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1. Efficient production of recombinant secretory IgA against Clostridium difficile toxins in CHO-K1 cells

Author:
Bhaskara, Venugopal; Leal, Maria Trinidad; Seigner, Jacqueline; Friedrich, Theresa; Kreidl, Emanuel; Gadermaier, Elisabeth; Tesarz, Manfred; Rogalli, Azra; Stangl, Laura; Wallwitz, Jacqueline; Hammel, Katharina; Rothbauer, Mario; Moll, Herwig; Ertl, Peter; Hahn, Rainer; Himmler, Gottfried; Bauer, Anton; Casanova, Emilio
Source:
Journal of biotechnology 2021 v.331 pp. 1-13
ISSN:
0168-1656
Subject:
therapeutics,   etc   ; Clostridium difficile; biotechnology;     Show all 3 Subjects
Abstract:
... Despite the essential role secretory IgAs play in the defense against pathogenic invasion and the proposed value of recombinant secretory IgAs as novel therapeutics, currently there are no IgA-based therapies in clinics. Secretory IgAs are complex molecules and the major bottleneck limiting their therapeutic potential is a reliable recombinant production system. In this report, we addressed this i ...
DOI:
10.1016/j.jbiotec.2021.02.013

2. A comprehensive review on Morchella importuna: cultivation aspects, phytochemistry, and other significant applications

Author:
Sambyal, Krishika; Singh, Rahul Vikram
Source:
Folia microbiologica 2021 v.66 no.2 pp. 147-157
ISSN:
0015-5632
Subject:
therapeutics,   etc   ; Morchella; plant biochemistry;     Show all 3 Subjects
Abstract:
... Morchella importuna is one of the most highly priced edible mushrooms and a rich source of bioactive substances with numerous beneficial medicinal properties. It has been artificially cultivated in the last few years but due to the unclear mechanism of its fruiting body formation, the stable production has not been achieved yet. This review aims to provide the detailed information about the curren ...
DOI:
10.1007/s12223-020-00849-7

3. A guide to: generation and design of nanobodies

Author:
Muyldermans, Serge
Source:
TheFEBS journal 2021 v.288 no.7 pp. 2084-2102
ISSN:
1742-464X
Subject:
therapeutics,   etc   ; Camelidae; antigens; microorganisms;     Show all 4 Subjects
Abstract:
... A nanobody (Nb) is a registered trademark of Ablynx, referring to the single antigen‐binding domain of heavy chain‐only antibodies (HCAbs) that are circulating in Camelidae. Nbs are produced recombinantly in micro‐organisms and employed as research tools or for diagnostic and therapeutic applications. They were – and still are – also named single‐domain antibodies (sdAbs) or variable domain of the ...
DOI:
10.1111/febs.15515

4. It’s what’s on the inside that counts: Techniques for investigating the uptake and recycling of nanoparticles and proteins in cells

Author:
FitzGerald, Laura I.; Johnston, Angus P.R.
Source:
Journal of colloid and interface science 2021 v.587 pp. 64-78
ISSN:
0021-9797
Subject:
therapeutics,   etc   ; endocytosis; nanomedicine;     Show all 3 Subjects
Abstract:
... Many applications of nanomedicines depend on the therapeutic gaining access to the interior of cells. As most proteins and nanoparticles are taken up by endocytosis, determining the properties of nanoparticles that govern uptake is essential. In this review, we examine the current approaches for measuring the cellular uptake of nanoparticles and proteins. We explore the techniques distinguishing m ...
DOI:
10.1016/j.jcis.2020.11.076

5. Co-delivery of proteins and small molecule drugs for mitochondria-targeted combination therapy

Author:
Liew, Si Si; Zhou, Jia; Li, Lin; Yao, Shao Q.
Source:
Chemical communications 2021 v.57 no.26 pp. 3215-3218
ISSN:
1364-548X
Subject:
therapeutics,   etc   ; doxorubicin; nanocapsules; ribonucleases;     Show all 4 Subjects
Abstract:
... Herein, we report the first use of gluthathione (GSH)-responsive nanogel-based carriers for mitochondria-targeted delivery of functional proteins and antibodies. We further demonstrated the successful co-encapsulation of a protein and small molecule (RNase A/Doxorubicin) in dual-cargo nanocapsules for mitochondria-targeted combination therapy. ...
DOI:
10.1039/d0cc08020a

