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1. Differential vs. comparative gel electrophoresis: New technology drives standardisation and quantification in protein two-dimensional gel electrophoresis

Author:
König, Simone
Source:
Trends in analytical chemistry 2020 v.122 pp. 115731
ISSN:
0165-9936
Subject:
two-dimensional gel electrophoresis,   etc   ; databases; fluorescence; gels; protein synthesis;     Show all 5 Subjects
Abstract:
... Two-dimensional gel electrophoresis (2DE) is a proven high-resolution separation technology in the protein laboratory. Nevertheless, ever since its invention the technique has had the drawback of low reproducibility. For protein expression analysis based on replicates, differential gel electrophoresis (DIGE) has, therefore, been invented. This technique is however not applicable for the comparison ...
DOI:
10.1016/j.trac.2019.115731

2. Proteomic screening identifies the direct targets of chrysin anti-lipid depot in adipocytes

Author:
Mei, Jie; Yang, Rong; Yang, Qiaohong; Wan, Wencheng; Wei, Xiaoyong
Source:
Journal of ethnopharmacology 2021 v.267 pp. 113361
ISSN:
0378-8741
Subject:
two-dimensional gel electrophoresis,   etc   ; Oriental traditional medicine; Oroxylum indicum; absorbance; adipocytes; adipose tissue; amino acids; chrysin; coenzyme A; computer simulation; fluorescence; fluorescence emission spectroscopy; gene expression; gene silencing; heat shock proteins; inflammation; liquid chromatography; mass spectrometry; metabolism; models; obesity; pathogenesis; proteomics; screening; transferases; triacylglycerols; ultraviolet-visible spectroscopy;     Show all 27 Subjects
Abstract:
... Overweight/obesity was mentioned by many countries as an obstacle to good health and long life, which increases risk of diseases and disorders. Previous studies suggested that the chronic low-grade inflammation present in the body was considered as the essential pathogenesis for obesity. Chrysin is extracted from traditional Chinese medicine Oroxylum indicum (Linn.) Kurz and plays a superior anti- ...
DOI:
10.1016/j.jep.2020.113361

3. Measuring quantitative proteomic distance between Spanish beef breeds

Author:
Rodríguez-Vázquez, R.; Mato, A.; López-Pedrouso, M.; Franco, D.; Sentandreu, M.A.; Zapata, C.
Source:
Food chemistry 2020 v.315 pp. 126293
ISSN:
0308-8146
Subject:
two-dimensional gel electrophoresis,   etc   ; Galician Blond; beef; biomarkers; breeding programs; cattle; longissimus muscle; meat tenderness; myosin; proteomics; tandem mass spectrometry;     Show all 11 Subjects
Abstract:
... Estimates of quantitative proteomic distance between populations have not been reported to date. Here, quantitative proteomic distances between three Spanish bovine breeds (Asturiana de los Valles, AV; Retinta, RE; and Rubia Gallega, RG) were estimated from two-dimensional electrophoresis profiles of meat samples of longissimus thoracis muscle at 2 h post-mortem. Statistically significant distance ...
DOI:
10.1016/j.foodchem.2020.126293

4. Comparison of protein extraction methods for 2DE-based proteomic analysis of duckweed Spirodela polyrhiza, a small aquatic model plant

Author:
Wang, Yibo; Wang, Xiuyun; Yang, Ruixue; Niu, Liangjie; Wang, Wei
Source:
Aquatic botany 2020 v.163 pp. 103216
ISSN:
0304-3770
Subject:
two-dimensional gel electrophoresis,   etc   ; Spirodela polyrhiza; acetone; aquatic plants; models; phenol; plant biology; proteins; proteomics; trichloroacetic acid; trichloroacetic acid precipitation;     Show all 11 Subjects
Abstract:
... Duckweed, a small floating aquatic plant in the family Lemnaceae, has considerable potential for agricultural and environmental applications. Proteomic analysis will facilitate the study of plant biology and plant-environment interactions. A reliable and reproducible protein extraction method is critical for successful proteomic analysis. To date, there have been no evaluation studies of extractio ...
DOI:
10.1016/j.aquabot.2020.103216

