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1. Orchids - discoverers and firsts

Author:
Joseph Arditti
Source:
South African journal of botany 2022 v.146 pp. 815-848
ISSN:
0254-6299
Subject:
serology,   etc   ; Orchidaceae; biotechnology; people; plantlets; politics; viruses;     Show all 7 Subjects
Abstract:
... When Dr. Paromik Bhattacharyya first asked me to write for this special issue of the South African Journal of Botany, I refused for several good reasons: Retired for 20 years, not having done research for 21 years, 89 1/2 years old, no longer reading as extensively as before my retirement, recovering from surgeries, trying to manage health problems, which even if not severe are vexing, and being l ...
DOI:
10.1016/j.sajb.2022.01.029

2. Biosensors, modern technology for the detection of cancer-associated bacteria

Author:
Ahmad Mobed; Shirin Malehmir; Ali Ahmad alipour; Yasaman Azizimoghaddam; Hediyeh Saghi Sarabi; Farhood Ghazi
Source:
Biotechnology letters 2022 v.44 no.5-6 pp. 683-701
ISSN:
0141-5492
Subject:
serology,   etc   ; biosensors; biotechnology; humans; quantitative polymerase chain reaction;     Show all 5 Subjects
Abstract:
... Cancer is undoubtedly one of the major human challenges worldwide. A number of pathogenic bacteria are deemed to be potentially associated with the disease. Accordingly, accurate and specific identification of cancer-associated bacteria can play an important role in cancer control and prevention. A variety of conventional methods such as culture, serology, and molecular-based methods as well as PC ...
DOI:
10.1007/s10529-022-03257-8

3. An automated high-throughput enterovirus D68 microneutralization assay platform

Author:
Eric E. Rhoden; Bernardo A. Mainou; Jennifer L. Konopka-Anstadt; M. Steven Oberste
Source:
Journal of virological methods 2022 v.308 pp. 114590
ISSN:
0166-0934
Subject:
serology,   etc   ; Enterovirus; automation; humans; luminescent assay; neutralization tests; seroprevalence; viruses;     Show all 8 Subjects
Abstract:
... Virus neutralization assays, widely used to detect and quantify antibodies induced by virus infection, are considered the gold standard for enterovirus serology testing. Conventional microneutralization assays have been used to assess enterovirus D68 (EV-D68) seroprevalence. While manual or automated 96-well assays are valuable, higher-density assays that increase throughput provide the opportunit ...
DOI:
10.1016/j.jviromet.2022.114590

4. New insights on Leptospira sp. infection in ewes maintained in field semiarid conditions

Author:
Julio Cesar de Araujo Santos; Igor Felipe Ferreira de Vasconcelos; Denise Batista Nogueira; Joao Pessoa Araujo Junior; Camila Dantas Malossi; Leila Sabrina Ullmann; Carolina de Sousa Américo Batista Santos; Clebert José Alves; Maria Luana Cristiny Rodrigues Silva; Sérgio Santos de Azevedo
Source:
Acta tropica 2022 v.234 pp. 106610
ISSN:
0001-706X
Subject:
serology,   etc   ; DNA; agglutination tests; blood; herds; leptospirosis; polymerase chain reaction; urine;     Show all 8 Subjects
Abstract:
... Leptospirosis is poorly studied in small ruminants raised in field semiarid conditions. In this study we compared serological, bacteriological and molecular diagnostic methods in ewes maintained in field Brazilian semiarid conditions. Blood, vaginal fluid and urine samples were collected from 60 Morada Nova ewes raised in a semi-intensive system in the Brazilian semiarid. Diagnostic tests performe ...
DOI:
10.1016/j.actatropica.2022.106610

5. Micronutrient deficiencies are frequent in adult patients with and without celiac disease on a gluten-free diet, regardless of duration and adherence to the diet

