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1. High enrichment and near real-time quantification of airborne viruses using a wet-paper-based electrochemical immunosensor under an electrostatic field

Author:
Jyoti Bhardwaj; Nhan Dinh Ngo; Jaegil Lee; Jaesung Jang
Source:
Journal of hazardous materials 2023 v.442 pp. 130006
ISSN:
0304-3894
Subject:
viruses,   etc   ; antigen-antibody reactions; electrochemistry; electrodes; electrolytes; gels; immunosensors; liquids; virion;     Show all 9 Subjects
Abstract:
... Conventional airborne virus measurement usually requires appreciable sampling and detection times. Viral aerosols should also be collected or prepared in a liquid medium whose volume typically ranges from milliliters to tens of milliliters; hence, many sampling and detection steps need to be taken with the unit horizontal or immobile. Moreover, viral aerosols need to be sufficiently enriched, whic ...
DOI:
10.1016/j.jhazmat.2022.130006

2. Removal of virus aerosols by the combination of filtration and UV-C irradiation

Author:
Min Shang; Yadong Kong; Zhijuan Yang; Rong Cheng; Xiang Zheng; Yi Liu; Tongping Chen
Source:
Frontiers of environmental science & engineering 2023 v.17 no.3 pp. 27
ISSN:
2095-2201
Subject:
viruses,   etc   ; COVID-19 infection; aerosols; air; environmental science; filtration; irradiation; ultraviolet radiation;     Show all 8 Subjects
Abstract:
... The COVID-19 pandemic remains ever prevalent and afflicting—partially because one of its transmission pathways is aerosol. With the widely used central air conditioning systems worldwide, indoor virus aerosols can rapidly migrate, thus resulting in rapid infection transmission. It is therefore important to install microbial aerosol treatment units in the air conditioning systems, and we herein inv ...
DOI:
10.1007/s11783-023-1627-y

3. The community assembly for understanding the viral-assisted response of bacterial community to Cr-contamination in soils

Author:
Shuyue Liu; Yu Shi; Mingming Sun; Dan Huang; Wensheng Shu; Mao Ye
Source:
Journal of hazardous materials 2023 v.441 pp. 129975
ISSN:
0304-3894
Subject:
viruses,   etc   ; chromium; evolution; metagenomics; microbial communities; slags; soil; soil pollution; species diversity; variance; virus assembly; China;     Show all 12 Subjects
Abstract:
... Microbial community assembly contributes to the restoration and rehabilitation of the disturbed ecosystems. However, the viral assembly mechanism and its difference from bacterial communities under soil contamination have not been previously reported. Here, clean, light and heavy chromium (Cr)-contaminated soils were collected from two Cr slag sites and adjacent soils in northwestern and southwest ...
DOI:
10.1016/j.jhazmat.2022.129975

4. Smartphone recognition-based immune microparticles for rapid on-site visual data-sharing detection of Newcastle disease virus

Author:
Shenjunjie Lu; Binglin Ma; Haoran Zhou; Yayang Li; Zhihao Qiao; Tong Xiao; Husheng Li; Baijie Wang; Meihui Cui; Shufang Zhang; Jin Chang; Taofeng Du; Jing Liu; Hanjie Wang
Source:
Talanta 2023 v.252 pp. 123845
ISSN:
0039-9140
Subject:
viruses,   etc   ; Avian orthoavulavirus 1; antibodies; blood serum; color; human resources; humans; hydrogels; image analysis; industry; mobile telephones; nucleocapsid proteins;     Show all 12 Subjects
Abstract:
... Since the last century, animal viruses have posed great threats to the health of humans and the farming industry. Therefore, virus control is of great urgency, and regular, timely, and accurate detection is essential to it. Here, we designed a rapid on-site visual data-sharing detection method for the Newcastle disease virus with smartphone recognition-based immune microparticles. The detection me ...
DOI:
10.1016/j.talanta.2022.123845

5. Investigation of the latency of Cyprinid herpesvirus 2 in apparently healthy farmed gibel carp, Carassius auratus gibelio

