An official website of the United States government
Here’s how you know
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.
Shahnas M. Najimudeen; Catalina Barboza-Solis; Ahmed Ali; Sabrina M. Buharideen; Ishara M. Isham; Mohamed S.H. Hassan; Davor Ojkic; Guido Van Marle; Susan C. Cork; Frank van der Meer; Martine Boulianne; Mohamed Faizal Abdul-Careem
specificpathogen-freeanimals, etc ; Infectious bronchitis virus; chickens; histology; kidneys; lungs; pathogenesis; virology; Canada; Show all 9 Subjects
Abstract:
... The infectious bronchitis virus (IBV) 4/91 was one of the common IBV variants isolated in Eastern Canada between 2013 and 2017 from chicken flocks showing severe respiratory and production problems. We designed an in vivo experiment, using specific pathogen free (SPF) chickens, to study the pathogenesis of, and host response to, Canadian (CAN) 4/91 IBV infection. At one week of age, the chickens w ...
specificpathogen-freeanimals, etc ; Cherax quadricarinatus; aquaculture; eggs; eyes; formaldehyde; research; survival rate; water temperature; Show all 9 Subjects
Abstract:
... To solve the problems involved in the mass artificial incubation of redclaw crayfish eggs, we developed a recirculating mechanical pulling device to explore the technology of artificial incubation, including the egg stages, egg densities, attachments and incubator sizes. The eggs were separated and sterilized with 3000 ppm formaldehyde, and the water temperature was 28–29°C. The results were obtai ...
specificpathogen-freeanimals, etc ; Infectious bronchitis virus; air; histopathology; pathogenicity; poultry industry; viral load; viruses; China; Show all 9 Subjects
Abstract:
... Infectious bronchitis virus (IBV) has a significant impact on the poultry industry, and genogroup VI (GVI) IBVs, such as the TC07−2 strain, were reported in China since 2007. We isolated a novel strain, CK/CH/FJ/202005 (henceforth 202005), from broilers that were vaccinated with different attenuated IBV strains (H120, 4/91, or QX) in Fujian, China during 2020. Based on comparison with the SNU8065 ...
specificpathogen-freeanimals, etc ; Influenza A virus; chickens; cloaca; dogs; eggs; evolution; pathogenicity; seroconversion; viruses; China; Show all 11 Subjects
Abstract:
... At present, avian-origin H3N2 subtype canine influenza virus (H3N2 CIV) is prevalent in East Asian and North American countries. The host tropism of H3N2 CIV to mammals, including mice, guinea pigs, ferrets, and pigs, has been evaluated. However, it has not previously been determined whether avian-origin H3N2 CIV can be transmitted back to birds. In China, ducks, chickens, geese, pigeons, and quai ...
specificpathogen-freeanimals, etc ; Infectious bronchitis virus; chicken industry; collagen; cytokines; cytotoxicity; genotype; oviducts; pathogenicity; virology; Massachusetts; Show all 11 Subjects
Abstract:
... BACKGROUND: The QX-type infectious bronchitis virus (IBV) has become the predominant genotype worldwide in recent years and has caused serious economic losses to the chicken industry. The most significant feature of QX IBV is that its infection in the early growing stage can cause abnormal oviduct development, resulting in a high proportion of ‘false layers’ in poultry flocks of laying hens and br ...
... Nanovaccine is a revolutionary type of immunizations for various diseases that is simple to manufacture and administer. As a result, we are working to develop innovative nanovaccines against E. coli, which is capable of causing disease both inside and outside of its predilection sites, causing respiratory and systemic disease (colibacillosis).Colibacillosis is a global disease that significantly a ...
specificpathogen-freeanimals, etc ; Salmonella Braenderup; egg industry; eggs; human diseases; liver; nest boxes; oviducts; pollution; serotypes; spleen; Show all 11 Subjects
Abstract:
... In 2018, a national recall of shell eggs in the United States occurred due to human illness caused by Salmonella Braenderup. Although previous studies have identified Salmonella Braenderup in laying hens and the production environment, little is known about the ability of this Salmonella serovar to infect laying hens and contaminate eggs. The objective of this study was to examine the invasiveness ...
