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1. Separation methods for milk proteins on polyacrylamide gel electrophoresis: Critical analysis and options for better resolution

Author:
Neelima Sharma; Rajan Sharma; Yudhishthir S. Rajput; Bimlesh Mann; Richa Singh; Kamal Gandhi
Source:
International dairy journal 2021 v.114 pp. 104920
ISSN:
0958-6946
Subject:
two-dimensional gel electrophoresis,   etc   ; milk; polyacrylamide gel electrophoresis; sulfates; whey;     Show all 5 Subjects
Abstract:
... Over the years, a number of methodological variations in polyacrylamide gel electrophoresis (PAGE) have been attempted for the separation of milk proteins. Caseins are similar in size and differ very little in net charge from each other. Also, in heated milk, κ-casein not only complexes with whey proteins but also undergoes self-polymerisation. These cause problems in the resolution of milk protei ...
DOI:
10.1016/j.idairyj.2020.104920

2. Calreticulin as a special marker to distinguish dental pulp stem cells from gingival mesenchymal stem cells

Author:
Tong Lei; Jian Wang; Yanyan Liu; Peng Chen; Zhihui Zhang; Xiaoshuang Zhang; Xiao Wang; Quanhai Li; Hongwu Du
Source:
International journal of biological macromolecules 2021 v.178 pp. 229-239
ISSN:
0141-8130
Subject:
two-dimensional gel electrophoresis,   etc   ; angiogenesis; calreticulin; dissociation; inflammation; protein content; proteomics; tooth pulp;     Show all 8 Subjects
Abstract:
... proteomics and two-dimensional gel electrophoresis technology were used in combination to describe the protein profile of DPSCs and GMSCs extracted from the same donor. A total of 2821 proteins were identified by LC-MS/MS, of which 248 differentially abundant proteins (DAPs) were highly expressed in GMSCs while 782 proteins were highly expressed in DPSCs. The biological functions and molecular pathways ...
DOI:
10.1016/j.ijbiomac.2021.02.126

3. Hevea brasiliensis latex proteomics: a review of analytical methods and the way forward

Author:
Mohd Afiq Hazlami Habib; Mohd Nazri Ismail
Source:
Journal of plant research 2021 v.134 no.1 pp. 43-53
ISSN:
0918-9440
Subject:
two-dimensional gel electrophoresis,   etc   ; Hevea brasiliensis; biosynthesis; latex; mass spectrometry; proteomics; research; rubber; stress tolerance;     Show all 9 Subjects
Abstract:
... tolerance. However, the exact mechanisms of the pathways are still not clear. Proteomic analyses on latex have found various proteins and revealed how they fit into the mechanisms of the biological pathways. In the past three decades, there has been rapid latex protein identification due to the improvement of latex protein extraction methods, as well as the emergence of two-dimensional gel electrophoresis ...
DOI:
10.1007/s10265-020-01231-x

4. Proteomics-Based Identification of Salivary Changes in Patients with Burning Mouth Syndrome

Author:
Candela Castillo-Felipe; Lorena Franco-Martínez; Asta Tvarijonaviciute; Pia Lopez-Jornet; Elsa Lamy
Source:
Biology 2021 v.10 no.5 pp. -
ISSN:
2079-7737
Subject:
two-dimensional gel electrophoresis,   etc   ; anxiety; elastase; etiology; inflammation; leukocytes; mouth; pathophysiology; proteomics; saliva; taste;     Show all 11 Subjects
Abstract:
... Burning mouth syndrome (BMS) is a chronic oral condition characterized by an intraoral burning sensation, taste alterations, and dry mouth sensations. Although a number of factors have been closely related to the appearance of the symptoms, including anxiety, depression, and sleep disturbances, the etiology of BMS remains unclear. Furthermore, currently no objective diagnostic tools exist, making ...
DOI:
10.3390/biology10050392

5. Effects of Three-Month Administration of High-Saturated Fat Diet and High-Polyunsaturated Fat Diets with Different Linoleic Acid (LA, C18:2n–6) to α-Linolenic Acid (ALA, C18:3n–3) Ratio on the Mouse Liver Proteome

