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1. Curcumin stimulates exosome/microvesicle release in an in vitro model of intracellular lipid accumulation by increasing ceramide synthesis

Author:
García-Seisdedos, David; Babiy, Bohdan; Lerma, Milagros; Casado, María E.; Martínez-Botas, Javier; Lasunción, Miguel A.; Pastor, Óscar; Busto, Rebeca
Source:
Biochimica et biophysica acta 2020 v.1865 no.5 pp. 158638
ISSN:
1388-1981
Subject:
serine C-palmitoyltransferase,   etc   ; Curcuma longa; animal models; ceramides; curcumin; enzyme inhibitors; exosomes; fumonisin B1; hydrophobicity; mice; neuroglia; polyphenols; rhizomes; secretion; sphingosine N-acyltransferase;     Show all 15 Subjects
Abstract:
... Curcumin, a hydrophobic polyphenol found in the rhizome of Curcuma longa, has been shown to reduce intracellular lipid accumulation in mouse models of lysosomal storage diseases such as Niemann-Pick type C. Exosomes are small extracellular vesicles secreted by cells in response to changes in intracellular ceramide composition. Curcumin can induce exosome/microvesicle release in cellular models of ...
DOI:
10.1016/j.bbalip.2020.158638

2. The ORMs interact with transmembrane domain 1 of Lcb1 and regulate serine palmitoyltransferase oligomerization, activity and localization

Author:
Han, Gongshe; Gupta, Sita D.; Gable, Kenneth; Bacikova, Dagmar; Sengupta, Nivedita; Somashekarappa, Niranjanakumari; Proia, Richard L.; Harmon, Jeffrey M.; Dunn, Teresa M.
Source:
Biochimica et biophysica acta 2019 v.1864 no.3 pp. 245-259
ISSN:
1388-1981
Subject:
serine C-palmitoyltransferase,   etc   ; image analysis; mammals; mutants; oligomerization; phosphorylation; precipitin tests; proteins; topology; yeasts;     Show all 10 Subjects
Abstract:
... Serine palmitoyltransferase (SPT), an endoplasmic reticulum-localized membrane enzymecomposed of acatalytic LCB1/LCB2 heterodimer and a small activating subunit (Tsc3 in yeast; ssSPTs in mammals), is negatively regulated by the evolutionarily conserved family of proteins known as the ORMs. In yeast, SPT, the ORMs, and the PI4P phosphatase Sac1, copurify in the “SPOTs” complex. However, neither the ...
DOI:
10.1016/j.bbalip.2018.11.007
PubMed:
30529276
PubMed Central:
PMC6373489

3. Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology

Author:
Nissimov, Jozef I.; Talmy, David; Haramaty, Liti; Fredricks, Helen F.; Zelzion, Ehud; Knowles, Ben; Eren, A. Murat; Vandzura, Rebecca; Laber, Christien P.; Schieler, Brittany M.; Johns, Christopher T.; More, Kuldeep D.; Coolen, Marco J. L.; Follows, Michael J.; Bhattacharya, Debashish; Van Mooy, Benjamin A. S.; Bidle, Kay D.
Source:
Environmental microbiology 2019 v.21 no.6 pp. 2182-2197
ISSN:
1462-2912
Subject:
serine C-palmitoyltransferase,   etc   ; Emiliania huxleyi; biogeochemistry; biosynthesis; carbon; glycosphingolipids; host range; laboratory experimentation; virulence; virus replication; viruses;     Show all 11 Subjects
Abstract:
... Coccolithoviruses (EhVs) are large, double‐stranded DNA‐containing viruses that infect the single‐celled, marine coccolithophore Emiliania huxleyi. Given the cosmopolitan nature and global importance of E. huxleyi as a bloom‐forming, calcifying, photoautotroph, E. huxleyi–EhV interactions play a key role in oceanic carbon biogeochemistry. Virally‐encoded glycosphingolipids (vGSLs) are virulence fa ...
DOI:
10.1111/1462-2920.14633

