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1. Five structural genes required for ceramide synthesis in Caulobacter and for bacterial survival

Author:
Olea‐Ozuna, Roberto Jhonatan; Poggio, Sebastian; EdBergström,; Quiroz‐Rocha, Elva; García‐Soriano, Daniela A.; Sahonero‐Canavesi, Diana X.; Padilla‐Gómez, Jonathan; Martínez‐Aguilar, Lourdes; López‐Lara, Isabel M.; Thomas‐Oates, Jane; Geiger, Otto
Source:
Environmental microbiology 2021 v.23 no.1 pp. 143-159
ISSN:
1462-2912
Subject:
serine C-palmitoyltransferase,   etc   ; Caulobacter crescentus; bacteria; biosynthesis; cell differentiation; cell division; ceramides; genomics; mutants; structural genes;     Show all 10 Subjects
Abstract:
... Sphingolipids are essential and common membrane components in eukaryotic organisms, participating in many important cellular functions. Only a few bacteria are thought to harbour sphingolipids in their membranes, among them the well‐studied α‐proteobacterium Caulobacter crescentus, a model organism for asymmetric cell division and cellular differentiation. Here, we report that C. crescentus wild t ...
DOI:
10.1111/1462-2920.15280

2. The aryl hydrocarbon receptor activates ceramide biosynthesis in mice contributing to hepatic lipogenesis

Author:
Liu, Qing; Zhang, Limin; Allman, Erik L.; Hubbard, Troy D.; Murray, Iain A.; Hao, Fuhua; Tian, Yuan; Gui, Wei; Nichols, Robert G.; Smith, Philip B.; Anitha, Mallappa; Perdew, Gary H.; Patterson, Andrew D.
Source:
Toxicology 2021 v.458 pp. 152831
ISSN:
0300-483X
Subject:
serine C-palmitoyltransferase,   etc   ; aryl hydrocarbon receptors; biosynthesis; body weight; ceramides; chromatin immunoprecipitation; gel electrophoresis; genes; lipidomics; lipogenesis; luciferase; mass spectrometry; reaction kinetics; toxicology;     Show all 14 Subjects
Abstract:
... Aryl hydrocarbon receptor (AHR) activation via 2,3,7,8-tetrachlorodibenzofuran (TCDF) induces the accumulation of hepatic lipids. Here we report that AHR activation by TCDF (24 μg/kg body weight given orally for five days) induced significant elevation of hepatic lipids including ceramides in mice, was associated with increased expression of key ceramide biosynthetic genes, and increased activity ...
DOI:
10.1016/j.tox.2021.152831

3. Transformation of coccolithophorid Emiliania huxleyi harboring a marine virus (Coccolithoviruses) serine palmitoyltransferase (SPT) gene by electroporation

Author:
Cai, Weicong; Wang, Xueting; Su, Jinjing; Li, Jian; Zeng, Jun; Li, Guiling; Liu, Jingwen
Source:
Journal of oceanology and limnology 2021 v.39 no.2 pp. 693-704
ISSN:
2096-5508
Subject:
serine C-palmitoyltransferase,   etc   ; DNA; Emiliania huxleyi; Southern blotting; Western blotting; biochemical pathways; biogeochemistry; biosynthesis; chlorophyll; electroporation; eukaryotic cells; genes; genetic transformation; glufosinate; horizontal gene transfer; limnology; nuclear genome; oceanography; sphingolipids; thin layer chromatography; viruses;     Show all 21 Subjects
Abstract:
... Emiliania huxleyi is the most prominent modern coccolithophore, a group of marine unicellular eukaryotes that play a critical role in ocean biogeochemistry. Coccolithoviruses are large double stranded DNA viruses, which is responsible for the demise of large oceanic blooms formed by E. huxleyi. E. huxleyi virus (EhVs) acquired a series of enzyme-coding genes predicted to be involved in the sphingo ...
DOI:
10.1007/s00343-020-9325-0

4. Semi-rational approach to expand the Acyl-CoA Chain length tolerance of Sphingomonas paucimobilis serine palmitoyltransferase

