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1. Analysis of microplastics in wetland samples from coastal Ghana using the Rose Bengal stain

Author:
Gbogbo, Francis; Takyi, James Benjamin; Billah, Maxwell Kelvin; Ewool, Julliet
Source:
Environmental monitoring and assessment 2020 v.192 no.4 pp. 208
ISSN:
0167-6369
Subject:
staining,   etc   ; color; microplastics; sediments; wetlands; Ghana;     Show all 6 Subjects
Abstract:
... The use of optical microscope remains the most commonly used technique for microplastic identification and quantification despite major limitations with misidentifications and biases. We evaluated the use of the 1% Rose Bengal stain in improving the identification of microplastics after a standard microplastic isolation process. The stain discriminated organic materials from potential microplastic ...
DOI:
10.1007/s10661-020-8175-8

2. Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Author:
Andraszek, Katarzyna; Banaszewska, Dorota; Szeleszczuk, Olga; Niedbała, Piotr; Kuchta‐Gładysz, Marta
Source:
Reproduction in domestic animals 2020 v.55 no.4 pp. 515-522
ISSN:
0936-6768
Subject:
silver staining,   etc   ; acrosome; epididymis; semen; silver nitrate;     Show all 5 Subjects
Abstract:
... Sperm cells isolated from the tail of the epididymis and from the semen of the same individuals were analysed. The use of silver nitrate to stain sperm cells isolated from the tail of the epididymis made it possible to identify structures that were not visible in the sperm from semen. Silver nitrate very clearly distinguished the acrosomal and distal parts of the sperm head. Following silver nitra ...
DOI:
10.1111/rda.13646

3. The dead cell ratio of bacteria in sludge flocs as an indicator of sludge reduction in sludge ozone process

Author:
Hashimoto, Kurumi; Marushima, Toru; Nakai, Satoshi; Nishijima, Wataru; Motoshige, Hiroshi
Source:
Biochemical engineering journal 2020 v.154 pp. 107427
ISSN:
1369-703X
Subject:
staining,   etc   ; analytical methods; death; ozone; propidium; sludge;     Show all 6 Subjects
Abstract:
... A quantitative analytical method to determine the dead cell ratio of bacteria (death ratio) in sludge was developed for evaluating technologies and determining a sufficient amount of ozone for the sludge ozone process. The method with SYTO9 and propidium iodide as staining agents for live and dead cells, respectively, was optimized. The death ratio developed in this study could successfully predic ...
DOI:
10.1016/j.bej.2019.107427

4. Major factors influencing the quantification of Nile Red stained microplastics and improved automatic quantification (MP-VAT 2.0)

Author:
Prata, Joana C.; Alves, Jorge R.; da Costa, João P.; Duarte, Armando C.; Rocha-Santos, Teresa
Source:
The Science of the total environment 2020 v.719 pp. 137498
ISSN:
0048-9697
Subject:
staining,   etc   ; acetone; automation; cameras; fluorescence; hydrogen peroxide; iron; microplastics;     Show all 8 Subjects
Abstract:
... Automated count of Nile Red fluorescent microplastics allows fast and reliable quantification. However, factors involving staining, digital camera conditions and settings introduce variability to the results. The objective of this paper is to identify and propose solutions to these factors and improve on the previous MP-VAT script. While removal of digital sensor defects had little influence on re ...
DOI:
10.1016/j.scitotenv.2020.137498

5. Application of hematoxylin reagent for sperm cell separation in sexual crime evidence

Author:
Kim, Joo-Young; Kim, Man Il; Lee, Hye Hyeon; Kim, Hye Lim; Lee, Eun-Jung; Lee, Yang-Han; Hwang, In-Kwan; Chun, Byung-Won; Kang, Pil-Won
Source:
Forensic science international 2020 v.307 pp. 110114
ISSN:
0379-0738
Subject:
staining,   etc   ; DNA; crime; forensic sciences; genotyping; microsatellite repeats; spermatozoa;     Show all 7 Subjects
Abstract:
... Seminal evidence obtained from a sexual crime scene provides clues for solving a case through forensic analysis. However, most evidence collected from sexual crime scenes is a mixture of sperm cells and vaginal discharge. Therefore, separating the sperm cells from the seminal evidence is very important. In this study, we developed a separation method for effectively separating sperm cells using di ...
DOI:
10.1016/j.forsciint.2019.110114