6. Foldamer-Based Potassium Channels with High Ion Selectivity and Transport Activity

Author:
Qi, Shuaiwei; Zhang, Chenyang; Yu, Hao; Zhang, Jing; Yan, Tengfei; Lin, Ze; Yang, Bing; Dong, Zeyuan
Source:
Journal of the American Chemical Society 2021 v.143 no.9 pp. 3284-3288
ISSN:
1520-5126
Subject:
therapeutics,   etc   ; humans; potassium;     Show all 3 Subjects
Abstract:
... Small molecules that independently perform natural channel-like functions show greatly potential in the treatment of human diseases. Taking advantage of aromatic helical scaffolds, we develop a kind of foldamer-based ion channels with lumen size varying from 3.8 to 2.3 Å through a sequence substitution strategy. Our results clearly elucidate the importance of channel size in ion transport selectiv ...
DOI:
10.1021/jacs.0c12128

7. Fighting microbial pathogens by integrating host ecosystem interactions and evolution

Author:
Burmeister, Alita R.; Hansen, Elsa; Cunningham, Jessica J.; Rego, E. Hesper; Turner, Paul E.; Weitz, Joshua S.; Hochberg, Michael E.
Source:
BioEssays 2021 v.43 no.3 pp. e2000272
ISSN:
0265-9247
Subject:
therapeutics,   etc   ; ecosystems; evolution; pathogenesis;     Show all 4 Subjects
Abstract:
... Successful therapies to combat microbial diseases and cancers require incorporating ecological and evolutionary principles. Drawing upon the fields of ecology and evolutionary biology, we present a systems‐based approach in which host and disease‐causing factors are considered as part of a complex network of interactions, analogous to studies of “classical” ecosystems. Centering this approach arou ...
DOI:
10.1002/bies.202000272

8. Mechanisms and therapeutic potential of interactions between human amyloids and viruses

Author:
Michiels, Emiel; Rousseau, Frederic; Schymkowitz, Joost
Source:
Cellular and molecular life sciences 2021 v.78 no.6 pp. 2485-2501
ISSN:
1420-682X
Subject:
therapeutics,   etc   ; amyloid; humans; pathogenicity;     Show all 4 Subjects
Abstract:
... The aggregation of specific proteins and their amyloid deposition in affected tissue in disease has been studied for decades assuming a sole pathogenic role of amyloids. It is now clear that amyloids can also encode important cellular functions, one of which involves the interaction potential of amyloids with microbial pathogens, including viruses. Human expressed amyloids have been shown to act b ...
DOI:
10.1007/s00018-020-03711-8

9. Mn–DNA coordination of nanoparticles for efficient chemodynamic therapy

Author:
Lin, Li; Yu, Jiantao; Lu, Hongwei; Wei, Zixiang; Chao, Zhicong; Wang, Zhongling; Wu, Weitao; Jiang, Hengfeng; Tian, Leilei
Source:
Chemical communications 2021 v.57 no.14 pp. 1734-1737
ISSN:
1364-548X
Subject:
therapeutics,   etc   ; DNA; toxicity;     Show all 3 Subjects
Abstract:
... A kind of nanoparticle is developed for highly efficient chemodynamic therapy that only relies on the endogenous H₂O₂ of cancer cells. For this nanoparticle, high-molecular-weight DNA is used as the biocompatible carrier to load abundant Mn²⁺ ions. Therefore, the resultant Mn–DNA coordination nanoparticles can efficiently deliver and sensitively release Mn²⁺ in cancer cells, resulting in high toxi ...
DOI:
10.1039/d0cc08191d

10. Flash Synthesis of Spherical Nucleic Acids with Record DNA Density

Author:
Hao, Yan; Li, Yanjuan; Song, Lei; Deng, Zhaoxiang
Source:
Journal of the American Chemical Society 2021 v.143 no.8 pp. 3065-3069
ISSN:
1520-5126
Subject:
therapeutics,   etc   ; DNA; butanol;     Show all 3 Subjects
Abstract:
... Nanoparticles (NPs) decorated with a high density of DNA strands, also known as spherical nucleic acids (SNAs), are widely used in DNA-programmable assembly, sensing, imaging, and therapeutics. A regular SNA synthesis is very time-consuming, which requires great caution to avoid NP aggregation. Herein we report an extremely simple, efficient, and scalable process to realize instant (in seconds) sy ...
DOI:
10.1021/jacs.1c00568

