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1. Direct triplex loop-mediated isothermal amplification assay for the point-of-care molecular detection of Salmonella genus, subspecies I, and serovar Typhimurium

Author:
Kim, Mi-Ju; Kim, Hyun-Joong; Kim, Hae-Yeong
Source:
Food control 2021 v.120 pp. 107504
ISSN:
0956-7135
Subject:
safety testing,   etc   ; DNA; Salmonella; annealing; assays; chicken meat; cross reaction; detection limit; diagnostic techniques; extraction; food contamination; food matrix; food pathogens; genes; loop-mediated isothermal amplification; microbial detection; models; peptidase K; point-of-care systems; sampling; serotypes;     Show all 21 Subjects
Abstract:
... In this study, a novel triplex loop-mediated isothermal amplification (LAMP) assay was developed and applied to a model of representative foodborne pathogen, Salmonella, to investigate the performance of the assay with high rapidity, specificity and sensitivity, and its ability to detect a pathogen in contaminated food. Three sets of LAMP primers were designed for target genes in Salmonella genus, ...
DOI:
10.1016/j.foodcont.2020.107504

2. Phylogenetic analysis of infectious bronchitis virus circulating in southern China in 2016–2017 and evaluation of an attenuated strain as a vaccine candidate

Author:
Chen, Libin; Xiang, Bin; Hong, Yanfen; Li, Qian; Du, Haoyun; Lin, Qiuyan; Liao, Ming; Ren, Tao; Xu, Chenggang
Source:
Archives of virology 2021 v.166 no.1 pp. 73-81
ISSN:
0304-8608
Subject:
safety testing,   etc   ; Infectious bronchitis virus; chicken eggs; chickens; farms; genotype; infection; infectious bronchitis; live vaccines; phylogeny; poultry industry; protective effect; respiratory tract diseases; signs and symptoms (animals and humans); specific pathogen-free animals; strains; viral shedding; virology; China;     Show all 19 Subjects
Abstract:
... Avian infectious bronchitis (IB) is a highly contagious viral respiratory disease, caused by infectious bronchitis virus (IBV), that poses an important economic threat to the poultry industry. In recent years, genotypes GI-7, GI-13, and GI-19 have been the most prevalent IBV strains in China. However, in this study, we found that most IBV strains from southern China in 2016–2017 belonged to genoty ...
DOI:
10.1007/s00705-020-04851-9

3. Evaluation of the monocyte activation test for the safety testing of meningococcal B vaccine Bexsero: A collaborative study

Author:
Studholme, Lucy; Sutherland, Janet; Desai, Trusha; Hockley, Jason; Care, Rory; Nordgren, Ida Karin; Vipond, Caroline
Source:
Vaccine 2019 v.37 no.29 pp. 3761-3769
ISSN:
0264-410X
Subject:
safety testing,   etc   ; data collection; geometry; monocytes; rabbits; vaccines;     Show all 6 Subjects
Abstract:
... The aim of this collaborative study was to evaluate the robustness of the monocyte activation test (MAT) for quantifying the pyrogenic content in the outer membrane vesicle (OMV)-containing vaccine Bexsero: the first meningococcal B vaccine to be licenced. We analysed datasets from 9 laboratories covering 15 test systems for 3 batches of Bexsero with higher, equivalent and lower activity relative ...
DOI:
10.1016/j.vaccine.2018.05.073

4. Flexible, stable and sensitive surface-enhanced Raman scattering of graphite/titanium-cotton substrate for conformal rapid food safety detection

Author:
Xu, Jiangtao; Li, Xiaoting; Wang, Yuxiang; Hua, Tao; Guo, Ronghui; Miao, Dagang; Jiang, Shouxiang
Source:
Cellulose 2020 v.27 no.2 pp. 941-954
ISSN:
0969-0239
Subject:
safety testing,   etc   ; Raman spectroscopy; ambient temperature; cotton; cotton fabric; detection limit; food safety; graphene; rapid methods; semiconductors; titanium;     Show all 11 Subjects
Abstract:
... Fabricating flexible, stable and highly reproducible surface-enhanced Raman scattering (SERS) active substrates is very important in furthering the development of practical SERS sensors. Nevertheless, the fabrication of such SERS substrates is rather challenging in processing conditions for both noble metal and semiconductor substrates. In this study, highly sensitive SERS detection is achieved by ...
DOI:
10.1007/s10570-019-02836-9

