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1. Generating a fucose permease deletion mutant in Bifidobacterium longum subspecies infantis ATCC 15697

Author:
Higgins, Melanie A.; Ryan, Katherine S.
Source:
Anaerobe 2021 pp. 102320
ISSN:
1075-9964
Subject:
strains,   etc   ; Bifidobacterium longum subsp. infantis; DNA; carbon; fucose; models; mutants;     Show all 7 Subjects
Abstract:
... Bifidobacterium longum subsp. infantis ATCC 15697 has emerged as a model for infant gut-associated bifidobacterial strains. Here we present a genetic system for B. longum subsp. infantis ATCC 15697 using its own DNA restriction-modification systems and create a fucose permease deletion mutant lacking the ability to use free fucose as a carbon source. ...
DOI:
10.1016/j.anaerobe.2021.102320

2. Low prevalence of Cutibacterium acnes in prostatic tissue biopsies in a French hospital

Author:
Bidaud, A.-L.; Karam, G.; Kandel-Aznar, C.; Ruffier d’Epenoux, L.; Guillouzouic, A.; Bémer, P.; Leroy, A.-G.; Corvec, S.
Source:
Anaerobe 2020 v.66 pp. 102286
ISSN:
1075-9964
Subject:
strains,   etc   ; biopsy; hospitals; phylotype; prevalence;     Show all 5 Subjects
Abstract:
... We evaluated the Cutibacterium acnes prevalence in prostatic biopsies and characterized the strains at a molecular level. 18 out of 36 biopsies (50%) were sterile after seven days in culture. C. acnes was observed in only two biopsies. Its prevalence was low (5.6%). Finally, the molecular characterization revealed diverse clusters including phylotypes IA₁, IB and II. ...
DOI:
10.1016/j.anaerobe.2020.102286

3. Carriage of Clostridioides difficile in healthy infants in the community of Handan, China: A 1-year follow-up study

Author:
Cui, Qing-qing; Yang, Jing; Sun, Su-ju; Li, Zhi-rong; Qiang, Cui-xin; Niu, Ya-nan; Li, Ru-xin; Shi, Dong-yan; Wei, Hong-lian; Tian, Tian-tian; Xu, Kai-yue; Wang, Wei-gang; Zhao, Jian-hong
Source:
Anaerobe 2021 v.67 pp. 102295
ISSN:
1075-9964
Subject:
nontoxigenic strains; toxigenic strains,   etc   ; Chinese people; Clostridium difficile; bacterial colonization; boys; carrier state; children; feces; feeding patterns; girls; infants; intestinal microorganisms; polymerase chain reaction; ribotypes; ribotyping; China;     Show all 17 Subjects
Abstract:
... Clostridioides difficile may colonize healthy infants and young children asymptomatically and for the long-term. C. difficile genotypes and the rate and determinants of colonization differ substantially and vary among countries and regions. A 1-year follow-up study was performed to determine the incidence, kinetics and influencing factors of C. difficile intestinal colonization.Twenty-nine healthy ...
DOI:
10.1016/j.anaerobe.2020.102295

4. Dogs are carriers of Clostridioides difficile lineages associated with human community-acquired infections

Author:
Bjöersdorff, Olivia Graaf; Lindberg, Sanna; Kiil, Kristoffer; Persson, Søren; Guardabassi, Luca; Damborg, Peter
Source:
Anaerobe 2021 v.67 pp. 102317
ISSN:
1075-9964
Subject:
nontoxigenic strains; virulent strains,   etc   ; Clostridium difficile; antibiotic resistance; clindamycin; databases; dogs; enrichment culture; erythromycin; genes; genetic variation; humans; metronidazole; nucleotide sequences; prevalence; public gardens; rifampicin; sampling; vancomycin; zoonoses;     Show all 20 Subjects
Abstract:
... There is an increasing concern about the role of animals as reservoirs of Clostridioides difficile. In this study, we investigated prevalence, antimicrobial resistance and zoonotic potential of C. difficile in dogs. Two-hundred and twenty-five dog faecal deposits were collected from trashcans in nine public gardens. C. difficile was isolated using selective plating and enrichment culture, identifi ...
DOI:
10.1016/j.anaerobe.2020.102317

