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1. Quantification of hepatitis E virus in raw pork livers using droplet digital RT-PCR

Author:
Kairui Wang; Libing Liu; Jinfeng Wang; Xiaoxia Sun; Qingan Han; Cang Zhou; Xiangdong Xu; Jianchang Wang
Source:
Food microbiology 2023 v.109 pp. 104114
ISSN:
0740-0020
Subject:
viral load,   etc   ; Orthohepevirus A; RNA; biosafety; detection limit; droplets; etiological agents; food microbiology; genes; hepatitis E; livestock; phylogeny; pork; reverse transcription; viruses; China;     Show all 16 Subjects
Abstract:
... Hepatitis E virus (HEV) is the causative agent of hepatitis E. Some of the rise in hepatitis E infection in China may be linked to undercooked pork. In this study, we established a reverse transcription droplet digital PCR (RT-ddPCR) method to detect HEV in raw pork livers. The detection limit of the assay for HEV RNA was as low as 1.81 copies/μL. The suggested approach was validated on 14 samples ...
DOI:
10.1016/j.fm.2022.104114

2. Approaches to purification and concentration of rAAV vectors for gene therapy

Author:
Lyubava Belova; Konstantin Kochergin‐Nikitsky; Anastasia Erofeeva; Alexander Lavrov; Svetlana Smirnikhina
Source:
BioEssays 2022 v.44 no.6 pp. e2200019
ISSN:
0265-9247
Subject:
viral load,   etc   ; algorithms; gene therapy;     Show all 3 Subjects
Abstract:
... Recombinant adeno‐associated viruses (rAAVs) are promising vectors for the delivery of various genetic constructs into eukaryotic cells. rAAVs have a number of properties that make it possible to successfully use them both in vitro and in vivo. Purification and concentration of rAAV vectors are critical for achieving high viral titer, stability, efficiency, and purity. This review systematically a ...
DOI:
10.1002/bies.202200019

3. A multi-center study comparing the analytical sensitivity and clinical concordance of three HIV-1 viral load assays

Author:
Sergio Carmona; Lucia Hans; Merlin Njoya; Christian O. Simon; Ed G. Marins; Véronique Michel-Treil; Philip Cunningham
Source:
Journal of virological methods 2022 v.300 pp. 114373
ISSN:
0166-0934
Subject:
viral load,   etc   ; detection limit; standard deviation;     Show all 3 Subjects
Abstract:
... HIV-1 viral load assays are essential tools for clinical management of people living with HIV-1.We evaluated concordance between three assays: the cobas® HIV-1 test for use on the cobas® 6800 and cobas® 8800 systems (cobas HIV-1); the COBAS® TaqMan® HIV-1 Test, v2.0 for use with the High Pure System and the COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 test, v2.0. Analytical sensitivity, precision and acc ...
DOI:
10.1016/j.jviromet.2021.114373

4. The performance of human cytomegalovirus digital PCR reference measurement procedure in seven external quality assessment schemes over four years

Author:
Mojca Milavec; Jernej Pavšič; Alexandra Bogožalec Košir; Gerwyn M. Jones; Denise M. O'Sullivan; Alison S. Devonshire; Fran Van Heuverswyn; Maria Karczmarczyk; Jannika Neeb; Annabell Plauth; Philippe Corbisier; Heinz Schimmel; Andreas Kummrow; Jörg Neukammer; Carole A. Foy; Martin Kammel; Hans-Peter Grunert; Heinz Zeichhardt; Jim F. Huggett
Source:
Methods 2022 v.201 pp. 65-73
ISSN:
1046-2023
Subject:
viral load,   etc   ; Human betaherpesvirus 5; calibration; genes;     Show all 4 Subjects
Abstract:
... A candidate digital PCR (dPCR)-based reference measurement procedure for quantification of human cytomegalovirus (hCMV) was evaluated in 10 viral load comparison schemes (seven external quality assessment (EQA) and three additional training schemes) organized by INSTAND e.V. over four years (between September 2014 and March 2018). Four metrology institutes participated in these schemes using the s ...
DOI:
10.1016/j.ymeth.2021.03.016

