PubAg

Main content area

You searched for: Subject "staining" Remove constraint Subject: "staining"
Start Over

Search

7,995  Search Results

« Previous | 1 - 20 of 7,995 |
Number of results to display per page

Search Results

1. Revealing a decades-old fingermark with cyanoacrylate fuming and rhodamine 6G

Author:
Tapps, Maralee; McMullen, Laurence; Gagné, Marie-Eve; Beaudoin, Alexandre
Source:
Forensic science international 2019 v.300 pp. e9
ISSN:
0379-0738
Subject:
staining,   etc   ; crime; forensic sciences; lasers; rhodamines;     Show all 5 Subjects
Abstract:
... Cyanoacrylate fuming, rhodamine 6G staining and 532 nm laser light visualization were used to reveal a thirty-year-old fingermark on a plastic bag discovered at a double homicide scene. ...
DOI:
10.1016/j.forsciint.2019.04.025

2. Single metal deposition versus physical developer: A comparison between two advanced fingermark detection techniques

Author:
Moret, Sébastien; Lee, Po Lun Timothy; de la Hunty, Mackenzie; Spindler, Xanthe; Lennard, Chris; Roux, Claude
Source:
Forensic science international 2019 v.294 pp. 103-112
ISSN:
0379-0738
Subject:
staining,   etc   ; amino acids; gold; silver; zinc;     Show all 5 Subjects
Abstract:
... Single metal deposition (SMD II) is a fingermark detection technique based on the use of colloidal gold. The technique has been simplified and optimised over the years to become more reliable, sensitive and user-friendly. Physical developer (PD) is a well-established detection method based on silver deposition from a redox solution. This study presents an extensive comparison of SMD II against PD ...
DOI:
10.1016/j.forsciint.2018.10.032

3. Investigation of some of the factors influencing fingermark detection

Author:
Chadwick, Scott; Moret, Sebastien; Jayashanka, Nilesh; Lennard, Chris; Spindler, Xanthe; Roux, Claude
Source:
Forensic science international 2018 v.289 pp. 381-389
ISSN:
0379-0738
Subject:
staining,   etc   ; rhodamines; United Kingdom;     Show all 3 Subjects
Abstract:
... The primary aims of fingermark detection research are to improve the quality and increase the rate of detection of identifiable impressions. This is usually performed through the development of new methods and technologies to provide alternatives to or improve current procedures. While research of this nature is important to pursue, it fails to address the underlying question related to the factor ...
DOI:
10.1016/j.forsciint.2018.06.014

4. In Situ Partial Treatment of Single Cells by Laminar Flow in the “Open Space”

Author:
Zhang, Qiang; Mao, Sifeng; Khan, Mashooq; Feng, Shuo; Zhang, Wanling; Li, Weiwei; Lin, Jin-Ming
Source:
Analytical chemistry 2018 v.91 no.2 pp. 1644-1650
ISSN:
1520-6882
Subject:
staining,   etc   ; fluorescein; laminar flow; solutes;     Show all 4 Subjects
Abstract:
... Regional difference of a single cell is nonignorable, which means it is not precise to investigate the single cell as a homogeneous object. A convenient method to investigate cellular response to the treatment at the subcellular level is still needed. In this work, we developed a microfluidic approach for manipulating a partial region of a single adherent cell by generating a stable distribution o ...
DOI:
10.1021/acs.analchem.8b05313

5. Apelin-13 attenuates motor impairments and prevents the changes in synaptic plasticity-related molecules in the striatum of Parkinsonism rats

Author:
Haghparast, Elham; Sheibani, Vahid; Abbasnejad, Mehdi; Esmaeili-Mahani, Saeed
Source:
Peptides 2019 v.117 pp. 170091
ISSN:
0196-9781
Subject:
staining,   etc   ; Parkinson disease; glutamate receptors; immunoblotting; immunohistochemistry; memory; neurons; rats;     Show all 8 Subjects
Abstract:
... The striatum plays a critical role in motor control and also learning and memory of motor skills. It has been reported that striatal synaptic components are significantly decreased in dopaminergic-denervated striatum. In this study the effects of apelin-13 were investigated on motor disorders and striatal synaptosomal expression of PSD-95, neurexin1, neuroligin, metabotropic glutamate receptor (mG ...
DOI:
10.1016/j.peptides.2019.05.003

