabwe using the 18S rDNA gene

Author:
Makwanise, T.; Dube, S.; Sibula, M.S.
Source:
Veterinary Parasitology: Regional Studies and Reports 2020 v.20 pp. 100389
ISSN:
2405-9390
Subject:
ribosomal DNA,   etc   ; Raillietina; chickens; genes; intestines; microscopy; parasitoses; phylogeny; polymerase chain reaction; poultry production; tapeworms; Zimbabwe;     Show all 12 Subjects
Abstract:
... Parasitic diseases are among the major constraints of poultry production. The common internal parasitic infections occurring in poultry include cestodes and other gastrointestinal helminths. The aim of this study was to characterize Raillietina spp. from the gastrointestinal tract of free-range chickens using 18S rDNA genes. This was achieved through the DNA extraction from tapeworms isolated from ...
DOI:
10.1016/j.vprsr.2020.100389

15. Molecular phylogenetics of deep-sea amphipods (Eurythenes) reveal a new undescribed species at the Porcupine Abyssal Plain, North East Atlantic Ocean

Author:
Horton, Tammy; Cooper, Harry; Vlierboom, Rianna; Thurston, Michael; Hauton, Chris; Young, C. Robert
Source:
Progress in oceanography 2020 v.183 pp. 102292
ISSN:
0079-6611
Subject:
ribosomal DNA,   etc   ; Amphipoda; genes; genetic barcoding; mitochondria; phylogeny; taxonomy; Atlantic Ocean;     Show all 8 Subjects
Abstract:
... The genus Eurythenes S. I. Smith in Scudder, 1882, has been the focus of integrated molecular and morphological taxonomy studies in recent years, resulting in the number of species in the genus increasing from three to eight. Samples of Eurythenes spp. collected using free-fall baited traps from the Porcupine Abyssal Plain (PAP), North East Atlantic Ocean, at 4850 m were examined in this study to ...
DOI:
10.1016/j.pocean.2020.102292

16. New species and new genus of Pseudohaploporinae (Digenea): Pseudohaploporus pusitestis sp. n. and Parahaploporus elegantus n. g., sp. n. (Digenea: Pseudohaploporinae) from Vietnamese mullet fish

Author:
Atopkin, D.M.; Besprozvannykh, V.V.; Ha, D.N.; Nguyen, V.H.; Nguyen, V.T.
Source:
Parasitology international 2020 v.75 pp. 102023
ISSN:
1383-5769
Subject:
ribosomal DNA,   etc   ; Trematoda; eggs; hermaphroditism; intestines; monophyly; mullet; new genus; new species; sphincters; testes;     Show all 11 Subjects
Abstract:
... Two new species of Pseudohaploporinae, Pseudohaploporus pusitestis sp. n. and Parahaploporus elegantus n. g., sp. n., are described from intestines of the Vietnamese mullet fish Moolgarda seheli and Osteomugil cunnesius, respectively. Pseudohaploporus pusitestis sp. n. differs from two known Pseudohaploporus species, P. vietnamensis and P. planiliza, by the absence of a diverticulate hermaphroditi ...
DOI:
10.1016/j.parint.2019.102023

17. Novel myxosporean species parasitizing an economically important fish from the Amazon basin

Author:
Capodifoglio, Kassia R. H.; Adriano, Edson A.; Naldoni, Juliana; Meira, Caroline M.; da Silva, Marcia R. M.; Maia, Antonio A. M.
Source:
Parasitology research 2020 v.119 no.4 pp. 1209-1220
ISSN:
0932-0113
Subject:
ribosomal DNA,   etc   ; Henneguya; Myxobolus; Piaractus brachypomus; basins; cecum; deformation; epithelium; fish; parasitism; phylogeny; rivers; Brazil;     Show all 13 Subjects
Abstract:
... This paper provides morphological and phylogenetic analyses of two new myxobolid species found infecting Piaractus brachypomus from the Amazon basin. The fish were caught in the Tapajós River, in the municipality of Santarém, in the state of Pará, Brazil. The plasmodial development of Henneguya brachypomus n. sp. occurred in the gill lamellae while Myxobolus pirapitingae n. sp. developed in the py ...
DOI:
10.1007/s00436-020-06641-3

18. Phylogenetic characterisation of seven Unicapsula spp. (Myxozoa: Myxosporea: Multivalvulida) from commercial fish in southern China and Japan

Author:
Li, Ying-Chun; Tamemasa, Souhei; Zhang, Jin-Yong; Sato, Hiroshi
Source:
Parasitology 2020 v.147 no.4 pp. 448-464
ISSN:
1469-8161
Subject:
ribosomal DNA,   etc   ; Multivalvulida; gills; marine fish; monophyly; new species; nucleotide sequences; protozoal infections; China; Japan;     Show all 10 Subjects
Abstract:
... The myxozoan genus Unicapsula Davis, 1924 (Myxosporea: Multivalvulida: Trilosporidae) is characterized as having one functional polar capsule (PC) and two rudimentary PCs in a three-valved myxospore. The plasmodia of Unicapsula spp. grow either in the myofibres or in the gills, oesophageal walls and urinary organs of marine fish. Few studies have investigated the taxonomy of Unicapsula spp. includ ...
DOI:
10.1017/S0031182019001793

19. Sarcocystis spp. diversity in the roe deer (Capreolus capreolus) from Lithuania and Spain

Author:
Rudaitytė-Lukošienė, E.; Delgado de las Cuevas, G. E.; Prakas, P.; Calero-Bernal, R.; Martínez-González, M.; Strazdaitė-Žielienė, Ž.; Servienė, E.; Habela, M. A.; Butkauskas, D.
Source:
Parasitology research 2020 v.119 no.4 pp. 1363-1370
ISSN:
0932-0113
Subject:
ribosomal DNA,   etc   ; Capreolus capreolus; Sarcocystis; animals; diaphragm; genes; genetic variation; intermediate hosts; sarcocysts; tongue; Lithuania; Spain;     Show all 12 Subjects
Abstract:
... The roe deer (Capreolus capreolus) has been identified as an intermediate host for six known Sarcocystis species, S. capreolicanis, S. entzerothi, S. gracilis, S. linearis, S. oviformis, and S. silva. In this study, we identified Sarcocystis species in the diaphragm and tongue muscles from the Lithuanian and Spanish roe deer, respectively, on the basis of a microscopic examination and DNA analysis ...
DOI:
10.1007/s00436-020-06603-9

20. Chloroplast and nuclear ribosomal cistron phylogenomics in a group of closely related sections in Salvia subg. Calosphace

Author:
Olvera-Mendoza, Edgar I.; Godden, Grant T.; Montero-Castro, Juan Carlos; Porter, J. Mark; Lara-Cabrera, Sabina I.
Source:
Revista brasileira de botânica 2020 v.43 no.1 pp. 177-191
ISSN:
0100-8404
Subject:
ribosomal DNA,   etc   ; Salvia miltiorrhiza; chloroplast genome; chloroplasts; genes; internal transcribed spacers; loci; monophyly; sage; statistical analysis;     Show all 10 Subjects
Abstract:
... Sage phylogenies have not resolved many shallow level clades within problematic Salvia subgenus Calosphace, hindered by the difficulty in finding sufficiently variable loci. We assembled fifteen chloroplast genomes and nuclear ribosomal cistrons from non-target reads of a nuclear hybrid enrichment project to assess the phylogenetic utility for a closely related group of mainly Mexican sections. Ch ...
DOI:
10.1007/s40415-019-00572-9