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staining, etc ; fluids; forensic sciences; medicine; spermatozoa; Show all 5 Subjects
Abstract:
... Much has been written about the presence or absence of spermatozoa in pre-ejaculate fluid (PEF). Published studies only have a small number of participants; their results are conflicting, may be inaccurate and findings have not reached statistical significance. There are potential methodical issues. However, upon review, the possibility exists that PEF may sometimes contain viable spermatozoa. Add ...
... In present study, a recyclable and environmentally friendly dyeing method through using extractives from Dalbergia cohinchinensis residues without any mordant as natural dye (ND) was studied with the objective of improving anti-UV property of the dyed wood. The dyeing mechanism was explored and the anti-UV property of ND dyed wood was evaluated. The results indicated that total color difference wa ...
staining, etc ; Orthoptera; abdominal ganglia; axons; interneurons; males; physiology; wings; Show all 8 Subjects
Abstract:
... Chirping male crickets combine a 30 Hz pulse pattern with a 3 Hz chirp pattern to drive the rhythmic opening-closing movements of the front wings for sound production. Lesion experiments suggest two coupled modular timer-networks located along the chain of abdominal ganglia, a network in A3 and A4 generating the pulse pattern, and a network organized along with ganglia A4–A6 controlling the genera ...
staining, etc ; automation; bioengineering; density; experts; humans; image analysis; journals; wills; Show all 9 Subjects
Abstract:
... Deep learning has emerged as a breakthrough tool for the segmentation of images without supporting human experts. Here, we propose an automated approach that uses deep learning to generate pseudo-nuclear staining of cells from phase contrast images. Our proposed approach, which has the feature to generate pseudo-nuclear stained images by simple DNN, showed remarkable advantages over existing appro ...
... Large amounts of water and leather chemicals such as sodium sulfide and calcium hydroxide are used in conventional leather production, resulting in huge amount of wastewater and serious environmental pollution. Enzyme is eco-benign, and an ideal alternative to conventional chemicals attributed to its excellent characteristics and high efficiency. In this work, enzymes with different ions are used ...
staining, etc ; acetates; acetylcholinesterase; albuminuria; bovine serum albumin; dyes; false positive results; gels; human serum albumin; humans; knowledge; neostigmine; purity; urine; Show all 14 Subjects
Abstract:
... Human serum albumin (HSA) has pseudoesterase activity. So far on gel specific detection of such property of HSA is never reported. Moreover, protein binding dyes are non-specific for albumin. However, many of such dyes are used for HSA detection. So, dye-based albumin detection on the gel is expected to generate false-positive results for HSA. In this context, we have discovered that Fast Blue BB ...
staining, etc ; acrylamides; agarose; biomarkers; gel electrophoresis; gels; horseradish; peroxidase; protein isoforms; Show all 9 Subjects
Abstract:
... A recently published method to separate protein isoforms by means of a flat-bed agarose native gel was adapted for identifying simultaneously both acid and basic isoforms of plant peroxidases. These were evidenced by in situ activity staining using alternative substrates for which the isoforms showed specific preference. Such approach allowed the detection of a significantly higher number of horse ...
staining, etc ; iron; polymers; ruthenium; scanning tunneling microscopy; spectroscopy; structure-activity relationships; Show all 7 Subjects
Abstract:
... During the past few decades, the study of the single polymer chain has attracted considerable attention with the goal of exploring the structure–property relationship of polymers. It still, however, remains challenging due to the variability and low atomic resolution of the amorphous single polymer chain. Here, we demonstrated a new strategy to visualize the single metallopolymer chain with a hexa ...
staining, etc ; analgesia; cadaver; dogs; methylene blue; nerve tissue; ultrasonics; ultrasonography; umbilicus; Show all 9 Subjects
Abstract:
... To describe the ultrasound-guided rectus sheath block technique and the anatomical spread of two volumes of methylene blue injection in dog cadavers.Blinded, prospective, experimental cadaveric study.A total of eight dog cadavers weighing 8.9 ± 1.6 kg.Ultrasound-guided rectus sheath injections were performed bilaterally 1 cm cranial to the umbilicus using 0.25 mL kg–¹ (low volume; LV) and 0.50 mL ...
Jocelyn H. Wright; Li-Ya Huang; Stephanie Weaver; L. Diego Archila; Megan S. McAfee; Alexandre V. Hirayama; Aude G. Chapuis; Marie Bleakley; Anthony Rongvaux; Cameron J. Turtle; R. Savanh Chanthaphavong; Jean S. Campbell; Robert H. Pierce
staining, etc ; Marmota monax; T-lymphocytes; antigens; biopsy; detection; flow cytometry; hepatitis; immunohistochemistry; immunophenotype; in situ hybridization; neoplasms; patients; permeability; phenotype; proteins; toxicity; Show all 17 Subjects
Abstract:
... Identifying engineered T cells in situ is important to understand the location, persistence, and phenotype of these cells in patients after adoptive T cell therapy. While engineered cells are routinely characterized in fresh tissue or blood from patients by flow cytometry, it is difficult to distinguish them from endogenous cells in formalin-fixed, paraffin-embedded (FFPE) tissue biopsies. To over ...