5. The evolution of two-dimensional gel electrophoresis - from proteomics to emerging alternative applications

Author:
Lee, Pey Yee; Saraygord-Afshari, Neda; Low, Teck Yew
Source:
Journal of chromatography 2020 v.1615 pp. 460763
ISSN:
0021-9673
Subject:
two-dimensional gel electrophoresis,   etc   ; chromatography; isoelectric point; mass spectrometry; molecular weight; protein isoforms; protein synthesis; proteins; proteome; proteomics;     Show all 10 Subjects
Abstract:
... Two-dimensional gel electrophoresis (2-DE) is a technique that has been widely applied in a variety of proteomics studies. It is capable of resolving complex protein mixtures into individual protein spots based on their isoelectric point and molecular weight, enabling large-scale analysis of protein expression patterns for deciphering their changes in different biological conditions. 2-DE is a pow ...
DOI:
10.1016/j.chroma.2019.460763

6. Inventory of proteoforms as a current challenge of proteomics: Some technical aspects

Author:
Naryzhny, Stanislav
Source:
Journal of proteomics 2019 v.191 pp. 22-28
ISSN:
1874-3919
Subject:
two-dimensional gel electrophoresis,   etc   ; genes; humans; mass spectrometry; proteome; proteomics;     Show all 6 Subjects
Abstract:
... The main intricacy in the human proteome is that it is tremendously complex and composed from diverse and heterogeneous gene products. These products are called protein species or proteoforms and are the smallest units of the proteome. In pursuit of the comprehensive profiling of the human proteome, significant advances in the technology of so called “Top-Down” mass spectrometry based proteomics, ...
DOI:
10.1016/j.jprot.2018.05.008

7. Proteomic analysis of differentially abundant proteins in the embryo and endosperm of Givotia moluccana (L.) Sreem

Author:
Pullaiahgari, Durgeshwar; Meena, Rajesh Kumar; Tirupaati, Swaroopa Rani; Gudipalli, Padmaja
Source:
Journal of forestry research 2020 v.31 no.6 pp. 2097-2109
ISSN:
1007-662X
Subject:
two-dimensional gel electrophoresis,   etc   ; Angiospermae; biogenesis; cell growth; chloroplasts; desorption; embryo (plant); endosperm; energy; fatty acids; germination; metabolism; metalloproteinases; proteins; proteome; proteomics; stress response; trees; zinc;     Show all 19 Subjects
Abstract:
... Proteome analysis of embryo and endosperm of Givotia moluccana, a promising angiosperm tree species was carried out using two-dimensional electrophoresis combined with matrix-assisted laser desorption/ionization time of flight to identify differentially abundant proteins and their possible functions. The study revealed the presence of 492 protein spots in the embryo and 432 in the endosperm, and t ...
DOI:
10.1007/s11676-019-00995-w

8. Proteomic analysis of the response of Rhizopus oryzae ENHE to pentachlorophenol: Understanding the mechanisms for tolerance and degradation of this toxic compound

Author:
Ruiz-Lara, A.; Fierro, F.; Carrasco, U.; Oria, J.A.; Tomasini, A.
Source:
Process biochemistry 2020 v.95 pp. 242-250
ISSN:
1359-5113
Subject:
two-dimensional gel electrophoresis,   etc   ; Rhizopus oryzae; broths; energy metabolism; fungi; liquid chromatography; mass spectrometry; methyltransferases; pentachlorophenol; peroxidases; proteins; proteome; proteomics; stress response; toxic substances;     Show all 15 Subjects
Abstract:
... Pentachlorophenol (PCP) is a toxic compound widely distributed in the environment. Rhizopus oryzae ENHE has been shown to possess PCP degradation capacities, peroxidases and phenoloxidases are expected to be involved in the degradation process but no studies have been performed that confirm this hypothesis. We have carried out a proteomic study aiming to understand the mechanisms by which R. oryze ...
DOI:
10.1016/j.procbio.2020.02.016

9. Assessment of changes in the content of sulforaphane and expression levels of CYP79F1 and myrosinase genes and proteomic profile of Lepidium draba plant under water-deficit stress induced by polyethylene glycol

Author:
Jamshidi Goharrizi, Kiarash; Fatehi, Foad; Nazari, Maryam; Salehi, Fatemeh; Maleki, Mahmood
Source:
Acta physiologiae plantarum 2020 v.42 no.6 pp. 101
ISSN:
0137-5881
Subject:
two-dimensional gel electrophoresis,   etc   ; Lepidium draba; anthocyanins; biosynthesis; carotenoids; chlorophyll; genes; glutamic acid; medicinal plants; phenols; polyethylene glycol; proteomics; secondary metabolites; sulforaphane; thioglucosidase; transcription factors; water stress; weeds;     Show all 18 Subjects
Abstract:
... Lepidium draba weed, which has a very valuable secondary metabolite named sulforaphane, is a precious medicinal plant that very few studies have been done on it. In this study, 5-day-old Lepidium draba plants were grown for 9 days in differentially levels of polyethylene glycol and the amount of total Phenols, total Flavonoids, total Anthocyanins, sulforaphane, chlorophyll and Carotenoids as well ...
DOI:
10.1007/s11738-020-03085-1