4. Regulation of the amount of ceramide-1-phosphate synthesized in differentiated human podocytes

Author:
Mallela, Shamroop kumar; Mitrofanova, Alla; Merscher, Sandra; Fornoni, Alessia
Source:
Biochimica et biophysica acta 2019
ISSN:
1388-1981
Subject:
serine C-palmitoyltransferase,   etc   ; apoptosis; cell division; cell movement; cell proliferation; ceramides; filtration; gene expression regulation; homeostasis; humans; inflammation; kidneys; macrophages; membrane fluidity; plasma membrane; proteins; yeasts;     Show all 17 Subjects
Abstract:
... Sphingolipids have important functions as structural components of cells but they also function as signaling molecules regulating different cellular processes such as apoptosis, cell proliferation, cell migration, cell division and inflammation. Hence, a tight regulation of the sphingolipid homeostasis is essential to maintain proper cellular functions. Mammalian ORMDL proteins are orthologues of ...
DOI:
10.1016/j.bbalip.2019.158517
PubMed:
31487557
PubMed Central:
PMC6832884

5. Transmembrane topology of mammalian ORMDL proteins in the endoplasmic reticulum as revealed by the substituted cysteine accessibility method (SCAM™)

Author:
Davis, Deanna; Suemitsu, John; Wattenberg, Binks
Source:
Biochimica et biophysica acta 2019 v.1867 no.4 pp. 382-395
ISSN:
1570-9639
Subject:
serine C-palmitoyltransferase,   etc   ; amino acid sequences; biosynthesis; cysteine; cytosol; detergents; endoplasmic reticulum; hydrophobicity; mammals; membrane permeability; models; mutants; prediction; proteins; sphingolipids; topology;     Show all 16 Subjects
Abstract:
... Sphingolipids are diverse lipids with essential, and occasionally opposing, functions in the cell and therefore tight control over biosynthesis is vital. Mechanisms governing this regulation are not understood. Initial steps in sphingolipid biosynthesis take place on the cytosolic face of the endoplasmic reticulum (ER). Serine palmitoyltransferase (SPT) is an ER-resident enzyme catalyzing the firs ...
DOI:
10.1016/j.bbapap.2019.01.005
PubMed:
30639427
PubMed Central:
PMC6391742

6. Analysis of 1-Deoxysphingoid Bases and Their N-Acyl Metabolites and Exploration of Their Occurrence in Some Food Materials

Author:
Wan, Junliang; Li, Jian; Bandyopadhyay, Sibali; Kelly, Samuel L.; Xiang, Yang; Zhang, Jin; Merrill, Alfred H.; Duan, Jingjing
Source:
Journal of agricultural and food chemistry 2019 v.67 no.46 pp. 12953-12961
ISSN:
1520-5118
Subject:
serine C-palmitoyltransferase,   etc   ; acyl coenzyme A; alanine; antineoplastic agents; ceramides; diet; electrospray ionization mass spectrometry; foods; glycine (amino acid); human diseases; human health; mammals; metabolites; moieties; quantitative analysis; reversed-phase liquid chromatography; serine; sphinganine;     Show all 18 Subjects
Abstract:
... Most common sphingolipids are comprised of “typical” sphingoid bases (sphinganine, sphingosine, and structurally related compounds) and are produced via the condensation of l-serine with a fatty acyl-CoA by serine palmitoyltransferase. Some organisms, including mammals, also produce “atypical” sphingoid bases that lack a 1-hydroxyl group as a result of the utilization of l-alanine or glycine inste ...
DOI:
10.1021/acs.jafc.9b05708

7. De novo synthesis of sphingolipids is essential for decidualization in mice

Author:
Ding, Nai-Zheng; Qi, Qian-Rong; Gu, Xiao-Wei; Zuo, Ru-Juan; Liu, Jie; Yang, Zeng-Ming
Source:
Theriogenology 2018 v.106 pp. 227-236
ISSN:
0093-691X
Subject:
serine C-palmitoyltransferase,   etc   ; immune response; messenger RNA; mice; neurodevelopment; pregnancy; progesterone; signal transduction; sphingolipids; stress response; stromal cells; uterus;     Show all 12 Subjects
Abstract:
... Sphingolipids play multiple roles in membrane structure, signal transduction, stress responses, neural development and immune reaction. The rate of de novo synthesis pathway of sphingolipids is regulated by two key enzymes, serine palmitoyltransferase (SPT), and ketoreductase (Kds). Here, we find that the mRNA levels of three subunits of the SPT holoenzyme (Sptlc1, Sptlc2, and Ssspta) are signific ...
DOI:
10.1016/j.theriogenology.2017.09.036