5. High-performance detection of Mycobacterium bovis in milk using digital LAMP

Author:
Tao, Yi; Yun, Juanli; Wang, Jian; Xu, Peng; Li, Caiming; Liu, Hongtao; Lan, Ying; Pan, Jianzhang; Du, Wenbin
Source:
Food chemistry 2020 v.327 pp. 126945
ISSN:
0308-8146
Subject:
safety testing,   etc   ; DNA; Mycobacterium bovis; automation; detection limit; emulsifying; food safety; humans; loop-mediated isothermal amplification; microbial detection; milk; pathogens; quantitative polymerase chain reaction; tuberculosis; zoonoses;     Show all 15 Subjects
Abstract:
... This paper described a high-performance molecular test for the detection of Mycobacterium bovis (M. bovis) based on digital loop-mediated isothermal amplification (dLAMP). M. bovis is a persistent pathogen that causes zoonotic tuberculosis and can infect both animals and human beings. The detection of M. bovis in milk samples is critical for effective control and prevention of zoonotic diseases bu ...
DOI:
10.1016/j.foodchem.2020.126945

6. Propagation, Inactivation, and Safety Testing of SARS-CoV-2

Author:
Jureka, Alexander S.; Silvas, Jesus A.; Basler, Christopher F.
Source:
Viruses 2020 v.12 no.6
ISSN:
1999-4915
Subject:
safety testing,   etc   ; COVID-19 infection; DNA; RNA; Severe acute respiratory syndrome coronavirus 2; agarose; biosafety; cell lines; cellulose; formalin; heat; lactones; pandemic; pathogenicity; viruses; China;     Show all 16 Subjects
Abstract:
... In late 2019, a novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, the capital of the Chinese province Hubei. Since then, SARS-CoV-2 has been responsible for a worldwide pandemic resulting in over 4 million infections and over 250,000 deaths. The pandemic has instigated widespread research related to SARS-CoV-2 and the disease that it causes, COVID-19 ...
DOI:
10.3390/v12060622

7. Severity assessment in pigs after partial liver resection: evaluation of a score sheet

Author:
Hagemeister, Kerstin; Ernst, Lisa; Kadaba Srinivasan, Pramod; Tanaka, Hirokazu; Fukushima, Kenji; Tolba, René
Source:
Laboratory animals 2020 v.54 no.3 pp. 251-260
ISSN:
1758-1117
Subject:
safety testing,   etc   ; behavior; biomedical research; blood; distress; estimation; fibrin; hands; laboratory animals; laparotomy; liver; miniature swine; models; pain; resection; sealants; swine; temperature;     Show all 18 Subjects
Abstract:
... Severity assessment in biomedical research is required by the European authorities. Therefore, a variety of score sheets are available. The first score sheets were designed and introduced by Morton and Griffith (M&G) in 1985, to assess pain and distress in animals. Score sheets are an important part of the 3R principles to evaluate the degree of severity in different studies. Here, we used a modif ...
DOI:
10.1177/0023677219871585

8. Testing for thyroid hormone disruptors, a review of non-mammalian in vivo models

Author:
Couderq, Stephan; Leemans, Michelle; Fini, Jean-Baptiste
Source:
Molecular and cellular endocrinology 2020 v.508 pp. 110779
ISSN:
0303-7207
Subject:
safety testing,   etc   ; Danio rerio; Xenopus laevis; animal models; animal use replacement; chemical safety; frogs; histopathology; ligands; mammals; neurodevelopment; receptors; screening; thyroid hormones;     Show all 14 Subjects
Abstract:
... Thyroid hormones (THs) play critical roles in profound changes in many vertebrates, notably in mammalian neurodevelopment, although the precise molecular mechanisms of these fundamental biological processes are still being unravelled. Environmental and health concerns prompted the development of chemical safety testing and, in the context of endocrine disruption, identification of thyroid hormone ...
DOI:
10.1016/j.mce.2020.110779