5. Assembly of hyperhalophilic complex consortia of isolates from anchovy ripening attaining histamine degradation and their microbiome configuration

Author:
Perez, Silvina; Corti-Monzón, Georgina; Yeannes, María I.; Zaritzky, Noemí E.; Villegas-Plazas, Marcela; Junca, Howard; Murialdo, Silvia E.
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2021 v.142 pp. 111010
ISSN:
0023-6438
Subject:
multiple strain starters,   etc   ; Haloarcula; Halomonas; anchovies; culture media; food safety; foods; genes; histamine; microbiome; prokaryotic cells;     Show all 11 Subjects
Abstract:
... High histamine content in salted-ripened fish results in food safety detriment. The present study focused on the assembly of mixed cultures from halophilic prokaryotes isolated during salting-ripening anchovy process in order to determine their histamine-degrading capacity. For this purpose, 57 halophilic prokaryotes were inoculated together and submitted to several enrichment steps with strong sh ...
DOI:
10.1016/j.lwt.2021.111010

6. Do entomopathogenic nematodes induce immune priming?

Author:
Texca Tatevari, Méndez-López; Jorge, Contreras-Garduño; Luis, Mendoza-Cuenca; Ricardo, Ramirez-Romero
Source:
Microbial pathogenesis 2021 v.154 pp. 104844
ISSN:
0882-4010
Subject:
virulent strains,   etc   ; ; Nematoda; Tenebrio molitor; carbon dioxide; entomopathogenic nematodes; immune evasion; metabolism; pathogenesis; virulence;     Show all 10 Subjects
Abstract:
... Although the study of immune priming in insects is a growing area of research, its occurrence in various biological models has not been evaluated, and its mechanisms are poorly understood. Whether entomopathogenic nematodes (EPNs) can induce immune priming and what role their virulence might play in it has not been assessed. Here, we tested for the first time: 1) whether a nematode is capable of e ...
DOI:
10.1016/j.micpath.2021.104844

7. Identification of isocyclosporins by collision-induced dissociation of doubly protonated species

Author:
Baranova, Anna A.; Chistov, Alexey A.; Shuvalov, Maxim V.; Tyurin, Anton P.; Biryukov, Mikhail V.; Ivanov, Igor A.; Sadykova, Vera S.; Kurakov, Aleksander V.; Sergeeva, Albina I.; Korshun, Vladimir A.; Alferova, Vera A.
Source:
Talanta 2021 v.225 pp. 121930
ISSN:
0039-9140
Subject:
strains,   etc   ; Tolypocladium inflatum; acids; autoimmune diseases; cyclosporine; dissociation; fungi; immunosuppression; ions; isomerization; therapeutics; valine;     Show all 12 Subjects
Abstract:
... Nonribosomal cyclopeptide cyclosporin A (CsA), produced by fungus Tolypocladium inflatum, is an extremely important immunosuppressive drug used in organ transplantations and for therapy of autoimmune diseases. Here we report for the first time production of CsA, along with related cyclosporins B and C, by Tolypocladium inflatum strains of marine origin (White Sea). Cyclosporins A–C contain an unus ...
DOI:
10.1016/j.talanta.2020.121930

8. Metacytofilin has potent anti-malarial activity

Author:
Leesombun, Arpron; Iijima, Masatomi; Pagmadulam, Baldorj; Orkhon, Banzragchgarav; Doi, Hiroyasu; Issiki, Kunio; Sawa, Ryuichi; Nihei, Coh-ichi; Nishikawa, Yoshifumi
Source:
Parasitology international 2021 v.81 pp. 102267
ISSN:
1383-5769
Subject:
strains,   etc   ; Metarhizium; Plasmodium falciparum; artemisinin; chloroquine; death; drug resistance; erythrocytes; fungi; lead; optical isomers; parasites; parasitology;     Show all 13 Subjects
Abstract:
... Metacytofilin (MCF) was isolated from the fungus Metarhizium sp. TA2759. Although MCF possesses anti-Toxoplasma activity, the effects of this compound against other parasites are unknown. Here, we evaluated the in vitro anti-malarial activity of MCF against the 3D7 strain and the chloroquine-resistant K1 strain of Plasmodium falciparum. The half maximal inhibitory concentrations (IC₅₀) of MCF agai ...
DOI:
10.1016/j.parint.2020.102267