... Five novel chicken astrovirus (CAstV) strains, designated ZDF, MHC, WSC, WSW and MHW, were successfully isolated from chickens with gout, and were subjected to full genome sequencing characterization and tested for their pathogenic effects in specific pathogen-free (SPF) chicken embryos and chickens. The complete genomes of the five isolated strains were approximately 7436 nt to 7511 nt in length. ...
specificpathogen-freeanimals, etc ; Danio rerio; disinfection; equipment; fish; heterozygosity; homozygosity; mutants; parasites; pathogens; quality of life; spermatozoa; toxicology; Show all 13 Subjects
Abstract:
... Along with the growing number of laboratories that work with zebrafish (Danio rerio), it is necessary to have animals with good sanitary quality. Specific pathogens can interfere with the experimental results and in the life quality of the animals. Pseudoloma neurophilia is a parasite with high potential for interference in behavioural, morphology, toxicological and genetic research, and is very c ...
specificpathogen-freeanimals, etc ; Gallus gallus; Infectious bronchitis virus; RNA; White Leghorn; chickens; infectious bronchitis; viral load; virulence; viruses; Arkansas; Show all 11 Subjects
Abstract:
... Resistance to infectious bronchitis (IB) is a polygenic trait, but little is known about how resistance distributes in the host population. In this study, a relatively large number (n = 369) of specific-pathogen-free white leghorn chickens (Gallus gallus) were challenged with an Arkansas -type virulent IB virus (IBV), and resistance was evaluated 5 days after challenge by viral load (IBV RNA) in t ...
Thea Johnson; Kerri Jones; B. Tegner Jacobson; Julia Schearer; Noah Adams; Isaak Thornton; Cassie Mosdal; Steven Jones; Mark Jutila; Agnieszka Rynda-Apple; Thomas Besser; Diane Bimczok
... Mycoplasma ovipneumoniae (M. ovipneumoniae) is a respiratory pathogen associated with mild to moderate respiratory disease in domestic lambs and severe pneumonia outbreaks in wild ruminants such as bighorn sheep. However, whether M. ovipneumoniae by itself causes clinical respiratory disease in domestic sheep in the absence of secondary bacterial pathogens is still unclear. The goal of our study w ...
specificpathogen-freeanimals, etc ; DNA; Oncorhynchus mykiss; aquaculture; diagnostic techniques; droplets; etiological agents; farms; fish; histology; spleen; temperature; transmission electron microscopy; Show all 13 Subjects
Abstract:
... Red mark syndrome (RMS) is a skin disease of rainbow trout (Oncorhynchus mykiss), believed to be bacterial and caused by a Midichloria-like organism (MLO). Based on reports from farm sites, clinical signs of RMS are not observed above 15–16 °C. Here we describe the onset and progression of RMS at different temperatures under controlled experimental settings. Nine 180 L tanks at 12 °C, 15.5 °C and ...
specificpathogen-freeanimals, etc ; DNA; Protozoa; Toxoplasma gondii; anorexia; body temperature; chicks; immunology; microbiology; parasites; pathogenicity; spleen; tachyzoites; toxoplasmosis; weight gain; Show all 15 Subjects
Abstract:
... Toxoplasma gondii is an intracellular protozoan parasite that infects most warm-blooded animals, including humans, and causes toxoplasmosis. In this study, the pathogenicity of RH strain of T. gondii in broiler chicks was investigated and the susceptible tissues of chicks were clarified. Fourteen-day-old broiler chicks were injected intraperitoneally with 1×10⁷, 1×10⁶, and 1×10⁵ tachyzoites of T. ...
... Infectious bursal disease virus (IBDV), an Avibirnavirus, is the pathogen of infectious bursal disease, which is a severely immunosuppressive disease in 3–15‐week‐old chickens. Different phenotypes of IBDV, including classical, variant, very virulent (vv) and attenuated IBDV, have been reported in many chicken‐rearing countries worldwide. Here, we isolated and identified a naturally reassortant an ...
