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1. A design strategy of dual-ratiomentric optical probe based on europium-doped carbon dots for colorimetric and fluorescent visual detection of anthrax biomarker

Author:
Zhou, Qiuju; Fang, Yajun; Li, Jiayu; Hong, Dan; Zhu, Panpan; Chen, Shihong; Tan, Kejun
Source:
Talanta 2021 v.222 pp. 121548
ISSN:
0039-9140
Subject:
spores,   etc   ; Bacillus subtilis; anthrax; biomarkers; carbon quantum dots; colorimetry; detection limit; fluorescence; humans; ligands; pyrolysis; urine;     Show all 12 Subjects
Abstract:
... In this work, a novel dual-ratiometric optical probe based on europium-doped carbon dots (Eu-CDs) was developed for colorimetric and fluorescent visual sensing dipicolinic acid (DPA), a anthrax biomarker. Eu-CDs with recessive signals were prepared via thermal pyrolysis method. And the magenta appears when Eu-CDs coordinates with Eriochrome Black T (EBT). Based on the ligand displacement strategy, ...
DOI:
10.1016/j.talanta.2020.121548

2. Microsporidian Enterocytozoon hepatopenaei (EHP) spores are inactivated in 1 min at 75 °C

Author:
Munkongwongsiri, Natthinee; Aldama-Cano, Diva January; Suebsing, Rungkarn; Thaiue, Dararat; Prasartset, Tharinthon; Itsathitphaisarn, Ornchuma; Sritunyalucksana, Kallaya
Source:
Aquaculture 2021 v.533 pp. 736178
ISSN:
0044-8486
Subject:
spores,   etc   ; DNA; Enterocytozoon; aquaculture feeds; extrusion; feed supplements; heat; in situ hybridization; manufacturing; polymerase chain reaction; shrimp; shrimp culture; tissues; viability;     Show all 14 Subjects
Abstract:
... The microsporidian Enterocytozoon hepatopenaei (EHP) is a major threat for shrimp culture. We hypothesized that commercial feeds processed at 75 °C or higher would present no risk for EHP transmission, even if they test positive for EHP by PCR. To test this hypothesis, the purified EHP spores (1 × 10⁶ spores) were incubated at 25 °C (non-heated) or 75 °C (heated) for 45–60 s prior to determining v ...
DOI:
10.1016/j.aquaculture.2020.736178

3. Life Cycles of Myxogastria Stemonitopsis typhina and Stemonitis fusca on Agar Culture

Author:
Dai, Dan; Okorley, Benjamin A.; Li, Yu; Zhang, Bo
Source:
Thejournal of eukaryotic microbiology 2020 v.67 no.1 pp. 66-75
ISSN:
1066-5234
Subject:
spore germination; spores,   etc   ; Myxogastrea; agar; fruiting bodies; morphogenesis; phylogeny; taxonomy;     Show all 8 Subjects
Abstract:
... Myxogastria is a group of protozoa characterized by cellular uninucleate amoeboflagellates (myxamoebae and flagellated swarm cell), acellular multinucleate plasmodia, and stationary spore‐bearing sporocarps. The Stemonitales is a large order in the Myxogastria and contains approximately 230 species, but only 13 species have their completed life cycles observed so far. Here, we described the life c ...
DOI:
10.1111/jeu.12754
PubMed:
31408563
PubMed Central:
PMC6973090

4. An Ultrastructural Study of the Extruded Polar Tube of Anncaliia algerae (Microsporidia)

Author:
Takvorian, Peter M.; Han, Bing; Cali, Ann; Rice, William J.; Gunther, Leslie; Macaluso, Frank; Weiss, Louis M.
Source:
Thejournal of eukaryotic microbiology 2020 v.67 no.1 pp. 28-44
ISSN:
1066-5234
Subject:
spores,   etc   ; Microsporidia; glycoproteins; microscopy; pathogenicity;     Show all 5 Subjects
Abstract:
... All microsporidia share a unique, extracellular spore stage, containing the infective sporoplasm and the apparatus for initiating infection. The polar filament/polar tube when exiting the spore transports the sporoplasm through it into a host cell. While universal, these structures and processes have been enigmatic. This study utilized several types of microscopy, describing and extending our unde ...
DOI:
10.1111/jeu.12751
PubMed:
31332877
PubMed Central:
PMC6944765