Author:
Choe, Hyunjun; Cha, Minsun; Stewart, Jon D.
Source:
Enzyme and microbial technology 2020 v.137 pp. 109515
ISSN:
0141-0229
Subject:
serine C-palmitoyltransferase,   etc   ; Sphingomonas paucimobilis; active sites; acyl coenzyme A; aldimines; biosynthesis; carboxylic acids; decarboxylation; mutagenesis; mutants; sphingolipids; technology;     Show all 12 Subjects
Abstract:
... Serine palmitoyltransferase (SPTase), the first enzyme of the sphingolipid biosynthesis pathway, produces 3-ketodihydrosphingosine by a Claisen-like condensation/decarboxylation reaction of l-Ser and palmitoyl-CoA (n-C₁₆-CoA). Previous structural analysis of Sphingomonas paucimobilis SPTase (SpSPTase) revealed a dynamic active site loop (RPPATP; amino acids 378–383) in which R378 (underlined) form ...
DOI:
10.1016/j.enzmictec.2020.109515

5. Curcumin stimulates exosome/microvesicle release in an in vitro model of intracellular lipid accumulation by increasing ceramide synthesis

Author:
García-Seisdedos, David; Babiy, Bohdan; Lerma, Milagros; Casado, María E.; Martínez-Botas, Javier; Lasunción, Miguel A.; Pastor, Óscar; Busto, Rebeca
Source:
Biochimica et biophysica acta 2020 v.1865 no.5 pp. 158638
ISSN:
1388-1981
Subject:
serine C-palmitoyltransferase,   etc   ; Curcuma longa; animal models; ceramides; curcumin; enzyme inhibitors; exosomes; fumonisin B1; hydrophobicity; mice; neuroglia; polyphenols; rhizomes; secretion; sphingosine N-acyltransferase;     Show all 15 Subjects
Abstract:
... Curcumin, a hydrophobic polyphenol found in the rhizome of Curcuma longa, has been shown to reduce intracellular lipid accumulation in mouse models of lysosomal storage diseases such as Niemann-Pick type C. Exosomes are small extracellular vesicles secreted by cells in response to changes in intracellular ceramide composition. Curcumin can induce exosome/microvesicle release in cellular models of ...
DOI:
10.1016/j.bbalip.2020.158638

6. SPTLC1 inhibits cell growth via modulating Akt/FOXO1 pathway in renal cell carcinoma cells

Author:
Kong, Zhenzhen; Guo, Xinming; Zhao, Zhijian; Wu, Weizhou; Luo, Lianmin; Zhu, Zhiguo; Yin, Shanfeng; Cai, Chao; Wu, Wenqi; Wang, Ding; Liu, Yongda; Duan, Xiaolu
Source:
Biochemical and biophysical research communications 2019 v.520 no.1 pp. 1-7
ISSN:
0006-291X
Subject:
serine C-palmitoyltransferase,   etc   ; biosynthesis; cell growth; cell viability; neoplasm cells; renal cell carcinoma; research; sphingolipids;     Show all 8 Subjects
Abstract:
... Serine palmitoyltransferase long chain-1 (SPTLC1), which is the rate-limiting enzyme for sphingolipid biosynthesis, has been indicated to be essential for carcinoma cell survival and proliferation in recent, but its role in the regulation of renal cell carcinoma (RCC) remains unknown. In the present study, we found that SPTLC1 expression was significantly decreased in RCC tissues compared to non-t ...
DOI:
10.1016/j.bbrc.2019.09.073

7. The ORMs interact with transmembrane domain 1 of Lcb1 and regulate serine palmitoyltransferase oligomerization, activity and localization

