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1. Pre-ejaculate fluid in the context of sexual assault: A review of the literature from a clinical forensic medicine perspective

Author:
Maire C. Kelly
Source:
Forensic science international 2021 v.318 pp. 110596
ISSN:
0379-0738
Subject:
staining,   etc   ; fluids; forensic sciences; medicine; spermatozoa;     Show all 5 Subjects
Abstract:
... Much has been written about the presence or absence of spermatozoa in pre-ejaculate fluid (PEF). Published studies only have a small number of participants; their results are conflicting, may be inaccurate and findings have not reached statistical significance. There are potential methodical issues. However, upon review, the possibility exists that PEF may sometimes contain viable spermatozoa. Add ...
DOI:
10.1016/j.forsciint.2020.110596

2. Staining of wood veneers with anti-UV property using the natural dye extracted from Dalbergia cohinchinensis

Author:
Tao Zhu; Jiale Sheng; Jiabao Chen; Kai Ren; Zhenzeng Wu; Hui Wu; Jian Li; Jinguo Lin
Source:
Journal of cleaner production 2021 v.284 pp. 124770
ISSN:
0959-6526
Subject:
staining,   etc   ; Dalbergia; color; degradation; industry; lignin; natural dyes; reflectance; ultraviolet radiation; wavelengths; wood; wood fibers; wood veneer;     Show all 13 Subjects
Abstract:
... In present study, a recyclable and environmentally friendly dyeing method through using extractives from Dalbergia cohinchinensis residues without any mordant as natural dye (ND) was studied with the objective of improving anti-UV property of the dyed wood. The dyeing mechanism was explored and the anti-UV property of ND dyed wood was evaluated. The results indicated that total color difference wa ...
DOI:
10.1016/j.jclepro.2020.124770

3. Modular timer networks: abdominal interneurons controlling the chirp and pulse pattern in a cricket calling song

Author:
Pedro F. Jacob; Berthold Hedwig
Source:
Journal of comparative physiology 2020 v.206 no.6 pp. 921-938
ISSN:
0340-7594
Subject:
staining,   etc   ; Orthoptera; abdominal ganglia; axons; interneurons; males; physiology; wings;     Show all 8 Subjects
Abstract:
... Chirping male crickets combine a 30 Hz pulse pattern with a 3 Hz chirp pattern to drive the rhythmic opening-closing movements of the front wings for sound production. Lesion experiments suggest two coupled modular timer-networks located along the chain of abdominal ganglia, a network in A3 and A4 generating the pulse pattern, and a network organized along with ganglia A4–A6 controlling the genera ...
DOI:
10.1007/s00359-020-01448-0

4. Pseudo-nuclear staining of cells by deep learning improves the accuracy of automated cell counting in a label-free cellular population

Author:
Yuji Tsuzuki; Sho Sanami; Kenji Sugimoto; Satoshi Fujita
Source:
Journal of bioscience and bioengineering 2021 v.131 no.2 pp. 213-218
ISSN:
1389-1723
Subject:
staining,   etc   ; automation; bioengineering; density; experts; humans; image analysis; journals; wills;     Show all 9 Subjects
Abstract:
... Deep learning has emerged as a breakthrough tool for the segmentation of images without supporting human experts. Here, we propose an automated approach that uses deep learning to generate pseudo-nuclear staining of cells from phase contrast images. Our proposed approach, which has the feature to generate pseudo-nuclear stained images by simple DNN, showed remarkable advantages over existing appro ...
DOI:
10.1016/j.jbiosc.2020.09.014