6. Description of an ultrasound-guided erector spinae plane block and the spread of dye in dog cadavers

Author:
Ferreira, Tatiana H.; St James, Mariko; Schroeder, Carrie A.; Hershberger-Braker, Karen L.; Teixeira, Leandro B.C.; Schroeder, Kristopher M.
Source:
Veterinary anaesthesia and analgesia 2019 v.46 no.4 pp. 516-522
ISSN:
1467-2987
Subject:
staining,   etc   ; dogs; methylene blue; muscles; nerve tissue; ultrasonography;     Show all 6 Subjects
Abstract:
... To describe a technique to perform an ultrasound-guided erector spinae plane (ESP) block and determine the distribution and potential complications after injection of two volumes of methylene blue in dog cadavers.Prospective experimental cadaveric study.A total of eight dog cadavers weighing 9.3 ± 1.9 kg.Ultrasound-guided injections dorsal to the transverse process and ventral to the erector spina ...
DOI:
10.1016/j.vaa.2019.03.002

7. In-plane and out-of-plane needle insertion comparison for a novel lateral block of the radial, ulnar, median and musculocutaneous nerves in cats

Author:
Leung, Jessica B.Y.; Rodrigo-Mocholi, Diego; Martinez-Taboada, Fernando
Source:
Veterinary anaesthesia and analgesia 2019 v.46 no.4 pp. 523-528
ISSN:
1467-2987
Subject:
staining,   etc   ; cats; methylene blue; muscles; nerve tissue; ultrasonics;     Show all 6 Subjects
Abstract:
... To compare two needle insertion techniques in a novel lateral approach to the radial, ulnar, median and musculocutaneous (RUMM) nerve block in cat cadavers.Prospective, cadaveric experimental study.A group of 18 feline cadavers.Cadavers were divided into two groups. Both thoracic limbs of each cat were ‘blocked’ using the ‘in-plane’ (IP) or ‘out-of-plane’ (OP) ultrasound (US)-guided method. A sing ...
DOI:
10.1016/j.vaa.2019.02.006

8. Alpha-enolase staining patterns in the renal tissues of cats with and without chronic kidney disease

Author:
McLeland, Shannon; Quimby, Jessica; Lappin, Michael R.
Source:
Veterinary immunology and immunopathology 2019 v.212 pp. 23-26
ISSN:
0165-2427
Subject:
staining,   etc   ; cats; epithelium; histopathology; immunohistochemistry; nephritis; pathogenesis; phosphopyruvate hydratase;     Show all 8 Subjects
Abstract:
... Renal α-enolase has variable expression in inflammatory and neoplastic diseases. Therefore, in order to define the distribution of α-enolase in renal tissues of cats, an immunohistochemistry assay was validated and described here. Tissues from 29 cats with IRIS Stage 2–4 CKD, 8 control cats < 2 years of age, and 4 control cats> 10 years of age were assessed. Interstitial nephritis was the predomin ...
DOI:
10.1016/j.vetimm.2019.04.009

9. Repair of dentine-related lesions without a drill or injection

Author:
Zhang, Yu Yuan; Wong, Hai Ming; McGrath, Colman P. J.; Li, Quan Li
Source:
RSC advances 2019 v.9 no.26 pp. 15099-15107
ISSN:
2046-2069
Subject:
staining,   etc   ; electric field; in vivo studies; rabbits; teeth;     Show all 5 Subjects
Abstract:
... For covering the shortages of traditional treatments, a novel and non-invasive system was developed with the simple adaption of nature's own repair process, while an extrinsic electric field was introduced to improve its remineralization kinetics. In an in vivo study, acid-etched rabbit dentine was used to evaluate the remineralization efficacy and safety of the system. The exposed dentine tubules ...
DOI:
10.1039/c9ra02759a

10. Immobilization of plasmids in bacterial nanocellulose as gene activated matrix

Author:
Pötzinger, Yvette; Rahnfeld, Lisa; Kralisch, Dana; Fischer, Dagmar
Source:
Carbohydrate polymers 2019 v.209 pp. 62-73
ISSN:
0144-8617
Subject:
staining,   etc   ; bacteria; biocompatibility; cellulose; genes; plasmids; scanning electron microscopy; transfection;     Show all 8 Subjects
Abstract:
... The synergy of the local delivery of nucleic acids using a hydrogel-based gene activated matrix (GAM) might support regenerative processes on a genetic level by concurrently providing a cell-friendly microenvironment. To investigate bacterial nanocellulose (BNC) as GAM, two plasmids (pSV-β-Gal and pGL3) were incorporated by reswelling and injection techniques forming matrix and core-shell systems ...
DOI:
10.1016/j.carbpol.2019.01.009

