U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Subject "transcriptomics" Remove constraint Subject: "transcriptomics"
Start Over

Search

17,394  Search Results

« Previous | 1 - 20 of 17,394 |
Number of results to display per page

Search Results

1. Mepanipyrim induces visual developmental toxicity and vision-guided behavioral alteration in zebrafish larvae

Author:
Chao Shen; Yimei Cai; Jialing Li; Chengyong He; Zhenghong Zuo
Source:
Journal of environmental sciences (China) 2023 v.124 pp. 76-88
ISSN:
1001-0742
Subject:
transcriptomics,   etc   ; Danio rerio; anilinopyrimidine fungicides; aryl hydrocarbon receptors; developmental toxicity; fruit growing; fungi; genes; light intensity; mepanipyrim; opsin; phototransduction; quantitative polymerase chain reaction; wavelengths; China;     Show all 15 Subjects
Abstract:
... Mepanipyrim, an anilinopyrimidine fungicide, has been extensively used to prevent fungal diseases in fruit culture. Currently, research on mepanipyrim-induced toxicity in organisms is still very scarce, especially visual developmental toxicity. Here, zebrafish larvae were employed to investigate mepanipyrim-induced visual developmental toxicity. Intense light and monochromatic light stimuli-evoked ...
DOI:
10.1016/j.jes.2021.11.008

2. Mouse genomic and cellular annotations

Author:
Helen Long; Richard Reeves; Michelle M. Simon
Source:
Mammalian genome 2022 v.33 no.1 pp. 19-30
ISSN:
0938-8990
Subject:
transcriptomics,   etc   ; females; genes; genomics; humans; mice;     Show all 6 Subjects
Abstract:
... Mice have emerged as one of the most popular and valuable model organisms in the research of human biology. This is due to their genetic and physiological similarity to humans, short generation times, availability of genetically homologous inbred strains, and relatively easy laboratory maintenance. Therefore, following the release of the initial human reference genome, the generation of the mouse ...
DOI:
10.1007/s00335-021-09936-7

3. Single-cell transcriptomics reveals hidden information in trypanosomatids

Author:
Bryan E. Abuchery; Jennifer A. Black; Marcelo S. da Silva
Source:
Trends in parasitology 2022 v.38 no.1 pp. 4-6
ISSN:
1471-4922
Subject:
transcriptomics,   etc   ; Trypanosomatidae; gene expression; parasites; parasitology;     Show all 5 Subjects
Abstract:
... Dixenic parasites often encounter environmental extremes during the transition from vector to host. Preadapted transmission stages overcome these challenges to promote parasites' survival and ensure life cycle progression. Recently, Vigneron et al. and Briggs et al. used single-cell transcriptomics to investigate developmental stage specific gene expression patterns during parasite differentiation ...
DOI:
10.1016/j.pt.2021.10.006

4. A pyruvate-centered metabolic regulation mechanism for the enhanced expression of exogenous genes in Escherichia coli

Author:
Hongchen Zheng; Wenju Shu; Xiaoping Fu; Jiahan Wang; Yifan Yang; Jianyong Xu; Hui Song; Yanhe Ma
Source:
International journal of biological macromolecules 2022 v.203 pp. 58-66
ISSN:
0141-8130
Subject:
transcriptomics,   etc   ; Escherichia coli; acetates; byproducts; energy; metabolism;     Show all 6 Subjects
Abstract:
... A novel Escherichia coli efficient expression system had been constructed in our previous study. The system was based on the overexpression of endogenous genes prpD and malK to enhance the expression of exogenous genes. In this study, a general regulatory mechanism of prpD and malK was first revealed through transcriptome analysis and many experimental verifications. We surprisingly proved that ov ...
DOI:
10.1016/j.ijbiomac.2022.01.141

5. Bioengineered models of Parkinson’s disease using patient-derived dopaminergic neurons exhibit distinct biological profiles in a 3D microenvironment