11. Comment on “Therapeutic target and molecular mechanism of vitamin C-treated pneumonia: a systematic study of network pharmacology” by R. Li, C. Guo, Y. Li, X. Liang, L. Yang and W. Huang, Food Funct., 2020, 11, 4765

Author:
Hemilä, Harri; Carr, Anitra
Source:
Food & function 2021 v.12 no.3 pp. 1371-1372
ISSN:
2042-650X
Subject:
therapeutics,   etc   ; pharmacology; pneumonia;     Show all 3 Subjects
Abstract:
... This Comment raises concerns about the article “Therapeutic target and molecular mechanism of vitamin C-treated pneumonia: a systematic study of network pharmacology”. ...
DOI:
10.1039/d0fo02189j

12. Predicting quality decay in continuously passaged mesenchymal stem cells by detecting morphological anomalies

Author:
Takemoto, Yuto; Imai, Yuta; Kanie, Kei; Kato, Ryuji
Source:
Journal of bioscience and bioengineering 2021 v.131 no.2 pp. 198-206
ISSN:
1389-1723
Subject:
therapeutics,   etc   ; cell culture; humans;     Show all 3 Subjects
Abstract:
... With rapid advances in cell therapy, technologies enabling both consistency and efficiency in cell manufacturing are becoming necessary. Morphological monitoring allows practical quality maintenance in cell manufacturing facilities, but relies heavily on human skill. For more reproducible and data-driven quality evaluation, image-based morphological analysis provides multiple advantages over manua ...
DOI:
10.1016/j.jbiosc.2020.09.022

13. The leucine‐rich repeat signaling scaffolds Shoc2 and Erbin: cellular mechanism and role in disease

Author:
Jang, HyeIn; Stevens, Payton; Gao, Tianyan; Galperin, Emilia
Source:
TheFEBS journal 2021 v.288 no.3 pp. 721-739
ISSN:
1742-464X
Subject:
therapeutics,   etc   ; biomarkers; humans;     Show all 3 Subjects
Abstract:
... Leucine‐rich repeat‐containing proteins (LRR proteins) are involved in supporting a large number of cellular functions. In this review, we summarize recent advancements in understanding functions of the LRR proteins as signaling scaffolds. In particular, we explore what we have learned about the mechanisms of action of the LRR scaffolds Shoc2 and Erbin and their roles in normal development and dis ...
DOI:
10.1111/febs.15450

14. Oral Vitamin D Therapy in Patients with Psoriasis

Author:
Stanescu, Ana Maria Alexandra; Simionescu, Anca Angela; Diaconu, Camelia Cristina
Source:
Nutrients 2021 v.13 no.1
ISSN:
2072-6643
Subject:
therapeutics,   etc   ; adjuvants; psoriasis;     Show all 3 Subjects
Abstract:
... Vitamin D treatment is effective when applied topically to the skin for plaque-type psoriasis. Oral vitamin D supplementation might be effective as an adjuvant treatment option in psoriasis. This umbrella review aimed to highlight the current knowledge regarding the use of oral vitamin D for treatment of patients with psoriasis. We performed a literature search and identified 107 eligible full-tex ...
DOI:
10.3390/nu13010163

15. Cancer and the breakdown of multicellularity: What Dictyostelium discoideum, a social amoeba, can teach us

Author:
Mathavarajah, Sabateeshan; VanIderstine, Carter; Dellaire, Graham; Huber, Robert J.
Source:
BioEssays 2021 v.43 no.4 pp. e2000156
ISSN:
0265-9247
Subject:
therapeutics,   etc   ; Dictyostelium discoideum; carcinogenesis; genes;     Show all 4 Subjects
Abstract:
... Ancient pathways promoting unicellularity and multicellularity are associated with cancer, the former being pro‐oncogenic and the latter acting to suppress oncogenesis. However, there are only a limited number of non‐vertebrate models for studying these pathways. Here, we review Dictyostelium discoideum and describe how it can be used to understand these gene networks. D. discoideum has a unicellu ...
DOI:
10.1002/bies.202000156