11. Cryopreservation of cell line Paesun by a rapid cooling

Author:
Song, Jong Kui; Hui, Yu Lan
Source:
Cryobiology 2019 v.87 pp. 115-116
ISSN:
0011-2240
Subject:
staining,   etc   ; cell lines; cooling; cryopreservation; liquids; nitrogen; thawing; vapors; viruses;     Show all 9 Subjects
Abstract:
... The purpose of the present study was to clarify the possibility of a rapid cryopreservation for cell line Paesun by cooling in the range of 30–40 °C/min to vapor phase of −120 ∼-140 °C before immersion into liquid phase of liquid nitrogen using 10% Me2SO. After thawing, these cells were examined with assaying viability by trypan blue exclusion staining and survival by cloning in monolayer; the per ...
DOI:
10.1016/j.cryobiol.2019.01.017

12. A valve‐based microfluidic device for on‐chip single cell treatments

Author:
Sun, Yue; Cai, Bo; Wei, Xiaoyun; Wang, Zixiang; Rao, Lang; Meng, Qian‐Fang; Liao, Qingquan; Liu, Wei; Guo, Shishang; Zhao, Xingzhong
Source:
Electrophoresis 2019 v.40 no.6 pp. 961-968
ISSN:
0173-0835
Subject:
staining,   etc   ; bioassays; biomedical research; calcium; droplets; electrophoresis; hydrogels; sodium alginate;     Show all 8 Subjects
Abstract:
... Assays toward single‐cell analysis have attracted the attention in biological and biomedical researches to reveal cellular mechanisms as well as heterogeneity. Yet nowadays microfluidic devices for single‐cell analysis have several drawbacks: some would cause cell damage due to the hydraulic forces directly acting on cells, while others could not implement biological assays since they could not im ...
DOI:
10.1002/elps.201800213

13. DNA recovery from gelatin fingerprint lifters by direct proteolytic digestion

Author:
Zieger, Martin; Schneider, Christoph; Utz, Silvia
Source:
Forensic science international 2019 v.295 pp. 145-149
ISSN:
0379-0738
Subject:
staining,   etc   ; DNA; DNA fingerprinting; forensic sciences; gelatin; magnetism; proteolysis; protocols;     Show all 8 Subjects
Abstract:
... Fingerprints are a valuable source for DNA profiling in forensic investigations. In practice, the fingerprints are routinely visualized first by powder staining and then often transferred to tapes or gelatin lifters for storage or examination. If at all, fingerprints are usually sampled for DNA in a second step. To target the DNA sampling in an optimal way, it is essential to know how much of the ...
DOI:
10.1016/j.forsciint.2018.12.006

14. Fast and sensitive fluorescent probe for ratiometric detection of hydrogen sulfide in mitochondria

Author:
Jiang, Na; Wang, Bin; Liu, Tao; Liu, Qingyu; Wei, Qin; Xing, Yujing; Zheng, Gengxiu
Source:
Analytical methods 2019 v.11 no.2 pp. 232-235
ISSN:
1759-9679
Subject:
staining,   etc   ; analytical methods; fluorescent dyes; hydrogen sulfide; mitochondria;     Show all 5 Subjects
Abstract:
... A probe Mito-BT was constructed and synthesized by combining triphenylamine and hemicyanine, and can be used for specific ratiometric sensing of H₂S. It has a rapid response time of 10 s and preferentially targets to mitochondria. Then the visualization of the change of the mitochondrial H₂S concentration upon NaHS incubation by using Mito-BT staining is promising for the practical exploration of ...
DOI:
10.1039/c8ay02586j

15. An accessible and high-throughput strategy of continuously monitoring apoptosis by fluorescent detection of caspase activation

Author:
Hanson, Kelly M.; Finkelstein, Jacob N.
Source:
Analytical biochemistry 2019 v.564-565 pp. 96-101
ISSN:
0003-2697
Subject:
staining,   etc   ; apoptosis; automation; caspases; cost effectiveness; dose response; fluorescence; monitoring;     Show all 8 Subjects
Abstract:
... We present a real-time, high-throughput, and cost-effective method of detecting apoptosis in vitro using a previously developed reagent that detects caspase activation by fluorescence. Current methods of assessing apoptosis fail to account for the dimension of time, and thus are limited in data yielded per sample. This reagent allows real-time detection of apoptosis, but until now has been restric ...
DOI:
10.1016/j.ab.2018.10.022

