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Van Tam Nguyen; Ki-hyun Cho; Nguyen Tuan Anh Mai; Jee-Yong Park; Thi Bich Ngoc Trinh; Min-Kyung Jang; Thi Thu Huyen Nguyen; Xuan Dang Vu; Thi Lan Nguyen; Van Diep Nguyen; Aruna Ambagala; Yong-Joo Kim; Van Phan Le
African swine fever; African swine fever virus; swine, etc ; genes; genotype; serotypes; virology; viruses; Vietnam; Show all 9 Subjects
Abstract:
... African swine fever (ASF) is a contagious and deadly viral disease affecting swine of all ages. ASF was first reported in Vietnam in February 2019, and it is now considered endemic in Vietnam. In this study, 122 ASF-positive samples collected from domestic pigs in 28 different provinces of northern, central, and southern Vietnam during outbreaks in 2019–2021 were genetically characterized. The fin ...
... African swine fever (ASF), caused by the African swine fever virus (ASFV), is a devastating disease of domestic and wild pigs. There is no effective vaccine, and the control of the disease relies mainly on surveillance and early detection of infected pigs. Previously, serological assays, such as ELISA, have been developed mainly based on recombinant structural viral proteins of ASFV, including p72 ...
... BACKGROUND: African swine fever (ASF) is a highly fatal swine disease, which threatens the global pig industry. There is no commercially available vaccine against ASF and effective subunit vaccines would represent a real breakthrough. METHODS: In this study, we expressed and purified two recombinant fusion proteins, OPM (OprI-p30-modified p54) and OPMT (OprI-p30-modified p54-T cell epitope), which ...
... African swine fever (ASF) is a major threat to pig production, and real-time PCR (qPCR) protocols are an integral part of ASF laboratory diagnosis. With the pandemic spread of ASF, commercial kits have risen on the market. In Germany, the kits have to go through an approval process and thus, general validation can be assumed. However, they have never been compared to each other. In this study, 12 ...
... Classical swine fever (CSF) is a worldwide devastating disease of the pig industry caused by classical swine fever virus (CSFV). In September 2018, an outbreak of CSF occurred in Japan where the disease had been eradicated and was officially designated a CSF‐free country since 2015. Following the detection of the first 2018 case on a farm in Gifu Prefecture, the disease spread among both farm pigs ...
... African swine fever (ASF) is an acute and highly contagious infectious disease caused by the African swine fever virus (ASFV). Currently, there is no vaccine against ASF worldwide, and no effective treatment measures are available. For this reason, developing a simple, rapid, specific, and sensitive serological detection method for ASFV antibodies is crucial for the prevention and control of ASF. ...
swine; swine dysentery, etc ; Brachyspira hyodysenteriae; databases; evolution; genetic variation; microbiology; multilocus sequence typing; population structure; Brazil; Show all 10 Subjects
Abstract:
... The aim of this study was to evaluate genetic diversity, distribution, evolution and population structure of Brazilian Brachyspira hyodysenteriae strains isolated from swine. Multilocus Sequence Typing (MLST) analysis using seven housekeeping genes was applied to 46 isolates obtained from outbreaks of swine dysentery that occurred between 2011 and 2015 in the states of Minas Gerais, São Paulo, Mat ...
... Farm animal welfare is an increasingly important issue, leading to the need for an efficient methodology to deliver accurate benchmarking. The “Real Welfare” project developed a methodology based on regular recording of a limited number of animal-based measures, so-called welfare outcomes, which allows faster and easier on-farm assessment of finishing pig welfare. The objective of this paper is to ...
... African swine fever (ASF) caused by African swine fever virus (ASFV) is becoming a serious threat to the swine industry worldwide. CD2v is a key pathogenic factor of ASFV and the protective antigen with low immunogenicity, whereas viral protein-based nanoparticles have advantages of precise assembly and high immunogenicity. In this study, the CD2v protein fused with Norovirus (NoV) P particle asse ...
Catherine Grace Elijah; Jessie D. Trujillo; Cassandra K. Jones; Taeyong Kwon; Charles R. Stark; Konner R. Cool; Chad B. Paulk; Natasha N. Gaudreault; Jason C. Woodworth; Igor Morozov; Carmina Gallardo; Jordan T. Gebhardt; Jürgen A. Richt
African swine fever virus; swine, etc ; DNA; biosafety; diet; feed contamination; feed mills; genes; genomics; research institutions; risk; Kansas; Show all 12 Subjects
Abstract:
... It is critical to have methods that can detect and mitigate the risk of African swine fever virus (ASFV) in potentially contaminated feed or ingredients bound for the United States. The purpose of this work was to evaluate feed batch sequencing as a mitigation technique for ASFV contamination in a feed mill, and to determine if a feed sampling method could identify ASFV following experimental inoc ...