Author:
Chang Wei; Chaonan Xu; Yuyu Sun; Jinzhou Li; Motohiko Sano; Qiang Li
Source:
Aquaculture 2023 v.562 pp. 738854
ISSN:
0044-8486
Subject:
viruses,   etc   ; Carassius auratus; Carassius gibelio; Cyprinid herpesvirus 2; DNA; aquaculture; carrier state; gene expression; genes; goldfish; kidneys; necrosis; pathogens; transcription (genetics);     Show all 14 Subjects
Abstract:
... Cyprinid herpesvirus 2 (CyHV-2) is a highly contagious pathogen that causes herpesviral haematopoietic necrosis (HVHN) disease to goldfish, Carassius auratus and gibel carp, C. auratus gibelio. In this study, the virus persistence or latency in apparently healthy farmed gibel carp and reactivation in vitro were investigated. All of the freshly dissected kidney tissues of 42 individuals of asymptom ...
DOI:
10.1016/j.aquaculture.2022.738854

6. Quantification of hepatitis E virus in raw pork livers using droplet digital RT-PCR

Author:
Kairui Wang; Libing Liu; Jinfeng Wang; Xiaoxia Sun; Qingan Han; Cang Zhou; Xiangdong Xu; Jianchang Wang
Source:
Food microbiology 2023 v.109 pp. 104114
ISSN:
0740-0020
Subject:
viruses,   etc   ; Orthohepevirus A; RNA; biosafety; detection limit; droplets; etiological agents; food microbiology; genes; hepatitis E; livestock; phylogeny; pork; reverse transcription; viral load; China;     Show all 16 Subjects
Abstract:
... Hepatitis E virus (HEV) is the causative agent of hepatitis E. Some of the rise in hepatitis E infection in China may be linked to undercooked pork. In this study, we established a reverse transcription droplet digital PCR (RT-ddPCR) method to detect HEV in raw pork livers. The detection limit of the assay for HEV RNA was as low as 1.81 copies/μL. The suggested approach was validated on 14 samples ...
DOI:
10.1016/j.fm.2022.104114

7. Research progress of biosensors for detection of SARS-CoV-2 variants based on ACE2

Author:
Hongshuai Wei; Congcong Zhang; Xin Du; Zhenguo Zhang
Source:
Talanta 2023 v.251 pp. 123813
ISSN:
0039-9140
Subject:
viruses,   etc   ; COVID-19 infection; Severe acute respiratory syndrome coronavirus 2; antibodies; biosensors; drug development; human health; mutation; pandemic; rapid methods; reverse transcriptase polymerase chain reaction;     Show all 11 Subjects
Abstract:
... Currently, the coronavirus disease 2019 (COVID-19) pandemic is ravaging the world, causing serious crisis in economy and human health. The top priority is the detection and drug development of the novel coronavirus. The gold standard for real-time diagnosis of coronavirus disease is the reverse transcription-polymerase chain reaction (RT-PCR), which is usually operatively complex and time-consumin ...
DOI:
10.1016/j.talanta.2022.123813

8. The crude extract from the flowers of Trollius chinensis Bunge exerts anti-influenza virus effects through modulation of the TLR3 signaling pathway

Author:
Yuxi Liang; Xiaoli Liu; Jingyan Hu; Songli Huang; Xin Ma; Xiaoyan Liu; Rufeng Wang; Xiuhua Hu
Source:
Journal of ethnopharmacology 2023 v.300 pp. 115743
ISSN:
0378-8741
Subject:
viruses,   etc   ; Influenza A virus; Oriental traditional medicine; Trollius chinensis; Western blotting; blood; cytoplasm; epithelium; fluorescent antibody technique; lungs; nasal cavity; pharmacology; pharyngitis; pneumonia; spleen; therapeutics;     Show all 16 Subjects
Abstract:
... The flowers of Trollius chinensis Bunge (Ranunculaceae) is a traditional Chinese medicine used to treat various inflammatory diseases, including upper respiratory infections, chronic tonsillitis, and pharyngitis. Recently, there has been growing research on the antiviral role of the flowers of T. chinensis Bunge. However, little is known about its anti-influenza virus effects and the underlying me ...
DOI:
10.1016/j.jep.2022.115743

9. COPMAN: A novel high-throughput and highly sensitive method to detect viral nucleic acids including SARS-CoV-2 RNA in wastewater