Author:
Nicholas J. Fiore; Yosif M. Ganat; Kapil Devkota; Rebecca Batorsky; Ming Lei; Kyongbum Lee; Lenore J. Cowen; Gist Croft; Scott A. Noggle; Thomas J. F. Nieland; David L. Kaplan
Source:
Cellular and molecular life sciences 2022 v.79 no.2 pp. 78
ISSN:
1420-682X
Subject:
transcriptomics,   etc   ; dopamine; gene expression; humans; metabolites; neurons; silk;     Show all 7 Subjects
Abstract:
... Three-dimensional (3D) in vitro culture systems using human induced pluripotent stem cells (hiPSCs) are useful tools to model neurodegenerative disease biology in physiologically relevant microenvironments. Though many successful biomaterials-based 3D model systems have been established for other neurogenerative diseases, such as Alzheimer’s disease, relatively few exist for Parkinson’s disease (P ...
DOI:
10.1007/s00018-021-04047-7

6. Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins

Author:
Magdalena Malm; Chih-Chung Kuo; Mona Moradi Barzadd; Aman Mebrahtu; Num Wistbacka; Ronia Razavi; Anna-Luisa Volk; Magnus Lundqvist; David Kotol; Hanna Tegel; Sophia Hober; Fredrik Edfors; Torbjörn Gräslund; Veronique Chotteau; Ray Field; Paul G. Varley; Robert G. Roth; Nathan E. Lewis; Diane Hatton; Johan Rockberg
Source:
Metabolic engineering 2022 v.72 pp. 171-187
ISSN:
1096-7176
Subject:
transcriptomics,   etc   ; glycosylation; humans; recombinant proteins; secretion; therapeutics; transcriptome;     Show all 7 Subjects
Abstract:
... Biologics represent the fastest growing group of therapeutics, but many advanced recombinant protein moieties remain difficult to produce. Here, we identify metabolic engineering targets limiting expression of recombinant human proteins through a systems biology analysis of the transcriptomes of CHO and HEK293 during recombinant expression. In an expression comparison of 24 difficult to express pr ...
DOI:
10.1016/j.ymben.2022.03.009

7. Identification of new proteins in mature sieve elements

Author:
Niels Christian Sanden; Alexander Schulz
Source:
Physiologia plantarum 2022 v.174 no.1 pp. e13634
ISSN:
0031-9317
Subject:
transcriptomics,   etc   ; Arabidopsis; mass flow; sieve elements; streams; sugars;     Show all 6 Subjects
Abstract:
... The phloem enables vascular plants to transport photoassimilates from source tissues to heterotrophic sink tissues. In the phloem, unbroken strings of enucleated sieve elements, which lose the majority of their cellular contents upon maturation, provide a low resistance path for mass flow. The protein machinery in mature sieve elements performs vital functions to maintain the flow, transmit system ...
DOI:
10.1111/ppl.13634

8. Molecular insights into plant–microbe interactions for sustainable remediation of contaminated environment

Author:
Niraj R. Rane; Savita Tapase; Aakansha Kanojia; Anuprita Watharkar; El-Sayed Salama; Min Jang; Krishna Kumar Yadav; Mohammed A. Amin; Marina M.S. Cabral-Pinto; Jyoti P. Jadhav; Byong-Hun Jeon
Source:
Bioresource technology 2022 v.344 pp. 126246
ISSN:
0960-8524
Subject:
transcriptomics,   etc   ; endophytes; industrialization; metabolomics; phytoremediation; proteomics; rhizosphere; technology;     Show all 8 Subjects
Abstract:
... The widespread distribution of organic and inorganic pollutants in water resources have increased due to rapid industrialization. Rhizospheric zone-associated bacteria along with endophytic bacteria show a significant role in remediation of various pollutants. Metaomics technologies are gaining an advantage over traditional methods because of their capability to obtain detailed information on excl ...
DOI:
10.1016/j.biortech.2021.126246