5. Seroreversion of IgG anti‐HEV in HIV cirrhotic patients: A long‐term multi‐sampling longitudinal study

Author:
Pedro López‐López; Mario Frias; Angela Camacho; Isabel Machuca; Javier Caballero‐Gómez; María A. Risalde; Ignacio García‐Bocanegra; Ignacio Pérez‐Valero; Jose C. Gomez‐Villamandos; Antonio Rivero‐Juárez; Antonio Rivero
Source:
Transboundary and emerging diseases 2022 v.69 no.5 pp. e1541
ISSN:
1865-1674
Subject:
viral load,   etc   ; antibodies; longitudinal studies; retrospective studies; seroprevalence;     Show all 5 Subjects
Abstract:
... The aim of our study was to evaluate HEV antibody kinetics in HIV/HCV‐coinfected patients with cirrhosis. A longitudinal retrospective study was designed. Patients were followed up every 6 months; anti‐HEV IgG and IgM antibodies levels and HEV‐RNA by qPCR were analysed. The prevalence and incidence of every HEV infection marker were calculated. The kinetics of anti‐HEV IgG and IgM during the follo ...
DOI:
10.1111/tbed.14486

6. Comparison of the efficacy, safety and durability of a switch to co-formulated RPV/TDF-TAF/FTC or DTG/ABC/3TC in virologically-suppressed HIV-1-infected patients in a single Italian centre: a cohort data analysis

Author:
Filippo Lagi; Annarita Botta; Seble Tekle Kiros; Massimo Meli; Beatrice Borchi; Annalisa Cavallo; Marco Pozzi; Alessandro Bartoloni; Gaetana Sterrantino
Source:
International journal of antimicrobial agents 2022 v.59 no.1 pp. 106465
ISSN:
0924-8579
Subject:
viral load,   etc   ; HIV infections; diarrhea; durability; nausea; risk; toxicity;     Show all 7 Subjects
Abstract:
... This study evaluated the efficacy, safety and durability of a switch to co-formulated RPV/TDF-TAF/FTC (RPV-STR) or DTG/ABC/3TC (DTG-STR) in virologically-suppressed HIV-positive patients in a single Italian centre. All HIV-infected ART-experienced patients switching to RPV-STR or DTG-STR with HIV-RNA <50 copies/mL were included. Outcomes were incidence rate and rate ratios for discontinuation due ...
DOI:
10.1016/j.ijantimicag.2021.106465

7. Epidemic Characteristics of HIV Drug Resistance in Hefei, Anhui Province

Author:
Shan Zheng; Jianjun Wu; Jingjing Hao; Dong Wang; Zhongwang Hu; Lei Liu; Chang Song; Jing Hu; Yanhua Lei; Hai Wang; Lingjie Liao; Yi Feng; Yiming Shao; Yuhua Ruan; Hui Xing
Source:
Pathogens 2022 v.11 no.8 pp. -
ISSN:
2076-0817
Subject:
viral load,   etc   ; cross-sectional studies; databases; drug resistance; China;     Show all 5 Subjects
Abstract:
... To study the characteristics of HIV pretreatment drug resistance (PDR) and acquired drug resistance (ADR) in Hefei, a cross-sectional survey was used to collect 816 samples from newly reported HIV infections from 2017 to 2020 and 127 samples from HIV infections with virological failure from 2018 to 2019 in Hefei. HIV drug resistance levels and drug resistance mutations were interpreted using the S ...
DOI:
10.3390/pathogens11080866

8. Evaluation of filter paper to transport oro/nasopharyngeal samples to detect SARS-CoV-2 by RT-qPCR

Author:
Maiara dos Santos Carneiro; Fabiana Caroline Zempulski Volpato; Priscila Lamb Wink; Dariane Castro Pereira; Luciana Giordani; Afonso Luís Barth
Source:
Journal of virological methods 2022 v.308 pp. 114587
ISSN:
0166-0934
Subject:
viral load,   etc   ; RNA; Severe acute respiratory syndrome coronavirus 2;     Show all 3 Subjects
Abstract:
... To evaluate filter paper as a means to transport oro/nasopharyngeal samples from laboratories with few resources for SARS-CoV-2 detection by RT-qPCR in a central laboratory that usually performs this technique as routine. A total of 40 specimens were evaluated in parallel by RT-qPCR carried out after RNA extraction using two different protocols: direct RNA extraction (Protocol A - reference method ...
DOI:
10.1016/j.jviromet.2022.114587