Author:
Yuka Adachi Katayama; Shin Hayase; Yoshinori Ando; Tomohiro Kuroita; Kazuya Okada; Ryo Iwamoto; Toru Yanagimoto; Masaaki Kitajima; Yusaku Masago
Source:
Science of the total environment 2023 v.856 pp. 158966
ISSN:
0048-9697
Subject:
viruses,   etc   ; COVID-19 infection; Japan; Pepper mild mottle virus; RNA; Severe acute respiratory syndrome coronavirus 2; automation; chlorides; coagulation; environment; feces; magnetism; pandemic; people; proteolysis; rivers; wastewater;     Show all 17 Subjects
Abstract:
... During the coronavirus disease 2019 (COVID-19) pandemic, wastewater-based epidemiology (WBE) attracted attention as an objective and comprehensive indicator of community infection that does not require individual inspection. Although several severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection methods from wastewater have been developed, there are obstacles to their social implem ...
DOI:
10.1016/j.scitotenv.2022.158966

10. Pepper autophagy related gene CaATG8e differentially regulates plant tolerance to heat and salt stress

Author:
Minmin Liang; Haiyan Li; Kang Yong; Guohong Huang; Yuling Guo; Minghui Lu
Source:
Scientia horticulturae 2023 v.308 pp. 111559
ISSN:
0304-4238
Subject:
viruses,   etc   ; Arabidopsis; Solanaceae; autophagosomes; autophagy; chlorophyll; computer software; electrolyte leakage; genes; heat; heat shock response; heat stress; heat tolerance; homeostasis; pepper; phylogeny; protein degradation; salt stress; salt tolerance;     Show all 19 Subjects
Abstract:
... As a conserved protein degradation system, autophagy plays important roles in removing or degrading misfolded and denatured proteins to maintain the homeostasis in cells. As a core member of autophagy, the involvement of ATG8 in plant tolerance to abiotic stress is paid more attention. In this study, by the SMART online tool, the Atg8 conserved domain was detected in CaATG8e, and by the MEGA11 sof ...
DOI:
10.1016/j.scienta.2022.111559

11. Ultrafast and absolute quantification of SARS-CoV-2 on food using hydrogel RT-LAMP without pre-lysis

Author:
Tao Yang; Dong Li; Yuhua Yan; Fatima-ezzahra Ettoumi; Ricardo A. Wu; Zisheng Luo; Hanry Yu; Xingyu Lin
Source:
Journal of hazardous materials 2023 v.442 pp. 130050
ISSN:
0304-3894
Subject:
viruses,   etc   ; RNA; Severe acute respiratory syndrome coronavirus 2; cold chain; crosslinking; food industry; hydrogels; monitoring; point-of-care systems; reverse transcription; reverse transcription loop-mediated isothermal amplification; storage quality;     Show all 12 Subjects
Abstract:
... With rapid growing of environmental contact infection, more and more attentions are focused on the precise and absolute quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus on cold chain foods via point-of-care test (POCT). In this work, we propose a hydrogel-mediated reverse transcription loop-mediated isothermal amplification (RT-LAMP) for ultrafast and absolute q ...
DOI:
10.1016/j.jhazmat.2022.130050

12. Establishment and characterization of a cell line from the brain of the Japanese flounder (Paralichthys olivaceus) and its application in the study of viral infection

Author:
Zhaodi Sun; Yuqin Ren; Yitong Zhang; Yucong Yang; Guixing Wang; Zhongwei He; Yufeng Liu; Wei Cao; Yufen Wang; Yuanshuai Fu; Jilun Hou
Source:
Aquaculture 2023 v.562 pp. 738825
ISSN:
0044-8486
Subject:
viruses,   etc   ; Andrias davidianus; Hirame rhabdovirus; Iridovirus; Paralichthys olivaceus; actin; aquaculture; brain; cell lines; chromosome number; chromosomes; diploidy; fish; gene expression; genes; host-pathogen relationships; interleukin-8; karyotyping; neural stem cells; temperature; transfection;     Show all 21 Subjects
Abstract:
... Fish cell lines are important tools in many biological studies. Here, we established a new Japanese flounder brain (JFB) cell line from the brain tissue of the Japanese flounder (Paralichthys olivaceus), which was successfully sub-cultured for over 120 passages, and consisted predominantly of fibroblast-like cells. Sequencing of the actin gene confirmed that JFB cells were derived from the Japanes ...
DOI:
10.1016/j.aquaculture.2022.738825