Author:
Kamila P. Liput; Adam Lepczyński; Agata Nawrocka; Ewa Poławska; Magdalena Ogłuszka; Aneta Jończy; Weronika Grzybek; Michał Liput; Agnieszka Szostak; Paweł Urbański; Agnieszka Roszczyk; Chandra S. Pareek; Mariusz Pierzchała
Source:
Nutrients 2021 v.13 no.5 pp. -
ISSN:
2072-6643
Subject:
two-dimensional gel electrophoresis,   etc   ; carbohydrate metabolism; cholesterol; glucose; high fat diet; linoleic acid; liver; mice; pH; proteome; proteomics;     Show all 11 Subjects
Abstract:
... :1 (5:1 group). After three months of diets, liver proteins were resolved by two-dimensional gel electrophoresis (2DE) using 17 cm non-linear 3–10 pH gradient strips. Protein spots with different expression were identified by MALDI-TOF/TOF. The expression of 13 liver proteins was changed in the SFA group compared to the STD group (↓: ALB, APOA1, IVD, MAT1A, OAT and PHB; ↑: ALDH1L1, UniProtKB—Q91V76 ...
DOI:
10.3390/nu13051678

6. Proteomic screening for the identification of proteins involved in resistance to Xanthomonas campestris pv. malvacearum in cotton

Author:
Ivonaldo Reis Santos; Thuanny Borba Rios; Mariana Rocha Maximiano; Wirton Macedo Coutinho; Liziane Maria De Lima; Luciano Paulino Silva; Osmundo Brilhante Oliveira-Neto; Angela Mehta
Source:
Physiological and molecular plant pathology 2021 v.113 pp. 101562
ISSN:
0885-5765
Subject:
two-dimensional gel electrophoresis,   etc   ; Gossypium hirsutum; Xanthomonas campestris pv. malvacearum; blight; cotton; photosynthesis; plant pathology; proteomics;     Show all 8 Subjects
Abstract:
... In this study, we identified proteins in cotton associated with resistance to bacterial blight (BB) by using two-dimensional electrophoresis (2-DE). To search for proteins that may trigger resistance/defense response, here we analyzed Gossypium hirsutum plants at 24 and 120 h after inoculation with Xanthomonas campestris pv. malvacearum (Xcm). Photosynthesis-related proteins showed pronounced redu ...
DOI:
10.1016/j.pmpp.2020.101562

7. The Senescence-Associated Endonuclease, PhENDO1, Is Upregulated by Ethylene and Phosphorus Deficiency in Petunia

Author:
Michelle L. Jones; Shuangyi Bai; Yiyun Lin; Laura J. Chapin
Source:
Horticulturae 2021 v.7 no.3 pp. -
ISSN:
2311-7524
Subject:
two-dimensional gel electrophoresis,   etc   ; DNA; Petunia; RNA; amino acid sequences; catabolism; ethylene; genes; longevity; pH; phosphorus; viruses;     Show all 12 Subjects
Abstract:
... The upregulation of endonuclease activities and subsequent decreases in the nucleic acid content of leaves and petals are characteristics of senescence that allow for nutrient remobilization from dying organs. We previously identified a 43-kDa endonuclease activity (PhNUC1) that was upregulated in Petunia × hybrida petals during senescence. PhNUC1 has optimal activity at neutral pH, is enhanced by ...
DOI:
10.3390/horticulturae7030046

8. Comparative proteomics analyses of mycelial, conidial, and Secreted Proteins of high-pathogenic and weak-pathogenic Fusarium oxysporum f. sp. cucumerinum isolates

Author:
Eman Elagamey; Magdi A.E. Abdellatef; Arunima Sinha; Said M. Kamel
Source:
Physiological and molecular plant pathology 2021 v.115 pp. 101675
ISSN:
0885-5765
Subject:
two-dimensional gel electrophoresis,   etc   ; Fusarium oxysporum; conidia; crop quality; cucumbers; disease severity; metabolism; mycelium; pathogenesis; plant pathology; polypeptides; proteomics; virulence;     Show all 13 Subjects
Abstract:
... the pathogenicity and virulence variation of FOC isolates, a comprehensive proteomic study was done to compare the polypeptide patterns of mycelial, conidial, and secreted proteins of the highly pathogenic (hp-FOC) and weakly pathogenic (wp-FOC) isolates. Two-dimensional gel electrophoresis (2-DE) coupled with LC-MS/MS analysis led to the identification of shared and unique proteins involved in metabolism ...
DOI:
10.1016/j.pmpp.2021.101675

9. Protein mass spectrometry reveals lycorine exerting anti-multiple-myeloma effect by acting on VDAC2 and causing mitochondrial abnormalities