8. Cardiac Lipid Metabolism Is Modulated by Casimiroa edulis and Crataegus pubescens Aqueous Extracts in High Fat and Fructose (HFF) Diet‐Fed Obese Rats

Author:
Pérez‐Ramírez, Iza F.; González‐Dávalos, María L.; Mora, Ofelia; Silva, Isaac; Gallegos‐Corona, Marco A.; Guzmán‐Maldonado, Salvador H.; Reynoso‐Camacho, Rosalía
Source:
European journal of lipid science and technology 2019 v.121 no.11 pp. e1900157
ISSN:
1438-7697
Subject:
serine C-palmitoyltransferase,   etc   ; Casimiroa edulis; Crataegus; acetyl-CoA carboxylase; acyl-CoA dehydrogenase; alkaloids; animal disease models; anti-inflammatory activity; antioxidant activity; beta oxidation; beverages; bioactive compounds; caffeic acid; cardioprotective effect; carnitine palmitoyltransferase; ceramides; chlorogenic acid; diacylglycerols; diet; fatty acid composition; fatty acids; fatty-acid synthase; fructose; genes; herbs; hypertrophy; lipogenesis; lipotoxicity; obesity; rats; steroid saponins;     Show all 31 Subjects
Abstract:
... This study aim to evaluate the effect of Casimiroa edulis and Crataegus pubescens aqueous extracts on cardiac lipotoxicity in obese rats. Both extracts decrease cardiac hypertrophy and steatosis in high fat and fructose (HFF)‐diet‐fed obese rats. C. pubescens decreases the cardiac content of 16 and 18 carbon‐length fatty acids, whereas C. edulis extract does not alter the fatty acid profile. Regar ...
DOI:
10.1002/ejlt.201900157

9. Sulforaphane Prevents Hepatic Insulin Resistance by Blocking Serine Palmitoyltransferase 3-Mediated Ceramide Biosynthesis

Author:
Teng, Wendi; Li, Yuan; Du, Min; Lei, Xingen; Xie, Siyu; Ren, Fazheng
Source:
Nutrients 2019 v.11 no.5
ISSN:
2072-6643
Subject:
serine C-palmitoyltransferase,   etc   ; Brassicaceae; biosynthesis; carcinoma; ceramides; clinical trials; gene expression regulation; genes; glucose; glucose tolerance; glycogen; homeostasis; human cell lines; humans; insulin; insulin receptor substrate proteins; insulin resistance; non-specific serine/threonine protein kinase; signal transduction; sulforaphane; transcription (genetics); triacylglycerols; vegetables;     Show all 23 Subjects
Abstract:
... Sulforaphane (SFA), a naturally active isothiocyanate compound from cruciferous vegetables used in clinical trials for cancer treatment, was found to possess potency to alleviate insulin resistance. But its underlying molecular mechanisms are still incompletely understood. In this study, we assessed whether SFA could improve insulin sensitivity and glucose homeostasis both in vitro and in vivo by ...
DOI:
10.3390/nu11051185
PubMed:
31137828
PubMed Central:
PMC6566605

10. Pharmacological characterization of synthetic serine palmitoyltransferase inhibitors by biochemical and cellular analyses

Author:
Adachi, Ryutaro; Asano, Yasutomi; Ogawa, Kazumasa; Oonishi, Motomi; Tanaka, Yukiya; Kawamoto, Tomohiro
Source:
Biochemical and biophysical research communications 2017
ISSN:
0006-291X
Subject:
serine C-palmitoyltransferase,   etc   ; ceramides; dissociation; endoplasmic reticulum; enzyme activity; humans; lung neoplasms; mechanism of action; serine;     Show all 9 Subjects
Abstract:
... Human serine palmitoyltransferase (SPT) is a PLP-dependent enzyme residing in the endoplasmic reticulum. It catalyzes the synthesis of 3-ketodihydrosphingosine (3-KDS) from the substrates palmitoyl-CoA and l-serine. It is a rate-limiting enzyme for sphingolipid synthesis in cells. In the present study, we characterized and pharmacologically profiled a series of tetrahydropyrazolopyridine derivativ ...
DOI:
10.1016/j.bbrc.2016.12.182

