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1. A flexible and cost-effective manual droplet operation platform for miniaturized cell assays and single cell analysis

Author:
Lou, Qi; Ma, Yan; Zhao, Shi-Ping; Du, Guan-Sheng; Fang, Qun
Source:
Talanta 2021 v.224 pp. 121874
ISSN:
0039-9140
Subject:
staining,   etc   ; assays; automation; coculture; cost effectiveness; droplets; drugs; enzymes; laboratories; liquids; storage; syringes; washing;     Show all 13 Subjects
Abstract:
... Herein, we developed a flexible and cost-effective manual droplet operation system (MDOS) for performing miniaturized cell assays as well as single cell analysis. The MDOS consists of a manual x-y-z translation stage for liquid transferring and switching, a high-precision syringe pump for liquid driving and metering, a tapered capillary probe for droplet manipulation, a droplet array chip for drop ...
DOI:
10.1016/j.talanta.2020.121874

2. Optimization of immunohistochemical detection of rat ESR2 proteins with well-validated monoclonal antibody PPZ0506

Author:
Hattori, Yujiro; Ishii, Hirotaka; Higo, Shimpei; Otsuka, Mai; Kanaya, Moeko; Matsumoto, Keisuke; Ozawa, Mina; Ozawa, Hitoshi
Source:
Molecular and cellular endocrinology 2021 v.523 pp. 111145
ISSN:
0303-7207
Subject:
staining,   etc   ; antigens; cross reaction; detection; endocrinology; humans; immunohistochemistry; monoclonal antibodies; proteins; rats; research; researchers; tissues;     Show all 13 Subjects
Abstract:
... Although there are few well-validated antibodies against ESR2 proteins, a recent validation assessment identified a specific monoclonal antibody against human ESR2 proteins (PPZ0506). Furthermore, our previous study confirmed its cross-reactivity and specificity against rodent ESR2 proteins, enabling the determination of true ESR2 distribution profiles in rodents. Therefore, we aimed to determine ...
DOI:
10.1016/j.mce.2020.111145

3. Evaluation of the effect of ultrasound guidance on the accuracy of intercostal nerve injection: a canine cadaveric study

Author:
Thomson, Alexander C.S.; Portela, Diego A.; Romano, Marta; Otero, Pablo E.
Source:
Veterinary anaesthesia and analgesia 2021 v.48 no.2 pp. 256-263
ISSN:
1467-2987
Subject:
staining,   etc   ; accuracy; adults; analgesia; anesthesia; cadaver; dogs; dyes; injection; length; nerve tissue; pleura; ultrasonics; ultrasonography;     Show all 14 Subjects
Abstract:
... To develop an ultrasound-guided approach to intercostal nerve injection and to compare the success rate of intercostal nerve injections using blind or ultrasound-guided technique in canine cadavers.Prospective, randomized, descriptive, experimental anatomic study.A total of 14 mid-sized adult canine cadavers.Ultrasound landmarks were identified by dissection of four cadavers and used to develop an ...
DOI:
10.1016/j.vaa.2020.12.003

4. How to easily detect plant NADH-glutamate dehydrogenase (GDH) activity? A simple and reliable in planta procedure suitable for tissues, extracts and heterologous microbial systems

Author:
Marchi, L.; Degola, F.; Baruffini, E.; Restivo, F.M.
Source:
Plant science 2021 v.304 pp. 110714
ISSN:
0168-9452
Subject:
staining,   etc   ; environmental factors; extracts; genetics; glutamate dehydrogenase; isozymes; light; plant organs; tissues; variability;     Show all 10 Subjects
Abstract:
... Plant NADH glutamate dehydrogenase (GDH) is an intriguing enzyme, since it is involved in different metabolic processes owing to its reversible (anabolic/catabolic) activity and due to the oligomeric nature of the enzyme, that gives rise to several isoforms. The complexity of GDH isoenzymes pattern and the variability of the spatial and temporal localization of the different isoforms have limited ...
DOI:
10.1016/j.plantsci.2020.110714