Author:
Hui Yi; Xiaokai Shen; Haiqin Wang; Saiqun Luo; Wancheng Guo; Peng Chen; Lei Hu; Long Liang; Yanfei Gong; Xiaojuan Xiao; Jing Liu
Source:
Biotechnology letters 2021 v.43 no.3 pp. 537-546
ISSN:
0141-5492
Subject:
two-dimensional gel electrophoresis,   etc   ; Western blotting; biotechnology; gene expression regulation; lycorine; membrane potential; mitochondria; mitochondrial membrane; myeloma; proteomics;     Show all 10 Subjects
Abstract:
... OBJECTIVE: Two-dimensional electrophoresis (2-DE) and MALDI-TOF/TOF mass spectrometry were performed to compare the proteomic alterations of lycorine-treated and control cells to further investigate the anti-multiple myeloma (MM) mechanisms of lycorine. RESULTS: Mass spectrometry results showed that after lycorine treatment of MM cells, 42% of the differentially expressed proteins had subcellular ...
DOI:
10.1007/s10529-020-03053-2

10. Proteome responses of Rhizobium tropici CIAT 899 upon apigenin and salt stress induction

Author:
M.R. Maximiano; E. Megías; I.R. Santos; L.S. Santos; F.J. Ollero; M. Megías; O.L. Franco; A. Mehta
Source:
Applied soil ecology 2021 v.159 pp. 103815
ISSN:
0929-1393
Subject:
two-dimensional gel electrophoresis,   etc   ; ABC transporters; Fabaceae; Rhizobium tropici; apigenin; bacteria; energy; nitrogen; nodulation; proteome; rhizosphere; salt stress; symbiosis;     Show all 13 Subjects
Abstract:
... Rhizobium tropici establishes symbiosis with several leguminous species, inducing nitrogen fixing nodules. Communication between rhizobia and legumes starts when the flavonoids secreted by plant roots interact with the R. tropici NodD protein to recognize plant signaling molecules present in the rhizosphere and begin the nodulation process. Some studies in R. tropici CIAT 899 have demonstrated tha ...
DOI:
10.1016/j.apsoil.2020.103815

11. Wheat (Triticum aestivum L.) TaHMW1D Transcript Variants Are Highly Expressed in Response to Heat Stress and in Grains Located in Distal Part of the Spike

Author:
Chan Seop Ko; Jin-Baek Kim; Min Jeong Hong; Yong Weon Seo
Source:
Plants 2021 v.10 no.4 pp. -
ISSN:
2223-7747
Subject:
two-dimensional gel electrophoresis,   etc   ; Triticum aestivum; cultivars; exons; flowering; glutamine; glutenins; grain yield; heat stress; polypeptides; seed storage; transcription (genetics); valine; wheat;     Show all 14 Subjects
Abstract:
... High-temperature stress during the grain filling stage has a deleterious effect on grain yield and end-use quality. Plants undergo various transcriptional events of protein complexity as defensive responses to various stressors. The “Keumgang” wheat cultivar was subjected to high-temperature stress for 6 and 10 days beginning 9 days after anthesis, then two-dimensional gel electrophoresis (2DE ...
DOI:
10.3390/plants10040687

12. Comparing mRNA expression and protein abundance in MDR Mycobacterium tuberculosis: Novel protein candidates, Rv0443, Rv0379 and Rv0147 as TB potential diagnostic or therapeutic targets

Author:
Alireza Hadizadeh Tasbiti; Shamsi Yari; Seyed Davar Siadat; Morteza Karimipoor; Farzad Badmasti; Morteza Masoumi; Farid Abdolrahimi; Sharareh Khanipour; Seyed Mehdi Hassanzadeh; Mostafa Ghalami Nobar; Fatemeh Yari
Source:
Biotechnology reports 2021 v.30 pp. e00641
ISSN:
2215-017X
Subject:
two-dimensional gel electrophoresis,   etc   ; Mycobacterium tuberculosis; T-lymphocytes; ammonium sulfate; biotechnology; epitopes; gene expression; mass spectrometry; prediction; protein composition; public health; therapeutics; tuberculosis;     Show all 13 Subjects
Abstract:
... Tuberculosis (TB) is a sizable public health threat in the world. This study was conducted to determine the differential protein composition between susceptible and MDRTB strains. Tuberculosis proteins were extracted by Triton™ X-114 and ammonium sulfate. Two-dimensional gel electrophoresis protein spots were selected for identification by mass spectrometry and mRNA expression levels were ...
DOI:
10.1016/j.btre.2021.e00641