Author:
Aseera Jivraj; Joy M. Hutchinson; Emily Ching; Avantika Marwaha; Elena F. Verdu; David Armstrong; M. Ines Pinto-Sanchez
Source:
Nutrition 2022 v.103-104 no. pp. 111809
ISSN:
0899-9007
Subject:
serology,   etc   ; adults; blood; celiac disease; compliance; ferritin; gluten-free diet; zinc;     Show all 8 Subjects
Abstract:
... The only treatment for celiac disease (CD) is a strict gluten-free diet (GFD). Nutritional deficiencies are common in CD; however, how this is influenced by the presence of symptoms, duration of CD, and compliance of GFD is less clear. The objective of this study was to compare nutritional deficiencies in patients with CD to those of nonceliac populations on a GFD. The secondary outcomes were to c ...
DOI:
10.1016/j.nut.2022.111809

6. Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material

Author:
James Freeman; Justin Conklin
Source:
Journal of virological methods 2022 v.300 pp. 114430
ISSN:
0166-0934
Subject:
serology,   etc   ; Severe acute respiratory syndrome coronavirus 2; antibodies; antigens; humans; vaccines;     Show all 6 Subjects
Abstract:
... WHO 20/136 is standard reference material for SARS−COV-2 serology assays. Standardization of serology assays that target the same antigen and class of immunoglobulin will enable comparison of results between studies that use various lab-developed and commercial assays around the world. Standardization of assays will help better define immune correlates of protection and possibly immune correlates ...
DOI:
10.1016/j.jviromet.2021.114430

7. Diagnostic Accuracy of LDBIO-Toxo II IgG and IgM Western Blot in Suspected Seroconversion in Pregnancy: A Multicentre Study

Author:
Valeria Meroni; Francesca Genco; Luigia Scudeller; Marie-Pierre Brenier-Pinchart; Hélène Fricker-Hidalgo; Coralie L’Ollivier; Luc Paris; Hervé Pelloux
Source:
Pathogens 2022 v.11 no.6 pp. -
ISSN:
2076-0817
Subject:
serology,   etc   ; Toxoplasma gondii; Western blotting; automation; diagnostic techniques; pregnancy; seroconversion; therapeutics; France;     Show all 9 Subjects
Abstract:
... The high sensitivity of the automated tests used for Toxoplasma gondii serology can yield false-positive IgM results due to aspecific reactions. On the other hand, specific therapy can delay IgG production and, therefore, the diagnosis of seroconversion. There is a need for confirmation tests to early detect seroconversions during pregnancy. We conducted a multicentre study to evaluate the diagnos ...
DOI:
10.3390/pathogens11060665

8. Primate hemorrhagic fever-causing arteriviruses are poised for spillover to humans

Author:
Cody J. Warren; Shuiqing Yu; Douglas K. Peters; Arturo Barbachano-Guerrero; Qing Yang; Bridget L. Burris; Gabriella Worwa; I-Chueh Huang; Gregory K. Wilkerson; Tony L. Goldberg; Jens H. Kuhn; Sara L. Sawyer
Source:
Cell 2022 pp. -
ISSN:
0092-8674
Subject:
serology,   etc   ; Alphaarterivirus; Deltaarterivirus hemfev; humans; monitoring; monocytes; new species; virus replication; viruses;     Show all 9 Subjects
Abstract:
... Simian arteriviruses are endemic in some African primates and can cause fatal hemorrhagic fevers when they cross into primate hosts of new species. We find that CD163 acts as an intracellular receptor for simian hemorrhagic fever virus (SHFV; a simian arterivirus), a rare mode of virus entry that is shared with other hemorrhagic fever-causing viruses (e.g., Ebola and Lassa viruses). Further, SHFV ...
DOI:
10.1016/j.cell.2022.09.022

9. Misleading Positive Serology for Cat Scratch Disease following Administration of Intravenous Immunoglobulin

Author:
Michal Yakubovsky; Yoav Golan; Alex Guri; Itzhak Levy; Daniel Glikman; Moshe Ephros; Michael Giladi
Source:
Pathogens 2022 v.11 no.2 pp. -
ISSN:
2076-0817
Subject:
serology,   etc   ; Bartonella henselae; blood serum; cat scratch disease; intravenous injection; patients; seroprevalence;     Show all 7 Subjects
Abstract:
... Cat scratch disease (CSD), caused by Bartonella henselae, usually presents as regional lymphadenopathy/lymphadenitis, known as typical CSD or as atypical CSD, which includes, among others, neurological manifestations. Serology for anti-B. henselae IgG antibodies is the most commonly used diagnostic tests for CSD. Intravenous immunoglobulin (IVIG) is given for an increasing number of medical condit ...
DOI:
10.3390/pathogens11020177