5. Rapid and sensitive colorimetric detection of microsporidian Enterocytozoon hepatopenaei (EHP) based on spore wall protein (SWP) gene using loop-mediated isothermal amplification combined with DNA functionalized gold nanoparticles as probes

Author:
Arunrut, Narong; Tondee, Benyatip; Khumwan, Pakapreud; Kampeera, Jantana; Kiatpathomchai, Wansika
Source:
Aquaculture 2021 v.533 pp. 736206
ISSN:
0044-8486
Subject:
spores,   etc   ; Enterocytozoon; colorimetry; cross reaction; detection limit; electrophoresis; farms; fluorometry; genes; growth retardation; hatcheries; laboratory equipment; loop-mediated isothermal amplification; nanogold; nucleic acid hybridization; pathogens; plasmids; polymerase chain reaction; rapid methods; ribosomal RNA; shrimp;     Show all 21 Subjects
Abstract:
... Enterocytozoon hepatopenaei (EHP) is a newly emerging microsporidian parasite that causes growth retardation in cultured shrimp. Currently, EHP infection can be detected by standard polymerase chain reaction (PCR)-based methods, as well as loop-mediated isothermal amplification (LAMP) methods that target EHP's small subunit (SSU) ribosomal RNA (rRNA) gene (LAMP-SSU). However, this gene target has ...
DOI:
10.1016/j.aquaculture.2020.736206

6. Mycorrhizal inoculation impact on Acorus calamus L. - An ethnomedicinal plant of western Himalaya and its in silico studies for anti-inflammatory potential

Author:
Verma, Rachna; Kumar, Dinesh; Nagraik, Rupak; Sharma, Avinash; Tapwal, Ashwani; Puri, Sunil; Kumar, Harsh; Kumari, Amita; Nepovimova, Eugenie; Kuca, Kamil
Source:
Journal of ethnopharmacology 2021 v.265 pp. 113353
ISSN:
0378-8741
Subject:
spores,   etc   ; 2,2-diphenyl-1-picrylhydrazyl; Acorus calamus; Glomus mosseae; anti-inflammatory activity; antimicrobial properties; antioxidant activity; antioxidants; chemical constituents of plants; computer simulation; energy; gas chromatography-mass spectrometry; mineral fertilizers; phytochemicals; plant extracts; receptors; rhizomes; traditional medicine; Himalayan region;     Show all 19 Subjects
Abstract:
... Different plants are used for the treatment of various ailments and Acorus calamus L. is one such plant found in Western Himalaya. Rhizome of this plants has ethnomedicinal significance, as its rhizome is used for curing fever, pain and inflammation. An attempt has been made to alter the phytochemicals and increase its antioxidant property in a sustainable way with the help of mycorrhizal inoculat ...
DOI:
10.1016/j.jep.2020.113353

7. Spores re-suspending technology, a new system improving spore seeding for culture of commercial red seaweeds

Author:
Pérez Massad, Ignacio; Ávila, Marcela; Contreras-Porcia, Loretto; Bulboa Contador, Cristian
Source:
Aquaculture 2020 v.526 pp. 735374
ISSN:
0044-8486
Subject:
spores,   etc   ; Porphyra; germination; models; tanks; thallus;     Show all 6 Subjects
Abstract:
... In the present study we introduce the development and implementation of a new technology for improving the seeding in the spore-based cultures on red algae (excluding Porphyra), using a spore re-suspending technology (SRT). Such technology has two operation differences with the current methods: (i) it allows the use of vertically placed frames, and (ii) resuspends the spores not yet settled from t ...
DOI:
10.1016/j.aquaculture.2020.735374