11. Antitumor activity of a novel and orally available inhibitor of serine palmitoyltransferase

Author:
Yaguchi, Masahiro; Shibata, Sachio; Satomi, Yoshinori; Hirayama, Megumi; Adachi, Ryutaro; Asano, Yasutomi; Kojima, Takuto; Hirata, Yasuhiro; Mizutani, Akio; Kiba, Atsushi; Sagiya, Yoji
Source:
Biochemical and biophysical research communications 2017
ISSN:
0006-291X
Subject:
serine C-palmitoyltransferase,   etc   ; antineoplastic activity; antineoplastic agents; biosynthesis; body weight changes; ceramides; cytotoxicity; mice; models; myeloid leukemia; neoplasm cells; sphingomyelins;     Show all 12 Subjects
Abstract:
... Metabolic reprogramming is an essential hallmark of neoplasia. Therefore, targeting cancer metabolism, including lipid synthesis, has attracted much interest in recent years. Serine palmitoyltransferase (SPT) plays a key role in the initial and rate-limiting step of de novo sphingolipid biosynthesis, and inhibiting SPT activity prevents the proliferation of certain cancer cells. Here, we identifie ...
DOI:
10.1016/j.bbrc.2017.01.075

12. Yeast lacking the amphiphysin family protein Rvs167 is sensitive to disruptions in sphingolipid levels

Author:
Toume, Moeko; Tani, Motohiro
Source:
The FEBS journal 2016 v.283 no.15 pp. 2911-2928
ISSN:
1742-464X
Subject:
serine C-palmitoyltransferase,   etc   ; Saccharomyces cerevisiae; biosynthesis; endocytosis; genes; guanosinetriphosphatase; microfilaments; phenotype; proteins; salt stress; sphingolipids; temperature; vacuoles; yeasts;     Show all 14 Subjects
Abstract:
... Rvs167 and Rvs161 in Saccharomyces cerevisiae are amphiphysin family proteins, which are involved in several important cellular events, such as invagination and scission of endocytic vesicles, and actin cytoskeleton organization. It has been reported that cellular dysfunctions caused by deletion of RVS167 or RVS161 are rescued by deletion of specific nonessential sphingolipid‐metabolizing enzyme g ...
DOI:
10.1111/febs.13783

13. Co-option of the sphingolipid metabolism for the production of nitroalkene defensive chemicals in termite soldiers

Author:
Jirošová, Anna; Jančařík, Andrej; Menezes, Riya C.; Bazalová, Olga; Dolejšová, Klára; Vogel, Heiko; Jedlička, Pavel; Buček, Aleš; Brabcová, Jana; Majer, Pavel; Hanus, Robert; Svatoš, Aleš
Source:
Insect biochemistry and molecular biology 2017 v.82 pp. 52-61
ISSN:
0965-1748
Subject:
serine C-palmitoyltransferase,   etc   ; Prorhinotermes; biochemical pathways; biosynthesis; carbon; chemical defenses; decarboxylation; genes; insects; nitro compounds; ontogeny; oxidation; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; sequence analysis; serine; sphingolipids;     Show all 17 Subjects
Abstract:
... The aliphatic nitroalkene (E)-1-nitropentadec-1-ene (NPD), reported in early seventies in soldiers of the termite genus Prorhinotermes, was the first documented nitro compound produced by insects. Yet, its biosynthetic origin has long remained unknown. Here, we investigated in detail the biosynthesis of NPD in P. simplex soldiers. First, we track the dynamics in major metabolic pathways during sol ...
DOI:
10.1016/j.ibmb.2017.01.008

14. The histone deacetylase-6 inhibitor tubacin directly inhibits de novo sphingolipid biosynthesis as an off-target effect