13. Genetics of novel leaf curl virus disease resistant pepper genotypes and antioxidative profile analysis of their progenies

Author:
Tejpal Singh Sran; Salesh Kumar Jindal; Abhishek Sharma; Neena Chawla
Source:
Scientia horticulturae 2023 v.308 pp. 111563
ISSN:
0304-4238
Subject:
viruses,   etc   ; Bemisia tabaci; Chilli leaf curl virus; ascorbate peroxidase; catechol oxidase; cultivars; disease resistance; genotype; leaf curling; pepper; peroxidase; phenylalanine ammonia-lyase; principal component analysis; recessive genes; superoxide dismutase; sweet peppers; tyrosine ammonia-lyase;     Show all 17 Subjects
Abstract:
... Pepper/Chilli leaf curl virus disease (PepLCVD/ChiLCVD) is the most destructive disease of pepper that seriously reduced pepper production. The LCVD is transmitted by vector whitefly (Bemisia tabaci) so efficiently that even single whitefly is able to transmit the virus. The effective way to manage the LCVD in pepper is to introduce LCVD resistance into agronomically desired cultivars, but the lac ...
DOI:
10.1016/j.scienta.2022.111563

14. Vectorial Release of Human RNA Viruses from Epithelial Cells

Author:
Sabine Chapuy-Regaud; Claire Allioux; Nicolas Capelli; Marion Migueres; Sébastien Lhomme; Jacques Izopet
Source:
Viruses 2022 v.14 no.2 pp. -
ISSN:
1999-4915
Subject:
RNA viruses; viruses,   etc   ; RNA; humans; mucosa; pathophysiology;     Show all 6 Subjects
Abstract:
... Epithelial cells are apico-basolateral polarized cells that line all tubular organs and are often targets for infectious agents. This review focuses on the release of human RNA virus particles from both sides of polarized human cells grown on transwells. Most viruses that infect the mucosa leave their host cells mainly via the apical side while basolateral release is linked to virus propagation wi ...
DOI:
10.3390/v14020231

15. Detection methods, epidemiological investigation, and host ranges of infectious precocity virus (IPV)

Author:
Caiyuan Zhao; Qijin Miu; Shanshan Liu; Dandan Zhou; Xinyi He; Jianhu Pang; Shaoping Weng; Jianguo He
Source:
Aquaculture 2023 v.562 pp. 738818
ISSN:
0044-8486
Subject:
viruses,   etc   ; Anisops; Carassius auratus; Charybdis feriatus; Eriocheir sinensis; Litopenaeus vannamei; Macrobrachium rosenbergii; Micropterus salmoides; Misgurnus anguillicaudatus; Oreochromis mossambicus; Pangasius bocourti; Penaeus monodon; Procambarus clarkii; Scylla paramamosain; aquaculture; brain; early development; epidemiological studies; insects; integument; nerve tissue; quantitative polymerase chain reaction; tissue tropism; China;     Show all 24 Subjects
Abstract:
... Giant freshwater prawn, Macrobrachium rosenbergii, is an important economically aquaculture species widely farmed in tropical and subtropical areas of the world. Infectious precocity virus (IPV) is a virus that results in slow growth and sexual precocity of farmed M. rosenbergii and causes significant economic losses. A TaqMan probe Quantitative Real-time PCR (qPCR) and a nested PCR assay for IPV ...
DOI:
10.1016/j.aquaculture.2022.738818

16. Inactivation of two SARS-CoV-2 virus surrogates by electron beam irradiation on large yellow croaker slices and their packaging surfaces