Oluwadamilola S. Omotainse; Nadeeka K. Wawegama; Sathya N. Kulappu Arachchige; Mauricio J. C. Coppo; Paola K. Vaz; Andrew P. Woodward; Somayeh Kordafshari; Mirjana Bogeski; Mark Stevenson; Amir H. Noormohammadi; Andrew W. Stent
specificpathogen-freeanimals, etc ; Mycoplasma synoviae; cell-mediated immunity; immunopathology; interleukin-17; light microscopy; live vaccines; macrophages; mucosa; respiratory tract diseases; vaccination; Show all 11 Subjects
Abstract:
... Mycoplasma synoviae causes respiratory tract disease in chickens characterised by mild to moderate lymphoplasmacytic infiltration of the tracheal mucosa. MS-H (Vaxsafe1 MS, Bioproperties Pty Ltd.) is an effective live attenuated vaccine for M. synoviae, but the immunological basis for its mechanism of protection has not been investigated, and the phenotypes of lymphocytes and associated cytokines ...
specificpathogen-freeanimals, etc ; African swine fever; blood serum; cross reaction; diagnostic sensitivity; diagnostic techniques; immunization; industry; live vaccines; serodiagnosis; swine; virulence; viruses; China; Show all 14 Subjects
Abstract:
... African swine fever (ASF) is a highly contagious hemorrhagic disease of pigs, posing a significant threat to the world pig industry. Several researchers are investigating the possibilities for developing a safe and efficient vaccine against ASF. In this regard, significant progress has been made and some gene-deleted ASFVs are reported as potential live attenuated vaccines. A seven-gene-deleted li ...
specificpathogen-freeanimals, etc ; Avian orthoavulavirus 1; adjuvants; antibodies; antibody formation; bursa of Fabricius; flagellin; flow cytometry; humoral immunity; immunophenotyping; microbiology; phosphates; spleen; vaccines; Show all 14 Subjects
Abstract:
... The aim of the present study was to evaluate the potential effect of flagellin as adjuvant in Newcastle disease virus (NDV) vaccine on the cellular and humoral immunity in chickens. Fifty-six specific pathogen-free chickens were assigned to seven groups of eight chickens and immunized twice with a two-week interval, intramuscularly. Group 1, received phosphate buffered saline as control (C), group ...
specificpathogen-freeanimals, etc ; Clostridium butyricum; Salmonella Enteritidis; cecum; chicks; drug resistance; epithelium; histopathology; hypoxia; interleukin-6; intestinal microorganisms; liver; poultry; probiotics; salmonellosis; spleen; Show all 16 Subjects
Abstract:
... Salmonellosis causes massive economic losses globally every year. Especially in poultry, numerous drug-resistant bacteria have emerged; thus, it is imperative to find alternatives to antibiotics. As a probiotic, Clostridium butyricum (C. butyricum) provides the latest strategy for inhibiting the proliferation of Salmonella. This study aimed to evaluate the effects of C. butyricum on intestinal env ...
... Avian hepatitis E virus (HEV) is the major causative pathogen of the big liver and spleen disease, hepatitis-splenomegaly syndrome, and hepatic rupture hemorrhage syndrome. Until now, there are 6 different avian HEV genotypes that infect chickens have been reported worldwide. Epidemiologic investigations of the avian HEV demonstrated that avian HEV has been widely spread in China in recent years. ...
specificpathogen-freeanimals, etc ; bioavailability; biodegradation; black tea; chemical degradation; circulatory system; depolymerization; flavanols; food chemistry; intestinal microorganisms; intestines; metabolism; metabolites; metabolomics; proanthocyanidins; theaflavins; urine; Show all 17 Subjects
Abstract:
... Thearubigins, polymers of tea catechins, account for more than 20% of the black tea polyphenols and have been reported to be the active components in black tea. However, the chemical structures and underlying mechanisms regarding how the thearubigins, being poorly bioavailable, generate in vivo health benefits are still largely unknown. Using germ-free and specific pathogen-free husbandry conditio ...