8. Probiotic: conceptualization from a new approach

Author:
Zendeboodi, Fatemeh; Khorshidian, Nasim; Mortazavian, Amir M; da Cruz, Adriano G
Source:
Current opinion in food science 2020 v.32 pp. 103-123
ISSN:
2214-7993
Subject:
spores,   etc   ; food science; metabolites; probiotics; terminology;     Show all 5 Subjects
Abstract:
... According to the proposed definition by FAO/WHO, probiotics must be alive and abundant once ingested. However, during recent years, new definitions are added to the probiotic terminology such as ‘paraprobiotics’ (dead/inactive cells of probiotics) and ‘postbiotics’ (healthful metabolites of probiotics), because findings have shown that dead cells (intact or ruptured) could also show significant he ...
DOI:
10.1016/j.cofs.2020.03.009

9. Live cell imaging of cell movement and transdifferentiation during regeneration of an amputated multicellular body of the social amoeba Dictyostelium discoideum

Author:
Mohri, Kurato; Tanaka, Ryodai; Nagano, Seido
Source:
Developmental biology 2020 v.457 no.1 pp. 140-149
ISSN:
0012-1606
Subject:
spores,   etc   ; Dictyostelium discoideum; cell movement; image analysis;     Show all 4 Subjects
Abstract:
... The regeneration of lost body parts is a fascinating phenomenon exhibited by some multicellular organisms. In social amoebae, such as Dictyostelium discoideum, the pseudoplasmodium is a temporary migratory multicellular structure with high regeneration ability. It consists of future stalk cells (prestalk cells) at the anterior end and future spore cells (prespore cells) at the posterior end, and i ...
DOI:
10.1016/j.ydbio.2019.09.014

10. A Revised Understanding of Clostridioides difficile Spore Germination

Author:
Lawler, Amelia J.; Lambert, Peter A.; Worthington, Tony
Source:
Trends in microbiology 2020 v.28 no.9 pp. 744-752
ISSN:
0966-842X
Subject:
spore germination; spores,   etc   ; Clostridium difficile; amino acids; bile salts; cortex; models; peptidoglycans; serine proteinases;     Show all 9 Subjects
Abstract:
... The dormant resistant spores of Clostridioides difficile are transformed into metabolically active cells through the process of germination. Spore germination in C. difficile is regulated by the detection of bile salt germinants and amino acid cogerminants by pseudoproteases CspC and CspA, respectively. The germinant signal is transduced to the serine protease CspB, which processes the cortex lyti ...
DOI:
10.1016/j.tim.2020.03.004

11. Bacillus spore awakening: recent discoveries and technological developments

Author:
Xing, Yun; Harper, Willie F
Source:
Current opinion in biotechnology 2020 v.64 pp. 110-115
ISSN:
0958-1669
Subject:
bacterial spores; spore germination,   etc   ; Bacillus (bacteria); engineering; metabolomics; mining; protein synthesis;     Show all 7 Subjects
Abstract:
... Elucidating the mechanistic basis of spore germination and outgrowth remains a difficult challenge with crucial implications in the numerous scientific and engineering disciplines. Omics studies have revealed numerous new biochemical insights and single-spore studies link molecular insights directly with spore heterogeneity behaviors. This field is expected to advance at greater speed with the app ...
DOI:
10.1016/j.copbio.2019.12.024

12. Ethanol treatment does not inactivate spore-forming bacteria – A cautionary note about the safe transport of bacteria prior to identification by MALDI-TOF MS

Author:
Palombo, Enzo A.
Source:
Journal of microbiological methods 2020 v.172 pp. 105893
ISSN:
0167-7012
Subject:
bacterial spores; spore-forming bacteria,   etc   ; ethanol; matrix-assisted laser desorption-ionization mass spectrometry;     Show all 4 Subjects
Abstract:
... Treatment with 70% ethanol has been proposed as a safe and effective way to inactive bacteria for transport between laboratories prior to identification by MALDI-TOF MS. Ethanol alone does not inactivate spore-forming bacteria and additional chemical or physical treatment is necessary to guarantee inactivation of bacterial spores. ...
DOI:
10.1016/j.mimet.2020.105893