6. Three-way junction-induced isothermal amplification for nucleic acid detection

Author:
Lee, Seoyoung; Jang, Hyowon; Kim, Hyo Yong; Park, Hyun Gyu
Source:
Biosensors & bioelectronics 2020 v.147 pp. 111762
ISSN:
0956-5663
Subject:
staining,   etc   ; DNA; DNA-directed DNA polymerase; biosensors; fluorescence;     Show all 5 Subjects
Abstract:
... We, herein, describe a three-way junction (3WJ)-induced isothermal amplification (ThIsAmp) reaction for target nucleic acid detection. In this strategy, target nucleic acid induces the formation of 3WJ structure by associating a specially designed ThIsAmp template and ThIsAmp primer. Upon the formation of 3WJ structure, ThIsAmp primer is subjected to continuously repeated extension and nicking rea ...
DOI:
10.1016/j.bios.2019.111762

7. A stereological analysis on the calculation of the volume values of thoracic segments in ducks

Author:
Cakmak, Gamze; Cetin Ragbetli, Murat
Source:
Anatomia, histologia, embryologia 2020 v.49 no.1 pp. 17-24
ISSN:
0340-2096
Subject:
staining,   etc   ; Anas; adults; ducks; formaldehyde; gender; photographs; spinal cord; vertebrae;     Show all 9 Subjects
Abstract:
... Stereology is a method for examining two‐dimensional objects as three‐dimensional objects. The aim of this study was to offer volume values for thoracic segments in ducks by means of stereological methods. This study examined the data obtained from stereological analysis of the total volume and grey and white matter volume values of the thoracic segment, a part of the adult duck spinal cord with a ...
DOI:
10.1111/ahe.12435

8. The ABCA1 blocking agent probucol decreases capacitation in ejaculated dog spermatozoa

Author:
Schäfer-Somi, Sabine; Budik, Sven
Source:
Acta veterinaria scandinavica 2020 v.62 no.1 pp. 2
ISSN:
1751-0147
Subject:
staining,   etc   ; ABC transporters; acrosome; cell membranes; cholesterol; colorimetry; dogs; epididymis; phospholipids; probucol; ripening; sperm capacitation; viability;     Show all 13 Subjects
Abstract:
... BACKGROUND: The ATP binding cassette (ABC) transporters participate in the cholesterol and phospholipid transport within and through cell membranes of many cells including spermatozoa. Cholesterol efflux is important for capacitation of spermatozoa. ABCA1 expression has been assessed in canine spermatozoa previously but its role in capacitation still has to be determined. The aim of the study was ...
DOI:
10.1186/s13028-019-0500-2
PubMed:
31907058
PubMed Central:
PMC6945687

9. Visualization of internal 3D structure of small live seed on germination by laboratory-based X-ray microscopy with phase contrast computed tomography

Author:
Kunishima, Naoki; Takeda, Yoshihiro; Hirose, Raita; Kalasová, Dominika; Šalplachta, Jakub; Omote, Kazuhiko
Source:
Plant methods 2020 v.16 no.1 pp. 7
ISSN:
1746-4811
Subject:
staining,   etc   ; Viola; X-radiation; computed tomography; germination; image analysis; irradiation; microscopy; plant tissues; seeds;     Show all 10 Subjects
Abstract:
... BACKGROUND: The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) with a phase-retrieval technique. Recently, a laboratory-based X-ray microscope adopting the phase contrast CT was developed as a powerful tool for the observation of weakly absorb ...
DOI:
10.1186/s13007-020-0557-y
PubMed:
32021643
PubMed Central:
PMC6995115