10. Identification of 60 secreted proteins for Mycoplasma bovis with secretome assay

Author:
Zubair, Muhammad; Muhamed, Shafii Abdullahi; Khan, Farhan Anwar; Zhao, Gang; Menghwar, Harish; Faisal, Muhammad; Zhang, Hui; Zhu, Xifang; Rasheed, Muhammad Asif; Chen, Yingyu; Marawan, Marawan A.; Chen, Huanchun; Guo, Aizhen
Source:
Microbial pathogenesis 2020 v.143 pp. 104135
ISSN:
0882-4010
Subject:
two-dimensional gel electrophoresis,   etc   ; ABC transporters; Mycoplasma bovis; bioinformatics; bovine mastitis; cattle; desorption; genome; matrix-assisted laser desorption-ionization mass spectrometry; pathogenesis; pathogens; pneumonia; protein secretion; vaccines; virulence;     Show all 15 Subjects
Abstract:
... Mycoplasma bovis is a risky pathogen mainly responsible for pneumonia and mastitis in cattle. Up to date, its pathogenesis is not clear. Since secreted proteins have a tricky role in M. bovis pathogenesis, this study was designed to systematically reveal M. bovis secretome and potential role in virulence of the pathogen. By using bioinformatics tools, a total of 246 secreted proteins were predicte ...
DOI:
10.1016/j.micpath.2020.104135

11. Phenotypic and allelic variation for wort protein Z in Australian and Canadian barleys

Author:
Luo, Hao; Zhang, Qisen; Tucek, Mark; Zhang, Xiao-Qi; Li, Chengdao
Source:
Journal of cereal science 2020 v.93 pp. 102935
ISSN:
0733-5210
Subject:
two-dimensional gel electrophoresis,   etc   ; alleles; allelic variation; amino acid sequences; barley; beers; foams; genetic markers; genotype; liquid chromatography; marker-assisted selection; mass spectrometry; phenotype; protein content; proteins; soaking;     Show all 16 Subjects
Abstract:
... Protein Z is a major component in beer foam. Two-dimensional electrophoresis was used to analyze wort proteins of two Australian (Buloke and Commander) and two Canadian (CDC Meredith and Bentley) varieties. The Canadian barley contained more abundant proteins from MW 40–45 kDa (pI 5 to 7). These proteins were identified as either protein Z4 or protein Z7 using liquid chromatography–mass spectromet ...
DOI:
10.1016/j.jcs.2020.102935

12. Systemic Amyloid A Amyloidosis in Stejneger’s Beaked Whales (Mesoplodon stejnegeri)

Author:
Nakagun, Shotaro; Watanabe, Kenichi; Tajima, Yuko; Yamada, Tadasu K.; Kobayashi, Yoshiyasu
Source:
Veterinary pathology 2020 v.57 no.3 pp. 437-444
ISSN:
1544-2217
Subject:
two-dimensional gel electrophoresis,   etc   ; Western blotting; Ziphiidae; amyloid; amyloidosis; animal pathology; antiserum; body condition; disease incidence; eosin; histopathology; immunohistochemistry; inflammation; intestines; liver; monitoring; peptides; stomach; tissues; whales; Japan;     Show all 21 Subjects
Abstract:
... Stejneger’s beaked whales (Mesoplodon stejnegeri) are one of the lesser known species of mammals, with little information available on their population status or incidence of diseases. Recent pathologic investigations on stranded and bycaught wild cetaceans around Hokkaido, Japan, revealed an unusually high incidence of systemic amyloidosis in this species, warranting further investigation. The ob ...
DOI:
10.1177/0300985820914079