Author:
Siow, Deanna; Wattenberg, Binks
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 268-271
ISSN:
0006-291X
Subject:
serine C-palmitoyltransferase,   etc   ; acetylation; apoptosis; biosynthesis; cell communication; cell viability; histone deacetylase; histones; sphingolipids; sphingosine; tubulin;     Show all 11 Subjects
Abstract:
... Histone deacetylase 6 (HDAC6) controls acetylation of a number of cytosolic proteins, most prominently tubulin. Tubacin is a small molecule inhibitor of HDAC6 selected for its selective inhibition of HDAC6 relative to other histone deacetylases. For this reason it has become a useful pharmacological tool to discern the biological functions of HDAC6 in numerous cellular processes. The interest of t ...
DOI:
10.1016/j.bbrc.2014.05.016

15. Permanent draft genomes of four new coccolithoviruses: EhV-18, EhV-145, EhV-156 and EhV-164

Author:
Nissimov, Jozef I.; Napier, Johnathan A.; Kimmance, Susan A.; Allen, Michael J.
Source:
Marine genomics 2014 v.15 pp. 7-8
ISSN:
1874-7787
Subject:
serine C-palmitoyltransferase,   etc   ; Emiliania huxleyi; gene fusion; genes; microalgae; United Kingdom;     Show all 6 Subjects
Abstract:
... Coccolithoviruses infect the marine coccolithophorid microalga Emiliania huxleyi. Here, we describe the genomes of four new coccolithoviruses isolated from UK coastal locations. Of particular interest, EhV-18 and EhV-145 encode serine palmitoyltransferase function via two distinct genes, whereas all other coccolithoviruses have SPT as a gene fusion of LCB1/LCB2 domains. ...
DOI:
10.1016/j.margen.2014.02.004

16. Dietary supplement of conjugated linoleic acids or polyunsaturated fatty acids suppressed the mobilization of body fat reserves in dairy cows at early lactation through different pathways

Author:
Qin, Nanbing; Bayat, Ali-Reza; Trevisi, Erminio; Minuti, Andrea; Kairenius, Piia; Viitala, Sirja; Mutikainen, Mervi; Leskinen, Heidi; Elo, Kari; Kokkonen, Tuomo; Vilkki, Johanna
Source:
Journal of dairy science 2018 v.101 no.9 pp. 7954-7970
ISSN:
0022-0302
Subject:
serine C-palmitoyltransferase,   etc   ; 3-hydroxybutyric acid; Helianthus annuus; Toll-like receptor 4; adipophilin; adipose tissue; blood; body weight changes; carboxylic ester hydrolases; ceramides; conjugated linoleic acid; dairy cows; dry matter intake; early lactation; energy balance; fatty acid-binding proteins; fish oils; free fatty acids; gene expression; gene expression regulation; genes; glycerol-3-phosphate acyltransferase; inflammation; insulin; insulin resistance; lactose; lipid content; lipogenesis; lipolysis; mammary glands; milk; milk fat; milk fat yield; milk proteins; mitochondria; peroxisome proliferator-activated receptor gamma; polyunsaturated fatty acids; protein kinases; quantitative polymerase chain reaction; transcription (genetics);     Show all 40 Subjects
Abstract:
... To investigate the metabolic changes in the adipose tissue (AT) of dairy cows under milk fat depression (MFD), 30 cows were randomly allocated to a control diet, a conjugated linoleic acid (CLA)-supplemented diet, or a high-starch diet supplemented with a mixture of sunflower and fish oil (2:1; as HSO diet) from 1 to 112 d in milk. Performance of animals, milk yield, milk composition, energy balan ...
DOI:
10.3168/jds.2017-14298