5. Effect of different ions in assisting protease to open the collagen fiber bundles in leather making

Author:
Yafei Zhang; Hui Liu; Keyong Tang; Jie Liu; Xiumin Li
Source:
Journal of cleaner production 2021 v.293 pp. 126017
ISSN:
0959-6526
Subject:
staining,   etc   ; calcium hydroxide; collagen; exhibitions; hydrogen; ions; leather; magnesium; mechanical properties; phosphates; pollution; proteinases; shrinkage; sodium sulfide; temperature; wastewater; water;     Show all 17 Subjects
Abstract:
... Large amounts of water and leather chemicals such as sodium sulfide and calcium hydroxide are used in conventional leather production, resulting in huge amount of wastewater and serious environmental pollution. Enzyme is eco-benign, and an ideal alternative to conventional chemicals attributed to its excellent characteristics and high efficiency. In this work, enzymes with different ions are used ...
DOI:
10.1016/j.jclepro.2021.126017

6. Pseudosterase activity-based specific detection of human serum albumin on gel

Author:
Deepak Kumar; Rajasri Bhattacharyya; Dibyajyoti Banerjee
Source:
Talanta 2021 v.224 pp. 121906
ISSN:
0039-9140
Subject:
staining,   etc   ; acetates; acetylcholinesterase; albuminuria; bovine serum albumin; dyes; false positive results; gels; human serum albumin; humans; knowledge; neostigmine; purity; urine;     Show all 14 Subjects
Abstract:
... Human serum albumin (HSA) has pseudoesterase activity. So far on gel specific detection of such property of HSA is never reported. Moreover, protein binding dyes are non-specific for albumin. However, many of such dyes are used for HSA detection. So, dye-based albumin detection on the gel is expected to generate false-positive results for HSA. In this context, we have discovered that Fast Blue BB ...
DOI:
10.1016/j.talanta.2020.121906

7. Peroxidase zymograms obtained by agarose native gel electrophoresis have unmet resolution and completeness

Author:
Sandra Oloketuyi; Giulia Annovi; Ario de Marco
Source:
International journal of biological macromolecules 2020 v.156 pp. 869-873
ISSN:
0141-8130
Subject:
staining,   etc   ; acrylamides; agarose; biomarkers; gel electrophoresis; gels; horseradish; peroxidase; protein isoforms;     Show all 9 Subjects
Abstract:
... A recently published method to separate protein isoforms by means of a flat-bed agarose native gel was adapted for identifying simultaneously both acid and basic isoforms of plant peroxidases. These were evidenced by in situ activity staining using alternative substrates for which the isoforms showed specific preference. Such approach allowed the detection of a significantly higher number of horse ...
DOI:
10.1016/j.ijbiomac.2020.04.058

8. Synthesis of Metallopolymers and Direct Visualization of the Single Polymer Chain

Author:
Zhikai Li; Yiming Li; Yiming Zhao; Heng Wang; Yuan Zhang; Bo Song; Xiaohong Li; Shuai Lu; Xin-Qi Hao; Saw-Wai Hla; Yingfeng Tu; Xiaopeng Li
Source:
Journal of the American Chemical Society 2020 v.142 no.13 pp. 6196-6205
ISSN:
1520-5126
Subject:
staining,   etc   ; iron; polymers; ruthenium; scanning tunneling microscopy; spectroscopy; structure-activity relationships;     Show all 7 Subjects
Abstract:
... During the past few decades, the study of the single polymer chain has attracted considerable attention with the goal of exploring the structure–property relationship of polymers. It still, however, remains challenging due to the variability and low atomic resolution of the amorphous single polymer chain. Here, we demonstrated a new strategy to visualize the single metallopolymer chain with a hexa ...
DOI:
10.1021/jacs.0c00110

9. Ultrasound-guided rectus sheath block: an anatomic study in dog cadavers

Author:
Mariko St James; Tatiana H. Ferreira; Carrie A. Schroeder; Karen L. Hershberger-Braker; Kristopher M. Schroeder
Source:
Veterinary anaesthesia and analgesia 2020 v.47 no.1 pp. 95-102
ISSN:
1467-2987
Subject:
staining,   etc   ; analgesia; cadaver; dogs; methylene blue; nerve tissue; ultrasonics; ultrasonography; umbilicus;     Show all 9 Subjects
Abstract:
... To describe the ultrasound-guided rectus sheath block technique and the anatomical spread of two volumes of methylene blue injection in dog cadavers.Blinded, prospective, experimental cadaveric study.A total of eight dog cadavers weighing 8.9 ± 1.6 kg.Ultrasound-guided rectus sheath injections were performed bilaterally 1 cm cranial to the umbilicus using 0.25 mL kg–¹ (low volume; LV) and 0.50 mL ...
DOI:
10.1016/j.vaa.2019.09.001