... African swine fever (ASF) was first introduced into Northern China in 2018 and has spread through China since then. Here, we extracted the viral DNA from the blood samples from an ASF outbreak farm in Guangdong province, China and sequenced the whole genome. We assembled the full length genomic sequence of this strain, named China/GD/2019. The whole genome was 188,642 bp long (terminal inverted re ...
... The hypothesis that feed ingredients could serve as vehicles for the transport and transmission of viral pathogens was first validated under laboratory conditions. To bridge the gap from the laboratory to the field, this current project tested whether three significant viruses of swine could survive in feed ingredients during long‐distance commercial transport across the continental US. One‐metric ...
swine, etc ; blastomeres; embryogenesis; oocytes; sequence analysis; Show all 5 Subjects
Abstract:
... Long non‐coding RNAs (lncRNAs) function through multiple tiers of molecular circuits and are vital to gamete maturation and early embryo development. However, in pig early embryos, identification and expression dynamics of lncRNAs remain less studied. Here, we systematically analysed the expression dynamics of lncRNAs based on our previously published single‐cell RNA‐seq data from pig mature oocyt ...
swine; swine production, etc ; autocorrelation; biosecurity; disease control; disease occurrence; farms; phylogeny; porcine reproductive and respiratory syndrome; risk; virulence; viruses; Show all 12 Subjects
Abstract:
... Swine production in the United States is characterized by dynamic farm contacts through animal movements; such movements shape the risk of disease occurrence on farms. Pig movements have been linked to the spread of a virulent porcine reproductive and respiratory syndrome virus (PRRSV), RFLP type 1‐7‐4, herein denoted as phylogenetic sub‐lineage 1A [L1A]. This study aimed to quantify the contribut ...
... African swine fever virus (ASFV) is the causative agent of African swine fever (ASF). The virus causes an acute highly hemorrhagic disease in domestic pigs, with high mortality. Although the overall genome mutation rate of ASFV, a large DNA virus, is relatively low, ASFV exhibits genetic and antigenic diversity. ASFV can be classified into 24 genotypes on the basis of the B646L gene. Cross-protect ...
... The interactions of likely insect and murine vectors of the causative agent of swine dysentery, Brachyspira hyodysenteriae, were investigated. Insects were collected and analysed from 3 pig farms positive for B hyodysenteriae. Within these farms, several Musca domestica and Orphyra adult fly, Blatta sp. cockroach digestive tracts and hover fly (Eristalis sp) pupal form contents were positive in a ...
swine; swine production, etc ; animal physiology; average daily gain; body weight; diet; feed conversion; feed intake; ileum; lysine; occupations; protein composition; Show all 12 Subjects
Abstract:
... Slow‐growing pigs negatively affect production efficiency in conventional pig farms by increasing the occupation time of the facilities and being a limiting factor for the All‐In/All‐Out swine production systems. This subset of pigs is usually managed with the rest of the pigs, and their nutrient requirements may not be fulfilled. The purpose of the present study was to compare the productive perf ...
swine, etc ; Acinetobacter; bioaugmentation; biofilm; denitrification; nitrogen; wastewater; Show all 7 Subjects
Abstract:
... To enhance the performance of moving bed biofilm reactor (MBBR) inoculated with heterotrophic nitrification-aerobic denitrification (HN-AD) bacteria, bioaugmentation with Acinetobacter sp. TAC-1 was firstly employed and then the treatment performance for real swine wastewater was presented in this study. Results indicated that NH₄⁺-N and TN removal rates of bioaugmented reactor were significantly ...
swine, etc ; Aspergillus niger; cellulose; digestibility; fermentation; hemicellulose; Show all 6 Subjects
Abstract:
... The low fiber digestibility and unbalanced amino acids restricted the use of DDGS in swine diets. Key nutrition components dynamic monitoring and key regulatory pathways analysis were performed to find the rules of nutrition changes for DDGS fermented by Aspergillus niger. Cellulose and hemicellulose were reduced to 15.3% and 15.2%. 1,4-D-Xylobiose was decreased from 16.8 μg/mL to 0.2 μg/mL. Lys, ...
swine, etc ; agricultural policy; debt; risk; risk management; Show all 5 Subjects
Abstract:
... Little attention is given to cooperatives’ role in producer risk management. We review literature and theory in development of a hypothesized positive relationship between producers’ risk aversion and usage of cooperatives. We test the hypothesis using a sample of crop and hog producers and find evidence that cooperative membership and the percentage of commodity sold through cooperatives are posi ...