13. The first analysis of Trichinella spiralis and Trichinella britovi adult worm excretory-secretory proteins by two-dimensional electrophoresis coupled with LC-MS/MS

Author:
Sylwia Grzelak; Anna Stachyra; Justyna Bień-Kalinowska
Source:
Veterinary parasitology 2021 v.297 pp. 109096
ISSN:
0304-4017
Subject:
two-dimensional gel electrophoresis,   etc   ; Trichinella britovi; Trichinella spiralis; adults; gene ontology; isomerases; meat; muscles; parasites; proteolysis; proteome; trichinosis; veterinary parasitology;     Show all 13 Subjects
Abstract:
... The two most common Trichinella species present in European countries are Trichinella spiralis and Trichinella britovi. The consumption of raw or undercooked meat with invasive larvae results in trichinellosis. Currently, the most commonly used sources for detecting specific anti-Trichinella antibodies is ELISA with the muscle larvae (ML) excretory-secretory (E-S) proteins. However, these serologi ...
DOI:
10.1016/j.vetpar.2020.109096

14. A comparative protein profile of accessory glands of virgin and mated Leucinodes orbonalis males

Author:
Saraswathi Saraswathi; Chaitra Bodampalli Sarvesh; Tannavi Kiran; Mamtha Ravindran; Sowrabha Ramachandra; Karthik V. Rao; Manjulakumari Doddamane
Source:
Physiological entomology 2021 v.46 no.1 pp. 60-69
ISSN:
0307-6962
Subject:
two-dimensional gel electrophoresis,   etc   ; Leucinodes orbonalis; databases; desorption; eggplants; entomology; females; hexamerins; ionization; lipids; males; pests; physiology; protein composition; proteolysis; proteomics; spectroscopy; spermatozoa;     Show all 18 Subjects
Abstract:
... , respectively, using two‐dimensional gel electrophoresis (2D‐GE). Among the identified spots, 49 are unique to the virgin, whereas 118 are unique to mated MAGs, with 68 spots found to be common. The differentially expressed MAG proteins, 14 are up‐regulated, and 16 are down‐regulated in virgin after mating. We used matrix‐assisted laser desorption ionization (MALDI)–mass spectrometry (MS) to identify the 13 ...
DOI:
10.1111/phen.12345

15. Differential proteomics reveals main determinants for the improved pectinase activity in UV-mutagenized Aspergillus niger strain

Author:
Weiling Lin; Xiaohong Xu; Ruirui Lv; Wei Huang; Hafeez ul Haq; Yuanyuan Gao; Hongli Ren; Canhua Lan; Baoyu Tian
Source:
Biotechnology letters 2021 v.43 no.4 pp. 909-918
ISSN:
0141-5492
Subject:
two-dimensional gel electrophoresis,   etc   ; Aspergillus niger; DNA-directed RNA polymerase; alpha-N-arabinofuranosidase; biotechnology; mutants; pectate lyase; pectinesterase; pectins; polygalacturonase; proteomics;     Show all 11 Subjects
Abstract:
... OBJECTIVES: To reveal the potential mechanism and key determinants that contributed to the improved pectinase activity in Aspergillus niger mutant EIMU2, which was previously obtained by UV-mutagenesis from the wild-type A. niger EIM-6. RESULTS: Proteomic analysis for Aspergillus niger EIMU2 by two-dimensional electrophoresis demonstrated that mutant EIMU2 harbored a multiple enzyme system for the ...
DOI:
10.1007/s10529-020-03075-w

16. Impact of ethylene glycol on DHEA dihydroxylation in Colletotrichum lini: Increasing the expression of cytochrome P450 and 6-phosphogluconate dehydrogenase and enhancing the generation of NADPH

Author:
Hui Li; Weili Shi; Cong Li; Xiaomei Zhang; Jinsong Gong; Jinsong Shi; Mattheos A.G. Koffas; Zhenghong Xu
Source:
Biochemical engineering journal 2021 v.166 pp. 107860
ISSN:
1369-703X
Subject:
two-dimensional gel electrophoresis,   etc   ; Colletotrichum; biotransformation; contraceptives; cytochrome P-450; dihydroxylation; ethylene glycol; intermediate product; pH; phosphogluconate dehydrogenase; prasterone; proteomics; solvents;     Show all 13 Subjects
Abstract:
... 3β,7α,15α-trihydroxy-5-androsten-17-one (7α,15α-diOH-DHEA), a key precursor of the novel oral contraceptive “Yasmin”, can be obtained by dihydroxylation in the 7α and 15α positions of dehydroepiandrosterone (DHEA) by Colletotrichum lini CGMCC 6051. However, the commercial-scale application of 7α,15α-diOH-DHEA is limited by the poor dihydroxylation efficiency and its low yield. This research invest ...
DOI:
10.1016/j.bej.2020.107860