Author:
Han, Gongshe; Gupta, Sita D.; Gable, Kenneth; Bacikova, Dagmar; Sengupta, Nivedita; Somashekarappa, Niranjanakumari; Proia, Richard L.; Harmon, Jeffrey M.; Dunn, Teresa M.
Source:
Biochimica et biophysica acta 2019 v.1864 no.3 pp. 245-259
ISSN:
1388-1981
Subject:
serine C-palmitoyltransferase,   etc   ; image analysis; mammals; mutants; oligomerization; phosphorylation; precipitin tests; proteins; topology; yeasts;     Show all 10 Subjects
Abstract:
... Serine palmitoyltransferase (SPT), an endoplasmic reticulum-localized membrane enzymecomposed of acatalytic LCB1/LCB2 heterodimer and a small activating subunit (Tsc3 in yeast; ssSPTs in mammals), is negatively regulated by the evolutionarily conserved family of proteins known as the ORMs. In yeast, SPT, the ORMs, and the PI4P phosphatase Sac1, copurify in the “SPOTs” complex. However, neither the ...
DOI:
10.1016/j.bbalip.2018.11.007
PubMed:
30529276
PubMed Central:
PMC6373489

8. Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology

Author:
Nissimov, Jozef I.; Talmy, David; Haramaty, Liti; Fredricks, Helen F.; Zelzion, Ehud; Knowles, Ben; Eren, A. Murat; Vandzura, Rebecca; Laber, Christien P.; Schieler, Brittany M.; Johns, Christopher T.; More, Kuldeep D.; Coolen, Marco J. L.; Follows, Michael J.; Bhattacharya, Debashish; Van Mooy, Benjamin A. S.; Bidle, Kay D.
Source:
Environmental microbiology 2019 v.21 no.6 pp. 2182-2197
ISSN:
1462-2912
Subject:
serine C-palmitoyltransferase,   etc   ; Emiliania huxleyi; biogeochemistry; biosynthesis; carbon; glycosphingolipids; host range; laboratory experimentation; virulence; virus replication; viruses;     Show all 11 Subjects
Abstract:
... Coccolithoviruses (EhVs) are large, double‐stranded DNA‐containing viruses that infect the single‐celled, marine coccolithophore Emiliania huxleyi. Given the cosmopolitan nature and global importance of E. huxleyi as a bloom‐forming, calcifying, photoautotroph, E. huxleyi–EhV interactions play a key role in oceanic carbon biogeochemistry. Virally‐encoded glycosphingolipids (vGSLs) are virulence fa ...
DOI:
10.1111/1462-2920.14633

9. Regulation of the amount of ceramide-1-phosphate synthesized in differentiated human podocytes

Author:
Mallela, Shamroop kumar; Mitrofanova, Alla; Merscher, Sandra; Fornoni, Alessia
Source:
Biochimica et biophysica acta 2019
ISSN:
1388-1981
Subject:
serine C-palmitoyltransferase,   etc   ; apoptosis; cell division; cell movement; cell proliferation; ceramides; filtration; gene expression regulation; homeostasis; humans; inflammation; kidneys; macrophages; membrane fluidity; plasma membrane; proteins; yeasts;     Show all 17 Subjects
Abstract:
... Sphingolipids have important functions as structural components of cells but they also function as signaling molecules regulating different cellular processes such as apoptosis, cell proliferation, cell migration, cell division and inflammation. Hence, a tight regulation of the sphingolipid homeostasis is essential to maintain proper cellular functions. Mammalian ORMDL proteins are orthologues of ...
DOI:
10.1016/j.bbalip.2019.158517
PubMed:
31487557
PubMed Central:
PMC6832884

10. Transmembrane topology of mammalian ORMDL proteins in the endoplasmic reticulum as revealed by the substituted cysteine accessibility method (SCAM™)

Author:
Davis, Deanna; Suemitsu, John; Wattenberg, Binks
Source:
Biochimica et biophysica acta 2019 v.1867 no.4 pp. 382-395
ISSN:
1570-9639
Subject:
serine C-palmitoyltransferase,   etc   ; amino acid sequences; biosynthesis; cysteine; cytosol; detergents; endoplasmic reticulum; hydrophobicity; mammals; membrane permeability; models; mutants; prediction; proteins; sphingolipids; topology;     Show all 16 Subjects
Abstract:
... Sphingolipids are diverse lipids with essential, and occasionally opposing, functions in the cell and therefore tight control over biosynthesis is vital. Mechanisms governing this regulation are not understood. Initial steps in sphingolipid biosynthesis take place on the cytosolic face of the endoplasmic reticulum (ER). Serine palmitoyltransferase (SPT) is an ER-resident enzyme catalyzing the firs ...
DOI:
10.1016/j.bbapap.2019.01.005
PubMed:
30639427
PubMed Central:
PMC6391742