13. Comparison between two kinds of natural pollen traps in tropical China: ants’ nests on tree branches versus surface soil

Author:
Zhang, Zhi-Yong; Yang, Feng-Chun; Cheng, Dong-Mei; Ferguson, David K.; Hu, Wan; Li, Yue-Zhuo; Li, Cheng-Sen
Source:
Palynology 2020 v.44 no.1 pp. 4-11
ISSN:
1558-9188
Subject:
spore traps; spores,   etc   ; Formicidae; ant nests; botanical gardens; branches; palynology; pollen; soil; soil sampling; China;     Show all 11 Subjects
Abstract:
... Natural pollen traps have been known for many years. This study analyzed the palynological characteristics of 12 ant nests on branches and 12 surface soil samples, collected from tropical areas of Yunnan and Hainan in China. Pollen grains and spores of 21 taxa belonging to 21 families were recorded from Xishuangbanna Tropical Botanical Garden and 30 taxa from 27 families were recorded from Xinglon ...
DOI:
10.1080/01916122.2018.1549117

14. Combining spore germination and heat inactivation to decontaminate materials contaminated with Bacillus anthracis spores

Author:
Buhr, T.L.; Minter, Z.A.; Kennihan, N.L.; Young, A.A.; Borgers‐Klonkowski, E.L.; Osborn, E.B.; Bohmke, M.D.; Hamilton, S.M.; Kimani, M.B.; Miller, C.T.; Mackie, R.S.; Innocenti, J.M.; Bensman, M.D.; Lilly, S.D.
Source:
Journal of applied microbiology 2020 v.128 no.1 pp. 124-137
ISSN:
1364-5072
Subject:
spore germination; spores,   etc   ; Bacillus anthracis; air; aircraft; coatings; decontamination; heat inactivation; industry; nylon; temperature;     Show all 11 Subjects
Abstract:
... AIMS: To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75–80°C, ≥72 h, 70–90% RH, down to ≤60°C and ≤24 h total decontamination time. METHODS AND RESULTS: Bacillus anthracis spore germination with l‐alanine+inosine+calcium dipicolinate (CaDPA) was quantified at 0–40°C, s ...
DOI:
10.1111/jam.14474

15. Colletotrichum asianum causes anthracnose in Philippine mango cv. Carabao

Author:
Alvarez, Lourdes V.; Hattori, Yukako; Deocaris, Chester C.; Mapanao, Carmelita P.; Bautista, Arcibel B.; Cano, Mark Joseph B.; Naito, Kouske; Kitabata, Shunsuke; Motohashi, Keiichi; Nakashima, Chiharu
Source:
Australasian plant disease notes 2020 v.15 no.1 pp. 13
ISSN:
1833-928X
Subject:
spores,   etc   ; Colletotrichum; anthracnose; fruits; fungi; genes; leaves; mangoes; Philippines;     Show all 9 Subjects
Abstract:
... Anthracnose symptoms were observed on fruits and leaves of mango cv. Carabao at several locations in the Philippines. Single spore isolates were generated from affected leaves and fruits and identified as Colletotrichum asianum based on morphology and molecular analyses using several genes. Inoculation of C. asianum isolates onto healthy fruit of mango cv. Carabao resulted in the same symptoms as ...
DOI:
10.1007/s13314-020-00384-x

16. Effects of food availability on microsporidiosis and alkaloid production in the two-spotted lady beetle, Adalia bipunctata L.