9. Sesquiterpene derivatives from Ocotea minarum leaves

Author:
Nogueira, Cláudio Rodrigo; Carbonezi, Lidilhone Hamerski; de Oliveira, Caroline Tieppo Flores; Garcez, Walmir Silva; Garcez, Fernanda Rodrigues
Source:
Phytochemistry letters 2021 v.42 pp. 8-14
ISSN:
1874-3900
Subject:
strains,   etc   ; Candida; Cryptococcus neoformans; Ocotea; chemical structure; leaves; minimum inhibitory concentration; plant biochemistry; sesquiterpenoids;     Show all 9 Subjects
Abstract:
... (+)-β-Sesquiphellandrene-12-oic acid (1) and 4-oxo-lanceolic acid (3), two new bisabolane derivatives; (+)-2-methyl-6[4-oxo-2-cyclohexen-1-yl]-2-(E)-heptenoic acid (4), a new nor-bisabolane derivative; (+)-(E)-exo-α-bergamoten-12-oic acid (5), a new bergamotene-type sesquiterpene; (2E,6E)-2,6-dimethyl-10-methylene-dodecatrienoic acid (6), a farnesene-type sesquiterpene reported for the first time ...
DOI:
10.1016/j.phytol.2021.01.004

10. Adaptive Laboratory Evolution for algal strain improvement: methodologies and applications

Author:
LaPanse, Alaina J.; Krishnan, Anagha; Posewitz, Matthew C.
Source:
Algal research 2021 v.53 pp. 102122
ISSN:
2211-9264
Subject:
strains,   etc   ; biotechnology; cells; evolution; experimental design; fungi; laboratories; microalgae; models; mutation; phenotype; stress tolerance;     Show all 12 Subjects
Abstract:
... Originally developed in bacterial and fungal models, Adaptive Laboratory Evolution (ALE) experimentally selects for targeted phenotypes by allowing cells containing beneficial random genetic mutations to replicate more quickly in the presence of environmental stress. When applied to microalgae, this technique has been used to increase growth rate, stress tolerance, substrate utilization, and produ ...
DOI:
10.1016/j.algal.2020.102122

11. Measuring strain in the exoskeleton of spiders—virtues and caveats

Author:
Blickhan, Reinhard; Weihmann, Tom; Barth, Friedrich G.
Source:
Journal of comparative physiology 2021 v.207 no.2 pp. 191-204
ISSN:
0340-7594
Subject:
strain gauges,   etc   ; body weight; compression strength; exoskeleton; foil; locomotion; metatarsus; quantitative analysis; tibia;     Show all 9 Subjects
Abstract:
... The measurement of cuticular strain during locomotion using foil strain gauges provides information both on the loads of the exoskeleton bears and the adaptive value of the specific location of natural strain detectors (slit sense organs). Here, we critically review available literature. In tethered animals, by applying loads to the metatarsus tip, strain and mechanical sensitivity (S = strain/loa ...
DOI:
10.1007/s00359-020-01458-y

12. Quorum Quenching in copper-tolerant Papiliotrema laurentii strains

Author:
Leguina, Ana Carolina del Valle; Fernández, Pablo Marcelo; Castellanos de Figueroa, Lucía I.; Nieto-Peñalver, Carlos Gabriel
Source:
Rhizosphere 2021 v.17 pp. 100298
ISSN:
2452-2198
Subject:
strains,   etc   ; alkalinization; copper; fungicides; homoserine; inactivation; quorum sensing; rhizosphere; tomatoes; yeasts;     Show all 10 Subjects
Abstract:
... In the rhizosphere, the role of yeasts in microbial interactions and signaling is an open question. To study the influence of fungicides on yeast inactivation of N-acyl homoserine lactone quorum sensing signals, we evaluated the resistance of Papiliotrema laurentii strains from tomato rhizosphere to CuSO₄ and Cu₂(OH)₃Cl fungicides, and the copper effect on yeast quorum quenching. Copper resistance ...
DOI:
10.1016/j.rhisph.2020.100298