9. COS-7 and SVGp12 Cellular Models to Study JCPyV Replication and MicroRNA Expression after Infection with Archetypal and Rearranged-NCCR Viral Strains

Author:
Carla Prezioso; Sara Passerini; Dolores Limongi; Anna Teresa Palamara; Ugo Moens; Valeria Pietropaolo
Source:
Viruses 2022 v.14 no.9 pp. -
ISSN:
1999-4915
Subject:
viral load,   etc   ; DNA; Polyomaviridae; exosomes; microRNA; transcription (genetics); virus replication;     Show all 7 Subjects
Abstract:
... Since the non-coding control region (NCCR) and microRNA (miRNA) could represent two different and independent modalities of regulating JC polyomavirus (JCPyV) replication at the transcriptional and post-transcriptional levels, the interplay between JC viral load based on NCCR architecture and miRNA levels, following JCPyV infection with archetypal and rearranged (rr)-NCCR JCPyV variants, was explo ...
DOI:
10.3390/v14092070

10. Community-Based Screening for Hepatitis B and C Infectivity Using Two Quantitative Antigens to Identify Endemic Townships

Author:
Wei-Cheng Huang; Yu-Chen Lin; Po-Ju Chen; Nien-Tzu Hsu; Chia-Ling Tu; Te-Sheng Chang; Chao-Hung Hung; Kwong-Ming Kee; Wen-Hua Chao; Sheng-Nan Lu
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
viral load,   etc   ; chronic hepatitis B; endemic diseases; hepatitis C; pathogenicity; prognosis;     Show all 6 Subjects
Abstract:
... Screening and linkage to care are essential to achieve viral hepatitis elimination before 2030. The accurate identification of endemic areas is important for controlling diseases with geographic aggregation. Viral activity drives prognosis of chronic hepatitis B and hepatitis C virus infection. This screening was conducted in Chiayi County from 2018–2019. All residents aged 30 years or older were ...
DOI:
10.3390/v14020304

11. COVID-19 vaccine is correlated with favourable epidemiological indicators in the Auvergne-Rhône-Alpes region (France): An ecological study

Author:
Nicolas Romain-Scelle; Christelle Elias; Philippe Vanhems
Source:
Vaccine 2022 v.40 no.5 pp. 695-700
ISSN:
0264-410X
Subject:
viral load,   etc   ; COVID-19 infection; ecological studies; hospitals; reproduction; vaccination; vaccines; France;     Show all 8 Subjects
Abstract:
... COVID-19 vaccination has proven to be effective in preventing severe cases, reducing viral load, and transmissibility. The aim of this study was to evaluate the impact of vaccination 11 months after implementation on epidemiological indicators and the effective reproduction number in one French region. We plotted four indicators with vaccination coverage as the explaining variable and estimated th ...
DOI:
10.1016/j.vaccine.2021.12.036

12. DOLAVI Real-Life Study of Dolutegravir Plus Lamivudine in Naive HIV-1 Patients (48 Weeks)

Author:
Carmen Hidalgo-Tenorio; Juan Pasquau; David Vinuesa; Sergio Ferra; Alberto Terrón; Isabel SanJoaquín; Antoni Payeras; Onofre Juan Martínez; Miguel Ángel López-Ruz; Mohamed Omar; Javier de la Torre-Lima; Ana López-Lirola; Jesús Palomares; José Ramón Blanco; Marta Montero; Coral García-Vallecillos
Source:
Viruses 2022 v.14 no.3 pp. -
ISSN:
1999-4915
Subject:
viral load,   etc   ; anxiety; clinical trials; compliance; lamivudine; males; mutation; patients; sleep disorders;     Show all 9 Subjects
Abstract:
... Brief: Real-world data in naïve HIV-1 patients demonstrate that dolutegravir plus lamivudine in a multiple tablet regimen is effective, safe, and satisfactory; it causes moderately increasing weight and abdominal circumference and is administrable on a test-and-treat strategy. Background: Our objectives were to determine the real-life effectiveness and safety of DT with dolutegravir (50 mg/QD) plu ...
DOI:
10.3390/v14030524