Author:
Steele, T.; Singer, R.D.; Bjørnson, S.
Source:
Journal of invertebrate pathology 2020 v.175 pp. 107443
ISSN:
0022-2011
Subject:
spores,   etc   ; Adalia bipunctata; Nosema; alkaloids; food availability; microbial load; microsporidiosis;     Show all 7 Subjects
Abstract:
... The effects of food availability and infection with the microsporidium Nosema adaliae on alkaloid production in the two-spotted lady beetle, Adalia bipunctata L., was examined. Alkaloid production (relative percent adaline in reflex-fluid) and pathogen load (spore counts) were quantified for both uninfected and N. adaliae-infected A. bipunctata. Alkaloid content was significantly higher for beetle ...
DOI:
10.1016/j.jip.2020.107443

17. Of birches, smoke and reindeer dung – Tracing human-environmental interactions palynologically in sediments from the Nahe palaeolake

Author:
Krüger, Sascha
Source:
Journal of archaeological science: Reports 2020 v.32 pp. 102370
ISSN:
2352-409X
Subject:
fungal spores,   etc   ; anthropogenic activities; archaeology; charcoal; palynology; sediments; vegetation; Germany;     Show all 8 Subjects
Abstract:
... Sediments from the Nahe palaeolake in Schleswig-Holstein provided a continuous and high-resolution palynological record in the vicinity of a Lateglacial archaeological site in northern Germany. The principal objective of the study was to investigate potential reflections of Palaeolithic human-environmental interaction, which is generally regarded as challenging. Charcoal particle frequencies in di ...
DOI:
10.1016/j.jasrep.2020.102370

18. A rapid rice blast detection and identification method based on crop disease spores' diffraction fingerprint texture

Author:
Yang, Ning; Yu, Junjie; Wang, Aiying; Tang, Jian; Zhang, Rongbiao; Xie, Liangliang; Shu, Fangyu; Kwabena, Oppong Paul
Source:
Journal of the science of food and agriculture 2020 v.100 no.9 pp. 3608-3621
ISSN:
0022-5142
Subject:
spores,   etc   ; blast disease; fungi; models; paddies; rice; texture; China;     Show all 8 Subjects
Abstract:
... BACKGROUND: Rice blast fungus is a worldwide disease, and it is one of the most serious rice diseases in the north and south rice fields in China. The initial symptoms of rice blast are not obvious, and the speed of transmission is fast. Manual identification is time‐consuming and laborious. At present, it is a great challenge to realize rapid and accurate early identification of rice blast. RESUL ...
DOI:
10.1002/jsfa.10383

19. All’s well? Comparing on- and off-site pollen samples and exploring the potential of pollen from man-made contexts

Author:
van Amerongen, Yvonne F.
Source:
Vegetation history and archaeobotany 2020 v.29 no.2 pp. 125-131
ISSN:
0939-6314
Subject:
spore traps,   etc   ; forests; pollen; pollen analysis; pollen sampling; wells; Netherlands;     Show all 7 Subjects
Abstract:
... Pollen analysis has long been used as a tool to make an assessment of regional vegetation. On-site pollen samples are taken for the same purpose at some excavations, because they are often the only available contexts with good preservation conditions. This paper shows that the analysis of on- and off-site pollen samples from the same region (West Frisia, the Netherlands) and time period (Dutch Bro ...
DOI:
10.1007/s00334-019-00759-w

20. c-di-GMP Arms an Anti-σ to Control Progression of Multicellular Differentiation in Streptomyces

Author:
Gallagher, Kelley A.; Schumacher, Maria A.; Bush, Matthew J.; Bibb, Maureen J.; Chandra, Govind; Holmes, Neil A.; Zeng, Wenjie; Henderson, Max; Zhang, Hengshan; Findlay, Kim C.; Brennan, Richard G.; Buttner, Mark J.
Source:
Molecular cell 2020 v.77 no.3 pp. 586-599.e6
ISSN:
1097-2765
Subject:
spores,   etc   ; Streptomyces; antibiotics; hyphae; sigma factors; sporulation; vegetative growth;     Show all 7 Subjects
Abstract:
... Streptomyces are our primary source of antibiotics, produced concomitantly with the transition from vegetative growth to sporulation in a complex developmental life cycle. We previously showed that the signaling molecule c-di-GMP binds BldD, a master repressor, to control initiation of development. Here we demonstrate that c-di-GMP also intervenes later in development to control differentiation of ...
DOI:
10.1016/j.molcel.2019.11.006
PubMed:
31810759
PubMed Central:
PMC7005675