16. Reference limits for hepatic bile duct-to-arteriole and bile duct-to-portal tract ratios in healthy cats

Author:
Chandler, Alyssa M.; Center, Sharon A.; Randolph, John F.; Davignon, Danielle L.; McDonough, Sean P.; Warner, Karen L.
Source:
American journal of veterinary research 2019 v.80 no.1 pp. 15-23
ISSN:
0002-9645
Subject:
staining,   etc   ; arterioles; bile; bile ducts; biopsy; cats; immunohistochemistry; morphometry;     Show all 8 Subjects
Abstract:
... OBJECTIVE To establish reference limits for hepatic bile duct-to-arteriole ratio (BD:A) and bile duct-to-portal tract ratio (BD:PT) in healthy cats and assess whether these parameters could be used to support a diagnosis of biliary ductopenia in cats. SAMPLE Hepatic biopsy samples from healthy cats (n = 20) and cats with ductopenia (2). PROCEDURES Hepatic biopsy samples from healthy cats were used ...
DOI:
10.2460/ajvr.80.1.15

17. Assessment of the potential efficacy of blind perineural injection techniques for blockade of the saphenous, obturator, and lateral cutaneous femoral nerves in dog cadavers

Author:
Echeverry-Bonilla, Diego F.; Pelaez, Jessica T.; Buritica, Edwin F.; Laredo, Francisco G.
Source:
American journal of veterinary research 2017 v.78 no.4 pp. 412-420
ISSN:
0002-9645
Subject:
staining,   etc   ; dogs; nerve tissue;     Show all 3 Subjects
Abstract:
... OBJECTIVE To evaluate the potential efficacy of blind perineural injection techniques to provide nerve blockade of the saphenous, obturator, and lateral cutaneous femoral nerves by assessing the distribution along those nerves of 3 volumes of an injected staining solution in dog cadavers. ANIMALS 18 canine cadavers. PROCEDURES The anatomic characteristics of the target nerves were evaluated by dis ...
DOI:
10.2460/ajvr.78.4.412

18. An Optimized Protocol of Protargol Staining for Ciliated Protozoa

Author:
Ji, Daode; Wang, Yunfeng
Source:
Thejournal of eukaryotic microbiology 2018 v.65 no.5 pp. 705-708
ISSN:
1066-5234
Subject:
staining,   etc   ; Ciliophora; Protozoa; bleaching; species identification;     Show all 5 Subjects
Abstract:
... Protargol staining is a crucial method to reveal the infraciliature of ciliates, which is the most important morphological character for species identification. In the present study, Wilbert's protocol of protargol staining was emended mainly toward the highly happened improper bleaching. Through reciprocal treatments, both insufficient and excessive bleachings were much eliminated from the protar ...
DOI:
10.1111/jeu.12515

19. Label-Free, Direct Measurement of Protein Concentrations in Turbid Solutions with a UV–Visible Integrating Cavity Absorbance Spectrometer

Author:
Larson, Nicholas R.; Wei, Yangjie; Middaugh, C. Russell
Source:
Analytical chemistry 2018 v.90 no.8 pp. 4982-4986
ISSN:
1520-6882
Subject:
staining,   etc   ; absorbance; biotechnology; microparticles; nanogold; proteins; spectrometers;     Show all 7 Subjects
Abstract:
... Protein–particle conjugates and mixtures have been investigated extensively for their diverse applications in biotechnology. However, general methods to measure protein concentration of protein–particle solutions are lacking. Typically, proteins in turbid solutions require separation or staining with another chromophore to quantitate their concentration. Here we demonstrate a label-free, direct ap ...
DOI:
10.1021/acs.analchem.8b00502

20. Compact in-line lensfree digital holographic microscope

Author:
Rostykus, Manon; Soulez, Ferréol; Unser, Michael; Moser, Christophe
Source:
Methods 2018 v.136 pp. 17-23
ISSN:
1046-2023
Subject:
staining,   etc   ; image analysis; lighting; microscopes; microscopy;     Show all 5 Subjects
Abstract:
... Phase imaging provides intensity contrast to visualize transparent samples such as found in biology without any staining. Among them, digital holographic microscopy (DHM) is a well-known quantitative phase method. Lensfree implementations of DHMs offer the added advantage to provide large field of views (several mm2 compared to several hundred μm2) and more compact setups that traditional DHM whic ...
DOI:
10.1016/j.ymeth.2017.11.008