11. De novo synthesis of sphingolipids is essential for decidualization in mice

Author:
Ding, Nai-Zheng; Qi, Qian-Rong; Gu, Xiao-Wei; Zuo, Ru-Juan; Liu, Jie; Yang, Zeng-Ming
Source:
Theriogenology 2018 v.106 pp. 227-236
ISSN:
0093-691X
Subject:
serine C-palmitoyltransferase,   etc   ; immune response; messenger RNA; mice; neurodevelopment; pregnancy; progesterone; signal transduction; sphingolipids; stress response; stromal cells; uterus;     Show all 12 Subjects
Abstract:
... Sphingolipids play multiple roles in membrane structure, signal transduction, stress responses, neural development and immune reaction. The rate of de novo synthesis pathway of sphingolipids is regulated by two key enzymes, serine palmitoyltransferase (SPT), and ketoreductase (Kds). Here, we find that the mRNA levels of three subunits of the SPT holoenzyme (Sptlc1, Sptlc2, and Ssspta) are signific ...
DOI:
10.1016/j.theriogenology.2017.09.036

12. Pharmacological characterization of synthetic serine palmitoyltransferase inhibitors by biochemical and cellular analyses

Author:
Adachi, Ryutaro; Asano, Yasutomi; Ogawa, Kazumasa; Oonishi, Motomi; Tanaka, Yukiya; Kawamoto, Tomohiro
Source:
Biochemical and biophysical research communications 2017
ISSN:
0006-291X
Subject:
serine C-palmitoyltransferase,   etc   ; ceramides; dissociation; endoplasmic reticulum; enzyme activity; humans; lung neoplasms; mechanism of action; serine;     Show all 9 Subjects
Abstract:
... Human serine palmitoyltransferase (SPT) is a PLP-dependent enzyme residing in the endoplasmic reticulum. It catalyzes the synthesis of 3-ketodihydrosphingosine (3-KDS) from the substrates palmitoyl-CoA and l-serine. It is a rate-limiting enzyme for sphingolipid synthesis in cells. In the present study, we characterized and pharmacologically profiled a series of tetrahydropyrazolopyridine derivativ ...
DOI:
10.1016/j.bbrc.2016.12.182

13. Analysis of 1-Deoxysphingoid Bases and Their N-Acyl Metabolites and Exploration of Their Occurrence in Some Food Materials

Author:
Wan, Junliang; Li, Jian; Bandyopadhyay, Sibali; Kelly, Samuel L.; Xiang, Yang; Zhang, Jin; Merrill, Alfred H.; Duan, Jingjing
Source:
Journal of agricultural and food chemistry 2019 v.67 no.46 pp. 12953-12961
ISSN:
1520-5118
Subject:
serine C-palmitoyltransferase,   etc   ; acyl coenzyme A; alanine; antineoplastic agents; ceramides; diet; electrospray ionization mass spectrometry; foods; glycine (amino acid); human diseases; human health; mammals; metabolites; moieties; quantitative analysis; reversed-phase liquid chromatography; serine; sphinganine;     Show all 18 Subjects
Abstract:
... Most common sphingolipids are comprised of “typical” sphingoid bases (sphinganine, sphingosine, and structurally related compounds) and are produced via the condensation of l-serine with a fatty acyl-CoA by serine palmitoyltransferase. Some organisms, including mammals, also produce “atypical” sphingoid bases that lack a 1-hydroxyl group as a result of the utilization of l-alanine or glycine inste ...
DOI:
10.1021/acs.jafc.9b05708

14. Knockout of neutrophil elastase protects against western diet induced nonalcoholic steatohepatitis in mice by regulating hepatic ceramides metabolism