13. Larger, more connected societies of ants have a higher prevalence of viruses

Author:
Anindita Brahma; Raphael Gray Leon; Gabriel Luis Hernandez; Yannick Wurm
Source:
Molecular ecology 2022 v.31 no.3 pp. 859-865
ISSN:
0962-1083
Subject:
viral load,   etc   ; Solenopsis invicta; group size; lead; metagenomics; pathogens; sequence analysis; society;     Show all 8 Subjects
Abstract:
... The benefits of cooperative living for foraging, nesting, defence and buffering environmental challenges lead animals with the most highly social lifestyles to dominate many ecosystems. However, living in larger, more highly connected groups should also increase the risks of pathogen exposure and transmission. While over long timescales selective responses could buffer the impacts of potential hig ...
DOI:
10.1111/mec.16284

14. Optimized formulation buffer preserves adeno-associated virus-9 infectivity after 4 °C storage and freeze/thawing cycling

Author:
Angela Chan; Carola J. Maturana; Esteban A. Engel
Source:
Journal of virological methods 2022 v.309 pp. 114598
ISSN:
0166-0934
Subject:
viral load,   etc   ; Dependoparvovirus; gene transfer; glycerol; nonionic surfactants; pathogenicity; sorbitol; viral genome;     Show all 8 Subjects
Abstract:
... Adeno-associated virus (AAV) have long been one of the most common and versatile vectors for in vitro and in vivo gene transfer. AAV production protocols are complex and time consuming, one key concern is the recovery and infectivity of viral vector after purification. The buffer used in the storage of AAV at 4 °C and − 80 °C is a crucial factor and methods to improve it have been thoroughly inves ...
DOI:
10.1016/j.jviromet.2022.114598

15. Boosting with variant-matched or historical mRNA vaccines protects against Omicron infection in mice

Author:
Baoling Ying; Suzanne M. Scheaffer; Bradley Whitener; Chieh-Yu Liang; Oleksandr Dmytrenko; Samantha Mackin; Kai Wu; Diana Lee; Laura E. Avena; Zhenlu Chong; James Brett Case; LingZhi Ma; Thu T.M. Kim; Caralyn E. Sein; Angela Woods; Daniela Montes Berrueta; Gwo-Yu Chang; Guillaume Stewart-Jones; Isabella Renzi; Yen-Ting Lai; Agata Malinowski; Andrea Carfi; Sayda M. Elbashir; Darin K. Edwards; Larissa B. Thackray; Michael S. Diamond
Source:
Cell 2022 v.185 no.9 pp. 1572-1587.e11
ISSN:
0092-8674
Subject:
viral load,   etc   ; Severe acute respiratory syndrome coronavirus 2; cytokines; inflammation; lungs; vaccination; vaccines;     Show all 7 Subjects
Abstract:
... The large number of spike substitutions in Omicron lineage variants (BA.1, BA.1.1., and BA.2) could jeopardize the efficacy of SARS-CoV-2 vaccines. We evaluated in mice the protective efficacy of the Moderna mRNA-1273 vaccine against BA.1 before or after boosting. Whereas two doses of mRNA-1273 vaccine induced high levels of neutralizing antibodies against historical WA1/2020 strains, lower levels ...
DOI:
10.1016/j.cell.2022.03.037

16. Evaluating the therapeutic efficacy of triptolide and (S)-10-hydroxycamptothecin on cutaneous and ocular Herpes Simplex Virus type-1 infections in mice

Author:
Nasrin Aliabadi; Marzieh Jamalidoust; Gholamreza Pouladfar; Negar Azarpira; Atoosa Ziyaeyan; Mazyar Ziyaeyan
Source:
Heliyon 2022 v.8 no.8 pp. e10348
ISSN:
2405-8440
Subject:
viral load,   etc   ; DNA; cornea; epithelium; herpes simplex; histopathology; immunohistochemistry; mice; viral genome; viruses;     Show all 10 Subjects
Abstract:
... The emergence of Acyclovir-Resistant Herpes Simplex Virus type-1, which is the result of clinical over usage calls for the urgent need of a novel anti-HSV agent. Hence, the activity of Triptolide (TP) and (S)-10-Hydroxycamptothecin (10-HCPT) were investigated as natural products in two infection models of HSV-1. The antiviral efficacy of TP and 10-HCPT was evaluated in mice ocular and cutaneous in ...
DOI:
10.1016/j.heliyon.2022.e10348