5. Four new cycloartane triterpenoids from the leaves of Dysoxylum binectariferum

Author:
Yan, Hui-jiao; Si, Hong-li; Zhao, Hong-wei; Chen, Long; Yu, Jin-qian; Zhao, Heng-qiang; Wang, Xiao
Source:
Phytochemistry letters 2021 v.41 pp. 101-105
ISSN:
1874-3900
Subject:
staining,   etc   ; Dysoxylum gotadhora; apoptosis; assays; cells; cytotoxicity; leaves; plant biochemistry; spectral analysis; triterpenoids;     Show all 10 Subjects
Abstract:
... Four new cycloartane triterpenoids (1-4) were isolated from the leaves of Dysoxylum binectariferum. Their structures were elucidated on the basis of HRMS and NMR spectroscopic data. Compounds 1-3 represent a class of rare natural 24,25,26,27-tetranor type cycloartane triterpenoids. Compound 3 displayed moderate cytotoxicity against HCT-116 and DLD-1 with IC₅₀ values of 9.8 ± 0.3 μM and 12.0 ± 0.4 ...
DOI:
10.1016/j.phytol.2020.11.013

6. Antimicrobial Photoinactivation of In Situ Oral Biofilms by Visible Light Plus Water-Filtered Infrared A and Tetrahydroporphyrin-tetratosylate (THPTS)

Author:
Karygianni, Lamprini; Ruf, Sandra; Hellwig, Elmar; Follo, Marie; Vach, Kirstin; Al-Ahmad, Ali
Source:
Microorganisms 2021 v.9 no.1
ISSN:
2076-2607
Subject:
staining,   etc   ; adhesion; bacteria; biofilm; confocal laser scanning microscopy; irradiation; light; permeability; photosensitizing agents; sampling;     Show all 10 Subjects
Abstract:
... The aim of this study was to examine the effect of aPDT with visual light (VIS) + water-filtered infrared A (wIRA) as a light source, and tetrahydroporphyrin-tetratosylate (THPTS) as a photosensitizer on in situ initial and mature oral biofilms. The samples were incubated, ex situ, with THPTS for two minutes, followed by irradiation with 200 mW cm - 2 VIS + wIRA for five minutes at 37 °C. The adhe ...
DOI:
10.3390/microorganisms9010145

7. Comparison between two approaches for the transversus abdominis plane block in canine cadavers

Author:
Romano, Marta; Portela, Diego A.; Thomson, Alexander; Otero, Pablo E.
Source:
Veterinary anaesthesia and analgesia 2021 v.48 no.1 pp. 101-106
ISSN:
1467-2987
Subject:
staining,   etc   ; abdomen; analgesia; anesthesia; area; cadaver; dogs; ilium; injection; methylene blue; nerve tissue; ultrasonography; volume;     Show all 13 Subjects
Abstract:
... To compare the dye distribution following either two lateral abdominal or one lateral abdominal and one subcostal ultrasound-guided transversus abdominis plane (TAP) injections of a clinically relevant volume of dye solution in dogs.Randomized cadaveric study.A total of eight canine cadavers.On one side of each cadaver, two TAP injections were performed on the lateral aspect of the abdomen (approa ...
DOI:
10.1016/j.vaa.2020.09.005

8. Removal of excess chlorazol black stains from microcrustacean specimens

Author:
Mugnai, R.; Santos, A. S.; Corgosinho, P. H. C.; Salazar, S. S.
ISSN:
0011-216X
Subject:
staining,   etc   ; Biological Sciences; Crustacea; detergents; methanol; soaps;     Show all 6 Subjects
Abstract:
... The preparation of specimens is a crucial aspect of zoological studies. Some specific study techniques or certain taxonomic groups require staining processes. However, an excessive exposure to the staining agents can turn a specimen improper for morphological studies. Here, we report that an excess of chlorazol black, one of the most used stains in biology, can be easily removed from crustacean sp ...
DOI:
10.1163/15685403-bja10035