13. Serological Approaches for Trypanosoma cruzi Strain Typing

Author:
Balouz, Virginia; Bracco, Leonel; Ricci, Alejandro D.; Romer, Guadalupe; Agüero, Fernán; Buscaglia, Carlos A.
Source:
Trends in parasitology 2021 v.37 no.3 pp. 214-225
ISSN:
1471-4922
Subject:
strains,   etc   ; Chagas disease; Trypanosoma cruzi; antibodies; antigens; cost effectiveness; genetic variation; genotyping; parasites; parasitology; population structure;     Show all 11 Subjects
Abstract:
... Trypanosoma cruzi, the protozoan agent of Chagas' disease, displays a complex population structure made up of multiple strains showing a diverse ecoepidemiological distribution. Parasite genetic variability may be associated with disease outcome, hence stressing the need to develop methods for T. cruzi typing in vivo. Serological typing methods that exploit the presence of host antibodies raised a ...
DOI:
10.1016/j.pt.2020.12.002

14. Unique polyacetylenic ester-neolignan derivatives from Mitrephora tomentosa and their antimalarial activities

Author:
Wongsomboon, Puttandon; Rattanajak, Roonglawan; Kamchonwongpaisan, Sumalee; Pyne, Stephen G.; Limtharakul, Thunwadee
Source:
Phytochemistry 2021 v.183 pp. 112615
ISSN:
0031-9422
Subject:
strains,   etc   ; Plasmodium falciparum; antimalarials; branches; cells; extracts; leaves; methanol; phytochemicals; spectral analysis; spectroscopy; toxicity;     Show all 12 Subjects
Abstract:
... The phytochemical investigation of the methanol extracts of the leaves and twigs of Mitrephora tomentosa Hook. f. & Thomson resulted in the isolation and identification of undescribed polyacetylenic ester-neolignan derivatives, along with six known compounds. These six undescribed natural products were named as mitrephentosins A-F. The structures of these compounds were determined by spectroscopic ...
DOI:
10.1016/j.phytochem.2020.112615

15. In vitro pharmacokinetics/pharmacodynamics of continuous ceftazidime infusion alone and in combination with colistin against Pseudomonas aeruginosa biofilm

Author:
Gómez-Junyent, Joan; Murillo, Oscar; Yu, Heidi H.; Azad, Mohammad A.K.; Wickremasinghe, Hasini; Rigo-Bonnin, Raul; Benavent, Eva; Ariza, Javier; Li, Jian
Source:
International journal of antimicrobial agents 2021 v.57 no.2 pp. 106246
ISSN:
0924-8579
Subject:
strains,   etc   ; Pseudomonas aeruginosa; antimicrobial properties; biofilm; ceftazidime; colistin; dosage; objectives; pharmacodynamics; pharmacokinetics;     Show all 10 Subjects
Abstract:
... Objectives: The pharmacokinetics/pharmacodynamics of continuous infusion (CI) beta-lactams for Pseudomonas aeruginosa biofilm infections has not been defined. This study evaluated the efficacy of several dosage regimens of CI ceftazidime, with or without colistin, an antibiotic with a potential antibiofilm effect, against biofilm-embedded P. aeruginosa.Methods: Mature biofilms of the reference str ...
DOI:
10.1016/j.ijantimicag.2020.106246

16. Variability in lag duration of Listeria monocytogenes strains in half Fraser enrichment broth after stress affects the detection efficacy using the ISO 11290-1 method

Author:
Bannenberg, Jasper W.; Abee, Tjakko; Zwietering, Marcel H.; den Besten, Heidy M.W.
Source:
International journal of food microbiology 2021 v.337 pp. 108914
ISSN:
0168-1605
Subject:
strain differences,   etc   ; Listeria monocytogenes; culture media; detection limit; food microbiology; heat stress; models; probability;     Show all 8 Subjects
Abstract:
... A collection of 23 Listeria monocytogenes strains of clinical and food origin was tested for their ability to recover and grow out in half Fraser enrichment broth following the ISO 11290-1:2017 protocol. Recovery of sub-lethally heat-injured cells in half Fraser broth was compared to reference cells with no stress pre-treatment. The enrichments were followed over time by plate counts and the growt ...
DOI:
10.1016/j.ijfoodmicro.2020.108914