9. A strategy for test article selection and phytochemical characterization of Echinacea purpurea extract for safety testing

Author:
Waidyanatha, Suramya; Pierfelice, Jessica; Cristy, Tim; Mutlu, Esra; Burback, Brian; Rider, Cynthia V.; Ryan, Kristen
Source:
Food and chemical toxicology 2020 v.137 pp. 111125
ISSN:
0278-6915
Subject:
safety testing,   etc   ; DNA; Echinacea purpurea; animal models; caftaric acid; chemical analysis; chemical constituents of plants; chemometrics; chlorogenic acid; dietary supplements; medicinal properties; phytochemicals; root extracts;     Show all 13 Subjects
Abstract:
... Botanical dietary supplements (BDS) are used around the world for many purported therapeutic properties. The selection of an authentic product and it's phytochemical characterization is critical to generate robust safety data. Because botanicals are complex mixtures with variable quality, identification of a representative product for testing has been challenging. Echinacea is used for its purport ...
DOI:
10.1016/j.fct.2020.111125
PubMed:
31931071
PubMed Central:
PMC7079738

10. Integrated immunochromatographic assay for qualitative and quantitative detection of clenbuterol

Author:
Chen, Yuan; Huang, Zhen; Hu, Song; Zhang, Ganggang; Peng, Juan; Xia, Jun; Lai, Weihua
Source:
Analytical biochemistry 2019 v.577 pp. 45-51
ISSN:
0003-2697
Subject:
safety testing,   etc   ; antibodies; antigens; clenbuterol; detection limit; fluorescence; gold; immunoaffinity chromatography; monitoring; nanogold; nanoparticles; screening;     Show all 12 Subjects
Abstract:
... In this study, colloidal gold (CG) and time-resolved fluorescent nanobead (TRFN) probes were used to establish an integrated immunochromatographic assay (ICA) to qualitatively and quantitatively detect clenbuterol (CLE). The best experimental conditions for the two probes in separate ICAs were obtained by optimizing the antibody labeling concentration, the amount of antigen, and the concentration ...
DOI:
10.1016/j.ab.2019.04.013

11. Development and validation of a monocyte activation test for the control/safety testing of an OMV-based meningococcal B vaccine

Author:
Vipond, Caroline; Sutherland, Janet; Nordgren, Karin; Kemp, George; Heath, Alan; Care, Rory; Studholme, Lucy
Source:
Vaccine 2019 v.37 no.29 pp. 3747-3753
ISSN:
0264-410X
Subject:
safety testing,   etc   ; clinical trials; endotoxins; intravenous injection; lipoproteins; medicine; monocytes; patients; porins; rabbits; vaccines; United Kingdom;     Show all 12 Subjects
Abstract:
... It is imperative to ensure biological products are free of contaminating pyrogenic material prior to administration to patients. Historically the rabbit pyrogen test (RPT) was used to screen for such contamination in medicines for intravenous delivery. This test was adapted for use to screen vaccines. However, some, including meningococcal vaccines containing outer membrane vesicles, are intrinsic ...
DOI:
10.1016/j.vaccine.2018.06.038

12. Physiological impacts of time in holding ponds, biomedical bleeding, and recovery on the Atlantic horseshoe crab, Limulus polyphemus

Author:
Hamilton, Kristin Linesch; Burnett, Louis; Burnett, Karen; Kalisperis, Rachel E.G.; Fowler, Amy E.
Source:
Comparative biochemistry and physiology 2019
ISSN:
1095-6433
Subject:
safety testing,   etc   ; Limulus polyphemus; analysis of variance; body weight; coasts; crabs; females; hemocyanin; hemocytes; hemorrhage; mortality; ponds; synergism; South Carolina;     Show all 14 Subjects
Abstract:
... Atlantic horseshoe crabs, Limulus polyphemus (HSC), are commercially harvested along the eastern U.S. coast and bled for hemolymph used in pharmaceutical safety testing. In South Carolina, some HSCs are held in outdoor ponds before transport to facilities where they are bled and then released to the wild. This study tested whether the time HSCs are held before bleeding, bleeding itself, or the dur ...
DOI:
10.1016/j.cbpa.2019.110554