9. Capturing the third dimension in drug discovery: Spatially-resolved tools for interrogation of complex 3D cell models

Author:
Daniel Simão; Catarina M. Gomes; Paula M. Alves; Catarina Brito
Source:
Biotechnology advances 2022 v.55 pp. 107883
ISSN:
0734-9750
Subject:
transcriptomics,   etc   ; biotechnology; drugs; human development; metabolomics; proteomics; spatial data;     Show all 7 Subjects
Abstract:
... Advanced three-dimensional (3D) cell models have proven to be capable of depicting architectural and microenvironmental features of several tissues. By providing data of higher physiological and pathophysiological relevance, 3D cell models have been contributing to a better understanding of human development, pathology onset and progression mechanisms, as well as for 3D cell-based assays for drug ...
DOI:
10.1016/j.biotechadv.2021.107883

10. From hybrid genomes to heterotic trait output: Challenges and opportunities

Author:
Wenwen Liu; Yilin Zhang; Hang He; Guangming He; Xing Wang Deng
Source:
Current opinion in plant biology 2022 v.66 pp. 102193
ISSN:
1369-5266
Subject:
transcriptomics,   etc   ; genome; genomics; heterosis; hybrids; plant biology; population genetics;     Show all 7 Subjects
Abstract:
... Heterosis (or hybrid vigor) has been widely used in crop seed breeding to improve many key economic traits. Nevertheless, the genetic and molecular basis of this important phenomenon has long remained elusive, constraining its flexible and effective exploitation. Advanced genomic approaches are efficient in characterizing the mechanism of heterosis. Here, we review how the omics approaches, includ ...
DOI:
10.1016/j.pbi.2022.102193

11. Identification of Key Proteins from the Alternative Lengthening of Telomeres-Associated Promyelocytic Leukemia Nuclear Bodies Pathway

Author:
Isaac Armendáriz-Castillo; Katherine Hidalgo-Fernández; Andy Pérez-Villa; Jennyfer M. García-Cárdenas; Andrés López-Cortés; Santiago Guerrero
Source:
Biology 2022 v.11 no.2 pp. -
ISSN:
2079-7737
Subject:
transcriptomics,   etc   ; genomics; leukemia; multiomics; neoplasm cells; proteomics; telomeres; therapeutics;     Show all 8 Subjects
Abstract:
... Alternative lengthening of telomeres-associated promyelocytic leukemia nuclear bodies (APBs) are a hallmark of telomere maintenance. In the last few years, APBs have been described as the main place where telomeric extension occurs in ALT-positive cancer cell lines. A different set of proteins have been associated with APBs function, however, the molecular mechanisms behind their assembly, colocal ...
DOI:
10.3390/biology11020185

12. Lingguizhugan decoction dynamically regulates MAPKs and AKT signaling pathways to retrogress the pathological progression of cardiac hypertrophy to heart failure

Author:
Yao Chen; Lin Li; Cunyu Hu; Xin Zhao; Peng Zhang; Yanxu Chang; Ye Shang; Yafen Pang; Weiqiang Qian; Xianzhe Qiu; Hongxia Zhang; Deqin Zhang; Shukun Zhang; Yuhong Li
Source:
Phytomedicine 2022 v.98 pp. 153951
ISSN:
0944-7113
Subject:
transcriptomics,   etc   ; drug therapy; heart failure; hypertrophy; morbidity; mortality; pharmacology;     Show all 7 Subjects
Abstract:
... Heart failure (HF) is a grave health concern, with high morbidity and mortality, calling for the urgent need for new and alternative pharmacotherapies. Lingguizhugan decoction (LD) is a classic Chinese formula clinically used to treat HF. However, the underlying mechanisms involved are not fully elucidated. Based on that, this study aims to investigate the effects and underlying mechanisms of LD o ...
DOI:
10.1016/j.phymed.2022.153951