17. Ultrasensitive microchip electrophoretic detection of the mecA gene in methicillin-resistant Staphylococcus aureus (MRSA) based on isothermal strand-displacement polymerase reaction

Author:
Lu, Yuqi; Luo, Feifei; Li, Zhi; Dai, Ge; Chu, Zhaohui; Zhang, Jingwen; Zhang, Fan; Wang, Qingjiang; He, Pingang
Source:
Talanta 2021 v.222 pp. 121686
ISSN:
0039-9140
Subject:
strains,   etc   ; DNA; detection limit; electrophoresis; genes; hospitals; infection; methicillin-resistant Staphylococcus aureus; microchip technology; pathogens; recycling;     Show all 11 Subjects
Abstract:
... Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main pathogens involved in hospital and community infection. To rapidly and sensitively detect the mecA gene, which is relevant to methicillin-resistant strains, microchip electrophoresis (MCE) integrated with isothermal strand-displacement polymerase reaction (ISDPR) was developed. In the ISDPR signal recycle amplification, the targ ...
DOI:
10.1016/j.talanta.2020.121686

18. Stacking Resistance Genes in Multiparental Interspecific Potato Hybrids to Anticipate Late Blight Outbreaks

Author:
Rogozina, Elena V.; Beketova, Mariya P.; Muratova, Oksana A.; Kuznetsova, Mariya A.; Khavkin, Emil E.
Source:
Agronomy 2021 v.11 no.1
ISSN:
2073-4395
Subject:
strains,   etc   ; Phytophthora infestans; Solanum; agronomy; evolution; germplasm; hybridization; hybrids; pathogens; pedigree; potatoes; resistance genes;     Show all 12 Subjects
Abstract:
... Stacking (pyramiding) several resistance genes of diverse race specificity in one and the same plant by hybridization provides for high and durable resistance to major diseases, such as potato late blight (LB), especially when breeders combine highly efficient genes for broad-spectrum resistance that are novel to the intruding pathogens. Our collection of potato hybrids manifesting long-lasting LB ...
DOI:
10.3390/agronomy11010115

19. Detection and phylogenetic analysis of porcine circovirus type 3 in Taiwan

Author:
Chang, Chia-Chun; Wu, Ching-Wei; Chang, Yu-Chih; Wu, Ching-Ying; Chien, Maw-Sheng; Huang, Chienjin
Source:
Archives of virology 2021 v.166 no.1 pp. 259-263
ISSN:
0304-8608
Subject:
strains,   etc   ; Porcine circovirus; Porcine circovirus-3; detection; nucleotide sequences; phylogeny; population; prevalence; swine; virology; Taiwan;     Show all 11 Subjects
Abstract:
... Porcine circovirus type 3 (PCV3) is a newly emerging porcine circovirus that infects pig populations worldwide. In this study, we investigated the prevalence of PCV3 in Taiwan and analyzed the phylogenetic relationships between the Taiwanese PCV3 strains and those from other countries. A total of 463 clinical specimens from sick pigs were collected in 2016–2019 and analyzed for PCV3 by PCR. The po ...
DOI:
10.1007/s00705-020-04870-6

20. Draft genome sequence of plastic degrading Bacillus sp. AIIW2 isolated from the Arabian ocean

Author:
Kumari, Alka; Bano, Nasreen; Chaudhary, Doongar R.; Jha, Bhavanath
Source:
Journal of basic microbiology 2021 v.61 no.1 pp. 37-44
ISSN:
0233-111X
Subject:
strains,   etc   ; Bacillus sonorensis; bioactive properties; biodegradation; bioremediation; carbon; environment; enzymes; exhibitions; genome; microorganisms; nucleotide sequences; plastics;     Show all 13 Subjects
Abstract:
... The endemic spread of plastic in the environment requires urgent need of a sustainable approach. Marine microbes found to have vast bioactivity and play a central role in biogeochemical cycling in the ocean; however, very few of them had been explored for biochemical cycling or plastic degradation. In the present study, we report the draft genome sequence of marine Bacillus sp. AIIW2 which was fou ...
DOI:
10.1002/jobm.202000416