13. Performance and microbiological safety testing after multiple use cycles and hydrogen peroxide sterilization of a 5‐mm vessel‐sealing device

Author:
Valenzano, D.; Hayes, G.; Gludish, D.; Weese, S.
Source:
Veterinary surgery 2019 v.48 no.5 pp. 885-889
ISSN:
0161-3499
Subject:
safety testing,   etc   ; bacterial culture; carotid arteries; cleaning; dogs; endoscopes; hydrogen peroxide; microbiological quality; ovariectomy; patients; risk; sterilizing; swine;     Show all 13 Subjects
Abstract:
... OBJECTIVE: To evaluate the functional and microbiological safety of a single‐use laparoscopic vessel‐sealing device in a multiuse setting. STUDY DESIGN: Ex vivo study. SAMPLE POPULATION: Twelve 5‐mm LigaSure Maryland jaw devices. METHODS: Handsets underwent repeated test cycles until failure. The handset packaging was opened, and handsets were agitated in phosphate‐buffered saline (PBS). The PBS w ...
DOI:
10.1111/vsu.13191

14. Applications of cyclic peptide nanotubes (cPNTs)

Author:
Hsieh, Wei-Hsien; Liaw, Jiahorng
Source:
Yàowù shípǐn fēnxī 2019 v.27 no.1 pp. 32-47
ISSN:
1021-9498
Subject:
safety testing,   etc   ; amino acid sequences; antibiotics; drugs; electronic equipment; enantiomers; gene transfer; medical equipment; nanotubes; permeability; surface area;     Show all 11 Subjects
Abstract:
... Self-assembled cyclic peptide nanotubes (cPNTs) have recently drawn particular attention as one of the most intriguing nanostructures in the field of nanotechnology. Given their unique features including high surface area, increased drug loading, environmental stability, enhanced permeation, and modifiable drug release, these hollow tubular structures can be constructed with cyclic di-, tri-, tetr ...
DOI:
10.1016/j.jfda.2018.09.004

15. Potassium iodide enhances the photobactericidal effect of methylene blue on Enterococcus faecalis as planktonic cells and as biofilm infection in teeth

Author:
Yuan, Lintian; Lyu, Peijun; Huang, Ying-Ying; Du, Ning; Qi, Wei; Hamblin, Michael R.; Wang, Yuguang
Source:
Journal of photochemistry and photobiology 2020 v.203 pp. 111730
ISSN:
1011-1344
Subject:
safety testing,   etc   ; Enterococcus faecalis; antibacterial properties; biofilm; biosafety; clinical trials; confocal laser scanning microscopy; disinfection; fibroblasts; fluorescence microscopy; free radicals; humans; hydrogen peroxide; hydrogen production; iodine; lighting; methylene blue; photobleaching; photochemotherapy; plankton; potassium iodide; scanning electron microscopy; staining; teeth;     Show all 24 Subjects
Abstract:
... To explore the effectiveness, biosafety, photobleaching and mechanism of antimicrobial photodynamic therapy (aPDT) using methylene blue (MB) plus potassium iodide (KI), for root canal infections.Different combinations and concentrations of MB, KI and 660 nm LED light were used against E. faecalis in planktonic and in biofilm states by colony-forming unit (CFU), confocal laser scanning microscopy ( ...
DOI:
10.1016/j.jphotobiol.2019.111730
PubMed:
31855718
PubMed Central:
PMC6947667

16. Comparison of Flame Propagation in a Tube with a Flexible/Rigid Obstacle

Author:
Li, Quan; Lu, Shouxiang; Xu, Mingjun; Ding, Yanming; Wang, Changjian
Source:
Energy & Fuels 2016 v.30 no.10 pp. 8720-8726
ISSN:
1520-5029
Subject:
safety testing,   etc   ; combustion; hydrogen production; transportation;     Show all 4 Subjects
Abstract:
... The safety issues in hydrogen production, storage, transportation, and utilization have caused a high level of concern. Flame acceleration stimulated by the rigid obstacles has been much more addressed, while little attention has been paid to the cases with the flexible obstacle, which exists popularly in explosion scenarios. In this paper, a series of experiments was conducted to study the effect ...
DOI:
10.1021/acs.energyfuels.6b01594