13. Study of proteins with mercury in fish from the Amazon region

Author:
Cavecci-Mendonça, Bruna; Cavalcante de Souza Vieira, José; Monteiro de Lima, Paula; Leite, Aline Lima; Buzalaf, Marília Afonso Rabelo; Zara, Luiz Fabrício; de Magalhães Padilha, Pedro
Source:
Food chemistry 2020 v.309 pp. 125460
ISSN:
0308-8146
Subject:
two-dimensional gel electrophoresis,   etc   ; atomic absorption spectrometry; bioinformatics; electrospray ionization mass spectrometry; fish; fractionation; hydroelectric power; mercury; power plants; proteins; rivers; triose-phosphate isomerase; Amazonia;     Show all 13 Subjects
Abstract:
... The high concentrations of mercury found in Amazon have been intensively studied by the scientific community in the last decades. These mercurial species bind preferentially to proteins. Therefore, this work proposal sought to obtain the fractionation, identification and study of mercury – bound proteins present in samples of muscular and hepatic tissue from fish collected in the reservoir of the ...
DOI:
10.1016/j.foodchem.2019.125460

14. Site-Specific and Quantitative N-Glycan Heterogeneity Analysis of the Charge Isomers of an Anti-VEGF Recombinant Fusion Protein by High-Resolution Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Author:
Li, Xiaoyu; He, Qing; Yu, Zixiang; Xie, Yuping; Zhao, Xinyuan; Huang, Yi; Wang, Yongzhong; Ke, Xiao; Qian, Xiaohong; Dong, Junxing; Ying, Wantao
Source:
Analytical chemistry 2020 v.92 no.8 pp. 5695-5700
ISSN:
1520-6882
Subject:
two-dimensional gel electrophoresis,   etc   ; biopharmaceuticals; gels; glycopeptides; glycosylation; ions; isomers; macular degeneration; mass spectrometry; pH; quality control; quantitative analysis; recombinant fusion proteins;     Show all 13 Subjects
Abstract:
... Glycan modification prompts important concerns about the quality control of biopharmaceutical production. Conbercept is a multiglycosylated recombinant fusion protein drug approved for the treatment of age-related macular degeneration (AMD). With 14 N-glycosites in the molecule and 7 N-glycosites in the monomer, the charge isomer separation and characterization of conbercept pose great challenges ...
DOI:
10.1021/acs.analchem.0c00592

15. Effects of Fructose and Overexpression of Shock-Related Gene groL on Plantaricin Q7 Production

Author:
Zhang, Jianming; Han, Xue; Zhang, Lanwei; Yi, Huaxi; Chen, Shiwei; Gong, Pimin
Source:
Probiotics and antimicrobial proteins 2020 v.12 no.1 pp. 32-38
ISSN:
1867-1306
Subject:
two-dimensional gel electrophoresis,   etc   ; Lactobacillus plantarum; antimicrobial peptides; biosynthesis; fructose; gene overexpression; genes; plantaricins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction;     Show all 10 Subjects
Abstract:
... The effects of fructose on plantaricin Q7 biosynthesis in Lactobacillus plantarum (L. plantarum) Q7 were investigated. Fructose induced and accelerated plantaricin Q7 production in the early stages of biosynthesis. Two-dimensional electrophoresis and quantitative real-time reverse transcriptase PCR (qRT-PCR) revealed that shock-related proteins were overexpressed in L. plantarum grown in MRS-fruct ...
DOI:
10.1007/s12602-019-09537-6

16. Proteome changes in lamb semimembranosus muscles associated with the inclusion of sunflower cake in their diet

Author:
de Melo, Aline Moreira Portela; López‐Pedrouso, María; Costa, Roberto Germano; Franco, Daniel; de Alencar Araripe Noronha Moura, Arlindo; da Silva, Taciane Alves; Moreno, Frederico Bruno Mendes Batista; de Lima Júnior, Valdi; de Oliveira Monteiro‐Moreira, Ana Cristina; de Medeiros, Ariosvaldo Nunes; de Azevedo Moreira, Renato; Lorenzo, Jose M.
Source:
International journal of food science & technology 2020 v.55 no.3 pp. 995-1001
ISSN:
0950-5423
Subject:
two-dimensional gel electrophoresis,   etc   ; biodiesel; biomarkers; diet; gels; industrial byproducts; lambs; malate dehydrogenase; males; mass spectrometry; meat quality; muscles; phosphopyruvate hydratase; proteome; structural proteins; sunflower cake;     Show all 16 Subjects
Abstract:
... A diet based on sunflower cake for lambs was assayed in order to reuse biodiesel industrial by‐products with the aim of reducing livestock costs and evaluating their influence on meat quality. To achieve these goals, sixteen male lambs were fed diets containing different levels of sunflower cake (control, 5%, 10% and 15%). Afterwards, their semimembranosus muscles were analysed by two‐dimensional ...
DOI:
10.1111/ijfs.14328

17. Proteomic analysis reveals dynamic regulation of fruit ripening in response to exogenous ethylene in kiwifruit cultivars