staining, etc ; biofilm; computer software; cutting; drugs; image analysis; ionic liquids; knowledge; osmium; pressure; protocols; research; ruthenium red; sampling; scanning electron microscopy; tannins; Show all 16 Subjects
Abstract:
... Several imaging methodologies have been used in biofilm studies, contributing to deepening the knowledge on their structure. This review illustrates the most widely used microscopy techniques in biofilm investigations, focusing on traditional and innovative scanning electron microscopy techniques such as scanning electron microscopy (SEM), variable pressure SEM (VP-SEM), environmental SEM (ESEM), ...
staining, etc ; evaporation; gravity; particles; surfaces; Show all 5 Subjects
Abstract:
... Evaporation of colloidal drops on horizontal surfaces deposits the contained particles at the drop-edge producing radially symmetric ring-like stains. The symmetry in the particle deposition is broken when the drop is placed on a tilted surface due to the influence of gravity on the suspended particles and the drop itself. Using extremely small drops generated by electrospray, we explore cases whe ...
staining, etc ; Beagle; anesthesia; cadaver; dogs; local anesthetics; methylene blue; nerve tissue; ultrasonics; Show all 9 Subjects
Abstract:
... To determine whether the addition of bupivacaine or contrast medium to methylene blue dye would affect dye distribution following ultrasound (US)-guided transversus abdominis plane (TAP) injections.Prospective, randomized, blinded cadaveric study.A total of 29 fresh Beagle dog cadavers.Each hemiabdomen (n = 58) was randomized into one of three groups: group M, 1% methylene blue; group MB, 50:50 mi ...
staining, etc ; anti-inflammatory activity; assays; breathing; curcumin; cytokines; drugs; engineering; eosin; epithelial cells; exosomes; in vivo studies; inflammation; lipopolysaccharides; lungs; macrophages; models; therapeutics; Show all 18 Subjects
Abstract:
... Exosomes have been investigated as delivery vesicles for various drugs. However, exosome-mediated peptide delivery into the lungs has not been studied. In this study, exosomes were engineered for the pulmonary delivery of RAGE-binding peptide (RBP), an anti-inflammatory peptide. To load the peptide into exosomes, RBP was linked to an exosome membrane integral protein, Lamp2b, to produce RBP-linked ...
staining, etc ; Megalobrama amblycephala; bone formation; economic valuation; fish; gene expression; genes; genome-wide association study; mineralization; osteoblasts; palatability; signal transduction; skeletal development; transcriptomics; Show all 14 Subjects
Abstract:
... Intermuscular bone (IB) is a hard-boned spicule exist in lower teleost, which brings a lot of detrimental effects on palatability and economic value of blunt snout bream (Megalobrama amblycephala). Masson trichrome staining for ossific IB indicated that some osteoblasts appeared at the edge of the bone matrix and a few osteocytes are present in the center of the mineralized bone matrix. By compari ...
... Polyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously described Sa. solfataricus polyP (-) strain and a new polyP (-) S. acidocaldarius strain generated in ...
Vincent H.J. van der Velden; Juan Flores-Montero; Martin Perez-Andres; Marta Martin-Ayuso; Oliver Crespo; Elena Blanco; Tomas Kalina; Jan Philippé; Carolien Bonroy; Maaike de Bie; Jeroen te Marvelde; Cristina Teodosio; Alba Corral Mateos; Veronika Kanderová; Mirjam van der Burg; Dennis Van Hoof; Jacques J.M. van Dongen; Alberto Orfao
staining, etc ; antibodies; fluorescence; immunosuppression; patients; screening; Show all 6 Subjects
Abstract:
... Within EuroFlow, we recently developed screening tubes for hematological malignancies and immune deficiencies. Pipetting of antibodies for such 8-color 12-marker tubes however is time-consuming and prone to operational mistakes. We therefore evaluated dried formats of the lymphocytosis screening tube (LST) and of the primary immune deficiency orientation tube (PIDOT). Both tubes were evaluated on ...
staining, etc ; Enterocytozoon; RNA; acridine orange; chitin; dyes; fluorescence; grapes; quantitative analysis; spores; sporophytes; Show all 11 Subjects
Abstract:
... A double fluorescent staining method was used to differentiate life stages of Enterocytozoon hepatopenaei (EHP) with a chitin-binding dye, calcofluor white (CFW) and a nucleic acid dye, acridine orange (AO). The unreleased spores showed a significantly blue fluorescent chitin wall with weak orange-red RNA staining, while the plasmodia appeared grape cluster-like orange-red nucleic acid-containing ...
staining, etc ; Cylindrotheca; chloroplasts; fluorescence; image analysis; rapid methods; refractive index; seawater; tomography; vacuoles; Show all 10 Subjects
Abstract:
... Optical diffraction tomography is an emerging label-free microscopic technique with its capability of label-free, quantitative, and rapid imaging of biological samples. In this work, we present the imaging and analysis of a living diatom Cylindrotheca sp. in seawater without using any pretreatment such as fluorescence staining. The 3D refractive index (RI) of a living diatom cell was measured, to ...
staining, etc ; automation; computer software; epithelial cells; fluorescent dyes; microscopes; nucleic acids; optical properties; Show all 8 Subjects
Abstract:
... This paper presents a strategy for an unsupervised workflow for identifying epithelial cells in microscopic images and characterizing their morphological and/or optical properties. The proposed method can be used on cells that have been stained with fluorescent dyes and imaged using conventional optical microscopes. The workflow was tested on cell populations that were imaged directly on touch/con ...