17. Fiber Specific Changes in Sphingolipid Metabolism in Skeletal Muscles of Hyperthyroid Rats

Author:
Chabowski, A.; Żendzian-Piotrowska, M.; Mikłosz, A.; Łukaszuk, B.; Kurek, K.; Górski, J.
Source:
Lipids 2013 v.48 no.7 pp. 697-704
ISSN:
0024-4201
Subject:
serine C-palmitoyltransferase,   etc   ; ceramides; glycolysis; hydrolysis; hyperthyroidism; muscles; rats; skeletal muscle; sphingomyelins; sphingosine; thyroid hormones;     Show all 11 Subjects
Abstract:
... Thyroid hormones (T₃, T₄) are well known modulators of different cellular signals including the sphingomyelin pathway. However, studies regarding downstream effects of T₃ on sphingolipid metabolism in skeletal muscle are scarce. In the present work we sought to investigate the effects of hyperthyroidism on the activity of the key enzymes of ceramide metabolism as well as the content of fundamental ...
DOI:
10.1007/s11745-013-3769-3
PubMed:
23467817
PubMed Central:
PMC3690184

18. Mechanistic enzymology of serine palmitoyltransferase

Author:
Ikushiro, Hiroko; Hayashi, Hideyuki
Source:
Biochimica et biophysica acta 2011 v.1814 no.11 pp. 1474-1480
ISSN:
1878-1454
Subject:
serine C-palmitoyltransferase,   etc   ; biosynthesis; pyridoxal phosphate; sphingolipids; spectroscopy; enzymology;     Show all 6 Subjects
Abstract:
... Serine palmitoyltransferase, which is one of the α-oxamine synthase family enzymes, catalyzes the condensation reaction of L-serine and palmitoyl-CoA to form 3-ketodihydrosphingosine, the first and rate-determining step of the sphingolipid biosynthesis. As with other α-oxamine synthase family enzymes, the catalytic reaction is composed of multiple elementary steps, and the mechanism to control the ...
DOI:
10.1016/j.bbapap.2011.02.005

19. Reconstitution of the pyridoxal 5′-phosphate (PLP) dependent enzyme serine palmitoyltransferase (SPT) with pyridoxal reveals a crucial role for the phosphate during catalysis

Author:
Beattie, Ashley E.; Clarke, David J.; Wadsworth, John M.; Lowther, Jonathan; Sin, Ho-Lam; Campopiano, Dominic J.
Source:
Chemical communications 2013 v.49 no.63 pp. 7058-7060
ISSN:
1364-548X
Subject:
serine C-palmitoyltransferase,   etc   ; biosynthesis; catalytic activity; chemical communication; chemical reactions; moieties; phosphates; pyridoxal; pyridoxal phosphate; pyridoxine; serine; sphingolipids; substrate specificity;     Show all 13 Subjects
Abstract:
... The pyridoxal 5′-phosphate (PLP)-dependent enzyme serine palmitoyltransferase (SPT) is required for de novo sphingolipid biosynthesis. A previous study revealed a novel and unexpected interaction between the hydroxyl group of the l-serine substrate and the 5′-phosphate group of PLP. By using pyridoxal (PL), the dephosphorylated analogue of vitamin B₆, we show here that this interaction is importan ...
DOI:
10.1039/c3cc43001d

20. Intact sphingomyelin biosynthetic pathway is essential for intracellular transport of influenza virus glycoproteins

Author:
Tafesse, Fikadu G.; Sanyal, Sumana; Ashour, Joseph; Guimaraes, Carla P.; Hermansson, Martin; Somerharju, Pentti; Ploegh, Hidde L.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2013 v.110 no.16 pp. 6406-6411
ISSN:
0027-8424
Subject:
serine C-palmitoyltransferase,   etc   ; Orthomyxoviridae; antibodies; biochemical pathways; glycoproteins; hemagglutinins; influenza; physiological transport; sialidase; sphingomyelins; trypsin; viral proteins; virion; viruses;     Show all 14 Subjects
Abstract:
... Cells genetically deficient in sphingomyelin synthase-1 (SGMS1) or blocked in their synthesis pharmacologically through exposure to a serine palmitoyltransferase inhibitor (myriocin) show strongly reduced surface display of influenza virus glycoproteins hemagglutinin (HA) and neuraminidase (NA). The transport of HA to the cell surface was assessed by accessibility of HA on intact cells to exogenou ...
DOI:
10.1073/pnas.1219909110
PubMed:
23576732
PubMed Central:
PMC3631694