10. The value of West Nile virus RNA detection by real-time RT-PCR in urine samples from patients with neuroinvasive forms

Author:
Mariem Gdoura; Wasfi Fares; Souha Bougatef; Amine Inoubli; Henda Touzi; Nahed Hogga; Imen Ben Dhifallah; Naila Hannachi; Aida Argoubi; Saoussen Kacem; Hela Karray; Nissaf Ben Alaya; Henda Triki
Source:
Archives of microbiology 2022 v.204 no.5 pp. 238
ISSN:
0302-8933
Subject:
serology,   etc   ; RNA; West Nile virus; blood; cerebrospinal fluid; diagnostic techniques; microbiology; urine; Tunisia;     Show all 9 Subjects
Abstract:
... INTRODUCTION: Routine laboratory screening is based on the detection of WNV specific IgM and IgG in blood and cerebrospinal fluid. Confirmation is then classically applied by real time RT-PCR (rRT-PCR) in Cerebrospinal fluid (CSF), which often gives negative results due to too short virorachia and late sampling. rRT-PCR was applied-for the first time for routine diagnosis purpose-on urine samples. ...
DOI:
10.1007/s00203-022-02829-6

11. Bartonella spp. seroepidemiology and associations with clinicopathologic findings in dogs in the United States

Author:
Erin Lashnits; Brendon Thatcher; Ariel Carruth; Anton Mestek; Jesse Buch; Melissa Beall; Pradeep Neupane; Ramaswamy Chandrashekar; Edward B. Breitschwerdt
Source:
Journal of veterinary internal medicine 2022 v.36 no.1 pp. 116-125
ISSN:
0891-6640
Subject:
serology,   etc   ; blood chemistry; blood serum; confidence interval; odds ratio; serological surveys; veterinary medicine;     Show all 7 Subjects
Abstract:
... BACKGROUND: Improved understanding of Bartonella spp. serology in dogs may aid clinical decision making. OBJECTIVE: Describe demographic and geographic patterns of Bartonella spp. seroreactivity in dogs, and describe hematologic and serum biochemical abnormalities in Bartonella spp. seroreactive and nonseroreactive dogs. ANIMALS: Serum samples from 5957 dogs in the United States, previously submit ...
DOI:
10.1111/jvim.16311

12. The prevalence of Salmonella spp. in working farm dogs and their home-kill raw meat diets in Manawatū, New Zealand

Author:
K Bojanić; E Acke; PJ Biggs; AC Midwinter
Source:
New Zealand veterinary journal 2022 v.70 no.4 pp. 233-237
ISSN:
1176-0710
Subject:
serology,   etc   ; Salmonella; agar; dogs; farms; feces; hygiene; meat; peptones; raw meat; risk; New Zealand;     Show all 12 Subjects
Abstract:
... To investigate the prevalence of Salmonella spp. in a convenience sample of working farm dogs and their home-kill raw meat diets in Manawatū, New Zealand. Fifty farms in the Manawatū, with at least three working/herding dogs per farm that were fed raw home-killed meat at least fortnightly, were visited. One sample of dog faeces and one sample of food were collected per farm using convenience sampl ...
DOI:
10.1080/00480169.2022.2064929

13. Antibodies against Two Testudinid Herpesviruses in Pet Tortoises in Europe

Author:
Christoph Leineweber; Rachel E. Marschang
Source:
Animals 2022 v.12 no.17 pp. -
ISSN:
2076-2615
Subject:
serology,   etc   ; Herpesvirales; antibody detection; blood serum; hosts; neutralization tests; retrospective studies; summer; tortoises; viruses; Italy;     Show all 11 Subjects
Abstract:
... Herpesviruses are important pathogens of tortoises, and several serologically and genetically distinct virus types have been described in these animals. Virus neutralization testing is commonly used in Europe to determine previous infection with the two types most often found in pet European tortoises, testudinid herpesvirus (TeHV) 1 and 3. In this retrospective study, the results of serological t ...
DOI:
10.3390/ani12172298