13. Recent developments in systems biology and genetic engineering toward design of vaccines for TB

Author:
Sunita  ; Yogendra Singh; Gillian Beamer; Xingmin Sun; Pratyoosh Shukla
Source:
Critical reviews in biotechnology 2022 v.42 no.4 pp. 532-547
ISSN:
1549-7801
Subject:
transcriptomics,   etc   ; BCG vaccine; biotechnology; genomics; proteomics; tuberculosis; vaccine development;     Show all 7 Subjects
Abstract:
... Tuberculosis (TB) is one of the most prevalent diseases worldwide. The currently available Bacillus Calmette-Guérin vaccine is not sufficient in protecting against pulmonary TB. Although many vaccines have been evaluated in clinical trials, but none of them yet has proven to be more successful. Thus, new strategies are urgently needed to design more effective TB vaccines. The emergence of new tech ...
DOI:
10.1080/07388551.2021.1951649

14. Boosting the power of transcriptomics by developing an efficient gene expression profiling approach

Author:
Jing Wang; Jun Xu; Xiaohan Yang; Song Xu; Ming Zhang; Fei Lu
Source:
Plant biotechnology journal 2022 v.20 no.1 pp. 201-210
ISSN:
1467-7644
Subject:
transcriptomics,   etc   ; biotechnology; cost effectiveness; gene expression; genome; genomics; hexaploidy; wheat;     Show all 8 Subjects
Abstract:
... Recent advances in plant genomics are scaling up gene expression profiling from the individual level to the population level, making transcriptomics a more powerful tool while deciphering the genome function. This study developed an efficient 3′RNA‐seq method, Simplified Poly(A) Anchored Sequencing (SiPAS), to perform large‐scale experiments of gene expression quantification. Aside from being cost ...
DOI:
10.1111/pbi.13706

15. Molecular (co)evolution of hymenopteran social parasites and their hosts

Author:
Marah Stoldt; Maide Nesibe Macit; Erwann Collin; Susanne Foitzik
Source:
Current opinion in insect science 2022 v.50 pp. 100889
ISSN:
2214-5745
Subject:
transcriptomics,   etc   ; altruism; ancestry; epigenetics; genomics; selection pressure; social parasitism; virulence;     Show all 8 Subjects
Abstract:
... Social parasitism describes a fascinating way of life in which species exploit the altruistic behaviour of closely related, social species. Social parasites have repeatedly evolved in the social Hymenoptera, including ants, bees, and wasps. The common ancestry and shared (social) environment with their hosts facilitates the study of molecular adaptations to the parasitic lifestyle. Moreover, when ...
DOI:
10.1016/j.cois.2022.100889

16. Omics Approaches to Assess Flavor Development in Cheese

Author:
Rania Anastasiou; Maria Kazou; Marina Georgalaki; Anastasios Aktypis; Georgia Zoumpopoulou; Effie Tsakalidou
Source:
Foods 2022 v.11 no.2 pp. -
ISSN:
2304-8158
Subject:
transcriptomics,   etc   ; DNA; bioinformatics; cheeses; evolution; flavor; metabolites; metabolomics; microbiome; microorganisms;     Show all 10 Subjects
Abstract:
... Cheese is characterized by a rich and complex microbiota that plays a vital role during both production and ripening, contributing significantly to the safety, quality, and sensory characteristics of the final product. In this context, it is vital to explore the microbiota composition and understand its dynamics and evolution during cheese manufacturing and ripening. Application of high-throughput ...
DOI:
10.3390/foods11020188

17. Set up from the beginning: The origin and early development of cassava storage roots

Author:
Anna Vittoria Carluccio; Laure C. David; Joelle Claußen; Marco Sulley; Seun Raheemat Adeoti; Toyin Abdulsalam; Stefan Gerth; Samuel C. Zeeman; Andreas Gisel; Livia Stavolone
Source:
Plant, cell and environment 2022 v.45 no.6 pp. 1779-1795
ISSN:
0140-7791
Subject:
transcriptomics,   etc   ; cassava; computed tomography; early development; environment; secondary xylem; starch;     Show all 7 Subjects
Abstract:
... Despite the importance of storage root (SR) organs for cassava and the other root crops yield, their developmental origin is poorly understood. Here we use multiple approaches to shed light on the initial stages of root development demonstrating that SR and fibrous roots (FR) follow different rhizogenic processes. Transcriptome analysis carried out on roots collected before, during and after root ...
DOI:
10.1111/pce.14300