10. Unbreakable and customizable dipping chambers for TLC and HPTLC manufactured by fused deposition modelling

Author:
Jaxel, Julien; Guggenberger, Matthias; Rosenau, Thomas; Böhmdorfer, Stefan
Source:
Talanta 2020 v.217 pp. 121072
ISSN:
0039-9140
Subject:
staining,   etc   ; cross contamination; derivatization; dipping; glass; jars; polypropylenes; thin layer chromatography;     Show all 8 Subjects
Abstract:
... Postchromatographic derivatization chambers for Thin Layer Chromatography (TLC) and its high-performance counterpart (HPTLC) are made of glass, which renders them fragile and – given their frequent use – eventually rather expensive. As a consequence, in daily lab routine, staining reagents are often kept in jars or beakers as cheap and available but unsatisfying equipment. This work describes the ...
DOI:
10.1016/j.talanta.2020.121072

11. Peroxidase zymograms obtained by agarose native gel electrophoresis have unmet resolution and completeness

Author:
Oloketuyi, Sandra; Annovi, Giulia; de Marco, Ario
Source:
International journal of biological macromolecules 2020 v.156 pp. 869-873
ISSN:
0141-8130
Subject:
staining,   etc   ; acrylamides; agarose; biomarkers; gel electrophoresis; gels; horseradish; peroxidase; protein isoforms;     Show all 9 Subjects
Abstract:
... A recently published method to separate protein isoforms by means of a flat-bed agarose native gel was adapted for identifying simultaneously both acid and basic isoforms of plant peroxidases. These were evidenced by in situ activity staining using alternative substrates for which the isoforms showed specific preference. Such approach allowed the detection of a significantly higher number of horse ...
DOI:
10.1016/j.ijbiomac.2020.04.058

12. PHF23 negatively regulates the autophagy of chondrocytes in osteoarthritis

Author:
Maimaitijuma, Talatibaike; Yu, Jia-Hong; Ren, Ya-Li; Yang, Xin; Liu, Heng; Meng, Zhi-Chao; Wang, Rui; Cui, Yun-Peng; Wu, Hao; Pan, Li-Ping; Jiao, Yang; Chen, Ying-Yu; Cao, Yong-Ping
Source:
Life sciences 2020 v.253 pp. 117750
ISSN:
0024-3205
Subject:
staining,   etc   ; Western blotting; autophagosomes; cell viability; chloroquine; chondrocytes; collagen; elderly; interleukins; lysosomes; mitophagy; osteoarthritis; phosphorylation; surgery;     Show all 14 Subjects
Abstract:
... Osteoarthritis (OA) is the main cause of disability and joint replacement surgery in the elderly. As a crucial cell survival mechanism, autophagy has been reported to decrease in OA. PHF23 is a new autophagy inhibitor which was first reported by us previously. This study aimed to explore the anti-autophagic mechanism of PHF23 to make it a possible therapeutic target of OA.Lentiviral vectors specif ...
DOI:
10.1016/j.lfs.2020.117750

13. Fluoroscopy-guided approach to the lumbar dorsal root ganglion in dogs: cadaver study in Beagles

Author:
Kim, Hyunseok; Son, Won-gyun; Shin, Chi Won; Han, Hyungjoo; Cha, Jeesoo; Kim, Dalhae; Lee, Inhyung
Source:
Veterinary anaesthesia and analgesia 2020 v.47 no.4 pp. 574-577
ISSN:
1467-2987
Subject:
staining,   etc   ; Beagle; cadaver; dogs; ganglia; methylene blue; nerve tissue; pain; standard deviation;     Show all 9 Subjects
Abstract:
... To evaluate an approach to the canine lumbar dorsal root ganglion (DRG), a significant contributor to the pain pathway, using new methylene blue staining.Prospective randomized study.A total of three Beagle dog cadavers weighing 10.4 ± 0.7 kg (mean ± standard deviation).Bilateral third to fifth lumbar DRG approaches were performed in three dog cadavers positioned in sternal recumbency. The mammill ...
DOI:
10.1016/j.vaa.2020.03.004