14. Whole blood based point-of-care assay for the detection of anti-pertussis toxin IgG antibodies

Author:
Aapo Knuutila; Carita Rautanen; Alex-Mikael Barkoff; Jussi Mertsola; Qiushui He
Source:
Journal of immunological methods 2022 v.510 pp. 113361
ISSN:
0022-1759
Subject:
serology,   etc   ; blood serum; detection limit; fluorescence; monitoring; point-of-care systems; secondary immunization; serodiagnosis; toxins;     Show all 9 Subjects
Abstract:
... Current serological diagnosis of pertussis is usually done by ELISA to determine serum specific anti-pertussis toxin (PT) IgG antibodies. However, the ELISAs are often central-laboratory based, require trained staff, and have long turnaround times. A rapid point-of-care (POC) assay for pertussis serology would aid in both diagnosis and surveillance of the disease. In this study, a quantitative lat ...
DOI:
10.1016/j.jim.2022.113361

15. An update on spotted fever group serology in Kerman Province, Iran

Author:
Ali Qorbani; Mohammad Khalili; Saeidreza Nourollahifard; Ehsan Mostafavi; Mehrdad Farrokhnia; Saber Esmaeili
Source:
Comparative immunology, microbiology, and infectious diseases 2022 v.88 pp. 101862
ISSN:
0147-9571
Subject:
serology,   etc   ; Rickettsia conorii; blood; boutonneuse fever; etiological agents; fever; geographical distribution; health services; microbiology; seroprevalence; Iran;     Show all 11 Subjects
Abstract:
... Rickettsia conorii is the causative agent of Mediterranean spotted fever (MSF) and reports of its geographical distribution are spreading every day. Recently, some cases of the MSF have been reported from Iran. However, little information is available about the MSF epidemiology in Iran. The aim of the current study was to investigate anti-R. conorii IgG seroprevalence among farmers living in the r ...
DOI:
10.1016/j.cimid.2022.101862

16. Screening of wild deer populations for exposure to SARS‐CoV‐2 in the United Kingdom, 2020–2021

Author:
Maya Holding; Ashley David Otter; Stuart Dowall; Katsuhisa Takumi; Bethany Hicks; Tom Coleman; Georgia Hemingway; Matthew Royds; Stephen Findlay‐Wilson; Mollie Curran‐French; Richard Vipond; Hein Sprong; Roger Hewson
Source:
Transboundary and emerging diseases 2022 v.69 no.5 pp. e3244
ISSN:
1865-1674
Subject:
serology,   etc   ; Odocoileus virginianus; Severe acute respiratory syndrome coronavirus 2; deer; human population; hybrids; neutralization; United Kingdom;     Show all 8 Subjects
Abstract:
... Following findings in Northern America of SARS‐CoV‐2 infections in white‐tailed deer, there is concern of similar infections in European deer and their potential as reservoirs of SARS‐CoV‐2 including opportunities for the emergence of new variants. UK deer sera were collected in 2020–2021 from 6 species and a hybrid with 1748 tested using anti‐spike and anti‐nucleocapsid serology assays. No sample ...
DOI:
10.1111/tbed.14534

17. An eight-plex immunoassay for Group A streptococcus serology and vaccine development

Author:
Alana L. Whitcombe; Franklin Han; Sonia M. McAlister; Lea-Ann S. Kirkham; Paul G. Young; Stephen R. Ritchie; Polly Atatoa Carr; Thomas Proft; Nicole J. Moreland
Source:
Journal of immunological methods 2022 v.500 pp. 113194
ISSN:
0022-1759
Subject:
serology,   etc   ; Streptococcus; animal pathogens; antibodies; antigens; bacteremia; blood serum; carbohydrates; deoxyribonucleases; fever; immunoassays; vaccine development; vaccines;     Show all 13 Subjects
Abstract:
... Group A Streptococcus (GAS) is a major human pathogen responsible for superficial infections through to life-threatening invasive disease and the autoimmune sequelae acute rheumatic fever (ARF). Despite a significant global economic and health burden, there is no licensed vaccine available to prevent GAS disease. Several pre-clinical vaccines that target conserved GAS antigens are in development. ...
DOI:
10.1016/j.jim.2021.113194