Author:
Zonghong Luo; Ke Ni; Yuancheng Zhou; Guanhong Chang; Jiangtao Yu; Chunling Zhang; Wenqi Yin; Dishi Chen; Shuwei Li; Shengyao Kuang; Peng Zhang; Kui Li; Junqing Bai; Xin Wang
Source:
Food control 2023 v.144 pp. 109340
ISSN:
0956-7135
Subject:
viruses,   etc   ; Larimichthys crocea; Porcine epidemic diarrhea virus; RNA; Severe acute respiratory syndrome coronavirus 2; Transmissible gastroenteritis virus; cold chain; color; culture media; decontamination; fish; food safety; genomics; irradiation; nonthermal processing; pH; quantitative polymerase chain reaction; risk reduction; total volatile basic nitrogen; transmission electron microscopy;     Show all 20 Subjects
Abstract:
... The detection of infectious SARS-CoV-2 in food and food packaging associated with the cold chain has raised concerns about the possible transmission pathway of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in foods transported through cold-chain logistics and the need for novel decontamination strategies. In this study, the effect of electron beam (E-beam) irradiation on the inactiv ...
DOI:
10.1016/j.foodcont.2022.109340

17. Investigating the Human Host—ssRNA Virus Interaction Landscape Using the SMEAGOL Toolbox

Author:
Avantika Lal; Mariana Galvao Ferrarini; Andreas J. Gruber
Source:
Viruses 2022 v.14 no.7 pp. -
ISSN:
1999-4915
Subject:
ssRNA viruses; viruses,   etc   ; RNA; antiviral agents; humans; selection pressure; viral genome;     Show all 7 Subjects
Abstract:
... Viruses have evolved numerous mechanisms to exploit the molecular machinery of their host cells, including the broad spectrum of host RNA-binding proteins (RBPs). However, the RBP interactomes of most viruses are largely unknown. To shed light on the interaction landscape of RNA viruses with human host cell RBPs, we have analysed 197 single-stranded RNA (ssRNA) viral genome sequences and found tha ...
DOI:
10.3390/v14071436

18. Preparation of substrates for microarray protein chips with different ending functional groups

Author:
Angus Shiue; Jenn-Han Chen; Cheng-Yen Hsiao; Shu-Mei Chang; Kuo-Yuan Hwa; Graham Leggett
Source:
Journal of immunological methods 2022 v.502 pp. 113218
ISSN:
0022-1759
Subject:
vertebrate viruses; viruses,   etc   ; antigens; fluorescence; fluorescent dyes; glass; protein microarrays; scanners; silicon;     Show all 9 Subjects
Abstract:
... Protein microarray chips are composed of three components, these are pre-treatment substrates, surface chemical modification, and immobilizing protein on substrate surfaces. In this study, self-assembly monolayers are used for surface chemical modification. Using this method, silanization on a glass and silicon chip is achieved, forming the terminal group substrates. Modification of the substrate ...
DOI:
10.1016/j.jim.2022.113218

19. Efficient measurement of airborne viable viruses using the growth-based virus aerosol concentrator with high flow velocities

Author:
Junbeom Jang; Jyoti Bhardwaj; Jaesung Jang
Source:
Journal of hazardous materials 2022 v.434 pp. 128873
ISSN:
0304-3894
Subject:
viruses,   etc   ; aerosols; air;     Show all 3 Subjects
Abstract:
... Growth tube collectors (GTCs) are used to sample virus aerosols because of their superior viable virus recovery among air samplers. However, a major limitation of such samplers is that they operate at low flow rates compared to many inertia-based air samplers. Herein, we demonstrated efficient measurements of airborne MS2 and T3 viruses using a GTC that can implement high flow velocities for highe ...
DOI:
10.1016/j.jhazmat.2022.128873

20. Synthesis of α-Ketoamides via Gold(I) Carbene Intermediates

Author:
Zuo Zuo Wu; Tapas Adak; Martin C. Dietl; Tao Wang; Chao Hu; Hongwei Shi; Petra Krämer; Matthias Rudolph; Frank Rominger; A. Stephen K. Hashmi
Source:
Organic letters 2022 v.24 no.24 pp. 4349-4353
ISSN:
1523-7052
Subject:
viruses,   etc   ; carbenes; hydrolysis;     Show all 3 Subjects
Abstract:
... α-Ketoamides have been found to be an important functional group in a broad spectrum of inhibitors such as the Corona virus and other viruses. Here we report an unprecedented gold-catalyzed 2-fold reaction of a bromoalkyne with anthranils. Hydrolysis of the initial product then directly leads to α-ketoamides. Water addition to the intermediate α-iminoimidoyl halides delivered α-ketoamides from a b ...
DOI:
10.1021/acs.orglett.2c01489