9. Analysis of microplastics in wetland samples from coastal Ghana using the Rose Bengal stain

Author:
Gbogbo, Francis; Takyi, James Benjamin; Billah, Maxwell Kelvin; Ewool, Julliet
Source:
Environmental monitoring and assessment 2020 v.192 no.4 pp. 208
ISSN:
0167-6369
Subject:
staining,   etc   ; color; microplastics; sediments; wetlands; Ghana;     Show all 6 Subjects
Abstract:
... The use of optical microscope remains the most commonly used technique for microplastic identification and quantification despite major limitations with misidentifications and biases. We evaluated the use of the 1% Rose Bengal stain in improving the identification of microplastics after a standard microplastic isolation process. The stain discriminated organic materials from potential microplastic ...
DOI:
10.1007/s10661-020-8175-8

10. Comparison of the structure of chinchilla sperm isolated from semen and from the tail of the epididymis

Author:
Andraszek, Katarzyna; Banaszewska, Dorota; Szeleszczuk, Olga; Niedbała, Piotr; Kuchta‐Gładysz, Marta
Source:
Reproduction in domestic animals 2020 v.55 no.4 pp. 515-522
ISSN:
0936-6768
Subject:
silver staining,   etc   ; acrosome; epididymis; semen; silver nitrate;     Show all 5 Subjects
Abstract:
... Sperm cells isolated from the tail of the epididymis and from the semen of the same individuals were analysed. The use of silver nitrate to stain sperm cells isolated from the tail of the epididymis made it possible to identify structures that were not visible in the sperm from semen. Silver nitrate very clearly distinguished the acrosomal and distal parts of the sperm head. Following silver nitra ...
DOI:
10.1111/rda.13646

11. The dead cell ratio of bacteria in sludge flocs as an indicator of sludge reduction in sludge ozone process

Author:
Hashimoto, Kurumi; Marushima, Toru; Nakai, Satoshi; Nishijima, Wataru; Motoshige, Hiroshi
Source:
Biochemical engineering journal 2020 v.154 pp. 107427
ISSN:
1369-703X
Subject:
staining,   etc   ; analytical methods; death; ozone; propidium; sludge;     Show all 6 Subjects
Abstract:
... A quantitative analytical method to determine the dead cell ratio of bacteria (death ratio) in sludge was developed for evaluating technologies and determining a sufficient amount of ozone for the sludge ozone process. The method with SYTO9 and propidium iodide as staining agents for live and dead cells, respectively, was optimized. The death ratio developed in this study could successfully predic ...
DOI:
10.1016/j.bej.2019.107427

12. Effect of different ions in assisting protease to open the collagen fiber bundles in leather making

Author:
Zhang, Yafei; Liu, Hui; Tang, Keyong; Liu, Jie; Li, Xiumin
Source:
Journal of cleaner production 2021 v.293 pp. 126017
ISSN:
0959-6526
Subject:
staining,   etc   ; calcium hydroxide; collagen; exhibitions; hydrogen; ions; leather; magnesium; mechanical properties; phosphates; pollution; proteinases; shrinkage; sodium sulfide; temperature; wastewater; water;     Show all 17 Subjects
Abstract:
... Large amounts of water and leather chemicals such as sodium sulfide and calcium hydroxide are used in conventional leather production, resulting in huge amount of wastewater and serious environmental pollution. Enzyme is eco-benign, and an ideal alternative to conventional chemicals attributed to its excellent characteristics and high efficiency. In this work, enzymes with different ions are used ...
DOI:
10.1016/j.jclepro.2021.126017