17. Vaccine safety testing using magnetic resonance imaging in suckling pigs

Author:
Bernau, Maren; Liesner, Bernd Große; Schwanitz, Sebastian; Kraus, Ann-Sophie; Falkenau, Almuth; Leipig-Rudolph, Miriam; Hermanns, Walter; Scholz, Armin Manfred
Source:
Vaccine 2018 v.36 no.13 pp. 1789-1795
ISSN:
0264-410X
Subject:
safety testing,   etc   ; animal use reduction; magnetic resonance imaging; piglets; sodium chloride; suckling; vaccination; vaccines;     Show all 8 Subjects
Abstract:
... Safety testing is one major part of the licensing procedure for veterinary vaccines and demands a large number of animals. Magnetic resonance imaging (MRI) was tested as an alternative, which may lead to a reduction in numbers of animals required for safety testing, and, correspondingly to a detailed description of the three-dimensional extent of the local tissue reaction repetitively in live pigs ...
DOI:
10.1016/j.vaccine.2018.02.025

18. Engineered nanomaterials: From their properties and applications, to their toxicity towards marine bivalves in a changing environment

Author:
De Marchi, Lucia; Coppola, Francesca; Soares, Amadeu M.V.M.; Pretti, Carlo; Monserrat, José M.; Torre, Camilla della; Freitas, Rosa
Source:
Environmental research 2019
ISSN:
0013-9351
Subject:
safety testing,   etc   ; Bivalvia; agricultural products; aquatic ecosystems; aquatic environment; benthic organisms; climate change; ecotoxicology; environmental technology; estuaries; nanomaterials; particulates; risk assessment; toxicity;     Show all 14 Subjects
Abstract:
... As a consequence of their unique characteristics, the use of Engineered Nanomaterials (ENMs) is rapidly increasing in industrial, agricultural products, as well as in environmental technology. However, this fast expansion and use make likely their release into the environment with particular concerns for the aquatic ecosystems, which tend to be the ultimate sink for this type of contaminants. Cons ...
DOI:
10.1016/j.envres.2019.108683

19. Absorption of wireless radiation in the child versus adult brain and eye from cell phone conversation or virtual reality

Author:
Fernández, C.; de Salles, A.A.; Sears, M.E.; Morris, R.D.; Davis, D.L.
Source:
Environmental research 2018 v.167 pp. 694-699
ISSN:
0013-9351
Subject:
safety testing,   etc   ; absorption; adults; brain; children; compliance; ears; education; eyes; head; males; medical equipment; mobile telephones; models; temperature;     Show all 15 Subjects
Abstract:
... Children's brains are more susceptible to hazardous exposures, and are thought to absorb higher doses of radiation from cell phones in some regions of the brain. Globally the numbers and applications of wireless devices are increasing rapidly, but since 1997 safety testing has relied on a large, homogenous, adult male head phantom to simulate exposures; the “Standard Anthropomorphic Mannequin” (SA ...
DOI:
10.1016/j.envres.2018.05.013

20. Comprehensive Study of the Flow Control Strategy in a Wirelessly Charged Centrifugal Microfluidic Platform with Two Rotation Axes

Author:
Zhu, Yunzeng; Chen, Yiqi; Meng, Xiangrui; Wang, Jing; Lu, Ying; Xu, Youchun; Cheng, Jing
Source:
Analytical chemistry 2017 v.89 no.17 pp. 9315-9321
ISSN:
1520-6882
Subject:
safety testing,   etc   ; chromium; environmental monitoring; food safety; mixing; nucleic acids; proteins; spinning;     Show all 8 Subjects
Abstract:
... Centrifugal microfluidics has been widely applied in the sample-in–answer-out systems for the analyses of nucleic acids, proteins, and small molecules. However, the inherent characteristic of unidirectional fluid propulsion limits the flexibility of these fluidic chips. Providing an extra degree of freedom to allow the unconstrained and reversible pumping of liquid is an effective strategy to addr ...
DOI:
10.1021/acs.analchem.7b02080