16. Reactive intermediates for interactome mapping

Author:
Seath, Ciaran P.; Trowbridge, Aaron D.; Muir, Tom W.; MacMillan, David W. C.
Source:
Chemical Society reviews 2021 v.50 no.5 pp. 2911-2926
ISSN:
1460-4744
Subject:
therapeutics,   etc   ; energy; physiology; precipitin tests;     Show all 4 Subjects
Abstract:
... The interactions of biomolecules underpin all cellular processes, and the understanding of their dynamic interplay can lead to significant advances in the treatment of disease through the identification of novel therapeutic strategies. Protein–protein interactions (PPIs) in particular play a vital role within this arena, providing the basis for the majority of cellular signalling pathways. Despite ...
DOI:
10.1039/d0cs01366h

17. IGF2BP1 is a targetable SRC/MAPK-dependent driver of invasive growth in ovarian cancer

Author:
Bley, Nadine; Schott, Annekatrin; Müller, Simon; Misiak, Danny; Lederer, Marcell; Fuchs, Tommy; Aßmann, Chris; Glaß, Markus; Ihling, Christian; Sinz, Andrea; Pazaitis, Nikolaos; Wickenhauser, Claudia; Vetter, Martina; Ungurs, Olga; Strauss, Hans-Georg; Thomssen, Christoph; Hüttelmaier, Stefan
Source:
RNA biology 2021 v.18 no.3 pp. 391-403
ISSN:
1555-8584
Subject:
therapeutics,   etc   ; ; RNA; mice; ovarian neoplasms;     Show all 5 Subjects
Abstract:
... Epithelial-to-mesenchymal transition (EMT) is a hallmark of aggressive, mesenchymal-like high-grade serous ovarian carcinoma (HGSOC). The SRC kinase is a key driver of cancer-associated EMT promoting adherens junction (AJ) disassembly by phosphorylation-driven internalization and degradation of AJ proteins. Here, we show that the IGF2 mRNA-binding protein 1 (IGF2BP1) is up-regulated in mesenchymal ...
DOI:
10.1080/15476286.2020.1812894

18. Ultrasensitive electrochemical assay for microRNA-21 based on CRISPR/Cas13a-assisted catalytic hairpin assembly

Author:
Cui, Ying; Fan, Shanji; Yuan, Ze; Song, Minghui; Hu, Jiawen; Qian, Dong; Zhen, Deshuai; Li, Junhua; Zhu, Baode
Source:
Talanta 2021 v.224 pp. 121878
ISSN:
0039-9140
Subject:
therapeutics,   etc   ; assays; biomarkers; electrochemistry; microRNA;     Show all 5 Subjects
Abstract:
... MicroRNAs (miRNAs) are related to many biological processes and regarded as biomarkers of disease. Rapid, sensitive, and specific methods for miRNA assay are very important for early disease diagnostic and therapy. In the present work, an ultrasensitive electrochemical biosensing platform has been developed for miRNA-21 assay by combining CRISPR-Cas13a system and catalytic hairpin assembly (CHA). ...
DOI:
10.1016/j.talanta.2020.121878

19. The Role of Genetic Factors in Endocrine Tissues Development and Its Regulation In Vivo and In Vitro

Author:
Goliusova, D. V.; Klementieva, N. V.; Panova, A. V.; Mokrysheva, N. G.; Kiselev, S. L.
Source:
Russian journal of genetics 2021 v.57 no.3 pp. 273-281
ISSN:
1022-7954
Subject:
therapeutics,   etc   ; embryogenesis; humans; medicine; mice;     Show all 5 Subjects
Abstract:
... Endocrine cells can be fully functional after heterotopic transplantation into mammals, which makes them a promising tool for regenerative medicine. However, to date, cellular therapy for endocrine diseases is limited by the inability to efficiently and stably obtain endocrine cells in vitro. The review focuses on current knowledge of the molecular genetic mechanisms involved in embryonic developm ...
DOI:
10.1134/S102279542103008X

20. Accounting for variation in and overuse of antibiotics among humans

Author:
Blaser, Martin J.; Melby, Melissa K.; Lock, Margaret; Nichter, Mark
Source:
BioEssays 2021 v.43 no.2 pp. e2000163
ISSN:
0265-9247
Subject:
therapeutics,   etc   ; Internet; antibiotics; caregivers; medicine;     Show all 5 Subjects
Abstract:
... Worldwide, antibiotic use is increasing, but many infections against which antibiotics are applied are not even caused by bacteria. Over‐the‐counter and internet sales preclude physician oversight. Regional differences, between and within countries highlight many potential factors influencing antibiotic use. Taking a systems perspective that considers pharmaceutical commodity chains, we examine an ...
DOI:
10.1002/bies.202000163