10. Detection of engineered T cells in FFPE tissue by multiplex in situ hybridization and immunohistochemistry

Author:
Jocelyn H. Wright; Li-Ya Huang; Stephanie Weaver; L. Diego Archila; Megan S. McAfee; Alexandre V. Hirayama; Aude G. Chapuis; Marie Bleakley; Anthony Rongvaux; Cameron J. Turtle; R. Savanh Chanthaphavong; Jean S. Campbell; Robert H. Pierce
Source:
Journal of immunological methods 2021 v.492 pp. 112955
ISSN:
0022-1759
Subject:
staining,   etc   ; Marmota monax; T-lymphocytes; antigens; biopsy; detection; flow cytometry; hepatitis; immunohistochemistry; immunophenotype; in situ hybridization; neoplasms; patients; permeability; phenotype; proteins; toxicity;     Show all 17 Subjects
Abstract:
... Identifying engineered T cells in situ is important to understand the location, persistence, and phenotype of these cells in patients after adoptive T cell therapy. While engineered cells are routinely characterized in fresh tissue or blood from patients by flow cytometry, it is difficult to distinguish them from endogenous cells in formalin-fixed, paraffin-embedded (FFPE) tissue biopsies. To over ...
DOI:
10.1016/j.jim.2020.112955

11. Microscopy Methods for Biofilm Imaging: Focus on SEM and VP-SEM Pros and Cons

Author:
Michela Relucenti; Giuseppe Familiari; Orlando Donfrancesco; Maurizio Taurino; Xiaobo Li; Rui Chen; Marco Artini; Rosanna Papa; Laura Selan
Source:
Biology 2021 v.10 no.1 pp. -
ISSN:
2079-7737
Subject:
staining,   etc   ; biofilm; computer software; cutting; drugs; image analysis; ionic liquids; knowledge; osmium; pressure; protocols; research; ruthenium red; sampling; scanning electron microscopy; tannins;     Show all 16 Subjects
Abstract:
... Several imaging methodologies have been used in biofilm studies, contributing to deepening the knowledge on their structure. This review illustrates the most widely used microscopy techniques in biofilm investigations, focusing on traditional and innovative scanning electron microscopy techniques such as scanning electron microscopy (SEM), variable pressure SEM (VP-SEM), environmental SEM (ESEM), ...
DOI:
10.3390/biology10010051

12. Evaporation of Inclined Drops: Formation of Asymmetric Ring Patterns

Author:
Maheshwar Gopu; Sachin Rathod; Uma Namangalam; Ravi Kumar Pujala; S. Sunil Kumar; Dileep Mampallil
Source:
Langmuir 2020 v.36 no.28 pp. 8137-8143
ISSN:
1520-5827
Subject:
staining,   etc   ; evaporation; gravity; particles; surfaces;     Show all 5 Subjects
Abstract:
... Evaporation of colloidal drops on horizontal surfaces deposits the contained particles at the drop-edge producing radially symmetric ring-like stains. The symmetry in the particle deposition is broken when the drop is placed on a tilted surface due to the influence of gravity on the suspended particles and the drop itself. Using extremely small drops generated by electrospray, we explore cases whe ...
DOI:
10.1021/acs.langmuir.0c01084

13. Effect of contrast and local anesthetic on dye spread following transversus abdominis plane injection in dog cadavers