18. Development and Evaluation of a Molecular Hepatitis A Virus Assay for Serum and Stool Specimens

Author:
Robert A. Kozak; Candace Rutherford; Melissa Richard-Greenblatt; N. Y. Elizabeth Chau; Ana Cabrera; Mia Biondi; Jamie Borlang; Jaqueline Day; Carla Osiowy; Sumathi Ramachandran; Nancy Mayer; Laurel Glaser; Marek Smieja
Source:
Viruses 2022 v.14 no.1 pp. -
ISSN:
1999-4915
Subject:
serology,   etc   ; Hepatovirus A; RNA; blood serum; diagnostic techniques; patients; public health; quantitative polymerase chain reaction; seroprevalence;     Show all 9 Subjects
Abstract:
... Hepatitis A virus (HAV) is an emerging public health concern and there is an urgent need for ways to rapidly identify cases so that outbreaks can be managed effectively. Conventional testing for HAV relies on anti-HAV IgM seropositivity. However, studies estimate that 10–30% of patients may not be diagnosed by serology. Molecular assays that can directly detect viral nucleic acids have the potenti ...
DOI:
10.3390/v14010159

19. Follow up of the Humoral Response in Healthcare Workers after the Administration of Two Dose of the Anti SARS-CoV-2 Vaccines—Effectiveness in Delta Variant Breakthrough Infections

Author:
Gema Fernández-Rivas; Jaume Barallat; Bibiana Quirant-Sánchez; Victoria González; María Doladé; Eva Martinez-Caceres; Monica Piña; Joan Matllo; Ignacio Blanco; Pere-Joan Cardona
Source:
Viruses 2022 v.14 no.7 pp. -
ISSN:
1999-4915
Subject:
serology,   etc   ; Severe acute respiratory syndrome coronavirus 2; antibodies; health services; humoral immunity; pandemic; summer; vaccination; Spain;     Show all 9 Subjects
Abstract:
... The implementation of vaccination among healthcare workers (HCWs) allowed the management of the pandemic in a manner that differed from that in the first waves. It has been demonstrated that the mRNA vaccines elicit good humoral responses but that there are still breakthrough infections. In summer 2021, a fifth wave emerged, despite the good coverage of HCWs in Spain. We aimed to study the SARS-Co ...
DOI:
10.3390/v14071385

20. Hepatitis E, Schistosomiasis and Echinococcosis–Prevalence in a Cohort of Pregnant Migrants in Germany and Their Influence on Fetal Growth Restriction

Author:
Janine Zöllkau; Juliane Ankert; Mathias W. Pletz; Sasmita Mishra; Gregor Seliger; Silvia M. Lobmaier; Clarissa U. Prazeres Da Costa; Vera Seidel; Katharina von Weizsäcker; Alexandra Jablonka; Christian Dopfer; Michael Baier; Thomas Horvatits; Ingrid Reiter-Owona; Tanja Groten; Benjamin T. Schleenvoigt
Source:
Pathogens 2022 v.11 no.1 pp. -
ISSN:
2076-0817
Subject:
serology,   etc   ; Orthohepevirus A; cohort studies; echinococcosis; fetal development; gestational age; hepatitis E; risk; schistosomiasis; seroprevalence; Germany;     Show all 11 Subjects
Abstract:
... Background: Infections, as well as adverse birth outcomes, may be more frequent in migrant women. Schistosomiasis, echinococcosis, and hepatitis E virus (HEV) seropositivity are associated with the adverse pregnancy outcomes of fetal growth restriction and premature delivery. Methods: A cohort study of 82 pregnant women with a history of migration and corresponding delivery of newborns in Germany ...
DOI:
10.3390/pathogens11010058