Author:
Chen, Jin; Liang, Bingtian; Bian, Dongxue; Luo, Yan; Yang, Jing; Li, Zhihui; Zhuang, Zhengjie; Zang, Shufei; Shi, Junping
Source:
Biochemical and biophysical research communications 2019 v.518 no.4 pp. 691-697
ISSN:
0006-291X
Subject:
serine C-palmitoyltransferase,   etc   ; ; Western blotting; Western diets; blood serum; body weight; ceramides; elastase; fatty liver; fluorescent antibody technique; genes; hepatocytes; immunohistochemistry; liver; low fat diet; models; neutrophils; protein synthesis; quantitative polymerase chain reaction; research; triacylglycerols;     Show all 21 Subjects
Abstract:
... Previous studies reported increased expression and activity of neutrophil elastase (NE) in NASH. However, the role of NE in nonalcoholic steatohepatitis (NASH) remained unclear. Wild type (WT) and NE knockout (NE KO) mice were fed with western diet (WD) for 24 weeks to establish NASH model. The severity of liver injury in NASH mice was assessed by biochemical analysis, liver triglyceride (TG) quan ...
DOI:
10.1016/j.bbrc.2019.08.111

15. Antitumor activity of a novel and orally available inhibitor of serine palmitoyltransferase

Author:
Yaguchi, Masahiro; Shibata, Sachio; Satomi, Yoshinori; Hirayama, Megumi; Adachi, Ryutaro; Asano, Yasutomi; Kojima, Takuto; Hirata, Yasuhiro; Mizutani, Akio; Kiba, Atsushi; Sagiya, Yoji
Source:
Biochemical and biophysical research communications 2017
ISSN:
0006-291X
Subject:
serine C-palmitoyltransferase,   etc   ; antineoplastic activity; antineoplastic agents; biosynthesis; body weight changes; ceramides; cytotoxicity; mice; models; myeloid leukemia; neoplasm cells; sphingomyelins;     Show all 12 Subjects
Abstract:
... Metabolic reprogramming is an essential hallmark of neoplasia. Therefore, targeting cancer metabolism, including lipid synthesis, has attracted much interest in recent years. Serine palmitoyltransferase (SPT) plays a key role in the initial and rate-limiting step of de novo sphingolipid biosynthesis, and inhibiting SPT activity prevents the proliferation of certain cancer cells. Here, we identifie ...
DOI:
10.1016/j.bbrc.2017.01.075

16. Cardiac Lipid Metabolism Is Modulated by Casimiroa edulis and Crataegus pubescens Aqueous Extracts in High Fat and Fructose (HFF) Diet‐Fed Obese Rats

Author:
Pérez‐Ramírez, Iza F.; González‐Dávalos, María L.; Mora, Ofelia; Silva, Isaac; Gallegos‐Corona, Marco A.; Guzmán‐Maldonado, Salvador H.; Reynoso‐Camacho, Rosalía
Source:
European journal of lipid science and technology 2019 v.121 no.11 pp. e1900157
ISSN:
1438-7697
Subject:
serine C-palmitoyltransferase,   etc   ; Casimiroa edulis; Crataegus; acetyl-CoA carboxylase; acyl-CoA dehydrogenase; alkaloids; animal disease models; anti-inflammatory activity; antioxidant activity; beta oxidation; beverages; bioactive compounds; caffeic acid; cardioprotective effect; carnitine palmitoyltransferase; ceramides; chlorogenic acid; diacylglycerols; diet; fatty acid composition; fatty acids; fatty-acid synthase; fructose; genes; herbs; hypertrophy; lipogenesis; lipotoxicity; obesity; rats; steroid saponins;     Show all 31 Subjects
Abstract:
... This study aim to evaluate the effect of Casimiroa edulis and Crataegus pubescens aqueous extracts on cardiac lipotoxicity in obese rats. Both extracts decrease cardiac hypertrophy and steatosis in high fat and fructose (HFF)‐diet‐fed obese rats. C. pubescens decreases the cardiac content of 16 and 18 carbon‐length fatty acids, whereas C. edulis extract does not alter the fatty acid profile. Regar ...
DOI:
10.1002/ejlt.201900157

17. Sulforaphane Prevents Hepatic Insulin Resistance by Blocking Serine Palmitoyltransferase 3-Mediated Ceramide Biosynthesis