Author:
Shin, Mi Hee; Muneer, Sowbiya; Kim, Yun-Hee; Lee, Jeung Joo; Bae, Dong Won; Kwack, Yong-Bum; Kumarihami, H. M. Prathibhani C.; Kim, Jin Gook
Source:
Horticulture, environment and biotechnology 2020 v.61 no.1 pp. 93-114
ISSN:
2211-3452
Subject:
two-dimensional gel electrophoresis,   etc   ; Actinidia deliciosa; Calvin cycle; bioinformatics; ethylene; fruit quality; gene expression regulation; kiwifruit; protein content; protein synthesis; proteins; proteomics; ripening;     Show all 13 Subjects
Abstract:
... Understanding the fruit ripening mechanism is critical for fruit quality improvement. Although postharvest ethylene application is known to enhance the onset of fruit ripening, the exact mechanisms remain unclear. In this study, a gel-based proteomic analysis was performed to investigate the changes in protein profiles during the ripening of exogenous-ethylene-treated kiwifruit (Actinidia delicios ...
DOI:
10.1007/s13580-019-00209-6

18. Exploiting the reference genome sequence of hexaploid wheat: a proteomic study of flour proteins from the cultivar Chinese Spring

Author:
Altenbach, Susan B.; Chang, Han-Chang; Simon-Buss, Annamaria; Mohr, Toni; Huo, Naxin; Gu, Yong Q.
Source:
Functional & integrative genomics 2020 v.20 no.1 pp. 1-16
ISSN:
1438-793X
Subject:
two-dimensional gel electrophoresis,   etc   ; Triticum; allergenicity; cultivars; economic valuation; endosperm; genes; gliadin; gluten; glutenins; hexaploidy; nucleotide sequences; protein content; proteomics; sequence diversity; tandem mass spectrometry; wheat; wheat flour;     Show all 18 Subjects
Abstract:
... Although the economic value of wheat flour is determined by the complement of gluten proteins, these proteins have been challenging to study because of the complexity of the major protein groups and the tremendous sequence diversity among wheat cultivars. The completion of a high-quality wheat genome sequence from the reference wheat Chinese Spring recently facilitated the assembly and annotation ...
DOI:
10.1007/s10142-019-00694-z
PubMed:
31250230
PubMed Central:
PMC6954139

19. Proteomic and microscopic approaches in understanding mechanisms of shell-loosening of shrimp (Pandalus borealis) induced by high pressure and protease

Author:
Dang, Tem Thi; Jessen, Flemming; Martens, Helle Juel; Gringer, Nina; Olsen, Karsten; Bøknæs, Niels; Orlien, Vibeke
Source:
Food chemistry 2019 v.289 pp. 729-738
ISSN:
0308-8146
Subject:
two-dimensional gel electrophoresis,   etc   ; Pandalus borealis; collagen; microscopy; molecular weight; peeling; proteinases; proteomics; shrimp;     Show all 9 Subjects
Abstract:
... Shell-loosening is of importance in facilitating shrimp peeling. In this study, enzyme and high pressure (HP) improved the shell-loosening at different degrees, which were observed as gaps by microscopy. The shell-loosening gap induced by an endoprotease with broad specificity (Endocut-03L, 53 μm) was much higher than that induced by HP at 100 MPa (HP100, 12 μm), followed by an endoprotease with h ...
DOI:
10.1016/j.foodchem.2019.03.059

20. Refinement of two-dimensional electrophoresis for vitreous proteome profiling using an artificial neural network

Author:
Santos, Fátima M.; Albuquerque, Tânia; Gaspar, Leonor M.; Dias, João M. L.; Castro e Sousa, João P.; Paradela, Alberto; Tomaz, Cândida T.; Passarinha, Luís A.
Source:
Analytical and bioanalytical chemistry 2019 v.411 no.20 pp. 5115-5126
ISSN:
1618-2642
Subject:
two-dimensional gel electrophoresis,   etc   ; experimental design; gels; neural networks; proteins; proteome; solubilization; temperature;     Show all 8 Subjects
Abstract:
... Despite technological advances, two-dimensional electrophoresis (2DE) of biological fluids, such as vitreous, remains a major challenge. In this study, artificial neural network was applied to optimize the recovery of vitreous proteins and its detection by 2DE analysis through the combination of several solubilizing agents (CHAPS, Genapol, DTT, IPG buffer), temperature, and total voltage. The high ...
DOI:
10.1007/s00216-019-01887-y