17. The evolution of two-dimensional gel electrophoresis - from proteomics to emerging alternative applications

Author:
Pey Yee Lee; Neda Saraygord-Afshari; Teck Yew Low
Source:
Journal of chromatography 2020 v.1615 pp. 460763
ISSN:
0021-9673
Subject:
two-dimensional gel electrophoresis,   etc   ; chromatography; isoelectric point; mass spectrometry; molecular weight; protein isoforms; protein synthesis; proteins; proteome; proteomics;     Show all 10 Subjects
Abstract:
... Two-dimensional gel electrophoresis (2-DE) is a technique that has been widely applied in a variety of proteomics studies. It is capable of resolving complex protein mixtures into individual protein spots based on their isoelectric point and molecular weight, enabling large-scale analysis of protein expression patterns for deciphering their changes in different biological conditions. 2-DE ...
DOI:
10.1016/j.chroma.2019.460763

18. Proteomic approach to identify the differentially abundant proteins during flavour development in tuberous roots of Decalepis hamiltonii Wight & Arn.

Author:
Kiran Kamireddy; Priyanka Purushottam Sonbarse; Shashank. K. Mishra; Lalit Agrawal; Puneet S. Chauhan; Charu Lata; Giridhar Parvatam
Source:
3 Biotech 2021 v.11 no.4 pp. 173
ISSN:
2190-572X
Subject:
two-dimensional gel electrophoresis,   etc   ; adenosinetriphosphatase; benzaldehyde; biochemical pathways; bioenergy; biosynthesis; cell division; constitutional isomers; flavor; homeostasis; methyltransferases; plant growth; proteomics; vacuoles; vanillin;     Show all 15 Subjects
Abstract:
... 2-Hydroxy-4-Methoxy Benzaldehyde (2H4MB) is a structural isomer of vanillin produced in the tuberous roots of D. hamiltonii. Both vanillin and 2H4MB share the common phenylpropanoid pathway for their synthesis. Unlike vanillin, in which the biosynthetic pathway was well elucidated in V. planifolia, the 2H4MB biosynthetic pathway is not known in any of its plant sources. To find the key enzymes/pro ...
DOI:
10.1007/s13205-021-02714-x

19. Differential vs. comparative gel electrophoresis: New technology drives standardisation and quantification in protein two-dimensional gel electrophoresis

Author:
Simone König
Source:
Trends in analytical chemistry 2020 v.122 pp. 115731
ISSN:
0165-9936
Subject:
two-dimensional gel electrophoresis,   etc   ; databases; fluorescence; gels; protein synthesis;     Show all 5 Subjects
Abstract:
... Two-dimensional gel electrophoresis (2DE) is a proven high-resolution separation technology in the protein laboratory. Nevertheless, ever since its invention the technique has had the drawback of low reproducibility. For protein expression analysis based on replicates, differential gel electrophoresis (DIGE) has, therefore, been invented. This technique is however not applicable ...
DOI:
10.1016/j.trac.2019.115731

20. Proteomic profile of human stem cells from dental pulp and periodontal ligament

Author:
Tong Lei; Jian Wang; Yanyan Liu; Peng Chen; Zhihui Zhang; Xiaoshuang Zhang; Wenhuan Guo; Xiao Wang; Quanhai Li; Hongwu Du
Source:
Journal of proteomics 2021 v.245 pp. 104280
ISSN:
1874-3919
Subject:
two-dimensional gel electrophoresis,   etc   ; biomarkers; cell adhesion; cell proliferation; energy transfer; gels; gene expression; humans; ligaments; mitochondria; physiological transport; proteomics; secretion; tooth pulp;     Show all 14 Subjects
Abstract:
... BackgroundThe study of molecular profiling of dental pulp stem cells (DPSCs) and periodontal ligament stem cells (PDLSCs) contributes to understanding the high proliferation ability and multi-lineage differentiation potential.ObjectivesThe aim of the study was to compare the protein abundance and specific markers of DPSCs and PDLSCs by protein profiles.Material and methodsThe DPSCs and PDLSCs extr ...
DOI:
10.1016/j.jprot.2021.104280