17. Ivermectin under scrutiny: a systematic review and meta-analysis of efficacy and possible sources of controversies in COVID-19 patients

Author:
Arman Shafiee; Mohammad Mobin Teymouri Athar; Omid Kohandel Gargari; Kyana Jafarabady; Sepehr Siahvoshi; Sayed-Hamidreza Mozhgani
Source:
Virology journal 2022 v.19 no.1 pp. 102
ISSN:
1743-422X
Subject:
viral load,   etc   ; COVID-19 infection; ivermectin; meta-analysis; mortality; risk; systematic review; virology;     Show all 8 Subjects
Abstract:
... BACKGROUND: We conducted a systematic review and meta-analysis to evaluate the efficacy of ivermectin for COVID-19 patients based on current peer-reviewed RCTs and to address disputes over the existing evidence. METHODS: MEDLINE (Pubmed), Scopus, Web of Science, Cochrane library, Google scholar and Clinicaltrials.gov were searched for RCTs assessing the efficacy of Ivermectin up to 20 February 202 ...
DOI:
10.1186/s12985-022-01829-8

18. Monitoring of porcine circovirus type 2 infection through air and surface samples in vaccinated and unvaccinated fattening farms

Author:
Gonzalo López‐Lorenzo; Cynthia López‐Novo; Alberto Prieto; Pablo Díaz; Rosario Panadero; Víctor Rodríguez‐Vega; Patrocinio Morrondo; Gonzalo Fernández; José Manuel Díaz‐Cao
Source:
Transboundary and emerging diseases 2022 v.69 no.3 pp. 1108-1117
ISSN:
1865-1674
Subject:
viral load,   etc   ; Porcine circovirus-2; air; blood; health status; herds; swine; viremia; viruses;     Show all 9 Subjects
Abstract:
... Air and surfaces of swine farms are the two alternative samples to obtain information about the health status of the herd. The aim of this study was to assess air and surface sampling for the detection of porcine circovirus type 2 (PCV2) in vaccinated and unvaccinated fattening farms, studying the relationship between the viral load in these samples with the viremia at herd level. Three swine fatt ...
DOI:
10.1111/tbed.14069

19. Impact of the potato inoculation date on potato virus Y load and viral distribution in daughter tubers at harvest

Author:
Frédéric Boulard; Gallien Rohaut; David Dupré; Florian Barbe Barrailh; Amélie Person; Melen Leclerc; Emmanuel Jacquot; Laurent Glais
Source:
Plant pathology 2022 v.71 no.6 pp. 1323-1334
ISSN:
0032-0862
Subject:
viral load,   etc   ; Potato virus Y; greenhouses; mature plants; plant pathology; plantlets; potatoes; viruses;     Show all 8 Subjects
Abstract:
... Aged plants are more difficult to infect than young plantlets. This modification of susceptibility is described as mature plant resistance (MPR). For potato virus Y (PVY), MPR is known to lead to low infection rates of plants inoculated at the postflowering stage and a decrease in the number of infected daughter tubers. However, the impact of inoculation date on the capacity of PVY to accumulate i ...
DOI:
10.1111/ppa.13573

20. Interferon Epsilon Signaling Confers Attenuated Zika Replication in Human Vaginal Epithelial Cells

Author:
James W. Mungin; Xin Chen; Bindong Liu
Source:
Pathogens 2022 v.11 no.8 pp. -
ISSN:
2076-0817
Subject:
viral load,   etc   ; RNA replication; Zika virus; epithelium; humans; interferons; protein synthesis; therapeutics; viruses;     Show all 9 Subjects
Abstract:
... Zika virus (ZIKV) is an emerging flavivirus that causes congenital birth defects and neurological compilations in the human host. Although ZIKV is primarily transmitted through infected mosquitos, recent studies reveal sexual contact as a potential transmission route. In vagina-bearing individuals, the vaginal epithelium constitutes the first line of defense against viruses. However, it is unclear ...
DOI:
10.3390/pathogens11080853