14. Open source software tool for the automated detection and characterization of epithelial cells from trace biological samples

Author:
Olsen, Anita; Miller, Mekhi; Yadavalli, Vamsi K.; Ehrhardt, Christopher J.
Source:
Forensic science international 2020 v.312 pp. 110300
ISSN:
0379-0738
Subject:
staining,   etc   ; automation; computer software; epithelial cells; fluorescent dyes; microscopes; nucleic acids; optical properties;     Show all 8 Subjects
Abstract:
... This paper presents a strategy for an unsupervised workflow for identifying epithelial cells in microscopic images and characterizing their morphological and/or optical properties. The proposed method can be used on cells that have been stained with fluorescent dyes and imaged using conventional optical microscopes. The workflow was tested on cell populations that were imaged directly on touch/con ...
DOI:
10.1016/j.forsciint.2020.110300

15. The Effect of Different Vitrification and Staining Protocols on the Visibility of the Nuclear Maturation Stage of Equine Oocytes

Author:
Pereira, Blasa; Ortiz, Isabel; Dorado, Jesus; Diaz-Jimenez, Maria; Consuegra, Cesar; Demyda-Peyras, Sebastian; Hidalgo, Manuel
Source:
Journal of equine veterinary science 2020 v.90 pp. 103021
ISSN:
0737-0806
Subject:
staining,   etc   ; carbon dioxide; chromatin; cryoprotectants; dimethyl sulfoxide; ethylene glycol; mares; metaphase; oocytes; propidium; sucrose; vitrification;     Show all 12 Subjects
Abstract:
... In this study, we compared two staining protocols assessing the nuclear chromatin stage of equine oocytes after vitrification using permeable and nonpermeable cryoprotectants. Slaughterhouse-derived oocytes (n = 155) were obtained from a total of 32 mares and in vitro matured in M199 medium for 42 hours at 38.5°C in 5% CO₂. In the first experiment, two concentrations of Hoechst 33342 (HO) were tes ...
DOI:
10.1016/j.jevs.2020.103021

16. Survey of characteristics of exposure to mainstream cigarette smoke using discarded cigarette butts from Korean smokers

Author:
Pack, Eun Chul; Kim, Hyung Soo; Lee, Seung Ha; Koo, Ye Ji; Jang, Dae Young; Choi, Seung Hyun; Kim, Ji Ae; Lim, Chae Hui; Lim, Kyung Min; Choi, Dal Woong
Source:
Environmental research 2020 v.185 pp. 109434
ISSN:
0013-9351
Subject:
staining,   etc   ; Koreans; cigarettes; humans; menthol; models; nicotine; physicochemical properties; prediction; regression analysis; smoke; smoking (habit); surveys;     Show all 13 Subjects
Abstract:
... Evaluating the characteristics of exposure to mainstream cigarette smoke is an essential field in tobacco research because of the large risk burden among smokers. Detailed evaluation of the complex factors pertaining to the exposure of smokers to mainstream cigarette smoke was attempted by analysis of discarded cigarette butts. A total of 5475 samples of discarded cigarette butts was collected to ...
DOI:
10.1016/j.envres.2020.109434