13. Pseudosterase activity-based specific detection of human serum albumin on gel

Author:
Kumar, Deepak; Bhattacharyya, Rajasri; Banerjee, Dibyajyoti
Source:
Talanta 2021 v.224 pp. 121906
ISSN:
0039-9140
Subject:
staining,   etc   ; acetates; acetylcholinesterase; albuminuria; bovine serum albumin; dyes; false positive results; gels; human serum albumin; humans; knowledge; neostigmine; purity; urine;     Show all 14 Subjects
Abstract:
... Human serum albumin (HSA) has pseudoesterase activity. So far on gel specific detection of such property of HSA is never reported. Moreover, protein binding dyes are non-specific for albumin. However, many of such dyes are used for HSA detection. So, dye-based albumin detection on the gel is expected to generate false-positive results for HSA. In this context, we have discovered that Fast Blue BB ...
DOI:
10.1016/j.talanta.2020.121906

14. Activation of MAPK signaling in response to nitrogen deficiency in Ulva prolifera (Chlorophyta)

Author:
Yang, Juanjuan; Yin, Yi; Yu, Dachun; He, Lihong; Shen, Songdong
Source:
Algal research 2021 v.53 pp. 102153
ISSN:
2211-9264
Subject:
staining,   etc   ; Ulva prolifera; Western blotting; algal blooms; apoptosis; assays; fluorescence; glutamine; macroalgae; mortality; nitrate reductase; nitrite reductase; nitrogen; protoplasts; research;     Show all 15 Subjects
Abstract:
... Ulva prolifera (Ulvophyceae, Chlorophyta), one of the main seaweeds species that cause “green tides”, suffers changes in nitrogen levels during drifting. However, the mechanisms of how this alga resist to nitrogen deficiency remains unclear. Transcripts encoding nitrate reductase, nitrite reductase, and glutamine synthase involved in nitrogen assimilation were amplified by RT-PCR, suggesting that ...
DOI:
10.1016/j.algal.2020.102153

15. Staining of wood veneers with anti-UV property using the natural dye extracted from Dalbergia cohinchinensis

Author:
Zhu, Tao; Sheng, Jiale; Chen, Jiabao; Ren, Kai; Wu, Zhenzeng; Wu, Hui; Li, Jian; Lin, Jinguo
Source:
Journal of cleaner production 2021 v.284 pp. 124770
ISSN:
0959-6526
Subject:
staining,   etc   ; Dalbergia; color; degradation; industry; lignin; natural dyes; reflectance; ultraviolet radiation; wavelengths; wood; wood fibers; wood veneer;     Show all 13 Subjects
Abstract:
... In present study, a recyclable and environmentally friendly dyeing method through using extractives from Dalbergia cohinchinensis residues without any mordant as natural dye (ND) was studied with the objective of improving anti-UV property of the dyed wood. The dyeing mechanism was explored and the anti-UV property of ND dyed wood was evaluated. The results indicated that total color difference wa ...
DOI:
10.1016/j.jclepro.2020.124770

16. Organic fluorescent probes for monitoring autophagy in living cells

Author:
Li, Xuechen; Liang, Xing; Yin, Junling; Lin, Weiying
Source:
Chemical Society reviews 2021 v.50 no.1 pp. 102-119
ISSN:
1460-4744
Subject:
staining,   etc   ; autophagy; bioactive properties; bioimaging; cells; degradation; design; detection; fluorescent dyes; monitoring; nervous system diseases; recycling; society;     Show all 13 Subjects
Abstract:
... As a ubiquitous degradation process in cells, autophagy plays important roles in various biological activities. However, the abnormality of autophagy is closely related to many diseases, such as aging, neurological disorder, and cancer. Thus, monitoring the process of autophagy in living cells has high significance in biological studies and diagnosis of related diseases. In order to real-time and ...
DOI:
10.1039/d0cs00896f