Author:
Cristina de Miguel Garcia; Mackenzie Whyte; Mariko St James; Tatiana H. Ferreira
Source:
Veterinary anaesthesia and analgesia 2020 v.47 no.3 pp. 391-395
ISSN:
1467-2987
Subject:
staining,   etc   ; Beagle; anesthesia; cadaver; dogs; local anesthetics; methylene blue; nerve tissue; ultrasonics;     Show all 9 Subjects
Abstract:
... To determine whether the addition of bupivacaine or contrast medium to methylene blue dye would affect dye distribution following ultrasound (US)-guided transversus abdominis plane (TAP) injections.Prospective, randomized, blinded cadaveric study.A total of 29 fresh Beagle dog cadavers.Each hemiabdomen (n = 58) was randomized into one of three groups: group M, 1% methylene blue; group MB, 50:50 mi ...
DOI:
10.1016/j.vaa.2020.01.003

14. Engineering exosomes for pulmonary delivery of peptides and drugs to inflammatory lung cells by inhalation

Author:
GyeungYun Kim; Youngki Lee; Junkyu Ha; Sangrok Han; Minhyung Lee
Source:
Journal of controlled release 2021 v.330 pp. 684-695
ISSN:
0168-3659
Subject:
staining,   etc   ; anti-inflammatory activity; assays; breathing; curcumin; cytokines; drugs; engineering; eosin; epithelial cells; exosomes; in vivo studies; inflammation; lipopolysaccharides; lungs; macrophages; models; therapeutics;     Show all 18 Subjects
Abstract:
... Exosomes have been investigated as delivery vesicles for various drugs. However, exosome-mediated peptide delivery into the lungs has not been studied. In this study, exosomes were engineered for the pulmonary delivery of RAGE-binding peptide (RBP), an anti-inflammatory peptide. To load the peptide into exosomes, RBP was linked to an exosome membrane integral protein, Lamp2b, to produce RBP-linked ...
DOI:
10.1016/j.jconrel.2020.12.053

15. Genome-wide analysis of intermuscular bone development reveals changes of key genes expression and signaling pathways in blunt snout bream (Megalobrama amblycephala)

Author:
Jing Chen; Xiuli Chen; Xin Huang; Guanghua Huang; Zexia Gao; Weimin Wang; Han Liu
Source:
Genomics 2021 v.113 no.1 pp. 654-663
ISSN:
0888-7543
Subject:
staining,   etc   ; Megalobrama amblycephala; bone formation; economic valuation; fish; gene expression; genes; genome-wide association study; mineralization; osteoblasts; palatability; signal transduction; skeletal development; transcriptomics;     Show all 14 Subjects
Abstract:
... Intermuscular bone (IB) is a hard-boned spicule exist in lower teleost, which brings a lot of detrimental effects on palatability and economic value of blunt snout bream (Megalobrama amblycephala). Masson trichrome staining for ossific IB indicated that some osteoblasts appeared at the edge of the bone matrix and a few osteocytes are present in the center of the mineralized bone matrix. By compari ...
DOI:
10.1016/j.ygeno.2020.09.062

16. The Role of Polyphosphate in Motility, Adhesion, and Biofilm Formation in Sulfolobales

Author:
Alejandra Recalde; Marleen van Wolferen; Shamphavi Sivabalasarma; Sonja-Verena Albers; Claudio A. Navarro; Carlos A. Jerez
Source:
Microorganisms 2021 v.9 no.1 pp. -
ISSN:
2076-2607
Subject:
staining,   etc   ; 4',6-diamidino-2-phenylindole; Sulfolobales; adhesion; biofilm; cells; energy; exopolyphosphatase; fluorescence microscopy; genes; glass; microorganisms; orthophosphates; phosphorylation; polymers; polyphosphates; strains; surfaces;     Show all 18 Subjects
Abstract:
... Polyphosphates (polyP) are polymers of orthophosphate residues linked by high-energy phosphoanhydride bonds that are important in all domains of life and function in many different processes, including biofilm development. To study the effect of polyP in archaeal biofilm formation, our previously described Sa. solfataricus polyP (-) strain and a new polyP (-) S. acidocaldarius strain generated in ...
DOI:
10.3390/microorganisms9010193

17. Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples

Author:
Vincent H.J. van der Velden; Juan Flores-Montero; Martin Perez-Andres; Marta Martin-Ayuso; Oliver Crespo; Elena Blanco; Tomas Kalina; Jan Philippé; Carolien Bonroy; Maaike de Bie; Jeroen te Marvelde; Cristina Teodosio; Alba Corral Mateos; Veronika Kanderová; Mirjam van der Burg; Dennis Van Hoof; Jacques J.M. van Dongen; Alberto Orfao
Source:
Journal of immunological methods 2019 v.475 pp. 112287
ISSN:
0022-1759
Subject:
staining,   etc   ; antibodies; fluorescence; immunosuppression; patients; screening;     Show all 6 Subjects
Abstract:
... Within EuroFlow, we recently developed screening tubes for hematological malignancies and immune deficiencies. Pipetting of antibodies for such 8-color 12-marker tubes however is time-consuming and prone to operational mistakes. We therefore evaluated dried formats of the lymphocytosis screening tube (LST) and of the primary immune deficiency orientation tube (PIDOT). Both tubes were evaluated on ...
DOI:
10.1016/j.jim.2017.03.011

18. A double staining method using calcofluor white and acridine orange to differentiate life stages of Enterocytozoon hepatopenaei (EHP) on hepatopancreatic sections

Author:
Wen Gao; Xiao-Meng Guo; Hai-Liang Wang; Ruo-Heng Zhao; Guo-Si Xie; Chen Li; Jie Huang
Source:
Aquaculture 2020 v.528 pp. 735628
ISSN:
0044-8486
Subject:
staining,   etc   ; Enterocytozoon; RNA; acridine orange; chitin; dyes; fluorescence; grapes; quantitative analysis; spores; sporophytes;     Show all 11 Subjects
Abstract:
... A double fluorescent staining method was used to differentiate life stages of Enterocytozoon hepatopenaei (EHP) with a chitin-binding dye, calcofluor white (CFW) and a nucleic acid dye, acridine orange (AO). The unreleased spores showed a significantly blue fluorescent chitin wall with weak orange-red RNA staining, while the plasmodia appeared grape cluster-like orange-red nucleic acid-containing ...
DOI:
10.1016/j.aquaculture.2020.735628

19. Label-free imaging and analysis of subcellular parts of a living diatom cylindrotheca sp. using optical diffraction tomography

Author:
Kazuo Umemura; Yuji Matsukawa; Yuki Ide; Shigeki Mayama
Source:
MethodsX 2020 v.7 pp. 100889
ISSN:
2215-0161
Subject:
staining,   etc   ; Cylindrotheca; chloroplasts; fluorescence; image analysis; rapid methods; refractive index; seawater; tomography; vacuoles;     Show all 10 Subjects
Abstract:
... Optical diffraction tomography is an emerging label-free microscopic technique with its capability of label-free, quantitative, and rapid imaging of biological samples. In this work, we present the imaging and analysis of a living diatom Cylindrotheca sp. in seawater without using any pretreatment such as fluorescence staining. The 3D refractive index (RI) of a living diatom cell was measured, to ...
DOI:
10.1016/j.mex.2020.100889
PubMed:
32382522
PubMed Central:
PMC7199005

20. Open source software tool for the automated detection and characterization of epithelial cells from trace biological samples

Author:
Anita Olsen; Mekhi Miller; Vamsi K. Yadavalli; Christopher J. Ehrhardt
Source:
Forensic science international 2020 v.312 pp. 110300
ISSN:
0379-0738
Subject:
staining,   etc   ; automation; computer software; epithelial cells; fluorescent dyes; microscopes; nucleic acids; optical properties;     Show all 8 Subjects
Abstract:
... This paper presents a strategy for an unsupervised workflow for identifying epithelial cells in microscopic images and characterizing their morphological and/or optical properties. The proposed method can be used on cells that have been stained with fluorescent dyes and imaged using conventional optical microscopes. The workflow was tested on cell populations that were imaged directly on touch/con ...
DOI:
10.1016/j.forsciint.2020.110300