18. Spatial CRISPR genomics identifies regulators of the tumor microenvironment

Author:
Maxime Dhainaut; Samuel A. Rose; Guray Akturk; Aleksandra Wroblewska; Sebastian R. Nielsen; Eun Sook Park; Mark Buckup; Vladimir Roudko; Luisanna Pia; Robert Sweeney; Jessica Le Berichel; C. Matthias Wilk; Anela Bektesevic; Brian H. Lee; Nina Bhardwaj; Adeeb H. Rahman; Alessia Baccarini; Sacha Gnjatic; Dana Pe’er; Miriam Merad; Brian D. Brown
Source:
Cell 2022 v.185 no.7 pp. 1223-1239.e20
ISSN:
0092-8674
Subject:
transcriptomics,   etc   ; bioavailability; fibroblasts; genes; genomics; histopathology; immunosuppression; lung neoplasms; mice;     Show all 9 Subjects
Abstract:
... While CRISPR screens are helping uncover genes regulating many cell-intrinsic processes, existing approaches are suboptimal for identifying extracellular gene functions, particularly in the tissue context. Here, we developed an approach for spatial functional genomics called Perturb-map. We applied Perturb-map to knock out dozens of genes in parallel in a mouse model of lung cancer and simultaneou ...
DOI:
10.1016/j.cell.2022.02.015

19. Structure–Activity Relationships and Transcriptomic Analysis of Hypoxia-Inducible Factor Prolyl Hydroxylase Inhibitors

Author:
Andrey A. Poloznikov; Sergey V. Nikulin; Dmitry M. Hushpulian; Anna Yu. Khristichenko; Andrey I. Osipyants; Andrey F. Asachenko; Olga V. Shurupova; Svyatoslav S. Savin; Sue H. Lee; Irina N. Gaisina; Gregory R. J. Thatcher; Anthony Narciso; Eric P. Chang; Sergey V. Kazakov; Nancy Krucher; Vladimir I. Tishkov; Bobby Thomas; Irina G. Gazaryan
Source:
Antioxidants 2022 v.11 no.2 pp. -
ISSN:
2076-3921
Subject:
transcriptomics,   etc   ; computers; glycolysis; models; procollagen-proline dioxygenase; structure-activity relationships;     Show all 6 Subjects
Abstract:
... To evaluate the differences in action of commercially available 2-oxoglutarate mimetics and “branched-tail” oxyquinoline inhibitors of hypoxia-inducible factor prolyl hydroxylase (HIF PHD), the inhibitors’ IC₅₀ values in the activation of HIF1 ODD-luciferase reporter were selected for comparative transcriptomics. Structure–activity relationship and computer modeling for the oxyquinoline series of ...
DOI:
10.3390/antiox11020220

20. The de novo transcriptome identifies important zinc finger signatures associated with flowering in the orchid Arundina graminifolia

Author:
Sagheer Ahmad; Chuqiao Lu; Yonglu Wei; Jie Gao; Jianpeng Jin; Chuanyuan Zheng; Genfa Zhu; Fengxi Yang
Source:
Scientia horticulturae 2022 v.291 pp. 110572
ISSN:
0304-4238
Subject:
transcriptomics,   etc   ; Arundina; bamboos; transcription (genetics); transcriptome; vernalization; zinc finger motif;     Show all 7 Subjects
Abstract:
... Orchids take years to produce the most precious and expensive flowers. Continuous flowering is a rare characteristic of bamboo orchid (Arundina graminifolia). Therefore, finding genetic regulators of this flowering pattern can set new directions for other orchids. Present study demonstrates transcriptome analysis to identify a number of zinc finger transcription factors (ZF TFs) from five stages o ...
DOI:
10.1016/j.scienta.2021.110572