Author:
Teng, Wendi; Li, Yuan; Du, Min; Lei, Xingen; Xie, Siyu; Ren, Fazheng
Source:
Nutrients 2019 v.11 no.5
ISSN:
2072-6643
Subject:
serine C-palmitoyltransferase,   etc   ; Brassicaceae; biosynthesis; carcinoma; ceramides; clinical trials; gene expression regulation; genes; glucose; glucose tolerance; glycogen; homeostasis; human cell lines; humans; insulin; insulin receptor substrate proteins; insulin resistance; non-specific serine/threonine protein kinase; signal transduction; sulforaphane; transcription (genetics); triacylglycerols; vegetables;     Show all 23 Subjects
Abstract:
... Sulforaphane (SFA), a naturally active isothiocyanate compound from cruciferous vegetables used in clinical trials for cancer treatment, was found to possess potency to alleviate insulin resistance. But its underlying molecular mechanisms are still incompletely understood. In this study, we assessed whether SFA could improve insulin sensitivity and glucose homeostasis both in vitro and in vivo by ...
DOI:
10.3390/nu11051185
PubMed:
31137828
PubMed Central:
PMC6566605

18. Yeast lacking the amphiphysin family protein Rvs167 is sensitive to disruptions in sphingolipid levels

Author:
Toume, Moeko; Tani, Motohiro
Source:
The FEBS journal 2016 v.283 no.15 pp. 2911-2928
ISSN:
1742-464X
Subject:
serine C-palmitoyltransferase,   etc   ; Saccharomyces cerevisiae; biosynthesis; endocytosis; genes; guanosinetriphosphatase; microfilaments; phenotype; proteins; salt stress; sphingolipids; temperature; vacuoles; yeasts;     Show all 14 Subjects
Abstract:
... Rvs167 and Rvs161 in Saccharomyces cerevisiae are amphiphysin family proteins, which are involved in several important cellular events, such as invagination and scission of endocytic vesicles, and actin cytoskeleton organization. It has been reported that cellular dysfunctions caused by deletion of RVS167 or RVS161 are rescued by deletion of specific nonessential sphingolipid‐metabolizing enzyme g ...
DOI:
10.1111/febs.13783

19. The histone deacetylase-6 inhibitor tubacin directly inhibits de novo sphingolipid biosynthesis as an off-target effect

Author:
Siow, Deanna; Wattenberg, Binks
Source:
Biochemical and biophysical research communications 2014 v.449 pp. 268-271
ISSN:
0006-291X
Subject:
serine C-palmitoyltransferase,   etc   ; acetylation; apoptosis; biosynthesis; cell communication; cell viability; histone deacetylase; histones; sphingolipids; sphingosine; tubulin;     Show all 11 Subjects
Abstract:
... Histone deacetylase 6 (HDAC6) controls acetylation of a number of cytosolic proteins, most prominently tubulin. Tubacin is a small molecule inhibitor of HDAC6 selected for its selective inhibition of HDAC6 relative to other histone deacetylases. For this reason it has become a useful pharmacological tool to discern the biological functions of HDAC6 in numerous cellular processes. The interest of t ...
DOI:
10.1016/j.bbrc.2014.05.016

20. Permanent draft genomes of four new coccolithoviruses: EhV-18, EhV-145, EhV-156 and EhV-164

Author:
Nissimov, Jozef I.; Napier, Johnathan A.; Kimmance, Susan A.; Allen, Michael J.
Source:
Marine genomics 2014 v.15 pp. 7-8
ISSN:
1874-7787
Subject:
serine C-palmitoyltransferase,   etc   ; Emiliania huxleyi; gene fusion; genes; microalgae; United Kingdom;     Show all 6 Subjects
Abstract:
... Coccolithoviruses infect the marine coccolithophorid microalga Emiliania huxleyi. Here, we describe the genomes of four new coccolithoviruses isolated from UK coastal locations. Of particular interest, EhV-18 and EhV-145 encode serine palmitoyltransferase function via two distinct genes, whereas all other coccolithoviruses have SPT as a gene fusion of LCB1/LCB2 domains. ...
DOI:
10.1016/j.margen.2014.02.004