17. An ultrasound-guided subparaneural approach to the sciatic nerve in the dog: a cadaver study

Author:
Micieli, Fabiana; Chiavaccini, Ludovica; Mennonna, Giuseppina; Della Valle, Giovanni; Prisco, Francesco; Meomartino, Leonardo; Vesce, Giancarlo
Source:
Veterinary anaesthesia and analgesia 2021 v.48 no.1 pp. 107-115
ISSN:
1467-2987
Subject:
staining,   etc   ; Fossa; adults; analgesia; anesthesia; cadaver; catheters; dogs; injection; methylene blue; microscopy; nerve tissue; transducers (equipment); ultrasonics; ultrasonography;     Show all 15 Subjects
Abstract:
... To describe the gross and microscopic anatomy of the sciatic nerve paraneural sheath and to report an ultrasound (US)-guided subparaneural approach to the sciatic nerve in dogs, comparing two different volumes of injectate.Prospective, randomized, anatomical study.A group of nine middle-sized adult Mongrel canine cadavers (18 limbs).The sciatic nerves of three pelvic limbs of two canine cadavers w ...
DOI:
10.1016/j.vaa.2020.06.008

18. Crucial role of framework with cytoskeletal actin filaments for shaping microstructure of footpad setae in the ladybird beetle, Harmonia axyridis

Author:
Kimura, Ken-ichi; Hosoda, Naoe
Source:
Arthropod structure & development 2021 v.60 pp. 100998
ISSN:
1467-8039
Subject:
staining,   etc   ; Harmonia axyridis; actin; adhesion; arthropods; cells; cytochalasin D; hairs; insects; legs; microfilaments; microstructure; neurons; phalloidine; polymerization; pupae; surfaces;     Show all 17 Subjects
Abstract:
... Insects that can walk on smooth surfaces have specialized structures, footpads, on their legs. Footpads play an important role in adhesion to the substrate surface. Although the morphology and function of footpads have been studied, the mechanism of their formation is still elusive. In the ladybird beetle (Harmonia axyridis), hairy footpads are present on the first and second tarsal segments of th ...
DOI:
10.1016/j.asd.2020.100998

19. Cryopreservation of peripheral blood mononuclear cells for use in proliferation assays: First step towards potency assays

Author:
Juhl, Morten; Christensen, Jan Pravsgaard; Pedersen, Anders Elm; Kastrup, Jens; Ekblond, Annette
Source:
Journal of immunological methods 2021 v.488 pp. 112897
ISSN:
0022-1759
Subject:
staining,   etc   ; clinical trials; cryopreservation; cryoprotectants; dimethyl sulfoxide; fetal bovine serum; flow cytometry; immunosuppression; mononuclear leukocytes; phytohemagglutinin; product quality; therapeutics;     Show all 12 Subjects
Abstract:
... Investigational cell-based therapeutics are rapidly heading towards pivotal clinical trials. The premise is that the scientific rationale is well defined, and that product quality reflects exactly this. In vitro potency assays are necessary tools for evaluating cell products, and with potency assays comes high demands for standardization and reproducibility of the methods involved. For demonstrati ...
DOI:
10.1016/j.jim.2020.112897

20. Evaluation of injection volumes for the transversus abdominis plane block in dog cadavers: a preliminary trial

Author:
Freitag, Flavio A.V.; Muehlbauer, Eloisa; Gaio, Thalise daS.; dos Santos, Amanda A.M.; Machado, Marcello; Sanchez, Andrea; Duque, Juan C.M.
Source:
Veterinary anaesthesia and analgesia 2021 v.48 no.1 pp. 142-146
ISSN:
1467-2987
Subject:
staining,   etc   ; abdomen; adults; analgesia; anesthesia; cadaver; dogs; dyes; height; ilium; injection site; innervation; nerve tissue; ultrasonography; volume;     Show all 15 Subjects
Abstract:
... To evaluate staining of nerve branches after the injection of different volumes during ultrasound-guided transversus abdominis plane (TAP) block in dog cadavers.Prospective randomized study.A total of 15 frozen/thawed adult dog cadavers.Hemiabdomens were randomly allocated to one of four groups. In groups G0.3, G0.6 and G1.0, ropivacaine–methylene blue solution (0.3, 0.6 and 1.0 mL kg–¹ in seven, ...
DOI:
10.1016/j.vaa.2020.10.001