17. Vitrification of caprine secondary and early antral follicles as a perspective to preserve fertility function

Author:
Lopes, Everton Pimentel Ferreira; Rodrigues, Giovanna Quintino; de Brito, Danielle Cristina Calado; Rocha, Rebeca Magalhães Pedrosa; Ferreira, Anna Clara Accioly; de Sá, Naíza Arcângela Ribeiro; Silva, Renato Félix da; de Alcântara, Gabriel Las Heras; Alves, Benner Geraldo; Figueiredo, José Ricardo de; Zelinski, Mary; Rodrigues, Ana Paula Ribeiro
Source:
Reproductive Biology 2020
ISSN:
1642-431X
Subject:
staining,   etc   ; aromatase; cryopreservation; fluorescence; goats; in vitro culture; ovarian follicles; polymers; viability; vitrification;     Show all 10 Subjects
Abstract:
... The present study aimed to evaluate the structure, survival and development of isolated caprine (secondary-SEC and early antral-EANT) follicles, after vitrification in the presence of synthetic polymers and in vitro culture. Additionally, transzonal projections (TZPs) and p450 aromatase enzyme were evaluated. After isolation, SEC and EANT follicles were in vitro cultured for six days or vitrified. ...
DOI:
10.1016/j.repbio.2020.05.001

18. Effect of contrast and local anesthetic on dye spread following transversus abdominis plane injection in dog cadavers

Author:
de Miguel Garcia, Cristina; Whyte, Mackenzie; St James, Mariko; Ferreira, Tatiana H.
Source:
Veterinary anaesthesia and analgesia 2020 v.47 no.3 pp. 391-395
ISSN:
1467-2987
Subject:
staining,   etc   ; Beagle; anesthesia; cadaver; dogs; local anesthetics; methylene blue; nerve tissue; ultrasonics;     Show all 9 Subjects
Abstract:
... To determine whether the addition of bupivacaine or contrast medium to methylene blue dye would affect dye distribution following ultrasound (US)-guided transversus abdominis plane (TAP) injections.Prospective, randomized, blinded cadaveric study.A total of 29 fresh Beagle dog cadavers.Each hemiabdomen (n = 58) was randomized into one of three groups: group M, 1% methylene blue; group MB, 50:50 mi ...
DOI:
10.1016/j.vaa.2020.01.003

19. Quantitative magnetic resonance imaging and histological hoof wall assessment of 3‐year‐old Quarter Horses

Author:
Pownder, S. L.; Caserto, B. G.; Bowker, R. M.; Lin, B.; Potter, H. G.; Koff, M. F.
Source:
Equine veterinary journal 2020 v.52 no.3 pp. 435-440
ISSN:
0425-1644
Subject:
staining,   etc   ; Quarter Horse; collagen; feet; histology; hooves; horses; lameness; magnetic resonance imaging; proteoglycans; water content;     Show all 11 Subjects
Abstract:
... BACKGROUND: Few noninvasive methods are available for equine hoof wall evaluation. The highly organised wall structures and composition of proteoglycans and collagens may make this region amenable to quantitative MRI (qMRI) techniques of T1ρ and T2 mapping to identify pathology related to proteoglycan content and collagen organisation respectively. OBJECTIVE: To establish normative T1ρ and T2 valu ...
DOI:
10.1111/evj.13188

20. Characterization of Black Pigmenturia in a Cat with Apocrine Gland Cystadenocarcinoma

Author:
Fournier, Q.; Höhn, A.; Deubel, S.; Milne, E.; Bavcar, S.; Morrison, L.
Source:
Journal of comparative pathology 2020 v.176 pp. 145-150
ISSN:
0021-9975
Subject:
staining,   etc   ; advanced glycation end products; confocal microscopy; females; histopathology; light microscopy; metastasis; pentosidine; pigmentation; pigments; urine;     Show all 11 Subjects
Abstract:
... An 8-year-old neutered female domestic shorthair cat was presented for further management of an apocrine gland cystadenocarcinoma. Extensive nodal metastasis was diagnosed and the cat was humanely destroyed 2 months after presentation. Post-mortem histopathology of the cystadenocarcinoma revealed areas of yellow–brown granular pigmentation on light microscopy, staining positively for reducing subs ...
DOI:
10.1016/j.jcpa.2020.03.002